Carg11014 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11014
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectate lyase
LocationCarg_Chr08: 6115148 .. 6119577 (+)
RNA-Seq ExpressionCarg11014
SyntenyCarg11014
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTATCGTCTTTCCGTTGGAGGTATGTCTATAAAAGGTCCGTTTTGTCCAAAGCTTTCACTGACATCACCACTCGTTTCCTCTGTTCTTCTTCTTCTTCTTCTTCTTCCTTTTCTCTCCACATTTCTCCTTCTCCATAATCTCTCTCTCTTAATTCCCTCTGGTTTTTCTCAAGAGCGTGAACGAGAGAGAGAGAGAGAGGTCAGTAATGGCGGCACTCAGCAAATGGTCTTCTCTGTCTCTGCTTGCGCTTGCAATGGTGTTCTTCGTAACAATGGCTCTGGTGCGCAAAGAAGAGGTCTCTGGAATTAGGTAAATCTTTTCTCCCTTTCATATTCTTCTCTTACAATCTCAATGTGATGTGTTTGATTTGATAATTCTAGCCACATTGTGTGAATTTCTTGTTGGGAGAAAATGCTAGGTTTGTATAGTACTGATTCTTTTCGGTTTCAATTTGGAGACTCTTGAATGTTCAATGTGGATGTATGATTCAATATTATTTAGTGTTTTTTCTTAGAATATTGGATCAATGCTGTAATGTCGAAATGAGATAAAGTAAAACTACGCTCCACTGCTTTAATGGCGGACTTCAGTTCAAAATTCCTTTTACTAAAGGAGTGAGGAATAAAAACAGAATCTCTTAATTTCCAACGAACTGTAGCGGTTTTTGAATTGATTTTTGTTCTTTTTTCATTTTACAGGACTGTGTTAGCGACGAAGTTTCAGAGCTCTATCAACTCATCAATGGCGACTAGGTTAGTTTTCGTCTCATTAACTCGAGTAATGAAAGGAAGGGGAAAATCTGTACCGTAATGAAATGATCTCAACCGTACGTTCTGCCCATCTTGCTAATCACAGCCGTTGGATTCTCCTCGGATCCGTGTGATGTGATGACCCAACTGTAGCTAACGATCTCAGCCGTTGAGATCCAGATCTTCAATCTGGACCGTTTGTTCCGTTTTCAGTTACTCCCCCTTAAGATTCCTTCCCGTAATTACCCCCACAGGCTCCCTGTCATTTCCCTCGCGTTTCAGTCATTTCATCAACAAGAATAGAAGGACGGTTTCTTTCTCCTTTTATTTTCTTATTAATTCTTTGGGTGTTCCGTTTTTCCTTTTTCCTTCCCTCTAGTGCCGACCAATCGTCTTGCTACAATTCCCTCTCTCTAAACAAAAGCGCTTTTCCCAACGGTTTTCTCTTTGTGACAGAACGACAGTACGGCGGGCTGCGTGAAAGATCTCTTCCGCACCCACCTTGAGCGTTGAAGCCACTCACTAACTTCTTCATTTCCATTCCTCACGCGCCCTCTGATATTCCACGCGCCCTTTTCTAATCTGGCTCTACTTTGAAGTTTAAATCTCCTCTAATATGGCTAATTGTTAAGTGAGAATAAAAAGATAATATCTAGGTGGGTTTGTCACTTAATTGGGTGATTCTGTTTACAGGGCGGTGAATGATGATTCGGAGGAACTTAACAATGAGCATGCTGTGGATAATCCTGACGAGATTGCTGCCTCTGTTGAGATGTGAGTCTTTTTCTTTCATTCATTCTATATTTGAATCATTGATTCTTTTGTTATCGTTAAGATGTCTATATCTTGTTAAGAAATTCATTATTAGAGAGATTGGTTTTGTGAAATTTGGATCGAATTGGATTTTTCTCTTGTAATTAGTAATGGGTCTTGTAGTAATTAAATACCGAGCTGCACATCGCACATGCGGTTGTTTGTTTCGTTTTTGTCTGTATCGTAAAATCTTAGGAAGAGAATTTATAGACGTTACAATGAAAGTCTTCGTGGTCAAAATGGCAGCATGATTTTGTCATTTTCTGTTTTAGTGATATGCGTTCTTTTATTACCGTTGTGATTGAAAACGTGCATTACTGGCGCCCCTGACATTCGCTTTCCGACAAATTCTTGATTGTTCTCGATATTTGTACATGGAGATTGTGATGGGGTTTCCCATAACTTTTATCCTATCTTTGGTTTTTCATTGGACGTGCAATTTCAGAACTCGAGAAATTTGTCCTAAGAATGTTCTTTGATATGGCAGGAGCATCCGTAACAGCACTGAAAGGAGAAAATTGGGATTTTTCTCCTGTGGAACTGGCAATCCCATTGATGACTGTTGGCGTTGCGACCCCAATTGGCACCACAAACGTAAACGCCTAGCTGAATGTGGCATTGGGTTCGGCCGAAATGCTATCGGTGGTCGTGATGGTCGTTTCTACGTTGTCACTGATTCGAGCGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTCATTCAAGAGAAACCTCTTTGGATCGTATTCAAAAGGGACATGGTGATTCAATTGAAACAAGAACTCATCATGAACAGTTTTAAGACCATTGATGCTCGCGGGGTCAATGTACACATTGCCAATGGCGCTTGCATCACAATACAGTTTGTGACTAATATCATTATCCATGGTCTTCACATCCATGATTGCAAGCCCACAGGAAATGCCATGGTCAGAAGCTCTCCAACTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGGTCAAGCCATATATGGATCGACCACAATTCACTCTCTAATTGTGCCGATGGCCTTGTTGATGCTGTCATGGGCTCAACTGCAATCACCATCTCCAACAATCACTTCACCCACCACAATGAGGTTCACTTTCTATTGCATTTCTGATATCAAATCAAGTTAGAAGTAACAAATTTATCTGATGAAATTTGATCTTAAATGGTAACAGGTGATGCTTCTAGGCCATAGTGATTCCTACACAAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAGGGACTCATTCAAAGAATGCCAAGGTAAGCTCGTCTTTTATACGTGAAGAAGATGGTTTAAATTGTGTTGAGTTTGTCAACAGCGATTTCTCACCCCACATAAATTTCAATGATGTGTATAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCATTGGGAGATGTATGCCATTGGTGGGAGTGCAAACCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCCTTTTGCCAAAGAGGTAATTAACTCCCCCAAAAAATCATTTCAACTTAGATTTAGACACTTGATGAGGCGGGCTTGTAACCGCCTAAGCCCACCACCAGTAGGTATTGTCCTCTTTGTGTTTTTCCCTTCGAACTTTCCCTAAAGGTTTACCGCTAGCAGATATTGTCTTCTTTGGACTTTCTGCTTCGAACTTCCCCTCAAAGTTTTTAAAACGCTTTTGTTAGGGAGAATTTTCACACCTTTATAAGTAATGTTTCTTACTCCTTCCCAACCGACGTGGGATCTCACAATCCACTCTCCTTTAAGGCTCGGCATCTCGCTAGCACTCGTTCCTTTTTCTAATCAATGTGGGACCACAATTTCACCCCCTTTCGGGGCCCAACGTCCTTGGCACACCGCTCGTGTCCACCCTCAATCATTGTGGGACCTACCAATCTACCCCTTCGAGGCCCAGTGTCCTTACTTGCACACCGCCTCGTGTCCACCCTTTCAGAGCTCAGCTTCTTTGTTGGCACATCGCCCGATTCTGATACTATTTGTAACCGTTCAAGCCTACTCTTAGCAAATATTATACTTTTTGGGCTTTTCCGTTCGGGCTTCTTCTCAAAATTTTTAAAATAGGTTTACAATGGAAACCTCACCTTATAAAAAATGTTTTGTTCTCCATTCCAATCGAGGGATCTCACTGGGTTATGCAGGTGACCAAACGAGTTGAAACAGCAGAGAGCGAATGGAAAGGGTGGAACTGGAGATCAGAAGGAGATATGCTGCTGAACGGTGCTTATTTCACCCCATCAGGCGCTGGAGCTTCAGCAAGCTATGCGAGAGCCTCCAGCTTAGGAGCCAAATCCGCTTCCATGGTTGCCTCCATCACTTCTTCAGCAGGTTCGCTCCCTTGCCGCCGTGGCCATCCCTGCTAGTCCTGTCCCTATCATCTTAACACCAAAAAAAAAAGAAAAAAAAAAGAAAACTTCATGATTTCTCATCCATTCCCATTGCCCCCTTCTTTATAGCCATTTAACAAACACATTTTTTCCCCCTTCAAAACTTGTTTTAAAACCAAACCCCTTTGTTGTTCATCTGTTGGTTGCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTAAGGGGCAGGTGCAGAAGCGTTGTCCTTCTTCAAATTTTCTTTTCCTCAATTTGTCTGGTTTTTTACCATATGCTTCGAGTCATTTTGTGATGCAATATTGTCGGTCATTACACTATGTTTTCACTCCTGGTTTTGTAATTTTTTTTTTAACCTTTTGAGTTCATGACAGAAACAGCTTAAAGAAGATTACA

mRNA sequence

GTTATCGTCTTTCCGTTGGAGGTATGTCTATAAAAGGTCCGTTTTGTCCAAAGCTTTCACTGACATCACCACTCGTTTCCTCTGTTCTTCTTCTTCTTCTTCTTCTTCCTTTTCTCTCCACATTTCTCCTTCTCCATAATCTCTCTCTCTTAATTCCCTCTGGTTTTTCTCAAGAGCGTGAACGAGAGAGAGAGAGAGAGGTCAGTAATGGCGGCACTCAGCAAATGGTCTTCTCTGTCTCTGCTTGCGCTTGCAATGGTGTTCTTCGTAACAATGGCTCTGGTGCGCAAAGAAGAGGTCTCTGGAATTAGGACTGTGTTAGCGACGAAGTTTCAGAGCTCTATCAACTCATCAATGGCGACTAGGGCGGTGAATGATGATTCGGAGGAACTTAACAATGAGCATGCTGTGGATAATCCTGACGAGATTGCTGCCTCTGTTGAGATGAGCATCCGTAACAGCACTGAAAGGAGAAAATTGGGATTTTTCTCCTGTGGAACTGGCAATCCCATTGATGACTGTTGGCGTTGCGACCCCAATTGGCACCACAAACGTAAACGCCTAGCTGAATGTGGCATTGGGTTCGGCCGAAATGCTATCGGTGGTCGTGATGGTCGTTTCTACGTTGTCACTGATTCGAGCGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTCATTCAAGAGAAACCTCTTTGGATCGTATTCAAAAGGGACATGGTGATTCAATTGAAACAAGAACTCATCATGAACAGTTTTAAGACCATTGATGCTCGCGGGGTCAATGTACACATTGCCAATGGCGCTTGCATCACAATACAGTTTGTGACTAATATCATTATCCATGGTCTTCACATCCATGATTGCAAGCCCACAGGAAATGCCATGGTCAGAAGCTCTCCAACTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGGTCAAGCCATATATGGATCGACCACAATTCACTCTCTAATTGTGCCGATGGCCTTGTTGATGCTGTCATGGGCTCAACTGCAATCACCATCTCCAACAATCACTTCACCCACCACAATGAGGTGATGCTTCTAGGCCATAGTGATTCCTACACAAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAGGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCATTGGGAGATGTATGCCATTGGTGGGAGTGCAAACCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCCTTTTGCCAAAGAGGTGACCAAACGAGTTGAAACAGCAGAGAGCGAATGGAAAGGGTGGAACTGGAGATCAGAAGGAGATATGCTGCTGAACGGTGCTTATTTCACCCCATCAGGCGCTGGAGCTTCAGCAAGCTATGCGAGAGCCTCCAGCTTAGGAGCCAAATCCGCTTCCATGGTTGCCTCCATCACTTCTTCAGCAGGTTCGCTCCCTTGCCGCCGTGGCCATCCCTGCTAGTCCTGTCCCTATCATCTTAACACCAAAAAAAAAAGAAAAAAAAAAGAAAACTTCATGATTTCTCATCCATTCCCATTGCCCCCTTCTTTATAGCCATTTAACAAACACATTTTTTCCCCCTTCAAAACTTGTTTTAAAACCAAACCCCTTTGTTGTTCATCTGTTGGTTGCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTAAGGGGCAGGTGCAGAAGCGTTGTCCTTCTTCAAATTTTCTTTTCCTCAATTTGTCTGGTTTTTTACCATATGCTTCGAGTCATTTTGTGATGCAATATTGTCGGTCATTACACTATGTTTTCACTCCTGGTTTTGTAATTTTTTTTTTAACCTTTTGAGTTCATGACAGAAACAGCTTAAAGAAGATTACA

Coding sequence (CDS)

ATGGCGGCACTCAGCAAATGGTCTTCTCTGTCTCTGCTTGCGCTTGCAATGGTGTTCTTCGTAACAATGGCTCTGGTGCGCAAAGAAGAGGTCTCTGGAATTAGGACTGTGTTAGCGACGAAGTTTCAGAGCTCTATCAACTCATCAATGGCGACTAGGGCGGTGAATGATGATTCGGAGGAACTTAACAATGAGCATGCTGTGGATAATCCTGACGAGATTGCTGCCTCTGTTGAGATGAGCATCCGTAACAGCACTGAAAGGAGAAAATTGGGATTTTTCTCCTGTGGAACTGGCAATCCCATTGATGACTGTTGGCGTTGCGACCCCAATTGGCACCACAAACGTAAACGCCTAGCTGAATGTGGCATTGGGTTCGGCCGAAATGCTATCGGTGGTCGTGATGGTCGTTTCTACGTTGTCACTGATTCGAGCGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTCATTCAAGAGAAACCTCTTTGGATCGTATTCAAAAGGGACATGGTGATTCAATTGAAACAAGAACTCATCATGAACAGTTTTAAGACCATTGATGCTCGCGGGGTCAATGTACACATTGCCAATGGCGCTTGCATCACAATACAGTTTGTGACTAATATCATTATCCATGGTCTTCACATCCATGATTGCAAGCCCACAGGAAATGCCATGGTCAGAAGCTCTCCAACTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGGTCAAGCCATATATGGATCGACCACAATTCACTCTCTAATTGTGCCGATGGCCTTGTTGATGCTGTCATGGGCTCAACTGCAATCACCATCTCCAACAATCACTTCACCCACCACAATGAGGTGATGCTTCTAGGCCATAGTGATTCCTACACAAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAGGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCATTGGGAGATGTATGCCATTGGTGGGAGTGCAAACCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCCTTTTGCCAAAGAGGTGACCAAACGAGTTGAAACAGCAGAGAGCGAATGGAAAGGGTGGAACTGGAGATCAGAAGGAGATATGCTGCTGAACGGTGCTTATTTCACCCCATCAGGCGCTGGAGCTTCAGCAAGCTATGCGAGAGCCTCCAGCTTAGGAGCCAAATCCGCTTCCATGGTTGCCTCCATCACTTCTTCAGCAGGTTCGCTCCCTTGCCGCCGTGGCCATCCCTGCTAG

Protein sequence

MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSEELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC
Homology
BLAST of Carg11014 vs. NCBI nr
Match: KAG6593793.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026121.1 putative pectate lyase 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 909.4 bits (2349), Expect = 1.2e-260
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. NCBI nr
Match: XP_022964161.1 (probable pectate lyase 8 [Cucurbita moschata] >XP_022964162.1 probable pectate lyase 8 [Cucurbita moschata] >XP_022964163.1 probable pectate lyase 8 [Cucurbita moschata])

HSP 1 Score: 908.3 bits (2346), Expect = 2.6e-260
Identity = 447/448 (99.78%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRK+EVSGIRTVLATKFQSSINSSMATRAVNDDSE
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKQEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. NCBI nr
Match: XP_023515191.1 (probable pectate lyase 8 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 905.2 bits (2338), Expect = 2.2e-259
Identity = 446/448 (99.55%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRKEEVS IRTVLATKFQSSINSSMATRAVNDDSE
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSEIRTVLATKFQSSINSSMATRAVNDDSE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAE+EWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAENEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. NCBI nr
Match: XP_023000287.1 (probable pectate lyase 8 [Cucurbita maxima])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 446/448 (99.55%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRKEEVS IRTVLATKFQSSINSSMATRAVNDD E
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSEIRTVLATKFQSSINSSMATRAVNDDLE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. NCBI nr
Match: XP_022138849.1 (probable pectate lyase 8 [Momordica charantia])

HSP 1 Score: 870.5 bits (2248), Expect = 6.1e-249
Identity = 428/448 (95.54%), Postives = 434/448 (96.88%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALS+WSSLSL+ALAMVFFVTMALVR+E+ S IRT LA +FQSS NSSMA R  NDD E
Sbjct: 1   MAALSRWSSLSLVALAMVFFVTMALVRREQTSEIRTELAAEFQSSSNSSMAARVENDDVE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           +L NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD NWH  RKRLA
Sbjct: 61  KLKNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMV SITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVGSITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 704.9 bits (1818), Expect = 5.8e-202
Identity = 335/410 (81.71%), Postives = 368/410 (89.76%), Query Frame = 0

Query: 43  QSSINSSMATRAVNDDSEELNNEHAVDNPDEIA----ASVEMSIRNSTERRKLGFFSCGT 102
           +++ + ++++   +D++E   N+HAV NPDE+A    A  EMS+RN TERRKLG+F+CGT
Sbjct: 22  ENTTHDNISSLPRSDETEW--NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGT 81

Query: 103 GNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLR 162
           GNPIDDCWRCDPNWH  RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLR
Sbjct: 82  GNPIDDCWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLR 141

Query: 163 HAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHG 222
           HAVIQ++PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HG
Sbjct: 142 HAVIQDRPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHG 201

Query: 223 LHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 282
           LHIHDCKPTGNAMVRSS THFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMG
Sbjct: 202 LHIHDCKPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMG 261

Query: 283 STAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 342
           STAITISNNH THHNEVMLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVV
Sbjct: 262 STAITISNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVV 321

Query: 343 NNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDM 402
           NNDYTHWEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  S WKGWNWRSEGD+
Sbjct: 322 NNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDL 381

Query: 403 LLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           L NGAYFT SGA AS SYARASSL AKS+S+V  ITS AG+LPCRRG  C
Sbjct: 382 LQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Carg11014 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 8.4e-201
Identity = 352/470 (74.89%), Postives = 388/470 (82.55%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMAT-------- 60
           MA+ S+      +A+ +V  +T  + R  E+S  R +     QSS +S+MA         
Sbjct: 1   MASSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEE 60

Query: 61  ---------RAVNDDSEELNNEHAV-DNP----DEIAASVEMSIRNSTERRKLGFFSCGT 120
                     A   D E    +HAV D+P    DE+A  V+MS +N T RRKLGFFSCGT
Sbjct: 61  FQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLR 180
           GNPIDDCWRCD NWH  RKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLR
Sbjct: 121 GNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLR 180

Query: 181 HAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHG 240
           HAVIQE+PLWIVFKRDMVI+LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHG
Sbjct: 181 HAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LHIHDCKPTGNAMVRSSP+HFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMG 300

Query: 301 STAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAIT+SNNHFTHHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVV
Sbjct: 301 STAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDM 420
           NNDYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  SEWK WNWRSEGD+
Sbjct: 361 NNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDL 420

Query: 421 LLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           LLNGA+F PSGAGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 421 LLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of Carg11014 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 2.4e-195
Identity = 321/390 (82.31%), Postives = 355/390 (91.03%), Query Frame = 0

Query: 59  SEELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKR 118
           S E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  +RKR
Sbjct: 27  SNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKR 86

Query: 119 LAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQ 178
           LA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRDMVI 
Sbjct: 87  LADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVIT 146

Query: 179 LKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTH 238
           LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRSSP+H
Sbjct: 147 LKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSH 206

Query: 239 FGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLL 298
           +G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNEVMLL
Sbjct: 207 YGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLL 266

Query: 299 GHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 358
           GHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTI
Sbjct: 267 GHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTI 326

Query: 359 NSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYAR 418
           NSQGNR+ AP NPFAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A+YAR
Sbjct: 327 NSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYAR 386

Query: 419 ASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           ASSL AKS+S+V ++TS +G+L CR G  C
Sbjct: 387 ASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Carg11014 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 675.2 bits (1741), Expect = 4.9e-193
Identity = 331/440 (75.23%), Postives = 372/440 (84.55%), Query Frame = 0

Query: 9   SLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSEELNNEHAV 68
           S S LAL+    +    +    ++     L +   SS NSS A +  NDD     NEHAV
Sbjct: 5   SRSFLALSTTLIILALCINSSTMAQETEDLNS--HSSSNSSTANKLPNDDGAW--NEHAV 64

Query: 69  DNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLAECGIGFGR 128
            NP+E+AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH +RKRLA C IGFGR
Sbjct: 65  KNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGR 124

Query: 129 NAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSF 188
           NAIGGRDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSF
Sbjct: 125 NAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSF 184

Query: 189 KTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGD 248
           KTID RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSP+H+GWRTMADGD
Sbjct: 185 KTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGD 244

Query: 249 AISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDK 308
           AISIFGSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK
Sbjct: 245 AISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDK 304

Query: 309 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 368
            MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP
Sbjct: 305 LMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAP 364

Query: 369 TNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSAS 428
            NPFAKEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS
Sbjct: 365 GNPFAKEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPAS 424

Query: 429 MVASITSSAGSLPCRRGHPC 449
           +V+ +T S+G+L CR G  C
Sbjct: 425 VVSMLTYSSGALKCRIGMRC 440

BLAST of Carg11014 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.6e-191
Identity = 322/414 (77.78%), Postives = 360/414 (86.96%), Query Frame = 0

Query: 36  TVLATKFQSSINSSMATRAVNDD-SEELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFF 95
           T +   F S++  SM    V+   S E  +EHAV+NP+E+AA V+MSIRNST RR+LG+F
Sbjct: 4   TQILVVFASALLLSMFFTGVDSTRSNETWHEHAVENPEEVAAMVDMSIRNSTARRRLGYF 63

Query: 96  SCGTGNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKP 155
           SC TGNPIDDCWRCD  W  +RK LA C IGFGRNAIGGRDGR+YVV+D +D++PVNPKP
Sbjct: 64  SCSTGNPIDDCWRCDRRWQSRRKHLANCAIGFGRNAIGGRDGRYYVVSDPNDDNPVNPKP 123

Query: 156 GTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNI 215
           GTLRHAVIQE+PLWIVFKRDMVI LK+ELIMNSFKTID RGVNVHIANGACITIQFVTNI
Sbjct: 124 GTLRHAVIQEEPLWIVFKRDMVITLKEELIMNSFKTIDGRGVNVHIANGACITIQFVTNI 183

Query: 216 IIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVD 275
           IIHG+HIHDC+PTGNAMVRSSP+H+GWRTMADGD ISIFGSSHIWIDHNSLSNCADGL+D
Sbjct: 184 IIHGIHIHDCRPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSNCADGLID 243

Query: 276 AVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGY 335
           AVM STAITISNN+FTHHNEVMLLGHSD+YT+DK MQVTIAYNHFGEGLIQRMPRCRHGY
Sbjct: 244 AVMASTAITISNNYFTHHNEVMLLGHSDTYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGY 303

Query: 336 FHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRS 395
           FHVVNNDYTHWEMYAIGGSA+PTINSQGNRY AP N FAKEVTKR    + +W+ WNWRS
Sbjct: 304 FHVVNNDYTHWEMYAIGGSASPTINSQGNRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRS 363

Query: 396 EGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           EGD+ LNGA+FT SG+G  ASYARASSL AKS+S+V  IT +AG+L CR G  C
Sbjct: 364 EGDLFLNGAFFTRSGSGLGASYARASSLAAKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of Carg11014 vs. ExPASy TrEMBL
Match: A0A6J1HK13 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111464269 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.3e-260
Identity = 447/448 (99.78%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRK+EVSGIRTVLATKFQSSINSSMATRAVNDDSE
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKQEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. ExPASy TrEMBL
Match: A0A6J1KD84 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111494562 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 446/448 (99.55%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSLLALAMVFFVTMALVRKEEVS IRTVLATKFQSSINSSMATRAVNDD E
Sbjct: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSEIRTVLATKFQSSINSSMATRAVNDDLE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA
Sbjct: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMVASITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. ExPASy TrEMBL
Match: A0A6J1CB90 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009920 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.0e-249
Identity = 428/448 (95.54%), Postives = 434/448 (96.88%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALS+WSSLSL+ALAMVFFVTMALVR+E+ S IRT LA +FQSS NSSMA R  NDD E
Sbjct: 1   MAALSRWSSLSLVALAMVFFVTMALVRREQTSEIRTELAAEFQSSSNSSMAARVENDDVE 60

Query: 61  ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLA 120
           +L NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD NWH  RKRLA
Sbjct: 61  KLKNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLA 120

Query: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180
           ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK
Sbjct: 121 ECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLK 180

Query: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240
           QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG
Sbjct: 181 QELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFG 240

Query: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300
           WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH
Sbjct: 241 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGH 300

Query: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360
           SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 301 SDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 360

Query: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420
           QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS
Sbjct: 361 QGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARAS 420

Query: 421 SLGAKSASMVASITSSAGSLPCRRGHPC 449
           SLGAKSASMV SITSSAGSLPCRRGHPC
Sbjct: 421 SLGAKSASMVGSITSSAGSLPCRRGHPC 448

BLAST of Carg11014 vs. ExPASy TrEMBL
Match: A0A5D3CXY1 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001060 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.8e-246
Identity = 425/449 (94.65%), Postives = 433/449 (96.44%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSL ALA++FF+T+ALVRKEE S IRTV A +FQSS +SSMATR  N D E
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  -ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRL 120
            ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD NWH  RKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSP+HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNPFAKEVTKRVET ESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVASITSSAGSLPCRRGHPC 449
           SSLGAKSASMV SITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of Carg11014 vs. ExPASy TrEMBL
Match: A0A1S3CDJ9 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103499488 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.8e-246
Identity = 425/449 (94.65%), Postives = 433/449 (96.44%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MAALSKWSSLSL ALA++FF+T+ALVRKEE S IRTV A +FQSS +SSMATR  N D E
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  -ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRL 120
            ELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD NWH  RKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVI+L
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSP+HF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNPFAKEVTKRVET ESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVASITSSAGSLPCRRGHPC 449
           SSLGAKSASMV SITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

BLAST of Carg11014 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 708.8 bits (1828), Expect = 2.9e-204
Identity = 352/453 (77.70%), Postives = 389/453 (85.87%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSE 60
           MA+ S+      +A+ +V  +T  + R  E+S  R +   +FQS  +S+MA  A   D E
Sbjct: 1   MASSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEEFQSLNSSTMA--ATRLDGE 60

Query: 61  ELNNEHAV-DNP----DEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHK 120
               +HAV D+P    DE+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD NWH  
Sbjct: 61  PQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDM 180

Query: 181 VIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VI+LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSS 240

Query: 241 PTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
           P+HFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHFTHHNEV
Sbjct: 241 PSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 
Sbjct: 301 MLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAE 360

Query: 361 PTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP + FAKEVTKRVET  SEWK WNWRSEGD+LLNGA+F PSGAGASAS
Sbjct: 361 PTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASAS 420

Query: 421 YARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           Y RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 421 YGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 451

BLAST of Carg11014 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 704.9 bits (1818), Expect = 4.1e-203
Identity = 335/410 (81.71%), Postives = 368/410 (89.76%), Query Frame = 0

Query: 43  QSSINSSMATRAVNDDSEELNNEHAVDNPDEIA----ASVEMSIRNSTERRKLGFFSCGT 102
           +++ + ++++   +D++E   N+HAV NPDE+A    A  EMS+RN TERRKLG+F+CGT
Sbjct: 22  ENTTHDNISSLPRSDETEW--NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGT 81

Query: 103 GNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLR 162
           GNPIDDCWRCDPNWH  RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLR
Sbjct: 82  GNPIDDCWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLR 141

Query: 163 HAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHG 222
           HAVIQ++PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HG
Sbjct: 142 HAVIQDRPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHG 201

Query: 223 LHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 282
           LHIHDCKPTGNAMVRSS THFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMG
Sbjct: 202 LHIHDCKPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMG 261

Query: 283 STAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 342
           STAITISNNH THHNEVMLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVV
Sbjct: 262 STAITISNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVV 321

Query: 343 NNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDM 402
           NNDYTHWEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  S WKGWNWRSEGD+
Sbjct: 322 NNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDL 381

Query: 403 LLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           L NGAYFT SGA AS SYARASSL AKS+S+V  ITS AG+LPCRRG  C
Sbjct: 382 LQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Carg11014 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 701.0 bits (1808), Expect = 6.0e-202
Identity = 352/470 (74.89%), Postives = 388/470 (82.55%), Query Frame = 0

Query: 1   MAALSKWSSLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMAT-------- 60
           MA+ S+      +A+ +V  +T  + R  E+S  R +     QSS +S+MA         
Sbjct: 1   MASSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEE 60

Query: 61  ---------RAVNDDSEELNNEHAV-DNP----DEIAASVEMSIRNSTERRKLGFFSCGT 120
                     A   D E    +HAV D+P    DE+A  V+MS +N T RRKLGFFSCGT
Sbjct: 61  FQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWHHKRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLR 180
           GNPIDDCWRCD NWH  RKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLR
Sbjct: 121 GNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLR 180

Query: 181 HAVIQEKPLWIVFKRDMVIQLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHG 240
           HAVIQE+PLWIVFKRDMVI+LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHG
Sbjct: 181 HAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LHIHDCKPTGNAMVRSSP+HFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMG 300

Query: 301 STAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAIT+SNNHFTHHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVV
Sbjct: 301 STAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDM 420
           NNDYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  SEWK WNWRSEGD+
Sbjct: 361 NNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDL 420

Query: 421 LLNGAYFTPSGAGASASYARASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           LLNGA+F PSGAGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 421 LLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of Carg11014 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 682.9 bits (1761), Expect = 1.7e-196
Identity = 321/390 (82.31%), Postives = 355/390 (91.03%), Query Frame = 0

Query: 59  SEELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKR 118
           S E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  +RKR
Sbjct: 27  SNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKR 86

Query: 119 LAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQ 178
           LA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRDMVI 
Sbjct: 87  LADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVIT 146

Query: 179 LKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTH 238
           LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRSSP+H
Sbjct: 147 LKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSH 206

Query: 239 FGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLL 298
           +G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNEVMLL
Sbjct: 207 YGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLL 266

Query: 299 GHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 358
           GHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTI
Sbjct: 267 GHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTI 326

Query: 359 NSQGNRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYAR 418
           NSQGNR+ AP NPFAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A+YAR
Sbjct: 327 NSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYAR 386

Query: 419 ASSLGAKSASMVASITSSAGSLPCRRGHPC 449
           ASSL AKS+S+V ++TS +G+L CR G  C
Sbjct: 387 ASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Carg11014 vs. TAIR 10
Match: AT3G24670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 675.2 bits (1741), Expect = 3.5e-194
Identity = 331/440 (75.23%), Postives = 372/440 (84.55%), Query Frame = 0

Query: 9   SLSLLALAMVFFVTMALVRKEEVSGIRTVLATKFQSSINSSMATRAVNDDSEELNNEHAV 68
           S S LAL+    +    +    ++     L +   SS NSS A +  NDD     NEHAV
Sbjct: 5   SRSFLALSTTLIILALCINSSTMAQETEDLNS--HSSSNSSTANKLPNDDGAW--NEHAV 64

Query: 69  DNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDPNWHHKRKRLAECGIGFGR 128
            NP+E+AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH +RKRLA C IGFGR
Sbjct: 65  KNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGR 124

Query: 129 NAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIQLKQELIMNSF 188
           NAIGGRDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSF
Sbjct: 125 NAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSF 184

Query: 189 KTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGD 248
           KTID RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSP+H+GWRTMADGD
Sbjct: 185 KTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGD 244

Query: 249 AISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDK 308
           AISIFGSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK
Sbjct: 245 AISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDK 304

Query: 309 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 368
            MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP
Sbjct: 305 LMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAP 364

Query: 369 TNPFAKEVTKRVETAESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSAS 428
            NPFAKEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS
Sbjct: 365 GNPFAKEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPAS 424

Query: 429 MVASITSSAGSLPCRRGHPC 449
           +V+ +T S+G+L CR G  C
Sbjct: 425 VVSMLTYSSGALKCRIGMRC 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6593793.11.2e-260100.00putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_022964161.12.6e-26099.78probable pectate lyase 8 [Cucurbita moschata] >XP_022964162.1 probable pectate l... [more]
XP_023515191.12.2e-25999.55probable pectate lyase 8 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023000287.15.0e-25999.55probable pectate lyase 8 [Cucurbita maxima][more]
XP_022138849.16.1e-24995.54probable pectate lyase 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q940Q15.8e-20281.71Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q944R18.4e-20174.89Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q9M8Z82.4e-19582.31Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1[more]
Q9LJ424.9e-19375.23Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2[more]
Q93WF11.6e-19177.78Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HK131.3e-26099.78Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111464269 PE=3 SV=1[more]
A0A6J1KD842.4e-25999.55Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111494562 PE=3 SV=1[more]
A0A6J1CB903.0e-24995.54Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009920 PE=3 SV=1[more]
A0A5D3CXY16.8e-24694.65Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001060 ... [more]
A0A1S3CDJ96.8e-24694.65Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103499488 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G13710.22.9e-20477.70Pectin lyase-like superfamily protein [more]
AT1G04680.14.1e-20381.71Pectin lyase-like superfamily protein [more]
AT4G13710.16.0e-20274.89Pectin lyase-like superfamily protein [more]
AT3G07010.11.7e-19682.31Pectin lyase-like superfamily protein [more]
AT3G24670.13.5e-19475.23Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 122..139
score: 54.76
coord: 348..367
score: 75.0
coord: 146..171
score: 65.75
coord: 390..414
score: 53.71
coord: 182..198
score: 64.99
coord: 326..345
score: 78.1
coord: 419..442
score: 35.91
coord: 246..267
score: 69.48
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 174..371
e-value: 2.5E-105
score: 365.9
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 186..363
e-value: 4.1E-19
score: 69.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 99..441
e-value: 1.2E-118
score: 398.0
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 18..445
NoneNo IPR availablePANTHERPTHR31683:SF81PECTATE LYASEcoord: 18..445
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 76..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11014-RACarg11014-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity