Carg10438 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10438
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectate lyase
LocationCarg_Chr03: 8811951 .. 8814594 (-)
RNA-Seq ExpressionCarg10438
SyntenyCarg10438
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTGAGTGGGGTGGGAGACTGAGAGAGAGAGAGAGAGAGTTTACCATTTTTGCGCCAAAATGTTGACCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCTGCTGGCCGCTAGGTGCCGCCGCCGCCGCTCTCAACCTCACTCTACCCGGCCAGCATCCGAGCCCTGAACTGGTTGCTCAAGAAGTTCACAGGTCCATCAATTTTCCATTCTTTCCACAAATGTTGTTTTAGAATCACTATGTTCTTGGCTTGGCTCTCACTCTAACTGTCTGCATTTTGGAACTTTTAGCTACATTGTTCATTTTCTCTACTTTAATGGCCAGTTTCAAGATTTTTATGCTTCTTTTTTGTAGGATTTCATGTATTAGAAGTACAAAATCAAGTTTAAAGCCCGGCCTTTTTGTTTCTATGTTCTTGGTTGCTCTGTTTTTCATGGTCTTGTTTATTTCAGCTCTGTGTATCTACAGTGTCTTCAGTTTTGAGTTTTCTTGACCAAATTATTAGGCATTAACACATAAAACTTATGCTTAAATCTTACTCTTCCAATCTTGTTCTGCTCAGTAACTATTATCTTTCTGACCCATTGTATAAACTTTTAGGTGCATTGTTGGATTTCTTCACAAATTGAATCATTTTTTAAATCTTAACCTACTTTTACAGATTTAGTAATTATAAGAAAAAGAAAATGCCATTCAAATCTACAGTTTTCGATTTCATTTCATATCTTAATCTTCATGCACTGTTCTTTGTTAGATTTCGGCATTTTTAAAAGAAGAATGCTGCATAATCCTTCTCCTTCGCTACAGTTATTGGCTACTCTGTTTTTGTTTCTGTTTTTGGTGAAAAGTATTGAAAATCCCCAAGTTTTTCTTTGTCTATCCTTCTTTCAGATCATTTGTCGAATTTCAAGGTATATCTTCGCAGATTCAACCATTTATGGAGCTGACATTTAGATTATACTGTCTAGGTTTAATGCTAAGTTCCTTGAAAACCCTAAATTATGGAAGGAAATTGTTACCTGTTGCAGGAAAGTGAATGCTTCTGTAACGAGAAGGCAATTGCTCCAAAGCTTAGAGAAAGACGAATCTTCTTCTTGTTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAACTGGCCAAACAACCGCCAGCGTCTCGCCGATTGCGCCATAGGCTTCGGCCAGTACGCACTCGGCGGCAAGAACGGCGAGTTCTACATCGTCACAGACGATTCCGACGACGATGCAGTCAATCCAAAGCCCGGAACGTTACGATACGCCGTGATACAGCCTCAACCTCTCTGGATCGTCTTCCCGGCCAACATGCTCATTAAACTCAAACAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACATTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGTTATCCATCAGGTAACGAAATCGAAAAACCTAATTTACCTTCCTGTACACCAAATGTTCGACGAAATGCATCGATCTGAAGCTTGGCTCTGCCTCTGGCTCTGCAGGTAACACCATGGTCCGATCGAGTCCGACGCATTACGGATACCGCACGAAATCGGACGGCGACGGGATATCCATCTTCGGATCGAAGGACATATGGATTGACCATTGCTCACTCTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACAATTTCAAACAATTATTTCTCACATCACGATGAGGTTATGCTTTTGGGTCACAGCGATAGCTACTTGCCGGATTCCGGAATGCAGGTAAGTTCCCCATCCTCTACTCCTTGTTTCTCTCCGTAAATTACTTAAATTAGTTTAATTAAAAGTTAATCCTCATTTTTTACTAATTTATGTCGTATTTCAAATTTAGTTAATCTATTAAATTTTAATCTTTTTTGAAATTACAATATTACCCTTATTCTTTTATAAACATTTTTTAATAATAAAATAAGGTTTTTGCTTTTCATAAAGGTAACAATAGCGTTCAACCACTTCGGCGAGAAGCTAGTTCAAAGGATGCCGCGATGCCGGCGAGGGTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGGAGCCCCACCATCAACAGCCAAGGCAACCGCTACACCGCCCCATACGACCGCAACGCCAAGGAGGTGACCAAGCGGGTTGAAACATCGGAGTCCGAGTGGCGGGGTTGGAACTGGCGCTCCGACGGCGACATCCTCGTTAACGGCGCCTTCTTCGTTACCTCTGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAATCAGTCGCCCTGATCGACCAGCTCACTTGGCACGCTGGCCCGCTTGGCGTCGGCGGCAGGGATAACAATTTGGGGATGTGGACCACTGGCCCCAATGGAGGTGGAGGCTTTGTCATTGGGTCTGGTCCGGATTACACTGACGACATGTCCGAAAGCTTGTCTCGGATTGGGTCTTCTTATTTTGTCATGGTATTGTCTGTTTTGTTGTGTTCTTTTTGCGCATTGGCACTCGCATTTGTGCAATGA

mRNA sequence

GAGTGAGTGGGGTGGGAGACTGAGAGAGAGAGAGAGAGAGTTTACCATTTTTGCGCCAAAATGTTGACCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCTGCTGGCCGCTAGGTGCCGCCGCCGCCGCTCTCAACCTCACTCTACCCGGCCAGCATCCGAGCCCTGAACTGGTTGCTCAAGAAGTTCACAGGTTTAATGCTAAGTTCCTTGAAAACCCTAAATTATGGAAGGAAATTGTTACCTGTTGCAGGAAAGTGAATGCTTCTGTAACGAGAAGGCAATTGCTCCAAAGCTTAGAGAAAGACGAATCTTCTTCTTGTTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAACTGGCCAAACAACCGCCAGCGTCTCGCCGATTGCGCCATAGGCTTCGGCCAGTACGCACTCGGCGGCAAGAACGGCGAGTTCTACATCGTCACAGACGATTCCGACGACGATGCAGTCAATCCAAAGCCCGGAACGTTACGATACGCCGTGATACAGCCTCAACCTCTCTGGATCGTCTTCCCGGCCAACATGCTCATTAAACTCAAACAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACATTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGTTATCCATCAGGTAACACCATGGTCCGATCGAGTCCGACGCATTACGGATACCGCACGAAATCGGACGGCGACGGGATATCCATCTTCGGATCGAAGGACATATGGATTGACCATTGCTCACTCTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACAATTTCAAACAATTATTTCTCACATCACGATGAGGTTATGCTTTTGGGTCACAGCGATAGCTACTTGCCGGATTCCGGAATGCAGGTAACAATAGCGTTCAACCACTTCGGCGAGAAGCTAGTTCAAAGGATGCCGCGATGCCGGCGAGGGTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGGAGCCCCACCATCAACAGCCAAGGCAACCGCTACACCGCCCCATACGACCGCAACGCCAAGGAGGTGACCAAGCGGGTTGAAACATCGGAGTCCGAGTGGCGGGGTTGGAACTGGCGCTCCGACGGCGACATCCTCGTTAACGGCGCCTTCTTCGTTACCTCTGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAATCAGTCGCCCTGATCGACCAGCTCACTTGGCACGCTGGCCCGCTTGGCGTCGGCGGCAGGGATAACAATTTGGGGATGTGGACCACTGGCCCCAATGGAGGTGGAGGCTTTGTCATTGGGTCTGGTCCGGATTACACTGACGACATGTCCGAAAGCTTGTCTCGGATTGGGTCTTCTTATTTTGTCATGGTATTGTCTGTTTTGTTGTGTTCTTTTTGCGCATTGGCACTCGCATTTGTGCAATGA

Coding sequence (CDS)

ATGTTGACCACAACCTGCATTGTGCTGCTAGCTTTGCTGCTACCCTCCTGCTGGCCGCTAGGTGCCGCCGCCGCCGCTCTCAACCTCACTCTACCCGGCCAGCATCCGAGCCCTGAACTGGTTGCTCAAGAAGTTCACAGGTTTAATGCTAAGTTCCTTGAAAACCCTAAATTATGGAAGGAAATTGTTACCTGTTGCAGGAAAGTGAATGCTTCTGTAACGAGAAGGCAATTGCTCCAAAGCTTAGAGAAAGACGAATCTTCTTCTTGTTACACCGGGAACCCCATCGACGACTGCTGGAAATGCGATCCAAACTGGCCAAACAACCGCCAGCGTCTCGCCGATTGCGCCATAGGCTTCGGCCAGTACGCACTCGGCGGCAAGAACGGCGAGTTCTACATCGTCACAGACGATTCCGACGACGATGCAGTCAATCCAAAGCCCGGAACGTTACGATACGCCGTGATACAGCCTCAACCTCTCTGGATCGTCTTCCCGGCCAACATGCTCATTAAACTCAAACAGGAACTCATCTTCAACAGCTACAAAACCCTAGACGGCCGCGGCGCCAATGTCCACATCGTCGGCGGTGGCTGCATTACATTGCAGTACATAAGCAACGTCATCATCCACAACATCCACATCCACCATTGTTATCCATCAGGTAACACCATGGTCCGATCGAGTCCGACGCATTACGGATACCGCACGAAATCGGACGGCGACGGGATATCCATCTTCGGATCGAAGGACATATGGATTGACCATTGCTCACTCTCGCATTGCAAAGATGGGCTGATTGATGCGGTGATGGGCTCCACCGGAATCACAATTTCAAACAATTATTTCTCACATCACGATGAGGTTATGCTTTTGGGTCACAGCGATAGCTACTTGCCGGATTCCGGAATGCAGGTAACAATAGCGTTCAACCACTTCGGCGAGAAGCTAGTTCAAAGGATGCCGCGATGCCGGCGAGGGTACATTCACGTCGTCAACAACGACTTCACCCAATGGGAAATGTACGCAATCGGCGGCAGTGGGAGCCCCACCATCAACAGCCAAGGCAACCGCTACACCGCCCCATACGACCGCAACGCCAAGGAGGTGACCAAGCGGGTTGAAACATCGGAGTCCGAGTGGCGGGGTTGGAACTGGCGCTCCGACGGCGACATCCTCGTTAACGGCGCCTTCTTCGTTACCTCTGGCCAAGGCCTCGAAGTCAAGTACGAGAAGGCCTACAGCGTCGAGCCCAAATCAGTCGCCCTGATCGACCAGCTCACTTGGCACGCTGGCCCGCTTGGCGTCGGCGGCAGGGATAACAATTTGGGGATGTGGACCACTGGCCCCAATGGAGGTGGAGGCTTTGTCATTGGGTCTGGTCCGGATTACACTGACGACATGTCCGAAAGCTTGTCTCGGATTGGGTCTTCTTATTTTGTCATGGTATTGTCTGTTTTGTTGTGTTCTTTTTGCGCATTGGCACTCGCATTTGTGCAATGA

Protein sequence

MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWKEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYFVMVLSVLLCSFCALALAFVQ
Homology
BLAST of Carg10438 vs. NCBI nr
Match: KAG7034543.1 (putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.7e-300
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
           EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF
Sbjct: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN
Sbjct: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
           VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF
Sbjct: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480

Query: 481 VMVLSVLLCSFCALALAFVQ 501
           VMVLSVLLCSFCALALAFVQ
Sbjct: 481 VMVLSVLLCSFCALALAFVQ 500

BLAST of Carg10438 vs. NCBI nr
Match: KAG6604393.1 (putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 984.9 bits (2545), Expect = 2.5e-283
Identity = 480/500 (96.00%), Postives = 480/500 (96.00%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH              
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH-------------- 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF
Sbjct: 61  ------RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN
Sbjct: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
           VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF
Sbjct: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480

Query: 481 VMVLSVLLCSFCALALAFVQ 501
           VMVLSVLLCSFCALALAFVQ
Sbjct: 481 VMVLSVLLCSFCALALAFVQ 480

BLAST of Carg10438 vs. NCBI nr
Match: XP_022925720.1 (probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate lyase 12 [Cucurbita moschata])

HSP 1 Score: 983.0 bits (2540), Expect = 9.4e-283
Identity = 479/500 (95.80%), Postives = 479/500 (95.80%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH              
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH-------------- 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF
Sbjct: 61  ------RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN
Sbjct: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
           VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGS YF
Sbjct: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSPYF 480

Query: 481 VMVLSVLLCSFCALALAFVQ 501
           VMVLSVLLCSFCALALAFVQ
Sbjct: 481 VMVLSVLLCSFCALALAFVQ 480

BLAST of Carg10438 vs. NCBI nr
Match: XP_022978930.1 (probable pectate lyase 12 [Cucurbita maxima])

HSP 1 Score: 974.5 bits (2518), Expect = 3.3e-280
Identity = 475/501 (94.81%), Postives = 478/501 (95.41%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGA-AAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLW 60
           MLTTTCIVLLALLLPSCWPLGA AAAALNLTLPGQHPSPELVAQEVH             
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGASAAAALNLTLPGQHPSPELVAQEVH------------- 60

Query: 61  KEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIG 120
                  RKVNASVTRRQLLQSLEKDES SCYTGNPIDDCWKCDPNWPNNRQRLADCAIG
Sbjct: 61  -------RKVNASVTRRQLLQSLEKDESPSCYTGNPIDDCWKCDPNWPNNRQRLADCAIG 120

Query: 121 FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF 180
           FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF
Sbjct: 121 FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF 180

Query: 181 NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS 240
           NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS
Sbjct: 181 NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS 240

Query: 241 DGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL 300
           DGDGISIFGSKD+WIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL
Sbjct: 241 DGDGISIFGSKDVWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL 300

Query: 301 PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY 360
           PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY
Sbjct: 301 PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY 360

Query: 361 TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK 420
           TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK
Sbjct: 361 TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK 420

Query: 421 SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSY 480
           SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGF+IGSGPDYTDDMSESLSRIGS Y
Sbjct: 421 SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFIIGSGPDYTDDMSESLSRIGSPY 480

Query: 481 FVMVLSVLLCSFCALALAFVQ 501
           FVMV+SVLLCSFCALALAFVQ
Sbjct: 481 FVMVVSVLLCSFCALALAFVQ 481

BLAST of Carg10438 vs. NCBI nr
Match: XP_023544235.1 (probable pectate lyase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 478/502 (95.22%), Postives = 478/502 (95.22%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLG--AAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKL 60
           MLTTTCIVLLALLLPSC PLG  AAAAALNLTLPGQHPSPELVAQEVH            
Sbjct: 1   MLTTTCIVLLALLLPSCSPLGASAAAAALNLTLPGQHPSPELVAQEVH------------ 60

Query: 61  WKEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAI 120
                   RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAI
Sbjct: 61  --------RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAI 120

Query: 121 GFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELI 180
           GFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELI
Sbjct: 121 GFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELI 180

Query: 181 FNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTK 240
           FNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTK
Sbjct: 181 FNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTK 240

Query: 241 SDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSY 300
           SDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSY
Sbjct: 241 SDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSY 300

Query: 301 LPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNR 360
           LPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNR
Sbjct: 301 LPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNR 360

Query: 361 YTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEP 420
           YTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEP
Sbjct: 361 YTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEP 420

Query: 421 KSVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSS 480
           KSVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGS 
Sbjct: 421 KSVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSP 480

Query: 481 YFVMVLSVLLCSFCALALAFVQ 501
           YFVMVLSVLLCSFCALALAFVQ
Sbjct: 481 YFVMVLSVLLCSFCALALAFVQ 482

BLAST of Carg10438 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 718.8 bits (1854), Expect = 4.3e-206
Identity = 361/492 (73.37%), Postives = 399/492 (81.10%), Query Frame = 0

Query: 1   MLTTTCIVL---LALLLPSCWPLGAAAAALNLT-LPGQHPSPELVAQEVHRFNAKFLENP 60
           ML  +CIVL   L LL+P         + LN T L   HP PELVA +V           
Sbjct: 2   MLQRSCIVLFFSLFLLVPQ-----MVFSMLNRTLLLIPHPDPELVAYQVQ---------- 61

Query: 61  KLWKEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADC 120
             W        KVNAS+TRRQ L + ++  S+ C TGNPIDDCWKCDPNWPNNRQ LADC
Sbjct: 62  --W--------KVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADC 121

Query: 121 AIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQE 180
            IGFGQYALGGK G+FY VTD SDDDAVNPKPGTLRY VIQ +PLWIVFP+NM+IKLKQE
Sbjct: 122 GIGFGQYALGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQE 181

Query: 181 LIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYR 240
           LIFNSYKTLDGRGANVHIVGGGCITLQY+SN+IIHNIHIHHCY SGNT VRSSPTHYG+R
Sbjct: 182 LIFNSYKTLDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFR 241

Query: 241 TKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD 300
           TKSDGDGISIFGSKDIWIDHCSLS CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD
Sbjct: 242 TKSDGDGISIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSD 301

Query: 301 SYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQG 360
            Y PDSGMQVTIAFNHFGEKL+QRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQG
Sbjct: 302 HYEPDSGMQVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQG 361

Query: 361 NRYTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSV 420
           NRYTAP +  AKEVTKRVET + +W+GWNWRS+GDILVNGAFFV SG+G E++YEKAYSV
Sbjct: 362 NRYTAPTNPFAKEVTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSV 421

Query: 421 EPKSVALIDQLTWHAGPLGVGGRDNNLGMW-TTGPNGGGGFVIGSGPDYTDDMS--ESLS 480
           EPKS + I Q+T+H+G LGVGGR+NNLGMW TTG  G  G  + S  DYTD+MS   S +
Sbjct: 422 EPKSASFITQITFHSGVLGVGGRNNNLGMWTTTGSEGTSG--LDSYNDYTDEMSGAGSTN 466

Query: 481 RIGSSYFVMVLS 486
           R+  S  V +LS
Sbjct: 482 RLSFSVLVFLLS 466

BLAST of Carg10438 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 5.7e-158
Identity = 291/490 (59.39%), Postives = 343/490 (70.00%), Query Frame = 0

Query: 13  LLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWKEIVTCCRKVNAS 72
           LL   + L +A   LNLTLP QHPSP+ VA  V                     R VN S
Sbjct: 12  LLCLFFTLLSATKPLNLTLPHQHPSPDSVALHV--------------------IRSVNES 71

Query: 73  VTRRQL------LQSLEKDESSSCYTGNPIDDCWKC-DPNWPNNRQRLADCAIGFGQYAL 132
           + RRQL        S     SSSC TGNPIDDCW+C D +W  NRQRLADC+IGFG   L
Sbjct: 72  LARRQLSSPSSSSSSSSSSSSSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTL 131

Query: 133 GGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTL 192
           GGKNG+ Y+VTD SD++  NP PGTLRY VIQ +PLWIVF +NMLI+LKQELI NSYKTL
Sbjct: 132 GGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTL 191

Query: 193 DGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGIS 252
           DGRG+ VHI G GC+TLQY+ ++IIHN+HI+ C PS           +  R +SDGDGIS
Sbjct: 192 DGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCKPSAG---------FEKRGRSDGDGIS 251

Query: 253 IFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQ 312
           IFGS+ IW+DHCS+SHC DGLIDAVMGST ITISNNYF+HHDEVMLLGH D+Y PD+GMQ
Sbjct: 252 IFGSQKIWVDHCSMSHCTDGLIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAPDTGMQ 311

Query: 313 VTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDR 372
           VTIAFNHFG+ LVQRMPRCRRGYIHVVNNDFT+W+MYAIGGSG+PTINSQGNRY+AP D 
Sbjct: 312 VTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDP 371

Query: 373 NAKEVTKRVET-SESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALI 432
           +AKEVTKRV++  + EW  WNWR++GD++ NGAFFV SG+G+   Y KA SV+PK+ +L+
Sbjct: 372 SAKEVTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVDPKAASLV 431

Query: 433 DQLTWHAGPLGVGGRDN-----NLGMWTTGPNGGGGFVIGSGPDYTDDM------SESLS 484
           DQLT +AG  G G RD+     N      G  GGGG   GS     D M      S S S
Sbjct: 432 DQLTRNAGVFG-GPRDDQGQSGNSYSPYGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSS 471

BLAST of Carg10438 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 4.7e-152
Identity = 243/347 (70.03%), Postives = 286/347 (82.42%), Query Frame = 0

Query: 89  SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKP 148
           SC TGNPIDDCW+CD NW  NR+RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKP
Sbjct: 117 SCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKP 176

Query: 149 GTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNV 208
           GTLR+AVIQ +PLWIVF  +M+I+LKQELI NS+KT+D RG+NVHI  G CIT+Q+I+NV
Sbjct: 177 GTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNV 236

Query: 209 IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLID 268
           IIH +HIH C P+GN MVRSSP+H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+D
Sbjct: 237 IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVD 296

Query: 269 AVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGY 328
           AVMGST IT+SNN+F+HH+EVMLLGHSDSY  D  MQVTIA+NHFGE LVQRMPRCR GY
Sbjct: 297 AVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGY 356

Query: 329 IHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRS 388
            HVVNND+T WEMYAIGGS  PTINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS
Sbjct: 357 FHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRS 416

Query: 389 DGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLG 436
           +GD+L+NGAFF  SG G    Y +A S+  K  +++D +T  AG LG
Sbjct: 417 EGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALG 463

BLAST of Carg10438 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 529.3 bits (1362), Expect = 4.9e-149
Identity = 235/346 (67.92%), Postives = 284/346 (82.08%), Query Frame = 0

Query: 89  SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKP 148
           +C TGNPIDDCW+CDPNW  NR+RLADC IGFG+ A+GG++G FY+VTD  DD+ VNP+P
Sbjct: 76  TCGTGNPIDDCWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRP 135

Query: 149 GTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNV 208
           GTLR+AVIQ +PLWIVF  +M+I+LKQELI NS+KT+DGRGANVHI  GGCIT+Q+++NV
Sbjct: 136 GTLRHAVIQDRPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNV 195

Query: 209 IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLID 268
           I+H +HIH C P+GN MVRSS TH+G+RT +DGD ISIFGS  +WIDH SLSHC DGL+D
Sbjct: 196 IVHGLHIHDCKPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVD 255

Query: 269 AVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGY 328
           AVMGST ITISNN+ +HH+EVMLLGHSDSY+ D  MQVTIA+NHFG  L+QRMPRCR GY
Sbjct: 256 AVMGSTAITISNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGY 315

Query: 329 IHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRS 388
            HVVNND+T WEMYAIGGS +PTINSQGNRY AP +  AKEVTKRV+T  S W+GWNWRS
Sbjct: 316 FHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRS 375

Query: 389 DGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPL 435
           +GD+L NGA+F +SG      Y +A S+  KS +L+  +T  AG L
Sbjct: 376 EGDLLQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSDAGAL 421

BLAST of Carg10438 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 522.7 bits (1345), Expect = 4.6e-147
Identity = 244/406 (60.10%), Postives = 298/406 (73.40%), Query Frame = 0

Query: 29  LTLPGQHPSPELVAQEVHRFNAKFLENPKLWKEIVTCCRKVNASVTRRQLLQSLEKDESS 88
           L L    P PE V +EVH                    + +NASV  R+ L  L      
Sbjct: 21  LFLSSPVPDPESVVEEVH--------------------KSINASVAGRRKLGYL------ 80

Query: 89  SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKP 148
           SC TGNPIDDCW+CDP+W  +RQRLADCAIGFG+ A+GG++G  Y+VTD  +D+ V+PKP
Sbjct: 81  SCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKP 140

Query: 149 GTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNV 208
           GTLR+AV+Q +PLWI+F  +M I+LK+ELI NS+KT+DGRGA+VHI GG CIT+QY++N+
Sbjct: 141 GTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNI 200

Query: 209 IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLID 268
           IIH IHIH C   GN MVRSSP H+G+RT SDGDG+SIFG   +W+DHCS S+C+DGLID
Sbjct: 201 IIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLID 260

Query: 269 AVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGY 328
           A+MGST IT+SNN+ +HHD+VMLLGHSD+Y  D  MQVTIAFNHFGE LVQRMPRCR GY
Sbjct: 261 AIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGY 320

Query: 329 IHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRS 388
            HVVNND+T WEMYAIGGS +PTINSQGNR+ AP  R +KEVTK  +  ESEW+ WNWRS
Sbjct: 321 FHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRS 380

Query: 389 DGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPL 435
            GD+L+NGAFF  SG      Y KA S+  K  +L+  LT  +G L
Sbjct: 381 SGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGAL 400

BLAST of Carg10438 vs. ExPASy TrEMBL
Match: A0A6J1EJ04 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.6e-283
Identity = 479/500 (95.80%), Postives = 479/500 (95.80%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH              
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVH-------------- 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF
Sbjct: 61  ------RKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN
Sbjct: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
           VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGS YF
Sbjct: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSPYF 480

Query: 481 VMVLSVLLCSFCALALAFVQ 501
           VMVLSVLLCSFCALALAFVQ
Sbjct: 481 VMVLSVLLCSFCALALAFVQ 480

BLAST of Carg10438 vs. ExPASy TrEMBL
Match: A0A6J1IRN3 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.6e-280
Identity = 475/501 (94.81%), Postives = 478/501 (95.41%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGA-AAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLW 60
           MLTTTCIVLLALLLPSCWPLGA AAAALNLTLPGQHPSPELVAQEVH             
Sbjct: 1   MLTTTCIVLLALLLPSCWPLGASAAAALNLTLPGQHPSPELVAQEVH------------- 60

Query: 61  KEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIG 120
                  RKVNASVTRRQLLQSLEKDES SCYTGNPIDDCWKCDPNWPNNRQRLADCAIG
Sbjct: 61  -------RKVNASVTRRQLLQSLEKDESPSCYTGNPIDDCWKCDPNWPNNRQRLADCAIG 120

Query: 121 FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF 180
           FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF
Sbjct: 121 FGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIF 180

Query: 181 NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS 240
           NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS
Sbjct: 181 NSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKS 240

Query: 241 DGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL 300
           DGDGISIFGSKD+WIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL
Sbjct: 241 DGDGISIFGSKDVWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYL 300

Query: 301 PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY 360
           PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY
Sbjct: 301 PDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRY 360

Query: 361 TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK 420
           TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK
Sbjct: 361 TAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPK 420

Query: 421 SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSY 480
           SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGF+IGSGPDYTDDMSESLSRIGS Y
Sbjct: 421 SVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFIIGSGPDYTDDMSESLSRIGSPY 480

Query: 481 FVMVLSVLLCSFCALALAFVQ 501
           FVMV+SVLLCSFCALALAFVQ
Sbjct: 481 FVMVVSVLLCSFCALALAFVQ 481

BLAST of Carg10438 vs. ExPASy TrEMBL
Match: A0A6J1I6R7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111471237 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 435/499 (87.17%), Postives = 447/499 (89.58%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           M+   CIVLLALLL S   L     ALNLTLPGQHPSPELVAQEVH              
Sbjct: 1   MVAAICIVLLALLLHS---LSPPCTALNLTLPGQHPSPELVAQEVH-------------- 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVN S+TRRQL+Q+ E DESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGF
Sbjct: 61  ------RKVNDSMTRRQLIQNSENDESSSCYTGNPIDDCWKCDSNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYALGGKNGEFY+VTDDSDDD VNP+PGTLRYAVIQPQPLWIVFPANMLIKL QELIFN
Sbjct: 121 GQYALGGKNGEFYVVTDDSDDDPVNPEPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISN+IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNIIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSY P
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYSP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVET+ESEWRGWNWRSDGDILVNGAFF+TSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFITSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
            ALIDQLTWHAGPLGVG RDNNLGMWTTG NGGGG+V+GSGPDYTDDMSESL RI   YF
Sbjct: 421 AALIDQLTWHAGPLGVGSRDNNLGMWTTGSNGGGGYVLGSGPDYTDDMSESLWRIRFRYF 471

Query: 481 VMVLSVLLCSFCALALAFV 500
           VMV      SFCALALAFV
Sbjct: 481 VMVF-----SFCALALAFV 471

BLAST of Carg10438 vs. ExPASy TrEMBL
Match: A0A0A0KIG0 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.4e-254
Identity = 445/506 (87.94%), Postives = 453/506 (89.53%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAA---AALNLTLPGQHPSPELVAQEVHRFNAKFLENPK 60
           ML TTCIVLLALLLPS  PL AAA   AALNLTLPGQHPSPELVAQEVH           
Sbjct: 1   MLATTCIVLLALLLPSRLPLRAAATTSAALNLTLPGQHPSPELVAQEVH----------- 60

Query: 61  LWKEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCA 120
                    RKVNAS+TRRQLLQ  EKDESSSCYTGNPIDDCWKCD NWPNNRQRLADCA
Sbjct: 61  ---------RKVNASLTRRQLLQISEKDESSSCYTGNPIDDCWKCDRNWPNNRQRLADCA 120

Query: 121 IGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQEL 180
           IGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL QEL
Sbjct: 121 IGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLSQEL 180

Query: 181 IFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRT 240
           IFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHC+PSGNTMVRSSPTHYGYRT
Sbjct: 181 IFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCHPSGNTMVRSSPTHYGYRT 240

Query: 241 KSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDS 300
           KSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD+
Sbjct: 241 KSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDN 300

Query: 301 YLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGN 360
           Y PDSGMQVTIAFNHFGEKLVQRMPRCR GYIHVVNNDFTQWEMYAIGGSG+PTINSQGN
Sbjct: 301 YWPDSGMQVTIAFNHFGEKLVQRMPRCRLGYIHVVNNDFTQWEMYAIGGSGNPTINSQGN 360

Query: 361 RYTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVE 420
           RYTAPYDRNAKEVTKRVETSESEWRGWNWRS+GDILVNGAFFVTSGQGLEVKYEKAYSVE
Sbjct: 361 RYTAPYDRNAKEVTKRVETSESEWRGWNWRSEGDILVNGAFFVTSGQGLEVKYEKAYSVE 420

Query: 421 PKSVALIDQLTWHAGPLGVGGRDNNLGMWTTGPNG-GGGFVIGSGPDYTDDMSESLSRIG 480
           PKS ALIDQLTWHAGPLGVGGRDNNLGMWTTGPNG GGGF +GSG DYTDDMSES     
Sbjct: 421 PKSAALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGNGGGFGLGSGMDYTDDMSESC---- 479

Query: 481 SSYFVMVLSVLLCSFC---ALALAFV 500
              FV V SVLL SFC    + +AFV
Sbjct: 481 ---FVRVFSVLLWSFCWALVVVVAFV 479

BLAST of Carg10438 vs. ExPASy TrEMBL
Match: A0A6J1HI82 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463214 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.8e-254
Identity = 435/499 (87.17%), Postives = 446/499 (89.38%), Query Frame = 0

Query: 1   MLTTTCIVLLALLLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWK 60
           M+   C VLLALLL S   L     ALNL+LPGQHPSPELVAQEVH              
Sbjct: 1   MVAAICTVLLALLLHS---LSPPCTALNLSLPGQHPSPELVAQEVH-------------- 60

Query: 61  EIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADCAIGF 120
                 RKVN S+TRR+L+Q+ E DESSSCYTGNPIDDCWKCD NWPNNRQRLADCAIGF
Sbjct: 61  ------RKVNDSITRRRLIQNSENDESSSCYTGNPIDDCWKCDSNWPNNRQRLADCAIGF 120

Query: 121 GQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFN 180
           GQYA GGKNGEFY+VTDDSDDD VNP+PGTLRYAVIQPQPLWIVFPANMLIKL QELIFN
Sbjct: 121 GQYASGGKNGEFYVVTDDSDDDPVNPEPGTLRYAVIQPQPLWIVFPANMLIKLSQELIFN 180

Query: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240
           SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD
Sbjct: 181 SYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSD 240

Query: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLP 300
           GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSY P
Sbjct: 241 GDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYSP 300

Query: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYT 360
           DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQGNRYT
Sbjct: 301 DSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYT 360

Query: 361 APYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420
           APYDRNAKEVTKRVET+ESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS
Sbjct: 361 APYDRNAKEVTKRVETAESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKS 420

Query: 421 VALIDQLTWHAGPLGVGGRDNNLGMWTTGPNGGGGFVIGSGPDYTDDMSESLSRIGSSYF 480
            ALIDQLTWHAGPLGVG RDNNLGMWTTG NGGGGFV+GSGPDYTDDMSESLSRI   YF
Sbjct: 421 AALIDQLTWHAGPLGVGSRDNNLGMWTTGSNGGGGFVLGSGPDYTDDMSESLSRIRFRYF 471

Query: 481 VMVLSVLLCSFCALALAFV 500
           VMV      SFCALALAFV
Sbjct: 481 VMVF-----SFCALALAFV 471

BLAST of Carg10438 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 718.8 bits (1854), Expect = 3.1e-207
Identity = 361/492 (73.37%), Postives = 399/492 (81.10%), Query Frame = 0

Query: 1   MLTTTCIVL---LALLLPSCWPLGAAAAALNLT-LPGQHPSPELVAQEVHRFNAKFLENP 60
           ML  +CIVL   L LL+P         + LN T L   HP PELVA +V           
Sbjct: 2   MLQRSCIVLFFSLFLLVPQ-----MVFSMLNRTLLLIPHPDPELVAYQVQ---------- 61

Query: 61  KLWKEIVTCCRKVNASVTRRQLLQSLEKDESSSCYTGNPIDDCWKCDPNWPNNRQRLADC 120
             W        KVNAS+TRRQ L + ++  S+ C TGNPIDDCWKCDPNWPNNRQ LADC
Sbjct: 62  --W--------KVNASITRRQALDTTDQAGSTPCITGNPIDDCWKCDPNWPNNRQGLADC 121

Query: 121 AIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQE 180
            IGFGQYALGGK G+FY VTD SDDDAVNPKPGTLRY VIQ +PLWIVFP+NM+IKLKQE
Sbjct: 122 GIGFGQYALGGKGGQFYFVTDSSDDDAVNPKPGTLRYGVIQEEPLWIVFPSNMMIKLKQE 181

Query: 181 LIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYR 240
           LIFNSYKTLDGRGANVHIVGGGCITLQY+SN+IIHNIHIHHCY SGNT VRSSPTHYG+R
Sbjct: 182 LIFNSYKTLDGRGANVHIVGGGCITLQYVSNIIIHNIHIHHCYQSGNTNVRSSPTHYGFR 241

Query: 241 TKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSD 300
           TKSDGDGISIFGSKDIWIDHCSLS CKDGLIDAVMGSTGITISNN+FSHH+EVMLLGHSD
Sbjct: 242 TKSDGDGISIFGSKDIWIDHCSLSRCKDGLIDAVMGSTGITISNNFFSHHNEVMLLGHSD 301

Query: 301 SYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQG 360
            Y PDSGMQVTIAFNHFGEKL+QRMPRCRRGYIHVVNNDFTQWEMYAIGGSG+PTINSQG
Sbjct: 302 HYEPDSGMQVTIAFNHFGEKLIQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGNPTINSQG 361

Query: 361 NRYTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSV 420
           NRYTAP +  AKEVTKRVET + +W+GWNWRS+GDILVNGAFFV SG+G E++YEKAYSV
Sbjct: 362 NRYTAPTNPFAKEVTKRVETPDGDWKGWNWRSEGDILVNGAFFVASGEGAEMRYEKAYSV 421

Query: 421 EPKSVALIDQLTWHAGPLGVGGRDNNLGMW-TTGPNGGGGFVIGSGPDYTDDMS--ESLS 480
           EPKS + I Q+T+H+G LGVGGR+NNLGMW TTG  G  G  + S  DYTD+MS   S +
Sbjct: 422 EPKSASFITQITFHSGVLGVGGRNNNLGMWTTTGSEGTSG--LDSYNDYTDEMSGAGSTN 466

Query: 481 RIGSSYFVMVLS 486
           R+  S  V +LS
Sbjct: 482 RLSFSVLVFLLS 466

BLAST of Carg10438 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 584.3 bits (1505), Expect = 9.1e-167
Identity = 289/473 (61.10%), Postives = 340/473 (71.88%), Query Frame = 0

Query: 3   TTTCIVLLALLLPSCW---PLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLW 62
           T  CI+   LL+           +A+  NL+LP QHP PE V   V              
Sbjct: 15  TCICIIWFCLLVSLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQ------------- 74

Query: 63  KEIVTCCRKVNASVTRRQLLQSLEKDESS------SCYTGNPIDDCWKCDPNWPNNRQRL 122
                  RK+N S++RRQLL   + D ++      SC TGNPIDDCW+CDPNW  NRQRL
Sbjct: 75  -------RKLNDSLSRRQLLTYQQDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRL 134

Query: 123 ADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKL 182
           ADC+IGFGQ  LGGK G+FY+VTD SD+DA NP PGTLR+AVIQP+PLWI+F ++M IKL
Sbjct: 135 ADCSIGFGQGTLGGKGGQFYLVTDSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKL 194

Query: 183 KQELIFNSYKTLDGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHY 242
           K ELI  SYKT+DGRG N+ I G GC+T+Q +S+VIIHN+HIHHC PSGNT+V SSPTH 
Sbjct: 195 KHELIIGSYKTIDGRGTNIQITGHGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHV 254

Query: 243 GYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLG 302
           G+R  SDGDGIS+  S  IW+DHCSL +C DGLID ++ ST +TISNNYFSHHDEVMLLG
Sbjct: 255 GFRGVSDGDGISVSASHHIWVDHCSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLG 314

Query: 303 HSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTIN 362
           H D Y  D GMQVTIAFNHFGE LVQRMPRCR GYIHVVNNDFT WEMYAIGGS SPTIN
Sbjct: 315 HDDRYTADKGMQVTIAFNHFGEGLVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTIN 374

Query: 363 SQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKA 422
           SQGNRYTAP D NAKEVTKRV+++E  W GWNWR++GD++VNGAFFV SG G+   Y +A
Sbjct: 375 SQGNRYTAPIDPNAKEVTKRVDSNEKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARA 434

Query: 423 YSVEPKSVALIDQLTWHAGPLG-VGGRDNNLGMWTTGPNGGGGFVIG---SGP 463
            SV+PK+ A+IDQLT +AG  G   GR+   G +    NGGG    G   SGP
Sbjct: 435 TSVQPKAAAIIDQLTVNAGVFGDPSGRNGQGGSFPGITNGGGTITRGYSKSGP 467

BLAST of Carg10438 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 558.9 bits (1439), Expect = 4.1e-159
Identity = 291/490 (59.39%), Postives = 343/490 (70.00%), Query Frame = 0

Query: 13  LLPSCWPLGAAAAALNLTLPGQHPSPELVAQEVHRFNAKFLENPKLWKEIVTCCRKVNAS 72
           LL   + L +A   LNLTLP QHPSP+ VA  V                     R VN S
Sbjct: 12  LLCLFFTLLSATKPLNLTLPHQHPSPDSVALHV--------------------IRSVNES 71

Query: 73  VTRRQL------LQSLEKDESSSCYTGNPIDDCWKC-DPNWPNNRQRLADCAIGFGQYAL 132
           + RRQL        S     SSSC TGNPIDDCW+C D +W  NRQRLADC+IGFG   L
Sbjct: 72  LARRQLSSPSSSSSSSSSSSSSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTL 131

Query: 133 GGKNGEFYIVTDDSDDDAVNPKPGTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTL 192
           GGKNG+ Y+VTD SD++  NP PGTLRY VIQ +PLWIVF +NMLI+LKQELI NSYKTL
Sbjct: 132 GGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTL 191

Query: 193 DGRGANVHIVGGGCITLQYISNVIIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGIS 252
           DGRG+ VHI G GC+TLQY+ ++IIHN+HI+ C PS           +  R +SDGDGIS
Sbjct: 192 DGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCKPSAG---------FEKRGRSDGDGIS 251

Query: 253 IFGSKDIWIDHCSLSHCKDGLIDAVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQ 312
           IFGS+ IW+DHCS+SHC DGLIDAVMGST ITISNNYF+HHDEVMLLGH D+Y PD+GMQ
Sbjct: 252 IFGSQKIWVDHCSMSHCTDGLIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAPDTGMQ 311

Query: 313 VTIAFNHFGEKLVQRMPRCRRGYIHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDR 372
           VTIAFNHFG+ LVQRMPRCRRGYIHVVNNDFT+W+MYAIGGSG+PTINSQGNRY+AP D 
Sbjct: 312 VTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDP 371

Query: 373 NAKEVTKRVET-SESEWRGWNWRSDGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALI 432
           +AKEVTKRV++  + EW  WNWR++GD++ NGAFFV SG+G+   Y KA SV+PK+ +L+
Sbjct: 372 SAKEVTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVDPKAASLV 431

Query: 433 DQLTWHAGPLGVGGRDN-----NLGMWTTGPNGGGGFVIGSGPDYTDDM------SESLS 484
           DQLT +AG  G G RD+     N      G  GGGG   GS     D M      S S S
Sbjct: 432 DQLTRNAGVFG-GPRDDQGQSGNSYSPYGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSS 471

BLAST of Carg10438 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.3e-153
Identity = 243/347 (70.03%), Postives = 286/347 (82.42%), Query Frame = 0

Query: 89  SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKP 148
           SC TGNPIDDCW+CD NW  NR+RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKP
Sbjct: 117 SCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKP 176

Query: 149 GTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNV 208
           GTLR+AVIQ +PLWIVF  +M+I+LKQELI NS+KT+D RG+NVHI  G CIT+Q+I+NV
Sbjct: 177 GTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNV 236

Query: 209 IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLID 268
           IIH +HIH C P+GN MVRSSP+H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+D
Sbjct: 237 IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVD 296

Query: 269 AVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGY 328
           AVMGST IT+SNN+F+HH+EVMLLGHSDSY  D  MQVTIA+NHFGE LVQRMPRCR GY
Sbjct: 297 AVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGY 356

Query: 329 IHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRS 388
            HVVNND+T WEMYAIGGS  PTINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS
Sbjct: 357 FHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRS 416

Query: 389 DGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLG 436
           +GD+L+NGAFF  SG G    Y +A S+  K  +++D +T  AG LG
Sbjct: 417 EGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALG 463

BLAST of Carg10438 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.3e-153
Identity = 243/347 (70.03%), Postives = 286/347 (82.42%), Query Frame = 0

Query: 89  SCYTGNPIDDCWKCDPNWPNNRQRLADCAIGFGQYALGGKNGEFYIVTDDSDDDAVNPKP 148
           SC TGNPIDDCW+CD NW  NR+RLADC IGFG+ A+GG++G FYIVTD +D+D VNPKP
Sbjct: 98  SCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKP 157

Query: 149 GTLRYAVIQPQPLWIVFPANMLIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYISNV 208
           GTLR+AVIQ +PLWIVF  +M+I+LKQELI NS+KT+D RG+NVHI  G CIT+Q+I+NV
Sbjct: 158 GTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNV 217

Query: 209 IIHNIHIHHCYPSGNTMVRSSPTHYGYRTKSDGDGISIFGSKDIWIDHCSLSHCKDGLID 268
           IIH +HIH C P+GN MVRSSP+H+G+RT +DGD +SIFGS  IWIDH SLSHC DGL+D
Sbjct: 218 IIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVD 277

Query: 269 AVMGSTGITISNNYFSHHDEVMLLGHSDSYLPDSGMQVTIAFNHFGEKLVQRMPRCRRGY 328
           AVMGST IT+SNN+F+HH+EVMLLGHSDSY  D  MQVTIA+NHFGE LVQRMPRCR GY
Sbjct: 278 AVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGY 337

Query: 329 IHVVNNDFTQWEMYAIGGSGSPTINSQGNRYTAPYDRNAKEVTKRVETSESEWRGWNWRS 388
            HVVNND+T WEMYAIGGS  PTINSQGNRY AP DR AKEVTKRVET  SEW+ WNWRS
Sbjct: 338 FHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRS 397

Query: 389 DGDILVNGAFFVTSGQGLEVKYEKAYSVEPKSVALIDQLTWHAGPLG 436
           +GD+L+NGAFF  SG G    Y +A S+  K  +++D +T  AG LG
Sbjct: 398 EGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALG 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034543.11.7e-300100.00putative pectate lyase 12 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6604393.12.5e-28396.00putative pectate lyase 12, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925720.19.4e-28395.80probable pectate lyase 12 [Cucurbita moschata] >XP_022925721.1 probable pectate ... [more]
XP_022978930.13.3e-28094.81probable pectate lyase 12 [Cucurbita maxima][more]
XP_023544235.11.3e-27995.22probable pectate lyase 12 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SCP24.3e-20673.37Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2[more]
Q93Z045.7e-15859.39Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1[more]
Q944R14.7e-15270.03Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q940Q14.9e-14967.92Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q9C5M84.6e-14760.10Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EJ044.6e-28395.80Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111433050 PE=3 SV=1[more]
A0A6J1IRN31.6e-28094.81Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478733 PE=3 SV=1[more]
A0A6J1I6R71.5e-25487.17Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111471237 PE=3 SV=1[more]
A0A0A0KIG04.4e-25487.94Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G447060 PE=3 SV=1[more]
A0A6J1HI825.8e-25487.17Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463214 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53190.13.1e-20773.37Pectin lyase-like superfamily protein [more]
AT5G04310.19.1e-16761.10Pectin lyase-like superfamily protein [more]
AT3G54920.14.1e-15959.39Pectin lyase-like superfamily protein [more]
AT4G13710.13.3e-15370.03Pectin lyase-like superfamily protein [more]
AT4G13710.23.3e-15370.03Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 176..192
score: 67.79
coord: 140..165
score: 67.22
coord: 240..261
score: 67.53
coord: 342..361
score: 72.14
coord: 413..436
score: 27.18
coord: 320..339
score: 68.1
coord: 384..408
score: 50.67
coord: 116..133
score: 57.67
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 168..365
e-value: 2.9E-93
score: 325.8
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 174..357
e-value: 2.9E-22
score: 79.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 93..435
e-value: 2.5E-117
score: 393.7
NoneNo IPR availablePANTHERPTHR31683:SF21PECTATE LYASE 12-RELATEDcoord: 2..454
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 2..454
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 88..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10438-RACarg10438-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity