Carg11178 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11178
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	polygalacturonase At1g48100-like
Location	Carg_Chr14: 5099446 .. 5101675 (-)
RNA-Seq Expression	Carg11178
Synteny	Carg11178

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCCCCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCTCCTCCTCTTTCTCCTTCCCCCGAACCCACGAATCCTCCCGACGACGACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGATGACACGGAGGCCTTCAAGATGGCATGGGACACAGCCTGTCAAAGCGACAATGACGACTATGCAGTTATTCTTGTTCCATATGGCTTCTCGTTTATGATCCAATCGACAATTTTCACCGGGCCGTGCAAACACGGCGTCGTGTTTCAAGTTGATGGCACTCTCATAGCTCCGGATGGACCCGATGCTTGGCCGAAGAGTTACAGTGCTCATCAATGGCTGGTTTTTTATCGAGTTAATAACATGTCGCTTCGAGGAAATGGCGTTATTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGTAAGAGACATGACATTGATACTATGATGTTTTTTGAACATAACTATGAGCTTCTAATTATATTCCAATTTTCAGGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGTAAGTTAATTCAAGTCAAATTCTACAAAATGTTGTTGTTTATGAGCTTAGAATATGAACAATTGAAAGGGTTTTTGCAGGCCATAAGATTCTTCATGAGCTCAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGGTTCGACAATTGTAGAGATGTTTATATTGATTCTCTTACCATAACAGCTCCTGCTTTGAGTCCAAACACCGATGGAATTCACATTGAGAATACAAATGGGGTTCAGATACTCAATTCAGTTGTTGCCAATGGTTAGTTATGATCAACAACATTATTTCTTGTCATTTTCTTTGCTTTTTTCCACCAAAAATGTAAATTTGGGTTGAATTTTTTCTTACCCAGGTGATGATTGTATTTCAATTGGATCGGGTTCTTATAACGTTGATATACGAAATATTACGTGTGGACCAGGTCATGGAATTAGGTAACCGAAAGCTTATTATAATTCTATCCTCTATTCATTACAAGAAATAATAGATATGATAGCATACAAAAAATGCTATTTTAGACTTTTATAACGTTTTTTTTGTATGTTGTGACAGTCTATGTCATTAAAAGTCTGAGACTTTTTATGTGAGATTTTATATTGGTTGGAGAGGAGAATGAACCATTTTTCCCTAAGTTTTAAAAACCGGGAAGCGCGAAAGGGAAAACCCAAATAGGACAATATTTGCTAGTGGTGCGCTTGAACTGTTACAAATGGTATCAGAGCTAGACATCGGACGATGTGCCAGCGAGGACGCTGGCCCCCAAGGGGGGTGGATTGTGAGATCCCATGTTGGATGGAGATGAGAACGAAGCATTCTTTAGAAATGTGCGGAAACCTCTCCTTAACATACGCGTTGTAAACCCTTGAGGAGAAGCCCATAAAAAAAAGTTTGAAGAGGATAATATCTACTAGTGGAGCGTTTTTTTTTATAGTTATAATAGATTATAAAACTACTTGTTCGTCCTTTGATCTTTTCCATCTCCAGCATTGGCAGCCTAGGCAATCACAACTCCCGAGCATGCGTGTCGAACATAACAGTTCGAGACTCAATAATCAAGGTCTCAGACAACGGTGTTCGGATCAAGACATGGCAAGGTGGGTTTGGAGCGGTAAGAGGAGTAAGTTTCAATAACATTCATATGGACAACGTTAGAAACCCAATCATCATAGACCAATTCTATTGCCTCAACAAAGGATGTCTCAATCAAACCTCAGCCGTGGTTGTATCAGACATAAGCTATATTGACATCAAAGGAAGTTATGATATTAGAAGCCCACCAATGCACTTTGGGTGCAGTGATTCTATGCCTTGTGTGAACCTAACACTTGCAAATATCGAGCTTTTTCCTTCAAAAGGTGACATCTTTTTGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCCAGGTCTATCTTGGAGACTCAAAGGGATTATTGCTAA

mRNA sequence

ATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCCCCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCTCCTCCTCTTTCTCCTTCCCCCGAACCCACGAATCCTCCCGACGACGACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGATGACACGGAGGCCTTCAAGATGGCATGGGACACAGCCTTTAATAACATGTCGCTTCGAGGAAATGGCGTTATTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGCCATAAGATTCTTCATGAGCTCAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGTGATTCTATGCCTTGTGTGAACCTAACACTTGCAAATATCGAGCTTTTTCCTTCAAAAGGTGACATCTTTTTGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCCAGGTCTATCTTGGAGACTCAAAGGGATTATTGCTAA

Coding sequence (CDS)

ATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCCCCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCTCCTCCTCTTTCTCCTTCCCCCGAACCCACGAATCCTCCCGACGACGACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGATGACACGGAGGCCTTCAAGATGGCATGGGACACAGCCTTTAATAACATGTCGCTTCGAGGAAATGGCGTTATTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGCCATAAGATTCTTCATGAGCTCAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGTGATTCTATGCCTTGTGTGAACCTAACACTTGCAAATATCGAGCTTTTTCCTTCAAAAGGTGACATCTTTTTGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCCAGGTCTATCTTGGAGACTCAAAGGGATTATTGCTAA

Protein sequence

MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFSDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC

Homology

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BLAST of Carg11178 vs. NCBI nr
Match: KAG7018039.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 507.3 bits (1305), Expect = 7.2e-140
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60
           MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE
Sbjct: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60

Query: 61  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFNNMSLRGNGVIDG 120
           PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFNNMSLRGNGVIDG
Sbjct: 61  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFNNMSLRGNGVIDG 120

Query: 121 RGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFSDSMPC 180
           RGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFSDSMPC
Sbjct: 121 RGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFSDSMPC 180

Query: 181 VNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC 238
           VNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC
Sbjct: 181 VNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC 237

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BLAST of Carg11178 vs. NCBI nr
Match: KAG6581318.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 425.2 bits (1092), Expect = 3.6e-115
Identity = 233/406 (57.39%), Postives = 235/406 (57.88%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60
           MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE
Sbjct: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60

Query: 61  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------------- 120
           P NPPD++DGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA              
Sbjct: 61  PANPPDNNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVILVP 120

Query: 121 ---------------------------------------------------FNNMSLRGN 180
                                                               NNMSLRGN
Sbjct: 121 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPKSYSAHQWLVFYRVNNMSLRGN 180

Query: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF- 238
           GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF 
Sbjct: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFS 240

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BLAST of Carg11178 vs. NCBI nr
Match: XP_022935453.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 392.9 bits (1008), Expect = 2.0e-105
Identity = 233/479 (48.64%), Postives = 234/479 (48.85%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQP--PSPSPSPPLSPS 60
           MNS FCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQP  PSPSPSPPLSPS
Sbjct: 1   MNSPFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPS 60

Query: 61  PEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA------------ 120
           PEP NPPDD+DGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA            
Sbjct: 61  PEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVIL 120

Query: 121 -----------------------------------------------------FNNMSLR 180
                                                                 NNMSLR
Sbjct: 121 VPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLR 180

Query: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 238
           GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH
Sbjct: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 240

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BLAST of Carg11178 vs. NCBI nr
Match: XP_023529142.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.2 bits (962), Expect = 4.3e-100
Identity = 224/477 (46.96%), Postives = 229/477 (48.01%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60
           MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHH HFTISQPPSPSP    S SPE
Sbjct: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSP----SSSPE 60

Query: 61  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------------- 120
           P NPPDD+DGGSSFNATGFFDVRHYGAIGDGTTDDTEAFK+AWDTA              
Sbjct: 61  PANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVP 120

Query: 121 ---------------------------------------------------FNNMSLRGN 180
                                                               NNMSLRGN
Sbjct: 121 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGN 180

Query: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF- 238
           GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMS+NLTVEGLRIKDSPQFHF 
Sbjct: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFR 240

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BLAST of Carg11178 vs. NCBI nr
Match: XP_022983334.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 5.4e-95
Identity = 222/488 (45.49%), Postives = 225/488 (46.11%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPL----- 60
           MNS  CSFALCI LIIFFFPVHGRWHNHA NHKHSHHH HFTISQPPSPS S        
Sbjct: 1   MNSPLCSFALCIILIIFFFPVHGRWHNHA-NHKHSHHHHHFTISQPPSPSTSASASTSTS 60

Query: 61  -SPSPEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------- 120
            SPSPEP NPPDD+DGGSSFN+TGFFDVRHYGAIGDGTTDDTEAFKMAWDTA        
Sbjct: 61  PSPSPEPANPPDDNDGGSSFNSTGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDDKNN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 NNDNHAVILVPYGFSFMIQSTIFSGPCKHGVVFQVDGTLIAPDGPDTWPKSYSAHQWLVF 180

Query: 181 --FNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGL 238
              NNMSLRGNGVIDGRGQKWWDLPCKPHRGGKG TKAGPCDSPIAIRFFMSSNL+VEGL
Sbjct: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGSTKAGPCDSPIAIRFFMSSNLSVEGL 240

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BLAST of Carg11178 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23
Identity = 76/235 (32.34%), Postives = 98/235 (41.70%), Query Frame = 0


Query: 12  ITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPL-----------SPSPE 71
           + + I    + G + N A+   H H   H  ++ P  P P PPL            P P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH-GVTHPLPPPPPPPLETANPPDQVPSDPYPS 67

Query: 72  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------------- 131
           P   P D D G        FDV  +GA+GDG+ DDT AF+ AW  A              
Sbjct: 68  PDPAPGDSDSGC------VFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 127

Query: 132 ------------------------------------------------FNNMSLRGNGVI 174
                                                            +  +  G G +
Sbjct: 128 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 187

HSP 2 Score: 83.6 bits (205), Expect = 3.4e-15
Identity = 35/78 (44.87%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 150 AIRFFMSSNLTVEGLRIKDSPQFHF--SDSMPCVNLTLANIELFPSKGDIFLDPFCWNAY 209
           A++ F      ++G     SP  HF  SD++ C N+T++ +EL P +G++  DPFCWNAY
Sbjct: 385 AVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAY 444

Query: 210 GDLQTLTIPPLGCLLEAS 226
           G  +TLTIPP+ CLL+ S
Sbjct: 445 GKQETLTIPPIDCLLDGS 462

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BLAST of Carg11178 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-10
Identity = 47/161 (29.19%), Postives = 63/161 (39.13%), Query Frame = 0


Query: 72  SSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-----------------FNNM---- 131
           S  +A   F+V  YGA+GDG  D TEAF  AW  A                  NN+    
Sbjct: 51  SRHDAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNG 110

Query: 132 --------------------------------------SLRGNGVIDGRGQKWWDLPCKP 174
                                                 +L G GVIDG+G++WW   CK 
Sbjct: 111 PCQPHFTFKVDGIIAAYQNPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK- 170

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BLAST of Carg11178 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 46/161 (28.57%), Postives = 62/161 (38.51%), Query Frame = 0


Query: 72  SSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-----------------FNNM---- 131
           S  +A   F+V  YGA+GDG  D TEAF   W+ A                  NN+    
Sbjct: 51  SRHDAATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRG 110

Query: 132 --------------------------------------SLRGNGVIDGRGQKWWDLPCKP 174
                                                 +L G GVIDG+GQ+WW   CK 
Sbjct: 111 PCQPHLSFKVDGTIVAQPDPARWKNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV 170

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BLAST of Carg11178 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 45/170 (26.47%), Postives = 65/170 (38.24%), Query Frame = 0


Query: 82  VRHYGAIGDGTTDDTEAFKMAWDTA----------------------------------- 141
           V  +GA GDG TDDT+AF  AW  A                                   
Sbjct: 70  VSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILTVQ 129

Query: 142 -------------------------FNNMSLRG--NGVIDGRGQKWWDLPCKPHRGGKGR 189
                                     NN+S+ G   GV+DG G+ WW   CK       R
Sbjct: 130 IFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCK-------R 189

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BLAST of Carg11178 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 44/172 (25.58%), Postives = 65/172 (37.79%), Query Frame = 0


Query: 82  VRHYGAIGDGTTDDTEAFKMAWDTA----------------------------------- 141
           V ++GA GDG TDDT+AFK AW  A                                   
Sbjct: 70  VSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRNFQ 129

Query: 142 -------------------------FNNMSLRG--NGVIDGRGQKWWDLPCKPHRGGKGR 191
                                     NN+S+ G   G+I+G G+ WW   CK        
Sbjct: 130 ILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCK-------I 189

[Close]

BLAST of Carg11178 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 9.5e-106
Identity = 233/479 (48.64%), Postives = 234/479 (48.85%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQP--PSPSPSPPLSPS 60
           MNS FCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQP  PSPSPSPPLSPS
Sbjct: 1   MNSPFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPS 60

Query: 61  PEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA------------ 120
           PEP NPPDD+DGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA            
Sbjct: 61  PEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVIL 120

Query: 121 -----------------------------------------------------FNNMSLR 180
                                                                 NNMSLR
Sbjct: 121 VPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLR 180

Query: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 238
           GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH
Sbjct: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 240

[Close]

BLAST of Carg11178 vs. ExPASy TrEMBL
Match: A0A6J1J721 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481948 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.6e-95
Identity = 222/488 (45.49%), Postives = 225/488 (46.11%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPL----- 60
           MNS  CSFALCI LIIFFFPVHGRWHNHA NHKHSHHH HFTISQPPSPS S        
Sbjct: 1   MNSPLCSFALCIILIIFFFPVHGRWHNHA-NHKHSHHHHHFTISQPPSPSTSASASTSTS 60

Query: 61  -SPSPEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------- 120
            SPSPEP NPPDD+DGGSSFN+TGFFDVRHYGAIGDGTTDDTEAFKMAWDTA        
Sbjct: 61  PSPSPEPANPPDDNDGGSSFNSTGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDDKNN 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 NNDNHAVILVPYGFSFMIQSTIFSGPCKHGVVFQVDGTLIAPDGPDTWPKSYSAHQWLVF 180

Query: 181 --FNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGL 238
              NNMSLRGNGVIDGRGQKWWDLPCKPHRGGKG TKAGPCDSPIAIRFFMSSNL+VEGL
Sbjct: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGSTKAGPCDSPIAIRFFMSSNLSVEGL 240

[Close]

BLAST of Carg11178 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 7.6e-71
Identity = 187/486 (38.48%), Postives = 202/486 (41.56%), Query Frame = 0


Query: 7   SFALCITLIIFF----FPVHGRWHNHAKNHK-HSHHHPHFTISQPPSPSPSPPLSPSP-- 66
           SF  CIT IIFF    FPV+GRWHNHAK HK HSHHH HFTISQPPSP PSP  SPSP  
Sbjct: 10  SFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSP 69

Query: 67  ----EPTNPPDDDDG-GSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFN------ 126
               EP +PP+DD G  SS N+T  FDVRH+GAIGDGT DDT AFKMAWDTA        
Sbjct: 70  SPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDD 129

Query: 127 ------------------------------------------------------------ 186
                                                                       
Sbjct: 130 NNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYR 189

Query: 187 --NMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRI 238
             NMSL+GNG+IDGRGQKWWDLPCKPH   KG+T  GPCDSPIAIRFFMSS L VEG+RI
Sbjct: 190 VYNMSLQGNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSKLRVEGVRI 249

[Close]

BLAST of Carg11178 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.7e-67
Identity = 178/478 (37.24%), Postives = 194/478 (40.59%), Query Frame = 0


Query: 12  ITLIIFF----FPVHGRWHNHAKNHK-HSHHHPHFTISQPPSPSPSPPLSPSPEPTNPPD 71
           +T IIFF    FPVHGRWHNHAK HK HSHHH HFTISQPPSP      SPS EP   P 
Sbjct: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSP------SPSSEPAGSPP 60

Query: 72  DDDGG---SSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFN--------------- 131
           +D+GG   SS N+T  FDVRH+GAIGDGT DDT AFKMAWDTA                 
Sbjct: 61  EDNGGPSSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILV 120

Query: 132 ------------------------------------------------------NMSLRG 191
                                                                 NMSL+G
Sbjct: 121 PYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQG 180

Query: 192 NGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF 238
           NG+IDGRGQKWWDLPCKPH   KG+T  GPCDSPIAIRFFMSS L VEG+RIKDSPQFHF
Sbjct: 181 NGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHF 240

[Close]

BLAST of Carg11178 vs. ExPASy TrEMBL
Match: A0A5A7T924 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003270 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 5.0e-62
Identity = 178/486 (36.63%), Postives = 195/486 (40.12%), Query Frame = 0


Query: 7   SFALCITLIIFF----FPVHGRWHNHAKNHK-HSHHHPHFTISQPPSPSPSPPLSPSP-- 66
           SF  CIT IIFF    FPV+GRWHNHAK HK HSHHH HFTISQPPSP PSP  SPSP  
Sbjct: 10  SFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSP 69

Query: 67  ----EPTNPPDDDDG-GSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFN------ 126
               EP +PP+DD G  SS N+T  FDVRH+GAIGDGT DDT AFKMAWDTA        
Sbjct: 70  SPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDD 129

Query: 127 ------------------------------------------------------------ 186
                                                                       
Sbjct: 130 NNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYR 189

Query: 187 --NMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRI 238
             NMSL+GNG+IDGRGQKWWDLPCKPH   K ++ +       AIRFFMSS L VEG+RI
Sbjct: 190 VYNMSLQGNGLIDGRGQKWWDLPCKPH---KSKSVSFFWQ---AIRFFMSSKLRVEGVRI 249

[Close]

BLAST of Carg11178 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 8.1e-41
Identity = 132/477 (27.67%), Postives = 167/477 (35.01%), Query Frame = 0


Query: 12  ITLIIFFFPV-HGRWHNHAKNHKHSHHH--------PHFTISQPPSPSPSPPLSPSPEPT 71
           +TLI+    +   R H+H + HKH++H+        P  +ISQPP+P P PP SP+P   
Sbjct: 15  MTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSLP 74

Query: 72  NPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTAFNN------------- 131
             P DD    +    G ++VR YGA+GDG TDDTEAFK AWD++ NN             
Sbjct: 75  PSPSDDPADDN---NGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYG 134

Query: 132 ----------------------------------------------------MSLRGNGV 191
                                                               M+L+G GV
Sbjct: 135 YTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGV 194

Query: 192 IDGRGQKWWDLPCKPHRG-GKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF-- 238
           IDGRGQKWWDLPCKPHR   K     GPCDSPIA+RFFMSSNL VEGL+IK+SPQFHF  
Sbjct: 195 IDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRF 254

[Close]

BLAST of Carg11178 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 78/201 (38.81%), Postives = 94/201 (46.77%), Query Frame = 0


Query: 40  HFTISQPPSPSPSPPLSPSPEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAF 99
           H   SQPPSPSP+P   PS  P+   D D           +DVR YGA+G+G  DDT +F
Sbjct: 33  HHQASQPPSPSPNPN-DPSKSPSRSQDLDH-------EVVYDVRKYGAVGNGVADDTVSF 92

Query: 100 KMAWDTA----------------------------------------------------- 159
           K AWD+A                                                     
Sbjct: 93  KTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWP 152

Query: 160 -------------FNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTK-AGPCDSPIAIR 174
                         N M+L+G GVIDGRGQ WWDLPCKPH+    +TK AGPC+SP A+R
Sbjct: 153 SSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALR 212

HSP 2 Score: 77.0 bits (188), Expect = 2.2e-14
Identity = 35/71 (49.30%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 169 SPQFHF--SDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASS 228
           SP  HF  S+++PC NLTL+NIEL PSK DI + PFCWNAYG     ++P + CL    S
Sbjct: 395 SPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSNPS 454

Query: 229 RSILETQRDYC 238
            S+L      C
Sbjct: 455 TSLLSGLSGRC 465

[Close]

BLAST of Carg11178 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-26
Identity = 68/183 (37.16%), Postives = 86/183 (46.99%), Query Frame = 0


Query: 55  LSPSPEPTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------- 114
           LSPSP P    +D+D      A   FDV  +GAIGD +TDDT AFKMAWD A        
Sbjct: 27  LSPSPAPNPAYNDNDN----IAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKS 86

Query: 115 --------------------------------------------------------FNNM 174
                                                                    N +
Sbjct: 87  ALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGL 146

HSP 2 Score: 78.6 bits (192), Expect = 7.7e-15
Identity = 34/58 (58.62%), Postives = 44/58 (75.86%), Query Frame = 0


Query: 169 SPQFHF--SDSMPCVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEA 225
           SP  HF  SDS+PC NLTL  ++LFPSKG    +PFCWNAYG ++ +T+PP+ CLL+A
Sbjct: 374 SPPIHFGCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDA 430

[Close]

BLAST of Carg11178 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.1e-24
Identity = 76/235 (32.34%), Postives = 98/235 (41.70%), Query Frame = 0


Query: 12  ITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPL-----------SPSPE 71
           + + I    + G + N A+   H H   H  ++ P  P P PPL            P P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH-GVTHPLPPPPPPPLETANPPDQVPSDPYPS 67

Query: 72  PTNPPDDDDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTA-------------- 131
           P   P D D G        FDV  +GA+GDG+ DDT AF+ AW  A              
Sbjct: 68  PDPAPGDSDSGC------VFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 127

Query: 132 ------------------------------------------------FNNMSLRGNGVI 174
                                                            +  +  G G +
Sbjct: 128 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 187

HSP 2 Score: 83.6 bits (205), Expect = 2.4e-16
Identity = 35/78 (44.87%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 150 AIRFFMSSNLTVEGLRIKDSPQFHF--SDSMPCVNLTLANIELFPSKGDIFLDPFCWNAY 209
           A++ F      ++G     SP  HF  SD++ C N+T++ +EL P +G++  DPFCWNAY
Sbjct: 385 AVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAY 444

Query: 210 GDLQTLTIPPLGCLLEAS 226
           G  +TLTIPP+ CLL+ S
Sbjct: 445 GKQETLTIPPIDCLLDGS 462

[Close]

BLAST of Carg11178 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-13
Identity = 33/81 (40.74%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 159 LTVEGLRIKDSPQFHFSDSMPCVNLTLANIELFPSKGDI-FLDPFCWNAYGDLQTLTIPP 218
           + ++G   K+  +F  SDS+PC  ++L+ IEL P+ G    LDPFCW A+G+L+T T+PP
Sbjct: 355 INIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPFCWKAHGELKTKTLPP 414

Query: 219 LGCL-LEASSRSILETQRDYC 238
           + CL  E S  +   +  D C
Sbjct: 415 IQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:

BLAST of Carg11178 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018039.1	7.2e-140	100.00	Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581318.1	3.6e-115	57.39	Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022935453.1	2.0e-105	48.64	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_023529142.1	4.3e-100	46.96	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983334.1	5.4e-95	45.49	polygalacturonase At1g48100-like [Cucurbita maxima]	[more]

BLAST of Carg11178 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.5e-23	32.34	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
P43212	1.5e-10	29.19	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	2.1e-09	28.57	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q8RY29	2.3e-08	26.47	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
O23147	1.2e-07	25.58	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of Carg11178 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAK7	9.5e-106	48.64	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 P...	[more]
A0A6J1J721	2.6e-95	45.49	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481948 PE=...	[more]
A0A5D3DA93	7.6e-71	38.48	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00...	[more]
A0A0A0LEQ6	8.7e-67	37.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1	[more]
A0A5A7T924	5.0e-62	36.63	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003...	[more]

BLAST of Carg11178 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02460.1	8.1e-41	27.67	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.3e-27	38.81	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	1.5e-26	37.16	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	1.1e-24	32.34	Pectin lyase-like superfamily protein	[more]
AT5G14650.1	2.5e-13	40.74	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 106..195 e-value: 5.4E-11 score: 42.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 108..201 e-value: 1.4E-15 score: 59.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 67..107 e-value: 3.3E-9 score: 38.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..64
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 29..73
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 8..107 coord: 108..174
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 175..227 coord: 8..107
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 175..227
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 108..174
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 76..175
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 175..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11178-RA	Carg11178-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity