Carg10958 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10958
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWAT1-related protein
LocationCarg_Chr08: 6434362 .. 6437066 (-)
RNA-Seq ExpressionCarg10958
SyntenyCarg10958
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGAACGTCCGGTTAGGAGTGTTTTTCACTAGGTTTAAACCACATATCCTCGTAATTTTTGCTCAGGTTGGCTATACATTTATGTATTTCTTCACGGATGCTTCCTTCAAGCATGGCATGAATCCTCACGTCCATATCACTTATCGACAAATCGTTGCTAGCGTTGGGATGTTTCCCCTCGCTTATTTCTTTGAAAGGTTCCATGCTAATTATCTTCTCAAAGTTTCATGCTTTATTTGGCGTATTAGATTATTTTAAAATATATGTTGGTTTACGTAGAAAAACAAGACCGAGGATGACGGTGACACTATTCTTGGAAATTTTTGTTCTTTCGCTTTTAGGGTAAGCTTTTGAATCATCATTTAATCCATTTTAATACGTAAATTTTAAAGATCGAATAATGAAATACAGGATGAGTTTGAGTCTCAACATGCACTTTGCAAGCTTGACATACTCATCCCCCACGTTCGTTACATCATCGATCAACACGATAGCGTGTTTGACGTTCATAATTGCAGTCATGTTCAGGTTCTTACACGTCTATGTATATCGACAAGAAACAAAAATGTTATCAAACAAGTCGTGACGTTGTTTTGATTTATATGGGCAGAATGGAAATTGTTGACCTTAAAAATCCAAGAGGGATAGCAAAAGTTGTGGGGACATTGGTTTCATTGGGTGGTGTGATGATAATGACTTTTTATAAAGGACCCATCATCAGAAATCTATGGCATCCTTTCATCCATATTCAACACAAAGCTTCTAATCTCCATGAGAATTGGCTCAAGGGTTCACTTCTTACTGTTTCAAGCTGCATTTCTTGGGCCCTCTCCTACATTATGCAGGTCCCTAAACCCTAATTTTTCTAATGATCGACTTTCCATGCACGTACTGATCAGTGTCGTTACTTACAAACAAGACCTTTTTTCGATATATTCTAATGTAATCTATTGTGGGGTGGAATTGAAGGCATTTACATTGAAACGATACCCGGCGCAGCTATCGTTATCAACGTGGATGAACTTGGTCGGAGCAGCGCAATCGGGTGTGTTTGCAGTGTTGACACAACACAAGCCAGGAGTTTGGAGTGTTGGACTTAACATTGATCTTTGGTGCATTATCTATTCTGTAAGAAACAGACCAAAAGCAACTTTCATTAGGTTTAATGGGTATTAAAAATTGAAAGGCCTTTAGGTTTTGATGGTATGAGTTGAATTGTTGCAGGGAATTGTGTGCTCAGCTTTGACAGTATACATACAACTTTGGTGCACAGAAGAAAAAGGCCCAGTTTTTGTGACCATGTTCGATCCACTTTGTACAGTTTTGGTGGCTGCTTTAGCTTACTTTGTTTTTGGACAAAAGCTCTACATGGGGAGGTAAGAAAATTCAATCTACAACAAGAATATGATATGAAACATGATAGAAAAATGATCAAATTATAAATTTTAAGAGTTCGAGAATTTATCGATCTTCTTCATCAGCGGTTTTTCCTGTGTCAATAGAGCTAGATGTGAATCGAGTAAGCTTGTTGGAAAACGGGCCTTTTCTTTTCTTCTCCCTTCTTTTGTCCGAACATAATACTTTGAGATCTTACATCGGTTGGAGAGAGGAACGATTATATTCCTTATAAAGGTGTAGAAACCTCTCCTTAGTGGACGCGTTTTAAAACCTTGAGGGGAAGCACGAAACGGAAAGCCAAAAGAGGACAATATTGGCTGGGAGTGACCTTGGGCAGTTACAAATGATATCAGAGTGAAACAACGGGCGGTATGCCAGCGAGGACGTTGGGGACCCCAAGAGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGGGAATTAAACATTCCTTCATTCCTCGTAAGGGTGTGGAATCCTCTCCCTAGTGGACGCATTTTAAAACCTTGAGGGGAACCCTGAAAGGAAAGCTTGAAAGGGAAAGTCCAAAGAGAACAATATCGGCTAGTGGTGGCCTTGGGTAGTTACAAATGGTATCAGAACGAAACTCCAGGGGGTGTGCCAGCGAGGATGCTGGAGTCACCAAGAGGGGTGGATTGTGAGATCCCACATCGGTTAGAGAGGAGAATGAAACATTCCTTCATTTCTTATAAGGTGTGAAATCCTCTCCACGGTGGATGCATTTTAAAACTTTGAGGGGAAGCCCGAAAGGGAAAGTCCAAAGAGGACAATACTTAGTAGTGGTGGCCTTGGACCGTTAAAAATGGTATCAAAGCGAAACATCGAGCGGTGTGCTAGCGAAAACGCTAGATCCTCAAGTGGGGTGGATTGTAAGATCCTACATCGGTTGGAGATGGAAACCTTTTCAGAGTGGACAAGTTTTAAAATTTTGAGGGAAATCTCAAAAAGGACAGCCACGGTGGCCTTGGGCCATTACAAACACGGGAAGTACAGAATCGGATGAATTTCTTTGATCGAATTCTTTTAGAGTTGAAAGTATGATTGATATAGCAATTTCTGAAACTTGTTTTTTGGTAATGTTGTAATTGTGTAGCATTGTTGGTGGAGGCATCGTGATTATGGGGCTGTATATGCTGTTATGGGGCAAAGAACATGATAAGTTCGAGCTCCAAAATAAGTCACAATTGGAATCTGATTCAGTGCATGAAACTTTCAACCAACCTGATCTGCAAATTAGTGCATCACCACCCCCTAACCATGTTCTACAACATCAACCTTAG

mRNA sequence

ATGGCGATGAACGTCCGGTTAGGAGTGTTTTTCACTAGGTTTAAACCACATATCCTCGTAATTTTTGCTCAGGTTGGCTATACATTTATGTATTTCTTCACGGATGCTTCCTTCAAGCATGGCATGAATCCTCACGTCCATATCACTTATCGACAAATCGTTGCTAGCGTTGGGATGTTTCCCCTCGCTTATTTCTTTGAAAGAATGGAAATTGTTGACCTTAAAAATCCAAGAGGGATAGCAAAAGTTGTGGGGACATTGGTTTCATTGGGTGGTGTGATGATAATGACTTTTTATAAAGGACCCATCATCAGAAATCTATGGCATCCTTTCATCCATATTCAACACAAAGCTTCTAATCTCCATGAGAATTGGCTCAAGGGTTCACTTCTTACTGTTTCAAGCTGCATTTCTTGGGCCCTCTCCTACATTATGCAGGCATTTACATTGAAACGATACCCGGCGCAGCTATCGTTATCAACGTGGATGAACTTGGTCGGAGCAGCGCAATCGGGTGTGTTTGCAGTGTTGACACAACACAAGCCAGGAGTTTGGAGTGTTGGACTTAACATTGATCTTTGGTGCATTATCTATTCTGGAATTGTGTGCTCAGCTTTGACAGTATACATACAACTTTGGTGCACAGAAGAAAAAGGCCCAGTTTTTGTGACCATGTTCGATCCACTTTGTACAGTTTTGGTGGCTGCTTTAGCTTACTTTGTTTTTGGACAAAAGCTCTACATGGGGAGCATTGTTGGTGGAGGCATCGTGATTATGGGGCTGTATATGCTGTTATGGGGCAAAGAACATGATAAGTTCGAGCTCCAAAATAAGTCACAATTGGAATCTGATTCAGTGCATGAAACTTTCAACCAACCTGATCTGCAAATTAGTGCATCACCACCCCCTAACCATGTTCTACAACATCAACCTTAG

Coding sequence (CDS)

ATGGCGATGAACGTCCGGTTAGGAGTGTTTTTCACTAGGTTTAAACCACATATCCTCGTAATTTTTGCTCAGGTTGGCTATACATTTATGTATTTCTTCACGGATGCTTCCTTCAAGCATGGCATGAATCCTCACGTCCATATCACTTATCGACAAATCGTTGCTAGCGTTGGGATGTTTCCCCTCGCTTATTTCTTTGAAAGAATGGAAATTGTTGACCTTAAAAATCCAAGAGGGATAGCAAAAGTTGTGGGGACATTGGTTTCATTGGGTGGTGTGATGATAATGACTTTTTATAAAGGACCCATCATCAGAAATCTATGGCATCCTTTCATCCATATTCAACACAAAGCTTCTAATCTCCATGAGAATTGGCTCAAGGGTTCACTTCTTACTGTTTCAAGCTGCATTTCTTGGGCCCTCTCCTACATTATGCAGGCATTTACATTGAAACGATACCCGGCGCAGCTATCGTTATCAACGTGGATGAACTTGGTCGGAGCAGCGCAATCGGGTGTGTTTGCAGTGTTGACACAACACAAGCCAGGAGTTTGGAGTGTTGGACTTAACATTGATCTTTGGTGCATTATCTATTCTGGAATTGTGTGCTCAGCTTTGACAGTATACATACAACTTTGGTGCACAGAAGAAAAAGGCCCAGTTTTTGTGACCATGTTCGATCCACTTTGTACAGTTTTGGTGGCTGCTTTAGCTTACTTTGTTTTTGGACAAAAGCTCTACATGGGGAGCATTGTTGGTGGAGGCATCGTGATTATGGGGCTGTATATGCTGTTATGGGGCAAAGAACATGATAAGTTCGAGCTCCAAAATAAGTCACAATTGGAATCTGATTCAGTGCATGAAACTTTCAACCAACCTGATCTGCAAATTAGTGCATCACCACCCCCTAACCATGTTCTACAACATCAACCTTAG

Protein sequence

MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISASPPPNHVLQHQP
Homology
BLAST of Carg10958 vs. NCBI nr
Match: XP_022930408.1 (WAT1-related protein At4g08300-like isoform X3 [Cucurbita moschata] >XP_023514005.1 WAT1-related protein At4g08300-like isoform X3 [Cucurbita pepo subsp. pepo] >KAG7026175.1 WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120
           PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN
Sbjct: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120

Query: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180

Query: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240
           KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240

Query: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS
Sbjct: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300

Query: 301 PPPNHVLQHQP 312
           PPPNHVLQHQP
Sbjct: 301 PPPNHVLQHQP 311

BLAST of Carg10958 vs. NCBI nr
Match: XP_022930406.1 (WAT1-related protein At4g08290-like isoform X2 [Cucurbita moschata] >XP_023514004.1 WAT1-related protein At4g08290-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.5 bits (1599), Expect = 7.6e-174
Identity = 311/332 (93.67%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFER---------------------MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120
           PLAYFFER                     MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY
Sbjct: 61  PLAYFFERKTRPRMTVTLFLEIFVLSLLGMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120

Query: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180
           KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL
Sbjct: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180

Query: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240
           STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG
Sbjct: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240

Query: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300
           PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS
Sbjct: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300

Query: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 312
           QLESDSVHETFNQPDLQISASPPPNHVLQHQP
Sbjct: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 332

BLAST of Carg10958 vs. NCBI nr
Match: XP_023000514.1 (WAT1-related protein At4g08300-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 611.3 bits (1575), Expect = 4.6e-171
Identity = 300/311 (96.46%), Postives = 304/311 (97.75%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHIL+IFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120
           PLAYFFERMEIVDLKNPRGIAKVVGT VSLGGVMIMTFYKGPIIRN WHPFIHIQHKASN
Sbjct: 61  PLAYFFERMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFYKGPIIRNPWHPFIHIQHKASN 120

Query: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ+SLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISLSTWMNLVGAAQSGVFAVLTQH 180

Query: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240
           +PGVWSVGLNIDLWCIIYSGIVCSAL VYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 181 RPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240

Query: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKE D+FELQNKSQLESDSVHETF QPDLQI AS
Sbjct: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKSQLESDSVHETFQQPDLQIGAS 300

Query: 301 PPPNHVLQHQP 312
           PP NHVLQHQP
Sbjct: 301 PPTNHVLQHQP 311

BLAST of Carg10958 vs. NCBI nr
Match: XP_022930405.1 (WAT1-related protein At4g08300-like isoform X1 [Cucurbita moschata] >XP_023514002.1 WAT1-related protein At4g08300-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605.5 bits (1560), Expect = 2.5e-169
Identity = 311/371 (83.83%), Postives = 311/371 (83.83%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFE----------------------------------------------------- 120
           PLAYFFE                                                     
Sbjct: 61  PLAYFFERKTRPRMTVTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSSINTIACLT 120

Query: 121 -------RMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 180
                  RMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN
Sbjct: 121 FIIAVMFRMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 180

Query: 181 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 240
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 181 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 240

Query: 241 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 300
           KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 241 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 300

Query: 301 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 312
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS
Sbjct: 301 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 360

BLAST of Carg10958 vs. NCBI nr
Match: XP_023000513.1 (WAT1-related protein At4g08290-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 599.0 bits (1543), Expect = 2.4e-167
Identity = 300/332 (90.36%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHIL+IFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFER---------------------MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120
           PLAYFFER                     MEIVDLKNPRGIAKVVGT VSLGGVMIMTFY
Sbjct: 61  PLAYFFERKTRPRMTGTLFLEIFVLSLLGMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFY 120

Query: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180
           KGPIIRN WHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ+SL
Sbjct: 121 KGPIIRNPWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISL 180

Query: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240
           STWMNLVGAAQSGVFAVLTQH+PGVWSVGLNIDLWCIIYSGIVCSAL VYIQLWCTEEKG
Sbjct: 181 STWMNLVGAAQSGVFAVLTQHRPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKG 240

Query: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300
           PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKE D+FELQNKS
Sbjct: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKS 300

Query: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 312
           QLESDSVHETF QPDLQI ASPP NHVLQHQP
Sbjct: 301 QLESDSVHETFQQPDLQIGASPPTNHVLQHQP 332

BLAST of Carg10958 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.0e-49
Identity = 114/340 (33.53%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + +P++L+IF Q G    Y    A+   G N +V I YR +VA++ + P A  FE     
Sbjct: 10  KLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRP 69

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  RME V
Sbjct: 70  KMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKV 129

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLW-HPFIHIQHKASNL---HENWLKG 192
           ++   R  AK++GTLV LGG ++MT YKGP+I   W +P +  Q+  +N    H NW+ G
Sbjct: 130 NIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVG 189

Query: 193 SLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSVG 252
           +LL +  C++W+  Y++Q+ T+K YPA LSLS  + L GA QS   A++ +  P  W+VG
Sbjct: 190 TLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVG 249

Query: 253 LNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYM 289
            +  L+  +Y+GIV S +T Y+Q    + +GPVFVT F+PLC +LVA +A F+  ++++ 
Sbjct: 250 WDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHF 309

BLAST of Carg10958 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-46
Identity = 105/325 (32.31%), Postives = 158/325 (48.62%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + KP I +I  Q GY  MY  T  SFKHGMN  +  TYR +VA++ + P A   E     
Sbjct: 8   KLKPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRP 67

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  R+E V
Sbjct: 68  KMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETV 127

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIR--NLWHPFIH---IQHKASNLHENWLK 192
           +LK  R +AKV+GT +++GG M+MT YKGP I      H  +H       +    +NW+ 
Sbjct: 128 NLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVT 187

Query: 193 GSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSV 252
           G+L  + S  +WA  +I+Q+FTLK+YPA+LSL  W+  +G   + + +++       W V
Sbjct: 188 GTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKV 247

Query: 253 GLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLY 273
           G++      +YSG+VCS +  YIQ     E+GPVF T F P+C ++ A L   V  +K++
Sbjct: 248 GMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIH 307

BLAST of Carg10958 vs. ExPASy Swiss-Prot
Match: F4IQX1 (WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.8e-46
Identity = 109/314 (34.71%), Postives = 166/314 (52.87%), Query Frame = 0

Query: 16  PHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE-------- 75
           P IL++  Q+GY  M   T      GM+ +V   YR  VA+V M P A++F+        
Sbjct: 9   PFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDNPVIAQNL 68

Query: 76  -------------------------------RMEIVDLKNPRGIAKVVGTLVSLGGVMIM 135
                                          R+E V  ++ R  AKVVGT+ ++GG+M+M
Sbjct: 69  FNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIMVM 128

Query: 136 TFYKGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ 195
           T  KGP +   W      Q+       + +KG++L    C S+A   I+QA TLK YPA+
Sbjct: 129 TLVKGPALDLFWTKGPSAQNTVGTDIHSSIKGAVLVTIGCFSYACFMILQAITLKTYPAE 188

Query: 196 LSLSTWMNLVGAAQSGVFA-VLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCT 255
           LSL+TW+ L+G  +  V A V+ +  P VW++G +  L  I YSGIVCSAL  YI     
Sbjct: 189 LSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGGVVM 248

Query: 256 EEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFEL 290
           + +GPVFVT F PLC ++VA ++  +F +++Y+G  +G  ++ +GLY+++WGK  D +E 
Sbjct: 249 KTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKD-YEY 308

BLAST of Carg10958 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 102/322 (31.68%), Postives = 160/322 (49.69%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + KP + +I  Q GY  MY  T  SFKHGM+  V  TYR +VA+V M P A  FE     
Sbjct: 8   KIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRP 67

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  R+E V
Sbjct: 68  KMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETV 127

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIR--NLWHPFIHIQHKASNLHENWLKGSL 192
           + +    +AKVVGT++++GG MIMT YKGP I      H   H    ++   ++W+ G++
Sbjct: 128 NFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSSTPTGQHWVLGTI 187

Query: 193 LTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSVGLN 252
             + S  +WA  +I+Q++TLK YPA+LSL T +  +G   + + +++    P  W +G++
Sbjct: 188 AIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMD 247

Query: 253 IDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGS 273
                 +YSG+VCS +  YIQ    +++GPVF T F P+C ++ A L   V  +K+++GS
Sbjct: 248 SGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGS 307

BLAST of Carg10958 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 105/334 (31.44%), Postives = 162/334 (48.50%), Query Frame = 0

Query: 15  KPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE------- 74
           KP++ +I  Q GY  MY  T  S KHGMN +V   YR  +A+  + P A F E       
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 75  -----------------------------------------------------RMEIVDL 134
                                                                R+E V+ 
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 135 KNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFI----------------HIQHKA 194
           K  R IAKVVGT++++ G ++MT YKGPI+      FI                H    A
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIV-----DFIRFGGGGGGGSDGAGGSHGGAGA 189

Query: 195 SNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLT 254
           + + ++W+ G+L+ +     WA  +I+Q+FTLK+YPA+LSL+T + L+G  +    +++T
Sbjct: 190 AAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVT 249

Query: 255 QHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALA 273
                 W +G + +L+   YSG++CS +  Y+Q     E+GPVFV  F+PLC V+ AAL 
Sbjct: 250 VRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALG 309

BLAST of Carg10958 vs. ExPASy TrEMBL
Match: A0A6J1EV31 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.2e-178
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120
           PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN
Sbjct: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120

Query: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180

Query: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240
           KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240

Query: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS
Sbjct: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300

Query: 301 PPPNHVLQHQP 312
           PPPNHVLQHQP
Sbjct: 301 PPPNHVLQHQP 311

BLAST of Carg10958 vs. ExPASy TrEMBL
Match: A0A6J1EQV0 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.7e-174
Identity = 311/332 (93.67%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFER---------------------MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120
           PLAYFFER                     MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY
Sbjct: 61  PLAYFFERKTRPRMTVTLFLEIFVLSLLGMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120

Query: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180
           KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL
Sbjct: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180

Query: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240
           STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG
Sbjct: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240

Query: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300
           PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS
Sbjct: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300

Query: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 312
           QLESDSVHETFNQPDLQISASPPPNHVLQHQP
Sbjct: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 332

BLAST of Carg10958 vs. ExPASy TrEMBL
Match: A0A6J1KK76 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111494761 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.2e-171
Identity = 300/311 (96.46%), Postives = 304/311 (97.75%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHIL+IFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFERMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 120
           PLAYFFERMEIVDLKNPRGIAKVVGT VSLGGVMIMTFYKGPIIRN WHPFIHIQHKASN
Sbjct: 61  PLAYFFERMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFYKGPIIRNPWHPFIHIQHKASN 120

Query: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 180
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ+SLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 121 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISLSTWMNLVGAAQSGVFAVLTQH 180

Query: 181 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240
           +PGVWSVGLNIDLWCIIYSGIVCSAL VYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 181 RPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 240

Query: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 300
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKE D+FELQNKSQLESDSVHETF QPDLQI AS
Sbjct: 241 VFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKSQLESDSVHETFQQPDLQIGAS 300

Query: 301 PPPNHVLQHQP 312
           PP NHVLQHQP
Sbjct: 301 PPTNHVLQHQP 311

BLAST of Carg10958 vs. ExPASy TrEMBL
Match: A0A6J1EWU5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 311/371 (83.83%), Postives = 311/371 (83.83%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFE----------------------------------------------------- 120
           PLAYFFE                                                     
Sbjct: 61  PLAYFFERKTRPRMTVTLFLEIFVLSLLGMSLSLNMHFASLTYSSPTFVTSSINTIACLT 120

Query: 121 -------RMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 180
                  RMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN
Sbjct: 121 FIIAVMFRMEIVDLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASN 180

Query: 181 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 240
           LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH
Sbjct: 181 LHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQH 240

Query: 241 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 300
           KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF
Sbjct: 241 KPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYF 300

Query: 301 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 312
           VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS
Sbjct: 301 VFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHETFNQPDLQISAS 360

BLAST of Carg10958 vs. ExPASy TrEMBL
Match: A0A6J1KMU7 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111494761 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 300/332 (90.36%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MAMNVRLGVFFTRFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60
           MAMNVRLGVFFTRFKPHIL+IFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF
Sbjct: 1   MAMNVRLGVFFTRFKPHILIIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMF 60

Query: 61  PLAYFFER---------------------MEIVDLKNPRGIAKVVGTLVSLGGVMIMTFY 120
           PLAYFFER                     MEIVDLKNPRGIAKVVGT VSLGGVMIMTFY
Sbjct: 61  PLAYFFERKTRPRMTGTLFLEIFVLSLLGMEIVDLKNPRGIAKVVGTSVSLGGVMIMTFY 120

Query: 121 KGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQLSL 180
           KGPIIRN WHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ+SL
Sbjct: 121 KGPIIRNPWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQISL 180

Query: 181 STWMNLVGAAQSGVFAVLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCTEEKG 240
           STWMNLVGAAQSGVFAVLTQH+PGVWSVGLNIDLWCIIYSGIVCSAL VYIQLWCTEEKG
Sbjct: 181 STWMNLVGAAQSGVFAVLTQHRPGVWSVGLNIDLWCIIYSGIVCSALIVYIQLWCTEEKG 240

Query: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFELQNKS 300
           PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKE D+FELQNKS
Sbjct: 241 PVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKESDEFELQNKS 300

Query: 301 QLESDSVHETFNQPDLQISASPPPNHVLQHQP 312
           QLESDSVHETF QPDLQI ASPP NHVLQHQP
Sbjct: 301 QLESDSVHETFQQPDLQIGASPPTNHVLQHQP 332

BLAST of Carg10958 vs. TAIR 10
Match: AT2G37450.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 108/275 (39.27%), Postives = 166/275 (60.36%), Query Frame = 0

Query: 16  PHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFERMEIVDLK 75
           P IL++  Q+GY  M   T      GM+ +V   YR  VA+V M P A++F+++E V  +
Sbjct: 9   PFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDKLESVKFQ 68

Query: 76  NPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSS 135
           + R  AKVVGT+ ++GG+M+MT  KGP +   W      Q+       + +KG++L    
Sbjct: 69  SIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTKGPSAQNTVGTDIHSSIKGAVLVTIG 128

Query: 136 CISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFA-VLTQHKPGVWSVGLNIDLW 195
           C S+A   I+QA TLK YPA+LSL+TW+ L+G  +  V A V+ +  P VW++G +  L 
Sbjct: 129 CFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLL 188

Query: 196 CIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGG 255
            I YSGIVCSAL  YI     + +GPVFVT F PLC ++VA ++  +F +++Y+G  +G 
Sbjct: 189 TITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGA 248

Query: 256 GIVIMGLYMLLWGKEHDKFELQNKSQLESDSVHET 290
            ++ +GLY+++WGK  D +E  +  Q++ D    T
Sbjct: 249 TVICVGLYLVIWGKAKD-YEYPSTPQIDDDLAQAT 282

BLAST of Carg10958 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 195.7 bits (496), Expect = 5.6e-50
Identity = 114/340 (33.53%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + +P++L+IF Q G    Y    A+   G N +V I YR +VA++ + P A  FE     
Sbjct: 10  KLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRP 69

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  RME V
Sbjct: 70  KMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKV 129

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIRNLW-HPFIHIQHKASNL---HENWLKG 192
           ++   R  AK++GTLV LGG ++MT YKGP+I   W +P +  Q+  +N    H NW+ G
Sbjct: 130 NIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVG 189

Query: 193 SLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSVG 252
           +LL +  C++W+  Y++Q+ T+K YPA LSLS  + L GA QS   A++ +  P  W+VG
Sbjct: 190 TLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVG 249

Query: 253 LNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYM 289
            +  L+  +Y+GIV S +T Y+Q    + +GPVFVT F+PLC +LVA +A F+  ++++ 
Sbjct: 250 WDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHF 309

BLAST of Carg10958 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-47
Identity = 105/325 (32.31%), Postives = 158/325 (48.62%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + KP I +I  Q GY  MY  T  SFKHGMN  +  TYR +VA++ + P A   E     
Sbjct: 8   KLKPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRP 67

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  R+E V
Sbjct: 68  KMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETV 127

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIR--NLWHPFIH---IQHKASNLHENWLK 192
           +LK  R +AKV+GT +++GG M+MT YKGP I      H  +H       +    +NW+ 
Sbjct: 128 NLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVT 187

Query: 193 GSLLTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSV 252
           G+L  + S  +WA  +I+Q+FTLK+YPA+LSL  W+  +G   + + +++       W V
Sbjct: 188 GTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKV 247

Query: 253 GLNIDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLY 273
           G++      +YSG+VCS +  YIQ     E+GPVF T F P+C ++ A L   V  +K++
Sbjct: 248 GMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIH 307

BLAST of Carg10958 vs. TAIR 10
Match: AT2G37450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.4e-47
Identity = 109/314 (34.71%), Postives = 166/314 (52.87%), Query Frame = 0

Query: 16  PHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE-------- 75
           P IL++  Q+GY  M   T      GM+ +V   YR  VA+V M P A++F+        
Sbjct: 9   PFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDNPVIAQNL 68

Query: 76  -------------------------------RMEIVDLKNPRGIAKVVGTLVSLGGVMIM 135
                                          R+E V  ++ R  AKVVGT+ ++GG+M+M
Sbjct: 69  FNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIMVM 128

Query: 136 TFYKGPIIRNLWHPFIHIQHKASNLHENWLKGSLLTVSSCISWALSYIMQAFTLKRYPAQ 195
           T  KGP +   W      Q+       + +KG++L    C S+A   I+QA TLK YPA+
Sbjct: 129 TLVKGPALDLFWTKGPSAQNTVGTDIHSSIKGAVLVTIGCFSYACFMILQAITLKTYPAE 188

Query: 196 LSLSTWMNLVGAAQSGVFA-VLTQHKPGVWSVGLNIDLWCIIYSGIVCSALTVYIQLWCT 255
           LSL+TW+ L+G  +  V A V+ +  P VW++G +  L  I YSGIVCSAL  YI     
Sbjct: 189 LSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGGVVM 248

Query: 256 EEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGSIVGGGIVIMGLYMLLWGKEHDKFEL 290
           + +GPVFVT F PLC ++VA ++  +F +++Y+G  +G  ++ +GLY+++WGK  D +E 
Sbjct: 249 KTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKD-YEY 308

BLAST of Carg10958 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.0e-46
Identity = 102/322 (31.68%), Postives = 160/322 (49.69%), Query Frame = 0

Query: 13  RFKPHILVIFAQVGYTFMYFFTDASFKHGMNPHVHITYRQIVASVGMFPLAYFFE----- 72
           + KP + +I  Q GY  MY  T  SFKHGM+  V  TYR +VA+V M P A  FE     
Sbjct: 8   KIKPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRP 67

Query: 73  -------------------------------------------------------RMEIV 132
                                                                  R+E V
Sbjct: 68  KMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETV 127

Query: 133 DLKNPRGIAKVVGTLVSLGGVMIMTFYKGPIIR--NLWHPFIHIQHKASNLHENWLKGSL 192
           + +    +AKVVGT++++GG MIMT YKGP I      H   H    ++   ++W+ G++
Sbjct: 128 NFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSSTPTGQHWVLGTI 187

Query: 193 LTVSSCISWALSYIMQAFTLKRYPAQLSLSTWMNLVGAAQSGVFAVLTQHKPGVWSVGLN 252
             + S  +WA  +I+Q++TLK YPA+LSL T +  +G   + + +++    P  W +G++
Sbjct: 188 AIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMD 247

Query: 253 IDLWCIIYSGIVCSALTVYIQLWCTEEKGPVFVTMFDPLCTVLVAALAYFVFGQKLYMGS 273
                 +YSG+VCS +  YIQ    +++GPVF T F P+C ++ A L   V  +K+++GS
Sbjct: 248 SGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGS 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022930408.11.5e-177100.00WAT1-related protein At4g08300-like isoform X3 [Cucurbita moschata] >XP_02351400... [more]
XP_022930406.17.6e-17493.67WAT1-related protein At4g08290-like isoform X2 [Cucurbita moschata] >XP_02351400... [more]
XP_023000514.14.6e-17196.46WAT1-related protein At4g08300-like isoform X3 [Cucurbita maxima][more]
XP_022930405.12.5e-16983.83WAT1-related protein At4g08300-like isoform X1 [Cucurbita moschata] >XP_02351400... [more]
XP_023000513.12.4e-16790.36WAT1-related protein At4g08290-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SUF18.0e-4933.53WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
Q501F82.2e-4632.31WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2... [more]
F4IQX14.8e-4634.71WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2... [more]
Q9LPF11.4e-4531.68WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1... [more]
F4HZQ71.3e-4331.44WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1EV317.2e-178100.00WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1[more]
A0A6J1EQV03.7e-17493.67WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1[more]
A0A6J1KK762.2e-17196.46WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111494761 PE=3 SV=1[more]
A0A6J1EWU51.2e-16983.83WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111436862 PE=3 SV=1[more]
A0A6J1KMU71.2e-16790.36WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111494761 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G37450.11.2e-5239.27nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.15.6e-5033.53nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08300.11.5e-4732.31nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37450.23.4e-4734.71nodulin MtN21 /EamA-like transporter family protein [more]
AT1G44800.11.0e-4631.68nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 127..265
e-value: 4.5E-14
score: 52.8
NoneNo IPR availablePANTHERPTHR31218:SF46WAT1-RELATED PROTEINcoord: 14..68
NoneNo IPR availablePANTHERPTHR31218:SF46WAT1-RELATED PROTEINcoord: 68..285
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 174..269
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 14..68
coord: 68..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10958-RACarg10958-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity