Carg10400 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10400
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCarg_Chr10: 813967 .. 814758 (+)
RNA-Seq ExpressionCarg10400
SyntenyCarg10400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCAGTCCACGTCCAGGCCGCTCAAACTTGAAGGCAAAGTAGCTATTGTCACCGGCGGAGCAAGCGGCATCGGCGAGTCCACCGTACGTCTGTTCGCCGACAACGGCGTCAAAGTAGTCGTAATCGCCGATATCCAAGACGACCGAGGACGCCAAGTTGCCGAATCGATCGGCAGCCAAAGGTGTAGCTACATCCGCTGCGACGTAACGGACGAAAATCAAGTGCAATCAATGGTGGAATCAACGGTGAAAAAGTACGGAAAATTGGACGTGATGTTCAGCAACGCAGGAGTATTAAGCAGCCAAGACCAAACCATCCTGGACCTGGATCTCTCAGCCTCAGACAAACTGTTCGAGGTGAACGTGAAGGGGATGGCGGCGTGCGTGAAGCACGCGGCTAGGGCGATGGTTGAAGGGGGAGTAAGAGGGAGCATCGTGTGCACGGGGAGTGTGGCGGCAGCGAACGGGAGTAGACGGAGGACGGACTACTGTATGTCGAAGCACGCAGTGTTAGGGTTGGTGCGATCGGCGAGCGTGCAGCTTGGAGGGAAGGGAATAAGGGTGAATTGCGTGTCGCCGAGTGGGGTAGCGACGGCGGCGACATGTAGGTCGCTGGGAATGGGAATGGAGGACGTCGAGAAAGCGTACAGAGGAATGTCGAGGCTGGAAGGTGTAGCGTTGAAGGCGAGTCATGTCGCTGACGCTGTGCTCTTCCTCGCTTCCGATGACTCAGCCTTTGTCACGGGCCATGACCTTGTGGTCGACGGTAGTTTCGTCCCTGTCCTTTAA

mRNA sequence

ATGTCGCAGTCCACGTCCAGGCCGCTCAAACTTGAAGGCAAAGTAGCTATTGTCACCGGCGGAGCAAGCGGCATCGGCGAGTCCACCGTACGTCTGTTCGCCGACAACGGCGTCAAAGTAGTCGTAATCGCCGATATCCAAGACGACCGAGGACGCCAAGTTGCCGAATCGATCGGCAGCCAAAGGTGTAGCTACATCCGCTGCGACGTAACGGACGAAAATCAAGTGCAATCAATGGTGGAATCAACGGTGAAAAAGTACGGAAAATTGGACGTGATGTTCAGCAACGCAGGAGTATTAAGCAGCCAAGACCAAACCATCCTGGACCTGGATCTCTCAGCCTCAGACAAACTGTTCGAGGTGAACGTGAAGGGGATGGCGGCGTGCGTGAAGCACGCGGCTAGGGCGATGGTTGAAGGGGGAGTAAGAGGGAGCATCGTGTGCACGGGGAGTGTGGCGGCAGCGAACGGGAGTAGACGGAGGACGGACTACTGTATGTCGAAGCACGCAGTGTTAGGGTTGGTGCGATCGGCGAGCGTGCAGCTTGGAGGGAAGGGAATAAGGGTGAATTGCGTGTCGCCGAGTGGGGTAGCGACGGCGGCGACATGTAGGTCGCTGGGAATGGGAATGGAGGACGTCGAGAAAGCGTACAGAGGAATGTCGAGGCTGGAAGGTGTAGCGTTGAAGGCGAGTCATGTCGCTGACGCTGTGCTCTTCCTCGCTTCCGATGACTCAGCCTTTGTCACGGGCCATGACCTTGTGGTCGACGGTAGTTTCGTCCCTGTCCTTTAA

Coding sequence (CDS)

ATGTCGCAGTCCACGTCCAGGCCGCTCAAACTTGAAGGCAAAGTAGCTATTGTCACCGGCGGAGCAAGCGGCATCGGCGAGTCCACCGTACGTCTGTTCGCCGACAACGGCGTCAAAGTAGTCGTAATCGCCGATATCCAAGACGACCGAGGACGCCAAGTTGCCGAATCGATCGGCAGCCAAAGGTGTAGCTACATCCGCTGCGACGTAACGGACGAAAATCAAGTGCAATCAATGGTGGAATCAACGGTGAAAAAGTACGGAAAATTGGACGTGATGTTCAGCAACGCAGGAGTATTAAGCAGCCAAGACCAAACCATCCTGGACCTGGATCTCTCAGCCTCAGACAAACTGTTCGAGGTGAACGTGAAGGGGATGGCGGCGTGCGTGAAGCACGCGGCTAGGGCGATGGTTGAAGGGGGAGTAAGAGGGAGCATCGTGTGCACGGGGAGTGTGGCGGCAGCGAACGGGAGTAGACGGAGGACGGACTACTGTATGTCGAAGCACGCAGTGTTAGGGTTGGTGCGATCGGCGAGCGTGCAGCTTGGAGGGAAGGGAATAAGGGTGAATTGCGTGTCGCCGAGTGGGGTAGCGACGGCGGCGACATGTAGGTCGCTGGGAATGGGAATGGAGGACGTCGAGAAAGCGTACAGAGGAATGTCGAGGCTGGAAGGTGTAGCGTTGAAGGCGAGTCATGTCGCTGACGCTGTGCTCTTCCTCGCTTCCGATGACTCAGCCTTTGTCACGGGCCATGACCTTGTGGTCGACGGTAGTTTCGTCCCTGTCCTTTAA

Protein sequence

MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVTGHDLVVDGSFVPVL
Homology
BLAST of Carg10400 vs. NCBI nr
Match: XP_022921572.1 ((-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like [Cucurbita moschata] >KAG7023181.1 hypothetical protein SDJN02_14206, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.1 bits (1289), Expect = 5.7e-138
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 0

Query: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60
           MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS
Sbjct: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60

Query: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120
           QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE
Sbjct: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120

Query: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180
           VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV
Sbjct: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180

Query: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240
           QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL
Sbjct: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240

Query: 241 ASDDSAFVTGHDLVVDGSFVPVL 264
           ASDDSAFVTGHDLVVDGSFVPVL
Sbjct: 241 ASDDSAFVTGHDLVVDGSFVPVL 263

BLAST of Carg10400 vs. NCBI nr
Match: KAG6589496.1 (Nepetalactol-related short-chain-dehydrogenase/reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 499.6 bits (1285), Expect = 1.7e-137
Identity = 262/263 (99.62%), Postives = 262/263 (99.62%), Query Frame = 0

Query: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60
           MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS
Sbjct: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60

Query: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120
           QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE
Sbjct: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120

Query: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180
           VNVKGMAACVKHAARAMVEGGVRGSIVCTGSV AANGSRRRTDYCMSKHAVLGLVRSASV
Sbjct: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVVAANGSRRRTDYCMSKHAVLGLVRSASV 180

Query: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240
           QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL
Sbjct: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240

Query: 241 ASDDSAFVTGHDLVVDGSFVPVL 264
           ASDDSAFVTGHDLVVDGSFVPVL
Sbjct: 241 ASDDSAFVTGHDLVVDGSFVPVL 263

BLAST of Carg10400 vs. NCBI nr
Match: XP_022987198.1 ((-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 468.0 bits (1203), Expect = 5.4e-128
Identity = 246/257 (95.72%), Postives = 250/257 (97.28%), Query Frame = 0

Query: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60
           MSQSTSRPLKLE KVAIVTGGASGIGESTVRLF DNGVKVVVIADIQDDRGRQVAESIGS
Sbjct: 1   MSQSTSRPLKLEAKVAIVTGGASGIGESTVRLFVDNGVKVVVIADIQDDRGRQVAESIGS 60

Query: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120
           QRC YI CDVTDENQVQSMVESTVK+YGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE
Sbjct: 61  QRCRYILCDVTDENQVQSMVESTVKQYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120

Query: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180
           VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRR TDYCMSKHAVLGLVRSASV
Sbjct: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRGTDYCMSKHAVLGLVRSASV 180

Query: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240
           QLGGKGIRVNCVSPSGVATAATCR+LGMGME+VEKAYRGM R+EGVALKASHVA AVLFL
Sbjct: 181 QLGGKGIRVNCVSPSGVATAATCRALGMGMEEVEKAYRGMWRVEGVALKASHVAHAVLFL 240

Query: 241 ASDDSAFVTGHDLVVDG 258
           ASDDSAFVTGHDLVVDG
Sbjct: 241 ASDDSAFVTGHDLVVDG 257

BLAST of Carg10400 vs. NCBI nr
Match: CAB4311328.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 367.5 bits (942), Expect = 9.8e-98
Identity = 185/258 (71.71%), Postives = 219/258 (84.88%), Query Frame = 0

Query: 4   STSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRC 63
           ST R  KL+GK+AI+TGGASGIGE+T RLFA++G  VVVIADIQD+ GRQVAESIG+ RC
Sbjct: 2   STLRENKLQGKIAIITGGASGIGEATARLFANHGAAVVVIADIQDELGRQVAESIGTHRC 61

Query: 64  SYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNV 123
           +Y+RCDV+DE+QV++MVE TV KYG LD+MFSNAG+LSS DQ +LDLD SA D+LF+VNV
Sbjct: 62  TYVRCDVSDEDQVKTMVEQTVHKYGNLDIMFSNAGILSSPDQPVLDLDFSAFDRLFDVNV 121

Query: 124 KGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLG 183
           +GMA CVKHAAR MVE  V+GSIVCT SVAA +G   RTDYCMSKHAVLGLVRSASVQLG
Sbjct: 122 RGMALCVKHAARVMVERRVKGSIVCTASVAANHGMEIRTDYCMSKHAVLGLVRSASVQLG 181

Query: 184 GKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASD 243
             GIRVNCVSP+ +AT  TCR+ G G+E++EKAY   +RL+G+ALKA HVADAVL+LA  
Sbjct: 182 VHGIRVNCVSPNALATPLTCRAAGKGVEELEKAYEKFARLKGIALKAGHVADAVLYLACG 241

Query: 244 DSAFVTGHDLVVDGSFVP 262
           +S FVTGHDLVVDG F+P
Sbjct: 242 ESEFVTGHDLVVDGGFIP 259

BLAST of Carg10400 vs. NCBI nr
Match: CAB4280918.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 363.6 bits (932), Expect = 1.4e-96
Identity = 183/257 (71.21%), Postives = 218/257 (84.82%), Query Frame = 0

Query: 4   STSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRC 63
           ST R  KL+GK+AI+TGGASGIGE+T RLFA++G  VVVIADIQD+ GRQVAESIG+ RC
Sbjct: 2   STLRENKLQGKIAIITGGASGIGEATARLFANHGAAVVVIADIQDELGRQVAESIGTHRC 61

Query: 64  SYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNV 123
           +Y+RCDV+DE+QV++MVE TV KYG LD+MFSNAG+LSS DQ +LDLD SA D+LF+VNV
Sbjct: 62  TYVRCDVSDEDQVKTMVEQTVHKYGNLDIMFSNAGILSSPDQPVLDLDFSAFDRLFDVNV 121

Query: 124 KGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLG 183
           +GMA CVKHAAR MVE  ++GSIVCT SVAA +G   RTDYCMSKHAVLGLVRSASVQLG
Sbjct: 122 RGMALCVKHAARVMVERRMKGSIVCTASVAANHGMEIRTDYCMSKHAVLGLVRSASVQLG 181

Query: 184 GKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASD 243
             GIRVNCVSP+ +AT  TCR+ G G+E++EKAY   +RL+G+ALKA HVADAVL+LA  
Sbjct: 182 VHGIRVNCVSPNALATPLTCRAAGKGVEELEKAYEKFARLKGIALKAGHVADAVLYLACG 241

Query: 244 DSAFVTGHDLVVDGSFV 261
           +S FVTGHDLVVDG F+
Sbjct: 242 ESEFVTGHDLVVDGGFI 258

BLAST of Carg10400 vs. ExPASy Swiss-Prot
Match: A0A3Q8GYY4 ((+)-cis,trans-nepetalactol synthase NEPS2 OS=Nepeta racemosa OX=54731 GN=NEPS2 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.0e-73
Identity = 152/254 (59.84%), Postives = 190/254 (74.80%), Query Frame = 0

Query: 11  LEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCDV 70
           LEGKVAIVTGGASGIGE+  R+FA+ G + VVIADIQ + GR+VAESIG++RCSY++CD+
Sbjct: 7   LEGKVAIVTGGASGIGETAARVFANLGARAVVIADIQSELGREVAESIGAKRCSYVQCDI 66

Query: 71  TDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQD--QTILDLDLSASDKLFEVNVKGMAA 130
            DE QV+SMVE T   YG LDVMF NAG++S  +  QT+L+LD+S  D++  VN +G +A
Sbjct: 67  GDEEQVKSMVEWTATTYGALDVMFCNAGIMSKAESAQTVLELDMSKFDEVMRVNTRGTSA 126

Query: 131 CVKHAARAMVEGGVR-GSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGI 190
           CVK AAR MVE G + G+IVCT S  A+ G    TDY MSKHAV+GLVRSAS+QLG  GI
Sbjct: 127 CVKQAARKMVELGTKGGAIVCTSSPLASRGGYIDTDYVMSKHAVMGLVRSASMQLGAHGI 186

Query: 191 RVNCVSPSGVATAATCRSLGMGM-EDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSA 250
           RVN VSP  V T  T R +G+    DVE A+   + L+GVAL A HVA+A  FLASD++A
Sbjct: 187 RVNSVSPMAVLTPLT-RRMGLATPADVENAFGRFTSLKGVALTAEHVAEAAAFLASDEAA 246

Query: 251 FVTGHDLVVDGSFV 261
           F+TGHDL+VDG  +
Sbjct: 247 FITGHDLMVDGGLL 259

BLAST of Carg10400 vs. ExPASy Swiss-Prot
Match: A0A3Q8GL18 ((+)-cis,trans-nepetalactol synthase NEPS1 OS=Nepeta racemosa OX=54731 GN=NEPS1 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.3e-72
Identity = 143/262 (54.58%), Postives = 187/262 (71.37%), Query Frame = 0

Query: 4   STSRPL-----KLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESI 63
           ST+ P+     KLEGKV IVTGGASGIG++  R+FA +G + VVIADIQ + G+ VA+SI
Sbjct: 3   STANPMQVMKKKLEGKVVIVTGGASGIGQTAARVFAQHGARAVVIADIQSEVGKSVAKSI 62

Query: 64  GSQRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKL 123
           G   C Y++CDV+DE +V+SM+E T   YG LD+MFSN G++S   QT++DLDL   DK+
Sbjct: 63  GDP-CCYVQCDVSDEEEVKSMIEWTASAYGGLDMMFSNVGIMSKSAQTVMDLDLLEFDKV 122

Query: 124 FEVNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSA 183
             VN +GMAAC+KHAAR MVE G RG+I+CT +  ++ G +  TDY MSKHAV+GLVRSA
Sbjct: 123 MRVNARGMAACLKHAARKMVELGTRGTIICTTTPLSSRGGQSMTDYAMSKHAVMGLVRSA 182

Query: 184 SVQLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVL 243
           S+QLG  GIRVNCV+PS V T    R      +D    +   + L+GV L    VA+AV+
Sbjct: 183 SIQLGAHGIRVNCVTPSVVLTPLAQRMGLATPDDFHTHFGNFTSLKGVYLTPEQVAEAVV 242

Query: 244 FLASDDSAFVTGHDLVVDGSFV 261
           +LASDD+AF+TGHDLV+DG  +
Sbjct: 243 YLASDDAAFITGHDLVLDGGLL 263

BLAST of Carg10400 vs. ExPASy Swiss-Prot
Match: Q5C9I9 ((-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.7e-72
Identity = 147/248 (59.27%), Postives = 178/248 (71.77%), Query Frame = 0

Query: 10  KLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCD 69
           KL GKVAIVTGGASGIGE T RLFA+ G + VVIAD+Q ++G  VAESIG +RCSY+ CD
Sbjct: 6   KLAGKVAIVTGGASGIGEVTARLFAERGARAVVIADMQPEKGGTVAESIGGRRCSYVHCD 65

Query: 70  VTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAAC 129
           +TDE QV+S+V+ T   YG +DVMF NAG  S+  QT+LDLDL+  D++  VN +G AAC
Sbjct: 66  ITDEQQVRSVVDWTAATYGGVDVMFCNAGTASATAQTVLDLDLAQFDRVMRVNARGTAAC 125

Query: 130 VKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRV 189
           VK AAR MVE G  G+I+CT S    +     TDY MSK  VLGLVRSAS+QLG  GIRV
Sbjct: 126 VKQAARKMVELGRGGAIICTASATVHHAGPNLTDYIMSKCGVLGLVRSASLQLGVHGIRV 185

Query: 190 NCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVT 249
           N VSP+ +AT  T         DVE  Y  ++ L+GVA+ A HVA+AV FLASD++AFVT
Sbjct: 186 NSVSPTALATPLTATIGLRTAADVESFYGQVTSLKGVAITAEHVAEAVAFLASDEAAFVT 245

Query: 250 GHDLVVDG 258
           GHDL VDG
Sbjct: 246 GHDLAVDG 253

BLAST of Carg10400 vs. ExPASy Swiss-Prot
Match: A0A3Q8GLE8 ((+)-cis,cis-nepetalactol synthase NEPS3 OS=Nepeta racemosa OX=54731 GN=NEPS3 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.6e-72
Identity = 151/249 (60.64%), Postives = 177/249 (71.08%), Query Frame = 0

Query: 10  KLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCD 69
           KLEGKVAIVTGGASGIGE+T RLF   G + VVIADIQ + GR VAESIG +RCS+++CD
Sbjct: 11  KLEGKVAIVTGGASGIGEATARLFVKYGARAVVIADIQSELGRSVAESIGKERCSFVQCD 70

Query: 70  VTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAAC 129
           V DE QV+SM+E T   YG LDVMFSNAGVL+S  QT+ DLDL   DK+  VN +G A C
Sbjct: 71  VADEEQVKSMIEWTATTYGGLDVMFSNAGVLNSAAQTVKDLDLPLFDKVMRVNTRGAAVC 130

Query: 130 VKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRV 189
           VK AAR MVE G  GSI+C    +A  G+   TDY MSKHAV+GLVRSAS+QLG   IRV
Sbjct: 131 VKQAARKMVELGRGGSIICNAGSSAVRGAHGVTDYVMSKHAVIGLVRSASMQLGAHSIRV 190

Query: 190 NCVSPSGVATAATCRSLGMGM-EDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFV 249
           N VSP  VAT  T R+ G+   +DV+K       L+GV   A  VA+A  FL SD++AFV
Sbjct: 191 NSVSPMAVATPLT-RNQGISTPDDVQKFLMPFISLKGVPPTAEQVAEAAAFLGSDEAAFV 250

Query: 250 TGHDLVVDG 258
           TGHDL VDG
Sbjct: 251 TGHDLPVDG 258

BLAST of Carg10400 vs. ExPASy Swiss-Prot
Match: F4J2Z7 (Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.1e-65
Identity = 136/249 (54.62%), Postives = 178/249 (71.49%), Query Frame = 0

Query: 11  LEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCDV 70
           L+GK+AI+TGGASGIG   VRLF D+G KVV++ DIQ++ G+ +A SIG  + S+ RC+V
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIV-DIQEELGQNLAVSIGLDKASFYRCNV 103

Query: 71  TDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAACV 130
           TDE  V++ V+ TV+K+GKLDV+FSNAGVL +   ++LDLDL A D+   VNV+G AA +
Sbjct: 104 TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFI 163

Query: 131 KHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRVN 190
           KHAAR+MV  G RGSIVCT S+AA  G      Y  SKHA+LGL+RSA   LG  GIRVN
Sbjct: 164 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 223

Query: 191 CVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVTG 250
            V+P GVAT  T       ++ +E+    +  L+GV LKA H+A+A LFLASDDS +++G
Sbjct: 224 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYISG 283

Query: 251 HDLVVDGSF 260
            +LVVDG F
Sbjct: 284 QNLVVDGGF 290

BLAST of Carg10400 vs. ExPASy TrEMBL
Match: A0A6J1E0V8 ((-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111429797 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.8e-138
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 0

Query: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60
           MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS
Sbjct: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60

Query: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120
           QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE
Sbjct: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120

Query: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180
           VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV
Sbjct: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180

Query: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240
           QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL
Sbjct: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240

Query: 241 ASDDSAFVTGHDLVVDGSFVPVL 264
           ASDDSAFVTGHDLVVDGSFVPVL
Sbjct: 241 ASDDSAFVTGHDLVVDGSFVPVL 263

BLAST of Carg10400 vs. ExPASy TrEMBL
Match: A0A6J1JIS1 ((-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111484820 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.6e-128
Identity = 246/257 (95.72%), Postives = 250/257 (97.28%), Query Frame = 0

Query: 1   MSQSTSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGS 60
           MSQSTSRPLKLE KVAIVTGGASGIGESTVRLF DNGVKVVVIADIQDDRGRQVAESIGS
Sbjct: 1   MSQSTSRPLKLEAKVAIVTGGASGIGESTVRLFVDNGVKVVVIADIQDDRGRQVAESIGS 60

Query: 61  QRCSYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120
           QRC YI CDVTDENQVQSMVESTVK+YGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE
Sbjct: 61  QRCRYILCDVTDENQVQSMVESTVKQYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFE 120

Query: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASV 180
           VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRR TDYCMSKHAVLGLVRSASV
Sbjct: 121 VNVKGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRGTDYCMSKHAVLGLVRSASV 180

Query: 181 QLGGKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFL 240
           QLGGKGIRVNCVSPSGVATAATCR+LGMGME+VEKAYRGM R+EGVALKASHVA AVLFL
Sbjct: 181 QLGGKGIRVNCVSPSGVATAATCRALGMGMEEVEKAYRGMWRVEGVALKASHVAHAVLFL 240

Query: 241 ASDDSAFVTGHDLVVDG 258
           ASDDSAFVTGHDLVVDG
Sbjct: 241 ASDDSAFVTGHDLVVDG 257

BLAST of Carg10400 vs. ExPASy TrEMBL
Match: A0A6J5XJX2 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS33463 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.8e-98
Identity = 185/258 (71.71%), Postives = 219/258 (84.88%), Query Frame = 0

Query: 4   STSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRC 63
           ST R  KL+GK+AI+TGGASGIGE+T RLFA++G  VVVIADIQD+ GRQVAESIG+ RC
Sbjct: 2   STLRENKLQGKIAIITGGASGIGEATARLFANHGAAVVVIADIQDELGRQVAESIGTHRC 61

Query: 64  SYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNV 123
           +Y+RCDV+DE+QV++MVE TV KYG LD+MFSNAG+LSS DQ +LDLD SA D+LF+VNV
Sbjct: 62  TYVRCDVSDEDQVKTMVEQTVHKYGNLDIMFSNAGILSSPDQPVLDLDFSAFDRLFDVNV 121

Query: 124 KGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLG 183
           +GMA CVKHAAR MVE  V+GSIVCT SVAA +G   RTDYCMSKHAVLGLVRSASVQLG
Sbjct: 122 RGMALCVKHAARVMVERRVKGSIVCTASVAANHGMEIRTDYCMSKHAVLGLVRSASVQLG 181

Query: 184 GKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASD 243
             GIRVNCVSP+ +AT  TCR+ G G+E++EKAY   +RL+G+ALKA HVADAVL+LA  
Sbjct: 182 VHGIRVNCVSPNALATPLTCRAAGKGVEELEKAYEKFARLKGIALKAGHVADAVLYLACG 241

Query: 244 DSAFVTGHDLVVDGSFVP 262
           +S FVTGHDLVVDG F+P
Sbjct: 242 ESEFVTGHDLVVDGGFIP 259

BLAST of Carg10400 vs. ExPASy TrEMBL
Match: A0A6J5V4H4 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS33876 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 6.9e-97
Identity = 183/257 (71.21%), Postives = 218/257 (84.82%), Query Frame = 0

Query: 4   STSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRC 63
           ST R  KL+GK+AI+TGGASGIGE+T RLFA++G  VVVIADIQD+ GRQVAESIG+ RC
Sbjct: 2   STLRENKLQGKIAIITGGASGIGEATARLFANHGAAVVVIADIQDELGRQVAESIGTHRC 61

Query: 64  SYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNV 123
           +Y+RCDV+DE+QV++MVE TV KYG LD+MFSNAG+LSS DQ +LDLD SA D+LF+VNV
Sbjct: 62  TYVRCDVSDEDQVKTMVEQTVHKYGNLDIMFSNAGILSSPDQPVLDLDFSAFDRLFDVNV 121

Query: 124 KGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLG 183
           +GMA CVKHAAR MVE  ++GSIVCT SVAA +G   RTDYCMSKHAVLGLVRSASVQLG
Sbjct: 122 RGMALCVKHAARVMVERRMKGSIVCTASVAANHGMEIRTDYCMSKHAVLGLVRSASVQLG 181

Query: 184 GKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASD 243
             GIRVNCVSP+ +AT  TCR+ G G+E++EKAY   +RL+G+ALKA HVADAVL+LA  
Sbjct: 182 VHGIRVNCVSPNALATPLTCRAAGKGVEELEKAYEKFARLKGIALKAGHVADAVLYLACG 241

Query: 244 DSAFVTGHDLVVDGSFV 261
           +S FVTGHDLVVDG F+
Sbjct: 242 ESEFVTGHDLVVDGGFI 258

BLAST of Carg10400 vs. ExPASy TrEMBL
Match: M5WBK1 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G185700 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.2e-96
Identity = 183/256 (71.48%), Postives = 217/256 (84.77%), Query Frame = 0

Query: 4   STSRPLKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRC 63
           ST R  KL+GK+AI+TGGASGIGE+T RLFA++G  VVVIADIQD+ GRQVAESIG+ RC
Sbjct: 2   STLRENKLQGKIAIITGGASGIGEATARLFANHGAAVVVIADIQDELGRQVAESIGTHRC 61

Query: 64  SYIRCDVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNV 123
           +Y+ CDV+DE+QV++MVE TV+KYG LD+MFSNAG+LSS DQT+LDLD SA D+LF+VNV
Sbjct: 62  TYVHCDVSDEDQVKTMVEQTVRKYGHLDIMFSNAGILSSPDQTVLDLDFSAFDRLFDVNV 121

Query: 124 KGMAACVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLG 183
           +GMA CVKHAAR MVE  V+GSIVCT SVAA +G   RTDYCMSKHAVLGLVRSASVQLG
Sbjct: 122 RGMALCVKHAARVMVERRVKGSIVCTASVAANHGMAIRTDYCMSKHAVLGLVRSASVQLG 181

Query: 184 GKGIRVNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASD 243
             GIRVNCVSP+ +AT  TCR+ G G+E+VEK Y+  +RL+G+ LKA HVADAVL+LA  
Sbjct: 182 VHGIRVNCVSPTALATPLTCRAAGKGVEEVEKEYKKFARLKGIVLKAGHVADAVLYLACG 241

Query: 244 DSAFVTGHDLVVDGSF 260
           +S FVTGHDLVVDG F
Sbjct: 242 ESEFVTGHDLVVDGGF 257

BLAST of Carg10400 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 7.3e-67
Identity = 137/251 (54.58%), Postives = 180/251 (71.71%), Query Frame = 0

Query: 9   LKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRC 68
           L+L+GK+AI+TGGASGIG   VRLF D+G KVV++ DIQ++ G+ +A SIG  + S+ RC
Sbjct: 4   LRLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIV-DIQEELGQNLAVSIGLDKASFYRC 63

Query: 69  DVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAA 128
           +VTDE  V++ V+ TV+K+GKLDV+FSNAGVL +   ++LDLDL A D+   VNV+G AA
Sbjct: 64  NVTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAA 123

Query: 129 CVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIR 188
            +KHAAR+MV  G RGSIVCT S+AA  G      Y  SKHA+LGL+RSA   LG  GIR
Sbjct: 124 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 183

Query: 189 VNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFV 248
           VN V+P GVAT  T       ++ +E+    +  L+GV LKA H+A+A LFLASDDS ++
Sbjct: 184 VNGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYI 243

Query: 249 TGHDLVVDGSF 260
           +G +LVVDG F
Sbjct: 244 SGQNLVVDGGF 252

BLAST of Carg10400 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 3.6e-66
Identity = 136/249 (54.62%), Postives = 178/249 (71.49%), Query Frame = 0

Query: 11  LEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCDV 70
           L+GK+AI+TGGASGIG   VRLF D+G KVV++ DIQ++ G+ +A SIG  + S+ RC+V
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIV-DIQEELGQNLAVSIGLDKASFYRCNV 103

Query: 71  TDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAACV 130
           TDE  V++ V+ TV+K+GKLDV+FSNAGVL +   ++LDLDL A D+   VNV+G AA +
Sbjct: 104 TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFI 163

Query: 131 KHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRVN 190
           KHAAR+MV  G RGSIVCT S+AA  G      Y  SKHA+LGL+RSA   LG  GIRVN
Sbjct: 164 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 223

Query: 191 CVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVTG 250
            V+P GVAT  T       ++ +E+    +  L+GV LKA H+A+A LFLASDDS +++G
Sbjct: 224 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYISG 283

Query: 251 HDLVVDGSF 260
            +LVVDG F
Sbjct: 284 QNLVVDGGF 290

BLAST of Carg10400 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.1e-65
Identity = 133/250 (53.20%), Postives = 178/250 (71.20%), Query Frame = 0

Query: 10  KLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCD 69
           +L+GK+ I+TGGASGIG  +VRLF ++G +VV++ D+QD+ G+ VA SIG  + SY  CD
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIV-DVQDELGQNVAVSIGEDKASYYHCD 64

Query: 70  VTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAAC 129
           VT+E +V++ V+ TV+KYGKLDV+FSNAGV+     +ILDL+L+  D+   +N++G AA 
Sbjct: 65  VTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTAAF 124

Query: 130 VKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRV 189
           +KHAARAMVE G+RGSIVCT SVAA         Y  SKH +LGL++SAS  LG  GIRV
Sbjct: 125 IKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRV 184

Query: 190 NCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVT 249
           N V+P GVAT   C    M    VE+     + L+G+ LKA HVA+A LFLASD+SA+V+
Sbjct: 185 NGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYVS 244

Query: 250 GHDLVVDGSF 260
           G +L VDG +
Sbjct: 245 GQNLAVDGGY 252

BLAST of Carg10400 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.5e-64
Identity = 139/251 (55.38%), Postives = 177/251 (70.52%), Query Frame = 0

Query: 9   LKLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRC 68
           L+L+GK+AI+TGGASGIG   VRLF D+G KVV++ D Q++ G+ VA S+G  + S+ RC
Sbjct: 4   LRLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIV-DFQEELGQNVAVSVGKDKASFYRC 63

Query: 69  DVTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAA 128
           DVT+E +V++ V+ TV+KYGKLDV+FSNAGV+  Q  + LDL+L   D+   VNV+G AA
Sbjct: 64  DVTNEKEVENAVKFTVEKYGKLDVLFSNAGVM-EQPGSFLDLNLEQFDRTMAVNVRGAAA 123

Query: 129 CVKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIR 188
            +KHAARAMVE G RGSIVCT SVA+  G      Y  SKHA+LGLV+SA   LG  GIR
Sbjct: 124 FIKHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIR 183

Query: 189 VNCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFV 248
           VN V+P  VATA   R     +  VE+       L+GV LKA HVA+A LFLASDDSA+V
Sbjct: 184 VNGVAPYAVATAINSRD-EETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDSAYV 243

Query: 249 TGHDLVVDGSF 260
           +G +L VDG +
Sbjct: 244 SGQNLAVDGGY 251

BLAST of Carg10400 vs. TAIR 10
Match: AT3G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.3e-63
Identity = 136/250 (54.40%), Postives = 175/250 (70.00%), Query Frame = 0

Query: 10  KLEGKVAIVTGGASGIGESTVRLFADNGVKVVVIADIQDDRGRQVAESIGSQRCSYIRCD 69
           +L+GK+ I+TGGASGIG    RLF D+G KVV++ D+Q++ G+ VA SIG  + S+ RCD
Sbjct: 5   RLDGKIVIITGGASGIGAEAARLFTDHGAKVVIV-DLQEELGQNVAVSIGLDKASFYRCD 64

Query: 70  VTDENQVQSMVESTVKKYGKLDVMFSNAGVLSSQDQTILDLDLSASDKLFEVNVKGMAAC 129
           +TDE +V++ V+ TV+K+GKLDV+FSNAGV+     +ILDLDL A D+   VNV+G AA 
Sbjct: 65  ITDETEVENAVKFTVEKHGKLDVLFSNAGVMEPHG-SILDLDLEAFDRTMAVNVRGAAAF 124

Query: 130 VKHAARAMVEGGVRGSIVCTGSVAAANGSRRRTDYCMSKHAVLGLVRSASVQLGGKGIRV 189
           +KHAAR+MV  G RGSIVCT SV A  G      Y  SKHA+LGLVRSA   LG  GIRV
Sbjct: 125 IKHAARSMVASGTRGSIVCTTSVTAEIGGPGPHSYTASKHALLGLVRSACGGLGKYGIRV 184

Query: 190 NCVSPSGVATAATCRSLGMGMEDVEKAYRGMSRLEGVALKASHVADAVLFLASDDSAFVT 249
           N V+P GVAT  T  +    ++ VE      + L+GV LKA HVADA LFLASDDS +++
Sbjct: 185 NGVAPYGVATGLTSYN-EETVKMVEDYCSATAILKGVVLKARHVADAALFLASDDSVYIS 244

Query: 250 GHDLVVDGSF 260
           G +L VDG +
Sbjct: 245 GQNLGVDGGY 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022921572.15.7e-138100.00(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like [Cucurbita mosc... [more]
KAG6589496.11.7e-13799.62Nepetalactol-related short-chain-dehydrogenase/reductase 1, partial [Cucurbita a... [more]
XP_022987198.15.4e-12895.72(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like [Cucurbita maxi... [more]
CAB4311328.19.8e-9871.71unnamed protein product [Prunus armeniaca][more]
CAB4280918.11.4e-9671.21unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
A0A3Q8GYY41.0e-7359.84(+)-cis,trans-nepetalactol synthase NEPS2 OS=Nepeta racemosa OX=54731 GN=NEPS2 P... [more]
A0A3Q8GL183.3e-7254.58(+)-cis,trans-nepetalactol synthase NEPS1 OS=Nepeta racemosa OX=54731 GN=NEPS1 P... [more]
Q5C9I95.7e-7259.27(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial OS=Mentha piperita O... [more]
A0A3Q8GLE89.6e-7260.64(+)-cis,cis-nepetalactol synthase NEPS3 OS=Nepeta racemosa OX=54731 GN=NEPS3 PE=... [more]
F4J2Z75.1e-6554.62Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1E0V82.8e-138100.00(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like OS=Cucurbita mo... [more]
A0A6J1JIS12.6e-12895.72(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial-like OS=Cucurbita ma... [more]
A0A6J5XJX24.8e-9871.71Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS33463 PE=... [more]
A0A6J5V4H46.9e-9771.21Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS33876 PE=4 S... [more]
M5WBK11.2e-9671.48Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G185700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G29250.27.3e-6754.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.13.6e-6654.62NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.11.1e-6553.20NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47130.11.5e-6455.38NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29260.11.3e-6354.40NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 88..99
score: 40.2
coord: 144..152
score: 45.26
coord: 164..183
score: 30.53
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 88..99
score: 49.32
coord: 164..183
score: 33.32
coord: 223..243
score: 32.2
coord: 15..32
score: 48.84
coord: 138..154
score: 30.9
coord: 185..202
score: 41.74
NoneNo IPR availableSMARTSM00822PKS_KRcoord: 14..197
e-value: 0.0025
score: -53.2
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 20..257
e-value: 2.6E-60
score: 203.9
NoneNo IPR availableGENE3D3.40.50.720coord: 2..261
e-value: 2.3E-79
score: 268.3
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 11..259
e-value: 2.1E-14
score: 51.2
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 17..203
e-value: 2.3E-15
score: 54.4
NoneNo IPR availablePANTHERPTHR42820SHORT-CHAIN DEHYDROGENASE REDUCTASEcoord: 3..259
NoneNo IPR availablePANTHERPTHR42820:SF21SHORT-CHAIN DEHYDROGENASE REDUCTASE 3B-LIKEcoord: 3..259
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 151..179
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 12..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10400-RACarg10400-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity