Carg11066 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11066
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionent-kaurenoic acid oxidase 2-like
LocationCarg_Chr08: 5730100 .. 5734011 (+)
RNA-Seq ExpressionCarg11066
SyntenyCarg11066
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCCCTCTCCTAAAATGTACACGGCGGTGCTCGCGGCGGCGCTGGTAGCTGTAGCGGCCACAGTCTGGGTGTTGAAGAATGCGAACCGGTGGATCCACGAAACCTTCCGGTTGGGTTCAAAACGGTTCAACCTCCCACCCGGGGATCTGGGCTGGCCGTTCATCGGCAACATGTGGTCCTTTCTCAGAGCTTTCAAGTCCTCTCGTCCTGATTCCTTCATGGAGTCCTTCGTCGCCAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCAATTTCTCTCTCTTTCTCCATTGATCTGCAACTATGGAGCTTTTAAGCTAACGACCTCAAAAGTGACGTGACAATAAAAATTAAAAAAAATAAATAAATTATTTAATAGATTCTTAAAATAATTTTATTCTTCATATTTATAATATTTTATTTATAAATATTTTAATCTCGTTAGAATCTACGTCATTTATTAATTAATAAATTCGAAACTTAATCCAACAATTATTTAAATTTTTGAACTCATTAAACTCAAAATTAAATTTTATTATATTTAGATATTTATTAAAGAAAAATTAAAAATTAAAATTTTATTTTTTATTTTTTATTTTTTAAAAGTGAAAGATTATTTTAAATTTGTTTATAGGTTCACTCAGTTTTAATTTTGTTTTTTTTTTAAAATATTAGAGATAATATATTTCTAAATTTTTAATTTTGTTTAATAAATTTTTAAATTCAAAGTCTGTACCTCCATATTTATTTTAAAATTTAAAAATATTTAATTTAACCCTAATAAATCAATTCTAATTGTTGGATGATGAAAGTTACACGTCAGTTAATTTAGGGAATGATCATGGGTTTATAAGTGATGAATACTAACTTCATTGGTGTGAGGCCTTCTAGAGAAGCGCAAAGCAAAGCCATGAGAGCTTATGTTCAAAGTGGATAATGTCATACCATTGTGGAGAGTCGTGTTCGTCTAACACTAATAAATTATTAATCTAAAAAATATTAAGATTAGAGGGGGAATATTGATTATTTTGGAATATTTAAAAATTAGAACGGTCGGAAACAAAATTTTAAGCGATGGAGGTTACGTCACTTGCTTTGAAACGGTTGTTTCGTCTTTGCAACCGTTCCGTTTTGCTCAATCCTCTGAAAATGTTTACGTTATTTTACACAAAATTAATTTAAATTAATTATATGTTTTTTATAATTACAAGTTTAGAGATTAAATTATTATTTTTTTTGGGGTGATTTTATTCAATTAAGTTTAATAATAAGAAATTAAATTGAATATTTTAAATTTTCGGGAAAATCTAAGCTGGAAGACAAAGCTTAGTTTTGTCATATCAGCTGTCGGTTTCGGTTCACCAATGGCTGCCGTTTCGGCATTGGTCTGCGGATCAGTCCAACTTTCCCATCTTGGCCCGCTCCATCGTGGGCCCAATCAAATTTTACTTGGCCCATCATTGCCCCAGAAAGTCATTCTTTTATTAAAATTATAAAAAATAAAATGGCCTTTATTTATTATTATTATTATTATTATTTAGTGTTTGTCGTTTACTAATAATAAGTGAAACATCTTTTTTCATGTTTTTTACAAATGTTTGTTGAGATAAGAGAACAATATTAATAAATTAATATATATATATATATATATATATGTGTGTGTGTATCTTTTAAACTACCTTTTTGTTTTTACCATTTTAAAATGGTCGTGTTGTCTATTTGCATTTGGGAATGTCTGAAAAGTGGCATTTTTCTTAATTTTTGTGCTTTTAAGCTTTGGATGCTTCAATTACAATAAATATTACGTGTTTGTATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATTTATTTATTGAAGCATTCAAATAAAAGCATGTTTGGTATCGTTTTTGTTTTCGAATAGGTAAAGAAGTTAGCGGTTTGTATTTATTTGTTCGGGGAGTTTAAAAATTATTTTTTTAGGTATGGACGTACAGATATGTATAAATGTTTCATGTTTGGAAGTCCAAGTGTGATTGTGACATCAGCTGAAGCATGCAAAAGAGTTTTGAATGACGATGATGCGTTTGGTTCAGGATGGCCTCGTTCCACCCTGAAACTCATTGGAGAGAAGTCGTTCATTGGGATTTCTTCCAAAGAACACAAGCGTCTTCGTCGTATAACTGCCGCGCCGGTCAATGGCTACGAGGCATTGGCTACGTACCTCACGTATATAGAAAATATGACGGTTGCTTCATTGCAAAAATGGCCTTCCATGGGACAAATAGAGTTCTTAACTGAGCTACGTAAGCTTACTTTCAAGATCATTATGTACATATTTCTTAGCTCGGAGAGCGAGGTGGTGATGGAGGCTTTGGAGAGAGAGTACACAACCTTGAATTATGGTGTGAGGGCTATGGCTATCAACATTCCCGGTTTTGCTTACCATAAAGCACTCAAGGTGACTCTTTTCTATAGTATGGTTATCAAACTATATCTTAGTTAACTGTTCGAAATTGAACTTTAGCAACTCATGATTGATCGAACATATCGTTACAGGCACGAAAGAACCTCGTTATGACATTTCAATCAATTGTGAGTCAGCGAAGGAAGGAAAGGGATAATGACGTGGCAACGACGAGAAGAGATATGATGGACGCTTTGCTAGATGTAGAAGATGAGAATGGTAGAATGTTAAGTGATGAAGAAATCATTGATGTCTTGATAATGTACTTGAATGCAGGCCATGAATCTTCGGGCCATACTATGATGTGGGCAACAATTTTCCTACAACAACATCCCAATTTTCTTGGAAAGGCAAAGGTATATATACAAATTAGGGTTTGCTACAAATCACATCCTAAGCATTTGACAATATGATCCAACTCTCGAATAGGCCGAGCAAGAAGAGATACTTAAAAAACGACCTCCGGGGCAGAAAGGTTTAACTCTTAAGGAAGTTCGAGAAATGGACTATCTTTCAAAGGTACATTACCCCTTTTGATATAAGAAGAAATTTATCATAATCCATCATCTGAAATTTAACTTTTGCAGGTAATCGATGAAACACTACGAGTCGTAACGTTCTCCCTCACGGTATTTCGAGAGGCAAAGCATGACGTGAACATTTGTGGTTCGTTTAGGGTTTTCATGTTCTTCTTTTGTCATAGTATGCATTAGTAGCTTAAACCCTGTAACTTTCTCGTTTGAAGGTTATACCATTCCAAAAGGGTGGAAAGTGCTAGTTTGGTTTCGAAGTGTTCACTATGATCCTGAAACTTATCCAAACCCTAAACAGTTTGATCCTTCACGATGGGATGTAAGAACGATTCAACTCGACATTATTTATTATGATTACTTGAAAATTATATTATACGTTATGATCTCACATATATACACAATTTGAGTTCAGGATTTCACACCAAAAGCTGGAAGCTTTCTTCCATTTGGAGGAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAGCTAGAAATCTCCGTCTTTCTCCATTATTTTCTCCTCAATTATCGGTGAGCTCTCTTCATAACTACACGTGTATGACTATAAGTTGAGCTATGTTTAGATTGGCATAAAACGAGCTAACGAGATGAATTGTTTTTGTGGACAGGTTGGAAAGAGATAATCCAAATAGTCCCGTGAGGTATCTACCCCACACGAGGCCTAAAGATCAGTGTTTGGCAAGGATCAAGAGAGTTGGTGCTCCATTTGATTGAAGATTTGGATGAAATGAATGGTCTTGAAATAGTTGAGATTGTTTTTACCTCGATTGTTGTTTGGTAATGTGATTACTCGACTTTAATTGTCGTTTTAAGAGTTTACTTTGCTGAAGGACTAAAATTGGTAGCAATGTGGCCTTCTATCTTCA

mRNA sequence

CTTCCCCTCTCCTAAAATGTACACGGCGGTGCTCGCGGCGGCGCTGGTAGCTGTAGCGGCCACAGTCTGGGTGTTGAAGAATGCGAACCGGTGGATCCACGAAACCTTCCGGTTGGGTTCAAAACGGTTCAACCTCCCACCCGGGGATCTGGGCTGGCCGTTCATCGGCAACATGTGGTCCTTTCTCAGAGCTTTCAAGTCCTCTCGTCCTGATTCCTTCATGGAGTCCTTCGTCGCCAGGTATGGACGTACAGATATGTATAAATGTTTCATGTTTGGAAGTCCAAGTGTGATTGTGACATCAGCTGAAGCATGCAAAAGAGTTTTGAATGACGATGATGCGTTTGGTTCAGGATGGCCTCGTTCCACCCTGAAACTCATTGGAGAGAAGTCGTTCATTGGGATTTCTTCCAAAGAACACAAGCGTCTTCGTCGTATAACTGCCGCGCCGGTCAATGGCTACGAGGCATTGGCTACGTACCTCACGTATATAGAAAATATGACGGTTGCTTCATTGCAAAAATGGCCTTCCATGGGACAAATAGAGTTCTTAACTGAGCTACGTAAGCTTACTTTCAAGATCATTATGTACATATTTCTTAGCTCGGAGAGCGAGGTGGTGATGGAGGCTTTGGAGAGAGAGTACACAACCTTGAATTATGGTGTGAGGGCTATGGCTATCAACATTCCCGGTTTTGCTTACCATAAAGCACTCAAGGCACGAAAGAACCTCGTTATGACATTTCAATCAATTGTGAGTCAGCGAAGGAAGGAAAGGGATAATGACGTGGCAACGACGAGAAGAGATATGATGGACGCTTTGCTAGATGTAGAAGATGAGAATGGTAGAATGTTAAGTGATGAAGAAATCATTGATGTCTTGATAATGTACTTGAATGCAGGCCATGAATCTTCGGGCCATACTATGATGTGGGCAACAATTTTCCTACAACAACATCCCAATTTTCTTGGAAAGGCAAAGGCCGAGCAAGAAGAGATACTTAAAAAACGACCTCCGGGGCAGAAAGGTTTAACTCTTAAGGAAGTTCGAGAAATGGACTATCTTTCAAAGGTAATCGATGAAACACTACGAGTCGTAACGTTCTCCCTCACGGTATTTCGAGAGGCAAAGCATGACGTGAACATTTGTGGTTATACCATTCCAAAAGGGTGGAAAGTGCTAGTTTGGTTTCGAAGTGTTCACTATGATCCTGAAACTTATCCAAACCCTAAACAGTTTGATCCTTCACGATGGGATGATTTCACACCAAAAGCTGGAAGCTTTCTTCCATTTGGAGGAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAGCTAGAAATCTCCGTCTTTCTCCATTATTTTCTCCTCAATTATCGGTTGGAAAGAGATAATCCAAATAGTCCCGTGAGGTATCTACCCCACACGAGGCCTAAAGATCAGTGTTTGGCAAGGATCAAGAGAGTTGGTGCTCCATTTGATTGAAGATTTGGATGAAATGAATGGTCTTGAAATAGTTGAGATTGTTTTTACCTCGATTGTTGTTTGGTAATGTGATTACTCGACTTTAATTGTCGTTTTAAGAGTTTACTTTGCTGAAGGACTAAAATTGGTAGCAATGTGGCCTTCTATCTTCA

Coding sequence (CDS)

ATGTACACGGCGGTGCTCGCGGCGGCGCTGGTAGCTGTAGCGGCCACAGTCTGGGTGTTGAAGAATGCGAACCGGTGGATCCACGAAACCTTCCGGTTGGGTTCAAAACGGTTCAACCTCCCACCCGGGGATCTGGGCTGGCCGTTCATCGGCAACATGTGGTCCTTTCTCAGAGCTTTCAAGTCCTCTCGTCCTGATTCCTTCATGGAGTCCTTCGTCGCCAGGTATGGACGTACAGATATGTATAAATGTTTCATGTTTGGAAGTCCAAGTGTGATTGTGACATCAGCTGAAGCATGCAAAAGAGTTTTGAATGACGATGATGCGTTTGGTTCAGGATGGCCTCGTTCCACCCTGAAACTCATTGGAGAGAAGTCGTTCATTGGGATTTCTTCCAAAGAACACAAGCGTCTTCGTCGTATAACTGCCGCGCCGGTCAATGGCTACGAGGCATTGGCTACGTACCTCACGTATATAGAAAATATGACGGTTGCTTCATTGCAAAAATGGCCTTCCATGGGACAAATAGAGTTCTTAACTGAGCTACGTAAGCTTACTTTCAAGATCATTATGTACATATTTCTTAGCTCGGAGAGCGAGGTGGTGATGGAGGCTTTGGAGAGAGAGTACACAACCTTGAATTATGGTGTGAGGGCTATGGCTATCAACATTCCCGGTTTTGCTTACCATAAAGCACTCAAGGCACGAAAGAACCTCGTTATGACATTTCAATCAATTGTGAGTCAGCGAAGGAAGGAAAGGGATAATGACGTGGCAACGACGAGAAGAGATATGATGGACGCTTTGCTAGATGTAGAAGATGAGAATGGTAGAATGTTAAGTGATGAAGAAATCATTGATGTCTTGATAATGTACTTGAATGCAGGCCATGAATCTTCGGGCCATACTATGATGTGGGCAACAATTTTCCTACAACAACATCCCAATTTTCTTGGAAAGGCAAAGGCCGAGCAAGAAGAGATACTTAAAAAACGACCTCCGGGGCAGAAAGGTTTAACTCTTAAGGAAGTTCGAGAAATGGACTATCTTTCAAAGGTAATCGATGAAACACTACGAGTCGTAACGTTCTCCCTCACGGTATTTCGAGAGGCAAAGCATGACGTGAACATTTGTGGTTATACCATTCCAAAAGGGTGGAAAGTGCTAGTTTGGTTTCGAAGTGTTCACTATGATCCTGAAACTTATCCAAACCCTAAACAGTTTGATCCTTCACGATGGGATGATTTCACACCAAAAGCTGGAAGCTTTCTTCCATTTGGAGGAGGAAGTAGATTGTGTCCCGGAAACGATCTTGCAAAGCTAGAAATCTCCGTCTTTCTCCATTATTTTCTCCTCAATTATCGGTTGGAAAGAGATAATCCAAATAGTCCCGTGAGGTATCTACCCCACACGAGGCCTAAAGATCAGTGTTTGGCAAGGATCAAGAGAGTTGGTGCTCCATTTGATTGA

Protein sequence

MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKRVGAPFD
Homology
BLAST of Carg11066 vs. NCBI nr
Match: KAG6593731.1 (Ent-kaurenoic acid oxidase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026070.1 Ent-kaurenoic acid oxidase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 994.6 bits (2570), Expect = 3.1e-286
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
           MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS
Sbjct: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV
Sbjct: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVGAPFD
Sbjct: 481 IKRVGAPFD 489

BLAST of Carg11066 vs. NCBI nr
Match: XP_022964631.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita moschata])

HSP 1 Score: 989.2 bits (2556), Expect = 1.3e-284
Identity = 486/489 (99.39%), Postives = 487/489 (99.59%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTS EACKRVLNDDDAFGSGWPRSTLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSTEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
           MTFQSIVSQRRK+RDNDVATTRRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESS
Sbjct: 241 MTFQSIVSQRRKKRDNDVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV
Sbjct: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVGAPFD
Sbjct: 481 IKRVGAPFD 489

BLAST of Carg11066 vs. NCBI nr
Match: XP_023513658.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 984.6 bits (2544), Expect = 3.2e-283
Identity = 483/489 (98.77%), Postives = 486/489 (99.39%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAAT+WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATIWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
            TFQSIVSQRRKERDN+VATTRRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESS
Sbjct: 241 TTFQSIVSQRRKERDNNVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV
Sbjct: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNY+LERDNPNSPVRYLPHTRPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHTRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVG PFD
Sbjct: 481 IKRVGGPFD 489

BLAST of Carg11066 vs. NCBI nr
Match: XP_023000440.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita maxima])

HSP 1 Score: 970.3 bits (2507), Expect = 6.2e-279
Identity = 476/489 (97.34%), Postives = 483/489 (98.77%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWP STLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPHSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGIS+KEHKRLRRITAAPVNGYEALATYLTYIEN+TV+SLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISAKEHKRLRRITAAPVNGYEALATYLTYIENVTVSSLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
            TFQSIVSQRRKERDN+VATTRRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESS
Sbjct: 241 TTFQSIVSQRRKERDNNVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFL KAKAEQEEILKKR PGQKGLTLKEVREMDYLSKVIDETLR+
Sbjct: 301 GHTMMWATIFLQQHPNFLEKAKAEQEEILKKRSPGQKGLTLKEVREMDYLSKVIDETLRI 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSL VFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLMVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNY+LERDNPNSPVRYLPH+RPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHSRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVGAPFD
Sbjct: 481 IKRVGAPFD 489

BLAST of Carg11066 vs. NCBI nr
Match: XP_038907052.1 (ent-kaurenoic acid oxidase 2-like [Benincasa hispida])

HSP 1 Score: 831.6 bits (2147), Expect = 3.4e-237
Identity = 411/486 (84.57%), Postives = 439/486 (90.33%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           M+ AVL A LV     VWVLKNAN W+HET RLGSKRF LPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MFMAVLVAVLV---VAVWVLKNANHWVHETARLGSKRFALPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KS  PDSF++SF++RYG T MYK FMFGSPSVIVTSAEACKRVLNDD+AFGSGWP ST+K
Sbjct: 61  KSPYPDSFIDSFLSRYGNTGMYKAFMFGSPSVIVTSAEACKRVLNDDEAFGSGWPTSTMK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGE SFIGIS +EHKRLRRITAAPVNGY+ALATYLTYIE + V+SL+KW SMGQIEFLT
Sbjct: 121 LIGENSFIGISYQEHKRLRRITAAPVNGYDALATYLTYIEKIVVSSLEKWASMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESE +MEALEREYT LN+GVRAMAINIPGFAY+KALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEAIMEALEREYTQLNHGVRAMAINIPGFAYNKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERD--NDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHE 300
            TF SIVS RRKER+  N++   RRDMMDALLD EDE+GR LSDEEIIDVLIMYLNAGHE
Sbjct: 241 ATFGSIVSGRRKERERKNNLIAKRRDMMDALLDAEDEDGRRLSDEEIIDVLIMYLNAGHE 300

Query: 301 SSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETL 360
           SSGHTMMWATIFLQQHP FL KAK EQEEI+KKRP GQK LTLKEVREM YLSKVIDETL
Sbjct: 301 SSGHTMMWATIFLQQHPQFLQKAKVEQEEIVKKRPLGQKNLTLKEVREMPYLSKVIDETL 360

Query: 361 RVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 420
           RVVTFSLTVFREAK DV ICGYTIPKGWKVLVWFRSVHYDPETYPNPK+F+PSRWD+F P
Sbjct: 361 RVVTFSLTVFREAKQDVKICGYTIPKGWKVLVWFRSVHYDPETYPNPKEFNPSRWDNFIP 420

Query: 421 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCL 480
           KAGSFLPFG GSRLCPGNDLAKLEIS+FLHYFLLNY+LER NP SPVRYLPH+RPKD CL
Sbjct: 421 KAGSFLPFGAGSRLCPGNDLAKLEISIFLHYFLLNYQLERVNPRSPVRYLPHSRPKDNCL 480

Query: 481 ARIKRV 485
           ARIKR+
Sbjct: 481 ARIKRL 483

BLAST of Carg11066 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 690.6 bits (1781), Expect = 1.2e-197
Identity = 327/466 (70.17%), Postives = 387/466 (83.05%), Query Frame = 0

Query: 18  WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYG 77
           WVLK  N WI+ + +LG K+  LPPGDLGWP IGNMWSFLRAFK+S P+SF++S++ RYG
Sbjct: 23  WVLKRVNVWIYVS-KLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYG 82

Query: 78  RTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKR 137
           RT +YK  MFG P V+VT+ E C+RVL DDDAF  GWP+ST+KLIG KSF+GIS +EHKR
Sbjct: 83  RTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKR 142

Query: 138 LRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSS 197
           LRR+T+APVNG EAL+ Y+ +IE      L+KW  MG+IEFL+ LRKLTFK+IMYIFLSS
Sbjct: 143 LRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSS 202

Query: 198 ESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDND 257
           ESE VM++LEREYT LNYGVRAM IN+PGFAYH+ALKARK LV  FQSIV+ RR +R  +
Sbjct: 203 ESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 262

Query: 258 VATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNF 317
           +++ R+DM+D L+DV+DENGR+L DEEIID+L+MYLNAGHESSGH  MWATI +Q+HP  
Sbjct: 263 ISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMI 322

Query: 318 LGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNI 377
           L KAK EQE I+KKR PGQK LTLKE REM YLS+VIDETLRV+TFSLT FREAK DV +
Sbjct: 323 LQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQM 382

Query: 378 CGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGND 437
            GY IPKGWKVL WFR+VH DPE YP+PK+FDPSRW+ +TPKAG+FLPFG GS LCPGND
Sbjct: 383 DGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGND 442

Query: 438 LAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKR 484
           LAKLEIS+FLH+FLL YR+ER NP  PV +LPH RPKD CLARI R
Sbjct: 443 LAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of Carg11066 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.4e-195
Identity = 319/465 (68.60%), Postives = 387/465 (83.23%), Query Frame = 0

Query: 18  WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYG 77
           W+++  N W++E+  LG  R  LPPGDLGWPFIGNM SFLRAFK+S PDSF  + + RYG
Sbjct: 24  WLVRKVNVWLYES-SLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYG 83

Query: 78  RTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKR 137
              +YK  MFG+PS+IVT+++ C+RVL DDDAF  GWP ST++LIG KSF+GIS +EHKR
Sbjct: 84  PKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKR 143

Query: 138 LRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSS 197
           LRR+TAAPVNG+EAL+TY+ YIE   +  L KW  MG+ EFLT LRKLTF+IIMYIFLSS
Sbjct: 144 LRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLSS 203

Query: 198 ESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDND 257
           ESE VM+ALEREYT LNYGVRAMA+NIPGFAYH+ALKARK LV  FQSIV++RR +R  +
Sbjct: 204 ESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 263

Query: 258 VATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNF 317
           + + ++DM+D LL+V+DE+G+ L DEEIIDVL+MYLNAGHESSGHT+MWAT+FLQ+HP  
Sbjct: 264 ILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 323

Query: 318 LGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNI 377
           L +AKAEQE ILK RP GQKGL+LKE R+M++LS+V+DETLRV+TFSLT FREAK DV +
Sbjct: 324 LQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEM 383

Query: 378 CGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDD-FTPKAGSFLPFGGGSRLCPGN 437
            GY IPKGWKVL WFR VH DPE +P+P++FDP+RWD+ F PKAG+FLPFG GS LCPGN
Sbjct: 384 NGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGN 443

Query: 438 DLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARI 482
           DLAKLEIS+FLH+FLL Y+++R NP  PV YLPHTRP D CLARI
Sbjct: 444 DLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Carg11066 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.3e-166
Identity = 280/488 (57.38%), Postives = 359/488 (73.57%), Query Frame = 0

Query: 4   AVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSS 63
           A + AAL  VA     ++ A+ W         +R  LPPGD+GWP +G MW+FLRAFKS 
Sbjct: 10  AAVVAALAVVALDA-AVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSG 69

Query: 64  RPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIG 123
           RPDSF++SF  R+GR  +Y+ FMF SP+++ T+ EACK+VL DDDAF +GWP++T+ LIG
Sbjct: 70  RPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIG 129

Query: 124 EKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSM---GQIEFLT 183
            KSF+ +   EH+RLR++TAAP+NG++AL +YL +I++  V +L+ W      G  EFLT
Sbjct: 130 PKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLT 189

Query: 184 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 243
           ELR++TF+II+ IF+    E     LER YT LNYG+RAMAI++PGFAYHKA++AR+ LV
Sbjct: 190 ELRRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLV 249

Query: 244 MTFQSIVSQRRKERDNDVATTRR--DMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHE 303
              Q ++ +RR       A      DMMD L+ VEDE GR L D+EIIDVL+MYLNAGHE
Sbjct: 250 AALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHE 309

Query: 304 SSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETL 363
           SSGH  MWAT+FLQ++P  L KAKAEQE I++  PPGQKGLTL++ R+M YLS+V+DETL
Sbjct: 310 SSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETL 369

Query: 364 RVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTP 423
           R V  S   FR+A  DV + GY IPKGWKV +W+RSVH DP+ YP+PK+FDPSRW+   P
Sbjct: 370 RFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPP 429

Query: 424 KAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCL 483
           +AG+FLPFG G+RLCPGNDLAKLEISVFLH+FLL Y+L R NPN  VRYLPH RP D CL
Sbjct: 430 RAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHPRPVDNCL 489

Query: 484 ARIKRVGA 487
           A+I R+ +
Sbjct: 490 AKITRLSS 496

BLAST of Carg11066 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 9.6e-166
Identity = 276/489 (56.44%), Postives = 361/489 (73.82%), Query Frame = 0

Query: 2   YTAVLAAALVAVAATVWV---LKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLR 61
           Y     A LV   A V V    + A+ W  E     ++R  LPPG++GWP +G MW+FLR
Sbjct: 31  YREAAEAVLVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLR 90

Query: 62  AFKSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRST 121
           AFKS +PD+F+ SFV R+GRT +Y+ FMF SP+V+VT+AE CK+VL DDDAF +GWP++T
Sbjct: 91  AFKSGKPDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKAT 150

Query: 122 LKLIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSM-GQIE 181
           + L+G +SF+ +   EH+R+R++TAAP+NG++AL  YL +I+    +SL+ W    G +E
Sbjct: 151 VALVGPRSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVE 210

Query: 182 FLTELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARK 241
           FLTELR++TFKII+ IFL    +    ALER YT LNYG+RAMAIN+PGFAY  AL+AR+
Sbjct: 211 FLTELRRMTFKIIVQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARR 270

Query: 242 NLVMTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGH 301
            LV   Q ++ +RR  R   V+    DMMD L++ +DE GR L D+EIIDVL+MYLNAGH
Sbjct: 271 RLVAVLQGVLDERRAARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGH 330

Query: 302 ESSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDET 361
           ESSGH  MWAT+FLQ++P+   +AKAEQE I++  P  Q+GLTL++ R+M+YLS+VIDET
Sbjct: 331 ESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDET 390

Query: 362 LRVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFT 421
           LR+V  S   FR+A  DV + GY IPKGWKV +W+RSVH DP+ YP+P +FDPSRW+  +
Sbjct: 391 LRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHS 450

Query: 422 PKAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQC 481
           P+AG+FL FG G+RLCPGNDLAKLEISVFLH+FLL Y+L R NP   VRYLPH RP D C
Sbjct: 451 PRAGTFLAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNC 510

Query: 482 LARIKRVGA 487
           LA+I RVG+
Sbjct: 511 LAKITRVGS 519

BLAST of Carg11066 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 9.6e-166
Identity = 275/484 (56.82%), Postives = 366/484 (75.62%), Query Frame = 0

Query: 5   VLAAALVA-VAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSS 64
           VLAAA+VA V     V++ A+ W+        +R  LPPG++GWP +G+MW+FLRAFKS 
Sbjct: 19  VLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSG 78

Query: 65  RPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIG 124
            PD+F+ SF+ R+GRT +Y+ FMF SP+++  + EACK+VL DD+ F +GWP++T+ LIG
Sbjct: 79  NPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIG 138

Query: 125 EKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKW--PSMGQIEFLTE 184
            KSF+ +S  +H+R+R++TAAP+NG++AL TYL++I+   VASL++W  P  GQ+EFLTE
Sbjct: 139 PKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTE 198

Query: 185 LRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVM 244
           LR++TFKII+ IF+S   +  MEALER YT LNYG+RAMAIN+PGFAY++AL+AR+ LV 
Sbjct: 199 LRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVS 258

Query: 245 TFQSIVSQRRKERDNDVATT-RRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 304
             Q ++  RR         +   DMMD L++ EDE GR L+D+EI+DVLIMYLNAGHESS
Sbjct: 259 VLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESS 318

Query: 305 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 364
           GH  MWAT+FLQ++P+   +AKAEQEEI++  P  Q GLTL++ ++M +LS+V+DETLR 
Sbjct: 319 GHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRC 378

Query: 365 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 424
           V  S   FR+A  D+ + GY IPKGWKV +W+RSVH D + YP+PK F+PSRW+   PKA
Sbjct: 379 VNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKA 438

Query: 425 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 484
           G+FLPFG G+RLCPGNDLAKLEISVFLH+FLL Y+L+R NP   VRYLPH RP D CLA 
Sbjct: 439 GTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLAT 498

BLAST of Carg11066 vs. ExPASy TrEMBL
Match: A0A6J1HJH6 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464585 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.2e-285
Identity = 486/489 (99.39%), Postives = 487/489 (99.59%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTS EACKRVLNDDDAFGSGWPRSTLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSTEACKRVLNDDDAFGSGWPRSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
           MTFQSIVSQRRK+RDNDVATTRRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESS
Sbjct: 241 MTFQSIVSQRRKKRDNDVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV
Sbjct: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVGAPFD
Sbjct: 481 IKRVGAPFD 489

BLAST of Carg11066 vs. ExPASy TrEMBL
Match: A0A6J1KIC3 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111494707 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.0e-279
Identity = 476/489 (97.34%), Postives = 483/489 (98.77%), Query Frame = 0

Query: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60
           MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF
Sbjct: 1   MYTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAF 60

Query: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLK 120
           KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWP STLK
Sbjct: 61  KSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPHSTLK 120

Query: 121 LIGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLT 180
           LIGEKSFIGIS+KEHKRLRRITAAPVNGYEALATYLTYIEN+TV+SLQKWPSMGQIEFLT
Sbjct: 121 LIGEKSFIGISAKEHKRLRRITAAPVNGYEALATYLTYIENVTVSSLQKWPSMGQIEFLT 180

Query: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240
           ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV
Sbjct: 181 ELRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLV 240

Query: 241 MTFQSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 300
            TFQSIVSQRRKERDN+VATTRRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESS
Sbjct: 241 TTFQSIVSQRRKERDNNVATTRRDMMDALLDVEDENGRRLSDEEIIDVLIMYLNAGHESS 300

Query: 301 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 360
           GHTMMWATIFLQQHPNFL KAKAEQEEILKKR PGQKGLTLKEVREMDYLSKVIDETLR+
Sbjct: 301 GHTMMWATIFLQQHPNFLEKAKAEQEEILKKRSPGQKGLTLKEVREMDYLSKVIDETLRI 360

Query: 361 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420
           VTFSL VFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA
Sbjct: 361 VTFSLMVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 420

Query: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 480
           GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNY+LERDNPNSPVRYLPH+RPKDQCLAR
Sbjct: 421 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYQLERDNPNSPVRYLPHSRPKDQCLAR 480

Query: 481 IKRVGAPFD 490
           IKRVGAPFD
Sbjct: 481 IKRVGAPFD 489

BLAST of Carg11066 vs. ExPASy TrEMBL
Match: A0A6J1DNE5 (ent-kaurenoic acid oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111022826 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.1e-236
Identity = 402/482 (83.40%), Postives = 431/482 (89.42%), Query Frame = 0

Query: 4   AVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSS 63
           A    A  AVAA VWV KNANRW++E  RLG KRF LPPGDLGWPF+GNMW FLRAFKSS
Sbjct: 5   AAWVVAAAAVAAAVWVAKNANRWVYENRRLGEKRFWLPPGDLGWPFVGNMWDFLRAFKSS 64

Query: 64  RPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIG 123
            PDSFM SFV+RYGR  +YK  MFGSPSV+V+SAEACKRVL DDDAFGSGWP ST+KLIG
Sbjct: 65  DPDSFMASFVSRYGRRGIYKSLMFGSPSVLVSSAEACKRVLTDDDAFGSGWPVSTMKLIG 124

Query: 124 EKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELR 183
           EKSFIGIS +EHKRLRRITAAPVNGYEALATYL YIE   V SLQKW +MGQIEFLT LR
Sbjct: 125 EKSFIGISYQEHKRLRRITAAPVNGYEALATYLKYIEQTVVVSLQKWATMGQIEFLTHLR 184

Query: 184 KLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTF 243
           KLTF IIM+IFLSSESE VM+A+EREYT LNYGVRAMAINIPGFAYHKALKAR+NLV TF
Sbjct: 185 KLTFNIIMHIFLSSESETVMDAMEREYTKLNYGVRAMAINIPGFAYHKALKARRNLVATF 244

Query: 244 QSIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHT 303
           QSIV+ RRKER+N++AT RRDMMDALLDVEDENGR LSDEEIIDVLIMYLNAGHESSGHT
Sbjct: 245 QSIVTNRRKERENNLATKRRDMMDALLDVEDENGRKLSDEEIIDVLIMYLNAGHESSGHT 304

Query: 304 MMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTF 363
           MMWATIFLQQHP F  KAK EQEEIL++RP  QKGLTLKEVREM YLSKVIDETLRVVTF
Sbjct: 305 MMWATIFLQQHPEFFEKAKIEQEEILRRRPASQKGLTLKEVREMYYLSKVIDETLRVVTF 364

Query: 364 SLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSF 423
           SL VFREAKHDVNI GY IPKGWK+LVWFR VH DP+TYPNPK+F+PSRWDDFTPKAGSF
Sbjct: 365 SLMVFREAKHDVNISGYLIPKGWKILVWFRGVHLDPQTYPNPKEFNPSRWDDFTPKAGSF 424

Query: 424 LPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKR 483
           LPFG GSRLCPGNDLAKLE+S+FLHYFLLNY+LER NPNSPVRYLPH+RPKD CLAR+KR
Sbjct: 425 LPFGAGSRLCPGNDLAKLEVSIFLHYFLLNYQLERANPNSPVRYLPHSRPKDNCLARVKR 484

Query: 484 VG 486
           VG
Sbjct: 485 VG 486

BLAST of Carg11066 vs. ExPASy TrEMBL
Match: A0A2P5C6Q9 (Cytochrome P450 OS=Parasponia andersonii OX=3476 GN=PanCYP21 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.4e-214
Identity = 367/483 (75.98%), Postives = 419/483 (86.75%), Query Frame = 0

Query: 2   YTAVLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFK 61
           +  VL   L +V    W+LKN N W++ET +LG K+F LPPGDLGWPF+GNMWSFLRAFK
Sbjct: 10  WVVVLFWFLGSVVVLRWLLKNVNWWLYET-KLGEKQFFLPPGDLGWPFLGNMWSFLRAFK 69

Query: 62  SSRPDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKL 121
           SS PDSF+ SFV+R+GRT +YK FMFG+PS+IVT+ E CKRVL DDDAF  GWPRST++L
Sbjct: 70  SSDPDSFISSFVSRFGRTGIYKAFMFGNPSIIVTTPETCKRVLTDDDAFKPGWPRSTVEL 129

Query: 122 IGEKSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTE 181
           IG+KSFIGIS +EHKRL+R+TAAPVNG+EAL+ Y+ YIE   VASL+KW +MGQIEFLTE
Sbjct: 130 IGKKSFIGISFEEHKRLKRLTAAPVNGHEALSLYIPYIEEKVVASLEKWSNMGQIEFLTE 189

Query: 182 LRKLTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVM 241
           LRKLTF+IIMYIFLSSESE VMEALEREYT LNYGVRAM+INIPGFAYHKALKAR+ LV 
Sbjct: 190 LRKLTFRIIMYIFLSSESEPVMEALEREYTVLNYGVRAMSINIPGFAYHKALKARRKLVA 249

Query: 242 TFQSIVSQRRKER-DNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESS 301
            FQSIV +RR +R DN V T ++DMMDALLDVEDE+GR L+DEEIIDVL+MYLNAGHESS
Sbjct: 250 VFQSIVEERRFQRKDNLVPTKKKDMMDALLDVEDESGRKLTDEEIIDVLLMYLNAGHESS 309

Query: 302 GHTMMWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRV 361
           GHTMMW TIFL+QHP+ L KAK EQE I+KKRPP QKGLTLKE+REM YLS VIDETLRV
Sbjct: 310 GHTMMWTTIFLEQHPDILQKAKEEQEGIVKKRPPTQKGLTLKEIREMKYLSDVIDETLRV 369

Query: 362 VTFSLTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKA 421
           +TFSLTVFREAK DVNI GYTIPKGWKVLVWFRSVH DPE YPNP +F+PSRW+ +TPKA
Sbjct: 370 ITFSLTVFREAKRDVNISGYTIPKGWKVLVWFRSVHLDPEIYPNPSEFNPSRWNGYTPKA 429

Query: 422 GSFLPFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLAR 481
           GSFLPFG GSRLCPGNDLAKLEIS+FLHYFLLNYR+++ NP S V YLPH RPKD CLA+
Sbjct: 430 GSFLPFGAGSRLCPGNDLAKLEISIFLHYFLLNYRVKQANPGSQVMYLPHCRPKDNCLAK 489

Query: 482 IKR 484
           I++
Sbjct: 490 IEK 491

BLAST of Carg11066 vs. ExPASy TrEMBL
Match: F6KWJ3 (Ent-kaurenoic acid oxidase OS=Castanea mollissima OX=60419 GN=KAO PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.7e-214
Identity = 363/479 (75.78%), Postives = 413/479 (86.22%), Query Frame = 0

Query: 5   VLAAALVAVAATVWVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSR 64
           VL      +    WV+KNAN W++ET +LG K+++LPPGDLGWPFIGNMWSFL AFKS  
Sbjct: 9   VLLCIFGVLVVLKWVVKNANWWLYET-QLGEKQYSLPPGDLGWPFIGNMWSFLSAFKSKD 68

Query: 65  PDSFMESFVARYGRTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGE 124
           PDSF+ SFV+R+GRT +YK FMFG+PSVIVTS EAC+RVL+DDD F  GWP+ST+ LIG+
Sbjct: 69  PDSFVSSFVSRFGRTGIYKVFMFGNPSVIVTSPEACRRVLSDDDCFKPGWPKSTVALIGK 128

Query: 125 KSFIGISSKEHKRLRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRK 184
           KSFIGIS +EHKRLRR+TAAPVNGYEAL+ Y  YIE +  +SL KW +MG+IEFLTELRK
Sbjct: 129 KSFIGISFEEHKRLRRLTAAPVNGYEALSMYTKYIEEIVTSSLDKWTTMGEIEFLTELRK 188

Query: 185 LTFKIIMYIFLSSESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQ 244
           LTF+IIMYIFLSSESE VM ALEREYT LNYGVRAMAINIPGFAYHKALKARKNLV TFQ
Sbjct: 189 LTFRIIMYIFLSSESEPVMTALEREYTALNYGVRAMAINIPGFAYHKALKARKNLVATFQ 248

Query: 245 SIVSQRRKERDNDVATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTM 304
           SIV  RR ++   V T ++DMMDAL+DV+DENGR L+DEEIID+L+MYLNAGHESSGHT 
Sbjct: 249 SIVDDRRNQKKVSVPTKKKDMMDALIDVKDENGRKLTDEEIIDILVMYLNAGHESSGHTA 308

Query: 305 MWATIFLQQHPNFLGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFS 364
           MWATIFL QHP +L KAK EQEEI++KR P QKGLTLKE+REM+YLSKVIDETLRV+TFS
Sbjct: 309 MWATIFLNQHPEYLQKAKKEQEEIVRKRLPEQKGLTLKEIREMEYLSKVIDETLRVITFS 368

Query: 365 LTVFREAKHDVNICGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFL 424
           LTVFREAK DVNI GYTIPKGW+VLVWFRSVH DPE Y NPK+F PSRWD+FTPKAG+FL
Sbjct: 369 LTVFREAKQDVNIAGYTIPKGWRVLVWFRSVHLDPEIYENPKEFKPSRWDNFTPKAGAFL 428

Query: 425 PFGGGSRLCPGNDLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKR 484
           PFG G+RLCPGNDLAKLEIS+FLH+FLLNY+LER NP SP  YLPHTRP D CLARIK+
Sbjct: 429 PFGAGTRLCPGNDLAKLEISIFLHHFLLNYQLERLNPGSPSMYLPHTRPVDNCLARIKK 486

BLAST of Carg11066 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 690.6 bits (1781), Expect = 8.8e-199
Identity = 327/466 (70.17%), Postives = 387/466 (83.05%), Query Frame = 0

Query: 18  WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYG 77
           WVLK  N WI+ + +LG K+  LPPGDLGWP IGNMWSFLRAFK+S P+SF++S++ RYG
Sbjct: 23  WVLKRVNVWIYVS-KLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYG 82

Query: 78  RTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKR 137
           RT +YK  MFG P V+VT+ E C+RVL DDDAF  GWP+ST+KLIG KSF+GIS +EHKR
Sbjct: 83  RTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKR 142

Query: 138 LRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSS 197
           LRR+T+APVNG EAL+ Y+ +IE      L+KW  MG+IEFL+ LRKLTFK+IMYIFLSS
Sbjct: 143 LRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSS 202

Query: 198 ESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDND 257
           ESE VM++LEREYT LNYGVRAM IN+PGFAYH+ALKARK LV  FQSIV+ RR +R  +
Sbjct: 203 ESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 262

Query: 258 VATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNF 317
           +++ R+DM+D L+DV+DENGR+L DEEIID+L+MYLNAGHESSGH  MWATI +Q+HP  
Sbjct: 263 ISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMI 322

Query: 318 LGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNI 377
           L KAK EQE I+KKR PGQK LTLKE REM YLS+VIDETLRV+TFSLT FREAK DV +
Sbjct: 323 LQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQM 382

Query: 378 CGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGND 437
            GY IPKGWKVL WFR+VH DPE YP+PK+FDPSRW+ +TPKAG+FLPFG GS LCPGND
Sbjct: 383 DGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGND 442

Query: 438 LAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKR 484
           LAKLEIS+FLH+FLL YR+ER NP  PV +LPH RPKD CLARI R
Sbjct: 443 LAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of Carg11066 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 690.6 bits (1781), Expect = 8.8e-199
Identity = 327/466 (70.17%), Postives = 387/466 (83.05%), Query Frame = 0

Query: 18  WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYG 77
           WVLK  N WI+ + +LG K+  LPPGDLGWP IGNMWSFLRAFK+S P+SF++S++ RYG
Sbjct: 23  WVLKRVNVWIYVS-KLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFIQSYITRYG 82

Query: 78  RTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKR 137
           RT +YK  MFG P V+VT+ E C+RVL DDDAF  GWP+ST+KLIG KSF+GIS +EHKR
Sbjct: 83  RTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKR 142

Query: 138 LRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSS 197
           LRR+T+APVNG EAL+ Y+ +IE      L+KW  MG+IEFL+ LRKLTFK+IMYIFLSS
Sbjct: 143 LRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLTFKVIMYIFLSS 202

Query: 198 ESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDND 257
           ESE VM++LEREYT LNYGVRAM IN+PGFAYH+ALKARK LV  FQSIV+ RR +R  +
Sbjct: 203 ESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 262

Query: 258 VATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNF 317
           +++ R+DM+D L+DV+DENGR+L DEEIID+L+MYLNAGHESSGH  MWATI +Q+HP  
Sbjct: 263 ISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMI 322

Query: 318 LGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNI 377
           L KAK EQE I+KKR PGQK LTLKE REM YLS+VIDETLRV+TFSLT FREAK DV +
Sbjct: 323 LQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQM 382

Query: 378 CGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGND 437
            GY IPKGWKVL WFR+VH DPE YP+PK+FDPSRW+ +TPKAG+FLPFG GS LCPGND
Sbjct: 383 DGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGND 442

Query: 438 LAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARIKR 484
           LAKLEIS+FLH+FLL YR+ER NP  PV +LPH RPKD CLARI R
Sbjct: 443 LAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of Carg11066 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 682.2 bits (1759), Expect = 3.1e-196
Identity = 319/465 (68.60%), Postives = 387/465 (83.23%), Query Frame = 0

Query: 18  WVLKNANRWIHETFRLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYG 77
           W+++  N W++E+  LG  R  LPPGDLGWPFIGNM SFLRAFK+S PDSF  + + RYG
Sbjct: 24  WLVRKVNVWLYES-SLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYG 83

Query: 78  RTDMYKCFMFGSPSVIVTSAEACKRVLNDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKR 137
              +YK  MFG+PS+IVT+++ C+RVL DDDAF  GWP ST++LIG KSF+GIS +EHKR
Sbjct: 84  PKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKR 143

Query: 138 LRRITAAPVNGYEALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSS 197
           LRR+TAAPVNG+EAL+TY+ YIE   +  L KW  MG+ EFLT LRKLTF+IIMYIFLSS
Sbjct: 144 LRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTFRIIMYIFLSS 203

Query: 198 ESEVVMEALEREYTTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDND 257
           ESE VM+ALEREYT LNYGVRAMA+NIPGFAYH+ALKARK LV  FQSIV++RR +R  +
Sbjct: 204 ESENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 263

Query: 258 VATTRRDMMDALLDVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNF 317
           + + ++DM+D LL+V+DE+G+ L DEEIIDVL+MYLNAGHESSGHT+MWAT+FLQ+HP  
Sbjct: 264 ILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 323

Query: 318 LGKAKAEQEEILKKRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNI 377
           L +AKAEQE ILK RP GQKGL+LKE R+M++LS+V+DETLRV+TFSLT FREAK DV +
Sbjct: 324 LQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEM 383

Query: 378 CGYTIPKGWKVLVWFRSVHYDPETYPNPKQFDPSRWDD-FTPKAGSFLPFGGGSRLCPGN 437
            GY IPKGWKVL WFR VH DPE +P+P++FDP+RWD+ F PKAG+FLPFG GS LCPGN
Sbjct: 384 NGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGN 443

Query: 438 DLAKLEISVFLHYFLLNYRLERDNPNSPVRYLPHTRPKDQCLARI 482
           DLAKLEIS+FLH+FLL Y+++R NP  PV YLPHTRP D CLARI
Sbjct: 444 DLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Carg11066 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-70
Identity = 155/451 (34.37%), Postives = 243/451 (53.88%), Query Frame = 0

Query: 35  SKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYGRTDMYKCFMFGSPSVIV 94
           + R  LPPG +GWP++G          S  P+ F  S   RYG  +++K  + G P V++
Sbjct: 29  NSRGKLPPGSMGWPYLGETLQLY----SQNPNVFFTSKQKRYG--EIFKTRILGYPCVML 88

Query: 95  TSAEACKRVL-NDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKRLRRITAAPVNGYEALA 154
            S EA + VL      F   +PRS  KLIG  +        H  +R++  +     E + 
Sbjct: 89  ASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYP-ETIR 148

Query: 155 TYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKI-IMYIFLSSESEVVMEALEREYTT 214
             +  IE++ ++SLQ W +M  +    E++K  F + I+ IF   ES    E L+  Y  
Sbjct: 149 KLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLESS-YKEILKHNYNI 208

Query: 215 LNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDNDVATTRRDMMDALLDV 274
           ++ G  +  +++PG +YHKAL ARK L      I+ +RR++R       + D +  LL+ 
Sbjct: 209 VDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKR-----ALQTDFLGHLLNF 268

Query: 275 EDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNFLGKAKAEQEEILKKR 334
           ++E GR+L+ E+I D +I  L A  +++   + W   +L      L   KAEQ+ I ++ 
Sbjct: 269 KNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEEN 328

Query: 335 PPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNICGYTIPKGWKVLVWF 394
              +K LT ++ R M    KVI E+LR+ +     FREA  DV   GY IPKGWKV+  F
Sbjct: 329 SREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLF 388

Query: 395 RSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGNDLAKLEISVFLHYFLL 454
           R++H++P+ + NP+ FDPSR+ +  PK  +F+PFG G   CPGN+LAKL+I +FLH+ + 
Sbjct: 389 RNIHHNPKYFSNPEVFDPSRF-EVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVS 448

Query: 455 NYRLERDNPNSPVRYLPHTRPKDQCLARIKR 484
           N+R E       ++Y P   P++   A  +R
Sbjct: 449 NFRWEVKGGEKGIQYSPFPIPQNGLPATFRR 465

BLAST of Carg11066 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 148/448 (33.04%), Postives = 238/448 (53.12%), Query Frame = 0

Query: 32  RLGSKRFNLPPGDLGWPFIGNMWSFLRAFKSSRPDSFMESFVARYGRTDMYKCFMFGSPS 91
           R GS +  LPPG +GWP++G  +       S  P+ F +S   RYG   ++K  + G P 
Sbjct: 28  RFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFFQSKQKRYG--SVFKTHVLGCPC 87

Query: 92  VIVTSAEACKRVL-NDDDAFGSGWPRSTLKLIGEKSFIGISSKEHKRLRRITAAPVNGYE 151
           V+++S EA K VL      F   +P S  +++G+++        H +LR++        E
Sbjct: 88  VMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMP-E 147

Query: 152 ALATYLTYIENMTVASLQKWPSMGQIEFLTELRKLTFKIIMYIFLSSESEVVMEALEREY 211
           ++   +  IE++   SL+ W     I    E++  TF + +      +  +  E L+R Y
Sbjct: 148 SIRNMVPDIESIAQDSLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYREDLKRCY 207

Query: 212 TTLNYGVRAMAINIPGFAYHKALKARKNLVMTFQSIVSQRRKERDNDVATTRRDMMDALL 271
             L  G  +M +N+PG  +HK++KARK L      I+S+RR+       ++  D++ + +
Sbjct: 208 YILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQN-----GSSHNDLLGSFM 267

Query: 272 DVEDENGRMLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPNFLGKAKAEQEEILK 331
             ++E    L+DE+I D +I  + A  +++   M W   +L ++PN L     EQ  I K
Sbjct: 268 GDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRK 327

Query: 332 KRPPGQKGLTLKEVREMDYLSKVIDETLRVVTFSLTVFREAKHDVNICGYTIPKGWKVLV 391
            +  G+  LT  + ++M   S+VI ETLRV +     FREA  DV   GY IPKGWKVL 
Sbjct: 328 DKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLP 387

Query: 392 WFRSVHYDPETYPNPKQFDPSRWDDFTPKAGSFLPFGGGSRLCPGNDLAKLEISVFLHYF 451
            FR++H+  + + NP +FDPSR+ +  PK  +F+PFG G+  CPGN+LAKLE+S+ +H+ 
Sbjct: 388 LFRNIHHSADIFSNPGKFDPSRF-EVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHL 447

Query: 452 LLNYRLERDNPNSPVRYLPHTR-PKDQC 478
              YR  +    +    + H+R PK  C
Sbjct: 448 TTKYRFGQLLERATGFSMGHSRFPKTDC 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6593731.13.1e-286100.00Ent-kaurenoic acid oxidase 2, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022964631.11.3e-28499.39ent-kaurenoic acid oxidase 2-like [Cucurbita moschata][more]
XP_023513658.13.2e-28398.77ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo][more]
XP_023000440.16.2e-27997.34ent-kaurenoic acid oxidase 2-like [Cucurbita maxima][more]
XP_038907052.13.4e-23784.57ent-kaurenoic acid oxidase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9C5Y21.2e-19770.17Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
O230514.4e-19568.60Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9AXH93.3e-16657.38Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q432469.6e-16656.44Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Q5VRM79.6e-16656.82Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HJH66.2e-28599.39ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464585 ... [more]
A0A6J1KIC33.0e-27997.34ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111494707 PE... [more]
A0A6J1DNE54.1e-23683.40ent-kaurenoic acid oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111022826... [more]
A0A2P5C6Q94.4e-21475.98Cytochrome P450 OS=Parasponia andersonii OX=3476 GN=PanCYP21 PE=3 SV=1[more]
F6KWJ35.7e-21475.78Ent-kaurenoic acid oxidase OS=Castanea mollissima OX=60419 GN=KAO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G32440.18.8e-19970.17ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.28.8e-19970.17ent-kaurenoic acid hydroxylase 2 [more]
AT1G05160.13.1e-19668.60cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT3G19270.15.9e-7034.37cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.23.0e-6633.04cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 433..444
score: 39.45
coord: 352..363
score: 34.95
coord: 295..312
score: 26.29
coord: 424..433
score: 58.22
IPR001128Cytochrome P450PFAMPF00067p450coord: 41..461
e-value: 4.2E-68
score: 230.2
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 304..330
score: 21.27
coord: 433..456
score: 31.85
coord: 391..415
score: 29.44
coord: 423..433
score: 54.62
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 31..484
e-value: 7.6E-107
score: 359.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..482
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 12..477
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 12..477
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 426..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11066-RACarg11066-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051777 ent-kaurenoate oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen