Carg10894 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10894
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NADH-cytochrome b5 reductase
Location	Carg_Chr05: 11473530 .. 11478004 (-)
RNA-Seq Expression	Carg10894
Synteny	Carg10894

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCAATTATTCCATTACACATTTCGTACTCTTTCTTTCCTCCGGCGATCATGGCTACGTTCATCCGAAGGCTTGTAAACGCTGCTCCAGCTCTAACGCTCCAATGCATTTGGAGGCCAATCAAAGATCCATTCTTCTAAGTATAGATTCCCTATTGGAGCTATTGCTGCAGTTTCTGGCGGAATCTCGTATATGTATTATGTTTCATCATCGGATTTGGTAATTCAGCTGGATTTTCGTGTTTTCTTATCTTGTTTTTAATTGTATGTGCCGCTTATAAGATCCATTTCGGAGGTTGATTGTATTGAATTGTTACATTCTTATTTATTTTCATAATTTGAAATCGTTGTGATGGATTGGTTTATTACTGAGCTTCATTTTCGTCGTCTGTTCGTATGATTTGGTTGTTGCGGCGTGTTTTATTCTTATGATTATCGAAATTTAATGGACGTCTAGGATGAAGCATCTCGTTGGAGCTTAATGTTCTATTGGTAAATGTTATTCACAGGTTCATCTAGATCAAAACAGTGTAGCGGGAGGCCCAAAAGTTGGTCAGTTTGTCTCATTTGCTATTTCTAGACCAATTTTGTTATAAAAGAAAGAAGAATCTGGCACGAATGAACTTTTAAATTTTTTAGTTTCTTCTTATATTTCATAATCCATGAAAAATGGTTTTGTGCACTTCCATTTCCAGCACTCAAGTCCGATAAATGGATTGAATTCAAGCTGCAAGACACTGCACGGGTTACTCATGATACTCAGTTATTCAGGTACAAAATTTAGGATCCCCGTTTTATGGCACTTTAGTAAGATAATGAAGCATCTATTTGCTTTGAATACTATATAGACATGTCTTTAGTTTTAGATTTCAGGTAAGGAATTGATGTGGGGTTAGCAGAAAACAATTACTAAAACTACTGCCCAAACTTTTCCCTTTGAAATAATGACTGGAGCCACTAGTCTCAATACATTTCGTCGCACTACTATGTATTTTTGTGTTTGCTCTCAGCTCCTTTCTTCTTTCTACACTCCAATATAACATAACCTCACCCATGTAATTTGTGCAGTAATGCAACTAATTATAAGTAGGGACTTTTTATGTATGCAATATTTTCTAATACACAAACTGGGGCAGGTTTTCATTTGATCCTACTGAAAAATTGGGGTTGCATGTCGCTTCATGCCTCCTGACGAGGTAAGTTTTTCTCTTTCCTCCTCCACCCATCTTTTTCAATTTGCTCATGATTGCTAAGTTAAAAGGATTATGCTTCTGGTTATTCATCCCGGTGCGTTGTTGTTACTACAGAGCACCAAGTGGAGTAGATGCTGAGGGAAAAGTAAAATACGTTGTACGCCCGTAAGTCATCTTGCGCAAATATTGGCATGCTTGATTTTGATTTTACCTATATTTGATTATTGAAAATTTGGTATCAGTAAGTTGGAGTTTTTGAGGGTTTAGCATAATGATAAAAAGTCTTTTATAATTCAGCCAATTGATTATGTTTTCAAAATACTTACCTCCTTGGGAAGTTGAGACTTCCAGCCAGTCCATTCAATATTCACAGTTCAATCTTTATGATATTCCAGCTACTTATGCTACCTACTTATTCACCATCAATCCAACCTCTCTTCTTTATATTTACTTGATCTTCATGAAATGATTTTGTTTGTGAGGCTCTTTTTTGAAACATCAAGATGAGACTCGATTGTATTTATTGATGTATGTAATGTGATTCCCTGTATATTTATTCTGTTCAGGTATACACCTATATCAGATCCTGATTCAAAGGGATACTTTGATCTACTGATTAAGGTATGTTGTTCTTCACATTACCGTGTATATTTCCTTAATGTATCTACTTATCCTTTTAAAGACAAAACTGCTATATGATTACTTAATTTGTGGTTTCTTTCGTGAATTTTGCAGGTATATCCTCATGGAAAAATGAGTCAGCATTTTGCTAGCTTGAAACCTGGGGATGTGGTTGAAGTGAAAGGGTATTTTTAATTTATTTTGTCTTCCTCCTTACTTTCAGGCCATTTATTGTATATCGTATATGTCACTCTCTTTTCCCTTAATTGCTTTGCAGCCCCATTGAAAAGATCAGATACTCACCAAATATGAAGAAACACATTGGCATGGTAAGAAGAATCATCTCAGATTGACCGTGAAACTATACGCACACTAACTCAATTGCTGTGCATCCCCATTGAAAGCTCAGATACTCTAGTTTGCATATCATTTTTTGGATTGGCAATCATTTTTAACTTCTAATTTAAAAGATAAAAGTTCCTTTAATTTTTTTATTGACATGCAAGTTATATAGCTTTCTTTGTATCGAGAATGACCATTTCTATTATATGACAGGAAACTGAAAGACGGCTTTAGTGTAATTAGAGTAACTAAATACTGAGATCAACTATACGTGATATCTCAAAATTTTGTTTTCTGCCCATGCAGATTGCTGGTGGCACTGGAATTACTCCCATGCTTCAAGTCATTGATGCTATAGTAAAAAACCCAGATGACAATACCCAGGTGCGCAATCACTTAGTAAAGCAAGTTTCGTTGATTTAAGAAAAGTTCCAATAGAAGACCCTAAACCCTATTTGGAGCTTGTGAAAATGCACCCCAATTGGAACTGTTGTAGTAATTTCCGGTATTTTCTATGGGCAGTGGAGCAGCATTTTCTTCTAACACTTGAGTGATGAACCTGTGAAATTTTGATAATATTACTCTTTGATTTTTCAACCTACCTTCGTTTGTGCAGTATAAACACAAAACTAGCTCAGCTATAGCTTTTTGATGATTGCTTGAGCATGTCCTTTAATGCTTACAGGTCTCGCTGATTTATGGGAATATGTCGCCGGATGATATATTGCTTAAACAGAAACTTGACCTACTTGCATCTAGCCACCCAAATTTAAAGGTATCGTTTATTTGATTAATGAAAAACTGCTGTAATATTTAGAATTGTGTCCTATCAAATGATTGTGAGATGAACGATGACATATTAAGAACCTAGTTTCAGTAGCGTGTTCATGTATGAAGGACAGCCCTTTGTCATCACTAGGGTAATTTCATTTGTATAGAGAGATAAAACAACAGTTTATTGATCGAATGAAATTCCTGCCTCGTAATGAAAGTGATGGACTCTTTGGTAATAAAAATGTAGGTCTTCTACACTGTTGACAATCCATCAAAGAGTTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGCCATTAAAGGCTTACCTGCCCCTGGGGACGACACACTAATCCTCGTAAGTGTTTATTGTTGTTGTTGCTGTAGTACTTCTGTAACCGGTAGTCTTTGCAGTTCTTATCTATTTCATTGCGATATTTCATTTTCCTCTAGCGAGAAGGAAAATGAAAAAATGGTTTAAAAAATCTGTGTTGCCTCTTCCATCTATTGTTCCCATTATTATAATAACATAATGAATCATGCTCTTGTATATCAATTGGGCCGTCTAAATATCTTCATATCATCTAGACCTATATGTGAATTATATCAGTATAAATCGAGTGTGGCCTGTAGAGCCAGGCTCATAGATCCTGTCGTAGCCAATGGTCTGCCTATAAGTTACTTACTAGGACAAACCAATGAAGGCGTTCGAGTCCCTCCACACCCATATGGTTTTGAATGTACTCGTATACACACACCTGGCTACATGGCCAGGGCATTGGTATTGAGGCTATCTAACTTTTACACTAAGAGTCTATCTGTAATTTTTGGTTGAAATGGAAAGAAATTATATGTTTTATAGTTTTACTACCATAGTCATTTCTAGATACATAAACGACTCCCTTCCACGTGTTCAGGTATGCGGTCCACCAGGAATGATGAAACACATATCTGGGGAGAAGGCTAAAGATTACTCACAGGGAGAGGTATAGCAATATATTTGGGCTTTTCACTGTCCTTTCATGAACTTTAATTGATTCATCGACTGTTATTACATATTAATAATCTTTTTTACTGCTTTCTTGTAGCTTGCTGGCATCCTTCAGGAACTTGGATATACTAAGGAAATGGTCTACAAATTCTAACAAGATTGACGTATAAACTTTATGTGAGGAAGTATTTTCTGAAGCATAGGTAGATTTGAATCTTTTGACCAAAAAATTTAAATCAAAATAATTTCCAAGGTGATGTTCATTAGAAAAGTTAATCTTTTGAAGGCCAGTTTATGTTGGCTTTTTCGACTTTTTCTTGAAGTGTATAACACTTGGCTGTTTGTTTCATGTCATGTCGTCCGAGTGAAATAGCATCTCAGCCGGGTCTACCAGGAATGCCTCTGGATTAAGGGGCCGGCATCTCATCCAATTCTAGGGTAATAATAACACGACTGCTTCTCCATAAGTGGTTCGTATCTCGTACTTTTTTTTCAATTGTAGATCTGTTTTTGGATAGACTTTTTTAAGTGCTTAAAAAGTGTCTTGAAGTT

mRNA sequence

CTGCAATTATTCCATTACACATTTCGTACTCTTTCTTTCCTCCGGCGATCATGGCTACGTTCATCCGAAGGCTTGTAAACGCTGCTCCAGCTCTAACGCTCCAATGCATTTGGAGGCCAATCAAAGATCCATTCTTCTAAGTATAGATTCCCTATTGGAGCTATTGCTGCAGTTTCTGGCGGAATCTCGTATATGTATTATGTTTCATCATCGGATTTGGTTCATCTAGATCAAAACAGTGTAGCGGGAGGCCCAAAAGTTGCACTCAAGTCCGATAAATGGATTGAATTCAAGCTGCAAGACACTGCACGGGTTACTCATGATACTCAGTTATTCAGGTTTTCATTTGATCCTACTGAAAAATTGGGGTTGCATGTCGCTTCATGCCTCCTGACGAGAGCACCAAGTGGAGTAGATGCTGAGGGAAAAGTAAAATACGTTGTACGCCCGTATACACCTATATCAGATCCTGATTCAAAGGGATACTTTGATCTACTGATTAAGGTATATCCTCATGGAAAAATGAGTCAGCATTTTGCTAGCTTGAAACCTGGGGATGTGGTTGAAGTGAAAGGCCCCATTGAAAAGATCAGATACTCACCAAATATGAAGAAACACATTGGCATGATTGCTGGTGGCACTGGAATTACTCCCATGCTTCAAGTCATTGATGCTATAGTAAAAAACCCAGATGACAATACCCAGGTCTCGCTGATTTATGGGAATATGTCGCCGGATGATATATTGCTTAAACAGAAACTTGACCTACTTGCATCTAGCCACCCAAATTTAAAGGTCTTCTACACTGTTGACAATCCATCAAAGAGTTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGCCATTAAAGGCTTACCTGCCCCTGGGGACGACACACTAATCCTCGTATGCGGTCCACCAGGAATGATGAAACACATATCTGGGGAGAAGGCTAAAGATTACTCACAGGGAGAGCTTGCTGGCATCCTTCAGGAACTTGGATATACTAAGGAAATGGTCTACAAATTCTAACAAGATTGACGTATAAACTTTATGTGAGGAAGTATTTTCTGAAGCATAGGTAGATTTGAATCTTTTGACCAAAAAATTTAAATCAAAATAATTTCCAAGGTGATGTTCATTAGAAAAGTTAATCTTTTGAAGGCCAGTTTATGTTGGCTTTTTCGACTTTTTCTTGAAGTGTATAACACTTGGCTGTTTGTTTCATGTCATGTCGTCCGAGTGAAATAGCATCTCAGCCGGGTCTACCAGGAATGCCTCTGGATTAAGGGGCCGGCATCTCATCCAATTCTAGGGTAATAATAACACGACTGCTTCTCCATAAGTGGTTCGTATCTCGTACTTTTTTTTCAATTGTAGATCTGTTTTTGGATAGACTTTTTTAAGTGCTTAAAAAGTGTCTTGAAGTT

Coding sequence (CDS)

ATGTATTATGTTTCATCATCGGATTTGGTTCATCTAGATCAAAACAGTGTAGCGGGAGGCCCAAAAGTTGCACTCAAGTCCGATAAATGGATTGAATTCAAGCTGCAAGACACTGCACGGGTTACTCATGATACTCAGTTATTCAGGTTTTCATTTGATCCTACTGAAAAATTGGGGTTGCATGTCGCTTCATGCCTCCTGACGAGAGCACCAAGTGGAGTAGATGCTGAGGGAAAAGTAAAATACGTTGTACGCCCGTATACACCTATATCAGATCCTGATTCAAAGGGATACTTTGATCTACTGATTAAGGTATATCCTCATGGAAAAATGAGTCAGCATTTTGCTAGCTTGAAACCTGGGGATGTGGTTGAAGTGAAAGGCCCCATTGAAAAGATCAGATACTCACCAAATATGAAGAAACACATTGGCATGATTGCTGGTGGCACTGGAATTACTCCCATGCTTCAAGTCATTGATGCTATAGTAAAAAACCCAGATGACAATACCCAGGTCTCGCTGATTTATGGGAATATGTCGCCGGATGATATATTGCTTAAACAGAAACTTGACCTACTTGCATCTAGCCACCCAAATTTAAAGGTCTTCTACACTGTTGACAATCCATCAAAGAGTTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGCCATTAAAGGCTTACCTGCCCCTGGGGACGACACACTAATCCTCGTATGCGGTCCACCAGGAATGATGAAACACATATCTGGGGAGAAGGCTAAAGATTACTCACAGGGAGAGCTTGCTGGCATCCTTCAGGAACTTGGATATACTAAGGAAATGGTCTACAAATTCTAA

Protein sequence

MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLHVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILVCGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF

Homology

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BLAST of Carg10894 vs. NCBI nr
Match: KAG7030398.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 577.8 bits (1488), Expect = 5.1e-161
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP
Sbjct: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS
Sbjct: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV
Sbjct: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF
Sbjct: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 280

[Close]

BLAST of Carg10894 vs. NCBI nr
Match: KAG6599410.1 (NADH-cytochrome b5 reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 576.2 bits (1484), Expect = 1.5e-160
Identity = 279/280 (99.64%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. NCBI nr
Match: XP_022946205.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita moschata] >XP_022946206.1 NADH-cytochrome b5 reductase-like protein [Cucurbita moschata] >XP_022946208.1 NADH-cytochrome b5 reductase-like protein [Cucurbita moschata])

HSP 1 Score: 573.9 bits (1478), Expect = 7.4e-160
Identity = 278/280 (99.29%), Postives = 279/280 (99.64%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQNS AGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQNSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. NCBI nr
Match: XP_023547148.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_023547149.1 NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_023547151.1 NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.5 bits (1469), Expect = 8.2e-159
Identity = 276/280 (98.57%), Postives = 278/280 (99.29%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQNS AGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQNSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYP GKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMA+KGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAVKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. NCBI nr
Match: XP_022999370.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999371.1 NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999372.1 NADH-cytochrome b5 reductase-like protein [Cucurbita maxima])

HSP 1 Score: 564.7 bits (1454), Expect = 4.5e-157
Identity = 273/280 (97.50%), Postives = 276/280 (98.57%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQ S AGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQKSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYP GKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNP KSWKGGKGYV+KDMAIKGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPPKSWKGGKGYVTKDMAIKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGE+AGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGEIAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. ExPASy Swiss-Prot
Match: P83291 (NADH-cytochrome b5 reductase-like protein OS=Arabidopsis thaliana OX=3702 GN=CBR2 PE=1 SV=2)

HSP 1 Score: 477.6 bits (1228), Expect = 9.4e-134
Identity = 220/279 (78.85%), Postives = 254/279 (91.04%), Query Frame = 0


Query: 2   YYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLH 61
           Y  S ++LV+LDQ     GPK AL  DKW+EFKLQDTARV+H+TQLFRFSFDP+ +LGLH
Sbjct: 50  YLTSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAELGLH 109

Query: 62  VASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPG 121
           VASCLLTRAP G +AEGK KYV+RPYTPISDP++KGYFDLLIKVYP GKMSQHFASLKPG
Sbjct: 110 VASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPG 169

Query: 122 DVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSP 181
           DV+EVKGP+EK +YSPNMKKHIGMIAGG+GITPMLQVIDAIVKNP+DNTQ+SL+Y N+SP
Sbjct: 170 DVLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSP 229

Query: 182 DDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILVC 241
           DDILLKQKLD+L ++HPNLK+FYTVDNP+K+WKGG GY+SKDMA+KGLP P DDTLILVC
Sbjct: 230 DDILLKQKLDVLQANHPNLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVC 289

Query: 242 GPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           GPPGMM+HISG KA D+SQGE+ GIL+ELGYT+EMV+KF
Sbjct: 290 GPPGMMEHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of Carg10894 vs. ExPASy Swiss-Prot
Match: A1CRK9 (NADH-cytochrome b5 reductase 2 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=mcr1 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.4e-68
Identity = 135/271 (49.82%), Postives = 176/271 (64.94%), Query Frame = 0


Query: 14  QNSVAGGPKVALKSDK-WIEFKLQDTARVTHDTQLFRFSFDPTEKL-GLHVASCLLTRAP 73
           +  V   PKV    ++ W++ KL +   ++H+T+  RF F   E + GL VAS LLT+  
Sbjct: 57  ETPVVDRPKVFTGGEQGWVDLKLSEIENLSHNTKRLRFEFADKEAVSGLQVASALLTKFK 116

Query: 74  SGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIE 133
               AEGK   V+RPYTP+SD D  GY DL++KVYP+G MS+H  S+     +E KGPI 
Sbjct: 117 ---PAEGKP--VIRPYTPVSDEDQPGYLDLVVKVYPNGPMSEHLHSMNVDQRLEFKGPIP 176

Query: 134 KIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLD 193
           K  +  N  KHI +IAGGTGITPM Q+   I KNP+D T+V+L++GN+S +DILLK++L 
Sbjct: 177 KYPWETNKHKHICLIAGGTGITPMYQLARQIFKNPEDQTKVTLVFGNVSEEDILLKKELQ 236

Query: 194 LLASSHP-NLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAP-GDDTLILVCGPPGMMKH 253
            L ++HP   K FY +DNP K W GGKGYV+K++    LP P  +D  I VCGPPGM K 
Sbjct: 237 ELENTHPRRFKAFYVLDNPPKEWTGGKGYVTKELLKTVLPEPKEEDIKIFVCGPPGMYKA 296

Query: 254 ISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           ISG K     QGEL G+L ELGY K+ VYKF
Sbjct: 297 ISGPKVSPKDQGELTGLLAELGYNKDQVYKF 322

[Close]

BLAST of Carg10894 vs. ExPASy Swiss-Prot
Match: A4QR21 (NADH-cytochrome b5 reductase 2 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=MCR1 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 7.6e-67
Identity = 128/264 (48.48%), Postives = 174/264 (65.91%), Query Frame = 0


Query: 21  PKVALKSDK-WIEFKLQDTARVTHDTQLFRFSFDPTEKL-GLHVASCLLTRAPSGVDAEG 80
           PK AL+ +K ++  +L D   V H+T+  RF     + + GLHVAS LLT+       EG
Sbjct: 72  PKKALEMEKGFVSLQLDDVEVVNHNTKRLRFKLPEDDMVSGLHVASALLTK----FKPEG 131

Query: 81  KVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEKIRYSPN 140
             K V+RPYTPISD D KGY DL++K Y  G MS H   L PG  ++ KGP+ K  +S N
Sbjct: 132 AEKPVLRPYTPISDEDQKGYLDLIVKKYEGGPMSTHIHELVPGQKLDFKGPLPKYEWSAN 191

Query: 141 MKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLDLLASSHP 200
              H+ MIAGGTGITPM Q++ AI KNP D T+V+L+ GN++ +DILLK++L  L +++P
Sbjct: 192 KHPHVAMIAGGTGITPMYQIMRAIFKNPADKTKVTLVVGNITEEDILLKKQLAELENTYP 251

Query: 201 -NLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTL-ILVCGPPGMMKHISGEKAK 260
              + FY +DNP K W G KGY++KD+    LP P ++ + + VCGPPGMMK ISG K  
Sbjct: 252 QRFRAFYVLDNPPKDWAGTKGYITKDLLKTVLPEPKEENIKVFVCGPPGMMKAISGNKVS 311

Query: 261 DYSQGELAGILQELGYTKEMVYKF 281
              QGE++GIL+ELGY ++ +YKF
Sbjct: 312 PKDQGEVSGILKELGYKQDQIYKF 331

[Close]

BLAST of Carg10894 vs. ExPASy Swiss-Prot
Match: Q2HG02 (NADH-cytochrome b5 reductase 2 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=MCR1 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 4.9e-66
Identity = 123/254 (48.43%), Postives = 171/254 (67.32%), Query Frame = 0


Query: 30  WIEFKLQDTARVTHDTQLFRFSFDPTEKL-GLHVASCLLTRAPSGVDAEGKVKYVVRPYT 89
           W+  KL++   V H+++  RF     + + G+HVAS +LT+    VDAE   K V+RPYT
Sbjct: 99  WVSLKLEEVEIVNHNSKRLRFRLPEDDMVSGVHVASAILTKF-KPVDAE---KPVIRPYT 158

Query: 90  PISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEKIRYSPNMKKHIGMIAG 149
           P +D D++GY DLL+K YP+G MS H   + PG  ++VKGP+ K  ++ N   HI ++AG
Sbjct: 159 PTNDEDARGYLDLLVKKYPNGPMSTHLHDMVPGQRLDVKGPLPKYPWTANKHGHIALVAG 218

Query: 150 GTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLDLLASSHP-NLKVFYTVD 209
           GTGITPM Q+  AI  NPDD T+V+L++GN+  DDILLK++L  L +++P   + FY +D
Sbjct: 219 GTGITPMFQLCRAIFNNPDDQTKVTLVFGNVREDDILLKKELAALENNNPRRFRAFYVLD 278

Query: 210 NPSKSWKGGKGYVSKDMAIKGLPAPGDDTL-ILVCGPPGMMKHISGEKAKDYSQGELAGI 269
           +P K W GGKG+++KD+    LP P D+ + + VCGPPGMM  ISG K     QGEL GI
Sbjct: 279 DPPKHWTGGKGFITKDLLKTVLPEPKDENIKVFVCGPPGMMDAISGNKKSPKDQGELKGI 338

Query: 270 LQELGYTKEMVYKF 281
           L+ELGY+ E VYKF
Sbjct: 339 LKELGYSPEQVYKF 348

[Close]

BLAST of Carg10894 vs. ExPASy Swiss-Prot
Match: A2Q898 (NADH-cytochrome b5 reductase 2 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mcr1 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 6.4e-66
Identity = 127/264 (48.11%), Postives = 173/264 (65.53%), Query Frame = 0


Query: 21  PKVALKSDK-WIEFKLQDTARVTHDTQLFRFSFDPTEKL-GLHVASCLLTRAPSGVDAEG 80
           PKV    D+ W + KL +   + H+T+  RF F+  E L GL VAS LLT+         
Sbjct: 64  PKVFKGGDQGWFDLKLSEIEVLNHNTKRLRFEFEDKEALSGLQVASALLTKF-----KPA 123

Query: 81  KVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEKIRYSPN 140
             K V+RPYTP SD ++ GY DL++KVYP+G MS+H  S+  G  ++ KGPI K  +  N
Sbjct: 124 DAKAVIRPYTPTSDEETPGYIDLVVKVYPNGPMSEHLHSMNVGQRLDFKGPIVKYPWETN 183

Query: 141 MKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLDLLASSHP 200
              HI +IAGGTGITPM Q+   I KNP+D T+V+L++GN+  +DILLK++ + L +++P
Sbjct: 184 KHNHICLIAGGTGITPMYQLAREIFKNPEDQTKVTLVFGNVKEEDILLKKEFEELENTYP 243

Query: 201 -NLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTL-ILVCGPPGMMKHISGEKAK 260
              + FY +DNP K W GGKGY+SK++    LP P ++ + I VCGPPGM K ISG K  
Sbjct: 244 RRFRAFYVLDNPPKEWTGGKGYISKELLKTVLPEPKEENIKIFVCGPPGMYKAISGTKNS 303

Query: 261 DYSQGELAGILQELGYTKEMVYKF 281
              QGEL+GIL+ELGY+KE V+KF
Sbjct: 304 PTDQGELSGILKELGYSKEQVFKF 322

[Close]

BLAST of Carg10894 vs. ExPASy TrEMBL
Match: A0A6J1G357 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111450360 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.6e-160
Identity = 278/280 (99.29%), Postives = 279/280 (99.64%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQNS AGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQNSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. ExPASy TrEMBL
Match: A0A6J1KCV4 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111493763 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.2e-157
Identity = 273/280 (97.50%), Postives = 276/280 (98.57%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           MYYVSSSDLVHLDQ S AGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL
Sbjct: 48  MYYVSSSDLVHLDQKSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYP GKMSQHFASLKP
Sbjct: 108 HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLASSHPNLKVFYTVDNP KSWKGGKGYV+KDMAIKGLPAPGDDTLILV
Sbjct: 228 PDDILLKQKLDLLASSHPNLKVFYTVDNPPKSWKGGKGYVTKDMAIKGLPAPGDDTLILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGE+AGILQELGYTKEMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGEIAGILQELGYTKEMVYKF 327

[Close]

BLAST of Carg10894 vs. ExPASy TrEMBL
Match: A0A6J1CNQ7 (NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111013149 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 6.5e-146
Identity = 248/280 (88.57%), Postives = 269/280 (96.07%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           +YYVSS DLVHLDQ++  G PKVAL ++KWIEFKLQDTARVTHDTQLFRFSFDPT+KLGL
Sbjct: 48  LYYVSSPDLVHLDQSNEEGSPKVALNANKWIEFKLQDTARVTHDTQLFRFSFDPTKKLGL 107

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
             ASCLLTRAPSG DAEGK+KYVVRPYTPISD D+KGYFDLLIKVYP GKMSQHFASLKP
Sbjct: 108 DTASCLLTRAPSGQDAEGKIKYVVRPYTPISDSDAKGYFDLLIKVYPQGKMSQHFASLKP 167

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAI+KNPDDNTQVSL+YGN+S
Sbjct: 168 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAILKNPDDNTQVSLLYGNVS 227

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLDLLA+SHPNLK+FYTVDNPSK+WKGG+GY+SKDMA+KGLPAPG+DT ILV
Sbjct: 228 PDDILLKQKLDLLAASHPNLKIFYTVDNPSKNWKGGQGYISKDMAVKGLPAPGNDTQILV 287

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISGEKAKDYSQGEL+GIL+ELGYT+EMVYKF
Sbjct: 288 CGPPGMMKHISGEKAKDYSQGELSGILKELGYTEEMVYKF 327

[Close]

BLAST of Carg10894 vs. ExPASy TrEMBL
Match: A0A5A7V0C8 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00120 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 241/280 (86.07%), Postives = 262/280 (93.57%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           +YYVSSSD+VHLD NS     KVALKS KWIEFKLQD+ARVTH+TQLFRFSFDP+E LGL
Sbjct: 50  LYYVSSSDMVHLDPNSEEASSKVALKSGKWIEFKLQDSARVTHNTQLFRFSFDPSETLGL 109

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           +VASC+ TRAP G D EGKVKYVVRPYTPISDPD++GYFDLLIKVYP GKMSQHFASLKP
Sbjct: 110 NVASCIYTRAPIGQDDEGKVKYVVRPYTPISDPDARGYFDLLIKVYPQGKMSQHFASLKP 169

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDV+EVKGPIEK++YSPNMKK IGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGN+S
Sbjct: 170 GDVLEVKGPIEKLKYSPNMKKRIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVS 229

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLD+LASSHPNLKVFYTVDNPS++WKGGKGYVSKDM IKGLP PGDD+LILV
Sbjct: 230 PDDILLKQKLDILASSHPNLKVFYTVDNPSQNWKGGKGYVSKDMVIKGLPPPGDDSLILV 289

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISG+KA DYSQGEL G+L+ELGYTKEMVYKF
Sbjct: 290 CGPPGMMKHISGDKAPDYSQGELDGMLKELGYTKEMVYKF 329

[Close]

BLAST of Carg10894 vs. ExPASy TrEMBL
Match: A0A1S3C7J4 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103497367 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 241/280 (86.07%), Postives = 262/280 (93.57%), Query Frame = 0


Query: 1   MYYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGL 60
           +YYVSSSD+VHLD NS     KVALKS KWIEFKLQD+ARVTH+TQLFRFSFDP+E LGL
Sbjct: 50  LYYVSSSDMVHLDPNSEEASSKVALKSGKWIEFKLQDSARVTHNTQLFRFSFDPSETLGL 109

Query: 61  HVASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKP 120
           +VASC+ TRAP G D EGKVKYVVRPYTPISDPD++GYFDLLIKVYP GKMSQHFASLKP
Sbjct: 110 NVASCIYTRAPIGQDDEGKVKYVVRPYTPISDPDARGYFDLLIKVYPQGKMSQHFASLKP 169

Query: 121 GDVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMS 180
           GDV+EVKGPIEK++YSPNMKK IGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGN+S
Sbjct: 170 GDVLEVKGPIEKLKYSPNMKKRIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVS 229

Query: 181 PDDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILV 240
           PDDILLKQKLD+LASSHPNLKVFYTVDNPS++WKGGKGYVSKDM IKGLP PGDD+LILV
Sbjct: 230 PDDILLKQKLDILASSHPNLKVFYTVDNPSQNWKGGKGYVSKDMVIKGLPPPGDDSLILV 289

Query: 241 CGPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           CGPPGMMKHISG+KA DYSQGEL G+L+ELGYTKEMVYKF
Sbjct: 290 CGPPGMMKHISGDKAPDYSQGELDGMLKELGYTKEMVYKF 329

[Close]

BLAST of Carg10894 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 477.6 bits (1228), Expect = 6.7e-135
Identity = 220/279 (78.85%), Postives = 254/279 (91.04%), Query Frame = 0


Query: 2   YYVSSSDLVHLDQNSVAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLH 61
           Y  S ++LV+LDQ     GPK AL  DKW+EFKLQDTARV+H+TQLFRFSFDP+ +LGLH
Sbjct: 50  YLTSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAELGLH 109

Query: 62  VASCLLTRAPSGVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPG 121
           VASCLLTRAP G +AEGK KYV+RPYTPISDP++KGYFDLLIKVYP GKMSQHFASLKPG
Sbjct: 110 VASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPG 169

Query: 122 DVVEVKGPIEKIRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSP 181
           DV+EVKGP+EK +YSPNMKKHIGMIAGG+GITPMLQVIDAIVKNP+DNTQ+SL+Y N+SP
Sbjct: 170 DVLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSP 229

Query: 182 DDILLKQKLDLLASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILVC 241
           DDILLKQKLD+L ++HPNLK+FYTVDNP+K+WKGG GY+SKDMA+KGLP P DDTLILVC
Sbjct: 230 DDILLKQKLDVLQANHPNLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVC 289

Query: 242 GPPGMMKHISGEKAKDYSQGELAGILQELGYTKEMVYKF 281
           GPPGMM+HISG KA D+SQGE+ GIL+ELGYT+EMV+KF
Sbjct: 290 GPPGMMEHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of Carg10894 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 195.3 bits (495), Expect = 6.6e-50
Identity = 106/261 (40.61%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 22  KVALKSDKWIEFKLQDTARVTHDTQLFRFSF-DPTEKLGLHVASCLLTRAPSGVDAEGKV 81
           +V L  + + EFKL    +++H+   F F     T  LGL +   +  R   G D +G  
Sbjct: 39  RVCLDPENFKEFKLVKRHQLSHNVAKFVFELPTSTSVLGLPIGQHISCR---GKDGQG-- 98

Query: 82  KYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEKIRYSPNMK 141
           + V++PYTP +     G F+L+IK+YP G+MS HF  ++ GD + VKGP  + +Y P   
Sbjct: 99  EDVIKPYTPTTLDSDVGRFELVIKMYPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQF 158

Query: 142 KHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKLDLLASSHP-N 201
           +  GM+AGG+GITPM QV  AI++NP D T+V LIY N++ DDILLK++L+ L +++P  
Sbjct: 159 RAFGMLAGGSGITPMFQVARAILENPTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQ 218

Query: 202 LKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILVCGPPGMMKHISGEKAKDYS 261
            K+FY ++ P + W GG G+VSK+M     PAP  D  IL CGPP M K           
Sbjct: 219 FKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAPASDIQILRCGPPPMNK----------- 278

Query: 262 QGELAGILQELGYTKEMVYKF 281
              +A  L+ LGY+ EM ++F
Sbjct: 279 --AMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Carg10894 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 141.0 bits (354), Expect = 1.5e-33
Identity = 83/245 (33.88%), Postives = 133/245 (54.29%), Query Frame = 0


Query: 31  IEFKLQDTARVTHDTQLFRFSFDPTEKL------GLHVASCLLTRAPSGVDAEGKVKYVV 90
           +  +L +   ++HD + FRF+  P E +      G H+  C          A    K  +
Sbjct: 663 VPVQLVEKTSISHDVRKFRFAL-PVEDMVLGLPVGKHIFLC----------ATINDKLCL 722

Query: 91  RPYTPISDPDSKGYFDLLIKVY--------PH-GKMSQHFASLKPGDVVEVKGPIEKIRY 150
           R YTP S  D  GYF+L++K+Y        P+ G MSQ+  SL  G  +E+KGP+  + Y
Sbjct: 723 RAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEY 782

Query: 151 ----------SPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDIL 210
                      P     + M+AGGTGITP+ Q+I AI+K+P+D T++ +IY N + +DIL
Sbjct: 783 LGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDIL 842

Query: 211 LKQKLDLLASSHPN-LKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGD-DTLILVCGP 249
           L+++LD  A  +P+ LKV+Y V++  + W    G++S+ +  + +P   D   L + CGP
Sbjct: 843 LREELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGP 896

[Close]

BLAST of Carg10894 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 140.6 bits (353), Expect = 1.9e-33
Identity = 81/240 (33.75%), Postives = 135/240 (56.25%), Query Frame = 0


Query: 31  IEFKLQDTARVTHDTQLFRFSF-DPTEKLGLHVASCLLTRAPSGVDAEGKVKYVVRPYTP 90
           I  +L +   ++HD + FRF+     ++LGL V   +       V A    K  +R YTP
Sbjct: 663 IPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVF------VCANINDKLCLRAYTP 722

Query: 91  ISDPDSKGYFDLLIKVY--------PH-GKMSQHFASLKPGDVVEVKGPIEKIRY----- 150
            S  D+ G+ DL++KVY        P+ G MSQH  SL  G ++++KGP+  I Y     
Sbjct: 723 TSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGN 782

Query: 151 -----SPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNMSPDDILLKQKL 210
                 P   K + M+AGGTGITP+ Q+I +I+ +P+D T++ ++Y N + DDIL++++L
Sbjct: 783 FLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREEL 842

Query: 211 DLLASSH-PNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGD-DTLILVCGPPGMMK 249
           +  AS H   LK++Y V+   + W    G++++ +  + +P   + ++L L CGPP M++
Sbjct: 843 EGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQ 896

The following BLAST results are available for this feature:

BLAST of Carg10894 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030398.1	5.1e-161	100.00	NADH-cytochrome b5 reductase-like protein [Cucurbita argyrosperma subsp. argyros...	[more]
KAG6599410.1	1.5e-160	99.64	NADH-cytochrome b5 reductase-like protein, partial [Cucurbita argyrosperma subsp...	[more]
XP_022946205.1	7.4e-160	99.29	NADH-cytochrome b5 reductase-like protein [Cucurbita moschata] >XP_022946206.1 N...	[more]
XP_023547148.1	8.2e-159	98.57	NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_02354...	[more]
XP_022999370.1	4.5e-157	97.50	NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999371.1 NAD...	[more]

BLAST of Carg10894 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83291	9.4e-134	78.85	NADH-cytochrome b5 reductase-like protein OS=Arabidopsis thaliana OX=3702 GN=CBR...	[more]
A1CRK9	1.4e-68	49.82	NADH-cytochrome b5 reductase 2 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 5...	[more]
A4QR21	7.6e-67	48.48	NADH-cytochrome b5 reductase 2 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-46...	[more]
Q2HG02	4.9e-66	48.43	NADH-cytochrome b5 reductase 2 OS=Chaetomium globosum (strain ATCC 6205 / CBS 14...	[more]
A2Q898	6.4e-66	48.11	NADH-cytochrome b5 reductase 2 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1...	[more]

BLAST of Carg10894 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G357	3.6e-160	99.29	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111450360 PE=3 ...	[more]
A0A6J1KCV4	2.2e-157	97.50	NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111493763 PE=3 SV...	[more]
A0A6J1CNQ7	6.5e-146	88.57	NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111013149 PE=3...	[more]
A0A5A7V0C8	1.8e-140	86.07	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3C7J4	1.8e-140	86.07	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103497367 PE=3 SV=1	[more]

BLAST of Carg10894 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20080.1	6.7e-135	78.85	FAD/NAD(P)-binding oxidoreductase	[more]
AT5G17770.1	6.6e-50	40.61	NADH:cytochrome B5 reductase 1	[more]
AT1G37130.1	1.5e-33	33.88	nitrate reductase 2	[more]
AT1G77760.1	1.9e-33	33.75	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 144..163 score: 40.46 coord: 237..245 score: 44.57 coord: 182..193 score: 30.65 coord: 85..92 score: 44.83 coord: 170..179 score: 25.29
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 85..92 score: 66.93 coord: 121..135 score: 45.69 coord: 182..193 score: 54.51 coord: 144..163 score: 64.69 coord: 237..245 score: 63.43
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 33..135 e-value: 1.7E-24 score: 86.0
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 23..126 e-value: 4.2E-35 score: 122.4
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 23..280
None	No IPR available	PANTHER	PTHR19370:SF171	NADH-CYTOCHROME B5 REDUCTASE-LIKE	coord: 23..280
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 33..280 e-value: 4.93614E-111 score: 318.357
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 145..251 e-value: 3.9E-32 score: 111.2
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 127..280 e-value: 3.0E-43 score: 149.0
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 122..279
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 28..136 score: 13.973775
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 22..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10894-RA	Carg10894-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity