Carg10780 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10780
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	Carg_Chr05: 12100340 .. 12103826 (-)
RNA-Seq Expression	Carg10780
Synteny	Carg10780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGATGTGTCAGAGAGGTCTGATAGAATGCTAAGGTAAGCGTCCTGGGCATCTGTCTTCTGGGCTTGGCTCAACCTTCTATGTCTGTTGTCTTGTAATAATAATAATGGAGAGGGCCCCCAACGGACCACCTTGATTCCTGCTTTCTCTCTCTCTCACCCCTTTCTCTTCTACTTTTTCAGCATTTAGAACGGCGTACCAAATAGGCTCTTAATCTTAATTTGATTTTGGGAAAGTTTAAAGAGCACACTGTCTAGATCATCAGTTTCGTAGTTCCACTGTGTAACTCTGAAGGTTAAACTTTGAGGGTGAAAATTTGAAGAGAACAATATCCGGTGGACTTGTATCATTATAAAAAGAAACAGGACAAAGGTCCTTTTTGAGAGTGTTGGAAAGCTTCCAAATCAATTAGTATGTAACTGCAGGATATGGAAACTGGGCGCCCTACGCCTGTCTCACTCTCTATGCAGCCCCACACAAACAACTCCTCTTAGACAAATATGTACCTTTTCTTCTCTTCCTTTCTCTCAAGGCAACTGTTTTGATTCATTTTGCTTGTGACCCTTTTCAGTTTACTTTGTAGTTTCATGCTAAAATGGAGCCAAATTGGTAGGTTGTCGTGGGAGTAGAACAAGAAGGGAAATAGAGGATTGTCTCTATTTCTCTTATTTGTGAAAACTTGGAAGAAAAAAAGCTACCTTTAACCGCATTGATTTGATTGCCATTTTTTAGAACCACTATTTGAAGCTCTATATGGTTTAAAGACCTCAATTCTGAAGGTATTTAACAAGAAAAGACTTGACTAAGCAATAGTGTAAAAGCATATGGTCAAAGTGGTGGCCCTTCAGATATTGGAAGAGAGGCTCCACCGTTGTCACCTCCACTGTCCATTAAACAGGGAACAAATGTTTTTATTTATTTGTTTATTGTTCTTTTTGAGATCCAGTAGAATTGTATGGATCCCCGAAGGGCATGGCAAGGGGCAAGGACTCGAGTTTGGGCTGTGGCAGGGGTATTTATTCTTTCAATTGGGCTTTCCGTGAAAGTTGCAAATTGTGCCTAATCTACTCCGAACCGCCTCATCTCCGTTGTTTTGCTGCGGCTTTGTACGACTGGAAGTATGCCGCTAAAATGGTAAAACCCCGGAAACCCCCATCAAAAAAGTGCTCGTTATTTCTTGTGGATTTGTTTCTTGATCTCCTTTCTTTGTTGTTCTTCGATTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGGTGATCGCATTCTTTTTGTAGAGACGATTTTCGGGATTTGATTACCTAAATGAATCTTAAATCTGTCAACAACAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACCCACCCAGGCTTTTCTAGTTCTGATGCATTCTTCATTTCCCCATGTCCAGAAATTCAAATTTGAAATTGATTTTTGTAGCTTTCTCGTCTCTGTTCTTCTGCCCTTTTGTTTATAGTCGTGTGAGATGGATAAATCTTTGGTTACAGACAGTTTGCCTTGTTGGTTTTGTAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGGTAAGGCTCATTTCATCTTTTCATGTCAACTCTGCTCATATGTTATTGCATGTTCTTCTTGTTGGTTTTTTTTTTTTTATGTCTTGTTTTTGTATCTGAGCGAGCAGATTCTTTTCCTATGAGCTGTTGGCCTCTGTGGTTTGTTTCCACGACTTTTCAGAATGGTATGGTATTCTCCACTTTGAGCGTAAGCTCTCATCGCTTTGCCTTTAGTTTCTCTAAAAGGCCTCGTACCAATGGAGATGTATTGCCTTATAAACCAACCACTCCTTAATTAGCTGACGTGGGATTTCTATCCCAATAATCCTCCCCTCGAGCAAAGTACATCATAGAGCCTCCTCTAAGGTCTATAGAGCCCTCAAACAACCTTCCCTTAATTGAGGCTCGACTCCTTCTCTGGAGCCCTCGAACAAATTTCACCATTTGTTTGACACTTGAGTAACTTTTGACTACATCTTTGAGACTCACAACTTCTTTATTCGACATTTGAGGATTCTATTGACAAGGGCATGACTCTGATACCACGTTAGGAACCACAACTCTCTACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTTGTGACTTTGCTTTTGGTTTCTCCAAAATGTCTCGTACCAATGGAAATGTAGTTCCTTATAAACCCATAATCAACCCCTTAATTAGTCGATGTGGGACTCCTCCCCCAACAATCCTCAACATTGTTCTCAACTCAACTCGAGAACAATGAAGTAGAAGGAAAAGGCATCAAATAACTAATCAGTAGGCTCTGTAGTGAAGGATCTTATGTTGAGAAGTAGTAAGATCTGATTAGGATATCTTTGCAAAAGCCTACGTGTAGGCATAGTTATGGAAGTTCGTTATTGTGATCTTCACGCTTTCTGGGTAAATTCCTCCCAAAGAGTTTATTAAGTTTAGAAAGTGTCTTCCAGTTCACCTGGATGCTTGATTATAATATGTAGCCTTTGCTTGGGCTCAATTCCCTAGTGTGGTGCTCATCCTGAAACAAATAAAGCAAAGAAAGATGCCAATTTCTCTCGTCTCAAAAACATTTTGCCATGTTCTTTATTTGAAACTTCTCTGAGATGGCTTGCTTTCATGCTGAAGTTGGAACCTTGACACTTGGTCTCTAGCAAATCTCGATTCAATTCTGTAATTTGTTTCGAAGAACATCATATTGGAAGTACTGTTGAATTTGGAAAGCAGGACTCATATTGAAGTAGAATCTGTATGTCAAATGTGCACGCATGTTATACTTTTTGGCTGCTGTTTGTTGCTCTTCCAATGAAACATTTTGAACACTTGAGACCCTTCACTATTCCTATGTTTAAACCTGTATATTGCAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

mRNA sequence

ATGAATGATGTGTCAGAGAGGTCTGATAGAATGCTAAGTAGAATTGTATGGATCCCCGAAGGGCATGGCAAGGGGCAAGGACTCGAGTTTGGGCTGTGGCAGGGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

Coding sequence (CDS)

ATGAATGATGTGTCAGAGAGGTCTGATAGAATGCTAAGTAGAATTGTATGGATCCCCGAAGGGCATGGCAAGGGGCAAGGACTCGAGTTTGGGCTGTGGCAGGGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

Protein sequence

MNDVSERSDRMLSRIVWIPEGHGKGQGLEFGLWQGVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN

Homology

[Close]

BLAST of Carg10780 vs. NCBI nr
Match: KAG7030510.1 (Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 512.3 bits (1318), Expect = 2.4e-141
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MNDVSERSDRMLSRIVWIPEGHGKGQGLEFGLWQGVLHWQPNAGSTVNSQILNEVSQCVE 60
           MNDVSERSDRMLSRIVWIPEGHGKGQGLEFGLWQGVLHWQPNAGSTVNSQILNEVSQCVE
Sbjct: 1   MNDVSERSDRMLSRIVWIPEGHGKGQGLEFGLWQGVLHWQPNAGSTVNSQILNEVSQCVE 60

Query: 61  SIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEA 120
           SIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEA
Sbjct: 61  SIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEA 120

Query: 121 DFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPIS 180
           DFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPIS
Sbjct: 121 DFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPIS 180

Query: 181 SMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQ 240
           SMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQ
Sbjct: 181 SMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQ 240

Query: 241 LIATVQAVQSARN 254
           LIATVQAVQSARN
Sbjct: 241 LIATVQAVQSARN 253

[Close]

BLAST of Carg10780 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.6 bits (1124), Expect = 7.5e-119
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 426.4 bits (1095), Expect = 1.7e-115
Identity = 211/218 (96.79%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A EFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS++VMEEFFEVWQEAMSKSSLPGRFMHIEPN
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFMHIEPN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 420.6 bits (1080), Expect = 9.4e-114
Identity = 209/218 (95.87%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A EFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEFF+VWQEAMSKSSLPGRFMHIE N
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIESN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 415.6 bits (1067), Expect = 3.0e-112
Identity = 205/218 (94.04%), Postives = 211/218 (96.79%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A EFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFMHIE N
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 6.6e-86
Identity = 156/214 (72.90%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+D      EFPRDF
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKD-QANQLEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+ +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLGDF+LR
Sbjct: 66  LGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F++I+ N
Sbjct: 126 VGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDLN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 250
           F E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 186 FGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of Carg10780 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-76
Identity = 137/212 (64.62%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           +LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+D    A EFPR+F
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQA-EFPREF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           +GIS+PE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLGDF+LR
Sbjct: 66  VGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVP ++E +RG+VME+EYLPISSM  +K++MEEF E+WQEAMSK SLPG+F++ E N
Sbjct: 126 VGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKELN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 248
           F ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 186 FEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of Carg10780 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.9e-09
Identity = 54/196 (27.55%), Postives = 91/196 (46.43%), Query Frame = 0


Query: 52  LNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLII 111
           L E+++ +E + G+K        + Y   + D    +   PR+F  +   E+P K + + 
Sbjct: 13  LPEMTKRIEFLGGIKVSTGTINCSLYLERVPD---GSNLIPREFHLLLYDEKPKKCFTVC 72

Query: 112 RGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIV 171
           R    ++E D  + +I+EK  SYK R        +Y+LGDF +RVG +V      +RGI+
Sbjct: 73  RD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGDFIIRVGPIVFRT--EIRGIL 132

Query: 172 MEIEY----LPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDY--- 231
           +E+EY     P++S     +++ EF          +   G F   EP    +  S Y   
Sbjct: 133 VEVEYTACSTPVTSPFNCTKLLSEFI---------NGNLGPFP--EPIQTSFDFSAYPTL 190

Query: 232 ---YTSQHTAVQYANV 238
              Y+  HT+ QY N+
Sbjct: 193 PKSYSDLHTSYQYVNL 190

[Close]

BLAST of Carg10780 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.6e-119
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.6e-119
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.6e-114
Identity = 209/218 (95.87%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A EFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEFF+VWQEAMSKSSLPGRFMHIE N
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIESN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.5e-112
Identity = 205/218 (94.04%), Postives = 211/218 (96.79%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A EFPRDF
Sbjct: 54  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAEFPRDF 113

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 114 LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 173

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFMHIE N
Sbjct: 174 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESN 233

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 234 FAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of Carg10780 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.5e-112
Identity = 205/218 (94.04%), Postives = 211/218 (96.79%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A EFPRDF
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 66  LGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFMHIE N
Sbjct: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 254
           FAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 186 FAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Carg10780 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 318.5 bits (815), Expect = 4.7e-87
Identity = 156/214 (72.90%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           VLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+D      EFPRDF
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKD-QANQLEFPRDF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           LGIS+ +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLGDF+LR
Sbjct: 66  LGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F++I+ N
Sbjct: 126 VGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDLN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 250
           F E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 186 FGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of Carg10780 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 287.3 bits (734), Expect = 1.2e-77
Identity = 137/212 (64.62%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95
           +LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+D    A EFPR+F
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQA-EFPREF 65

Query: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155
           +GIS+PE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLGDF+LR
Sbjct: 66  VGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRLR 125

Query: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215
           VGKVVP ++E +RG+VME+EYLPISSM  +K++MEEF E+WQEAMSK SLPG+F++ E N
Sbjct: 126 VGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKELN 185

Query: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 248
           F ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 186 FEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of Carg10780 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 82.0 bits (201), Expect = 7.4e-16
Identity = 42/68 (61.76%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 182 MEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQ-YANVMAQ 241
           ME +++VMEEF E+W EA++K SLPG+F++ + NF E+GL D YT QHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 242 LIATVQAV 249
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of Carg10780 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030510.1	2.4e-141	100.00	Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argy...	[more]
XP_022946745.1	7.5e-119	100.00	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]
XP_038889134.1	1.7e-115	96.79	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_004141069.1	9.4e-114	95.87	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_008459711.1	3.0e-112	94.04	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]

BLAST of Carg10780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	6.6e-86	72.90	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	1.6e-76	64.62	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	2.9e-09	27.55	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of Carg10780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEU1	3.6e-119	100.00	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	3.6e-119	100.00	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]
A0A0A0LHQ1	4.6e-114	95.87	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A5D3CH02	1.5e-112	94.04	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	1.5e-112	94.04	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Carg10780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	4.7e-87	72.90	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	1.2e-77	64.62	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	7.4e-16	61.76	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 34..238 e-value: 5.3E-41 score: 140.4
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 36..247
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 36..247

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10780-RA	Carg10780-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003713	transcription coactivator activity
molecular_function	GO:0003712	transcription coregulator activity