Carg11199 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11199
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	structure-specific endonuclease subunit slx1
Location	Carg_Chr14: 5288301 .. 5291036 (+)
RNA-Seq Expression	Carg11199
Synteny	Carg11199

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCCCAGAAGTTTCGAAATCTTCTTCTTCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGGTATGTCGAATTTCTTCTCTTACGTTTCTTGTTTGAATCCCAGATTCCATGATTTGGTGTTGAAATTTTTTCTAGGTCGTGTTCGATTTAGCCTTAAAAATTGATATTTCGAGCTTTTAGGTTAAACATTTGGCTTCATATTGTTGAATAATCTGACTCGATCAAACTCACACACCGATGAACCAGAAATTAAATAAAGAGACACAAAGATTTACGTCATTCATCCACGGACTGCCACCAATCACAATAATCAAAGTGTATATACAAAGTCTCACTTCCTCTCAAATGCCACTAGGTAAATACAAAATGACACTCAATCTCAATAACCATCCTAGAAGATCATTTATATAAGCATACAAGATGATTCATGTATCAATATGAGAGATGAACCATATGAATTCCAGTAGGTGTATGCATAGGAGAAGGGAATAGGAGAAGGGAATGTACCAAAAATGTTCATGAATCCGATTCTTTATGTCAATTTACAACCGTTACTAGGTGAATAAGTGCAATTCTTTGAAAAATATGCTCAAATTGTGTCACTTGTTATGGATATAACTTTGTATTGAGTCAATTTGGTGCATAAAGACGAGGTTGGTCTTTGAAAAAGAACTTGGGTTTAGGTGCTGAACTGCTTGATTAATCAGTCGAGGTATTGATTTTCTTTTGAGCTTGTAAGATAGGGAGTGTTTTGGGATCATGCCCACACTGTTGTGAGATATGCTTTACGCATAAGCGGTATTCAAATATTTCTTAATGTCACGAAGTTGTATTCTTGTACAGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGGTATCCATTTAAAATAGAATTTCTAGGGCATTGCCTGCCTATTTGTGATTGTAGTCATTCTAGACATTATGTTGCTTAGGGCTCTTGAACAGGTGAGATATTTCCTAGTTTCTTTGTTCTGGTAGTGATCTTCCTAAGTTAGATGTAACAGTCCAAGCCACCGCTAGCACTTCTAGCAGATATTGTTCTCTTTGAACTTTTCTTTTCGGACCAGAACCTTAACCTGACGCCTTTGACCAACTCGGCATCTAATGGTATCAGAGCCAGACATCGAGCGGTGTGCCAGCGAGGACGCTGACCCCCAAGGGGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGAAAAATTCTCCCTAGCAAACGCGTTTTTAAAACTATGAGGCTTACGACGATATGTAACAGGACAAAGTGGACAATATTTGCTAGTAGTGGACTTGAGCTGTTACATTAGATGTCAATGGTCAGATGTATTTCAGACTTTCTCGTATTGTGAATACTATTTCCGTGAGAAACAATCTTTATTCATTGCTCCACGCTGATATTTATTATAAGATGCATTTTAATCATCATGGATTGATTTAGTTGTGACCAAAGACCATGTAAATAAATGAAGAACTTGAAGAGAATGAGTTCAAATTATGGTGCCGATCCATGTAGAATATTGTACAAGTTACCTAGGCATCCAAATGTCGTAGGGCGAGATTGTTGTCGTGTGAGAATAGCTGAGGTGTGCATAAGTTGTGCTAGTGTGGTAATCTCCCGCTCCCAAATTATATGCATGTTAGGACTCATACCTTCCAAATGGGGCCTTCCATCTTTAGTGTATTTTGATTTGATTTTTTCCCCTGGAAATGAAACAAGCTGCAACTTATAGCATTTCTGATTTAAATAGCAGCTTGAAAGCCCCCTTGTTTTTTAGTTTTTTTATTATTTTTTTTTTATTTATCAAACTGATAAAGGAAAGAAGGTGGGTCGTTAAGGCCAAGGCAAGGGGCAGCTCCCTTGTTGAAGTAGTATCAAGGCAAGGGGCGTCAGTAATGGCCCCACGCGTAGGCGTCTCGACTTGTTGTTATGAACATTCTTCAAGCTCATATCTCAAGAGCCTGTTTTGACCGGCTTTCTAAGAGCTTAAAAATATGATTGGTTTGACTTAAAACACATCATTCTAAACATGTTTTGCAGTTCTATTTGGACCACCAACCCGATAATCAAATTTCTCTAGAGCTTTCGAACCGAGAAATGTTGGATAAAATCATTCCCACACCTTTACTGATGCTGTTTTCGTGTACTTTGGTATGCAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATTTGAGAAAGGAAGAAGTAGATGATGTGACATTAAGGTTGCTGAAGCAAAGGCAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCCTCCTGATTCTTTTACAATATTTATCAATTTATTACCAAAAGTTGTTTATTAGATAAAATGCTAAATTAATTAAAATCTCCTTAAACTTTACTTTTCATAAATAAATAAAATATTTTTAAATCTTCATAAGTTTCTTC

mRNA sequence

ATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCCCAGAAGTTTCGAAATCTTCTTCTTCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATTTGAGAAAGGAAGAAGTAGATGATGTGACATTAAGGTTGCTGAAGCAAAGGCAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCCTCCTGATTCTTTTACAATATTTATCAATTTATTACCAAAAGTTGTTTATTAGATAAAATGCTAAATTAATTAAAATCTCCTTAAACTTTACTTTTCATAAATAAATAAAATATTTTTAAATCTTCATAAGTTTCTTC

Coding sequence (CDS)

ATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCCCAGAAGTTTCGAAATCTTCTTCTTCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATTTGAGAAAGGAAGAAGTAGATGATGTGACATTAAGGTTGCTGAAGCAAAGGCAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCCTCCTGA

Protein sequence

MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMCHLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS

Homology

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BLAST of Carg11199 vs. NCBI nr
Match: KAG7018060.1 (slx1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.0 bits (863), Expect = 8.8e-89
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC
Sbjct: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 164
           HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS
Sbjct: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 163

[Close]

BLAST of Carg11199 vs. NCBI nr
Match: KAG6581557.1 (hypothetical protein SDJN03_21559, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.0 bits (863), Expect = 8.8e-89
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV
Sbjct: 2   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 61

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC
Sbjct: 62  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 121

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 164
           HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS
Sbjct: 122 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 164

[Close]

BLAST of Carg11199 vs. NCBI nr
Match: XP_022934184.1 (structure-specific endonuclease subunit slx1 [Cucurbita moschata])

HSP 1 Score: 336.3 bits (861), Expect = 1.5e-88
Identity = 162/163 (99.39%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC
Sbjct: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 164
           H+RKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS
Sbjct: 121 HMRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 163

[Close]

BLAST of Carg11199 vs. NCBI nr
Match: XP_023528241.1 (structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.3 bits (830), Expect = 5.9e-85
Identity = 159/163 (97.55%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 60
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY
Sbjct: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61

Query: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 120
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM
Sbjct: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121

Query: 121 CHLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
           CH+RKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 164

[Close]

BLAST of Carg11199 vs. NCBI nr
Match: XP_022982643.1 (structure-specific endonuclease subunit slx1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 320.9 bits (821), Expect = 6.5e-84
Identity = 155/162 (95.68%), Postives = 158/162 (97.53%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTL +KSEPE KKWCVYLIISSNPPIKTYV
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLTIKSEPETKKWCVYLIISSNPPIKTYV 60

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFK+QSQACEFESKWKEICRKM 
Sbjct: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICRKMS 120

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
           H+RKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 HMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Carg11199 vs. ExPASy Swiss-Prot
Match: B6JY16 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=slx1 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.3e-13
Identity = 34/71 (47.89%), Postives = 46/71 (64.79%), Query Frame = 0


Query: 44  WCVYLIISSNPPIKT-YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQ 103
           +C YL++S     ++ Y+G T D  RRL+QHNGEIKGGA  T+  RPW  AC +HGF  +
Sbjct: 3   YCCYLLVSEKAASRSVYIGSTPDPARRLRQHNGEIKGGAYKTKRSRPWKVACFVHGFPTK 62

Query: 104 SQACEFESKWK 114
             A +FE  W+
Sbjct: 63  IAALQFEWVWQ 73

[Close]

BLAST of Carg11199 vs. ExPASy Swiss-Prot
Match: Q9P7M3 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=slx1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.9e-12
Identity = 32/74 (43.24%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 44  WCVYLIISSNPPIK--TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKD 103
           +C YL+ S+        Y+G T D  RRL+QHNGEI GGA  T+ GRPW  +C ++GF +
Sbjct: 7   YCCYLLKSNRTQSSGAVYIGSTPDPPRRLRQHNGEIVGGASKTKHGRPWSISCLVYGFPN 66

Query: 104 QSQACEFESKWKEI 116
           +  A +FE  W+ +
Sbjct: 67  KVSALKFEWNWQNL 80

[Close]

BLAST of Carg11199 vs. ExPASy Swiss-Prot
Match: B8MDD1 (Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=slx1 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-11
Identity = 43/128 (33.59%), Postives = 63/128 (49.22%), Query Frame = 0


Query: 27  STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRA 86
           +T V    V ++P P  +C YL+ S+  P   Y+G T D  RRL+QHNG  KGGAK T  
Sbjct: 2   TTTVSEEAVLTKPIPAFYCCYLLRSAKRPSALYIGSTPDPARRLEQHNGFAKGGAKRTER 61

Query: 87  G--RPWICACKIHGFKDQSQACEFESKWKEICRKMCHLRKEEVDDVTLRLLKQRQRALGK 146
              RPW     + GF  ++ A +FE  W+ +     H+   E D +     K++     K
Sbjct: 62  NTLRPWEMVAIVEGFPSRTGALQFEWSWQHV-HTTRHIGAVETDQLN---RKRKNPPTDK 121

Query: 147 VKGLFDCT 153
             G++  T
Sbjct: 122 GSGIWTST 125

[Close]

BLAST of Carg11199 vs. ExPASy Swiss-Prot
Match: B6QFH5 (Structure-specific endonuclease subunit slx1 OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=slx1 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-10
Identity = 39/105 (37.14%), Postives = 52/105 (49.52%), Query Frame = 0


Query: 31  PTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAG--R 90
           P  +   +P P  +C YL+ S   P   Y+G T D  RRL+QHNG  KGGAK T     R
Sbjct: 6   PADLPSPKPIPAFYCCYLLRSVKKPSSLYIGSTPDPARRLEQHNGFTKGGAKRTERDTLR 65

Query: 91  PWICACKIHGFKDQSQACEFESKWKEICRKMCHLRKEEVDDVTLR 134
           PW     I GF  ++ A +FE  W+ +     H+   E D +  R
Sbjct: 66  PWEMITIIEGFTSRTGALQFEWSWQHV-HTTRHIGAVETDQLNRR 109

[Close]

BLAST of Carg11199 vs. ExPASy Swiss-Prot
Match: Q4D7L5 (Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053509453.60 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 45  CVYLIISSNPPI--KTYVGVTLDFDRRLKQHNGEIKGGA-KATRAGRPWICACKIHGFKD 104
           CVYL+ S +P    + Y+G T+D  RRL+QHNGEI  GA +  R GRPW   C + GF +
Sbjct: 7   CVYLLTSLDPQCAGEYYIGYTVDPIRRLRQHNGEIVSGAWRTKRRGRPWELLCCVSGFGE 66

Query: 105 QSQACEFESKWK 114
              A +FE  W+
Sbjct: 67  DRIALKFEWCWQ 78

[Close]

BLAST of Carg11199 vs. ExPASy TrEMBL
Match: A0A6J1F1V2 (structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LOC111441428 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 7.3e-89
Identity = 162/163 (99.39%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC
Sbjct: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 164
           H+RKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS
Sbjct: 121 HMRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPS 163

[Close]

BLAST of Carg11199 vs. ExPASy TrEMBL
Match: A0A6J1J5C9 (structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481456 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.2e-84
Identity = 155/162 (95.68%), Postives = 158/162 (97.53%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYV 60
           MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTL +KSEPE KKWCVYLIISSNPPIKTYV
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLTIKSEPETKKWCVYLIISSNPPIKTYV 60

Query: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMC 120
           GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFK+QSQACEFESKWKEICRKM 
Sbjct: 61  GVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICRKMS 120

Query: 121 HLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
           H+RKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 HMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Carg11199 vs. ExPASy TrEMBL
Match: A0A0A0LCZ9 (GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.4e-68
Identity = 134/169 (79.29%), Postives = 148/169 (87.57%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVKSEPEP--KKWCVYLIISSNPPIKT 60
           MRLLS TFR  K PIS+P +SKSSSSS+K PTLIVKSEP+P  K+WCVYLIISSN PIKT
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSSKDPTLIVKSEPKPKLKQWCVYLIISSNSPIKT 60

Query: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 120
           YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICAC IHGFKDQSQACEFESKWK++ RK
Sbjct: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKVSRK 120

Query: 121 MCHLRKEE-----VDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
           + + +KEE     +DD TLRLLK R+RALGKVK LFDC+QFEFDW+LDP
Sbjct: 121 ISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDP 169

[Close]

BLAST of Carg11199 vs. ExPASy TrEMBL
Match: A0A1S3CFH5 (structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC103500344 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.1e-67
Identity = 132/168 (78.57%), Postives = 147/168 (87.50%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVPTLIVK-SEPEPKKWCVYLIISSNPPIKTY 60
           MRLLSQTFR QK PIS+P + KSSSSSTK PTLIVK  EP+PK+WCVYLIISSN PIKTY
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSSSTKGPTLIVKLEEPKPKQWCVYLIISSNSPIKTY 60

Query: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 120
           VGVTLDFDRRLKQHNGEIKGGAKAT+AGRPWICAC IHGFKDQSQACEFESKWK++ RK+
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSRKI 120

Query: 121 CHLRKE-----EVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
            + +KE     ++D+ TLRLLK RQRALGKVK LFDC+ FEFDW+LDP
Sbjct: 121 SYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDP 168

[Close]

BLAST of Carg11199 vs. ExPASy TrEMBL
Match: A0A6J1DGL6 (structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=LOC111020266 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.3e-58
Identity = 121/170 (71.18%), Postives = 136/170 (80.00%), Query Frame = 0


Query: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVP---TLIVKSEPEPKKWCVYLIISSNPPIK 60
           MRLLS TFR  K PIS+P+   SSSSSTK P   TL  +S+ +PK WCVYLIISSN P K
Sbjct: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92

Query: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICR 120
           TYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ R
Sbjct: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152

Query: 121 KMCHLRK-----EEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163
           K+ H RK     E+ D+  LRLLK RQRAL KVK +FDC++FEFD +LDP
Sbjct: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDP 200

[Close]

BLAST of Carg11199 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 157.5 bits (397), Expect = 8.9e-39
Identity = 85/168 (50.60%), Postives = 109/168 (64.88%), Query Frame = 0


Query: 2   RLLSQTFRSQKLPISSPEVS-----KSSSSSTKVPTLIVKSE----PEPKKWCVYLIISS 61
           RLLS+TF S KL  S+P+       K+S +   +P+     E    P+ K W VYLI+S+
Sbjct: 3   RLLSRTFPSVKLRDSNPKAPEFKNLKNSQTQVPIPSSSSPQENSKIPKSKSWSVYLILST 62

Query: 62  NPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKW 121
             PIKTYVG+T DF RRLKQHNGEI+GGAKA+ AGRPW+CAC I GF   SQA  FESKW
Sbjct: 63  TEPIKTYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTCLSQASSFESKW 122

Query: 122 KEICRKMCHLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWEL 161
           K   RK+   +K+E    +  LL+ R+RAL KV+   DC+  E DW++
Sbjct: 123 KIFTRKLPRRKKDEDMSQSDALLQHRRRALNKVEESLDCSYLETDWKI 170

[Close]

BLAST of Carg11199 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 30/69 (43.48%), Postives = 41/69 (59.42%), Query Frame = 0


Query: 47  YLIISSNPPIK--TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQ 106
           YL+ S +P  K  TY+G T++  RR++QHNGEI  GA  T+  RPW     I+GF     
Sbjct: 31  YLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGFPTNVS 90

Query: 107 ACEFESKWK 114
           A +FE  W+
Sbjct: 91  ALQFEWAWQ 99

The following BLAST results are available for this feature:

BLAST of Carg11199 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018060.1	8.8e-89	100.00	slx1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581557.1	8.8e-89	100.00	hypothetical protein SDJN03_21559, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022934184.1	1.5e-88	99.39	structure-specific endonuclease subunit slx1 [Cucurbita moschata]	[more]
XP_023528241.1	5.9e-85	97.55	structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo]	[more]
XP_022982643.1	6.5e-84	95.68	structure-specific endonuclease subunit slx1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg11199 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6JY16	6.3e-13	47.89	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (s...	[more]
Q9P7M3	6.9e-12	43.24	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strai...	[more]
B8MDD1	3.4e-11	33.59	Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain A...	[more]
B6QFH5	1.7e-10	37.14	Structure-specific endonuclease subunit slx1 OS=Talaromyces marneffei (strain AT...	[more]
Q4D7L5	1.1e-09	45.83	Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (str...	[more]

BLAST of Carg11199 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1V2	7.3e-89	99.39	structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J5C9	3.2e-84	95.68	structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A0A0LCZ9	5.4e-68	79.29	GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=...	[more]
A0A1S3CFH5	2.1e-67	78.57	structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A6J1DGL6	2.3e-58	71.18	structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Carg11199 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43210.1	8.9e-39	50.60	Excinuclease ABC, C subunit, N-terminal	[more]
AT2G30350.2	1.1e-09	43.48	Excinuclease ABC, C subunit, N-terminal	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035901	GIY-YIG endonuclease superfamily	GENE3D	3.40.1440.10		coord: 38..141 e-value: 3.6E-23 score: 83.4
IPR035901	GIY-YIG endonuclease superfamily	SUPERFAMILY	82771	GIY-YIG endonuclease	coord: 43..119
IPR000305	GIY-YIG endonuclease	PFAM	PF01541	GIY-YIG	coord: 43..114 e-value: 4.9E-14 score: 52.4
IPR000305	GIY-YIG endonuclease	PROSITE	PS50164	GIY_YIG	coord: 42..127 score: 17.163193
None	No IPR available	PANTHER	PTHR20208:SF13	EMB\|CAB76036.1	coord: 18..162
None	No IPR available	PANTHER	PTHR20208	STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1	coord: 18..162
None	No IPR available	CDD	cd10455	GIY-YIG_SLX1	coord: 44..113 e-value: 3.70338E-35 score: 115.793

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11199-RA	Carg11199-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0033557	Slx1-Slx4 complex
molecular_function	GO:0017108	5'-flap endonuclease activity
molecular_function	GO:0008821	crossover junction endodeoxyribonuclease activity