Carg11026 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11026
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 3
Location	Carg_Chr08: 6026819 .. 6029053 (-)
RNA-Seq Expression	Carg11026
Synteny	Carg11026

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACGCTTTTTCTCTCCCACTCCTTCCTCCTCCTTCTCATCGGAGCCGCCACCACCACCACCTCCGCCATTTCCAGCGAGTATTGGTGCGTGGCTCGCAGCGACGTCAGCGACGAGGCGCTACAGACGGCGCTGAACTATGCCTGCGCCACCGGCGCCGATTGCACTCCTATTCTGCCGAACGGCCTGTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCATACGCCTTCAACAGCTTCTTCCAGCGCAAGGCCATGGCTCCTGGCTCCTGTAACTTCGCCGGCTCCGCCACCATCGCCCACTCCAACCCCAGTCCGTCCCCTTCCATTTCTTTTTTTTATTTTTTATGGAAATTATATTTTTTTAATTCTAACAATATATATATATATATAATAATAATGTTTATTTTGATTAATATATGGTTAAACTTTAGGTTTAATTATGTGACATTGTTTGTTTTGTGCAGGTTATGGATCTTGTGTTTATCCATCTTCTATCAGGTAATGCTAATTTTTTTTTTTTCAATTTTTTAAATGAAATATTAGGTTTTATTTATTTATTTATATTATTATAATTTATTAAAGTATGAATTAGAAAAACTACCCTTTATTTTATTCTAATTATTGGTGGAGATTTCTTCATGTTCAAGTAAATGGGTTCTCTTATCTGGAAGTATATTTTATTTTCTTTATTTTAAATTTTAAACCTTTCTTTAAAATATAATTAAAAAAATAAAATTATAATGGAGAGTTTCATTATAAAAATCTTGAACTTAAATTAAAATTTTAGTGCCCCTAAAATTTAAAATATATATAATAATTCTTTTAATTTTTATAAATTTTAATATTGTTTAAATGATAATTTAAATTATAATTTTGAGTTTAATATTTTTTTTCACATAAAAAGTAGAATCTAAAAAGAATATTAAATGGTGTCGAACATTTAATTTTTATTTAGATATAAAATTTAAAATTCAAAATTTAAAAATATATTAGATATTTAAAAAAAAACTAAATTAAAACGAATTCTTAATAATTAAATAGGGTGAAATAAATAAATAAATAAATATATATATATATATATATAATATTTTAAAAAACGAATATTTTATTTCTTGTCCGAAGTATTTTTTGTGGGCCTCATCTTAATCAGAGACGGTTGGATCAGTCATGCTGGGCCCCAGGTGGCGGTGAGATCCAACGGTTAGAATAGCATGGATTTACTTTTATAATTTTAAATTAGCTTTTTGACTAAAAAACGCTGCGTTTTGAGAATCTATAGAAGGCTGGTCAAGAATAGTATTGATTTAAATTAAACTTGTCGGAGCAAAGTCTTTTAAAATAATTAAATAGAAAATATATATATATATATATATATATATATATATATATATATATAATTAAAATTTTAGTTTAATTTTATCTTTTTTTTATCAAATCTAAAAATTAAAAAATATCTATTAATTTTTTTAAAAAAATTTAATTGGCCAAATATAAAAGTTAGACCGTTTTTAATTTTATATTTAATTAATATTTATTTTGTATTCAATCTAAGATTAAAATTTTAGAGTTTAATTAGACATTAGTTTATATTTAATAAATATTCGTTTTTTTAAATATTATTTTTTATTAAAAAAAAGAAAAATAAGAATTATAGATTGAGTGATGTTGGGTTGTCCCTAAAGAATCAATTCTTTCATATCATTTTGTCACAAGAACTTGTCCGACTTTTCTTTTTAAATTTGTCTTATTCAAAGAAAACGTTCATCGAGAATAAGAACGAACAGATGTATTGAGTTGTTCTGAAGATGATTTGTGATGATTTTCCAGTACTGCAGGAGGAGCAATCACACCGAACCTTCCAGGAAACACTAGCCCGACTATAACAACACCAACCACAACCCCCACCACGCCAAGCTTCAACAACGGTGGTACCGATGACACGAATGGACTAAACCCAGTATACAACCCGACATCTACCAACTCGAAAGCCTCTTCGAGTCTTACGACCTCTAAACTCTTGATCTTGATATTGTTGTCAATTGCTATCTCACTCGCTTCGTAAACAATATGTAGAGTAATGTCTAACATTGAATTTATTGGGAGACGGTTTACTCAATTTTGTTAGAAACTGGTGAGGCTTAAGAGATTGATCAAATGTATATTTTATTTGGACGTTATAGAGTCGGTTAGCTGTGAAGCTTCAATTTATACTTTATTAACATCGAA

mRNA sequence

ATGGCCACGCTTTTTCTCTCCCACTCCTTCCTCCTCCTTCTCATCGGAGCCGCCACCACCACCACCTCCGCCATTTCCAGCGAGTATTGGTGCGTGGCTCGCAGCGACGTCAGCGACGAGGCGCTACAGACGGCGCTGAACTATGCCTGCGCCACCGGCGCCGATTGCACTCCTATTCTGCCGAACGGCCTGTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCATACGCCTTCAACAGCTTCTTCCAGCGCAAGGCCATGGCTCCTGGCTCCTGTAACTTCGCCGGCTCCGCCACCATCGCCCACTCCAACCCCAGTTATGGATCTTGTGTTTATCCATCTTCTATCAGTACTGCAGGAGGAGCAATCACACCGAACCTTCCAGGAAACACTAGCCCGACTATAACAACACCAACCACAACCCCCACCACGCCAAGCTTCAACAACGGTGGTACCGATGACACGAATGGACTAAACCCAGTATACAACCCGACATCTACCAACTCGAAAGCCTCTTCGAGTCTTACGACCTCTAAACTCTTGATCTTGATATTGTTGTCAATTGCTATCTCACTCGCTTCGTAAACAATATGTAGAGTAATGTCTAACATTGAATTTATTGGGAGACGGTTTACTCAATTTTGTTAGAAACTGGTGAGGCTTAAGAGATTGATCAAATGTATATTTTATTTGGACGTTATAGAGTCGGTTAGCTGTGAAGCTTCAATTTATACTTTATTAACATCGAA

Coding sequence (CDS)

ATGGCCACGCTTTTTCTCTCCCACTCCTTCCTCCTCCTTCTCATCGGAGCCGCCACCACCACCACCTCCGCCATTTCCAGCGAGTATTGGTGCGTGGCTCGCAGCGACGTCAGCGACGAGGCGCTACAGACGGCGCTGAACTATGCCTGCGCCACCGGCGCCGATTGCACTCCTATTCTGCCGAACGGCCTGTGCTTCCTCCCCAACACCATCCAGGCCCACGCCTCATACGCCTTCAACAGCTTCTTCCAGCGCAAGGCCATGGCTCCTGGCTCCTGTAACTTCGCCGGCTCCGCCACCATCGCCCACTCCAACCCCAGTTATGGATCTTGTGTTTATCCATCTTCTATCAGTACTGCAGGAGGAGCAATCACACCGAACCTTCCAGGAAACACTAGCCCGACTATAACAACACCAACCACAACCCCCACCACGCCAAGCTTCAACAACGGTGGTACCGATGACACGAATGGACTAAACCCAGTATACAACCCGACATCTACCAACTCGAAAGCCTCTTCGAGTCTTACGACCTCTAAACTCTTGATCTTGATATTGTTGTCAATTGCTATCTCACTCGCTTCGTAA

Protein sequence

MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPILPNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTAGGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSKLLILILLSIAISLAS

Homology

[Close]

BLAST of Carg11026 vs. NCBI nr
Match: KAG7026109.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 8.1e-97
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL
Sbjct: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK
Sbjct: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180

Query: 181 LLILILLSIAISLAS 196
           LLILILLSIAISLAS
Sbjct: 181 LLILILLSIAISLAS 195

[Close]

BLAST of Carg11026 vs. NCBI nr
Match: XP_022964263.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 5.8e-95
Identity = 191/195 (97.95%), Postives = 193/195 (98.97%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFLSHSFLLLLIGA TTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL
Sbjct: 1   MATLFLSHSFLLLLIGATTTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITP+LPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTN+KASSSLTTSK
Sbjct: 121 GGAITPSLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNAKASSSLTTSK 180

Query: 181 LLILILLSIAISLAS 196
           LLILILLSIAISL S
Sbjct: 181 LLILILLSIAISLGS 195

[Close]

BLAST of Carg11026 vs. NCBI nr
Match: XP_023514932.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 187/195 (95.90%), Postives = 189/195 (96.92%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFL HSFLLLLIG  TTTTSA+SSEYWCVARSDVSDEALQTALNYACATGADCTPIL
Sbjct: 1   MATLFLYHSFLLLLIG-TTTTTSAVSSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITP+ PGNTSPTITTPTTT TTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK
Sbjct: 121 GGAITPSPPGNTSPTITTPTTTTTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180

Query: 181 LLILILLSIAISLAS 196
           LLILI LSIAISLAS
Sbjct: 181 LLILIWLSIAISLAS 194

[Close]

BLAST of Carg11026 vs. NCBI nr
Match: XP_022999864.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 1.4e-88
Identity = 182/194 (93.81%), Postives = 187/194 (96.39%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFLSHSFLLLLIG  TTTTSA+SSE+WCVARSDVS EALQTALNYACATGADCTPIL
Sbjct: 28  MATLFLSHSFLLLLIG--TTTTSAVSSEFWCVARSDVSYEALQTALNYACATGADCTPIL 87

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNG+CFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 88  PNGMCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 147

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITP+ PGNTSPTITTPTTT TTPSFNNGGTDDTNGLNPVYNPTSTNSKAS SLTTSK
Sbjct: 148 GGAITPSFPGNTSPTITTPTTTTTTPSFNNGGTDDTNGLNPVYNPTSTNSKASLSLTTSK 207

Query: 181 LLILILLSIAISLA 195
           L I+ILLSIAISLA
Sbjct: 208 LWIMILLSIAISLA 219

[Close]

BLAST of Carg11026 vs. NCBI nr
Match: XP_038875835.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida])

HSP 1 Score: 268.5 bits (685), Expect = 4.6e-68
Identity = 156/201 (77.61%), Postives = 164/201 (81.59%), Query Frame = 0


Query: 1   MATLFLSHSFLL--LLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTP 60
           MATL  SHSFLL   LIG     TSA+   YWCVARSD S EALQ AL+YACATGADC P
Sbjct: 23  MATLVFSHSFLLFFFLIG-----TSAVDGAYWCVARSDASYEALQAALDYACATGADCNP 82

Query: 61  ILPNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSIS 120
           IL NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSC+FAGSATIA S+PSYGSCVYPSS S
Sbjct: 83  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSTS 142

Query: 121 TAGGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTS----TNSKASS 180
            AGGAITP+ P NTSPTIT P T  TTPSFNNGGT DTNGLNPVYNPTS    TNS+ASS
Sbjct: 143 AAGGAITPSPPTNTSPTITVPAT--TTPSFNNGGTSDTNGLNPVYNPTSTSSPTNSRASS 202

Query: 181 SLTTSKLLILILLSIAISLAS 196
           S T  KLL+L+L SIAISL S
Sbjct: 203 SFTVPKLLVLVLFSIAISLPS 216

[Close]

BLAST of Carg11026 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.0e-25
Identity = 68/153 (44.44%), Postives = 90/153 (58.82%), Query Frame = 0


Query: 26  SSEYWCVARSDVSDEALQTALNYACATGADCTPILPNGLCFLPNTIQAHASYAFNSFFQR 85
           SS  WCV +  +S+  LQ  L+YAC  GADC PI   G CF PNT+++H SYA NSFFQ+
Sbjct: 17  SSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQK 76

Query: 86  KAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTAGGAITPNLPGNTSPTITTPTTTPTT 145
           K  + G+C+FAG+AT + S+PSY +C +P+S S +G       P  T+P+   PTTT T 
Sbjct: 77  KGQSLGTCDFAGTATFSASDPSYTTCPFPASASGSG----TTTPVTTTPSTRVPTTTNTR 136

Query: 146 P----SFNNGGTDDTNGLNPVYNPTSTNSKASS 175
           P        GG    +G+NP Y   S   K  S
Sbjct: 137 PYTITPSTGGGLGIPSGINPDYTDPSFGFKLQS 165

[Close]

BLAST of Carg11026 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 8.6e-25
Identity = 84/202 (41.58%), Postives = 112/202 (55.45%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MA L LS   LLLL      + +  SS  WCV ++ +SD  LQ  L+YAC  GADC P  
Sbjct: 1   MAALVLS---LLLL------SLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTK 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           P   CF P+ +++H +YA NSFFQ+K  +PGSCNF G+AT  +S+PSY  C +P+S S +
Sbjct: 61  PKQSCFNPDNVRSHCNYAVNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGS 120

Query: 121 GGAITPNLPGNTSPTITTPTTT-------------PTTPSFNNGGTDDT--NGLNPVYNP 180
            G+ T   PG T+P  +  TTT             PT   F    T  T   G+NP Y  
Sbjct: 121 SGSTTVT-PGTTNPKGSPTTTTLPGSGTNSPYSGNPTNGVFGGNSTGGTTGTGINPDY-- 180

Query: 181 TSTNSKASSSLTTSKLLILILL 188
            +T+S A +   +SKL I +LL
Sbjct: 181 -TTDSSAFALKNSSKLFICLLL 189

[Close]

BLAST of Carg11026 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.2e-22
Identity = 84/206 (40.78%), Postives = 112/206 (54.37%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MA L L   +LL L+ A  T+ S      WCV ++ +SD  LQ  L+YAC  GADC P  
Sbjct: 1   MAPLVL---YLLTLLMAGHTSAS------WCVCKTGLSDSVLQKTLDYACGNGADCNPTH 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSIS-T 120
           P G CF P+ ++AH +YA NSFFQ+K  A  SCNF G+AT+  ++PSY  C +PSS S +
Sbjct: 61  PKGSCFNPDNVRAHCNYAVNSFFQKKGQASESCNFTGTATLTTTDPSYTGCAFPSSASGS 120

Query: 121 AGGAITPNLPGNTSP----TITT-------PTTTPTTPSFNNGGTDDT-NGLNPVYNPTS 180
           +G   T   PG  SP    +ITT        + TP+T       TD T  GLNP Y   S
Sbjct: 121 SGSGSTTVTPGKNSPKGSNSITTFPGGNSPYSGTPSTGLLGGNITDATGTGLNPDY---S 180

Query: 181 TNSKASSSLTTSKLLILILLSIAISL 194
           T S   +   ++ LL+    S+ + L
Sbjct: 181 TESSGFALYYSNNLLLTGFCSLVMML 194

[Close]

BLAST of Carg11026 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 44/98 (44.90%), Postives = 64/98 (65.31%), Query Frame = 0


Query: 18  ATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPILPNGLCFLPNTIQAHASY 77
           AT T +  ++  WCV +  VSD+ L   +NYAC  G DC PI P G CF PNT++AHA+Y
Sbjct: 360 ATPTPTPKAAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAY 419

Query: 78  AFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPS 116
             N ++Q       +C+F+ +AT+ ++NPSYG+C +PS
Sbjct: 420 VMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

[Close]

BLAST of Carg11026 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 43/108 (39.81%), Postives = 72/108 (66.67%), Query Frame = 0


Query: 10  FLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATG-ADCTPILPNGLCFLP 69
           +LL + G+ T   +  +++ +C+A   V  + LQ AL++AC  G ++C+ I P   C+ P
Sbjct: 361 YLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQP 420

Query: 70  NTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSS 117
           N ++ HAS+AFNS++Q++  A GSC+F G A I  ++PS+GSC++P S
Sbjct: 421 NNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIFPGS 468

[Close]

BLAST of Carg11026 vs. ExPASy TrEMBL
Match: A0A6J1HKA9 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LOC111464333 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.8e-95
Identity = 191/195 (97.95%), Postives = 193/195 (98.97%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFLSHSFLLLLIGA TTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL
Sbjct: 1   MATLFLSHSFLLLLIGATTTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITP+LPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTN+KASSSLTTSK
Sbjct: 121 GGAITPSLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNAKASSSLTTSK 180

Query: 181 LLILILLSIAISLAS 196
           LLILILLSIAISL S
Sbjct: 181 LLILILLSIAISLGS 195

[Close]

BLAST of Carg11026 vs. ExPASy TrEMBL
Match: A0A6J1KIB1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494211 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.7e-89
Identity = 182/194 (93.81%), Postives = 187/194 (96.39%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATLFLSHSFLLLLIG  TTTTSA+SSE+WCVARSDVS EALQTALNYACATGADCTPIL
Sbjct: 28  MATLFLSHSFLLLLIG--TTTTSAVSSEFWCVARSDVSYEALQTALNYACATGADCTPIL 87

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNG+CFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA
Sbjct: 88  PNGMCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 147

Query: 121 GGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTSK 180
           GGAITP+ PGNTSPTITTPTTT TTPSFNNGGTDDTNGLNPVYNPTSTNSKAS SLTTSK
Sbjct: 148 GGAITPSFPGNTSPTITTPTTTTTTPSFNNGGTDDTNGLNPVYNPTSTNSKASLSLTTSK 207

Query: 181 LLILILLSIAISLA 195
           L I+ILLSIAISLA
Sbjct: 208 LWIMILLSIAISLA 219

[Close]

BLAST of Carg11026 vs. ExPASy TrEMBL
Match: A0A1S3C4M3 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.0e-64
Identity = 147/199 (73.87%), Postives = 160/199 (80.40%), Query Frame = 0


Query: 1   MATLFLSH--SFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTP 60
           MAT   SH  SF L         TSA+   YWCVARSD + E+LQ A++YACATGADCTP
Sbjct: 1   MATFLPSHYYSFFLFFFLFFLIGTSAVDGAYWCVARSDATYESLQAAIDYACATGADCTP 60

Query: 61  ILPNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSIS 120
           IL NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSC+F GSATIA S+PSYGSCVYPSS+S
Sbjct: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSYGSCVYPSSLS 120

Query: 121 TAGGAITPNLPGNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTS----TNSKASS 180
           +AGGAITP+ P NTSPTIT P T   TPSFNNGGT DTNG+NPVYNPTS    TNS+ASS
Sbjct: 121 SAGGAITPSPPANTSPTITVPGT--ATPSFNNGGTGDTNGVNPVYNPTSTSNPTNSEASS 180

Query: 181 SLTTSKLLILILLSIAISL 194
           S TT KL+ILIL SIAISL
Sbjct: 181 SFTTPKLVILILFSIAISL 197

[Close]

BLAST of Carg11026 vs. ExPASy TrEMBL
Match: A0A6J1H812 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460407 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.7e-64
Identity = 152/201 (75.62%), Postives = 162/201 (80.60%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATL LSHSFLLLL+      TSA+   YWCVARS+ S EALQTAL+YACATGADCTP+L
Sbjct: 1   MATLVLSHSFLLLLL-LFLLPTSAVDGAYWCVARSEASYEALQTALDYACATGADCTPLL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
            NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSC+FAGSATIA ++PSYGSCVYPSSIS A
Sbjct: 61  SNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQTDPSYGSCVYPSSISAA 120

Query: 121 GGAITPNLPGNTS--PTITTPTTTPTTPSFNNGGTDDTNGLNPVYNP----TSTNSKASS 180
           GGAITP+ P NTS  PTIT P TT TTPSFNNG T D NGL+PVYNP    T TNSKASS
Sbjct: 121 GGAITPSPPANTSPTPTITVPATT-TTPSFNNGNTGDMNGLDPVYNPASTSTPTNSKASS 180

Query: 181 SLTTSKLLILILLSIAISLAS 196
           S    KL    L SIAISLAS
Sbjct: 181 SFRIPKL----LFSIAISLAS 195

[Close]

BLAST of Carg11026 vs. ExPASy TrEMBL
Match: A0A6J1KVB1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499021 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.1e-62
Identity = 142/197 (72.08%), Postives = 160/197 (81.22%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MATL LSHSFLLLL+      TSA+   YWCVARS+ S EALQTAL+YACATGADCTP+L
Sbjct: 1   MATLVLSHSFLLLLL-LFLLPTSAVDGAYWCVARSEASYEALQTALDYACATGADCTPLL 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
            NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSC+FAGSAT+A ++PSYGSCVYPSSIS A
Sbjct: 61  SNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATVAQTDPSYGSCVYPSSISAA 120

Query: 121 GGAITPNLPGNTS--PTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTT 180
           GGAITP+ P NTS  PTIT P TT TTPSFNNG + D NGL+PVYNP ST++  +S  ++
Sbjct: 121 GGAITPSPPANTSPTPTITVPATT-TTPSFNNGNSGDINGLDPVYNPASTSTPTNSKASS 180

Query: 181 SKLLILILLSIAISLAS 196
           S  +   L SIAISL S
Sbjct: 181 SFRIPKFLFSIAISLTS 195

[Close]

BLAST of Carg11026 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-41
Identity = 106/201 (52.74%), Postives = 131/201 (65.17%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MA    + SFLLLL+  +       S+  WCVAR DV+ +ALQ AL+YACA GADC PI 
Sbjct: 39  MALCLQTLSFLLLLLFRS-------SAAMWCVARFDVTSQALQAALDYACAAGADCAPIQ 98

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           PNGLCFLPNT+QAHASYAFNS+FQR AMAPGSCNFAG++TIA ++PSYGSCVYP+S+  A
Sbjct: 99  PNGLCFLPNTVQAHASYAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYPNSVRNA 158

Query: 121 GGAI-------TPNLP-GNTSPTITTPTTTPTTPSFNNGGTDDTNGLNPVYNPTSTNSKA 180
           GG+        TP+   GN+  T   P +  TT  F  GG     GLNP    T+TN+  
Sbjct: 159 GGSASTTTVGGTPSTTVGNSPMTTLRPPSGTTTSPFGIGG----GGLNPQGTTTTTNTDE 218

Query: 181 SSSLTTSKLLILILLSIAISL 194
           S S   +K  + I L +A+ L
Sbjct: 219 SGSYIINKSTVSIALFVALLL 228

[Close]

BLAST of Carg11026 vs. TAIR 10
Match: AT1G18650.1 (plasmodesmata callose-binding protein 3 )

HSP 1 Score: 115.9 bits (289), Expect = 3.6e-26
Identity = 68/153 (44.44%), Postives = 90/153 (58.82%), Query Frame = 0


Query: 26  SSEYWCVARSDVSDEALQTALNYACATGADCTPILPNGLCFLPNTIQAHASYAFNSFFQR 85
           SS  WCV +  +S+  LQ  L+YAC  GADC PI   G CF PNT+++H SYA NSFFQ+
Sbjct: 17  SSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQK 76

Query: 86  KAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTAGGAITPNLPGNTSPTITTPTTTPTT 145
           K  + G+C+FAG+AT + S+PSY +C +P+S S +G       P  T+P+   PTTT T 
Sbjct: 77  KGQSLGTCDFAGTATFSASDPSYTTCPFPASASGSG----TTTPVTTTPSTRVPTTTNTR 136

Query: 146 P----SFNNGGTDDTNGLNPVYNPTSTNSKASS 175
           P        GG    +G+NP Y   S   K  S
Sbjct: 137 PYTITPSTGGGLGIPSGINPDYTDPSFGFKLQS 165

[Close]

BLAST of Carg11026 vs. TAIR 10
Match: AT5G61130.1 (plasmodesmata callose-binding protein 1 )

HSP 1 Score: 115.2 bits (287), Expect = 6.1e-26
Identity = 84/202 (41.58%), Postives = 112/202 (55.45%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MA L LS   LLLL      + +  SS  WCV ++ +SD  LQ  L+YAC  GADC P  
Sbjct: 1   MAALVLS---LLLL------SLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTK 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSISTA 120
           P   CF P+ +++H +YA NSFFQ+K  +PGSCNF G+AT  +S+PSY  C +P+S S +
Sbjct: 61  PKQSCFNPDNVRSHCNYAVNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGS 120

Query: 121 GGAITPNLPGNTSPTITTPTTT-------------PTTPSFNNGGTDDT--NGLNPVYNP 180
            G+ T   PG T+P  +  TTT             PT   F    T  T   G+NP Y  
Sbjct: 121 SGSTTVT-PGTTNPKGSPTTTTLPGSGTNSPYSGNPTNGVFGGNSTGGTTGTGINPDY-- 180

Query: 181 TSTNSKASSSLTTSKLLILILL 188
            +T+S A +   +SKL I +LL
Sbjct: 181 -TTDSSAFALKNSSKLFICLLL 189

[Close]

BLAST of Carg11026 vs. TAIR 10
Match: AT5G08000.1 (glucan endo-1,3-beta-glucosidase-like protein 3 )

HSP 1 Score: 105.9 bits (263), Expect = 3.7e-23
Identity = 84/206 (40.78%), Postives = 112/206 (54.37%), Query Frame = 0


Query: 1   MATLFLSHSFLLLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPIL 60
           MA L L   +LL L+ A  T+ S      WCV ++ +SD  LQ  L+YAC  GADC P  
Sbjct: 1   MAPLVL---YLLTLLMAGHTSAS------WCVCKTGLSDSVLQKTLDYACGNGADCNPTH 60

Query: 61  PNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPSYGSCVYPSSIS-T 120
           P G CF P+ ++AH +YA NSFFQ+K  A  SCNF G+AT+  ++PSY  C +PSS S +
Sbjct: 61  PKGSCFNPDNVRAHCNYAVNSFFQKKGQASESCNFTGTATLTTTDPSYTGCAFPSSASGS 120

Query: 121 AGGAITPNLPGNTSP----TITT-------PTTTPTTPSFNNGGTDDT-NGLNPVYNPTS 180
           +G   T   PG  SP    +ITT        + TP+T       TD T  GLNP Y   S
Sbjct: 121 SGSGSTTVTPGKNSPKGSNSITTFPGGNSPYSGTPSTGLLGGNITDATGTGLNPDY---S 180

Query: 181 TNSKASSSLTTSKLLILILLSIAISL 194
           T S   +   ++ LL+    S+ + L
Sbjct: 181 TESSGFALYYSNNLLLTGFCSLVMML 194

[Close]

BLAST of Carg11026 vs. TAIR 10
Match: AT1G13830.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 75/176 (42.61%), Postives = 102/176 (57.95%), Query Frame = 0


Query: 12  LLLIGAATTTTSAISSEYWCVARSDVSDEALQTALNYACATGADCTPILPNGLCFLPNTI 71
           LLL+ + T ++SAI    +C+ +  + D  LQT+++YAC T ADC PI   G C+ P+TI
Sbjct: 7   LLLLLSLTNSSSAI----YCLCKDGIGDTELQTSIDYACGTLADCNPIHDKGTCYQPDTI 66

Query: 72  QAHASYAFNSFFQRKAMAPGSCNFAGSATIAHSNPS--YGSCVYPSSISTAGGAITPNLP 131
           ++H  +A NS+FQ  A  PGSCNF+G+AT   + PS     C+YPSS S+     T + P
Sbjct: 67  KSHCDWAVNSYFQNAAQVPGSCNFSGTATTNPNPPSNLANGCIYPSSPSS-----TRSPP 126

Query: 132 GNTSPTITTPT--TTP----TTPSFNNGGTDDTNGLNPVYNPTSTNSKASSSLTTS 180
             T PT TTPT  TTP      P     GT    G   V+NP++  S ASS  T+S
Sbjct: 127 STTPPTGTTPTNGTTPFPGTPFPGTPFPGTPPVFGPTGVFNPSNPGSGASSLGTSS 173

The following BLAST results are available for this feature:

BLAST of Carg11026 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026109.1	8.1e-97	100.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022964263.1	5.8e-95	97.95	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like [Cucurbita moschata]	[more]
XP_023514932.1	1.1e-90	95.90	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like isoform X1 [Cucurbita pepo subsp. p...	[more]
XP_022999864.1	1.4e-88	93.81	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Cucurbita maxima]	[more]
XP_038875835.1	4.6e-68	77.61	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida]	[more]

BLAST of Carg11026 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ86	5.0e-25	44.44	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3...	[more]
Q9FNQ2	8.6e-25	41.58	PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1...	[more]
Q9SD84	5.2e-22	40.78	PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2...	[more]
Q94G86	1.4e-19	44.90	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
O65399	1.3e-17	39.81	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]

BLAST of Carg11026 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HKA9	2.8e-95	97.95	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1KIB1	6.7e-89	93.81	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A1S3C4M3	2.0e-64	73.87	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 ...	[more]
A0A6J1H812	5.7e-64	75.62	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1KVB1	4.1e-62	72.08	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499...	[more]

BLAST of Carg11026 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13600.1	1.3e-41	52.74	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G18650.1	3.6e-26	44.44	plasmodesmata callose-binding protein 3	[more]
AT5G61130.1	6.1e-26	41.58	plasmodesmata callose-binding protein 1	[more]
AT5G08000.1	3.7e-23	40.78	glucan endo-1,3-beta-glucosidase-like protein 3	[more]
AT1G13830.1	2.4e-22	42.61	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 29..113 e-value: 2.3E-44 score: 163.4
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 30..100 e-value: 1.3E-20 score: 73.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 22..120 e-value: 1.6E-10 score: 43.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 128..173
None	No IPR available	PANTHER	PTHR31044:SF47	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 10..183
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 10..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11026-RA	Carg11026-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma