Carg10993 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10993
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit B
LocationCarg_Chr08: 6252647 .. 6255157 (-)
RNA-Seq ExpressionCarg10993
SyntenyCarg10993
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACCCAACAGTCAGGGAGGTTGAGATTCTCGTTTAGGAATGCGACCGTAGCTGTGTGCTTGTTGAACCTGGTGGCGGCTTTTCTTATGCTTCAGGGTTTCTTGTTTGCTGCCTCTTCCCGCAATAGATCCTCCTCAACAAACCCTAATTTGGGTTCGTTTCCTTCACCTCTCTGGACTTTTCTCTTCATTGCTTTTCTGATTTCTGCAAATTCTTTGTGAAGTAATCTAAATATTTGTTGTTTTCTTAGGTTTGATTTTTAGTGCATCAAACTGATTCTAATGAATGTTTGTGCCGCTGATCAGAGAGATGCTAGGGTGATATTTTGAACTGACTATGCTTGTTGGTGGATTGCTTAGAAGAGGGCTTAGGTATCATGAACTACAAGAAAACAAAAAGTTGTGTATATGACTGTTGGGGATTGTTGGGAGGGAGTAAGGGGGTGGATTGTGAGATCCCACATCGATTAGAGAAGGGAACGAATGTTAGCGAGAACACTCCGAATGGGGGTTGATTGTGAGATCCCACATCGATTGGATAAGGGAACGAATGTTAGCAAGAATGCTGGTTCCGTAGGGGGGGTGAATTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAGCATTTTTTGTAAGGGGTGTGGAAACCTCTCCCTAGCAGACATGTTTTAAAACCTTGAGGGGAAGCCTCGAAAGGGAAAGCTTAAAGAGGATAATTTATGCTAGTGGTGGGTTTGGGCCGTTACAAATGGTATCAGAGCCAAACACCGGGGGGTGTGCCAGCGAGGACATTGGCCCCCAAGGGGGGTGGATTGTGAGATCCCACGTTGGTTGGAGAGGAGAAGGAATGAAACATTTTTTATAAGAGTGTGGAAACCTCTCCCCAGTAGACGCGTTTTAAAACCTTGAGGGGAAGGCTGAAAGGGAAAGCCCAAAGAGGAGAATATCTCCTCGCGGTGGACTTGGGCTGTTACATCCACATTGGCTAATTAGGGGAGTGATCATGAATTTATAAGTACGAGGCCTTTTGGGGAAACCAAAAGTAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGATTCGTGATTCCTAACACTGAGGACGTAGTCTTTAAACCCATTTAGCCAAATCCGAAGAATGCTAACATTGATGGATTTGATGACAACGGCAACCGTTGTTTTGATTACCTGCACCAATAGAGTGAAGATGATCTGGTTTGGGAAACACGTTTATAACATCAAGATGGCTGTTAGGAGAGCATAATGATAATTAGTTAGGAGAGCTTGGTTATATAGATCTTAGAAGGGGGGAAGATTTTGGGCATTGTATAGCGTGGGACTTTGGGAGAGACATAACCTGTTTGGAGTCACGACCCTCTACAATGGTATGATATTGTCCACTTTGAGCATAAGCACTCGTAGCTTTGATTTTGGTTTCCCGAAAATGTCTCATACCAGTGGAGATGTATTCCTTACTTATAAATCCGTGATCATTCCGTTAATTAGCGAATGTGGGACTCCCTCCCAACCATCTTCGACATGGTGAAGCTTGAAGGGGGGGGATATATTGTTTGTCTAAATGTACTTATTAACTGAACTTTTTAATGGAAAAAAATTGTTGTCTGAATTGTTCAGCCCTCCTTTGTTTATGCTCTATACTTCTTAACTGGCTTGCTAACTTAGATAATGGGTGAAATTTCATACTGCAGATCAACTGAAATATATCAGGGAATCCGAAGAGATTCGACTTGCCATGCAGCCATTGGAACTGATTAAAAGGGTAAGCGTCTCGAGTATATCCTATAACCCATCAAAGCACAAGACATAAGTCTCATGAGTAAATAAAAACCGAACCGAACCTTGTCTAAGCACAAGAAAGAAACGACAACGATGGCTGAAAAATCGTTTAAGCAAACTTATGAATTGAATTAACGAATGGAGATGATTGGGTTTAATAAGTTTTTCGTTTGAGAGGAGAATGAAGCATTCTTAATAAGGGTGTGGAAACCTCTCCCATGTGTTTTAAAAATCTTGTGGGGAAGTCCGAAAGGAAAAGTTCAAAGAGGACAATATAAATGATATTAGAACCAGACATCGGGCGGTGTGTCAGTGAGGACGTTGGGCCCCGAAAGAGGAATGGATTGTGAGATCCCACATTGATTGGAGAGAGGAATGAGTGTGAACAAGAACGCTGAGCCGGGGGCTGGATTGTGAGATCCCACATCAGTTGGAGAGGAGAACAAAGCATAGGATTAGAGGTTCGAGTCTTCTACCTCACATGTCCTTGAGGATTTTGTTTTTAAAGAATAAAGTTGAGTCGACTTGTTTACACACGGACCGCCATCCCGTGTCTGAGCTAATATGATATGAACGATTTGTGAATTGTGACACAGCTTTGGAGGAATGGCGGAAAAGGAAGATGGAAAGAGCAAGAGTGCGAGAAATGGAGAAAAATGGGACACTGAGATCCCCAACTTCTAAATCTGTATGA

mRNA sequence

ATGGAGACCCAACAGTCAGGGAGGTTGAGATTCTCGTTTAGGAATGCGACCGTAGCTGTGTGCTTGTTGAACCTGGTGGCGGCTTTTCTTATGCTTCAGGGTTTCTTGTTTGCTGCCTCTTCCCGCAATAGATCCTCCTCAACAAACCCTAATTTGGCTTTGGAGGAATGGCGGAAAAGGAAGATGGAAAGAGCAAGAGTGCGAGAAATGGAGAAAAATGGGACACTGAGATCCCCAACTTCTAAATCTGTATGA

Coding sequence (CDS)

ATGGAGACCCAACAGTCAGGGAGGTTGAGATTCTCGTTTAGGAATGCGACCGTAGCTGTGTGCTTGTTGAACCTGGTGGCGGCTTTTCTTATGCTTCAGGGTTTCTTGTTTGCTGCCTCTTCCCGCAATAGATCCTCCTCAACAAACCCTAATTTGGCTTTGGAGGAATGGCGGAAAAGGAAGATGGAAAGAGCAAGAGTGCGAGAAATGGAGAAAAATGGGACACTGAGATCCCCAACTTCTAAATCTGTATGA

Protein sequence

METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLALEEWRKRKMERARVREMEKNGTLRSPTSKSV
Homology
BLAST of Carg10993 vs. NCBI nr
Match: KAG7026142.1 (hypothetical protein SDJN02_12641, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 158.7 bits (400), Expect = 2.2e-35
Identity = 84/84 (100.00%), Postives = 84/84 (100.00%), Query Frame = 0

Query: 1  METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLALEEWRKR 60
          METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLALEEWRKR
Sbjct: 1  METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLALEEWRKR 60

Query: 61 KMERARVREMEKNGTLRSPTSKSV 85
          KMERARVREMEKNGTLRSPTSKSV
Sbjct: 61 KMERARVREMEKNGTLRSPTSKSV 84

BLAST of Carg10993 vs. NCBI nr
Match: XP_022964013.1 (uncharacterized protein LOC111464164 [Cucurbita moschata])

HSP 1 Score: 127.9 bits (320), Expect = 4.2e-26
Identity = 84/153 (54.90%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL        
Sbjct: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 85
                                                                       
Sbjct: 61  SEEIRLAMQPLELIKRVREIQREAYYESEDIQEKDSKQNAAIDLSKRLKDFRSLNDASNL 120

BLAST of Carg10993 vs. NCBI nr
Match: XP_023000555.1 (uncharacterized protein LOC111494796 [Cucurbita maxima] >XP_023513583.1 uncharacterized protein LOC111778147 [Cucurbita pepo subsp. pepo] >KAG6593808.1 hypothetical protein SDJN03_13284, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 127.9 bits (320), Expect = 4.2e-26
Identity = 84/153 (54.90%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL        
Sbjct: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 85
                                                                       
Sbjct: 61  SEEIRLAMQPLELIKRVREIQQEAYYESEDIQEKDSKQNAAIDLSKRLKDFRSLNDASNL 120

BLAST of Carg10993 vs. NCBI nr
Match: XP_022138362.1 (uncharacterized protein LOC111009555 [Momordica charantia])

HSP 1 Score: 97.4 bits (241), Expect = 6.1e-17
Identity = 66/149 (44.30%), Postives = 73/149 (48.99%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           ME QQ  RLRFSF+NAT+A+CLLNLVAAF +LQGFL AASSRNR SSTNP+L        
Sbjct: 1   MEVQQPWRLRFSFKNATIAMCLLNLVAAFFLLQGFLSAASSRNRPSSTNPSLGQLKYIRE 60

Query: 61  ------------------------------------------------------------ 81
                                                                       
Sbjct: 61  SEEIRFAMQPLELIKRVREIEREAHYETEVVQEKDSKQNAAIDLSKRLKDFRSLNDASSL 120

BLAST of Carg10993 vs. NCBI nr
Match: XP_038874445.1 (uncharacterized protein LOC120067104 [Benincasa hispida] >XP_038874446.1 uncharacterized protein LOC120067104 [Benincasa hispida])

HSP 1 Score: 95.5 bits (236), Expect = 2.3e-16
Identity = 67/147 (45.58%), Postives = 71/147 (48.30%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           ME QQS RLRFSFRNAT+A+CLLNLVA FL+LQGFL AASSRNR SSTN NL        
Sbjct: 1   MEVQQSWRLRFSFRNATIALCLLNLVAVFLLLQGFLSAASSRNRPSSTNLNLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 79
                                                                       
Sbjct: 61  SEEIRLSMQPLELIKRVREIQQEAHYETEDVQDKDSKQNAAIDLSKRLKDFRSLNDASSL 120

BLAST of Carg10993 vs. ExPASy TrEMBL
Match: A0A6J1KIP3 (uncharacterized protein LOC111494796 OS=Cucurbita maxima OX=3661 GN=LOC111494796 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-26
Identity = 84/153 (54.90%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL        
Sbjct: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 85
                                                                       
Sbjct: 61  SEEIRLAMQPLELIKRVREIQQEAYYESEDIQEKDSKQNAAIDLSKRLKDFRSLNDASNL 120

BLAST of Carg10993 vs. ExPASy TrEMBL
Match: A0A6J1HJK6 (uncharacterized protein LOC111464164 OS=Cucurbita moschata OX=3662 GN=LOC111464164 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-26
Identity = 84/153 (54.90%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL        
Sbjct: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 85
                                                                       
Sbjct: 61  SEEIRLAMQPLELIKRVREIQREAYYESEDIQEKDSKQNAAIDLSKRLKDFRSLNDASNL 120

BLAST of Carg10993 vs. ExPASy TrEMBL
Match: A0A6J1CAW5 (uncharacterized protein LOC111009555 OS=Momordica charantia OX=3673 GN=LOC111009555 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.9e-17
Identity = 66/149 (44.30%), Postives = 73/149 (48.99%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           ME QQ  RLRFSF+NAT+A+CLLNLVAAF +LQGFL AASSRNR SSTNP+L        
Sbjct: 1   MEVQQPWRLRFSFKNATIAMCLLNLVAAFFLLQGFLSAASSRNRPSSTNPSLGQLKYIRE 60

Query: 61  ------------------------------------------------------------ 81
                                                                       
Sbjct: 61  SEEIRFAMQPLELIKRVREIEREAHYETEVVQEKDSKQNAAIDLSKRLKDFRSLNDASSL 120

BLAST of Carg10993 vs. ExPASy TrEMBL
Match: A0A5D3D0K8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G00180 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.9e-16
Identity = 66/149 (44.30%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           ME QQS RLRFSFRNAT+A+CLLNLVAAFL+LQGFL AASSR+R  STN  L        
Sbjct: 1   MEVQQSWRLRFSFRNATIALCLLNLVAAFLLLQGFLSAASSRSRPLSTNQKLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 81
                                                                       
Sbjct: 61  AQEIRLSMQPLELIKRVREIQEEAYYETEAVQDKDSKQNAAIDLSKRLKDFRSLNDASSL 120

BLAST of Carg10993 vs. ExPASy TrEMBL
Match: A0A5A7SNT0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold134G00220 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.5e-16
Identity = 65/149 (43.62%), Postives = 71/149 (47.65%), Query Frame = 0

Query: 1   METQQSGRLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL-------- 60
           ME QQS RLRFSFRNAT+A+C LNLVAAFL+LQGFL AASSR+R  STN  L        
Sbjct: 1   MEVQQSWRLRFSFRNATIALCFLNLVAAFLLLQGFLSAASSRSRPLSTNQKLDQLKYIRE 60

Query: 61  ------------------------------------------------------------ 81
                                                                       
Sbjct: 61  AQEIRLSMQPLELIKRVREIQEEAYYETEAVQDKDSKQNAAIDLSKRLKDFRSLNDASSL 120

BLAST of Carg10993 vs. TAIR 10
Match: AT1G04555.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G33380.1); Has 84 Blast hits to 83 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 9; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 45.4 bits (106), Expect = 2.5e-05
Identity = 40/146 (27.40%), Postives = 57/146 (39.04%), Query Frame = 0

Query: 8   RLRFSFRNATVAVCLLNLVAAFLMLQGFLFAASSRNRSSSTNPNL--------------- 67
           R+RFSF+NAT+A+ ++N++    +LQGF  ++SS + SSS+   +               
Sbjct: 6   RMRFSFKNATIALTVINVLIFLFLLQGFFTSSSSPSSSSSSRRLISAQLRYIKEAEELRL 65

Query: 68  -------------------------------------------------------ALEEW 84
                                                                  ALEEW
Sbjct: 66  KMQPLELIRRVREIEQEASAGQETEQQKDVKQTTAVDLSKRLKDFRALNDASSLKALEEW 125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026142.12.2e-35100.00hypothetical protein SDJN02_12641, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022964013.14.2e-2654.90uncharacterized protein LOC111464164 [Cucurbita moschata][more]
XP_023000555.14.2e-2654.90uncharacterized protein LOC111494796 [Cucurbita maxima] >XP_023513583.1 uncharac... [more]
XP_022138362.16.1e-1744.30uncharacterized protein LOC111009555 [Momordica charantia][more]
XP_038874445.12.3e-1645.58uncharacterized protein LOC120067104 [Benincasa hispida] >XP_038874446.1 unchara... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1KIP32.0e-2654.90uncharacterized protein LOC111494796 OS=Cucurbita maxima OX=3661 GN=LOC111494796... [more]
A0A6J1HJK62.0e-2654.90uncharacterized protein LOC111464164 OS=Cucurbita moschata OX=3662 GN=LOC1114641... [more]
A0A6J1CAW52.9e-1744.30uncharacterized protein LOC111009555 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A5D3D0K81.9e-1644.30Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7SNT05.5e-1643.62Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
AT1G04555.12.5e-0527.40unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10993-RACarg10993-RAmRNA