Carg10371 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10371
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionalpha-1,2-Mannosidase
LocationCarg_Chr10: 658190 .. 663358 (-)
RNA-Seq ExpressionCarg10371
SyntenyCarg10371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATAGTTAACCAAATTATTCAATCCACGACAACCAAGTTTCTATGGGCGTCTTGATTTGAGCTGCGATTCGCTTCAGATCCCCAGCGCCATTGACTTCGTACCCCGATTCGATTCTATTCGATTCGCTTCTTCTGCCCGGAATTCTTATCCAGTCCATAGATTCCATACATGTTGCCCCGCAGGTCTTTCACATGGATTCTTCTGCTTTTCATCATCTTCTCCGCTCTCTTCAATCCTTCCAGCTCCAACAACTACTGGACTGCCAAGCGAATGCGTATGAGGGAAAAGGTCCGCCGGATGTAAGCCTCTTATCTTCATAATCTCCGTTGGCATTTTTAATTCAATCGCGGTAGTTTCGTTTTCTGTTCCGGTCTATTGGATGCGGTTCTGACTTCATTATCGTTCTGTGGATCGGTGTTGTGTTGAGAAGCTCTAGCGTTTTGTGGTATAGGAGATAATTTGTTCAATTCTACGAAAATTCTAACTATAAAGTCTAATTTTGTCATGTCTGGAGAATTTTCAGTTTTTGTTTAGTTTGTTATGATGATGACTTAGACGTTTCTATGGAGCTCGTCGTTCTTATCTGCATTTAGTCTTTTTTTTCTGGAAATTGAACGCAGTACAGTGATGATATATTGTTGGGATCTTATTCAATCTAGGTCTTCAGGAAAATTATGACTAGATTTGTTGAACTTCTATCCGAGCCACATTGAGTGCATTATTTTGACCTTTAGTTACTGTGTCTTACTATTTATTCTTGGTGAGTTGCATAATCTTAATTTAGTACACACCATTGTTCTTGCCTAAATTTCATCGGTTGTATTTGTTTAGATGATCCCTACGTTCACTAGCACGCAAAGTATCTGACAGTTGGCTTATTTTTATATGCTTTCAGGTTTTACCATGCATATGACAACTATATGACATATGCATTTCCAGTAAGTATTCTTGGTTACAGATTCATGTCTCTGATCACATAGAAACCTATTGACTGTCCTATTTCTATCAGCACGATGAGCTAAAACCTCTTACGAAGACCTTCACCAACTCCCTCAGTGAGCTCGGTAATCTTAAGGTAATCAATATAATGTTAATATGGCTTTAGAGTGGTAATTGGTTGTGGGGAGTGCATTAACTGTGTAATTCTTCAATTTTCAGCTGGAACACTTGCCACAAAATTATAATGGATCTGCTCTTACACTCATCGAATCATTGTCTAGGTATGAATTGATTACTGCCTGAATTTATGGTTGTTTAGTTATTAGTTTCATAACGATCACATTTTTCTTTTTCAGCCTTGTAATTTTAGGCAACAACACTGAATTTCAAAAGGCGGTGCTTTGGCTTTCTGAAAATTTACGTTTTGATGTTGATGCACGGATAAATCTATTTGAGGTACCCAAGTCCTTAGATATCCCCAATCCCCAAAAAGATGAATGTGGTTGGCTCATCTCTAGAGTTCAAATGCTTGTCATGCTAAGAAAGAGCTAAAATGTTGTGGATGAAGGAGCAATATATGCATTTTTACTCTTGACCACAATTTTGTAGTGCAACATAAGAGTTCTTGGAGGACTTGTTTCTGCCCATGTTCTTGCAACTGATTCCACGAACCGATTGCCTAGACAAAGTTATAAGAATCAACTACTAGTTTTGGCCGAAGACTTAGGGAACCGCTTCCTACCTGCATTTAATACCCCCACAGGGTTGCCATATGCGTGGATTAACCTCAAGGTATTACCTATCATCTGCTTAGGTCCAAATCTAATTGTTTATAAATATTATGGTATATTAAACTCCATGCTATATGATCTTCATGGGATTTTGTATTCACTGGACCCTGTCTTCTTTTATTGAGCTAATTTTGCAGTCATTGAGAACTGAGATATGTCTCTGATGCCTTATAAGTCGTTTATGCTGTTTGCATATTGAACAATATTCTTGGTATAAGTTTCTAAATGATCATAACGAGAATAAGTGAATCTCCTTAGGTGTACTGAAGAAATTTTGCATCCAACAAGCCAGATCTTACCTTGCTCTGTTCCTTTTGTTCTTCTCAGCAGTTCTTTTTTTCTTGGGGCGTGGGTGGGGTGGAGGTCAACTATTTGAATGATATTTTTAATGCAGCTTAAATTCACTGTATGCATGGTACGCTTAGGGAGTCCGTAGATATTTTCAGAAGTTTTCTTTGCAGTTTTAAAATATATGAATACGTTTTTCTGGGTCATGGTTCCATTTTCTCCCTCTAAAGAGGAGTATATGACTCCACATCAATCCAAAATTCTGTGGGCAGCATGGAGTGATGGAGAATGAGACTACTGAAACAAGTTCTTCCGGGTGTGGTGAGCTCTTATCTGTTATTGTTAAGATAAGTATACGTGAAAATTTTATGGTCTGAATTGGATCAAAATTCTCGAAATGGTGACCCCAGGTTCTCTAATTCTCGAAATGGGAGCATTATCGCGATTGACTGGTGACCCCAGGTTTGAATCTGCTGCTTTGCGTGCTCTTCGTAAGTTGTGGAGCATGCGCAGTTCATTGAATTTACTTGGAACTACATTGGATGTGGAAACAGGTGAATGGATTGAGTTCTCATCTGGAATTGGTGCTGGTAAGTCATTTGTTATTGACAATACAGATTTTTACATGTTTTGCTAGAAGTTATCACATCTTCATTTTTACTATATTTTTCTTCTTCGATAATTGTCTCCCACCTCTTATTAGGCGCTTCCATACTCTTGTAATATTAACTCAACGTCTATCAGGAAGAAAACCTGTTTTATGCAATTGAGCTCAGGGTAGAATTATACATAATTATGCTCTTGGAAGAGTAGAAACCTAATACTGATGTTGGATTCTTGTGGTTTCTTCAAGGGGTTGATTCATTCTATGAATATCTATTTAAGGCTCATGTACTTTTTGGAAAAGAAGAATTCTGGAGAATGTTCCACAGTGCTTACCTTGCTGTGCAGAAATATTTCAGACATGGTCCATGGTAGCGTGTACACTATTTTTATGATCATTTTAGTACGAACACATTTTCTTAAATTATACAAAACTATATTCATTTTTAGCCGTTTCTAAGCTACAACAATTGTAATTTTCAATGCACACCAACTTTTTTTTTTTCTCCAAATAGATTTTAAACAATAACCAATAAAAACAATTCTTTAAGAACAAATTTTTAAAAGGTATATACAAAAAATTATTATCTTATTTTGGGCGGCTTACTTGAAATTATTTGGTAATGTAGGTATCATGAGGCCGATATGAGGACAGGGAGGGCAACCCACTGGCAGCTCACAAGCCTTCAAGCATTTTGGCCTGGCCTACAGGCTAGTTGCAGTAATCTTAGAAGTTCTAGTAAATGATACTCTACTTGAATATGACTGTTTGTCCTTGACTCTCTGTTAGTAGGTTCTTGTTGGGGATATTCCGGCAGCTAATTCATCACATCGTGAGTTCTTTCATGTGTGGGAGAAATATGGAGTTCTACCGGAAAGGTATGCTGCTTGCAAATCTGTCCATTTAAACTATTCCATGTTTTGACCAGAACACAGAACTTTCGTTGTCAATTGTCTTATTTTCTTTTTGTCAATTATCTTGTTTCAATCTATTAGAGTTCCAGTATTTTCCATGACAACCATTAATTCTTAAGTCTGGGATTTTAAATTGTTCAGGTATTTACTTGACTATCAGATGCTGCATCCCACTGAAAAGTATTATCCTCTCCGTCCAGAATTGGCAGAGTCAACTTTTTACTTGTATCAAGCAACCAAAGGTTTTATTTTTCAAAAACTTCAACGTTATATATTATGATTATTTCGTTTCTCGTCGACTAAAAGTTTGAAGCTCTGCTGTAAAATAGTATTCTTGGAATGCTAGTTTCCTTATGTCTTATCCAACTATTAGTTATAAAGCTTAACTGCCTTACTCGGATTCCTTCCGTTTGGCTTTTGGAATTGTAGATCCATGGTACTTTGAAGTGGGTGAATCAATCATGAAGTCTCTTATCTTGTACACTAAAGTTGAAGGAGGATTTGCAAGTGTCAGAGATGTTACAACTATGCAGTTGGAAGATCATCAACATAGTTTTTTCCTCTCTGAAACGTATGATCTCTTTCTCTCTCTCTCTCTCCCAATTCTGTTATCCAGAAAATCAGCTTGGTCATCACACCATCCTTCTTTATGTAGGTGCAAATATTTATATCTTTTATTTGACGACTCGTTTCTGGTCGATCAAAATTACATCTTCACCACCGAGGGTCACCCCTTGCCAGTCCTAAGTTCTTGGCATGAACGACTGCCAGAGGTGTATGGCCTGACCAACGGGACATCTATCAAGGTATTACTCCCACTGTCTAGTCTTGATTTTCATTTACCCCCTCATCATAGGGAGCCTGGCAGCTGTTTGAAATATTATGCCCAATAGAGCGGGAAAATTTAATGAAGAAAAAACTTGGAAGGAATATTTTAAAATGTGAAATGAATTGGTGGAGATGAAATGCATTTATATCTGCAGGGTGAAAATTCATCAAGAAGGTCTAGTGCAATGTCTCTTCAAGTCTGCCCAGCTACCTCACTAAACACAGCTGGAGATGGTGACCAACAACTAGAGAGTGCTTGCCATATCCTTGATGCTCGAGCAGATCACAAGTGTTTCAGCGATGAAGAATGTGGAGTCGATTCGACTACTTGCAGACGGCGATCGTGTAGCTCATCTGGTTATTGTGGTCATTGGTTGCGCTTATAACACAGACGGTAATTATTTTCAATAAGATTCCAAACTTGGTTCATCTTGCACCAACCGACGACACCGAGCCGAAAATGAACTTTGTAAGTAGTTGTTAAGTCCACACGAGATAACTTTTTTTCACCCCCAAAAAAGAAAAATTCTTGAATGTAGCTACTACAAAACGATATAGATATTATTATCTTAGTTGTTAGATGCAGGGATAGTCTAAAGTTGAATTTTTAGCGGGAGGGGATATGAACATCATGTTGTTCGTTGACTTGTATGAATGGCTTCCGTGATTGGAACTAAAAGCCTTGGTTTAGAGCCGGGGGTAATTATGTCATTCTAAGCCTGAGAGTCGTGGCTTGAAAGTACAAAGGAAGATTCGTGGAAATGAGGCTGTCAACTTTAGTTGAGATTCACGCTCCTTTTT

mRNA sequence

AAATATAGTTAACCAAATTATTCAATCCACGACAACCAAGTTTCTATGGGCGTCTTGATTTGAGCTGCGATTCGCTTCAGATCCCCAGCGCCATTGACTTCGTACCCCGATTCGATTCTATTCGATTCGCTTCTTCTGCCCGGAATTCTTATCCAGTCCATAGATTCCATACATGTTGCCCCGCAGGTCTTTCACATGGATTCTTCTGCTTTTCATCATCTTCTCCGCTCTCTTCAATCCTTCCAGCTCCAACAACTACTGGACTGCCAAGCGAATGCGTATGAGGGAAAAGGTCCGCCGGATGTTTTACCATGCATATGACAACTATATGACATATGCATTTCCACACGATGAGCTAAAACCTCTTACGAAGACCTTCACCAACTCCCTCAGTGAGCTCGGTAATCTTAAGCTGGAACACTTGCCACAAAATTATAATGGATCTGCTCTTACACTCATCGAATCATTGTCTAGCCTTGTAATTTTAGGCAACAACACTGAATTTCAAAAGGCGGTGCTTTGGCTTTCTGAAAATTTACGTTTTGATGTTGATGCACGGATAAATCTATTTGAGTGCAACATAAGAGTTCTTGGAGGACTTGTTTCTGCCCATGTTCTTGCAACTGATTCCACGAACCGATTGCCTAGACAAAGTTATAAGAATCAACTACTAGTTTTGGCCGAAGACTTAGGGAACCGCTTCCTACCTGCATTTAATACCCCCACAGGGTTGCCATATGCGTGGATTAACCTCAAGCATGGAGTGATGGAGAATGAGACTACTGAAACAAGTTCTTCCGGGTGTGGTTCTCTAATTCTCGAAATGGGAGCATTATCGCGATTGACTGGTGACCCCAGGTTTGAATCTGCTGCTTTGCGTGCTCTTCGTAAGTTGTGGAGCATGCGCAGTTCATTGAATTTACTTGGAACTACATTGGATGTGGAAACAGGTGAATGGATTGAGTTCTCATCTGGAATTGGTGCTGGGGTTGATTCATTCTATGAATATCTATTTAAGGCTCATGTACTTTTTGGAAAAGAAGAATTCTGGAGAATGTTCCACAGTGCTTACCTTGCTGTGCAGAAATATTTCAGACATGGTCCATGGTATCATGAGGCCGATATGAGGACAGGGAGGGCAACCCACTGGCAGCTCACAAGCCTTCAAGCATTTTGGCCTGGCCTACAGGTTCTTGTTGGGGATATTCCGGCAGCTAATTCATCACATCGTGAGTTCTTTCATGTGTGGGAGAAATATGGAGTTCTACCGGAAAGGTATTTACTTGACTATCAGATGCTGCATCCCACTGAAAAGTATTATCCTCTCCGTCCAGAATTGGCAGAGTCAACTTTTTACTTGTATCAAGCAACCAAAGATCCATGGTACTTTGAAGTGGGTGAATCAATCATGAAGTCTCTTATCTTGTACACTAAAGTTGAAGGAGGATTTGCAAGTGTCAGAGATGTTACAACTATGCAGTTGGAAGATCATCAACATAGTTTTTTCCTCTCTGAAACGTGCAAATATTTATATCTTTTATTTGACGACTCGTTTCTGGTCGATCAAAATTACATCTTCACCACCGAGGGTCACCCCTTGCCAGTCCTAAGTTCTTGGCATGAACGACTGCCAGAGGTGTATGGCCTGACCAACGGGACATCTATCAAGGGTGAAAATTCATCAAGAAGGTCTAGTGCAATGTCTCTTCAAGTCTGCCCAGCTACCTCACTAAACACAGCTGGAGATGGTGACCAACAACTAGAGAGTGCTTGCCATATCCTTGATGCTCGAGCAGATCACAAGTGTTTCAGCGATGAAGAATGTGGAGTCGATTCGACTACTTGCAGACGGCGATCGTGTAGCTCATCTGGTTATTGTGGTCATTGGTTGCGCTTATAACACAGACGGTAATTATTTTCAATAAGATTCCAAACTTGGTTCATCTTGCACCAACCGACGACACCGAGCCGAAAATGAACTTTGTAAGTAGTTGTTAAGTCCACACGAGATAACTTTTTTTCACCCCCAAAAAAGAAAAATTCTTGAATGTAGCTACTACAAAACGATATAGATATTATTATCTTAGTTGTTAGATGCAGGGATAGTCTAAAGTTGAATTTTTAGCGGGAGGGGATATGAACATCATGTTGTTCGTTGACTTGTATGAATGGCTTCCGTGATTGGAACTAAAAGCCTTGGTTTAGAGCCGGGGGTAATTATGTCATTCTAAGCCTGAGAGTCGTGGCTTGAAAGTACAAAGGAAGATTCGTGGAAATGAGGCTGTCAACTTTAGTTGAGATTCACGCTCCTTTTT

Coding sequence (CDS)

ATGTTGCCCCGCAGGTCTTTCACATGGATTCTTCTGCTTTTCATCATCTTCTCCGCTCTCTTCAATCCTTCCAGCTCCAACAACTACTGGACTGCCAAGCGAATGCGTATGAGGGAAAAGGTCCGCCGGATGTTTTACCATGCATATGACAACTATATGACATATGCATTTCCACACGATGAGCTAAAACCTCTTACGAAGACCTTCACCAACTCCCTCAGTGAGCTCGGTAATCTTAAGCTGGAACACTTGCCACAAAATTATAATGGATCTGCTCTTACACTCATCGAATCATTGTCTAGCCTTGTAATTTTAGGCAACAACACTGAATTTCAAAAGGCGGTGCTTTGGCTTTCTGAAAATTTACGTTTTGATGTTGATGCACGGATAAATCTATTTGAGTGCAACATAAGAGTTCTTGGAGGACTTGTTTCTGCCCATGTTCTTGCAACTGATTCCACGAACCGATTGCCTAGACAAAGTTATAAGAATCAACTACTAGTTTTGGCCGAAGACTTAGGGAACCGCTTCCTACCTGCATTTAATACCCCCACAGGGTTGCCATATGCGTGGATTAACCTCAAGCATGGAGTGATGGAGAATGAGACTACTGAAACAAGTTCTTCCGGGTGTGGTTCTCTAATTCTCGAAATGGGAGCATTATCGCGATTGACTGGTGACCCCAGGTTTGAATCTGCTGCTTTGCGTGCTCTTCGTAAGTTGTGGAGCATGCGCAGTTCATTGAATTTACTTGGAACTACATTGGATGTGGAAACAGGTGAATGGATTGAGTTCTCATCTGGAATTGGTGCTGGGGTTGATTCATTCTATGAATATCTATTTAAGGCTCATGTACTTTTTGGAAAAGAAGAATTCTGGAGAATGTTCCACAGTGCTTACCTTGCTGTGCAGAAATATTTCAGACATGGTCCATGGTATCATGAGGCCGATATGAGGACAGGGAGGGCAACCCACTGGCAGCTCACAAGCCTTCAAGCATTTTGGCCTGGCCTACAGGTTCTTGTTGGGGATATTCCGGCAGCTAATTCATCACATCGTGAGTTCTTTCATGTGTGGGAGAAATATGGAGTTCTACCGGAAAGGTATTTACTTGACTATCAGATGCTGCATCCCACTGAAAAGTATTATCCTCTCCGTCCAGAATTGGCAGAGTCAACTTTTTACTTGTATCAAGCAACCAAAGATCCATGGTACTTTGAAGTGGGTGAATCAATCATGAAGTCTCTTATCTTGTACACTAAAGTTGAAGGAGGATTTGCAAGTGTCAGAGATGTTACAACTATGCAGTTGGAAGATCATCAACATAGTTTTTTCCTCTCTGAAACGTGCAAATATTTATATCTTTTATTTGACGACTCGTTTCTGGTCGATCAAAATTACATCTTCACCACCGAGGGTCACCCCTTGCCAGTCCTAAGTTCTTGGCATGAACGACTGCCAGAGGTGTATGGCCTGACCAACGGGACATCTATCAAGGGTGAAAATTCATCAAGAAGGTCTAGTGCAATGTCTCTTCAAGTCTGCCCAGCTACCTCACTAAACACAGCTGGAGATGGTGACCAACAACTAGAGAGTGCTTGCCATATCCTTGATGCTCGAGCAGATCACAAGTGTTTCAGCGATGAAGAATGTGGAGTCGATTCGACTACTTGCAGACGGCGATCGTGTAGCTCATCTGGTTATTGTGGTCATTGGTTGCGCTTATAA

Protein sequence

MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPAFNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAYLAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL
Homology
BLAST of Carg10371 vs. NCBI nr
Match: XP_023516302.1 (alpha-mannosidase I MNS5 [Cucurbita pepo subsp. pepo] >KAG7023152.1 Alpha-mannosidase I MNS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of Carg10371 vs. NCBI nr
Match: XP_022921842.1 (alpha-mannosidase I MNS5 [Cucurbita moschata] >KAG6589467.1 Alpha-mannosidase I MNS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 574/575 (99.83%), Postives = 574/575 (99.83%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NLRFDVDARINLFECNIRVLGGLVSAHVLATDST RLPRQSYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTTRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of Carg10371 vs. NCBI nr
Match: XP_022988359.1 (alpha-mannosidase I MNS5 [Cucurbita maxima])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPR+SYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRESYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALS+LTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSQLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFS+EECGVDSTTCRRRSCSSSGYCGHWLRL
Sbjct: 541 ADHKCFSNEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of Carg10371 vs. NCBI nr
Match: XP_038879418.1 (alpha-mannosidase I MNS5 [Benincasa hispida])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 553/573 (96.51%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSS+NYWTAKRMRMREKVRRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSSNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL RQ+YKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVME+ETTETS+SGCGSLILEMGALSRLTGDPRFE AAL ALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMEDETTETSTSGCGSLILEMGALSRLTGDPRFEYAALHALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWS+RSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSLRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLV QNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVGQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHE+LPEVYGLTNGTSIKGENSSRR SAMSLQVCPATSLNTAGDG QQ+ESACHILDAR
Sbjct: 481 SWHEQLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGSQQIESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWL 574
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCG WL
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWL 573

BLAST of Carg10371 vs. NCBI nr
Match: XP_022135212.1 (alpha-mannosidase I MNS5 isoform X1 [Momordica charantia])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 547/575 (95.13%), Postives = 562/575 (97.74%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S+NYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPCRSATWILLLFFIFSALFGPSTSDNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL RQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSL+LYT
Sbjct: 361 KYGVLPERYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPA+SLNTAGDG Q +ESACHILDAR
Sbjct: 481 SWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPASSLNTAGDGGQPIESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 ADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 575

BLAST of Carg10371 vs. ExPASy Swiss-Prot
Match: Q9SXC9 (Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 4.4e-263
Identity = 440/570 (77.19%), Postives = 498/570 (87.37%), Query Frame = 0

Query: 3   PRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDEL 62
           PRR F  +L+    F  + +PSS   +   K+ +MREKVR MFYHAYDNYMTYAFPHDEL
Sbjct: 7   PRRLFLCLLISLTFF--VVDPSS--QHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDEL 66

Query: 63  KPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENL 122
           KPLTK+FT+SLSELGNLKLEHLP +YNGSA+TL+ESLSSL ILGN+TEF+K VLWLSENL
Sbjct: 67  KPLTKSFTDSLSELGNLKLEHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENL 126

Query: 123 RFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPAFN 182
            FD+DAR+NLFECNIRVLGGL+SAH+LA D  NRL + SY NQLL LAEDLG RFLPAF 
Sbjct: 127 TFDIDARVNLFECNIRVLGGLISAHLLAIDPNNRLIQGSYNNQLLRLAEDLGKRFLPAFE 186

Query: 183 TPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRKLW 242
           TPTGLPYAWINLK+GVMENETTETS+SGCGSL+LEMGALSRLTGDPRFESAALRALR+LW
Sbjct: 187 TPTGLPYAWINLKNGVMENETTETSTSGCGSLVLEMGALSRLTGDPRFESAALRALRQLW 246

Query: 243 SMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAYLA 302
            MRSSL+LLGTTLDV TGEWIE+SS IGAGVDSFYEYL KA++LFGKE++WRMFHSAYLA
Sbjct: 247 RMRSSLDLLGTTLDVVTGEWIEYSSSIGAGVDSFYEYLLKAYILFGKEDYWRMFHSAYLA 306

Query: 303 VQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKY 362
            QKYFRHGPWYHEA+M +G+ T+WQLTSLQAFWPGLQVLVGDI AANSSHREFFHVWEK+
Sbjct: 307 SQKYFRHGPWYHEANMWSGKPTYWQLTSLQAFWPGLQVLVGDIAAANSSHREFFHVWEKF 366

Query: 363 GVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKV 422
           GVLPERYLLD+Q++HPT KYYPLRPELAESTFYLYQATKDPWY +VGES++KSL LYTKV
Sbjct: 367 GVLPERYLLDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKV 426

Query: 423 EGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLSSW 482
            GGFASVRDVTTMQLEDHQHSFFL+ETCKYLYLLFDDSF+  +NYIFTTEGHP+ V+SSW
Sbjct: 427 PGGFASVRDVTTMQLEDHQHSFFLAETCKYLYLLFDDSFVAKRNYIFTTEGHPIQVVSSW 486

Query: 483 HERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDARAD 542
           HE+LPE Y   N T  K      R+SA+SLQVCP  SLN+    +QQ ESACH+LD + +
Sbjct: 487 HEKLPETYFSGNWTLSKSGAWESRASALSLQVCPLISLNSR-HPEQQRESACHVLDEQIN 546

Query: 543 HKCFSDEECGVDSTTCRRRSCSSSGYCGHW 573
           HKC+S++ECGVD+TTCR R+CS  GYCG W
Sbjct: 547 HKCWSNKECGVDATTCRLRTCSGVGYCGLW 571

BLAST of Carg10371 vs. ExPASy Swiss-Prot
Match: Q9FG93 (Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.9e-113
Identity = 219/485 (45.15%), Postives = 306/485 (63.09%), Query Frame = 0

Query: 6   SFTWIL---LLFIIFS--ALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 65
           +F W+L   L+ + FS   L +   + +    +  ++R++VR MFYHA+D YM  AFP D
Sbjct: 4   NFKWLLFAILISLTFSGFVLHHGVLAESVKPDEAKQLRDEVRGMFYHAFDGYMNNAFPLD 63

Query: 66  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 125
           EL+PL+    ++L                G ALTLI+SL +L +LG+   F  +V W+ +
Sbjct: 64  ELRPLSCQGEDTL---------------GGYALTLIDSLDTLALLGDRERFTSSVEWIGK 123

Query: 126 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 185
           NL+F+++  +++FE  IRVLGGL+SAH++A+D    +   SY N+LLVLAE+L  R LPA
Sbjct: 124 NLQFNINKTVSVFETTIRVLGGLLSAHLIASDYATGMRIPSYNNELLVLAENLARRMLPA 183

Query: 186 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 245
           F+TPTG+P+  +NL +GV ++E+  TS++G G+L LE G LSRLT DP FE  A  A+R 
Sbjct: 184 FDTPTGIPFGSVNLMYGVDKHESKITSTAGGGTLSLEFGVLSRLTNDPVFEQVAKNAVRG 243

Query: 246 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 305
           LW+ RS+L+L+G  ++V TGEW +  +GIG  +DSFYEYL KA++LFG EE+  +F  AY
Sbjct: 244 LWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYILFGDEEYLYIFQEAY 303

Query: 306 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 365
            +  +Y    PWY E +M +         SLQAFWPGLQVL GD+  A  +H  FF VW+
Sbjct: 304 RSAMQYLHKDPWYVEVNMDSAAIVWPVFNSLQAFWPGLQVLAGDVDPAIRTHTAFFSVWK 363

Query: 366 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 425
           +YG  PE + L    +   +K YPLRPEL EST++LY+AT+DP Y + G   + SL    
Sbjct: 364 RYGFTPEGFNLATLSVQYGQKSYPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGA 423

Query: 426 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLF----DDSFLVDQ---NYIFTTEG 479
           K   G+  + DV   + EDH  SFFL+ET KYL+LLF    D   LVD     YIF+TEG
Sbjct: 424 KCPCGYCHITDVELHKQEDHMESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEG 473

BLAST of Carg10371 vs. ExPASy Swiss-Prot
Match: Q92611 (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=9606 GN=EDEM1 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 6.1e-111
Identity = 224/506 (44.27%), Postives = 316/506 (62.45%), Query Frame = 0

Query: 34  RMRMREKVRRMFYHAYDNYMTYAFPHDELKPL-TKTFTNSLSELGNLKLEHLPQNYNGSA 93
           R +MR+  R MF   YDNYM +AFP DEL P+  +       +  NL +  +  NY   +
Sbjct: 127 RAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNY---S 186

Query: 94  LTLIESLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATD 153
           LTL+++L +L I+GN++EFQKAV  +   + FD D+ + +FE  IRVLG L+SAH + TD
Sbjct: 187 LTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITD 246

Query: 154 ST---NRLPRQSYKNQLLVLAEDLGNRFLPAF-NTPTGLPYAWINLKHGVMENETTETSS 213
           S      +  + Y N+LL +A DL  R LPAF NT TG+PY  +NLK GV  +   ET +
Sbjct: 247 SKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCT 306

Query: 214 SGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSS-LNLLGTTLDVETGEWIEFSS 273
           +G GSL++E G LSRL GD  FE  A RA++ LW++RS+   LLG  ++++TG W+   S
Sbjct: 307 AGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQS 366

Query: 274 GIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAYLAVQKYFRHG-----------PWYHEA 333
           G+GAG+DSFYEYL K+++LFG++E   MF++AY ++Q Y R G           P Y   
Sbjct: 367 GLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNV 426

Query: 334 DMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKYGVLPERYLLDYQML 393
           +M +G+  +  + SLQAF+PGLQVL+GD+  A   H  ++ +W++YG LPERY  ++Q+ 
Sbjct: 427 NMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERY--NWQLQ 486

Query: 394 HPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDVTTMQ 453
            P   +YPLRPEL EST+ LYQATK+P+Y  VG  I++SL  YTKV+ G+A++  V    
Sbjct: 487 APDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKS 546

Query: 454 LEDHQHSFFLSETCKYLYLLFDDSFLVDQN---YIFTTEGHPLPVLSSWHERLPEVYGLT 513
            ED   SFFLSETCKYLYLLFD+   V ++   Y+FTTEGH + V     E   + +   
Sbjct: 547 TEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSE 606

Query: 514 NGTSIKGENSSRRSSAMSLQVCPATS 520
            G   +G  S  R     L+V  ++S
Sbjct: 607 EGGQDQGGKSVHRPKPHELKVINSSS 627

BLAST of Carg10371 vs. ExPASy Swiss-Prot
Match: Q925U4 (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 6.1e-111
Identity = 223/506 (44.07%), Postives = 317/506 (62.65%), Query Frame = 0

Query: 34  RMRMREKVRRMFYHAYDNYMTYAFPHDELKPL-TKTFTNSLSELGNLKLEHLPQNYNGSA 93
           R +MR+  R MF   YDNYM +AFP DEL P+  +       +  NL +  +  NY   +
Sbjct: 122 RAQMRDLARGMFVFGYDNYMAHAFPQDELNPIYCRGRGPDRGDPSNLNINDVLGNY---S 181

Query: 94  LTLIESLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATD 153
           LTL+++L +L I+GN++EFQKAV  +   + FD D+ + +FE  IRVLG L+SAH + TD
Sbjct: 182 LTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITD 241

Query: 154 ST---NRLPRQSYKNQLLVLAEDLGNRFLPAF-NTPTGLPYAWINLKHGVMENETTETSS 213
           S      +  + Y N+LL +A DL  R LPAF NT TG+PY  +NLK GV  +   ET +
Sbjct: 242 SKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDSNNETCT 301

Query: 214 SGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSS-LNLLGTTLDVETGEWIEFSS 273
           +G GSL++E G LSRL GD  FE  A RA++ LW++RS+   LLG  ++++TG W+   S
Sbjct: 302 AGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQS 361

Query: 274 GIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAYLAVQKYFRHG-----------PWYHEA 333
           G+GAG+DSFYEYL K+++LFG++E   MF++AY ++Q Y R G           P Y   
Sbjct: 362 GLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNV 421

Query: 334 DMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKYGVLPERYLLDYQML 393
           +M +G+  +  + SLQAF+PGLQVL+GD+  A   H  ++ +W++YG LPERY  ++Q+ 
Sbjct: 422 NMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERY--NWQLQ 481

Query: 394 HPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDVTTMQ 453
            P   +YPLRPEL EST+ LYQATK+P+Y  VG  I++SL  YTKV+ G+A++  V    
Sbjct: 482 APDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKS 541

Query: 454 LEDHQHSFFLSETCKYLYLLFDDSFLVDQN---YIFTTEGHPLPVLSSWHERLPEVYGLT 513
            ED   SFFLSETCKYLYLLFD+   V ++   Y+FTTEGH + V     E   + +   
Sbjct: 542 KEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTTEGHIISVDKRLRELPWKEFFSE 601

Query: 514 NGTSIKGENSSRRSSAMSLQVCPATS 520
           +G   + E    R  +  L+V  ++S
Sbjct: 602 DGERDQEEKFVHRPKSQELRVINSSS 622

BLAST of Carg10371 vs. ExPASy Swiss-Prot
Match: Q8BJT9 (ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=10090 GN=Edem2 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.5e-96
Identity = 200/514 (38.91%), Postives = 285/514 (55.45%), Query Frame = 0

Query: 38  REKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIE 97
           RE+V+ MFYHAYD+Y+  AFP+DEL+PLT    ++               +   +LTLI+
Sbjct: 36  RERVKAMFYHAYDSYLENAFPYDELRPLTCDGHDT---------------WGSFSLTLID 95

Query: 98  SLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRL 157
           +L +L+ILGN +EFQ+ V  L +N+ FD+D   ++FE NIRV+GGL+SAH+L+  +   +
Sbjct: 96  ALDTLLILGNTSEFQRVVEVLQDNVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEV 155

Query: 158 PR-QSYKNQLLVLAEDLGNRFLPAFNTPTGLPYAWINLKHGVMENETTETSSSGCGSLIL 217
                    LL +AE+   + LPAF TPTG+PY  +NL HGV   ET  T ++G G+ I+
Sbjct: 156 EAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIV 215

Query: 218 EMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSF 277
           E   LS LTGDP FE  A  AL +LW  RS + L+G  +DV TG+W+   +GIGAGVDS+
Sbjct: 216 EFATLSSLTGDPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSY 275

Query: 278 YEYLFKAHVLFGKEEFWRMFHSAYLAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWP 337
           +EYL K  +L   ++   MF     A++ Y     WY    M  G  +     SL+A+WP
Sbjct: 276 FEYLVKGAILLQDKKLMAMFLEYNKAIRNYTHFDDWYLWVQMYKGTVSMPVFQSLEAYWP 335

Query: 338 GLQVLVGDIPAANSSHREFFHVWEKYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYL 397
           GLQ L+GDI  A  +   ++ VW+++G LPE Y +         + YPLRPEL ES  YL
Sbjct: 336 GLQSLIGDIDNAMRTFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYL 395

Query: 398 YQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLL 457
           Y+AT DP   E+G   ++S+   +KVE GFA+++D+   +L++   SFFL+ET KYLYLL
Sbjct: 396 YRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLL 455

Query: 458 FDDSFLVDQN--------------------YIFTTEGHPLPVLS----------SW--HE 517
           F  +  +  N                    YIF TE HP+   +           W   +
Sbjct: 456 FHPNNFIHNNGSTFDSVMTPHGECILGAGGYIFNTEAHPIDPAALHCCRRLKEEQWEVED 515

Query: 518 RLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPAT 519
            + E Y L      + +  + RS     Q  PAT
Sbjct: 516 LIKEFYSLKQSRPKRAQRKTVRSGPWEPQSGPAT 534

BLAST of Carg10371 vs. ExPASy TrEMBL
Match: A0A6J1E1J2 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111429983 PE=3 SV=1)

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 574/575 (99.83%), Postives = 574/575 (99.83%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NLRFDVDARINLFECNIRVLGGLVSAHVLATDST RLPRQSYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTTRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of Carg10371 vs. ExPASy TrEMBL
Match: A0A6J1JLB4 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111485626 PE=3 SV=1)

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPR+SYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRESYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALS+LTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSQLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT
Sbjct: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFS+EECGVDSTTCRRRSCSSSGYCGHWLRL
Sbjct: 541 ADHKCFSNEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of Carg10371 vs. ExPASy TrEMBL
Match: A0A6J1C218 (alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111007230 PE=3 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 547/575 (95.13%), Postives = 562/575 (97.74%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S+NYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPCRSATWILLLFFIFSALFGPSTSDNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL RQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSL+LYT
Sbjct: 361 KYGVLPERYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPA+SLNTAGDG Q +ESACHILDAR
Sbjct: 481 SWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPASSLNTAGDGGQPIESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 ADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 575

BLAST of Carg10371 vs. ExPASy TrEMBL
Match: A0A1S3BWL9 (alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103493935 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 544/575 (94.61%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           ML RRSF WILLLFII   LFNPSSS++YWT+KRMRMREK RRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLSRRSFPWILLLFIILFTLFNPSSSSSYWTSKRMRMREKARRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNY GSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYTGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NL FDVDARINLFECNIRVLGGL+SAHVLATDSTNRL +QSYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLISAHVLATDSTNRLAKQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGV+E+ETTETS+SGCGSLILEMGALSRLTGDPRFE AALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVVEDETTETSTSGCGSLILEMGALSRLTGDPRFEYAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYL KAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLLKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLD QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLI +T
Sbjct: 361 KYGVLPERYLLDRQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLISHT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRR SAMSLQVCPATSLNTAGDG QQ+ESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGGQQIESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCG WL L
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWLHL 575

BLAST of Carg10371 vs. ExPASy TrEMBL
Match: A0A0A0LNZ0 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_1G005710 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 546/575 (94.96%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           ML RRSF WILLLFIIF  LFNPSSS++Y T+KRMRMREK RRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLSRRSFAWILLLFIIFFTLFNPSSSSSYLTSKRMRMREKARRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTNSLSELGNLKLEHLPQNY GSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYTGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL RQSYKNQLLVLAEDLGNRFLPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVME+ETTETS+SGCGSLILEMGALSRLTGDPRFE AALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMEDETTETSTSGCGSLILEMGALSRLTGDPRFEHAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYL KAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLLKAHVLFGKEEFWRMFHAAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 420
           KYGVLPERYLLD QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESI+KSLI +T
Sbjct: 361 KYGVLPERYLLDRQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIIKSLISHT 420

Query: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480
           KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS
Sbjct: 421 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLS 480

Query: 481 SWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDAR 540
           SWHERLPEVYGLTNGTSIKGENSSRR SAMSLQVCPATSLNTAGDG QQ+ESACHILDAR
Sbjct: 481 SWHERLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGGQQIESACHILDAR 540

Query: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 576
           ADHKCFSDEECGVDSTTCRRRSCSSSGYCG WL L
Sbjct: 541 ADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWLHL 575

BLAST of Carg10371 vs. TAIR 10
Match: AT1G27520.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 908.3 bits (2346), Expect = 3.2e-264
Identity = 440/570 (77.19%), Postives = 498/570 (87.37%), Query Frame = 0

Query: 3   PRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDEL 62
           PRR F  +L+    F  + +PSS   +   K+ +MREKVR MFYHAYDNYMTYAFPHDEL
Sbjct: 7   PRRLFLCLLISLTFF--VVDPSS--QHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDEL 66

Query: 63  KPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENL 122
           KPLTK+FT+SLSELGNLKLEHLP +YNGSA+TL+ESLSSL ILGN+TEF+K VLWLSENL
Sbjct: 67  KPLTKSFTDSLSELGNLKLEHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENL 126

Query: 123 RFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPAFN 182
            FD+DAR+NLFECNIRVLGGL+SAH+LA D  NRL + SY NQLL LAEDLG RFLPAF 
Sbjct: 127 TFDIDARVNLFECNIRVLGGLISAHLLAIDPNNRLIQGSYNNQLLRLAEDLGKRFLPAFE 186

Query: 183 TPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRKLW 242
           TPTGLPYAWINLK+GVMENETTETS+SGCGSL+LEMGALSRLTGDPRFESAALRALR+LW
Sbjct: 187 TPTGLPYAWINLKNGVMENETTETSTSGCGSLVLEMGALSRLTGDPRFESAALRALRQLW 246

Query: 243 SMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAYLA 302
            MRSSL+LLGTTLDV TGEWIE+SS IGAGVDSFYEYL KA++LFGKE++WRMFHSAYLA
Sbjct: 247 RMRSSLDLLGTTLDVVTGEWIEYSSSIGAGVDSFYEYLLKAYILFGKEDYWRMFHSAYLA 306

Query: 303 VQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKY 362
            QKYFRHGPWYHEA+M +G+ T+WQLTSLQAFWPGLQVLVGDI AANSSHREFFHVWEK+
Sbjct: 307 SQKYFRHGPWYHEANMWSGKPTYWQLTSLQAFWPGLQVLVGDIAAANSSHREFFHVWEKF 366

Query: 363 GVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKV 422
           GVLPERYLLD+Q++HPT KYYPLRPELAESTFYLYQATKDPWY +VGES++KSL LYTKV
Sbjct: 367 GVLPERYLLDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKV 426

Query: 423 EGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPLPVLSSW 482
            GGFASVRDVTTMQLEDHQHSFFL+ETCKYLYLLFDDSF+  +NYIFTTEGHP+ V+SSW
Sbjct: 427 PGGFASVRDVTTMQLEDHQHSFFLAETCKYLYLLFDDSFVAKRNYIFTTEGHPIQVVSSW 486

Query: 483 HERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGDQQLESACHILDARAD 542
           HE+LPE Y   N T  K      R+SA+SLQVCP  SLN+    +QQ ESACH+LD + +
Sbjct: 487 HEKLPETYFSGNWTLSKSGAWESRASALSLQVCPLISLNSR-HPEQQRESACHVLDEQIN 546

Query: 543 HKCFSDEECGVDSTTCRRRSCSSSGYCGHW 573
           HKC+S++ECGVD+TTCR R+CS  GYCG W
Sbjct: 547 HKCWSNKECGVDATTCRLRTCSGVGYCGLW 571

BLAST of Carg10371 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-114
Identity = 219/485 (45.15%), Postives = 306/485 (63.09%), Query Frame = 0

Query: 6   SFTWIL---LLFIIFS--ALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 65
           +F W+L   L+ + FS   L +   + +    +  ++R++VR MFYHA+D YM  AFP D
Sbjct: 4   NFKWLLFAILISLTFSGFVLHHGVLAESVKPDEAKQLRDEVRGMFYHAFDGYMNNAFPLD 63

Query: 66  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 125
           EL+PL+    ++L                G ALTLI+SL +L +LG+   F  +V W+ +
Sbjct: 64  ELRPLSCQGEDTL---------------GGYALTLIDSLDTLALLGDRERFTSSVEWIGK 123

Query: 126 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 185
           NL+F+++  +++FE  IRVLGGL+SAH++A+D    +   SY N+LLVLAE+L  R LPA
Sbjct: 124 NLQFNINKTVSVFETTIRVLGGLLSAHLIASDYATGMRIPSYNNELLVLAENLARRMLPA 183

Query: 186 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 245
           F+TPTG+P+  +NL +GV ++E+  TS++G G+L LE G LSRLT DP FE  A  A+R 
Sbjct: 184 FDTPTGIPFGSVNLMYGVDKHESKITSTAGGGTLSLEFGVLSRLTNDPVFEQVAKNAVRG 243

Query: 246 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 305
           LW+ RS+L+L+G  ++V TGEW +  +GIG  +DSFYEYL KA++LFG EE+  +F  AY
Sbjct: 244 LWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYILFGDEEYLYIFQEAY 303

Query: 306 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 365
            +  +Y    PWY E +M +         SLQAFWPGLQVL GD+  A  +H  FF VW+
Sbjct: 304 RSAMQYLHKDPWYVEVNMDSAAIVWPVFNSLQAFWPGLQVLAGDVDPAIRTHTAFFSVWK 363

Query: 366 KYGVLPERYLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYT 425
           +YG  PE + L    +   +K YPLRPEL EST++LY+AT+DP Y + G   + SL    
Sbjct: 364 RYGFTPEGFNLATLSVQYGQKSYPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGA 423

Query: 426 KVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLF----DDSFLVDQ---NYIFTTEG 479
           K   G+  + DV   + EDH  SFFL+ET KYL+LLF    D   LVD     YIF+TEG
Sbjct: 424 KCPCGYCHITDVELHKQEDHMESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEG 473

BLAST of Carg10371 vs. TAIR 10
Match: AT1G51590.1 (alpha-mannosidase 1 )

HSP 1 Score: 199.9 bits (507), Expect = 5.5e-51
Identity = 147/478 (30.75%), Postives = 233/478 (48.74%), Query Frame = 0

Query: 38  REKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIE 97
           R+KV+    HA+ +Y  YA+  DEL+P TK  T+S   LG                T+++
Sbjct: 98  RQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLG---------------ATMVD 157

Query: 98  SLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRL 157
           SL +L I+G + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA+ L+ D     
Sbjct: 158 SLDTLYIMGLDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGD----- 217

Query: 158 PRQSYKNQLLVLAEDLGNRFLPAFNTPTGLPYAWINLKHGVMENET----TETSSSGCGS 217
                    L  A+D+ +R LPA+NTPTG+PY  INL++G   N +     ++  +  G+
Sbjct: 218 ------KMFLEKAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGT 277

Query: 218 LILEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGV 277
             LE  ALS+ TGDP+++    + + +L     +  LL   ++ +       ++  GA  
Sbjct: 278 EQLEFIALSQRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMG 337

Query: 278 DSFYEYLFKAHVLFGK-------EEFWRMFHSAYLAVQKYFRHGPWYHEADMRTGRATHW 337
           DSFYEYL K  V   K        + W       L++ K      + +  + + G     
Sbjct: 338 DSFYEYLLKVWVQGNKTSAVKPYRDMWEKSMKGLLSLVKKSTPSSFTYICE-KNGNNLID 397

Query: 338 QLTSLQAFWPGLQVLVGDIPAANSSHREFFHV-----WEKYG--------VLPERYLLDY 397
           ++  L  F PG+  L G         ++F  +     W  Y         +  E Y    
Sbjct: 398 KMDELACFAPGMLAL-GASGYGPDEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTA 457

Query: 398 -QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDV 457
            Q +     +  LRPE  ES FYL++ T +  Y E G +I ++    ++VE G+  ++DV
Sbjct: 458 GQDMSVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDV 517

Query: 458 TTMQLEDHQHSFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLSSWHERLPEV 490
            T   ++   SFFL+ET KYLYLLF  S ++    ++F TE HPL +++    R P +
Sbjct: 518 NTGAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKIVARNDPRKPTI 547

BLAST of Carg10371 vs. TAIR 10
Match: AT1G51590.2 (alpha-mannosidase 1 )

HSP 1 Score: 194.5 bits (493), Expect = 2.3e-49
Identity = 144/469 (30.70%), Postives = 228/469 (48.61%), Query Frame = 0

Query: 47  HAYDNYMTYAFPHDELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILG 106
           HA+ +Y  YA+  DEL+P TK  T+S   LG                T+++SL +L I+G
Sbjct: 3   HAWSSYEKYAWGKDELQPRTKDGTDSFGGLG---------------ATMVDSLDTLYIMG 62

Query: 107 NNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQL 166
            + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA+ L+ D              
Sbjct: 63  LDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGD-----------KMF 122

Query: 167 LVLAEDLGNRFLPAFNTPTGLPYAWINLKHGVMENET----TETSSSGCGSLILEMGALS 226
           L  A+D+ +R LPA+NTPTG+PY  INL++G   N +     ++  +  G+  LE  ALS
Sbjct: 123 LEKAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 182

Query: 227 RLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFK 286
           + TGDP+++    + + +L     +  LL   ++ +       ++  GA  DSFYEYL K
Sbjct: 183 QRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLK 242

Query: 287 AHVLFGK-------EEFWRMFHSAYLAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFW 346
             V   K        + W       L++ K      + +  + + G     ++  L  F 
Sbjct: 243 VWVQGNKTSAVKPYRDMWEKSMKGLLSLVKKSTPSSFTYICE-KNGNNLIDKMDELACFA 302

Query: 347 PGLQVLVGDIPAANSSHREFFHV-----WEKYG--------VLPERYLLDY-QMLHPTEK 406
           PG+  L G         ++F  +     W  Y         +  E Y     Q +     
Sbjct: 303 PGMLAL-GASGYGPDEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAGQDMSVGTS 362

Query: 407 YYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQ 466
           +  LRPE  ES FYL++ T +  Y E G +I ++    ++VE G+  ++DV T   ++  
Sbjct: 363 WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGAKDNKM 422

Query: 467 HSFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLSSWHERLPEV 490
            SFFL+ET KYLYLLF  S ++    ++F TE HPL +++    R P +
Sbjct: 423 QSFFLAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKIVARNDPRKPTI 443

BLAST of Carg10371 vs. TAIR 10
Match: AT3G21160.1 (alpha-mannosidase 2 )

HSP 1 Score: 191.4 bits (485), Expect = 2.0e-48
Identity = 143/474 (30.17%), Postives = 229/474 (48.31%), Query Frame = 0

Query: 32  AKRMRMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTNSLSELGNLKLEHLPQNYNGS 91
           A+RM   ++V+    HA+ +Y  YA+  DEL+P TK   +S   LG              
Sbjct: 96  AQRM---QRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLG-------------- 155

Query: 92  ALTLIESLSSLVILGNNTEFQKAVLWLSENLRFDVDARINLFECNIRVLGGLVSAHVLAT 151
             T+I++L +L I+G + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA+ L+ 
Sbjct: 156 -ATMIDALDTLYIMGLDEQFQKAREWVASSLDFDKDYAASMFETTIRVVGGLLSAYDLSG 215

Query: 152 DSTNRLPRQSYKNQLLVLAEDLGNRFLPAFNTPTGLPYAWINLKHGVMENETTETSSS-- 211
           D              L  A D+ +R LPA++T +G+PY  INLKHG   N T     S  
Sbjct: 216 DKI-----------FLEKAMDIADRLLPAWDTQSGIPYNIINLKHGNAHNPTWAGGDSIL 275

Query: 212 -GCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSG 271
              G+  LE  ALS+ TGDP+++    + +  L     +  LL   ++ +T    + +  
Sbjct: 276 ADSGTEQLEFIALSQRTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTANPSQSTIT 335

Query: 272 IGAGVDSFYEYLFKAHVLFGK-------EEFWRMFHSAYLAVQKYFRHGPWYHEADMRTG 331
            GA  DSFYEYL K  V   K        + W    +  L++ K      + +  + ++G
Sbjct: 336 FGAMGDSFYEYLLKVWVFGNKTSAVKHYRDMWEKSMNGLLSLVKKSTPLSFTYICE-KSG 395

Query: 332 RATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWEKYGVLPERYLLDYQMLHPTEK 391
            +   ++  L  F PG+  L     +  +  ++F  + E+       +          E 
Sbjct: 396 NSLIDKMDELACFAPGMLALGASGYSDPAEGKKFLTLAEELAWTCYNFYQSTPTKLAGEN 455

Query: 392 YY--------------PLRPELAESTFYLYQATKDPWYFEVGESIMKSLILYTKVEGGFA 451
           Y+               LRPE  ES FYL++ T +  Y E G +I ++    +++E G+ 
Sbjct: 456 YFFNSGSDMSVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFEAFEKNSRIESGYV 515

Query: 452 SVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLS 481
            ++DV T   ++   SFFL+ET KYLYLLF  + ++    ++F TE HPL + S
Sbjct: 516 GLKDVNTGVKDNKMQSFFLAETLKYLYLLFSPTTVIPLDEWVFNTEAHPLKIKS 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023516302.10.0e+00100.00alpha-mannosidase I MNS5 [Cucurbita pepo subsp. pepo] >KAG7023152.1 Alpha-mannos... [more]
XP_022921842.10.0e+0099.83alpha-mannosidase I MNS5 [Cucurbita moschata] >KAG6589467.1 Alpha-mannosidase I ... [more]
XP_022988359.10.0e+0099.30alpha-mannosidase I MNS5 [Cucurbita maxima][more]
XP_038879418.10.0e+0096.51alpha-mannosidase I MNS5 [Benincasa hispida][more]
XP_022135212.10.0e+0095.13alpha-mannosidase I MNS5 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SXC94.4e-26377.19Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1[more]
Q9FG932.9e-11345.15Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1[more]
Q926116.1e-11144.27ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=960... [more]
Q925U46.1e-11144.07ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=100... [more]
Q8BJT92.5e-9638.91ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=100... [more]
Match NameE-valueIdentityDescription
A0A6J1E1J20.0e+0099.83alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111429983 PE=3 SV=1[more]
A0A6J1JLB40.0e+0099.30alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111485626 PE=3 SV=1[more]
A0A6J1C2180.0e+0095.13alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111007230 PE=3 SV=1[more]
A0A1S3BWL90.0e+0094.61alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103493935 PE=3 SV=1[more]
A0A0A0LNZ00.0e+0094.96alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_1G005710 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G27520.13.2e-26477.19Glycosyl hydrolase family 47 protein [more]
AT5G43710.12.1e-11445.15Glycosyl hydrolase family 47 protein [more]
AT1G51590.15.5e-5130.75alpha-mannosidase 1 [more]
AT1G51590.22.3e-4930.70alpha-mannosidase 1 [more]
AT3G21160.12.0e-4830.17alpha-mannosidase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase family 47PRINTSPR00747GLYHDRLASE47coord: 327..343
score: 35.01
coord: 270..287
score: 58.2
coord: 443..463
score: 61.22
coord: 179..198
score: 54.29
coord: 384..408
score: 48.19
coord: 44..64
score: 53.97
coord: 129..147
score: 52.13
IPR001382Glycoside hydrolase family 47PFAMPF01532Glyco_hydro_47coord: 44..477
e-value: 1.4E-111
score: 373.6
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 26..488
e-value: 1.3E-153
score: 513.9
NoneNo IPR availablePANTHERPTHR45679:SF3ALPHA-MANNOSIDASE I MNS5coord: 19..573
IPR044674ER degradation-enhancing alpha-mannosidase-like protein 1/2/3PANTHERPTHR45679ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2coord: 19..573
IPR036026Seven-hairpin glycosidasesSUPERFAMILY48225Seven-hairpin glycosidasescoord: 34..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10371-RACarg10371-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:1904380 endoplasmic reticulum mannose trimming
biological_process GO:1904382 mannose trimming involved in glycoprotein ERAD pathway
cellular_component GO:0044322 endoplasmic reticulum quality control compartment
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity