Carg11125 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11125
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein RALF-like 1
LocationCarg_Chr14: 4780611 .. 4780976 (-)
RNA-Seq ExpressionCarg11125
SyntenyCarg11125
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAACTCTTCTTCTCCACCTGCCTTCTTCTCTATGCTCATTTTCACCTTAGCCTTCTTTGTCTCTTCGTCCTCTTTAATGGTCGTGGCCATGAGTGGTGACCGTAGTCTTAGCTGGCTCTCGACTGAACCTCGATGCCATGGACGTTCGTTAAGTGAATGCATGATGAATGTTGAGCTTGAAATGGATTCTGAGATCAATCGTCGTATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAAGGCGAACAACATTCCATGCTCTCGACGAGGTTCGTCCTACTACAATTGCCAGCCCGGGGCTGAAGCTAACCCCTATCAGCGGGGTTGTACTGTCATTACTCGTTGCAGAAGCTAA

mRNA sequence

ATGGGCAACTCTTCTTCTCCACCTGCCTTCTTCTCTATGCTCATTTTCACCTTAGCCTTCTTTGTCTCTTCGTCCTCTTTAATGGTCGTGGCCATGAGTGGTGACCGTAGTCTTAGCTGGCTCTCGACTGAACCTCGATGCCATGGACGTTCGTTAAGTGAATGCATGATGAATGTTGAGCTTGAAATGGATTCTGAGATCAATCGTCGTATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAAGGCGAACAACATTCCATGCTCTCGACGAGGTTCGTCCTACTACAATTGCCAGCCCGGGGCTGAAGCTAACCCCTATCAGCGGGGTTGTACTGTCATTACTCGTTGCAGAAGCTAA

Coding sequence (CDS)

ATGGGCAACTCTTCTTCTCCACCTGCCTTCTTCTCTATGCTCATTTTCACCTTAGCCTTCTTTGTCTCTTCGTCCTCTTTAATGGTCGTGGCCATGAGTGGTGACCGTAGTCTTAGCTGGCTCTCGACTGAACCTCGATGCCATGGACGTTCGTTAAGTGAATGCATGATGAATGTTGAGCTTGAAATGGATTCTGAGATCAATCGTCGTATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAAGGCGAACAACATTCCATGCTCTCGACGAGGTTCGTCCTACTACAATTGCCAGCCCGGGGCTGAAGCTAACCCCTATCAGCGGGGTTGTACTGTCATTACTCGTTGCAGAAGCTAA

Protein sequence

MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCRS
Homology
BLAST of Carg11125 vs. NCBI nr
Match: KAG6581259.1 (Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017986.1 Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.1 bits (614), Expect = 4.9e-60
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE
Sbjct: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
           LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. NCBI nr
Match: XP_022934551.1 (protein RALF-like 1 [Cucurbita moschata])

HSP 1 Score: 233.8 bits (595), Expect = 7.8e-58
Identity = 117/121 (96.69%), Postives = 119/121 (98.35%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFT+AFFVSSSSLMVVAMSGDRSLSWLSTE RCHGRSLSECMM+VE
Sbjct: 1   MGNSSSPPAFFSMLIFTIAFFVSSSSLMVVAMSGDRSLSWLSTEARCHGRSLSECMMDVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. NCBI nr
Match: XP_022983404.1 (protein RALF-like 1 [Cucurbita maxima])

HSP 1 Score: 232.3 bits (591), Expect = 2.3e-57
Identity = 116/121 (95.87%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFT+AFFVSSSSLMV+AMSGDRSLSWLSTE RCHGR LSECMMNVE
Sbjct: 1   MGNSSSPPAFFSMLIFTIAFFVSSSSLMVMAMSGDRSLSWLSTEARCHGRLLSECMMNVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. NCBI nr
Match: XP_023529110.1 (protein RALF-like 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 230.3 bits (586), Expect = 8.6e-57
Identity = 115/121 (95.04%), Postives = 117/121 (96.69%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFT+AFFV SSSLMV+AMSGD SLSWLSTE RCHGRSLSECMMNVE
Sbjct: 1   MGNSSSPPAFFSMLIFTIAFFVYSSSLMVMAMSGDHSLSWLSTEARCHGRSLSECMMNVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. NCBI nr
Match: KAA0044366.1 (protein RALF-like 33 [Cucumis melo var. makuwa])

HSP 1 Score: 209.5 bits (532), Expect = 1.6e-50
Identity = 105/121 (86.78%), Postives = 112/121 (92.56%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPA F++LIFT+A FVSSSSL V AMS D+SL+WLSTE RCHGRS+SECMM +E
Sbjct: 1   MGNSSSPPA-FTILIFTIAVFVSSSSLAVTAMSSDQSLNWLSTEVRCHGRSISECMMKIE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCT ITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 120

BLAST of Carg11125 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 5.3e-25
Identity = 61/114 (53.51%), Postives = 78/114 (68.42%), Query Frame = 0

Query: 11  FSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPR---CHGRSLSECMMNVELEMDSEI 70
           F  L   + F +SS  +     +    ++W +T      CHG S++EC+   E EMDSEI
Sbjct: 8   FLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHG-SIAECIGAEEEEMDSEI 67

Query: 71  NRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCRS 122
           NRRILAT+ YISY+SLK N++PCSRRG+SYYNCQ GA+ANPY RGC+ I RCRS
Sbjct: 68  NRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

BLAST of Carg11125 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 5.0e-23
Identity = 58/123 (47.15%), Postives = 81/123 (65.85%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNV- 60
           M   S+ P    + I T+ F       +  A++   S  ++  E +C+G +++EC ++  
Sbjct: 1   MRGLSTKPVAIIIAILTVHF-------LFAAVTSQSSGDFVPIESKCNG-TIAECSLSTA 60

Query: 61  --ELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVIT 120
             E EMDSEINRRILAT+ YISY +L+ N +PCSRRG+SYYNC+ GA+ANPY RGC+ IT
Sbjct: 61  EEEFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAIT 115

BLAST of Carg11125 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 9.4e-22
Identity = 59/119 (49.58%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 8   PAFFSMLIFTLAFFVSSSSLMVVAMSGDR-SLSWL---STEPRCHGRSLSECMM-NVELE 67
           P+   + +   AFF+S      +A +GD  +  W+    +   C G S+ EC+    E E
Sbjct: 4   PSGLILCVLIGAFFIS------MAAAGDSGAYDWVMPARSGGGCKG-SIGECIAEEEEFE 63

Query: 68  MDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCRS 122
           +DSE NRRILAT  YISY +L+ N++PCSRRG+SYYNC+PGA+ANPY RGC+ ITRCRS
Sbjct: 64  LDSESNRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

BLAST of Carg11125 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-20
Identity = 61/137 (44.53%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECM---- 60
           +  +S   A F++L   L   V + S+ V + S + +  +   E  C G +++EC     
Sbjct: 4   LSRNSGAAAIFAIL---LILAVHNWSVAVSSQSTEFAGDFPPFETECRG-TIAECSVSAA 63

Query: 61  -------------MNVELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPG 120
                        M  E EMDSEINRRILAT  YISY +L+ N IPCSRRG+SYYNC+ G
Sbjct: 64  LGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRG 123

BLAST of Carg11125 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.9e-20
Identity = 46/75 (61.33%), Postives = 60/75 (80.00%), Query Frame = 0

Query: 47  CHGRSLSECMM-NVELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAE 106
           C G S++EC+    E+E DS+I+RRILA   YISY +++ N++PCSRRG+SYYNCQ GA+
Sbjct: 45  CTG-SIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 107 ANPYQRGCTVITRCR 121
           ANPY RGC+ ITRCR
Sbjct: 105 ANPYSRGCSTITRCR 118

BLAST of Carg11125 vs. ExPASy TrEMBL
Match: A0A6J1F244 (protein RALF-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111441696 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.8e-58
Identity = 117/121 (96.69%), Postives = 119/121 (98.35%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFT+AFFVSSSSLMVVAMSGDRSLSWLSTE RCHGRSLSECMM+VE
Sbjct: 1   MGNSSSPPAFFSMLIFTIAFFVSSSSLMVVAMSGDRSLSWLSTEARCHGRSLSECMMDVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. ExPASy TrEMBL
Match: A0A6J1J798 (protein RALF-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111482012 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-57
Identity = 116/121 (95.87%), Postives = 118/121 (97.52%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPAFFSMLIFT+AFFVSSSSLMV+AMSGDRSLSWLSTE RCHGR LSECMMNVE
Sbjct: 1   MGNSSSPPAFFSMLIFTIAFFVSSSSLMVMAMSGDRSLSWLSTEARCHGRLLSECMMNVE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

BLAST of Carg11125 vs. ExPASy TrEMBL
Match: A0A5A7TQI0 (Protein RALF-like 33 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00930 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.6e-51
Identity = 105/121 (86.78%), Postives = 112/121 (92.56%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVE 60
           MGNSSSPPA F++LIFT+A FVSSSSL V AMS D+SL+WLSTE RCHGRS+SECMM +E
Sbjct: 1   MGNSSSPPA-FTILIFTIAVFVSSSSLAVTAMSSDQSLNWLSTEVRCHGRSISECMMKIE 60

Query: 61  LEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCR 120
            EMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCT ITRCR
Sbjct: 61  FEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 120

BLAST of Carg11125 vs. ExPASy TrEMBL
Match: A0A0A0KWI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001640 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.5e-49
Identity = 104/123 (84.55%), Postives = 113/123 (91.87%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFV--SSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMN 60
           MG+SSSPPA  S+LIFT+AFFV  SSSSL+V  MS DRSL+WLSTE RCHGRS+SECMM+
Sbjct: 1   MGSSSSPPA-LSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMH 60

Query: 61  VELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITR 120
           +E EMDSEINRRILATSSYISYKSL+ANNIPCSRRGSSYYNCQPGAEANPYQRGCT ITR
Sbjct: 61  IEFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITR 120

Query: 121 CRS 122
           CRS
Sbjct: 121 CRS 122

BLAST of Carg11125 vs. ExPASy TrEMBL
Match: A0A5B6YRY9 (Putative Ralf-like 33 OS=Davidia involucrata OX=16924 GN=Din_003849 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.4e-28
Identity = 71/110 (64.55%), Postives = 83/110 (75.45%), Query Frame = 0

Query: 12  SMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVELEMDSEINRRI 71
           SML+   A  +SSS+  V A       SW+ T P C G S++ECM N EL+MDSEINRRI
Sbjct: 15  SMLV--AALIISSST--VDATGDHHRFSWMPTRPACQG-SIAECMANGELDMDSEINRRI 74

Query: 72  LATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCRS 122
           LATSSYISY +L+ N +PCSRRG+SYYNCQPGAEANPY RGC+ ITRCRS
Sbjct: 75  LATSSYISYGALQRNTVPCSRRGASYYNCQPGAEANPYSRGCSTITRCRS 119

BLAST of Carg11125 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 115.2 bits (287), Expect = 3.8e-26
Identity = 61/114 (53.51%), Postives = 78/114 (68.42%), Query Frame = 0

Query: 11  FSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPR---CHGRSLSECMMNVELEMDSEI 70
           F  L   + F +SS  +     +    ++W +T      CHG S++EC+   E EMDSEI
Sbjct: 8   FLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHG-SIAECIGAEEEEMDSEI 67

Query: 71  NRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRCRS 122
           NRRILAT+ YISY+SLK N++PCSRRG+SYYNCQ GA+ANPY RGC+ I RCRS
Sbjct: 68  NRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

BLAST of Carg11125 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 108.6 bits (270), Expect = 3.5e-24
Identity = 58/123 (47.15%), Postives = 81/123 (65.85%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNV- 60
           M   S+ P    + I T+ F       +  A++   S  ++  E +C+G +++EC ++  
Sbjct: 1   MRGLSTKPVAIIIAILTVHF-------LFAAVTSQSSGDFVPIESKCNG-TIAECSLSTA 60

Query: 61  --ELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAEANPYQRGCTVIT 120
             E EMDSEINRRILAT+ YISY +L+ N +PCSRRG+SYYNC+ GA+ANPY RGC+ IT
Sbjct: 61  EEEFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAIT 115

BLAST of Carg11125 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 100.1 bits (248), Expect = 1.3e-21
Identity = 61/137 (44.53%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 1   MGNSSSPPAFFSMLIFTLAFFVSSSSLMVVAMSGDRSLSWLSTEPRCHGRSLSECM---- 60
           +  +S   A F++L   L   V + S+ V + S + +  +   E  C G +++EC     
Sbjct: 4   LSRNSGAAAIFAIL---LILAVHNWSVAVSSQSTEFAGDFPPFETECRG-TIAECSVSAA 63

Query: 61  -------------MNVELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPG 120
                        M  E EMDSEINRRILAT  YISY +L+ N IPCSRRG+SYYNC+ G
Sbjct: 64  LGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRG 123

BLAST of Carg11125 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 99.0 bits (245), Expect = 2.8e-21
Identity = 46/75 (61.33%), Postives = 60/75 (80.00%), Query Frame = 0

Query: 47  CHGRSLSECMM-NVELEMDSEINRRILATSSYISYKSLKANNIPCSRRGSSYYNCQPGAE 106
           C G S++EC+    E+E DS+I+RRILA   YISY +++ N++PCSRRG+SYYNCQ GA+
Sbjct: 45  CTG-SIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 107 ANPYQRGCTVITRCR 121
           ANPY RGC+ ITRCR
Sbjct: 105 ANPYSRGCSTITRCR 118

BLAST of Carg11125 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 81.3 bits (199), Expect = 6.0e-16
Identity = 46/95 (48.42%), Postives = 61/95 (64.21%), Query Frame = 0

Query: 26  SLMVVAMSGDRSLSWLSTEPRCHGRSLSECMMNVELEMDSEINRRILAT-SSYISYKSLK 85
           +L VVA S   + +W  T+   +G+        ++  MDSE NRR LA   SYISY +L+
Sbjct: 13  TLAVVAESA--NATWTLTKSCVNGQGCIGEDGELDYLMDSETNRRQLAARRSYISYGALR 72

Query: 86  ANNIPCSRRGSSYYNCQPGAEANPYQRGCTVITRC 120
            NN+PCSRRG SYY+C+    ANPY+RGC+VIT C
Sbjct: 73  KNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHC 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6581259.14.9e-60100.00Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017986... [more]
XP_022934551.17.8e-5896.69protein RALF-like 1 [Cucurbita moschata][more]
XP_022983404.12.3e-5795.87protein RALF-like 1 [Cucurbita maxima][more]
XP_023529110.18.6e-5795.04protein RALF-like 33 [Cucurbita pepo subsp. pepo][more]
KAA0044366.11.6e-5086.78protein RALF-like 33 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SRY35.3e-2553.51Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1[more]
Q8L9P85.0e-2347.15Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1[more]
Q945T09.4e-2249.58Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1[more]
Q9LUS71.8e-2044.53Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV... [more]
Q9MA623.9e-2061.33Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2443.8e-5896.69protein RALF-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111441696 PE=3 SV=1[more]
A0A6J1J7981.1e-5795.87protein RALF-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111482012 PE=3 SV=1[more]
A0A5A7TQI07.6e-5186.78Protein RALF-like 33 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G... [more]
A0A0A0KWI22.5e-4984.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001640 PE=3 SV=1[more]
A0A5B6YRY91.4e-2864.55Putative Ralf-like 33 OS=Davidia involucrata OX=16924 GN=Din_003849 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02900.13.8e-2653.51rapid alkalinization factor 1 [more]
AT4G15800.13.5e-2447.15ralf-like 33 [more]
AT3G16570.11.3e-2144.53rapid alkalinization factor 23 [more]
AT3G05490.12.8e-2161.33ralf-like 22 [more]
AT2G33775.16.0e-1648.42ralf-like 19 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 60..120
e-value: 1.2E-27
score: 96.7
NoneNo IPR availablePANTHERPTHR33136RAPID ALKALINIZATION FACTOR-LIKEcoord: 9..121
NoneNo IPR availablePANTHERPTHR33136:SF50PROTEIN RALF-LIKE 33coord: 9..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11125-RACarg11125-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019722 calcium-mediated signaling
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane