Carg11117 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11117
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Malic enzyme
Location	Carg_Chr08: 5428146 .. 5430445 (+)
RNA-Seq Expression	Carg11117
Synteny	Carg11117

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCCCGGCCCCTTCCCGTCTCCAATATTCTTGTACAGCTTCAACTGCTTCCGCTATATTTTCACTGCAACATTTATGTTTCCACTGCGATGAACGAAGCCTCTACAATTTCAATCTCTTTAAATGAAACTCAATTAGCATTGCTTTGATTCACGGCGTCGATTCTACGAGCTTTGGATCCTATTTTTTCTAATCTGATTGGAACATTTCTTGGAGTAGAGTATTGGATTTGAGGCAGTGCTGCATTTGGTTTTTTGATTATCAGATTGGTCTTTTCTTGATTCTTTTTTTCTAGATCCAGTTTGATGTGGAACCTTGCGAGGTTAACTTTGGCGAGTTTGCGCCGATCTAGGCGGTTCTCGACTGCGATTCCTGGACCGTGTATTGTCCATAAGCGCGGTGCTGATATTCTCCATGATCCTTGGTTCAACAAGGTGCGTTGCTTCCTTTGTTTTCTTTGATTCTTCATGTTTGGTAGTTTTTTCAGTTGGTTTCATCGTTTTTTCTTTTTTTTTTTTTTGCGTTTTCTCGTTTTGGATGCTGTTTTCGTTTAGTTGTTTGTAGGCGCGCTCGTTTGAGGTTCAGTTAATGTATGAGTTGAAATTGCTTTGTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCAAAACTATACTTTTTAGTGTTTCTTACTCAAAAGTTGAAAAGAGGATCTAAAAGTGTTTATGTGATGAACATGTTTGACAATTGTTTTATTTCGTAATGGCTATGTTTTCCTATGGTACCTCCGTTTATTGCAGTATCAGAAGTTGTAGATATTTCGATGTAGGAATTTTCTTCGGTTGTTTGCCTCGACAAAGAGATTCCAAGGAGCTTTATATTTAGTTTATTGTAATTAATCAATTATTTTTTTCAGTAGAATTAATGAATTTAAGCATTTTGTTTATATTCTGGTGTAAGCGTACGGATGAATGCAAGTGCATAAAAATACATAGTGCTCTATAAATTTAAGATAGGATCGAATTTCTGTCTGGCTTCAGAAGTGAAGATGTCCCTATTCAGGGTGATAATTGGAACATCTTCATGAATTGTGTACTTACACTTGGATGTTTCTAACATTGTAAACAAATCAAGCTTTTTTTTTAGTTATTGTGCTTCTAAAATCTGTTTTTAAGTACTCTTTGGGGCATCTCTTTAAGACTAAGGCGTTAATTCTTCAGGTTTATGTTCGCTAGGAAATTGATGTTTGATCATCACTTTTTAATTCTTTATCAGTATGTATTTTGCTCCTTTTTTATGTTTCTTAAATTCTGCAAAATTGGAAGTACCCAAATCTATGTTCCTTTTTATATTTCTAAAAAGAAAACATTCTTTATCCCATGGTTGTATCTGACATAATATCGTATCATTGTCATGTGGAACTCTCTTTAAAGTTGTCCCTTTTTATTTTCAAAGAGCAGGATACTGGATTTCCTCTCACTGAAAGAGACCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTGATATCATTTGAGCAACAATATGCTCGTTTCAGTAAGTTCATGCTTCATCTTAAGTTTTCATCACTTAGTCTACCATCAGAATGACTTAGGTCAATCCCTTAGAAAAGAAACATATCAATGGATAACTAAGAAGATTATTGTAATGAAAATTTTGTAGGCTATTACGTAGGGCAGTTAGACATTGTTTGGTAGGTTATTACGTAGTTGCTAGCTGTTAAACATTGTTTGGTAGGCTATTACGTAGTTGCTAGCTGGTAAATGTCAATTGCTAGTAGTTTAATTATTTGATTTGTTGCTGTGTTATTATTTGTCTAGTTCGTTGCAAGCAACTAGTTGGTCAATTGAAAATATCTATGATAGGGCATTGCACTTGAAGTCGCAATTTTGTTTAAAGAAATATCTTTATTGATTATGCGAGACGTTGCAACTCTGTTCTATCCATCCCTATCGTGGTTACGAAAATTACCCTTAAGTTGAGTTAATTACAATTAACAAAAGTAATATACTTACGAAAGAGATTGAATAATTGTAATCACAACTTTGGTACTGAATAAAGACTTCTTTTAGTATTTGGGAAGAATTTCAATCCCTATCAGCATTAGAGATAAAATCGTTTCTTGAATGTCTCTTGCATTTTTTGTTCAATTTCCTAGTGGAATCATATCGATCACTGGAGAGGAATACTCATGGTCAACCAGATGGTATTGTTTCATTGGCGAAATGGAGGATCTTAAATAGATTGCATGATAGAAATGAAACACTGTACTATCGAGTAAGTTGCAAAAATGTTGTTTAG

mRNA sequence

TCCCCCGGCCCCTTCCCGTCTCCAATATTCTTGTACAGCTTCAACTGCTTCCGCTATATTTTCACTGCAACATTTATGTTTCCACTGCGATGAACGAAGCCTCTACAATTTCAATCTCTTTAAATGAAACTCAATTAGCATTGCTTTGATTCACGGCGTCGATTCTACGAGCTTTGGATCCTATTTTTTCTAATCTGATTGGAACATTTCTTGGAGTAGAGTATTGGATTTGAGGCAGTGCTGCATTTGGTTTTTTGATTATCAGATTGGTCTTTTCTTGATTCTTTTTTTCTAGATCCAGTTTGATGTGGAACCTTGCGAGGTTAACTTTGGCGAGTTTGCGCCGATCTAGGCGGTTCTCGACTGCGATTCCTGGACCGTGTATTGTCCATAAGCGCGGTGCTGATATTCTCCATGATCCTTGGTTCAACAAGGATACTGGATTTCCTCTCACTGAAAGAGACCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTGATATCATTTGAGCAACAATATGCTCGTTTCATGGAATCATATCGATCACTGGAGAGGAATACTCATGGTCAACCAGATGGTATTGTTTCATTGGCGAAATGGAGGATCTTAAATAGATTGCATGATAGAAATGAAACACTGTACTATCGAGTAAGTTGCAAAAATGTTGTTTAG

Coding sequence (CDS)

ATGTGGAACCTTGCGAGGTTAACTTTGGCGAGTTTGCGCCGATCTAGGCGGTTCTCGACTGCGATTCCTGGACCGTGTATTGTCCATAAGCGCGGTGCTGATATTCTCCATGATCCTTGGTTCAACAAGGATACTGGATTTCCTCTCACTGAAAGAGACCGTCTAGGAATCCGTGGCCTTCTTCCTCCACGAGTGATATCATTTGAGCAACAATATGCTCGTTTCATGGAATCATATCGATCACTGGAGAGGAATACTCATGGTCAACCAGATGGTATTGTTTCATTGGCGAAATGGAGGATCTTAAATAGATTGCATGATAGAAATGAAACACTGTACTATCGAGTAAGTTGCAAAAATGTTGTTTAG

Protein sequence

MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKNVV

Homology

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BLAST of Carg11117 vs. NCBI nr
Match: KAG7026019.1 (hypothetical protein SDJN02_12517 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 261.2 bits (666), Expect = 4.6e-66
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120

Query: 121 VV 123
           VV
Sbjct: 121 VV 122

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BLAST of Carg11117 vs. NCBI nr
Match: XP_022964521.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Cucurbita moschata])

HSP 1 Score: 250.4 bits (638), Expect = 8.1e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

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BLAST of Carg11117 vs. NCBI nr
Match: KAG6593683.1 (hypothetical protein SDJN03_13159, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.4 bits (638), Expect = 8.1e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. NCBI nr
Match: XP_023514814.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.4 bits (638), Expect = 8.1e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. NCBI nr
Match: XP_022999821.1 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Cucurbita maxima])

HSP 1 Score: 250.4 bits (638), Expect = 8.1e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

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BLAST of Carg11117 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 2.9e-55
Identity = 97/121 (80.17%), Postives = 107/121 (88.43%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MW +AR   ++ RR+RR STAI  PCIVHKRGADILHDPWFNKDTGFP+TERDRLG+RGL
Sbjct: 1   MWRVARSAASTFRRTRRLSTAISAPCIVHKRGADILHDPWFNKDTGFPMTERDRLGLRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQY RFMES+RSLE+NT GQPD +VSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYDRFMESFRSLEKNTEGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

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BLAST of Carg11117 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.3e-47
Identity = 93/127 (73.23%), Postives = 106/127 (83.46%), Query Frame = 0


Query: 1   MW-NLARLTLAS----LRRSRR-FSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 60
           MW N+A L+ A+       SRR FSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 61  LGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYY 120
           LGIRGLLPPRV++  QQ  RF+ES+RSLE NT G+P+ +V+LAKWR+LNRLHDRNETLYY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 121 RVSCKNV 122
           RV   N+
Sbjct: 122 RVLIDNI 128

[Close]

BLAST of Carg11117 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-32
Identity = 69/108 (63.89%), Postives = 75/108 (69.44%), Query Frame = 0


Query: 15  SRRFSTAI-PGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYA 74
           SR F+T     P IVHKR  DILHDPWFNK T F  TERDRL IRGLLPP V+SFEQQ A
Sbjct: 31  SRNFTTTEGHRPTIVHKRSLDILHDPWFNKGTAFSFTERDRLHIRGLLPPNVMSFEQQIA 90

Query: 75  RFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKNV 122
           RFM   + LE      P     LAKWRILNRLHDRNETLYY+V  +N+
Sbjct: 91  RFMADLKRLEVQARDGPSDPYVLAKWRILNRLHDRNETLYYKVLMENI 138

[Close]

BLAST of Carg11117 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.2e-30
Identity = 69/131 (52.67%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 3   NLARLTLASLRR--SRR--FSTAIPG--------PCIVHKRGADILHDPWFNKDTGFPLT 62
           N  RL+ +SL R   RR  +S+A+          P IVHK+G DILHDPWFNK T F +T
Sbjct: 5   NKLRLSSSSLSRILHRRILYSSAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTMT 64

Query: 63  ERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNE 122
           ER+RL +RGLLPP V+  EQQ  RFM   + LE      P    +LAKWRILNRLHDRNE
Sbjct: 65  ERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRNE 124

[Close]

BLAST of Carg11117 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 8.8e-28
Identity = 60/114 (52.63%), Postives = 76/114 (66.67%), Query Frame = 0


Query: 9   LASLRRSRRFSTAIPGP-CIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVIS 68
           +A++   R F+T+      IV+KR  DIL DPWFNK T F +TERDRL +RGLLPP V++
Sbjct: 22  IANVSNHRSFATSEGHRLAIVNKRSLDILQDPWFNKGTAFSMTERDRLDLRGLLPPNVMT 81

Query: 69  FEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKNV 122
            EQQ  RF    R LE  T   P     LAKWRILNRLHDRNET++++V  +N+
Sbjct: 82  TEQQIERFTADLRVLELTTKDGPSDTYDLAKWRILNRLHDRNETMFFKVLIENI 135

[Close]

BLAST of Carg11117 vs. ExPASy TrEMBL
Match: A0A6J1KE67 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111494177 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.9e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. ExPASy TrEMBL
Match: A0A6J1HJ42 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111464523 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.9e-63
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. ExPASy TrEMBL
Match: A0A6J1DPR7 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111023088 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.8e-61
Identity = 113/121 (93.39%), Postives = 115/121 (95.04%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNLARLT ASL RSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLARLTAASLSRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPD +VSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. ExPASy TrEMBL
Match: A0A1S3CBH2 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103499065 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.5e-59
Identity = 110/121 (90.91%), Postives = 112/121 (92.56%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWNL + T AS RRSR  STAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL
Sbjct: 1   MWNLVKFTAASCRRSRWLSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. ExPASy TrEMBL
Match: A0A251LW48 (Malic enzyme OS=Manihot esculenta OX=3983 GN=MANES_01G260100 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 6.5e-58
Identity = 106/121 (87.60%), Postives = 113/121 (93.39%), Query Frame = 0


Query: 1   MWNLARLTLASLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGIRGL 60
           MWN+AR   +SLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLG+RGL
Sbjct: 1   MWNVARFATSSLRRSRRFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDRLGLRGL 60

Query: 61  LPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYYRVSCKN 120
           LPPRVISFEQQYARFMESYRSLE+NT GQP+ +VSLAKWRILNRLHDRNETLYYRV   N
Sbjct: 61  LPPRVISFEQQYARFMESYRSLEKNTQGQPNSVVSLAKWRILNRLHDRNETLYYRVLIDN 120

Query: 121 V 122
           +
Sbjct: 121 I 121

[Close]

BLAST of Carg11117 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 190.3 bits (482), Expect = 9.3e-49
Identity = 93/127 (73.23%), Postives = 106/127 (83.46%), Query Frame = 0


Query: 1   MW-NLARLTLAS----LRRSRR-FSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 60
           MW N+A L+ A+       SRR FSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 61  LGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNETLYY 120
           LGIRGLLPPRV++  QQ  RF+ES+RSLE NT G+P+ +V+LAKWR+LNRLHDRNETLYY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 121 RVSCKNV 122
           RV   N+
Sbjct: 122 RVLIDNI 128

[Close]

BLAST of Carg11117 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 132.1 bits (331), Expect = 3.0e-31
Identity = 69/131 (52.67%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 3   NLARLTLASLRR--SRR--FSTAIPG--------PCIVHKRGADILHDPWFNKDTGFPLT 62
           N  RL+ +SL R   RR  +S+A+          P IVHK+G DILHDPWFNK T F +T
Sbjct: 5   NKLRLSSSSLSRILHRRILYSSAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTMT 64

Query: 63  ERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPDGIVSLAKWRILNRLHDRNE 122
           ER+RL +RGLLPP V+  EQQ  RFM   + LE      P    +LAKWRILNRLHDRNE
Sbjct: 65  ERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRNE 124

[Close]

BLAST of Carg11117 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 56.2 bits (134), Expect = 2.1e-08
Identity = 31/90 (34.44%), Postives = 44/90 (48.89%), Query Frame = 0


Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  +L DP +NK   F   ERD   +RGLLPP V+  + Q  R + + R  +        
Sbjct: 42  GYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQ-------- 101

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVSCKNV 122
               L K+  L  L +RNE L+Y++   NV
Sbjct: 102 --FPLQKYMALTELQERNERLFYKLLIDNV 121

[Close]

BLAST of Carg11117 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 53.9 bits (128), Expect = 1.0e-07
Identity = 31/90 (34.44%), Postives = 44/90 (48.89%), Query Frame = 0


Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  +L DP  NK   F   ERD   +RGLLPP VIS + Q  + M + R  +        
Sbjct: 107 GYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQ-------- 166

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVSCKNV 122
             V L K+  +  L + NE L+Y++   +V
Sbjct: 167 --VPLQKYMAMMDLQETNERLFYKLLIDHV 186

[Close]

BLAST of Carg11117 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 53.1 bits (126), Expect = 1.8e-07
Identity = 31/90 (34.44%), Postives = 44/90 (48.89%), Query Frame = 0


Query: 32  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVISFEQQYARFMESYRSLERNTHGQPD 91
           G  ++ DP +NK   F   ERD   I GLLPP V+S + Q  + M + R           
Sbjct: 49  GYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQY--------- 108

Query: 92  GIVSLAKWRILNRLHDRNETLYYRVSCKNV 122
             V L ++  L  L +RNE L+Y++   NV
Sbjct: 109 -TVPLQRYMALMDLQERNERLFYKLLIDNV 128

The following BLAST results are available for this feature:

BLAST of Carg11117 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026019.1	4.6e-66	100.00	hypothetical protein SDJN02_12517 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022964521.1	8.1e-63	96.69	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Cucurbita moschata]	[more]
KAG6593683.1	8.1e-63	96.69	hypothetical protein SDJN03_13159, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023514814.1	8.1e-63	96.69	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial isoform X1 [Cucurbita p...	[more]
XP_022999821.1	8.1e-63	96.69	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Cucurbita maxima]	[more]

BLAST of Carg11117 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P37225	2.9e-55	80.17	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
Q8L7K9	1.3e-47	73.23	NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37221	1.2e-32	63.89	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
Q9SIU0	4.2e-30	52.67	NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37224	8.8e-28	52.63	NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond...	[more]

BLAST of Carg11117 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KE67	3.9e-63	96.69	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111494177 PE=3 SV=1	[more]
A0A6J1HJ42	3.9e-63	96.69	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111464523 PE=3 SV=1	[more]
A0A6J1DPR7	4.8e-61	93.39	Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111023088 PE=3 SV=1	[more]
A0A1S3CBH2	4.5e-59	90.91	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103499065 PE=3 SV=1	[more]
A0A251LW48	6.5e-58	87.60	Malic enzyme OS=Manihot esculenta OX=3983 GN=MANES_01G260100 PE=3 SV=1	[more]

BLAST of Carg11117 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G00570.1	9.3e-49	73.23	NAD-dependent malic enzyme 2	[more]
AT2G13560.1	3.0e-31	52.67	NAD-dependent malic enzyme 1	[more]
AT2G19900.1	2.1e-08	34.44	NADP-malic enzyme 1	[more]
AT1G79750.1	1.0e-07	34.44	NADP-malic enzyme 4	[more]
AT5G25880.1	1.8e-07	34.44	NADP-malic enzyme 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1370.30		coord: 30..122 e-value: 4.1E-30 score: 106.0
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 1..121
None	No IPR available	PANTHER	PTHR23406:SF72	MALIC ENZYME	coord: 1..121
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 17..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11117-RA	Carg11117-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding