Carg11313 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11313
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSerine hydroxymethyltransferase
LocationCarg_Chr16: 2138059 .. 2142598 (-)
RNA-Seq ExpressionCarg11313
SyntenyCarg11313
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCTTCTTCCTCCTCCTGTCTCTCTCTCTCTCAGGGCCTCCATTCTGTAGGGAAATCTCACAGTCCTAGGGTTGTCGCTGTTTCTGGCTTTTCCATGGAAGTTTCGTTGAGTTCAATGCCACAATCTTTCAAGCTTGGCCGTTCTACCGACCTCAATCGCCTCTGCCCCTGCCGCTGTTCTTGTTGTTCTTGATATTGTTCCTGATTCGGAGCTTCTGTTGGGGTTTGGCTTTGCCAAATTTTTCTATCTTTGATTGTTTTTGTTTACAGGAGGTGTAATCTTTTGGGGACTGGAATTCGTTTGATAGGCTTAAGACTTTGATATTTGTGGCTTTGTTCGTAGTTAATGGATTTGTCTAATTCCCATTCCAATCTTTCTCTTGGAATCTCATCGCATGCTTCGCCACCGCACCGCGTTCAGATTGCTGATGATTCCATTCTGCTTCATCTCGAATCTTCTCGACAGGAACCCACGTTTTCGTCCCCCCCAATCCCTCTTCAGTTGATGGAACCGCTGACGGAGAGTCACAGAGACGAGGTTGGAGATGTGGAGAGAGAAGACGATGTCTACGACGACAGGGATGTAGAAGAGTTTCGTATTCTGGGCCATTCTATGTGCTTGAAGCGCCGAAGAGATAGTGAATCTTCGTTCTCGTCGAGTTCTGCAAAGAGAAATTCGTGTGAACGTGATTTAGAGACACGAAGAAGTGCTGTACGTTCGTGGGGTAACCAGACCCTTCGAGCCGCGGACCCTAACCTATTTGGAATTATGGAGAAGGAGAAACAGAGGCAAATCAAGGGCATTGAATTGATTGCTTCTGAGAATTATGTGTGTCGTGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCCGAAGGTATGCCCGGTGCGAGGTACTACTGTGGAAATCAATATATAGATGAGATTGAAACGCTATGTCGTGAGCGCGCATTGGCAGCGTTTGGTCTTGATTCTGATAGTTGGGGGGTTAACGTGCAACCTTATTCATGCACATCCGCGAACTTTGCTGTCTACACTGGTCTGCTACTCCCGGGTGATCGAATCATGGGGTTGGATACTCCTTCGGGTGGTAACACTAGTCATGGCTACTGCACCCCGAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTCGAGAGTTTTCCATATAAGGTTAATCCGCAAACTGGGTATATCGATTATGATAAGCTTGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGGGGGAGCTCGTATCCTCGTGAATTGGATTATGGAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATCAGTGGCCTTGTTGCTGCTAAGGTTTGAATTTTTCTTCACCATTAGCGATGCTACGGCCCTTGCTCGAATGACTGTTTATTAATCTTTTGGTTTCTCGGCTCACCAAAGTTTTTGGTTTTCTTTTTATATGTTCTAATTGCTATCATGTCCTCATTCTCTTGGGTTGCAATTTAGTTGGTCGTTAACATAATTATGATTTTTGAATTGTTTTACCTGACACTCGGAAACGGCGTCAAAGGGTGGTTGTATGGAATTTCTTTGTGATAGTAAAATGTAACTGACCAAGGGATCTCGGTAGACACTGATTTTAGGTCTTTACCTTAGTTTAGCAGTGTGTTGGGACAAGATGTCTTGACCCCTTTGATTTTATGAGAATTGAAACCTTTGAGAAACACTCTGGACTGCTTTTGAATCTTTAAGCTTATGAATTATGGTATTGTTCTTATTGAAAAATAATTTTTCTTTCTACTGGATTGTATTATACAGTTCGTTCAACATCGTGTTTATGCAATTTTCAGTGGGTTTCTTTTACATCTGTAATTATTTTTTCACTTGCATTGTTTGGGCCAGTCCATCACTACTACTTCATGTGTAGTCCTGTAATTTTAGACAGAATTATCCCTACTCTGTTTATGTATTGATTTATTTGGATTTGAATTTTATTACAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCCACTACTCATAAGAGCCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGCAGTAAGCCGAGGAGGAGAGCAATGCTTCTGTATCAGGGAAATGAAGCTAACCAATATGACTTTGAGGAGAAAATAAACTTCGCTGTTTTTCCATCCTTACAAGGTGGACCACACAATAACCATATTGCTGCGCTTGCTGTGGCTCTAAAACAAGTGGCTACTCTGGAGTACAAAGCATACATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTAGCTTCTGCTCTACTCCGAAAGAACTACAAGCTTGTAACTGGTGGCACAGATAATCACATGGTACTCTGGGACCTAAGGTCCTTGGGATTGACAGGTAATAATTTTTATCCAAAGTTTGCAGATTGATGTGAACATGATACATTCTAGGTGCACAATTAGTTTTTTGAAAGAGGCGCACTAAAACATTGCTCATGCACAGTGAGGGTAGATTATGTTATGCACTGTGGGTTGTGATTAGAGGAAATGGTATATTATTATATTTCATGAGTTACACAATTTGGAATGAATAGATTTGTTTTGTGTCAACTGTATAACCATTCAAAACAGGATACTGTAGTTGAGTACGCTTAAAAGGTATCTTAATTATATTAGTAAGGTGTTAGAAATTAAAGAATTTGATTGGCAAGAAATGAGAGAATTTTTCAAGATAATTCTTAATCAGTCGGTCATAAAAAAAGGACGTGTCAGAAGTGATGAAAAGATGAATAAGGCATGCTATTTTAAGGCAAGGCAAGTCTTAGCATTAGGGTGCAAGTAAAATCAAGCTTAATCAGTCAGCAGTGGTTTGGGATGTTGTGTGCCGACCATCAACTGAGTCGACTGATTAAGCGTTTCCTATTTCCTTCATTTTATAACTTCTTTCCCTTGAAAGAAAATTAAGATAAGCGAAAAATTGCCTAATTGAACCGACCTAACTGCAAATCAAGTTAATTTAGTTGGCATGGTTACTACATAATGTGTGGTCGGATGCAGTTTTTCATTTTTTCATAGATTATATTAGTGGTTCAGTGTCGATTTTGCCCATAAATTGAGGCATTGCTCCCCTACTTAGAAGACAAGTCTTGTCTTCTTAGGACACATCCTCCTGTATTGAGCCTAATACTGAGATCATAAGTATAATTTTGACGTCATGAAATAGCTTTTCTGACTTTTCAACTGGATACAGGACATTGTTTCATTGATAAAAGAATTTTAATATCATAGTGCGCTATTTAGTATCCTGCAGGAAGATCCATTTCTCTGGCTTCTTTATTTGTATTATACGATGTAAAACAAATGAAGATAGATTCTGAACGATTTTAATCTATTTCAACTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTTCCATATTCTTATACGTCTGTTTCTTCTAAGTTCTAGCTTTGATGGTATATGTGGCTGCTTACTCTAATTCTCCATTCATTATACGTTGTGTAATCTTTTTGATGCCATGCTTATTGTAGGTAAAAACTTTGAGCAAGTTTGTGAGATGTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTACTCCTGGAGGAGTAAGGATCGGTGAGTTTCCCTCTTATATTTCATCTTTTGGGGATATCCTAAGATTGAAAAGCTCAGTATGCATTGATTATTCTAATAAATTAGCTGTCTGTCTGTTATCCCTGCAGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAAGTAATTGTAGAATTTCTTCATATAGCCGCTCAAATTGCAATTGTTGTTCAGCGAGAGCATGGGAAGTTGCCGAAAGCTTTTCTTAGGGGCCTTCAAAGTAATAATGAAGTTGTTGAGCTTGGATACCGTGTTGCAAGTTTTTCAGCTAAATTTGCAATGCCTGGCCTTGAAACTTGAATGGAGGGTGACCATTTGTTGTCCCTAAAAAAAGAAAAATAATATCTGCTGCTCATCATGCCTTCCATATGAAATCTTGAAGCTTCTCCCTCTCTTGGGGTTGCTGTTTATTCAGATGCCTGCATTACTTGTCATCTTATTGTTTTGAGAAGGGCCTTTGCAATCATCAGAAGAGAATTTAGTAATGCCGATCTTTTCTTCTACAAGGCCGAATGTGTACGATGGATAGAGGCGTGATGAGCAGTTTTTTGGTGGTGTCATTTTTGGGATCCAATCAACTGAAGAAATTGGTGGCTACGAGGGAATATAATTTAAAAAAATATAGTACTCTATCAGAGACCGGGAAGAATGCTTTGGACCTGATTGTTTATGGCCACGGCTGAGGAATCATTTTGCCAGCTTGATGTGTGATGATGTGGTAGGAAGTACGCACAATGCTGGTCAATTTCCTTTGATCAGAGTTCCATTGTGTAAGTTCTTGCTACATTTCCTCCCTCATACAAATTAT

mRNA sequence

TCTTCTTCTTCCTCCTCCTGTCTCTCTCTCTCTCAGGGCCTCCATTCTGTAGGGAAATCTCACAGTCCTAGGGTTGTCGCTGTTTCTGGCTTTTCCATGGAAGTTTCGTTGAGTTCAATGCCACAATCTTTCAAGCTTGGCCGTTCTACCGACCTCAATCGCCTCTGCCCCTGCCGCTGTTCTTGTTGTTCTTGATATTGTTCCTGATTCGGAGCTTCTGTTGGGGTTTGGCTTTGCCAAATTTTTCTATCTTTGATTGTTTTTGTTTACAGGAGGTGTAATCTTTTGGGGACTGGAATTCGTTTGATAGGCTTAAGACTTTGATATTTGTGGCTTTGTTCGTAGTTAATGGATTTGTCTAATTCCCATTCCAATCTTTCTCTTGGAATCTCATCGCATGCTTCGCCACCGCACCGCGTTCAGATTGCTGATGATTCCATTCTGCTTCATCTCGAATCTTCTCGACAGGAACCCACGTTTTCGTCCCCCCCAATCCCTCTTCAGTTGATGGAACCGCTGACGGAGAGTCACAGAGACGAGGTTGGAGATGTGGAGAGAGAAGACGATGTCTACGACGACAGGGATGTAGAAGAGTTTCGTATTCTGGGCCATTCTATGTGCTTGAAGCGCCGAAGAGATAGTGAATCTTCGTTCTCGTCGAGTTCTGCAAAGAGAAATTCGTGTGAACGTGATTTAGAGACACGAAGAAGTGCTGTACGTTCGTGGGGTAACCAGACCCTTCGAGCCGCGGACCCTAACCTATTTGGAATTATGGAGAAGGAGAAACAGAGGCAAATCAAGGGCATTGAATTGATTGCTTCTGAGAATTATGTGTGTCGTGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCCGAAGGTATGCCCGGTGCGAGGTACTACTGTGGAAATCAATATATAGATGAGATTGAAACGCTATGTCGTGAGCGCGCATTGGCAGCGTTTGGTCTTGATTCTGATAGTTGGGGGGTTAACGTGCAACCTTATTCATGCACATCCGCGAACTTTGCTGTCTACACTGGTCTGCTACTCCCGGGTGATCGAATCATGGGGTTGGATACTCCTTCGGGTGGTAACACTAGTCATGGCTACTGCACCCCGAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTCGAGAGTTTTCCATATAAGGTTAATCCGCAAACTGGGTATATCGATTATGATAAGCTTGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGGGGGAGCTCGTATCCTCGTGAATTGGATTATGGAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATCAGTGGCCTTGTTGCTGCTAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCCACTACTCATAAGAGCCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGCAGTAAGCCGAGGAGGAGAGCAATGCTTCTGTATCAGGGAAATGAAGCTAACCAATATGACTTTGAGGAGAAAATAAACTTCGCTGTTTTTCCATCCTTACAAGGTGGACCACACAATAACCATATTGCTGCGCTTGCTGTGGCTCTAAAACAAGTGGCTACTCTGGAGTACAAAGCATACATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTAGCTTCTGCTCTACTCCGAAAGAACTACAAGCTTGTAACTGGTGGCACAGATAATCACATGGTACTCTGGGACCTAAGGTCCTTGGGATTGACAGGTAAAAACTTTGAGCAAGTTTGTGAGATGTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTACTCCTGGAGGAGTAAGGATCGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAAGTAATTGTAGAATTTCTTCATATAGCCGCTCAAATTGCAATTGTTGTTCAGCGAGAGCATGGGAAGTTGCCGAAAGCTTTTCTTAGGGGCCTTCAAAGTAATAATGAAGTTGTTGAGCTTGGATACCGTGTTGCAAGTTTTTCAGCTAAATTTGCAATGCCTGGCCTTGAAACTTGAATGGAGGGTGACCATTTGTTGTCCCTAAAAAAAGAAAAATAATATCTGCTGCTCATCATGCCTTCCATATGAAATCTTGAAGCTTCTCCCTCTCTTGGGGTTGCTGTTTATTCAGATGCCTGCATTACTTGTCATCTTATTGTTTTGAGAAGGGCCTTTGCAATCATCAGAAGAGAATTTAGTAATGCCGATCTTTTCTTCTACAAGGCCGAATGTGTACGATGGATAGAGGCGTGATGAGCAGTTTTTTGGTGGTGTCATTTTTGGGATCCAATCAACTGAAGAAATTGGTGGCTACGAGGGAATATAATTTAAAAAAATATAGTACTCTATCAGAGACCGGGAAGAATGCTTTGGACCTGATTGTTTATGGCCACGGCTGAGGAATCATTTTGCCAGCTTGATGTGTGATGATGTGGTAGGAAGTACGCACAATGCTGGTCAATTTCCTTTGATCAGAGTTCCATTGTGTAAGTTCTTGCTACATTTCCTCCCTCATACAAATTAT

Coding sequence (CDS)

ATGGATTTGTCTAATTCCCATTCCAATCTTTCTCTTGGAATCTCATCGCATGCTTCGCCACCGCACCGCGTTCAGATTGCTGATGATTCCATTCTGCTTCATCTCGAATCTTCTCGACAGGAACCCACGTTTTCGTCCCCCCCAATCCCTCTTCAGTTGATGGAACCGCTGACGGAGAGTCACAGAGACGAGGTTGGAGATGTGGAGAGAGAAGACGATGTCTACGACGACAGGGATGTAGAAGAGTTTCGTATTCTGGGCCATTCTATGTGCTTGAAGCGCCGAAGAGATAGTGAATCTTCGTTCTCGTCGAGTTCTGCAAAGAGAAATTCGTGTGAACGTGATTTAGAGACACGAAGAAGTGCTGTACGTTCGTGGGGTAACCAGACCCTTCGAGCCGCGGACCCTAACCTATTTGGAATTATGGAGAAGGAGAAACAGAGGCAAATCAAGGGCATTGAATTGATTGCTTCTGAGAATTATGTGTGTCGTGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCCGAAGGTATGCCCGGTGCGAGGTACTACTGTGGAAATCAATATATAGATGAGATTGAAACGCTATGTCGTGAGCGCGCATTGGCAGCGTTTGGTCTTGATTCTGATAGTTGGGGGGTTAACGTGCAACCTTATTCATGCACATCCGCGAACTTTGCTGTCTACACTGGTCTGCTACTCCCGGGTGATCGAATCATGGGGTTGGATACTCCTTCGGGTGGTAACACTAGTCATGGCTACTGCACCCCGAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTCGAGAGTTTTCCATATAAGGTTAATCCGCAAACTGGGTATATCGATTATGATAAGCTTGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGGGGGAGCTCGTATCCTCGTGAATTGGATTATGGAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTGATGTGTGATATGGCTCAAATCAGTGGCCTTGTTGCTGCTAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCCACTACTCATAAGAGCCTTCGAGGTCCAAGGGGAGGTATAATTTTTTATAGGAAGGGCAGTAAGCCGAGGAGGAGAGCAATGCTTCTGTATCAGGGAAATGAAGCTAACCAATATGACTTTGAGGAGAAAATAAACTTCGCTGTTTTTCCATCCTTACAAGGTGGACCACACAATAACCATATTGCTGCGCTTGCTGTGGCTCTAAAACAAGTGGCTACTCTGGAGTACAAAGCATACATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTAGCTTCTGCTCTACTCCGAAAGAACTACAAGCTTGTAACTGGTGGCACAGATAATCACATGGTACTCTGGGACCTAAGGTCCTTGGGATTGACAGGTAAAAACTTTGAGCAAGTTTGTGAGATGTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAATGGAATTATTACTCCTGGAGGAGTAAGGATCGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAAGTAATTGTAGAATTTCTTCATATAGCCGCTCAAATTGCAATTGTTGTTCAGCGAGAGCATGGGAAGTTGCCGAAAGCTTTTCTTAGGGGCCTTCAAAGTAATAATGAAGTTGTTGAGCTTGGATACCGTGTTGCAAGTTTTTCAGCTAAATTTGCAATGCCTGGCCTTGAAACTTGA

Protein sequence

MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTESHRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRRSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET
Homology
BLAST of Carg11313 vs. NCBI nr
Match: KAG6576983.1 (Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015003.1 Serine hydroxymethyltransferase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR
Sbjct: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Carg11313 vs. NCBI nr
Match: XP_023552257.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 586/588 (99.66%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLGIS HASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSHSNLSLGISLHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVGD EREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Carg11313 vs. NCBI nr
Match: XP_022922715.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVGD EREDDVYDDRDVEEFRILGHSMCLKRRRDSESS SSSS+KRNSCERDLETRR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRA DPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIAIVVQRE+GKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Carg11313 vs. NCBI nr
Match: XP_022984574.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 572/588 (97.28%), Postives = 577/588 (98.13%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNS+SNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVG  ERE    DDRDVEEF ILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR
Sbjct: 61  HRDEVGGAERE----DDRDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGS+PR  AMLLYQGNE+NQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIA+VVQREHGKLPK FLRGLQS+NEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLET 584

BLAST of Carg11313 vs. NCBI nr
Match: XP_022149204.1 (serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149207.1 serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 529/587 (90.12%), Postives = 554/587 (94.38%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLG SSHASPPHR QIADDS+LLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDE G+ EREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S KR SCE D++ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLR ADPNLFGIMEKEK RQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQ+AT EYKAYMQQVKKNAQALASALLRK Y+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKN+E+VCE+C ITLNKITI+GDNGII PGGVRIGTPAMTSRGCLESDFE IV+FLH AA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           QIAI VQREHGKLPKA+L+GLQ+NNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Carg11313 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 1.7e-230
Identity = 402/593 (67.79%), Postives = 474/593 (79.93%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGIS-SHAS----PPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLME 60
           MDLS S +N  LG   SHAS    P  R  IADDSI L ++ S +    +  PIPLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PLTESHRDEVGDVERED--DVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSS-AKRNSC 120
              E  +    D  ++D     D ++ E FRILGH MCLKR+RD     + S   KR+S 
Sbjct: 61  QKAE--KTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSI 120

Query: 121 -ERDLETRRSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALG 180
            + DLE+RR+AVR+WG+Q +  ADP++  +MEKEKQRQ++GIELIASEN+VCRAVMEALG
Sbjct: 121 GDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFESFPYKVNPQTGYIDYDK
Sbjct: 241 VYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDK 300

Query: 301 LEERALDFRPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LE++ALD+RPKILICGGSSYPR+ D+ R RQIADKCGAVLMCDMA ISGLVA KEC +PF
Sbjct: 301 LEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPF 360

Query: 361 GYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSL 420
            +CD+VTSTTHK LRGPRGGIIFYR+G K R++       + +  YD EEKINFAVFPSL
Sbjct: 361 DHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYKAY+QQ+KKNAQALA+ALLR+  +LVTGGTDNH++L
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLL 480

Query: 481 WDLRSLGLTGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEV 540
           WDL  +GLTGK +E+VCEMCHITLNK  IFGDNG I+PGGVRIGTPAMT+RGC+ESDFE 
Sbjct: 481 WDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFET 540

Query: 541 IVEFLHIAAQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMP 585
           + +FL  AAQI   +QREHGK  K F++ L +N ++ EL  RV +F+ ++ MP
Sbjct: 541 MADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Carg11313 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.4e-226
Identity = 395/598 (66.05%), Postives = 478/598 (79.93%), Query Frame = 0

Query: 6   SHSNLSLGI-SSHASP---PHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEP----- 65
           + S+LSLG  SSHA P   P R+ IADDSI L ++SS +  +   PP+PLQL+E      
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  -----LTESHRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRR--DSESSFSSSSAKR 125
                + E   + VGD   +DD  D R+ E+F +LGH M LKR R  +S S  SSS  KR
Sbjct: 65  GSCSRVVEEDDEVVGD--NDDD--DQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKR 124

Query: 126 NSCERDLETRRSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEA 185
              +  +E+RR+ VR+WGNQ++  ADP +   MEKEKQRQ +GIELIASEN+VCRAVMEA
Sbjct: 125 FVVDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEA 184

Query: 186 LGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSAN 245
           LGSHLTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSAN
Sbjct: 185 LGSHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSAN 244

Query: 246 FAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDY 305
           FAV+TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFESFPYKV+P+TGYIDY
Sbjct: 245 FAVFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDY 304

Query: 306 DKLEERALDFRPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVS 365
           DKLEE+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +
Sbjct: 305 DKLEEKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPN 364

Query: 366 PFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFP 425
           PF YCD+VTSTTHKSLRGPRGGIIFY++G KP+++++ L       QYDFEEKINF+VFP
Sbjct: 365 PFDYCDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFP 424

Query: 426 SLQGGPHNNHIAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHM 485
           SLQGGPHNNHIAALA+ALKQ A+ EYK YM+QVKKNA+ALASAL+ +  KL+TGGTDNH+
Sbjct: 425 SLQGGPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHL 484

Query: 486 VLWDLRSLGLTGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDF 545
           +LWDL  LGLTGK +E+VCEMCHIT+NK+ IF +NG+I+PGGVRIG+PAMTSRGCLE +F
Sbjct: 485 LLWDLTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEF 544

Query: 546 EVIVEFLHIAAQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           E + +FL+ AAQIA   QREHGKL K  L+ +    E+ +L  +V +F+ +FAMP  +
Sbjct: 545 ETMADFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Carg11313 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.8e-172
Identity = 293/463 (63.28%), Postives = 354/463 (76.46%), Query Frame = 0

Query: 123 VRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L + DP +  ++EKEK+RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF  D  +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FES PYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DY RFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP ++        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGK 482
           LAVALKQ  T  +K Y +QVK NA AL + L+ K Y++VT GT+NH+VLWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQI 542
             E++C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I EFL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPG 586
            + +Q+ +GKL K F +GL +N ++ +L   V  FSA + MPG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Carg11313 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.5e-169
Identity = 285/463 (61.56%), Postives = 345/463 (74.51%), Query Frame = 0

Query: 123 VRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+ PYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DY RFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP ++        E   YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGK 482
           LAVALKQV    +K Y +QVK NA  LAS L+ K Y LVT GTDNH++LWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQI 542
             E+VCE+C+ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I EFLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPG 586
            + +Q ++GK+ K F +GL +N E+ E+   V  F+  F MPG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Carg11313 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 8.0e-151
Identity = 264/460 (57.39%), Postives = 331/460 (71.96%), Query Frame = 0

Query: 127 GNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 186
           GN  L+  D  +F +M +EK RQ KG+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 187 YYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGL 246
           YY G + +DE+ETLC++RAL AF LD   WGVNVQPYS + ANFAVYT LL P DRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 247 DTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKILIC 306
           D PSGG+ +HGY T + +K+S +SIFFES PY++    G IDY +LEE AL F+PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 307 GGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLR 366
           G S+YPRE DY R R IADK GA LMCDMA  SGLVAA+   SPF YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 367 GPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAALAVA 426
           GPR GIIF+R+G +      +   G E  +YD E KINFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKR------VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 427 LKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGKNFEQ 486
           LK+  + E+K Y  QVKKNA A+ +AL+ K YKLVT GTDNH++LWDLR   LTG  FE+
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 487 VCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQIAIVV 546
             ++ +IT+NK  + GD   I+PGG+RIG+ A+TSRG  E+DFE I +FL     I++ +
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 547 QREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGL 587
           Q   GK    F+  +  + E+++L   V  FS+KF +PG+
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPGI 457

BLAST of Carg11313 vs. ExPASy TrEMBL
Match: A0A6J1E9K3 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE=3 SV=1)

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVGD EREDDVYDDRDVEEFRILGHSMCLKRRRDSESS SSSS+KRNSCERDLETRR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRA DPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIAIVVQRE+GKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Carg11313 vs. ExPASy TrEMBL
Match: A0A6J1JAX6 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 572/588 (97.28%), Postives = 577/588 (98.13%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNS+SNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDEVG  ERE    DDRDVEEF ILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR
Sbjct: 61  HRDEVGGAERE----DDRDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGS+PR  AMLLYQGNE+NQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 589
           QIA+VVQREHGKLPK FLRGLQS+NEVVELGYRVASFSAKFAMPGLET
Sbjct: 541 QIAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLET 584

BLAST of Carg11313 vs. ExPASy TrEMBL
Match: A0A6J1D7A8 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 PE=3 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 529/587 (90.12%), Postives = 554/587 (94.38%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLSNSHSNLSLG SSHASPPHR QIADDS+LLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HRDE G+ EREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S KR SCE D++ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLR ADPNLFGIMEKEK RQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQ+AT EYKAYMQQVKKNAQALASALLRK Y+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKN+E+VCE+C ITLNKITI+GDNGII PGGVRIGTPAMTSRGCLESDFE IV+FLH AA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           QIAI VQREHGKLPKA+L+GLQ+NNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Carg11313 vs. ExPASy TrEMBL
Match: A0A6J1FV71 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 527/587 (89.78%), Postives = 552/587 (94.04%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLS SHSNLSLG SSHASPPHR QIADDS+LLHL+SSR+E ++SS P+PLQLME  TES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HR++ GD ER+DD YDDR+VEEFRILGHSMCLKRRRD ESS SS SAKR SCE D+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLR ADPNLFGIMEKEKQRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVAT EYKAYMQQVKKNAQALA+ALLRK YKLVTGGTDNHM+LWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKN+E VCE+CHITLNKITIFGDNGII PGGVR+GTPAMTSRGCLESDFE I +FLH AA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           QIAI VQRE+GKLPK +L+GL SNNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Carg11313 vs. ExPASy TrEMBL
Match: A0A6J1IXT5 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 526/587 (89.61%), Postives = 551/587 (93.87%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60
           MDLS SHSNLSLG SSHASPPHR QIADDS+LLHL+SSR+E ++SS P+PLQLME  TES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120
           HR++ GD ER+DD YDDR+VEEFRILGH MCLKRRRD ESS SSSSAKR SCE D+ETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLR ADPNLFGIMEKEKQRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480
           AALAVALKQVAT EYKAYMQQVKKNAQALA+ALLRK YKLVTGGTDNHM+LWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540
           GKN+E VCE+CHITLNKITIFGDNGII PGGVR+GTPAMTSRGCLESDFE I +FLH AA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           QIAI VQRE+GKLPK +L+GL SNNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Carg11313 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 800.0 bits (2065), Expect = 1.2e-231
Identity = 402/593 (67.79%), Postives = 474/593 (79.93%), Query Frame = 0

Query: 1   MDLSNSHSNLSLGIS-SHAS----PPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLME 60
           MDLS S +N  LG   SHAS    P  R  IADDSI L ++ S +    +  PIPLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PLTESHRDEVGDVERED--DVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSS-AKRNSC 120
              E  +    D  ++D     D ++ E FRILGH MCLKR+RD     + S   KR+S 
Sbjct: 61  QKAE--KTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSI 120

Query: 121 -ERDLETRRSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALG 180
            + DLE+RR+AVR+WG+Q +  ADP++  +MEKEKQRQ++GIELIASEN+VCRAVMEALG
Sbjct: 121 GDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALG 180

Query: 181 SHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFA 240
           SHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFA
Sbjct: 181 SHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFA 240

Query: 241 VYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDK 300
           VYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFESFPYKVNPQTGYIDYDK
Sbjct: 241 VYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDK 300

Query: 301 LEERALDFRPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPF 360
           LE++ALD+RPKILICGGSSYPR+ D+ R RQIADKCGAVLMCDMA ISGLVA KEC +PF
Sbjct: 301 LEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPF 360

Query: 361 GYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSL 420
            +CD+VTSTTHK LRGPRGGIIFYR+G K R++       + +  YD EEKINFAVFPSL
Sbjct: 361 DHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSL 420

Query: 421 QGGPHNNHIAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVL 480
           QGGPHNNHIAALA+ALKQVAT EYKAY+QQ+KKNAQALA+ALLR+  +LVTGGTDNH++L
Sbjct: 421 QGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLL 480

Query: 481 WDLRSLGLTGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEV 540
           WDL  +GLTGK +E+VCEMCHITLNK  IFGDNG I+PGGVRIGTPAMT+RGC+ESDFE 
Sbjct: 481 WDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFET 540

Query: 541 IVEFLHIAAQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMP 585
           + +FL  AAQI   +QREHGK  K F++ L +N ++ EL  RV +F+ ++ MP
Sbjct: 541 MADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Carg11313 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 785.4 bits (2027), Expect = 3.2e-227
Identity = 395/598 (66.05%), Postives = 478/598 (79.93%), Query Frame = 0

Query: 6   SHSNLSLGI-SSHASP---PHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEP----- 65
           + S+LSLG  SSHA P   P R+ IADDSI L ++SS +  +   PP+PLQL+E      
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  -----LTESHRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRR--DSESSFSSSSAKR 125
                + E   + VGD   +DD  D R+ E+F +LGH M LKR R  +S S  SSS  KR
Sbjct: 65  GSCSRVVEEDDEVVGD--NDDD--DQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKR 124

Query: 126 NSCERDLETRRSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEA 185
              +  +E+RR+ VR+WGNQ++  ADP +   MEKEKQRQ +GIELIASEN+VCRAVMEA
Sbjct: 125 FVVDSGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEA 184

Query: 186 LGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSAN 245
           LGSHLTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSAN
Sbjct: 185 LGSHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSAN 244

Query: 246 FAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDY 305
           FAV+TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFESFPYKV+P+TGYIDY
Sbjct: 245 FAVFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDY 304

Query: 306 DKLEERALDFRPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVS 365
           DKLEE+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +
Sbjct: 305 DKLEEKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPN 364

Query: 366 PFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFP 425
           PF YCD+VTSTTHKSLRGPRGGIIFY++G KP+++++ L       QYDFEEKINF+VFP
Sbjct: 365 PFDYCDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFP 424

Query: 426 SLQGGPHNNHIAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHM 485
           SLQGGPHNNHIAALA+ALKQ A+ EYK YM+QVKKNA+ALASAL+ +  KL+TGGTDNH+
Sbjct: 425 SLQGGPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHL 484

Query: 486 VLWDLRSLGLTGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDF 545
           +LWDL  LGLTGK +E+VCEMCHIT+NK+ IF +NG+I+PGGVRIG+PAMTSRGCLE +F
Sbjct: 485 LLWDLTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEF 544

Query: 546 EVIVEFLHIAAQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 588
           E + +FL+ AAQIA   QREHGKL K  L+ +    E+ +L  +V +F+ +FAMP  +
Sbjct: 545 ETMADFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Carg11313 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 605.9 bits (1561), Expect = 3.4e-173
Identity = 293/463 (63.28%), Postives = 354/463 (76.46%), Query Frame = 0

Query: 123 VRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L + DP +  ++EKEK+RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF  D  +WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FES PYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DY RFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP ++        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGK 482
           LAVALKQ  T  +K Y +QVK NA AL + L+ K Y++VT GT+NH+VLWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQI 542
             E++C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I EFL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPG 586
            + +Q+ +GKL K F +GL +N ++ +L   V  FSA + MPG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Carg11313 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 595.1 bits (1533), Expect = 6.0e-170
Identity = 285/463 (61.56%), Postives = 345/463 (74.51%), Query Frame = 0

Query: 123 VRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+ PYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DY RFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP ++        E   YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGK 482
           LAVALKQV    +K Y +QVK NA  LAS L+ K Y LVT GTDNH++LWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQI 542
             E+VCE+C+ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I EFLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPG 586
            + +Q ++GK+ K F +GL +N E+ E+   V  F+  F MPG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Carg11313 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 479.2 bits (1232), Expect = 4.8e-135
Identity = 246/465 (52.90%), Postives = 317/465 (68.17%), Query Frame = 0

Query: 128 NQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARY 187
           N  L   DP +  I+E EK RQ KG+ELI SEN+   +VM+A+GS +TNKYSEG PGARY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 188 YCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLD 247
           Y GN+YID  ETLC++RAL AF LD + WGVNVQP S + ANF VYT LL P +RIM LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 248 TPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRPKILICG 307
            P GG+ SHGY T + +K+S  SIFFE+ PY+++  TGYIDYD++E+ A  FRPK+++ G
Sbjct: 172 LPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 308 GSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRG 367
            S+Y R  DY R R++ +K  AV++ DMA ISGLVAA    SPF Y DVVT+TTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 368 PRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHIAALAVAL 427
           PRG +IF+RKG K   +     QG E   YDFE+KIN AVFP LQGGPHN+ I  LAVAL
Sbjct: 292 PRGAMIFFRKGVKEINK-----QGKEV-LYDFEDKINQAVFPGLQGGPHNHTITGLAVAL 351

Query: 428 KQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTGKNFEQV 487
           KQ  T EYKAY +QV  N+   A  L+ + Y+LV+GGTDNH+VL +L+  G+ G   E+V
Sbjct: 352 KQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKV 411

Query: 488 CEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQIAIVVQ 547
            E  HI  NK T+ GD   + PGG+R+GTPA+TSRG +E DF  + E+   A  IA+ V+
Sbjct: 412 LEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK 471

Query: 548 RE-HGKLPKAFLRGLQSN----NEVVELGYRVASFSAKFAMPGLE 588
            E  G   K F+  ++S+    +E+ +L + V  F+ +F   G E
Sbjct: 472 SEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6576983.10.0e+00100.00Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023552257.10.0e+0099.66serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022922715.10.0e+0099.15serine hydroxymethyltransferase 7-like [Cucurbita moschata][more]
XP_022984574.10.0e+0097.28serine hydroxymethyltransferase 7-like [Cucurbita maxima][more]
XP_022149204.10.0e+0090.12serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 ser... [more]
Match NameE-valueIdentityDescription
Q84WV01.7e-23067.79Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM594.4e-22666.05Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
O232544.8e-17263.28Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM48.5e-16961.56Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q54Z268.0e-15157.39Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E9K30.0e+0099.15Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE... [more]
A0A6J1JAX60.0e+0097.28Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3... [more]
A0A6J1D7A80.0e+0090.12Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 P... [more]
A0A6J1FV710.0e+0089.78Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE... [more]
A0A6J1IXT50.0e+0089.61Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G36370.11.2e-23167.79serine hydroxymethyltransferase 7 [more]
AT1G22020.13.2e-22766.05serine hydroxymethyltransferase 6 [more]
AT4G13930.13.4e-17363.28serine hydroxymethyltransferase 4 [more]
AT4G13890.16.0e-17061.56Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G37930.14.8e-13552.90serine transhydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 135..584
e-value: 1.9E-191
score: 638.2
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 160..440
e-value: 1.9E-191
score: 638.2
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 132..535
e-value: 1.6E-169
score: 563.7
NoneNo IPR availablePIRSRPIRSR000412-50PIRSR000412-50coord: 130..586
e-value: 5.4E-165
score: 546.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11680:SF45SERINE HYDROXYMETHYLTRANSFERASEcoord: 74..587
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 74..587
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 130..585
score: 39.409615
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 135..543
e-value: 0.0
score: 569.142
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 355..371
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 121..585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11313-RACarg11313-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity