Carg11278 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11278
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome P450 87A3
Location	Carg_Chr14: 6051682 .. 6054764 (-)
RNA-Seq Expression	Carg11278
Synteny	Carg11278

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAAGATCTCACTCCAACAAAACGTCTAACCCTCTCTCTCTCTCTCTCCTTCGTTTCATAATAATCTTCAGCATATAGTTCTTCCCTATCCACCAAAACTTTTGATTAAATTCATAACCAAAATGTGGGTTCTTATGGCTATCATTTTTGGTACGAGTATCATAACCTGCCTCCATGCCTTGAAAAAATCCAAATATAATGCAAAACTTCCAAAAGGTTCAATGGGTTTCCCCATTCTTGGTGAAACAAACCACTTCTTTTCTCCCAATCCATCATTCGACGTATCTCCTTTCATCAAACACAGAGTTCTCAAGTACTCGAAACAAAAAAAGTTCATTTTTTTTCTTTGGAAATATCAATTTTCGCCCTTCAATTCCAATAAAATTTTGGTATTTTTGGTTCAGGTATGGACCCATTTTCAAAACAAGTTTAGTGGGGAAACCATTGATTATCTCAACAGATCCTGACCTAAACTATCTCATATTCGAACAAGAGGAAACATTGTTCGAATGTTGGTATCCAGAGACCTTCAAAAAGATATTTGGAGATCAATTTTTCGGATCCTTGCATGGCTTCATGCATAAGTATCTCAAGAACATGATTGTTAACGCTTTTGGAATTGAATGTTTGAAAAGTATGGTTTCTGAAGTTGAAGCTGCAGCAACTACAAGATTAAGAAGATGGGCATCTTGTAAAGTAGTTGAATTGAAGGATGAAATTGCAAATGTAAATTGATACTAAAAAATTTTCCTTTTTTTTTCTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTGTTCTTGATATTAATTTGAATCCTTGCAGATGATACTCGATCTCACGGCAAACAAGTTGATTAGTTATGATCCAGAAAATTCGCCAGAGAATTTGAGAGAGAATTTTGTTGCATTCATTCAGGGTTTGGTGTCTTTCCCTCTTGATATTCCTGGAACAGCTTATCATAAATGCTTACGGGTAATTTGAATTATTTTTTCATACTAAATTATTAAGTTAAATTCAATGATGTTTAATGGATTTGATTTGTTAAACAGGGTAGAAAAAGAGTGATGAGGATGCTGGACAACATGTTAGAAGAAAGGCAGCAAAATCCAAGGAAACAAAACGTTGATTTTTTTGACTTTGTTCTTCAAGAACTTCAAAAGGATGGAACACCTCTCACTAAAGAAATCGCTTTGGATTTGATCTTTTTGCTTCTTTTTGCTAGCTATGAAACCACTTCCATTGCTTTAACTTTAGCTATCAAATTTCTCCTAGATCATCCCCACGTCTTGAGCCAATTAACTGTGTGCTTCTTAAATTTTGACATCAATTTTGTTATTGGATTACCCACCATTGGATAGTCCCACATTCTTAACATTTTTTAATGTTTATTAGGAAGAACATGAGGGAATTTTGAAGAACAGGAAAGATAAAGCTTCTGGGATTACTTGGAATGAATACAAATCCATGACATTTACATTCAAGGTGAAGGAATATAGTAAAGTGGCTGGCATATGATGCACGTACAGGAATTACCATCCCGGCGGGATGGGGTGTCATGGTGTGTCCTCCAGCTATCCATTTGGATCCAAAGCATTATGTTGACCCACTTGCCTTCAACCCATGGCGGTGGGAGGTACGTTGAGCATATATTTTAACACCCTTTATATGTATACACACACACAATATGATGTGATACCTATAAATATTCATAAATATGTTATCCAACCCCGTCGTTCTAAAAATGTCGAAAGAAAAATTCGAACTCGAAAAGATGCAAAAAAAAAAAAAAAAGGCAACCCATTAATCTAATATTTCGAGGCAAAGAAAATGATTGTTTATTTGAGATATAATTCGACTGCTAATAAACTTGACTTAGTAGAATAAGTTACTTGCTATGAATCTATTTTAGCAAGTAATGTTAAGAGAGAATTTTACTAAACTACTAGCTAATAACAAGGTTATAATACTTATGGAGGCACATAGGAAAGCTAGAATGAGTGCTTTCTCGGCTATAAAATTTCATGAGATGTTAAAAAGCTAAGTGCTTAAGGTTCATTTGTTCATTTGCTTCTTGTTTCAACATATTTCGATCGTGGGTCACATCCAACACTGTTCACGAAAAAGATAGATCCAATCAAAATTGAATGATTTTAAATAAACATGGATAACTATTTAGAGTGTTAAAAACAAACGCTAGAGTACCGGTGGGGTCAAATTGAATAGTTGTAATTCTGGTGGATCGTAACCGAACATAGCCACCAAAATGTGAAGGAATTATTTAAACTTAATCCGAACAAGTTTATGATACGTATTTGTTGTAAGCAGAGATGTGAAGGGGCATCGAAGCATTTCATTGCTTTTGGAAGGGGCCTGAGATTTTGCATTGGTGCAGACTTTGCCAAATTGCAGATGGCTGTGTTTCTCCATAGCTTGGTTACCAAGTACAGGTACGTGTGTTCTAAATGCCTATGAAAATATGCATGAATACAAAACGAGGCTTTTTAATATTTTTGAATGCCACTTAGGTTCAAAGCAATTGGAGGAGGTAATATTGTTCGAACCCCAGGCGTACAGTTTCCTGATGGCTTTCACGTTGAGATCGTGGAGAAAGAGAAGAAGCCCTCCAAATACTAAACAAAACAAAAGAGTGTCATGTAATGCTTCTCAACAGTTAAAGACTATCCAAGTGGCAACTATGTGTTTTAAATCATATATTTATGATTAAACTATTTCGTAATAAAAACAAAACGTTGTGTGTCTCTCTTGTACACTTAGATGTATCGAGAATGGTGAGTTGTGAGCCTCAGTCAAAATCTCAAACTCATCAACTTTTCGTAAGCCTAAAATACTCTTGAAAAAAAGGATTGTGATCTAAGGACGGGGAAAACACATTCACCAACTTCACTCTAAACGACTCAAAAATGACTCAAAACTTTAATGAAGCTAGGATCTCTTTGTTGAAAATTTATGATACATAACTATCAATTGCGCCTACTAATATTATTATCTTATGCATGCTCCAAATCAATTTGTATCTAGATTTGTTCTGTGATTGAAGCAGATGTGAGGGCTATTTTTCT

mRNA sequence

ATTTAAGATCTCACTCCAACAAAACGTCTAACCCTCTCTCTCTCTCTCTCCTTCGTTTCATAATAATCTTCAGCATATAGTTCTTCCCTATCCACCAAAACTTTTGATTAAATTCATAACCAAAATGTGGGTTCTTATGGCTATCATTTTTGGTACGAGTATCATAACCTGCCTCCATGCCTTGAAAAAATCCAAATATAATGCAAAACTTCCAAAAGGTTCAATGGGTTTCCCCATTCTTGGTGAAACAAACCACTTCTTTTCTCCCAATCCATCATTCGACGTATCTCCTTTCATCAAACACAGAGTTCTCAAGTATGGACCCATTTTCAAAACAAGTTTAGTGGGGAAACCATTGATTATCTCAACAGATCCTGACCTAAACTATCTCATATTCGAACAAGAGGAAACATTGTTCGAATGTTGGTATCCAGAGACCTTCAAAAAGATATTTGGAGATCAATTTTTCGGATCCTTGCATGGCTTCATGCATAAGTATCTCAAGAACATGATTGTTAACGCTTTTGGAATTGAATGTTTGAAAAGTATGGTTTCTGAAGTTGAAGCTGCAGCAACTACAAGATTAAGAAGATGGGCATCTTGTAAAGTAGTTGAATTGAAGGATGAAATTGCAAATATGATACTCGATCTCACGGCAAACAAGTTGATTAGTTATGATCCAGAAAATTCGCCAGAGAATTTGAGAGAGAATTTTGTTGCATTCATTCAGGGTTTGGTGTCTTTCCCTCTTGATATTCCTGGAACAGCTTATCATAAATGCTTACGGGGTAGAAAAAGAGTGATGAGGATGCTGGACAACATGTTAGAAGAAAGGCAGCAAAATCCAAGGAAACAAAACGTTGATTTTTTTGACTTTGTTCTTCAAGAACTTCAAAAGGATGGAACACCTCTCACTAAAGAAATCGCTTTGGATTTGATCTTTTTGCTTCTTTTTGCTAGCTATGAAACCACTTCCATTGCTTTAACTTTAGCTATCAAATTTCTCCTAGATCATCCCCACGTCTTGAGCCAATTAACTGTGTGCTTCTTAAATTTTGACATCAATTTTGTTATTGGATTACCCACCATTGGATAGTCCCACATTCTTAACATTTTTTAATGTTTATTAGGAAGAACATGAGGGAATTTTGAAGAACAGGAAAGATAAAGCTTCTGGGATTACTTGGAATGAATACAAATCCATGACATTTACATTCAAGGAATTACCATCCCGGCGGGATGGGGTGTCATGGTGTGTCCTCCAGCTATCCATTTGGATCCAAAGCATTATGTTGACCCACTTGCCTTCAACCCATGGCGGTGGGAGAGATGTGAAGGGGCATCGAAGCATTTCATTGCTTTTGGAAGGGGCCTGAGATTTTGCATTGGTGCAGACTTTGCCAAATTGCAGATGGCTGTGTTTCTCCATAGCTTGGTTACCAAGTACAGGTTCAAAGCAATTGGAGGAGGTAATATTGTTCGAACCCCAGGCGTACAGTTTCCTGATGGCTTTCACGTTGAGATCGTGGAGAAAGAGAAGAAGCCCTCCAAATACTAAACAAAACAAAAGAGTGTCATGTAATGCTTCTCAACAGTTAAAGACTATCCAAGTGGCAACTATGTGTTTTAAATCATATATTTATGATTAAACTATTTCGTAATAAAAACAAAACGTTGTGTGTCTCTCTTGTACACTTAGATGTATCGAGAATGGTGAGTTGTGAGCCTCAGTCAAAATCTCAAACTCATCAACTTTTCGTAAGCCTAAAATACTCTTGAAAAAAAGGATTGTGATCTAAGGACGGGGAAAACACATTCACCAACTTCACTCTAAACGACTCAAAAATGACTCAAAACTTTAATGAAGCTAGGATCTCTTTGTTGAAAATTTATGATACATAACTATCAATTGCGCCTACTAATATTATTATCTTATGCATGCTCCAAATCAATTTGTATCTAGATTTGTTCTGTGATTGAAGCAGATGTGAGGGCTATTTTTCT

Coding sequence (CDS)

ATGTGGGTTCTTATGGCTATCATTTTTGGTACGAGTATCATAACCTGCCTCCATGCCTTGAAAAAATCCAAATATAATGCAAAACTTCCAAAAGGTTCAATGGGTTTCCCCATTCTTGGTGAAACAAACCACTTCTTTTCTCCCAATCCATCATTCGACGTATCTCCTTTCATCAAACACAGAGTTCTCAAGTATGGACCCATTTTCAAAACAAGTTTAGTGGGGAAACCATTGATTATCTCAACAGATCCTGACCTAAACTATCTCATATTCGAACAAGAGGAAACATTGTTCGAATGTTGGTATCCAGAGACCTTCAAAAAGATATTTGGAGATCAATTTTTCGGATCCTTGCATGGCTTCATGCATAAGTATCTCAAGAACATGATTGTTAACGCTTTTGGAATTGAATGTTTGAAAAGTATGGTTTCTGAAGTTGAAGCTGCAGCAACTACAAGATTAAGAAGATGGGCATCTTGTAAAGTAGTTGAATTGAAGGATGAAATTGCAAATATGATACTCGATCTCACGGCAAACAAGTTGATTAGTTATGATCCAGAAAATTCGCCAGAGAATTTGAGAGAGAATTTTGTTGCATTCATTCAGGGTTTGGTGTCTTTCCCTCTTGATATTCCTGGAACAGCTTATCATAAATGCTTACGGGGTAGAAAAAGAGTGATGAGGATGCTGGACAACATGTTAGAAGAAAGGCAGCAAAATCCAAGGAAACAAAACGTTGATTTTTTTGACTTTGTTCTTCAAGAACTTCAAAAGGATGGAACACCTCTCACTAAAGAAATCGCTTTGGATTTGATCTTTTTGCTTCTTTTTGCTAGCTATGAAACCACTTCCATTGCTTTAACTTTAGCTATCAAATTTCTCCTAGATCATCCCCACGTCTTGAGCCAATTAACTGTGTGCTTCTTAAATTTTGACATCAATTTTGTTATTGGATTACCCACCATTGGATAG

Protein sequence

MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHVLSQLTVCFLNFDINFVIGLPTIG

Homology

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BLAST of Carg11278 vs. NCBI nr
Match: KAG7018136.1 (Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 650.2 bits (1676), Expect = 9.3e-183
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLTVCFLNFDINFVIGLPTIG 324
           LSQLTVCFLNFDINFVIGLPTIG
Sbjct: 301 LSQLTVCFLNFDINFVIGLPTIG 323

[Close]

BLAST of Carg11278 vs. NCBI nr
Match: XP_022935244.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 599.4 bits (1544), Expect = 1.9e-167
Identity = 298/305 (97.70%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHAL KSKYNAKLPKGSMGFPILGETNHFF+PNPSFDVSPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALIKSKYNAKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKD 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIAN+ILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANVILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           L+QLT
Sbjct: 301 LTQLT 305

[Close]

BLAST of Carg11278 vs. NCBI nr
Match: XP_022983296.1 (cytochrome P450 87A3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 597.0 bits (1538), Expect = 9.4e-167
Identity = 296/305 (97.05%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHALK SKYN+KLPKGSMGFPILGETNHFF+PNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMV EVEAAA TRLRRWASC+VVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           LSQLT
Sbjct: 301 LSQLT 305

[Close]

BLAST of Carg11278 vs. NCBI nr
Match: XP_022983294.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 597.0 bits (1538), Expect = 9.4e-167
Identity = 296/305 (97.05%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHALK SKYN+KLPKGSMGFPILGETNHFF+PNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMV EVEAAA TRLRRWASC+VVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           LSQLT
Sbjct: 301 LSQLT 305

[Close]

BLAST of Carg11278 vs. NCBI nr
Match: KAG6581642.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545.4 bits (1404), Expect = 3.2e-151
Identity = 270/272 (99.26%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 34  MGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93
           MGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ
Sbjct: 1   MGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 60

Query: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTR 153
           EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKS+VSEVEAAATTR
Sbjct: 61  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSIVSEVEAAATTR 120

Query: 154 LRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213
           LRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG
Sbjct: 121 LRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 180

Query: 214 TAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIF 273
           TAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQEL+KDGTPLTKEIALDLIF
Sbjct: 181 TAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQELRKDGTPLTKEIALDLIF 240

Query: 274 LLLFASYETTSIALTLAIKFLLDHPHVLSQLT 306
           LLLFASYETTSIALTLAIKFLLDHPHVLSQLT
Sbjct: 241 LLLFASYETTSIALTLAIKFLLDHPHVLSQLT 272

[Close]

BLAST of Carg11278 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 319.7 bits (818), Expect = 3.8e-86
Identity = 154/281 (54.80%), Postives = 211/281 (75.09%), Query Frame = 0


Query: 26  NAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLIISTDPD 85
           N +LP GS+G P++GET  FF+PNP+ D+SPF+K R+ +YG IFKTS+VG+P+++S DP+
Sbjct: 68  NGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKERIKRYGSIFKTSVVGRPVVVSADPE 127

Query: 86  LNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKS-MVS 145
           +NY +F+QE  LFE WYP+TF +IFG    GSLHGFM+KYLK +++  +G E LKS +++
Sbjct: 128 MNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGFMYKYLKTLVLRLYGQENLKSVLLA 187

Query: 146 EVEAAATTRLRRWASCKVVELKDEIANMILDLTANKLISYDPEN-SPENLRENFVAFIQG 205
           E +AA    L  WAS   VELK+ I+ MI DLTA KLI YDP   S  NLR+NF AFI G
Sbjct: 188 ETDAACRGSLASWASQPSVELKEGISTMIFDLTAKKLIGYDPSKPSQVNLRKNFGAFICG 247

Query: 206 LVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQNPRKQNVDFFDFVLQELQKDGTPL 265
           L+SFPL+IPGTAYH+C+ GRK  M++L  M++ER   P +   DFFD V+QEL+++   L
Sbjct: 248 LISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERMAEPERPCEDFFDHVIQELRREKPLL 307

Query: 266 TKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHVLSQL 305
           T+ IALDL+F+LLFAS+ETT++ALT+ +K L ++P V+  L
Sbjct: 308 TETIALDLMFVLLFASFETTALALTIGVKLLTENPKVVDAL 348

[Close]

BLAST of Carg11278 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 5.7e-50
Identity = 108/309 (34.95%), Postives = 175/309 (56.63%), Query Frame = 0


Query: 1   MWVL---MAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPF 60
           MW +   +A +F    I  ++  + SK+N  LP G+MG P++GET     P+ S DV PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IKHRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFG-DQFFG 120
           I+ +V +YGPIFKT L G+P+++S D + N  I  QE    E WY +T  K FG D  + 
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 SLHGFMHKYLKNMIVNAFGIECLKS-MVSEVEAAATTRLRRWASCKVVELKDEIANMILD 180
              G +HKY++++ +N FG E L+   +  +EA++   L  W++   VE+K+  A M+  
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 LTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLE 240
            + NK+   D +    N+   F   + G +S PL+ PGT YHKCL+  K + + L  +++
Sbjct: 181 TSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVVD 240

Query: 241 ERQQNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLL 300
           +R  N      DF    L++ + +   +++E  + L+F + FAS+E+ S  LTL +K L 
Sbjct: 241 DRLANVGPDVEDFLGQALKDKESEKF-ISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 DHPHVLSQL 305
           +HP V+ +L
Sbjct: 301 EHPEVVKEL 308

[Close]

BLAST of Carg11278 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 162.5 bits (410), Expect = 7.7e-39
Identity = 98/306 (32.03%), Postives = 168/306 (54.90%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           +WV+   +    I   L+     K N KLP GSMG PI+GET  FF P+  +++SPF+K 
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           R+LKYGP+F+T++ G   ++ T+PD+ + +F QE   F   YPE F K FG +     HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIVNAFGIECL-KSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTAN 180
            +HK++K + +   G E L K M+ E++      LR  A+    + K+ + ++I+     
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 KLISYDPENSPENLRENFVAFIQGLVSFPLDIPG-TAYHKCLRGRKRVMRMLDNMLEERQ 240
           K+IS     +   L +N +A        PL +    + +K    R+  ++++ ++   R+
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 QNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHP 300
            + R+   DF D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++ 
Sbjct: 249 AS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENH 308

Query: 301 HVLSQL 305
             L++L
Sbjct: 309 KALAEL 311

[Close]

BLAST of Carg11278 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 103/310 (33.23%), Postives = 164/310 (52.90%), Query Frame = 0


Query: 21  KKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKHRVLKYGPIFKTSLVGKPLII 80
           +K+++N  LP G  G+P LGET  +  P  +  +  F++  V KYG I++++L G+P I+
Sbjct: 33  RKTRFN--LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIV 92

Query: 81  STDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLK 140
           S D  LN  I + E  LFEC YP +   I G      L G MH+ ++++ +N      L+
Sbjct: 93  SADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLR 152

Query: 141 S-MVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANKLISYDP-ENSPENLRENFV 200
           + ++ +VE      L  W    +   +DE      +L A  ++S DP E   E L++ +V
Sbjct: 153 TILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYV 212

Query: 201 AFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQ-------------------- 260
            F++G+VS PL++PGTAYHK L+ R  +++ ++  +EER+                    
Sbjct: 213 TFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAE 272

Query: 261 ----QNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFL 305
                + RKQ  D  D +L  + K     T++I LDLI  LLFA +ET+S+A+ LAI FL
Sbjct: 273 MSKSDHVRKQRTD--DDLLGWVLKHSNLSTEQI-LDLILSLLFAGHETSSVAIALAIFFL 332

[Close]

BLAST of Carg11278 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.0e-38
Identity = 100/317 (31.55%), Postives = 163/317 (51.42%), Query Frame = 0


Query: 4   LMAIIFGTSIITCLHAL---------KKSKYNAK-----LPKGSMGFPILGETNHFFSPN 63
           L+ ++F T+ +  LH +         +K   N K     LP GS G+P++GET  ++   
Sbjct: 7   LLLVVF-TAAVALLHLIYRWWNAQRGQKRTSNEKNQELHLPPGSTGWPLIGETYSYYRSM 66

Query: 64  PSFDVSPFIKHRVLKY-GPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKK 123
            S     FI  R  +Y   +F + L G   +IS+DP  N  + + E   F+  YP+  K 
Sbjct: 67  TSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQFNKYVLQNEGRFFQAHYPKALKA 126

Query: 124 IFGDQFFGSLHGFMHKYLKNMIVNAFGIECLK-SMVSEVEAAATTRLRRWASCKVVELKD 183
           + GD    S+HG + + L  + VN    E LK   + E++    + L RW   K + L++
Sbjct: 127 LIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEIQNLVHSTLDRWVDKKEIALQN 186

Query: 184 EIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVM 243
           E   M+L+L A +L+   P      + E FV +   +++ P+ IPG+ Y K L+ R+ ++
Sbjct: 187 ECHQMVLNLMAKQLLDLSPSKETNEICELFVDYTNAVIAIPIKIPGSTYAKGLKARELLI 246

Query: 244 RMLDNMLEERQQNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIAL 303
           R + NM++ER+ +P   + D    +L+E       ++ EI  D I  LLFA +ET+S A+
Sbjct: 247 RKISNMIKERRDHPHIVHKDLLTKLLEE-----DSISDEIICDFILFLLFAGHETSSRAM 306

Query: 304 TLAIKFLLDHPHVLSQL 305
           T AIKFL   P  L+Q+
Sbjct: 307 TFAIKFLTTCPKALTQM 317

[Close]

BLAST of Carg11278 vs. ExPASy TrEMBL
Match: A0A6J1F4V6 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442183 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.2e-168
Identity = 298/305 (97.70%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHAL KSKYNAKLPKGSMGFPILGETNHFF+PNPSFDVSPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALIKSKYNAKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKD 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIAN+ILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANVILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           L+QLT
Sbjct: 301 LTQLT 305

[Close]

BLAST of Carg11278 vs. ExPASy TrEMBL
Match: A0A6J1J1Q7 (cytochrome P450 87A3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.5e-167
Identity = 296/305 (97.05%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHALK SKYN+KLPKGSMGFPILGETNHFF+PNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMV EVEAAA TRLRRWASC+VVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           LSQLT
Sbjct: 301 LSQLT 305

[Close]

BLAST of Carg11278 vs. ExPASy TrEMBL
Match: A0A6J1J6X9 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.5e-167
Identity = 296/305 (97.05%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHALK SKYN+KLPKGSMGFPILGETNHFF+PNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMV EVEAAA TRLRRWASC+VVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV
Sbjct: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300

Query: 301 LSQLT 306
           LSQLT
Sbjct: 301 LSQLT 305

[Close]

BLAST of Carg11278 vs. ExPASy TrEMBL
Match: A0A6J1FA36 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442183 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.8e-135
Identity = 239/245 (97.55%), Postives = 242/245 (98.78%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHAL KSKYNAKLPKGSMGFPILGETNHFF+PNPSFDVSPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALIKSKYNAKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKD 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIAN+ILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANVILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQN 246
           PRKQN
Sbjct: 241 PRKQN 245

[Close]

BLAST of Carg11278 vs. ExPASy TrEMBL
Match: A0A6J1J5G8 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.6e-134
Identity = 236/245 (96.33%), Postives = 240/245 (97.96%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           MWVLMAIIFG SIITCLHALK SKYN+KLPKGSMGFPILGETNHFF+PNPSFDVSPFIKH
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FMHKYLKNMIVNAFGIECLKSMV EVEAAA TRLRRWASC+VVELKDEIANMILDLTANK
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRML+NMLEERQQN
Sbjct: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLENMLEERQQN 240

Query: 241 PRKQN 246
           PRKQN
Sbjct: 241 PRKQN 245

[Close]

BLAST of Carg11278 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 378.6 bits (971), Expect = 4.9e-105
Identity = 187/305 (61.31%), Postives = 236/305 (77.38%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           +WV + +I   SI   +++ +  K   KLP GSMGFP+LGE+  FF PN + D+ PFIK 
Sbjct: 6   IWVSLLLI---SITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFIKE 65

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           RV KYGPIFKT+LVG+P+I+STD DL+Y +F QE   F+ WYP+TF  IFG +  GSLHG
Sbjct: 66  RVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHG 125

Query: 121 FMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANK 180
           FM+KYLKNM++  FG + LK M+ +VE  A  RL  W++   VELKD  A+MI DLTA K
Sbjct: 126 FMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTAKK 185

Query: 181 LISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 240
           LIS+DP+ S ENLR NFVAFIQGL+SFP DIPGTAYHKCL+GR + M+ML NML+ER++N
Sbjct: 186 LISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERREN 245

Query: 241 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 300
           PRK   DFFD+V++E+QK+GT LT+EIALDL+F+LLFAS+ETTS+ALTLAIKFL D P V
Sbjct: 246 PRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDPEV 305

Query: 301 LSQLT 306
           L +LT
Sbjct: 306 LKRLT 307

[Close]

BLAST of Carg11278 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 371.3 bits (952), Expect = 7.8e-103
Identity = 186/311 (59.81%), Postives = 236/311 (75.88%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           +WV + +I   SI   +++ +  K   KLP GSMGFP+LGE+  FF PN + D+ PFIK 
Sbjct: 6   IWVSLLLI---SITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFIKE 65

Query: 61  RV------LKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQF 120
           RV       +YGPIFKT+LVG+P+I+STD DL+Y +F QE   F+ WYP+TF  IFG + 
Sbjct: 66  RVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKN 125

Query: 121 FGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTRLRRWASCKVVELKDEIANMIL 180
            GSLHGFM+KYLKNM++  FG + LK M+ +VE  A  RL  W++   VELKD  A+MI 
Sbjct: 126 VGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRLELWSNQDSVELKDATASMIF 185

Query: 181 DLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNML 240
           DLTA KLIS+DP+ S ENLR NFVAFIQGL+SFP DIPGTAYHKCL+GR + M+ML NML
Sbjct: 186 DLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNML 245

Query: 241 EERQQNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFL 300
           +ER++NPRK   DFFD+V++E+QK+GT LT+EIALDL+F+LLFAS+ETTS+ALTLAIKFL
Sbjct: 246 QERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFL 305

Query: 301 LDHPHVLSQLT 306
            D P VL +LT
Sbjct: 306 SDDPEVLKRLT 313

[Close]

BLAST of Carg11278 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 176.4 bits (446), Expect = 3.7e-44
Identity = 109/304 (35.86%), Postives = 168/304 (55.26%), Query Frame = 0


Query: 3   VLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKHRV 62
           +LM  +    I   L+     K   KLP GSMGFPI+GET  FF P     +  F+K R+
Sbjct: 9   MLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTFVKKRM 68

Query: 63  LKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHGFM 122
           ++YGP+F+T++ G   ++STDPD+ + IF QE T FE  YP+ F K+FG         F+
Sbjct: 69  IRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEVFI 128

Query: 123 HKYLKNMIVNAFGIECLK-SMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANKL 182
           HKYL+ + +   G E LK +M+  ++ A    +R  AS     ++ E+ N+++     KL
Sbjct: 129 HKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPKL 188

Query: 183 ISYDPENSPENLRENFVAF-IQGLVSFPLDIPGTAYHKCLRGRKRVMRMLDNMLEERQQN 242
           IS     +   L +N  AF +    SF       A  K L+ R+  ++++ ++L  R++ 
Sbjct: 189 ISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRKET 248

Query: 243 PRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHV 302
             KQ  DF + +L+EL+KDG+   +  A++LIFLL FA  E TS    LA+KF+   P V
Sbjct: 249 REKQE-DFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDPKV 308

Query: 303 LSQL 305
           L++L
Sbjct: 309 LAEL 311

[Close]

BLAST of Carg11278 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 164.1 bits (414), Expect = 1.9e-40
Identity = 100/302 (33.11%), Postives = 163/302 (53.97%), Query Frame = 0


Query: 26  NAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKHRVLKY-GPIFKTSLVGKPLIISTDP 85
           + KLP GSMGFP++GET  FF P    ++ PF+K R+ K+ G +F+T+++G   I+STDP
Sbjct: 177 SGKLPPGSMGFPVIGETVEFFKPYSFNEIHPFVKKRMFKHGGSLFRTNILGSKTIVSTDP 236

Query: 86  DLNYLIFEQEETLFECWYPETFKKIFG-DQFFGSLHGFMHKYLKNMIVNAFGIECLKS-M 145
           ++N+ I +QE   F   YPE   +IFG D  F       H+Y++++ +   G ECLK   
Sbjct: 237 EVNFEILKQENRCFIMSYPEALVRIFGKDNLFFKQGKDFHRYMRHIALQLLGPECLKQRF 296

Query: 146 VSEVEAAATTRLRRWASCKVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQ 205
           + +++ A +  L+  +   VV++KD    +IL+     +IS     +   L E+F  F  
Sbjct: 297 IQQIDIATSEHLKSVSFQGVVDVKDTSGRLILEQMILMIISNIKPETKSKLIESFRDFSF 356

Query: 206 GLVSFPLD--IPGTAYHKCL---------------RGRKRVMRMLDNMLEERQQNPRKQN 265
            LV  P D       Y+  +               + R  VM+ML  M +ER++     +
Sbjct: 357 DLVRSPFDPSFWNALYNGLMDFVWLLNILDPLFYVKARSNVMKMLKRMFKERREEATSDD 416

Query: 266 V---DFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHPHVLS 305
               DF + ++ E++K+G  + +E +++LI  LL ASYETTS    L +KF+ ++P VL 
Sbjct: 417 SKYGDFMETMIYEVEKEGDTINEERSVELILSLLIASYETTSTMTALTVKFIAENPKVLM 476

[Close]

BLAST of Carg11278 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 162.5 bits (410), Expect = 5.5e-40
Identity = 98/306 (32.03%), Postives = 168/306 (54.90%), Query Frame = 0


Query: 1   MWVLMAIIFGTSIITCLHALKKSKYNAKLPKGSMGFPILGETNHFFSPNPSFDVSPFIKH 60
           +WV+   +    I   L+     K N KLP GSMG PI+GET  FF P+  +++SPF+K 
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120
           R+LKYGP+F+T++ G   ++ T+PD+ + +F QE   F   YPE F K FG +     HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIVNAFGIECL-KSMVSEVEAAATTRLRRWASCKVVELKDEIANMILDLTAN 180
            +HK++K + +   G E L K M+ E++      LR  A+    + K+ + ++I+     
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 KLISYDPENSPENLRENFVAFIQGLVSFPLDIPG-TAYHKCLRGRKRVMRMLDNMLEERQ 240
           K+IS     +   L +N +A        PL +    + +K    R+  ++++ ++   R+
Sbjct: 189 KIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRK 248

Query: 241 QNPRKQNVDFFDFVLQELQKDGTPLTKEIALDLIFLLLFASYETTSIALTLAIKFLLDHP 300
            + R+   DF D +++E +K+     +E A++LIF +L  + E+TS   +LAIKFL ++ 
Sbjct: 249 AS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENH 308

Query: 301 HVLSQL 305
             L++L
Sbjct: 309 KALAEL 311

The following BLAST results are available for this feature:

BLAST of Carg11278 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018136.1	9.3e-183	100.00	Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022935244.1	1.9e-167	97.70	cytochrome P450 87A3-like isoform X1 [Cucurbita moschata]	[more]
XP_022983296.1	9.4e-167	97.05	cytochrome P450 87A3-like isoform X3 [Cucurbita maxima]	[more]
XP_022983294.1	9.4e-167	97.05	cytochrome P450 87A3-like isoform X1 [Cucurbita maxima]	[more]
KAG6581642.1	3.2e-151	99.26	Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg11278 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XU38	3.8e-86	54.80	Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV...	[more]
K7NBR2	5.7e-50	34.95	Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=...	[more]
Q8L7D5	7.7e-39	32.03	Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3	[more]
O64989	1.3e-38	33.23	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]
Q50EK6	5.0e-38	31.55	Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1	[more]

BLAST of Carg11278 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F4V6	9.2e-168	97.70	cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114421...	[more]
A0A6J1J1Q7	4.5e-167	97.05	cytochrome P450 87A3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481917...	[more]
A0A6J1J6X9	4.5e-167	97.05	cytochrome P450 87A3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481917...	[more]
A0A6J1FA36	7.8e-135	97.55	cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114421...	[more]
A0A6J1J5G8	8.6e-134	96.33	cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481917...	[more]

BLAST of Carg11278 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12740.1	4.9e-105	61.31	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	7.8e-103	59.81	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G78490.1	3.7e-44	35.86	cytochrome P450, family 708, subfamily A, polypeptide 3	[more]
AT1G55940.1	1.9e-40	33.11	cytochrome P450, family 708, subfamily A, polypeptide 1	[more]
AT5G48000.2	5.5e-40	32.03	cytochrome P450, family 708, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..306 e-value: 1.7E-60 score: 207.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 26..304
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 64..304 e-value: 5.3E-16 score: 58.4
None	No IPR available	PANTHER	PTHR24286:SF11	CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2	coord: 12..306
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 12..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11278-RA	Carg11278-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen