Carg11032 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11032
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr08: 5978241 .. 5979329 (-)
RNA-Seq ExpressionCarg11032
SyntenyCarg11032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAAGTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGTGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

mRNA sequence

ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAAGTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGTGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

Coding sequence (CDS)

ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAAGTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGTGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

Protein sequence

MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNSPN
Homology
BLAST of Carg11032 vs. NCBI nr
Match: KAG7026103.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 718.8 bits (1854), Expect = 2.4e-203
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of Carg11032 vs. NCBI nr
Match: XP_022964349.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 361/362 (99.72%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of Carg11032 vs. NCBI nr
Match: KAG6593770.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714.9 bits (1844), Expect = 3.5e-202
Identity = 360/362 (99.45%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYC+RSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCQRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of Carg11032 vs. NCBI nr
Match: XP_023000254.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 694.5 bits (1791), Expect = 4.8e-196
Identity = 352/362 (97.24%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSV DGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQGDGRTERREES+HSIRSVSEDHHSSPCPGENLSVADVL+IN
Sbjct: 241 TNQEQEN----PNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKN NS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNPNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of Carg11032 vs. NCBI nr
Match: XP_023513580.1 (RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.8 bits (1784), Expect = 3.1e-195
Identity = 350/362 (96.69%), Postives = 354/362 (97.79%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENE+LRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENENLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSD AVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDTAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQG GRTERREES+HSIRSVSEDHHS+PCPGENLSVADVLNIN
Sbjct: 241 TNQEQEN----PNPRSFDQGHGRTERREESIHSIRSVSEDHHSAPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSN NNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNANNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of Carg11032 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.8e-60
Identity = 147/368 (39.95%), Postives = 213/368 (57.88%), Query Frame = 0

Query: 12  PFNPF-NFKDCSQQSCTAFCPQWCAFLFPPPPPFFGL-DDSDDSSTFFSPLIVAVIGILA 71
           P++P+ ++ DCSQ  C  +CPQWC  +FPPPPP F L DDS  SS+ FSPL++A+IGIL 
Sbjct: 7   PWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILT 66

Query: 72  SAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRR-SSAGSGLDEALIKSIS 131
           SA +LV+YY ++SKYC R            +       + T+R S+ G GL+E++IKSI+
Sbjct: 67  SALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSIT 126

Query: 132 VYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSN 191
           VYK+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKS+S+CPLCR+ 
Sbjct: 127 VYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAF 186

Query: 192 IASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQ--------ESVVV--EIVS 251
           +   N P           +  + +S      V +++ N++         +SVVV  ++  
Sbjct: 187 VTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEI 246

Query: 252 DSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLN 311
            S N +  +    P  R       R  + E+S+   RSVS +       G  +S+ADVL 
Sbjct: 247 GSRNSDARSELQLPEER-------RETKDEDSLPIRRSVSLN------SGVVVSIADVLR 306

Query: 312 INHEEDEEIEALDSSMAA-----GIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYT 362
              +E+ E   + +S        G G +    +  ++S   +G    S+S+G F F+RY 
Sbjct: 307 EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGRFIFSRYD 361

BLAST of Carg11032 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 2.7e-56
Identity = 141/369 (38.21%), Postives = 202/369 (54.74%), Query Frame = 0

Query: 17  NFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVT 76
           +++DCSQ  C+ +CPQWC  +FPPPP F+  D+ D SS+ FSPL++A+IGILASAF+LV+
Sbjct: 16  SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVS 75

Query: 77  YYAIVSKYCRRSVGDG------------------GGGGPEESFETNRAINETRRSSAGSG 136
           YY ++SKYC R   +                   G      +  TN   N+T     G G
Sbjct: 76  YYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNP--NQTIGGGGGDG 135

Query: 137 LDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKS 196
           LDE+LIKSI+VYK++K +G +E SDCSVCLSEFQENESLRLLPKC+HAFH+PCIDTWLKS
Sbjct: 136 LDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 195

Query: 197 NSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVV---E 256
           +S+CPLCR+ I +++          V  +   +      +V ++ S +   +SVVV   +
Sbjct: 196 HSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENNSISTGDDSVVVVNLD 255

Query: 257 IVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVAD 316
           + +  +  E  N   +P P          ERR  S++S              G  +S+AD
Sbjct: 256 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNS-------------GGGVVSIAD 315

Query: 317 VLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNK-NGVLRMSISSGIFSFTR--Y 362
           +L    E+DEE   + +S     G        +  + N+ NG+    + S + +  R  Y
Sbjct: 316 ILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMKRSGY 355

BLAST of Carg11032 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 2.2e-50
Identity = 139/361 (38.50%), Postives = 191/361 (52.91%), Query Frame = 0

Query: 6   ISDPSPPFNPFN-FKDCSQQSCTAFCPQWCAFLFPPPPPF--FGLDDSDDSSTFFSPLIV 65
           ++  +P + P+   +DCSQ  C+ +CPQWC F+FPPPPPF   G    D S   FSPL++
Sbjct: 1   MASSAPAWVPYEPTRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVI 60

Query: 66  AVIGILASAFVLVTYYAIVSKYC----------------RRSVGDGGGGGPEESFETNRA 125
           A+IG+LASAF+LV+YY  +SKYC                  + G G G G       +R+
Sbjct: 61  AIIGVLASAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRS 120

Query: 126 INETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHA 185
            +E+   S  SGLDE LI  I+V K+++G+G +  +DCSVCL EF + ESLRLLP+CSHA
Sbjct: 121 -HESWNVSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHA 180

Query: 186 FHLPCIDTWLKSNSSCPLCRSNIASTN----PPPQETPPPPVTALGYRHSSDAAVVVVQD 245
           FH  CIDTWLKS+S+CPLCR+NI         P  E   P  T     H     VVVV D
Sbjct: 181 FHQQCIDTWLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHE----VVVVMD 240

Query: 246 LSANVSQESVVVEIVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSE-DH 305
              N+ +E    E VS ++  + ++ +        ++ +G  E REE     R  S  D 
Sbjct: 241 GLENLCEEQ--QEAVSRASTADDDH-DAKDVAEGMEEANGAAEIREEGSPPKRGASSFDL 300

Query: 306 HSSPCPGENLSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMS 343
           H        + +ADVL     EDE   A +S + AG G         E S+ + G  R +
Sbjct: 301 HRD----NRMCIADVLQ-ESMEDELTAARESGLLAG-GAGTSRRCHGENSKGRGGRSRRA 347

BLAST of Carg11032 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 3.5e-40
Identity = 92/204 (45.10%), Postives = 127/204 (62.25%), Query Frame = 0

Query: 7   SDPSP-PFNPF-NFKDCSQQSCTAFCPQWCAFL-FPPPPPFFG--LDDSDDSSTFFSPLI 66
           SDP+P  +N + N +DC+Q  C+ FCPQWC ++ F PPP  +   L+D   S+   SPL+
Sbjct: 3   SDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLV 62

Query: 67  VAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRA-----INETRR---- 126
           +A+ GI A+AF+L  YY +VSKYC     +      E + E+ R+     +N   R    
Sbjct: 63  IAIFGIFATAFLLAAYYTLVSKYCANDTTN------EAASESGRSDIILDVNSPERGDQD 122

Query: 127 -----SSAGSGLDEALIKSISVYKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSH 186
                 S+ +GLD+ LIK I  +K KK +    I G+DCS+CL EF E+ESLRLLPKC+H
Sbjct: 123 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 182

Query: 187 AFHLPCIDTWLKSNSSCPLCRSNI 190
            FH+ CID WLKS+S+CPLCR+ I
Sbjct: 183 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of Carg11032 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 1.3e-26
Identity = 79/223 (35.43%), Postives = 122/223 (54.71%), Query Frame = 0

Query: 3   SIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPP-----FFGLDDSDDSSTFF 62
           S G+ DP  P+N +   D    + +   P W +   P P P        L   D SS+  
Sbjct: 24  SNGVCDPICPYNCYPEPD--YYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 83

Query: 63  SPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGG-----------PEESFETNRA 122
           +  I+ + G + +  +L+T + +V+K+   SV     G             EE F+    
Sbjct: 84  AISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ 143

Query: 123 INETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHA 182
           ++        +GL +++I SI++  +K+G+GLIE +DC VCL+EF+E+ESLRLLPKC+HA
Sbjct: 144 VDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHA 203

Query: 183 FHLPCIDTWLKSNSSCPLCRSNIASTN-PPPQETPPPPVTALG 209
           FH+ CIDTWL S+++CPLCR+ IA  +   P+ + P  VT  G
Sbjct: 204 FHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGG 242

BLAST of Carg11032 vs. ExPASy TrEMBL
Match: A0A6J1HMW7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 5.8e-203
Identity = 361/362 (99.72%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of Carg11032 vs. ExPASy TrEMBL
Match: A0A6J1KJE3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.3e-196
Identity = 352/362 (97.24%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSV DGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQGDGRTERREES+HSIRSVSEDHHSSPCPGENLSVADVL+IN
Sbjct: 241 TNQEQEN----PNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKN NS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNPNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of Carg11032 vs. ExPASy TrEMBL
Match: A0A6J1H2X8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.0e-156
Identity = 290/366 (79.23%), Postives = 316/366 (86.34%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ+ CTAFCPQWCA  F PPPP FGLDDSDDS TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPF-PPPPLFGLDDSDDSDTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R +N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLN 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EAL+KSISV+KFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240

Query: 241 TNQEQ--ENPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVL 300
           TN++Q   NPNP+PNP S D+GDGRTER REES+ SIRSVSEDH SSPCP   LSVAD+L
Sbjct: 241 TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADIL 300

Query: 301 NINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYT 360
           +IN EEDEE+EA++SSMAAG GPSN + KETEKSRN+N VL    RMSI SGIFSF RY 
Sbjct: 301 SINEEEDEEVEAMNSSMAAGSGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYV 360

BLAST of Carg11032 vs. ExPASy TrEMBL
Match: A0A6J1L482 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.1e-153
Identity = 284/364 (78.02%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ+ CTAFCPQWCAF F PPPP FGLDDSD S TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQKLCTAFCPQWCAFPF-PPPPLFGLDDSDKSDTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R  N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVANDTRQSSAGPGLN 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISV+KFKKGEGL+E SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALIKSISVFKFKKGEGLVEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300
           TN++Q +PNP+PNP S D+GDGRTE+ REES+ SIRSVSEDH SSPCP   LSVAD+L+I
Sbjct: 241 TNRDQGSPNPNPNPPSVDRGDGRTEKNREESIRSIRSVSEDHQSSPCPVRILSVADILSI 300

Query: 301 NHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYTKG 360
           + EEDEE+EA++S MAA  GPSN + KETEKSRN+N VL    RMSI SG+FSF RY KG
Sbjct: 301 DEEEDEEVEAMNSPMAADTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGVFSFQRYAKG 360

BLAST of Carg11032 vs. ExPASy TrEMBL
Match: A0A6J1DSH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022765 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.3e-149
Identity = 289/376 (76.86%), Postives = 316/376 (84.04%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFN KDCSQ  CTAFCPQWC ++FPPPPPFFGLDDSDDS TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNLKDCSQGICTAFCPQWCRYVFPPPPPFFGLDDSDDSGTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSV---GDGGGGGPEESFETNRAINETRR-SSAG 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V   G G GGGP + FET+RA+N+ RR S AG
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDSGGGGAGGGPGDYFETDRAMNDARRPSGAG 120

Query: 121 SGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWL 180
            GLDEALIKSISV+KFKK EGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWL
Sbjct: 121 PGLDEALIKSISVFKFKKNEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLVCIDTWL 180

Query: 181 KSNSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEI 240
           KSNSSCPLCR NIASTNP P ETPPP VTALGYRH SDAAVVVVQD S NVSQE+VVVEI
Sbjct: 181 KSNSSCPLCRCNIASTNPNPPETPPPSVTALGYRHRSDAAVVVVQDPSENVSQEAVVVEI 240

Query: 241 ----VSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHS--SPCPGEN 300
               +S+ +NQ+Q   N   NP S +QGDG TERREE+V SIRSVSED  S  SPC G N
Sbjct: 241 IGHSISNPSNQDQGGSN--SNPPSSEQGDGGTERREETVRSIRSVSEDQQSSDSPCLGRN 300

Query: 301 LSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGI 360
           LSVAD+L+I+H+EDEE+EA+DSSMAAGIGPSN + KET KSR +NGVL    RMSI SGI
Sbjct: 301 LSVADILSISHDEDEEVEAVDSSMAAGIGPSNDDGKETGKSRIRNGVLNSAMRMSIPSGI 360

Query: 361 FSFTRYTKGKNSNSPN 363
           FSF ++ KGK+SN PN
Sbjct: 361 FSFAKHAKGKSSNLPN 374

BLAST of Carg11032 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.9e-61
Identity = 147/368 (39.95%), Postives = 213/368 (57.88%), Query Frame = 0

Query: 12  PFNPF-NFKDCSQQSCTAFCPQWCAFLFPPPPPFFGL-DDSDDSSTFFSPLIVAVIGILA 71
           P++P+ ++ DCSQ  C  +CPQWC  +FPPPPP F L DDS  SS+ FSPL++A+IGIL 
Sbjct: 7   PWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILT 66

Query: 72  SAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRR-SSAGSGLDEALIKSIS 131
           SA +LV+YY ++SKYC R            +       + T+R S+ G GL+E++IKSI+
Sbjct: 67  SALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSIT 126

Query: 132 VYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSN 191
           VYK+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKS+S+CPLCR+ 
Sbjct: 127 VYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAF 186

Query: 192 IASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQ--------ESVVV--EIVS 251
           +   N P           +  + +S      V +++ N++         +SVVV  ++  
Sbjct: 187 VTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEI 246

Query: 252 DSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLN 311
            S N +  +    P  R       R  + E+S+   RSVS +       G  +S+ADVL 
Sbjct: 247 GSRNSDARSELQLPEER-------RETKDEDSLPIRRSVSLN------SGVVVSIADVLR 306

Query: 312 INHEEDEEIEALDSSMAA-----GIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYT 362
              +E+ E   + +S        G G +    +  ++S   +G    S+S+G F F+RY 
Sbjct: 307 EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGRFIFSRYD 361

BLAST of Carg11032 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 220.7 bits (561), Expect = 1.9e-57
Identity = 141/369 (38.21%), Postives = 202/369 (54.74%), Query Frame = 0

Query: 17  NFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVT 76
           +++DCSQ  C+ +CPQWC  +FPPPP F+  D+ D SS+ FSPL++A+IGILASAF+LV+
Sbjct: 16  SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVS 75

Query: 77  YYAIVSKYCRRSVGDG------------------GGGGPEESFETNRAINETRRSSAGSG 136
           YY ++SKYC R   +                   G      +  TN   N+T     G G
Sbjct: 76  YYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNP--NQTIGGGGGDG 135

Query: 137 LDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKS 196
           LDE+LIKSI+VYK++K +G +E SDCSVCLSEFQENESLRLLPKC+HAFH+PCIDTWLKS
Sbjct: 136 LDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 195

Query: 197 NSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVV---E 256
           +S+CPLCR+ I +++          V  +   +      +V ++ S +   +SVVV   +
Sbjct: 196 HSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENNSISTGDDSVVVVNLD 255

Query: 257 IVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVAD 316
           + +  +  E  N   +P P          ERR  S++S              G  +S+AD
Sbjct: 256 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNS-------------GGGVVSIAD 315

Query: 317 VLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNK-NGVLRMSISSGIFSFTR--Y 362
           +L    E+DEE   + +S     G        +  + N+ NG+    + S + +  R  Y
Sbjct: 316 ILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMKRSGY 355

BLAST of Carg11032 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 2.5e-41
Identity = 92/204 (45.10%), Postives = 127/204 (62.25%), Query Frame = 0

Query: 7   SDPSP-PFNPF-NFKDCSQQSCTAFCPQWCAFL-FPPPPPFFG--LDDSDDSSTFFSPLI 66
           SDP+P  +N + N +DC+Q  C+ FCPQWC ++ F PPP  +   L+D   S+   SPL+
Sbjct: 3   SDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLV 62

Query: 67  VAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRA-----INETRR---- 126
           +A+ GI A+AF+L  YY +VSKYC     +      E + E+ R+     +N   R    
Sbjct: 63  IAIFGIFATAFLLAAYYTLVSKYCANDTTN------EAASESGRSDIILDVNSPERGDQD 122

Query: 127 -----SSAGSGLDEALIKSISVYKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSH 186
                 S+ +GLD+ LIK I  +K KK +    I G+DCS+CL EF E+ESLRLLPKC+H
Sbjct: 123 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 182

Query: 187 AFHLPCIDTWLKSNSSCPLCRSNI 190
            FH+ CID WLKS+S+CPLCR+ I
Sbjct: 183 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of Carg11032 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 9.2e-28
Identity = 79/223 (35.43%), Postives = 122/223 (54.71%), Query Frame = 0

Query: 3   SIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPP-----FFGLDDSDDSSTFF 62
           S G+ DP  P+N +   D    + +   P W +   P P P        L   D SS+  
Sbjct: 24  SNGVCDPICPYNCYPEPD--YYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 83

Query: 63  SPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGG-----------PEESFETNRA 122
           +  I+ + G + +  +L+T + +V+K+   SV     G             EE F+    
Sbjct: 84  AISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ 143

Query: 123 INETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHA 182
           ++        +GL +++I SI++  +K+G+GLIE +DC VCL+EF+E+ESLRLLPKC+HA
Sbjct: 144 VDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHA 203

Query: 183 FHLPCIDTWLKSNSSCPLCRSNIASTN-PPPQETPPPPVTALG 209
           FH+ CIDTWL S+++CPLCR+ IA  +   P+ + P  VT  G
Sbjct: 204 FHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGG 242

BLAST of Carg11032 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-27
Identity = 78/184 (42.39%), Postives = 106/184 (57.61%), Query Frame = 0

Query: 25  SCTAFCP-QWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIG-ILASAFVLVTYYAIVS 84
           SC  F   ++C   +PPPPP           +   P  + V+G I+   F++   Y  ++
Sbjct: 11  SCLQFKDLRFCFRQYPPPPP---PPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHIT 70

Query: 85  KYCR---RSVGDGGGGGPEESFET-NRAINETRR--SSAGSGLDEALIKSISVYKFKKGE 144
           +  R    SV  G     E+  ET  R  ++          GL  + I SI+V  FKKGE
Sbjct: 71  QQRRISAASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGE 130

Query: 145 GLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSNI-ASTNPP 200
           G+I+G++CSVCL+EF+E+ESLRLLPKCSHAFHL CIDTWL S+ +CPLCR+ +   T PP
Sbjct: 131 GIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPP 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026103.12.4e-203100.00RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022964349.11.2e-20299.72RING-H2 finger protein ATL52-like [Cucurbita moschata][more]
KAG6593770.13.5e-20299.45RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023000254.14.8e-19697.24RING-H2 finger protein ATL52-like [Cucurbita maxima][more]
XP_023513580.13.1e-19596.69RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LF646.8e-6039.95RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ82.7e-5638.21RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY82.2e-5038.50E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0413.5e-4045.10Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q8LFY81.3e-2635.43RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HMW75.8e-20399.72RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384... [more]
A0A6J1KJE32.3e-19697.24RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 P... [more]
A0A6J1H2X89.0e-15679.23RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995... [more]
A0A6J1L4821.1e-15378.02RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 P... [more]
A0A6J1DSH11.3e-14976.86RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102276... [more]
Match NameE-valueIdentityDescription
AT5G17600.14.9e-6139.95RING/U-box superfamily protein [more]
AT3G03550.11.9e-5738.21RING/U-box superfamily protein [more]
AT4G17905.12.5e-4145.10RING/U-box superfamily protein [more]
AT1G72220.19.2e-2835.43RING/U-box superfamily protein [more]
AT5G10380.13.5e-2742.39RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 144..185
e-value: 6.2E-8
score: 42.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 143..186
e-value: 1.3E-13
score: 51.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 144..186
score: 13.079398
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 119..193
e-value: 2.9E-22
score: 79.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..291
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 314..337
NoneNo IPR availablePANTHERPTHR45676:SF108E3 UBIQUITIN-PROTEIN LIGASE OS04G0590900-LIKEcoord: 6..245
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 143..186
e-value: 6.5396E-18
score: 74.6457
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 138..192
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 6..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11032-RACarg11032-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane