Carg11421 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11421
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	abscisic acid 8'-hydroxylase 1-like
Location	Carg_Chr16: 2884958 .. 2887377 (+)
RNA-Seq Expression	Carg11421
Synteny	Carg11421

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAACCAAACAACTCCGAAACATGTTCTGTTTCGCCGCCGTCATCGCCGCCTTCTTTCTTCTTCTTCTTCATTTTCTTTTCAAATTCTCCGCCCCTGCTCGCCGGAAACTTCCCCTTCCGCCGGGCTCAATGGGCTGGCCTTACATGGGTGAAACCCTCCAACTCTACTCGCAAGATCCCAATGTCTTCTTCGCCTCAAAGAAGAAGAGGTTCTTATTTGTTTCTCTGTTTGTTCATGTTTTTGGGTTTGTTTCTCAAATGGGTTTTGATTAATTTGATTAATTTGATTGATTTTGTTGTGTTTTTTTATTACAGATATGGTCCTATTTTCAAGTCCCACATATTGGGTTATCCTTGTGTGATGGTTTCTAGCCCTGAAGCTGCTAAGTTTGTGTTGGTCACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCCAGTAAAGAAAGGATGTTGGGCAAAAATGCCATCTTCTTTCACCAAGGTGACTACCATGCTAAATTACGACGCCTCGTTCTTCGAACATTCATGCCTGAAGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAGAACACTGTTCAATCATGGGATGGACAATTGATCAATACCTTCCAAGAAATGAAGATGGTATAATACAGTTCTTCCTCGATTCTTTACGTTAAAAACAGAGTAAAAACAGAGCAAAAACAGAGTAAATAACAGAGCATCTCTGTTTCTTATTCATATACTCTGTTTTTTTCGAATGGTTTGTATATTTGATTAATTATGTTCTTCTTGTTCTTCTTCAGTTTGCATTCGAGGTTTCTTTACTTTCGATATTTGGAAAAGAAGAAGCTCTTTACTTCGAAGACTTGAAGCGATGCTATTACACACTTGAAAAAGGGTACAATTCAATGCCGATAAACCTGCCAGGAACACTCTTCCACAAGGCCATGAAAGCAAGAAAAGAGCTAGCACATATCTTAACCAAGATCCTCTCAACCAGAAGGGAAATGAAGCGCGATCACAACGATCTCCTTGGATCTTTCATGGGTGAAAAGGAAGGCCTCACCGATGAACAGATCGCCGATAACGTCATCGGTCTAATCTTCGCTGCTCGGGATACGACCGCGAGTGTTCTAACATGGATCTTGAAGTATCTAGGAGAAAACCCAAGTGTTCTTCAAGCTGTCACTGTAAGAATCATCTCTAAACTTAAACCAAAACCCAAATCCCACTTTCTTTTCTTAGAGCTTAGAAATGAATTGAAATGAATTCTCCTCTGTTCTTGGGTTTATAGGATGAACAAGAAGCAATCATGAAGGAAAAACAGAGTGGAGATGATAATCTCACCTGGGCTGACACCAAAAACATGCCGATCACTTCAAGGGTGATTCAAGAAACTCTAAGAGTTGCTTCAGTTCTATCATTTACCTTCAGAGAAGCAGTGGAAGATGTAGAATTTGACGGTAAGAACACAAACCCAAGAAAAAAGAAAAAGTTAAATCCTGAAATCAAAACCCATTTTCATCTGTTTTCTGATTCAAAAACTCCTCATCTTCAGGCTATTTGATTCCAAAAGGATGGAAAGTTCTTCCTCTTTTCAGAAACATTCATCACAGCCCAGAAAATTTCCCTCAACCTGACAAGTTCGATCCTTCAAGATTCGAGGTAAAAACAGAGGAAACAAAGCACAGTGTATTTATTTATTAATTGCTTGCTTGCTTACTTCATTTAGAAAAACAAGAACATATTCTTCTTCTCTTTCCTTTTTCCCACACAAATTGGGTTAGCCCAAACATTCAATGATTGTGTTTATTATGATTGAAACAGGTCCCTCAAAAACCCAATACCTACATGCCATTTGGCAATGGAACCCACTCTTGTCCAGGAAATGAACTGGCCAAGCTCGAAATGCTCGTTCTTCTCCATCATTTAACCACCAAGTACAGGTCGGTAATTTCTCAATCTTTAACCTTCAAAATCATCTGATCCAAGAAGACCCATGTGATTGATTTGGTTGTTGGGTTGAAATTTATGCAGATGGAGTGTAGTGGGCGCTCAAGAAGGCATTCAATACGGTCCTTTTGCGCTTCCCCATAATGGGCTTCCCATTAAAATCTCTCTGAAGAAGTAAAAACAGAAGAACACAGAAACCCAGCAAAAAAAAAAAAGATCAAGAGAAGAGAATACGAAGATTGTTCCAAAACTTTGAGTCAAAAATTCAGAAAAAGTTTCTAAAAATAGAAATAGAGCAATGGAAAACTGTTGTCTAAGGTCAAATCAGAATGAGTGAGGTAAAGAAGAATCAGAAACAGAGGAAATGTGCTCTGTTATGGTTTTCTGGGCAAAAGGGGACAGTTTCTGACTCGCAAGAGAGTCATTATAGTTTGGATATACTTTAGAAAAACCCAACGGTCCTGATTTTTTTTT

mRNA sequence

ATAAACCAAACAACTCCGAAACATGTTCTGTTTCGCCGCCGTCATCGCCGCCTTCTTTCTTCTTCTTCTTCATTTTCTTTTCAAATTCTCCGCCCCTGCTCGCCGGAAACTTCCCCTTCCGCCGGGCTCAATGGGCTGGCCTTACATGGGTGAAACCCTCCAACTCTACTCGCAAGATCCCAATGTCTTCTTCGCCTCAAAGAAGAAGAGATATGGTCCTATTTTCAAGTCCCACATATTGGGTTATCCTTGTGTGATGGTTTCTAGCCCTGAAGCTGCTAAGTTTGTGTTGGTCACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCCAGTAAAGAAAGGATGTTGGGCAAAAATGCCATCTTCTTTCACCAAGGTGACTACCATGCTAAATTACGACGCCTCGTTCTTCGAACATTCATGCCTGAAGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAGAACACTGTTCAATCATGGGATGGACAATTGATCAATACCTTCCAAGAAATGAAGATGTTTGCATTCGAGGTTTCTTTACTTTCGATATTTGGAAAAGAAGAAGCTCTTTACTTCGAAGACTTGAAGCGATGCTATTACACACTTGAAAAAGGGTACAATTCAATGCCGATAAACCTGCCAGGAACACTCTTCCACAAGGCCATGAAAGCAAGAAAAGAGCTAGCACATATCTTAACCAAGATCCTCTCAACCAGAAGGGAAATGAAGCGCGATCACAACGATCTCCTTGGATCTTTCATGGGTGAAAAGGAAGGCCTCACCGATGAACAGATCGCCGATAACGTCATCGGTCTAATCTTCGCTGCTCGGGATACGACCGCGAGTGTTCTAACATGGATCTTGAAGTATCTAGGAGAAAACCCAAGTGTTCTTCAAGCTGTCACTGATGAACAAGAAGCAATCATGAAGGAAAAACAGAGTGGAGATGATAATCTCACCTGGGCTGACACCAAAAACATGCCGATCACTTCAAGGGTGATTCAAGAAACTCTAAGAGTTGCTTCAGTTCTATCATTTACCTTCAGAGAAGCAGTGGAAGATGTAGAATTTGACGGCTATTTGATTCCAAAAGGATGGAAAGTTCTTCCTCTTTTCAGAAACATTCATCACAGCCCAGAAAATTTCCCTCAACCTGACAAGTTCGATCCTTCAAGATTCGAGGTCCCTCAAAAACCCAATACCTACATGCCATTTGGCAATGGAACCCACTCTTGTCCAGGAAATGAACTGGCCAAGCTCGAAATGCTCGTTCTTCTCCATCATTTAACCACCAAGTACAGATGGAGTGTAGTGGGCGCTCAAGAAGGCATTCAATACGGTCCTTTTGCGCTTCCCCATAATGGGCTTCCCATTAAAATCTCTCTGAAGAAGTAAAAACAGAAGAACACAGAAACCCAGCAAAAAAAAAAAAGATCAAGAGAAGAGAATACGAAGATTGTTCCAAAACTTTGAGTCAAAAATTCAGAAAAAGTTTCTAAAAATAGAAATAGAGCAATGGAAAACTGTTGTCTAAGGTCAAATCAGAATGAGTGAGGTAAAGAAGAATCAGAAACAGAGGAAATGTGCTCTGTTATGGTTTTCTGGGCAAAAGGGGACAGTTTCTGACTCGCAAGAGAGTCATTATAGTTTGGATATACTTTAGAAAAACCCAACGGTCCTGATTTTTTTTT

Coding sequence (CDS)

ATGTTCTGTTTCGCCGCCGTCATCGCCGCCTTCTTTCTTCTTCTTCTTCATTTTCTTTTCAAATTCTCCGCCCCTGCTCGCCGGAAACTTCCCCTTCCGCCGGGCTCAATGGGCTGGCCTTACATGGGTGAAACCCTCCAACTCTACTCGCAAGATCCCAATGTCTTCTTCGCCTCAAAGAAGAAGAGATATGGTCCTATTTTCAAGTCCCACATATTGGGTTATCCTTGTGTGATGGTTTCTAGCCCTGAAGCTGCTAAGTTTGTGTTGGTCACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCCAGTAAAGAAAGGATGTTGGGCAAAAATGCCATCTTCTTTCACCAAGGTGACTACCATGCTAAATTACGACGCCTCGTTCTTCGAACATTCATGCCTGAAGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAGAACACTGTTCAATCATGGGATGGACAATTGATCAATACCTTCCAAGAAATGAAGATGTTTGCATTCGAGGTTTCTTTACTTTCGATATTTGGAAAAGAAGAAGCTCTTTACTTCGAAGACTTGAAGCGATGCTATTACACACTTGAAAAAGGGTACAATTCAATGCCGATAAACCTGCCAGGAACACTCTTCCACAAGGCCATGAAAGCAAGAAAAGAGCTAGCACATATCTTAACCAAGATCCTCTCAACCAGAAGGGAAATGAAGCGCGATCACAACGATCTCCTTGGATCTTTCATGGGTGAAAAGGAAGGCCTCACCGATGAACAGATCGCCGATAACGTCATCGGTCTAATCTTCGCTGCTCGGGATACGACCGCGAGTGTTCTAACATGGATCTTGAAGTATCTAGGAGAAAACCCAAGTGTTCTTCAAGCTGTCACTGATGAACAAGAAGCAATCATGAAGGAAAAACAGAGTGGAGATGATAATCTCACCTGGGCTGACACCAAAAACATGCCGATCACTTCAAGGGTGATTCAAGAAACTCTAAGAGTTGCTTCAGTTCTATCATTTACCTTCAGAGAAGCAGTGGAAGATGTAGAATTTGACGGCTATTTGATTCCAAAAGGATGGAAAGTTCTTCCTCTTTTCAGAAACATTCATCACAGCCCAGAAAATTTCCCTCAACCTGACAAGTTCGATCCTTCAAGATTCGAGGTCCCTCAAAAACCCAATACCTACATGCCATTTGGCAATGGAACCCACTCTTGTCCAGGAAATGAACTGGCCAAGCTCGAAATGCTCGTTCTTCTCCATCATTTAACCACCAAGTACAGATGGAGTGTAGTGGGCGCTCAAGAAGGCATTCAATACGGTCCTTTTGCGCTTCCCCATAATGGGCTTCCCATTAAAATCTCTCTGAAGAAGTAA

Protein sequence

MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK

Homology

[Close]

BLAST of Carg11421 vs. NCBI nr
Match: XP_022931288.1 (abscisic acid 8'-hydroxylase 1-like [Cucurbita moschata] >XP_023553532.1 abscisic acid 8'-hydroxylase 1-like [Cucurbita pepo subsp. pepo] >KAG7015108.1 Abscisic acid 8'-hydroxylase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 933.7 bits (2412), Expect = 6.0e-268
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

[Close]

BLAST of Carg11421 vs. NCBI nr
Match: KAG6577106.1 (Abscisic acid 8'-hydroxylase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 928.7 bits (2399), Expect = 1.9e-266
Identity = 458/460 (99.57%), Postives = 458/460 (99.57%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLF KAMKARKELAHILTKILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFRKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENP VLQAVTDE
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPCVLQAVTDE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

[Close]

BLAST of Carg11421 vs. NCBI nr
Match: XP_022985007.1 (abscisic acid 8'-hydroxylase 1-like [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 3.3e-266
Identity = 457/460 (99.35%), Postives = 459/460 (99.78%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFH+GD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHRGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMKEKQSGDDNLTW+DTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWSDTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

[Close]

BLAST of Carg11421 vs. NCBI nr
Match: XP_038877343.1 (abscisic acid 8'-hydroxylase CYP707A2-like [Benincasa hispida])

HSP 1 Score: 880.2 bits (2273), Expect = 7.9e-252
Identity = 431/454 (94.93%), Postives = 441/454 (97.14%), Query Frame = 0


Query: 7   VIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGP 66
           ++  F LL  H+LFKFSAPARRKLPLPPGSMGWPY+GETLQLYSQDPNVFFASKKKRYGP
Sbjct: 11  LLLLFVLLFFHYLFKFSAPARRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKKRYGP 70

Query: 67  IFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLR 126
           IFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFP SKERMLGKNAIFFHQGDYH+KLR
Sbjct: 71  IFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPVSKERMLGKNAIFFHQGDYHSKLR 130

Query: 127 RLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEAL 186
           RLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQ INTFQEMKMFAFEVSLLSIFGK+EAL
Sbjct: 131 RLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQFINTFQEMKMFAFEVSLLSIFGKDEAL 190

Query: 187 YFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLL 246
           YFEDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELAHIL KILSTRREMKRDHNDLL
Sbjct: 191 YFEDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAHILNKILSTRREMKRDHNDLL 250

Query: 247 GSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMK 306
           GSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT EQEAIMK
Sbjct: 251 GSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAEQEAIMK 310

Query: 307 EKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLP 366
           EKQSGDDNLTWADTKNMPITSRVIQETLR+ASVLSFTFREAVEDVEFDGYLIPKGWKVLP
Sbjct: 311 EKQSGDDNLTWADTKNMPITSRVIQETLRMASVLSFTFREAVEDVEFDGYLIPKGWKVLP 370

Query: 367 LFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLT 426
           LFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLT
Sbjct: 371 LFRNIHHSPEIFPQPNKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLT 430

Query: 427 TKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           TKYRW+VVGAQEGIQYGPFALP NGLPI+ISLKK
Sbjct: 431 TKYRWNVVGAQEGIQYGPFALPLNGLPIRISLKK 464

[Close]

BLAST of Carg11421 vs. NCBI nr
Match: XP_022136913.1 (abscisic acid 8'-hydroxylase 1-like [Momordica charantia])

HSP 1 Score: 867.1 bits (2239), Expect = 6.9e-248
Identity = 429/460 (93.26%), Postives = 435/460 (94.57%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           M CF      FF  LL  L K SA  RRKLPLPPGSMGWPYMGET QLYSQDPNVFFASK
Sbjct: 1   MLCFLCFSLFFFFFLLRSLSKSSAAPRRKLPLPPGSMGWPYMGETFQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPE IRNIVPSIESIAKNTVQSW+GQ INTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEKIRNIVPSIESIAKNTVQSWEGQSINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GK+EALYFEDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELA+ILTKILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELANILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMK KQSGDDNLTW DTKNMP+TSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKAKQSGDDNLTWGDTKNMPVTSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FP P+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPHPEKFDPSRFEVAQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIRISLKK 460

[Close]

BLAST of Carg11421 vs. ExPASy Swiss-Prot
Match: K4CI52 (Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.3e-209
Identity = 356/461 (77.22%), Postives = 413/461 (89.59%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           M C    I +  LLL+H +FKF A A +KLPLPPG++G PY+GET QLYSQ+PNVFFASK
Sbjct: 6   MLCLFTFI-SLTLLLIHSIFKFLAFASKKLPLPPGTLGLPYIGETFQLYSQNPNVFFASK 65

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
            K+YG IFK++ILG PCVM+SSPEAAK VLVTKA+LFKPTFPASKERMLGK AIFFHQGD
Sbjct: 66  VKKYGSIFKTYILGCPCVMISSPEAAKQVLVTKANLFKPTFPASKERMLGKQAIFFHQGD 125

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLR+LVL+ F P++IRNI+P IESIA  +++S+ G+LINT+QEMK + F V+L+SIF
Sbjct: 126 YHAKLRKLVLQAFKPDSIRNIIPDIESIAITSLESFQGRLINTYQEMKTYTFNVALISIF 185

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GK+E LY E+LK+CYY LEKGYNSMPINLPGTLF+KAMKARKELA I+ KI+STRREMK 
Sbjct: 186 GKDEFLYREELKKCYYILEKGYNSMPINLPGTLFNKAMKARKELAKIVAKIISTRREMKI 245

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DH DLLGSFMG+KEGLTDEQIADNVIG+IFAARDTTASVLTWILKYLGENPSVLQAVT+E
Sbjct: 246 DHGDLLGSFMGDKEGLTDEQIADNVIGVIFAARDTTASVLTWILKYLGENPSVLQAVTEE 305

Query: 301 QEAIMKEKQ-SGDDN-LTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLI 360
           QE IM++K+ +G++  L W DT+ MP+T+RVIQETLRVAS+LSFTFREAVEDVEF+GYLI
Sbjct: 306 QENIMRKKEVNGEEKVLNWQDTRQMPMTTRVIQETLRVASILSFTFREAVEDVEFEGYLI 365

Query: 361 PKGWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEM 420
           PKGWKVLPLFRNIHHSP+NFP+P+KFDPSRFEV  KPNT+MPFGNG HSCPGN+LAKLE+
Sbjct: 366 PKGWKVLPLFRNIHHSPDNFPEPEKFDPSRFEVSPKPNTFMPFGNGVHSCPGNDLAKLEI 425

Query: 421 LVLLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
           L+L+HHLTTKYRWS+VG Q GIQYGPFALP NGLPIK+SLK
Sbjct: 426 LILVHHLTTKYRWSMVGPQNGIQYGPFALPQNGLPIKLSLK 465

[Close]

BLAST of Carg11421 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 3.1e-206
Identity = 341/450 (75.78%), Postives = 394/450 (87.56%), Query Frame = 0


Query: 10  AFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIFK 69
           + FL  L  L         KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+KRYG +FK
Sbjct: 14  SLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFK 73

Query: 70  SHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLV 129
           +H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYHAKLR+LV
Sbjct: 74  THVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV 133

Query: 130 LRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYFE 189
           LR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFGK+E LY E
Sbjct: 134 LRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRE 193

Query: 190 DLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGSF 249
           DLKRCYY LEKGYNSMP+NLPGTLFHK+MKARKEL+ IL +ILS RR+    HNDLLGSF
Sbjct: 194 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSF 253

Query: 250 MGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEKQ 309
           MG+KE LTDEQIADN+IG+IFAARDTTASV++WILKYL ENP+VL+AVT+EQ AI K+K+
Sbjct: 254 MGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKE 313

Query: 310 SGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFR 369
            G ++LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKGWKVLPLFR
Sbjct: 314 EG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 373

Query: 370 NIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKY 429
           NIHHS + F  P KFDPSRFEV  KPNT+MPFGNGTHSCPGNELAKLEM +++HHLTTKY
Sbjct: 374 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 433

Query: 430 RWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
            WS+VGA +GIQYGPFALP NGLPI ++ K
Sbjct: 434 SWSIVGASDGIQYGPFALPQNGLPIVLARK 462

[Close]

BLAST of Carg11421 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 5.1e-201
Identity = 335/451 (74.28%), Postives = 387/451 (85.81%), Query Frame = 0


Query: 9   AAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIF 68
           AA FL LL F+      +  KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K++RYG +F
Sbjct: 13  AALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVF 72

Query: 69  KSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRL 128
           K+H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYH+KLR+L
Sbjct: 73  KTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKL 132

Query: 129 VLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYF 188
           VLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI GK+E  Y 
Sbjct: 133 VLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYR 192

Query: 189 EDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGS 248
           EDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELA IL  ILS RR+    H DLLGS
Sbjct: 193 EDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGS 252

Query: 249 FMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEK 308
           FM +K GLTDEQIADN+IG+IFAARDTTASVLTWILKYL +NP+VL+AVT+EQ AI K+K
Sbjct: 253 FMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDK 312

Query: 309 QSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLF 368
           + G ++LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKGWKVLPLF
Sbjct: 313 KEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLF 372

Query: 369 RNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTK 428
           RNIHH+ + F  P KFDPSRFEV  KPNT+MPFG+G HSCPGNELAKLE+ VL+HHLTTK
Sbjct: 373 RNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTK 432

Query: 429 YRWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
           YRWS+VG  +GIQYGPFALP NGLPI +  K
Sbjct: 433 YRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

[Close]

BLAST of Carg11421 vs. ExPASy Swiss-Prot
Match: Q09J79 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 8.4e-188
Identity = 321/461 (69.63%), Postives = 379/461 (82.21%), Query Frame = 0


Query: 11  FFLLLLHFLFKFSAPARRK-----------LPLPPGSMGWPYMGETLQLY-SQDPNVFFA 70
           F  +L  FL   +   RR+           LPLPPGSMGWPY+GET QLY S++PNVFF 
Sbjct: 7   FVCVLAPFLLVCAVRGRRRQAGSSEAAACGLPLPPGSMGWPYVGETFQLYSSKNPNVFFN 66

Query: 71  SKKKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQ 130
            K+ +YGPIFK+HILG PCVMVSSPEAA+FVLVT+AHLFKPTFPASKERMLG  AIFF Q
Sbjct: 67  KKRNKYGPIFKTHILGCPCVMVSSPEAARFVLVTQAHLFKPTFPASKERMLGPQAIFFQQ 126

Query: 131 GDYHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLS 190
           GDYHA LRR+V R F PE+IR  VP+IE+IA  ++ SWDGQ +NTFQEMK +A  V+LLS
Sbjct: 127 GDYHAHLRRIVSRAFSPESIRASVPAIEAIALRSLHSWDGQFVNTFQEMKTYALNVALLS 186

Query: 191 IFGKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREM 250
           IFG+EE  Y E+LK+CY TLEKGYNSMP+NLPGTLFHKAMKARK L  I+  I+S RRE 
Sbjct: 187 IFGEEEMRYIEELKQCYLTLEKGYNSMPVNLPGTLFHKAMKARKRLGAIVAHIISARRER 246

Query: 251 KRDHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT 310
           +R  NDLLGSF+  +E LTD QIADNVIG+IFAARDTTASVLTW++K+LG++P+VL+AVT
Sbjct: 247 QRG-NDLLGSFVDGREALTDAQIADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVT 306

Query: 311 DEQEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLI 370
           +EQ  I KEK++  + L+WADT+ M +TSRVIQET+RVAS+LSFTFREAVEDVE+ GYLI
Sbjct: 307 EEQLQIAKEKEASGEPLSWADTRRMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLI 366

Query: 371 PKGWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEM 430
           PKGWKVLPLFRNIHH+P++FP P+KFDPSRFEV  KPNT+MPFGNGTHSCPGNELAKLEM
Sbjct: 367 PKGWKVLPLFRNIHHNPDHFPCPEKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEM 426

Query: 431 LVLLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
           LVL HHL TKYRWS   ++ G+Q+GPFALP NGLP+  + K
Sbjct: 427 LVLFHHLATKYRWSTSKSESGVQFGPFALPLNGLPMSFTRK 466

[Close]

BLAST of Carg11421 vs. ExPASy Swiss-Prot
Match: Q05JG2 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 8.4e-188
Identity = 321/461 (69.63%), Postives = 379/461 (82.21%), Query Frame = 0


Query: 11  FFLLLLHFLFKFSAPARRK-----------LPLPPGSMGWPYMGETLQLY-SQDPNVFFA 70
           F  +L  FL   +   RR+           LPLPPGSMGWPY+GET QLY S++PNVFF 
Sbjct: 7   FVCVLAPFLLVCAVRGRRRQAGSSEAAACGLPLPPGSMGWPYVGETFQLYSSKNPNVFFN 66

Query: 71  SKKKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQ 130
            K+ +YGPIFK+HILG PCVMVSSPEAA+FVLVT+AHLFKPTFPASKERMLG  AIFF Q
Sbjct: 67  KKRNKYGPIFKTHILGCPCVMVSSPEAARFVLVTQAHLFKPTFPASKERMLGPQAIFFQQ 126

Query: 131 GDYHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLS 190
           GDYHA LRR+V R F PE+IR  VP+IE+IA  ++ SWDGQ +NTFQEMK +A  V+LLS
Sbjct: 127 GDYHAHLRRIVSRAFSPESIRASVPAIEAIALRSLHSWDGQFVNTFQEMKTYALNVALLS 186

Query: 191 IFGKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREM 250
           IFG+EE  Y E+LK+CY TLEKGYNSMP+NLPGTLFHKAMKARK L  I+  I+S RRE 
Sbjct: 187 IFGEEEMRYIEELKQCYLTLEKGYNSMPVNLPGTLFHKAMKARKRLGAIVAHIISARRER 246

Query: 251 KRDHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT 310
           +R  NDLLGSF+  +E LTD QIADNVIG+IFAARDTTASVLTW++K+LG++P+VL+AVT
Sbjct: 247 QRG-NDLLGSFVDGREALTDAQIADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVT 306

Query: 311 DEQEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLI 370
           +EQ  I KEK++  + L+WADT+ M +TSRVIQET+RVAS+LSFTFREAVEDVE+ GYLI
Sbjct: 307 EEQLQIAKEKEASGEPLSWADTRRMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLI 366

Query: 371 PKGWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEM 430
           PKGWKVLPLFRNIHH+P++FP P+KFDPSRFEV  KPNT+MPFGNGTHSCPGNELAKLEM
Sbjct: 367 PKGWKVLPLFRNIHHNPDHFPCPEKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEM 426

Query: 431 LVLLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
           LVL HHL TKYRWS   ++ G+Q+GPFALP NGLP+  + K
Sbjct: 427 LVLFHHLATKYRWSTSKSESGVQFGPFALPLNGLPMSFTRK 466

[Close]

BLAST of Carg11421 vs. ExPASy TrEMBL
Match: A0A6J1EY25 (abscisic acid 8'-hydroxylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437516 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 2.9e-268
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

[Close]

BLAST of Carg11421 vs. ExPASy TrEMBL
Match: A0A6J1J3P6 (abscisic acid 8'-hydroxylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483101 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.6e-266
Identity = 457/460 (99.35%), Postives = 459/460 (99.78%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFH+GD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHRGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMKEKQSGDDNLTW+DTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWSDTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

[Close]

BLAST of Carg11421 vs. ExPASy TrEMBL
Match: A0A6J1C8U1 (abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC111008490 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.4e-248
Identity = 429/460 (93.26%), Postives = 435/460 (94.57%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           M CF      FF  LL  L K SA  RRKLPLPPGSMGWPYMGET QLYSQDPNVFFASK
Sbjct: 1   MLCFLCFSLFFFFFLLRSLSKSSAAPRRKLPLPPGSMGWPYMGETFQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPE IRNIVPSIESIAKNTVQSW+GQ INTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEKIRNIVPSIESIAKNTVQSWEGQSINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GK+EALYFEDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELA+ILTKILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELANILTKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QEAIMK KQSGDDNLTW DTKNMP+TSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKAKQSGDDNLTWGDTKNMPVTSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FP P+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPHPEKFDPSRFEVAQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIRISLKK 460

[Close]

BLAST of Carg11421 vs. ExPASy TrEMBL
Match: A0A1S3BTM7 (abscisic acid 8'-hydroxylase 1 OS=Cucumis melo OX=3656 GN=CYP707A1 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.2e-247
Identity = 423/460 (91.96%), Postives = 441/460 (95.87%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL   +LFKFSAPA RKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           D++DLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVT E
Sbjct: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+IMK K++G+DNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTKYRW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

[Close]

BLAST of Carg11421 vs. ExPASy TrEMBL
Match: A0A0A0KX97 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056600 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.3e-247
Identity = 420/460 (91.30%), Postives = 443/460 (96.30%), Query Frame = 0


Query: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL  H+LFKFSAP+RRKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MYVFVSLLAFFFLLFFHYLFKFSAPSRRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRRE KR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAEILNKILSTRRETKR 240

Query: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300
           +++DLLGSFMGEKEGLTD+QIADNVIGLIFAARDTTASVLTWI+KYLGENPS+LQAVT E
Sbjct: 241 EYDDLLGSFMGEKEGLTDKQIADNVIGLIFAARDTTASVLTWIVKYLGENPSILQAVTAE 300

Query: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+I+K K++GDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIVKSKENGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 461
           LLHHLTTK+RW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKFRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

[Close]

BLAST of Carg11421 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 719.2 bits (1855), Expect = 2.2e-207
Identity = 341/450 (75.78%), Postives = 394/450 (87.56%), Query Frame = 0


Query: 10  AFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIFK 69
           + FL  L  L         KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+KRYG +FK
Sbjct: 14  SLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFK 73

Query: 70  SHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLV 129
           +H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYHAKLR+LV
Sbjct: 74  THVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV 133

Query: 130 LRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYFE 189
           LR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFGK+E LY E
Sbjct: 134 LRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRE 193

Query: 190 DLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGSF 249
           DLKRCYY LEKGYNSMP+NLPGTLFHK+MKARKEL+ IL +ILS RR+    HNDLLGSF
Sbjct: 194 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSF 253

Query: 250 MGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEKQ 309
           MG+KE LTDEQIADN+IG+IFAARDTTASV++WILKYL ENP+VL+AVT+EQ AI K+K+
Sbjct: 254 MGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKE 313

Query: 310 SGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFR 369
            G ++LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKGWKVLPLFR
Sbjct: 314 EG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 373

Query: 370 NIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKY 429
           NIHHS + F  P KFDPSRFEV  KPNT+MPFGNGTHSCPGNELAKLEM +++HHLTTKY
Sbjct: 374 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 433

Query: 430 RWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
            WS+VGA +GIQYGPFALP NGLPI ++ K
Sbjct: 434 SWSIVGASDGIQYGPFALPQNGLPIVLARK 462

[Close]

BLAST of Carg11421 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 701.8 bits (1810), Expect = 3.6e-202
Identity = 335/451 (74.28%), Postives = 387/451 (85.81%), Query Frame = 0


Query: 9   AAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIF 68
           AA FL LL F+      +  KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K++RYG +F
Sbjct: 13  AALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVF 72

Query: 69  KSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRL 128
           K+H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYH+KLR+L
Sbjct: 73  KTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKL 132

Query: 129 VLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYF 188
           VLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI GK+E  Y 
Sbjct: 133 VLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYR 192

Query: 189 EDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGS 248
           EDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELA IL  ILS RR+    H DLLGS
Sbjct: 193 EDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGS 252

Query: 249 FMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEK 308
           FM +K GLTDEQIADN+IG+IFAARDTTASVLTWILKYL +NP+VL+AVT+EQ AI K+K
Sbjct: 253 FMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDK 312

Query: 309 QSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLF 368
           + G ++LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKGWKVLPLF
Sbjct: 313 KEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLF 372

Query: 369 RNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTK 428
           RNIHH+ + F  P KFDPSRFEV  KPNT+MPFG+G HSCPGNELAKLE+ VL+HHLTTK
Sbjct: 373 RNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTK 432

Query: 429 YRWSVVGAQEGIQYGPFALPHNGLPIKISLK 460
           YRWS+VG  +GIQYGPFALP NGLPI +  K
Sbjct: 433 YRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

[Close]

BLAST of Carg11421 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-194
Identity = 325/446 (72.87%), Postives = 378/446 (84.75%), Query Frame = 0


Query: 10  AFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIFK 69
           + FL  L  L         KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+KRYG +FK
Sbjct: 14  SLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFK 73

Query: 70  SHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLV 129
           +H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYHAKLR+LV
Sbjct: 74  THVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLV 133

Query: 130 LRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYFE 189
           LR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFGK+E LY E
Sbjct: 134 LRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRE 193

Query: 190 DLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGSF 249
           DLKRCYY LEKGYNSMP+NLPGTLFHK+MKARKEL+ IL +ILS RR+    HNDLLGSF
Sbjct: 194 DLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSF 253

Query: 250 MGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEKQ 309
           MG+KE LTDEQIADN+IG+IFAARDTTASV++WILKYL ENP+VL+AVT+EQ AI K+K+
Sbjct: 254 MGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKE 313

Query: 310 SGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFR 369
            G ++LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKGWKVLPLFR
Sbjct: 314 EG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 373

Query: 370 NIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKY 429
           NIHHS + F  P KFDPSRFEV  KPNT+MPFGNGTHSCPGNELAKLEM +++HHLTTKY
Sbjct: 374 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 433

Query: 430 RW-SVVGAQEGIQYGPFALPHNGLPI 455
           R+  ++    G   G    P    P+
Sbjct: 434 RFGQLLERATGFSMGHSRFPKTDCPL 458

[Close]

BLAST of Carg11421 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 655.2 bits (1689), Expect = 3.9e-188
Identity = 315/422 (74.64%), Postives = 364/422 (86.26%), Query Frame = 0


Query: 9   AAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKRYGPIF 68
           AA FL LL F+      +  KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K++RYG +F
Sbjct: 13  AALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVF 72

Query: 69  KSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRL 128
           K+H+LG PCVM+SSPEAAKFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDYH+KLR+L
Sbjct: 73  KTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKL 132

Query: 129 VLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKEEALYF 188
           VLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI GK+E  Y 
Sbjct: 133 VLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYR 192

Query: 189 EDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRDHNDLLGS 248
           EDLKRCYY LEKGYNSMPINLPGTLFHKAMKARKELA IL  ILS RR+    H DLLGS
Sbjct: 193 EDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGS 252

Query: 249 FMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDEQEAIMKEK 308
           FM +K GLTDEQIADN+IG+IFAARDTTASVLTWILKYL +NP+VL+AVT+EQ AI K+K
Sbjct: 253 FMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDK 312

Query: 309 QSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLF 368
           + G ++LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKGWKVLPLF
Sbjct: 313 KEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLF 372

Query: 369 RNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTK 428
           RNIHH+ + F  P KFDPSRFEV  KPNT+MPFG+G HSCPGNELAKLE+ VL+HHLTTK
Sbjct: 373 RNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTK 432

Query: 429 YR 431
           YR
Sbjct: 433 YR 433

[Close]

BLAST of Carg11421 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 569.7 bits (1467), Expect = 2.1e-162
Identity = 278/460 (60.43%), Postives = 357/460 (77.61%), Query Frame = 0


Query: 5   AAVIAAFFLLLLHFLFKFSAP-ARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASKKKR 64
           A +     ++++  LFK+      ++L LPPGSMG PY+GETL+LY+++PN FFA+++ +
Sbjct: 21  ALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNK 80

Query: 65  YGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHA 124
           YG IFK+HILG PCVM+SSPEAA+ VLV+KAHLFKPT+P SKERM+G  A+FFHQG YH+
Sbjct: 81  YGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHS 140

Query: 125 KLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQ-LINTFQEMKMFAFEVSLLSIFG- 184
            L+RLV  +FMP A+R  V  IE +   T+ SW  Q  INT + MK +AF+V+++S FG 
Sbjct: 141 TLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGD 200

Query: 185 KEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKRD 244
           KEE    + +K  Y  LE+GYNSMP++LPGTLFHK+MKAR EL+  L K++  RRE  R+
Sbjct: 201 KEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGRE 260

Query: 245 HNDLLGSFMGEKE----GLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAV 304
              LLG  +G K+    GL+D QIADN+IG+IFAA DTTASVLTW+LKYL ++P++LQ V
Sbjct: 261 EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEV 320

Query: 305 TDEQEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYL 364
           + EQ +I ++ +  +  ++W DT+ MP+T+RVIQETLR ASVLSFTFREAV+DVE+DGYL
Sbjct: 321 SREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYL 380

Query: 365 IPKGWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLE 424
           IPKGWKVLPLFR IHHS E FP P+KFDPSRFEV  KP TYMPFGNG HSCPG+ELAKLE
Sbjct: 381 IPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLE 440

Query: 425 MLVLLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKIS 458
           ML+LLHHLTT +RW V+G +EGIQYGPF +P  GLPI+++
Sbjct: 441 MLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVT 480

The following BLAST results are available for this feature:

BLAST of Carg11421 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022931288.1	6.0e-268	100.00	abscisic acid 8'-hydroxylase 1-like [Cucurbita moschata] >XP_023553532.1 abscisi...	[more]
KAG6577106.1	1.9e-266	99.57	Abscisic acid 8'-hydroxylase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022985007.1	3.3e-266	99.35	abscisic acid 8'-hydroxylase 1-like [Cucurbita maxima]	[more]
XP_038877343.1	7.9e-252	94.93	abscisic acid 8'-hydroxylase CYP707A2-like [Benincasa hispida]	[more]
XP_022136913.1	6.9e-248	93.26	abscisic acid 8'-hydroxylase 1-like [Momordica charantia]	[more]

BLAST of Carg11421 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K4CI52	1.3e-209	77.22	Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
Q949P1	3.1e-206	75.78	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ...	[more]
Q9FH76	5.1e-201	74.28	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ...	[more]
Q09J79	8.4e-188	69.63	Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q05JG2	8.4e-188	69.63	Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]

BLAST of Carg11421 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EY25	2.9e-268	100.00	abscisic acid 8'-hydroxylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11143751...	[more]
A0A6J1J3P6	1.6e-266	99.35	abscisic acid 8'-hydroxylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483101 ...	[more]
A0A6J1C8U1	3.4e-248	93.26	abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC1110084...	[more]
A0A1S3BTM7	2.2e-247	91.96	abscisic acid 8'-hydroxylase 1 OS=Cucumis melo OX=3656 GN=CYP707A1 PE=3 SV=1	[more]
A0A0A0KX97	6.3e-247	91.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056600 PE=3 SV=1	[more]

BLAST of Carg11421 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19230.1	2.2e-207	75.78	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.1	3.6e-202	74.28	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.2	2.1e-194	72.87	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.2	3.9e-188	74.64	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT2G29090.1	2.1e-162	60.43	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 328..339 score: 37.55 coord: 399..408 score: 47.36 coord: 271..288 score: 35.59 coord: 408..419 score: 40.5
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..435 e-value: 2.4E-81 score: 273.8
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 398..408 score: 44.06 coord: 61..80 score: 29.79 coord: 280..306 score: 23.54 coord: 367..391 score: 28.63 coord: 260..277 score: 26.45 coord: 408..431 score: 26.83 coord: 327..345 score: 26.01
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..458 e-value: 1.5E-124 score: 418.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..456
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..457
None	No IPR available	PANTHER	PTHR24286:SF249	ABSCISIC ACID 8'-HYDROXYLASE CYP707A2	coord: 8..457
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 401..410

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11421-RA	Carg11421-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009687	abscisic acid metabolic process
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0010295	(+)-abscisic acid 8'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen