Carg10814 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10814
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	60S ribosomal protein L28-2-like
Location	Carg_Chr05: 11961516 .. 11964617 (-)
RNA-Seq Expression	Carg10814
Synteny	Carg10814

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTTCTGTTTCTGCATCTCTCTACCACTTGTTGCAGCGCAGGTCCGTTCGTTCTTCTTGATCTTATTTTCTCTTTTGTTTTCATCGTCTTCAATCAGATTTTGATCCATTTTTCTGATTTCAATTGTGTATGTCTCATTTCAACTGGCATACTTACATCTGTAGCCGATCTCCGTTAGCAATCTTCGGAATATATTTGTTTCTTGTTCATCGGATTAGTTGTTCCGATACTTATTATTGTTATCATTTTTAAATCTTATGTAGATGGCGAACGTTCCGGGTCAGTTAGTCTGGGAAATCGTTAAGAAGAACAGTTGTTTCCTCGTAAAAGAGTTTGGCAGAGGCAGTGCTGGTGTTCAATTCAGCAAAGAGCCTAACAACCTCTACAACCTCAACTCCTACAAGCATTCCGGTAAGATTCGAATCAGTCATCTGTTTCCATTTTTACTTCTTTAGTACCATCGTTTAAATATGAGGAAGATATTTTTTCCTCTACATTCATGATCTGTCTTCATCTCGTTGACCCCTTTAACCAGTTTTACCTCAGAGACGATTGGCATTTTGGGTTTTGTTCTAATGTGTTGGGTTTAATACTAATTTGTTTATGGAACTTTCTTTGGGTGCTCTTTCAGGGTTGGCCAACAAGAAGACGGTGACTATTCAGCCAGGAGGCAAAGATTTGTCTGTATTGCTTGCGACGACAAAGAGAAAGAAGCAGAATAAACCCGCGAGTTTGCTCAACAAATCTGTTATGAGGAAGGAATTTTCAAGTATGGCCAAGGCTGTGACTAATCAGGTAACTTGCCTCTCCTTCAATTTTCATTCATGTCCATTGTGATGAAATTTGTGATTCCCAAGTCAGCTCATACCGGTCTTTTGTCAGTTACATATTGTATATAGATTTCCCGTTGAATCTTTTTGTATAAGATCTTACCTGCCCGTGTCTTCAATGAGCAGTCAATGAATCTTTTCTATTTATAGTTTTCTTGGATTTCGTGATTGATTTGAATGTGAAACTGAATGTGCATTGTTGCAGTGATGAAAAGCTAACATGTAAGAAGGGCCACTTTCGTAGTGGCATCTTTGATGAAATCTATTTGTTACGCTTGGCTTCTGAGCATAATTGTCCATTTGCAACCATGTTAAACTAAATCATGTTAAACAATTTAGGTCGAATATTATTGTTTAGGTACTCCCTTTCCATCACTTTTATGAGAAAACTGAACGACCCAAAATTTGACGGTGTTGTACTTGTGGGCAACCGTAAAACTGTTAATGCGATGATGACCTCTTACATGTTTGGAGGTCACCTTTATAGTGGCATCTTCGATGAAATCTTTTTGTAGTGCATGGCTTCATTTCATTTTCCTGTTACTTCCTCGTGTAGCATAATATCAAACTTCTGTTAACATTATACTTCAACAGAAATTTAAAGGAACAACTAAGGGTGGGCACACATATTAACTGTTACTCATGCTGCAATGATGAGCTGTTAACATGTAAGAAGGGTCACTTTCGTAGTGGCATCTTCGATGCAATCTTTTTGTTACGCTTGGCTTCTGAGCAACAATTGTTCTTTTAGTTGAAAACAACTTTGAACCACATAAATTTGCCTTACAAGTTATTGAACAACTTTTAACTGTTGCTGCTATGATGAGCTGCTAACATGTAAGAAGGGTCACTTTCGTAGTGGCATCTTCGATGCAATCTTTTTGTTACGCTTGGCTTCTGAGCGACAATTCTCCTCTTATAGATGAAAGCAACTTCAATCTCTTAAATTTGCTTTGTTTGTTTGGATCTCTGGTTATGTTCCTCAACACTTATATTCATGTAATTTATAATAACTTCCTCATGTAGCATAATATCCAACTTGCTAATATGTAAACCCTAGGGGCGGACAAAAGTTAACTCTTACTGCTATGATGAGCTGCTAACATGTAAGAAGGGGTCACTTTTGTAGTGGCATCTTCAGTGCAATCTTTTTGTTACGCTTGGCTTCTGAGCATAAACTCTCCTCTAGGATGAAAACAATTTCGATCTTTTGTATTAGCTTTGCTTTGCCTGATTGGGTCTCTGATTATATTCCCCAACTTTTTTTTCATGTTATTTCCCGTATCTTCCCCTTAGCATAATATCATACTAAAGCTAATATTAACTGTGTGATGATGAGCTGCTAACATGTAAGAAGCGGTCACTTTCGTAGTGGCATCTTCAATGCAATTCTTTTGTTACGCTTGGCTTCTGAGCATTAATTTACTCTTGGATGTAAACAACTTGGAGCTTTTATTGTGCTTTGCTTGATTGGATCTCTGATAACTTCCTCATGTAGCATTATTTAAGCTTGAGCTGATATTATTCTCTACACCAGAAAGTTAAAACTGTTACTGCGATGATGAGCTGCTAACATGTAAGAAGCGGCCACTTTCGTAGTGGCATCTTCAATGAAATCTTTTTGTTACGCTTGGCTTGTGAGCATTAATTTGTTCTGTTGTACTACATCTGTGTATTGTTTTGTTTATATCAAAAGTAATTTGGAGTGTACTTGTTGATTTTCAGGTGGCTGACAATTACTACCGACCGGACTTGAAGAAGGCTGCCCTTGCAAAGCTAAGTGCAGTTCACAGGAGCCTCAAAATTGGCAAGTCAGGTGTGAAGAAGAGGAACAGGCAAGCTGTTAGGAGCCGCGGTAGGAAGTGAAACAACAATGGTTTATAAGGATTTTCTTTTCTTCCATTCGAGCCCCCCTGTTTTTTACCCTGTTTTTTACCCTGTTTCCCATGTTCTAAAGCGACCCATTTGCAGGGCTTTTCATTATACTTTTTATGTACCCTACTTGAAAAATTAGGATTTTTGACTATTTTGGTTTATATCTGAGCCATCCTGCCATTTGGATTTTTGTTCCTTCAAGAGATTATACTAAATTTTAGCTTTAGAGCTACTATGCATTGTCTGTTCTTGGTCTTAAAATTCCTTTTTAATCATTTAGAAATCAAATCAATAGTTCAATGAATTAAAAGCTCTTCATATTAAAATCTATTAGGGTTGATGAATAGCTTGTTACAATTAATTTTACAGTATAATTATTTCCACAAGGGTTATATA

mRNA sequence

CTCTTCTGTTTCTGCATCTCTCTACCACTTGTTGCAGCGCAGGTCCGTTCGTTCTTCTTGATCTTATTTTCTCTTTTGTTTTCATCGTCTTCAATCAGATTTTGATCCATTTTTCTGATTTCAATTGTGTATGTCTCATTTCAACTGGCATACTTACATCTGTAGCCGATCTCCGTTAGCAATCTTCGGAATATATTTGTTTCTTGTTCATCGGATTAGTTGTTCCGATACTTATTATTGTTATCATTTTTAAATCTTATGTAGATGGCGAACGTTCCGGGTCAGTTAGTCTGGGAAATCGTTAAGAAGAACAGTTGTTTCCTCGTAAAAGAGTTTGGCAGAGGCAGTGCTGGTGTTCAATTCAGCAAAGAGCCTAACAACCTCTACAACCTCAACTCCTACAAGCATTCCGGGTTGGCCAACAAGAAGACGGTGACTATTCAGCCAGGAGGCAAAGATTTGTCTGTATTGCTTGCGACGACAAAGAGAAAGAAGCAGAATAAACCCGCGAGTTTGCTCAACAAATCTGTTATGAGGAAGGAATTTTCAAGTATGGCCAAGGCTGTGACTAATCAGGTGGCTGACAATTACTACCGACCGGACTTGAAGAAGGCTGCCCTTGCAAAGCTAAGTGCAGTTCACAGGAGCCTCAAAATTGGCAAGTCAGGTGTGAAGAAGAGGAACAGGCAAGCTGTTAGGAGCCGCGGTAGGAAGTGAAACAACAATGGTTTATAAGGATTTTCTTTTCTTCCATTCGAGCCCCCCTGTTTTTTACCCTGTTTTTTACCCTGTTTCCCATGTTCTAAAGCGACCCATTTGCAGGGCTTTTCATTATACTTTTTATGTACCCTACTTGAAAAATTAGGATTTTTGACTATTTTGGTTTATATCTGAGCCATCCTGCCATTTGGATTTTTGTTCCTTCAAGAGATTATACTAAATTTTAGCTTTAGAGCTACTATGCATTGTCTGTTCTTGGTCTTAAAATTCCTTTTTAATCATTTAGAAATCAAATCAATAGTTCAATGAATTAAAAGCTCTTCATATTAAAATCTATTAGGGTTGATGAATAGCTTGTTACAATTAATTTTACAGTATAATTATTTCCACAAGGGTTATATA

Coding sequence (CDS)

ATGGCGAACGTTCCGGGTCAGTTAGTCTGGGAAATCGTTAAGAAGAACAGTTGTTTCCTCGTAAAAGAGTTTGGCAGAGGCAGTGCTGGTGTTCAATTCAGCAAAGAGCCTAACAACCTCTACAACCTCAACTCCTACAAGCATTCCGGGTTGGCCAACAAGAAGACGGTGACTATTCAGCCAGGAGGCAAAGATTTGTCTGTATTGCTTGCGACGACAAAGAGAAAGAAGCAGAATAAACCCGCGAGTTTGCTCAACAAATCTGTTATGAGGAAGGAATTTTCAAGTATGGCCAAGGCTGTGACTAATCAGGTGGCTGACAATTACTACCGACCGGACTTGAAGAAGGCTGCCCTTGCAAAGCTAAGTGCAGTTCACAGGAGCCTCAAAATTGGCAAGTCAGGTGTGAAGAAGAGGAACAGGCAAGCTGTTAGGAGCCGCGGTAGGAAGTGA

Protein sequence

MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQPGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALAKLSAVHRSLKIGKSGVKKRNRQAVRSRGRK

Homology

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BLAST of Carg10814 vs. NCBI nr
Match: XP_022946829.1 (60S ribosomal protein L28-2-like [Cucurbita moschata] >KAG7030478.1 60S ribosomal protein L28-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.7 bits (735), Expect = 5.7e-74
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK
Sbjct: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 150

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BLAST of Carg10814 vs. NCBI nr
Match: KAG6599502.1 (60S ribosomal protein L28-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 284.3 bits (726), Expect = 6.3e-73
Identity = 148/150 (98.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWE+VKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 151 MANVPGQLVWEMVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 210

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA
Sbjct: 211 PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 270

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKI KSGVKKRNRQAVRSRGRK
Sbjct: 271 KLSAVHRSLKIAKSGVKKRNRQAVRSRGRK 300

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BLAST of Carg10814 vs. NCBI nr
Match: XP_022999539.1 (60S ribosomal protein L28-1-like [Cucurbita maxima] >XP_022999540.1 60S ribosomal protein L28-1-like [Cucurbita maxima])

HSP 1 Score: 282.3 bits (721), Expect = 2.4e-72
Identity = 147/150 (98.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEF SMAKAVTNQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFPSMAKAVTNQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKI KSGVKKRNRQAVR+RGRK
Sbjct: 121 KLSAVHRSLKIAKSGVKKRNRQAVRTRGRK 150

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BLAST of Carg10814 vs. NCBI nr
Match: XP_023545529.1 (60S ribosomal protein L28-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.9 bits (712), Expect = 2.6e-71
Identity = 145/150 (96.67%), Postives = 146/150 (97.33%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNSCFLVKEFG GSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSCFLVKEFGNGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEF SM KAVTNQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFPSMVKAVTNQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKI KSGVKKRNRQAVR+RGRK
Sbjct: 121 KLSAVHRSLKIAKSGVKKRNRQAVRTRGRK 150

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BLAST of Carg10814 vs. NCBI nr
Match: XP_038889445.1 (60S ribosomal protein L28-1-like [Benincasa hispida])

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-66
Identity = 135/150 (90.00%), Postives = 142/150 (94.67%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNS FL KEFGRG+A +QFSKEPNNLYNLNSYKHSGLAN+KTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSSFLFKEFGRGNASIQFSKEPNNLYNLNSYKHSGLANQKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTK KKQNKPASLL+KS+MRKEF  MAKAVTNQVADNYYRPDLK AALA
Sbjct: 61  PGGKDLSVLLATTKTKKQNKPASLLHKSIMRKEFPRMAKAVTNQVADNYYRPDLKNAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLK+ KSGVKKRNRQAVR+RGRK
Sbjct: 121 KLSAVHRSLKVAKSGVKKRNRQAVRTRGRK 150

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BLAST of Carg10814 vs. ExPASy Swiss-Prot
Match: Q9M0E2 (60S ribosomal protein L28-2 OS=Arabidopsis thaliana OX=3702 GN=RPL28C PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.2e-53
Identity = 103/143 (72.03%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK+N+CFLVK+FGRG+A VQFSKE NNL N+NSYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKRNNCFLVKQFGRGNAKVQFSKESNNLVNINSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
             GKD  V+L TTK K+QNKP   +NKS+++KEFS M+K V NQV DNYYRPDLKKAALA
Sbjct: 61  AAGKDQGVVLGTTKTKRQNKPKLSVNKSILKKEFSRMSKVVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGPKRRNRQA 143

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BLAST of Carg10814 vs. ExPASy Swiss-Prot
Match: O82204 (60S ribosomal protein L28-1 OS=Arabidopsis thaliana OX=3702 GN=RPL28A PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.2e-52
Identity = 102/143 (71.33%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK N+CFLVK+FGRG++ VQFSKE NNL N++SYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKNNNCFLVKQFGRGNSKVQFSKETNNLTNVHSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
              KD +V+LATTK KKQNKP   +NKS+++KEF  M+KAV NQV DNYYRPDLKKAALA
Sbjct: 61  AADKDQAVVLATTKTKKQNKPKLSVNKSILKKEFPRMSKAVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGAKQRNRQA 143

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BLAST of Carg10814 vs. ExPASy Swiss-Prot
Match: Q3T0L7 (60S ribosomal protein L28 OS=Bos taurus OX=9913 GN=RPL28 PE=2 SV=3)

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-12
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 8   LVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQPGGKDLS 67
           L W +V+  S FL+K   +      +S EPNNL   NS++++GL ++KTV ++P      
Sbjct: 5   LQWMVVRNCSSFLIKRNKQ-----TYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKG 64

Query: 68  VLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALAKLSAVHR 127
           V++   +R  Q KPA+   ++ + K   +   ++ + +  N YRPDL+ AA+ + SA+ R
Sbjct: 65  VVVVMKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILR 122

Query: 128 SLK 131
           S K
Sbjct: 125 SQK 122

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BLAST of Carg10814 vs. ExPASy Swiss-Prot
Match: P46779 (60S ribosomal protein L28 OS=Homo sapiens OX=9606 GN=RPL28 PE=1 SV=3)

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-12
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 8   LVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQPGGKDLS 67
           L W +V+  S FL+K   +      +S EPNNL   NS++++GL ++KTV ++P      
Sbjct: 5   LQWMVVRNCSSFLIKRNKQ-----TYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKG 64

Query: 68  VLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALAKLSAVHR 127
           V++   +R  Q KPA+   ++ + K   +   ++ + +  N YRPDL+ AA+ + SA+ R
Sbjct: 65  VVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILR 122

Query: 128 SLK 131
           S K
Sbjct: 125 SQK 122

[Close]

BLAST of Carg10814 vs. ExPASy Swiss-Prot
Match: P41105 (60S ribosomal protein L28 OS=Mus musculus OX=10090 GN=Rpl28 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-12
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 8   LVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQPGGKDLS 67
           L W +V+  S FL+K   +      +S EPNNL   NS++++GL ++KTV ++P      
Sbjct: 5   LQWMVVRNCSSFLIKRNKQ-----TYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKG 64

Query: 68  VLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALAKLSAVHR 127
           V++   +R  Q KPA+   ++ + K   +   ++ + +  N YRPDL+ AA+ + SA+ R
Sbjct: 65  VVVVMKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILR 122

Query: 128 SLK 131
           S K
Sbjct: 125 SQK 122

[Close]

BLAST of Carg10814 vs. ExPASy TrEMBL
Match: A0A6J1G510 (60S ribosomal protein L28-2-like OS=Cucurbita moschata OX=3662 GN=LOC111450772 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.7e-74
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK
Sbjct: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 150

[Close]

BLAST of Carg10814 vs. ExPASy TrEMBL
Match: A0A6J1KHD5 (60S ribosomal protein L28-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493869 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.2e-72
Identity = 147/150 (98.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEF SMAKAVTNQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFPSMAKAVTNQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLKI KSGVKKRNRQAVR+RGRK
Sbjct: 121 KLSAVHRSLKIAKSGVKKRNRQAVRTRGRK 150

[Close]

BLAST of Carg10814 vs. ExPASy TrEMBL
Match: A0A6J1DMI9 (60S ribosomal protein L28-1-like OS=Momordica charantia OX=3673 GN=LOC111021927 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.7e-66
Identity = 134/150 (89.33%), Postives = 143/150 (95.33%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKN+ FLVKEFGRG+AGVQFSKEPNNLYNLNSYKHSGLAN+KTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNNSFLVKEFGRGNAGVQFSKEPNNLYNLNSYKHSGLANRKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLATTK KKQNKPASLL++SVMRKEF  MAKAV NQVADNYYRPDLKKAALA
Sbjct: 61  PGGKDLSVLLATTKAKKQNKPASLLHRSVMRKEFPRMAKAVINQVADNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLS VHRSLK+ KSGVKKRNRQA+++RGRK
Sbjct: 121 KLSVVHRSLKVAKSGVKKRNRQALKTRGRK 150

[Close]

BLAST of Carg10814 vs. ExPASy TrEMBL
Match: A0A6J1FD25 (60S ribosomal protein L28-2-like OS=Cucurbita moschata OX=3662 GN=LOC111444289 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.0e-64
Identity = 129/150 (86.00%), Postives = 140/150 (93.33%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNS FLVKEFGRG+AGVQFSKEPNNLYNLNSYKHSGLAN+KTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSSFLVKEFGRGNAGVQFSKEPNNLYNLNSYKHSGLANRKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
            GGKDLS+L+ATTK KKQNKPASLL+KS+ RKEF  M K VTNQVADNYYRPDLK AALA
Sbjct: 61  AGGKDLSILIATTKTKKQNKPASLLHKSITRKEFPRMVKTVTNQVADNYYRPDLKNAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLK+ +SGVKKRNRQA+++RGRK
Sbjct: 121 KLSAVHRSLKVARSGVKKRNRQAIKTRGRK 150

[Close]

BLAST of Carg10814 vs. ExPASy TrEMBL
Match: A0A1S3CAX2 (60S ribosomal protein L28-1-like OS=Cucumis melo OX=3656 GN=LOC103498417 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.4e-64
Identity = 133/150 (88.67%), Postives = 140/150 (93.33%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MANVPGQLVWEIVKKNS FLVKEFGRG+A VQFSKEPNNLY+LNSYKHSGLAN KTVTIQ
Sbjct: 1   MANVPGQLVWEIVKKNSSFLVKEFGRGNASVQFSKEPNNLYSLNSYKHSGLANLKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
           PGGKDLSVLLAT+K KKQNKPA+ L+KSVMRKEF  MAKAVTNQVADNYYRPDLK AALA
Sbjct: 61  PGGKDLSVLLATSKTKKQNKPANSLHKSVMRKEFPRMAKAVTNQVADNYYRPDLKNAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQAVRSRGRK 151
           KLSAVHRSLK+ KSGVKK NRQAVR+RGRK
Sbjct: 121 KLSAVHRSLKVAKSGVKKGNRQAVRTRGRK 150

[Close]

BLAST of Carg10814 vs. TAIR 10
Match: AT4G29410.1 (Ribosomal L28e protein family )

HSP 1 Score: 210.7 bits (535), Expect = 8.2e-55
Identity = 103/143 (72.03%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK+N+CFLVK+FGRG+A VQFSKE NNL N+NSYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKRNNCFLVKQFGRGNAKVQFSKESNNLVNINSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
             GKD  V+L TTK K+QNKP   +NKS+++KEFS M+K V NQV DNYYRPDLKKAALA
Sbjct: 61  AAGKDQGVVLGTTKTKRQNKPKLSVNKSILKKEFSRMSKVVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGPKRRNRQA 143

[Close]

BLAST of Carg10814 vs. TAIR 10
Match: AT4G29410.2 (Ribosomal L28e protein family )

HSP 1 Score: 210.7 bits (535), Expect = 8.2e-55
Identity = 103/143 (72.03%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK+N+CFLVK+FGRG+A VQFSKE NNL N+NSYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKRNNCFLVKQFGRGNAKVQFSKESNNLVNINSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
             GKD  V+L TTK K+QNKP   +NKS+++KEFS M+K V NQV DNYYRPDLKKAALA
Sbjct: 61  AAGKDQGVVLGTTKTKRQNKPKLSVNKSILKKEFSRMSKVVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGPKRRNRQA 143

[Close]

BLAST of Carg10814 vs. TAIR 10
Match: AT2G19730.1 (Ribosomal L28e protein family )

HSP 1 Score: 206.5 bits (524), Expect = 1.5e-53
Identity = 102/143 (71.33%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK N+CFLVK+FGRG++ VQFSKE NNL N++SYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKNNNCFLVKQFGRGNSKVQFSKETNNLTNVHSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
              KD +V+LATTK KKQNKP   +NKS+++KEF  M+KAV NQV DNYYRPDLKKAALA
Sbjct: 61  AADKDQAVVLATTKTKKQNKPKLSVNKSILKKEFPRMSKAVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGAKQRNRQA 143

[Close]

BLAST of Carg10814 vs. TAIR 10
Match: AT2G19730.2 (Ribosomal L28e protein family )

HSP 1 Score: 206.5 bits (524), Expect = 1.5e-53
Identity = 102/143 (71.33%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK N+CFLVK+FGRG++ VQFSKE NNL N++SYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKNNNCFLVKQFGRGNSKVQFSKETNNLTNVHSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
              KD +V+LATTK KKQNKP   +NKS+++KEF  M+KAV NQV DNYYRPDLKKAALA
Sbjct: 61  AADKDQAVVLATTKTKKQNKPKLSVNKSILKKEFPRMSKAVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGAKQRNRQA 143

[Close]

BLAST of Carg10814 vs. TAIR 10
Match: AT2G19730.3 (Ribosomal L28e protein family )

HSP 1 Score: 206.5 bits (524), Expect = 1.5e-53
Identity = 102/143 (71.33%), Postives = 122/143 (85.31%), Query Frame = 0


Query: 1   MANVPGQLVWEIVKKNSCFLVKEFGRGSAGVQFSKEPNNLYNLNSYKHSGLANKKTVTIQ 60
           MA VPGQL+WEIVK N+CFLVK+FGRG++ VQFSKE NNL N++SYKHSGLANKKTVTIQ
Sbjct: 1   MATVPGQLIWEIVKNNNCFLVKQFGRGNSKVQFSKETNNLTNVHSYKHSGLANKKTVTIQ 60

Query: 61  PGGKDLSVLLATTKRKKQNKPASLLNKSVMRKEFSSMAKAVTNQVADNYYRPDLKKAALA 120
              KD +V+LATTK KKQNKP   +NKS+++KEF  M+KAV NQV DNYYRPDLKKAALA
Sbjct: 61  AADKDQAVVLATTKTKKQNKPKLSVNKSILKKEFPRMSKAVANQVVDNYYRPDLKKAALA 120

Query: 121 KLSAVHRSLKIGKSGVKKRNRQA 144
           +LSA+ + L++ KSG K+RNRQA
Sbjct: 121 RLSAISKGLRVAKSGAKQRNRQA 143

The following BLAST results are available for this feature:

BLAST of Carg10814 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946829.1	5.7e-74	100.00	60S ribosomal protein L28-2-like [Cucurbita moschata] >KAG7030478.1 60S ribosoma...	[more]
KAG6599502.1	6.3e-73	98.67	60S ribosomal protein L28-2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022999539.1	2.4e-72	98.00	60S ribosomal protein L28-1-like [Cucurbita maxima] >XP_022999540.1 60S ribosoma...	[more]
XP_023545529.1	2.6e-71	96.67	60S ribosomal protein L28-1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038889445.1	2.5e-66	90.00	60S ribosomal protein L28-1-like [Benincasa hispida]	[more]

BLAST of Carg10814 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M0E2	1.2e-53	72.03	60S ribosomal protein L28-2 OS=Arabidopsis thaliana OX=3702 GN=RPL28C PE=2 SV=1	[more]
O82204	2.2e-52	71.33	60S ribosomal protein L28-1 OS=Arabidopsis thaliana OX=3702 GN=RPL28A PE=1 SV=1	[more]
Q3T0L7	1.3e-12	33.33	60S ribosomal protein L28 OS=Bos taurus OX=9913 GN=RPL28 PE=2 SV=3	[more]
P46779	1.3e-12	33.33	60S ribosomal protein L28 OS=Homo sapiens OX=9606 GN=RPL28 PE=1 SV=3	[more]
P41105	1.3e-12	33.33	60S ribosomal protein L28 OS=Mus musculus OX=10090 GN=Rpl28 PE=1 SV=2	[more]

BLAST of Carg10814 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G510	2.7e-74	100.00	60S ribosomal protein L28-2-like OS=Cucurbita moschata OX=3662 GN=LOC111450772 P...	[more]
A0A6J1KHD5	1.2e-72	98.00	60S ribosomal protein L28-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493869 PE=...	[more]
A0A6J1DMI9	2.7e-66	89.33	60S ribosomal protein L28-1-like OS=Momordica charantia OX=3673 GN=LOC111021927 ...	[more]
A0A6J1FD25	2.0e-64	86.00	60S ribosomal protein L28-2-like OS=Cucurbita moschata OX=3662 GN=LOC111444289 P...	[more]
A0A1S3CAX2	3.4e-64	88.67	60S ribosomal protein L28-1-like OS=Cucumis melo OX=3656 GN=LOC103498417 PE=3 SV...	[more]

BLAST of Carg10814 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29410.1	8.2e-55	72.03	Ribosomal L28e protein family	[more]
AT4G29410.2	8.2e-55	72.03	Ribosomal L28e protein family	[more]
AT2G19730.1	1.5e-53	71.33	Ribosomal L28e protein family	[more]
AT2G19730.2	1.5e-53	71.33	Ribosomal L28e protein family	[more]
AT2G19730.3	1.5e-53	71.33	Ribosomal L28e protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029004	Ribosomal L28e/Mak16	PFAM	PF01778	Ribosomal_L28e	coord: 8..128 e-value: 1.7E-34 score: 119.0
None	No IPR available	GENE3D	3.30.390.110		coord: 6..131 e-value: 1.9E-45 score: 156.3
None	No IPR available	PANTHER	PTHR10544:SF19	60S RIBOSOMAL PROTEIN L28-1-LIKE	coord: 1..150
IPR002672	Ribosomal protein L28e	PANTHER	PTHR10544	60S RIBOSOMAL PROTEIN L28	coord: 1..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10814-RA	Carg10814-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015986	ATP synthesis coupled proton transport
biological_process	GO:0006412	translation
cellular_component	GO:0022625	cytosolic large ribosomal subunit
cellular_component	GO:0000276	mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
cellular_component	GO:0005840	ribosome
molecular_function	GO:0015078	proton transmembrane transporter activity
molecular_function	GO:0003735	structural constituent of ribosome