Carg10333 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10333
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Kinetochore protein SPC25
Location	Carg_Chr10: 459557 .. 461275 (-)
RNA-Seq Expression	Carg10333
Synteny	Carg10333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCGCTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGTGAGCCATCTATTCTCTTCAAATTATTCGGGAAAGAAGTGGATGCGATACCGTTATATTGTGGCGAGTTCAATAATGTAATCGGTAATAGAATTGTTTGTGAAGTCACCGATAGGGTTTTGAGATTGGTAGCGTACAAGACTCAAAAATGGATGTAATTCTAGGGGACTGAGTAGGTTTAGGTTCAGGAGTGGCCAAGTTGATTGATTTTTGAATTTTCAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGGTATGAGCTATGATTATGCTAATTTCCTCTAACGCTGCATGAGTAGAATACACTATTTCCTTGAATCGACTGAGGCTGCATGCTAATTATAAGGCTTATTTCCATTATAATTGCGTGTTTTTGTAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGGTAAACTTAATTTATTTACGAGTGCTCACTCTCAAATCTGTTCTTGCTAGCCCTTAAAAACTGATTACTTTTCCTCCTTTCCAATGCCATCAAAAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGTATGATAATTTGAGTTAGTTGTTTTGTAATAACCTTTATATTTTTTGTGAATTCTTGAGATTAATTTCTCGACAACTTGTCTCGGGTTTTCAGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTGTAAGTTATGTGCACTTAATGTATGTTCATTTTTCATGTTCTTGATCTTCTGACAACCTTACTTTTTCAGTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGTGTGCGATGAATTTTGAACTTTTTGCTTGAAAGTGTGTTATTCACTGCACAAAAAACTCATTGGTACTTAACAGACATACTACCTGAAGCACCATGTTTTGTGCTTCTTGCAGTTTGTTAGATTGAACTTGTTCTGCATTAATAGTTTGTTATATTTTTGTTATTCAACAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAG

mRNA sequence

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCGCTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAG

Coding sequence (CDS)

ATGGAGAACAAGGCTGAAGAATCTGTTTGTGCGAGGATGGAGTCGCTTCGAATTGTTCGTGAAAAGGAAATTGCAGTTCAGCAACAAAAAATGGAGTCCTTCGCGGATTCATTTCGCAAGTCTCTGGAATCGATCGCTGCACGGTCCCGAGAAAATGCCCGAAGTCAAGGAAAACTGGGGGAACTAAAAGCGAGGTTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTCAAGACTCGTAAACAGGCTAAACGCCTCGCACTCACGGACTCCCTTGCAACTTCAAAATCTAGAATTGAAGAGCTCAGAAACAATTTACAAGACTGCAAAGCAAGGAGGGATGAATATTCAACAACTATATCTCGGCAATCCTTAGCTTTATCTCCCTATGAACACAAGGAACCCCAAGAGAGAGATTGCAGAAATGAAATTCAAGAGGCTATTTCTTGGTACAATAGAGTTCTTGATTTCCATATTGAAGGAGGGCATGGGGTAAAGTTCTCTTTCAAGAAAATCAATGTAGACGATCCTGATAAAGAATATTCTTTTACCATCCGTCATGCAAATGATACTTATACGTTGTTAGATTGTAACCCATCCTTGCAAGGTATTGAAGAGTTGATACATGAATTAAATAATACAAATGGGTTATTCAAGTTTGTCAGGATCATGAGACGGAGGTTCCAGGAGGCTGCTGCAGAAGGAGTTGGGGCCCAAGCATTGTCTCTGCATCAAGGCTCGACGATAATCTCCATGTCAGCTCCAGGTTTGACATCATCATTGGACAGAAGTGAATCTTCAACTGAAGAAATTGATAGCCAGGTTCAACTCGAGGAAACTAACAAACCTTCTAAGAAAACTTCATCCGGAAAGAAACCAGCTATCCAATCCCTTGGATCTGCATTTTCTCTGCGTCGAACTCCTCGTTTCAAG

Protein sequence

MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLGELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPRFK

Homology

[Close]

BLAST of Carg10333 vs. NCBI nr
Match: KAG7023114.1 (spc25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.7 bits (1506), Expect = 4.7e-163
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Carg10333 vs. NCBI nr
Match: KAG6589434.1 (Kinetochore protein SPC25-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Carg10333 vs. NCBI nr
Match: XP_022921533.1 (probable kinetochore protein SPC25 isoform X2 [Cucurbita moschata])

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-159
Identity = 305/313 (97.44%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS+
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQSI 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Carg10333 vs. NCBI nr
Match: XP_023516085.1 (probable kinetochore protein SPC25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.1e-159
Identity = 305/313 (97.44%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRH NDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHGNDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEET+KPSKKTSSGKKPAIQSL
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETDKPSKKTSSGKKPAIQSL 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Carg10333 vs. NCBI nr
Match: XP_022921532.1 (probable kinetochore protein SPC25 isoform X1 [Cucurbita moschata])

HSP 1 Score: 568.5 bits (1464), Expect = 3.5e-158
Identity = 305/314 (97.13%), Postives = 309/314 (98.41%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TISRQSL ALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDP
Sbjct: 121 TISRQSLEALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFK 314
           +GSAFSLRRTPRFK
Sbjct: 301 IGSAFSLRRTPRFK 314

[Close]

BLAST of Carg10333 vs. ExPASy Swiss-Prot
Match: Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 129/302 (42.72%), Postives = 194/302 (64.24%), Query Frame = 0


Query: 13  MESLRIVREKEIAVQQQKMESF-ADSFRKSLESIAARSRENARSQGKLGELKARLREAED 72
           M SL ++ EK+I  Q+ K++SF A  FR+S+ S+  R++  A+SQ +L  LKA LREAED
Sbjct: 17  MASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKADLREAED 76

Query: 73  ELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSP 132
           ELVK LAVKTRK+A+++ + DS++ ++SRIE LR NLQ  K+++D+    IS+Q  ALS 
Sbjct: 77  ELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQLQALSK 136

Query: 133 YEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHAN 192
            +    +  + + +I EAISWYN  L FH+E GHGVKF+F  I+   P +E+SFT+ + N
Sbjct: 137 SKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSFTVHYGN 196

Query: 193 DTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTI 252
           D YTLLD +  L  I E++ ELN TN LF+FVR+MR +F ++    +   +  L Q ++ 
Sbjct: 197 DIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQLQQETSA 256

Query: 253 ISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPR 312
           IS SAP ++ S D + S+ E   S+VQ+      +++   G +  + +  S  + RR+ R
Sbjct: 257 ISASAPAISFSTDTNMSTPENKRSKVQV------NRRQKRGSESPLLAPVSTSATRRSSR 312

Query: 313 FK 314
           FK
Sbjct: 317 FK 312

[Close]

BLAST of Carg10333 vs. ExPASy Swiss-Prot
Match: Q10430 (Kinetochore protein spc25 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spc25 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 8.4e-06
Identity = 49/215 (22.79%), Postives = 103/215 (47.91%), Query Frame = 0


Query: 28  QQKMESFADSFRKSLESIAARSRENARSQ-GKLGELKARLREAEDELVKALAVK------ 87
           + K+ +F   F + L+    +   N      +L E+    ++AE  L +  A K      
Sbjct: 17  KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLSEINEAQKKAEKSLEQTEARKQNFTEL 76

Query: 88  TRKQAKRLALTD-SLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSPYEHKEPQE 147
             K+ +  A+T+  + + + +++ +    Q      D Y   IS +   L   E +  ++
Sbjct: 77  LEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEELDHYRAIISSKR-ELRAQEMEAKRK 136

Query: 148 RDCRNEIQEAISWYNRVLDFHIEGGHG--VKFSFKKINVDDPDKEYSFTIRHANDTYTLL 207
           +D  N  +  + ++   L   +EG H   ++F F  I+  D +K++SF I  A   Y ++
Sbjct: 137 QDSYNNPE--LKFWEDYLGLKMEGVHDEVIRFIFTNIDEKDWNKQFSFQINLAERDYKVV 196

Query: 208 DCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQE 233
            C+P L  +++L++++N T   ++F++ MR+ F+E
Sbjct: 197 HCHPPLPHVDDLVNKVNRTRDFYQFLKDMRKGFRE 228

[Close]

BLAST of Carg10333 vs. ExPASy TrEMBL
Match: A0A6J1E461 (Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.8e-160
Identity = 305/313 (97.44%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
           TISRQSLALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDPD
Sbjct: 121 TISRQSLALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA
Sbjct: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS+
Sbjct: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQSI 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRRTPRFK
Sbjct: 301 GSAFSLRRTPRFK 313

[Close]

BLAST of Carg10333 vs. ExPASy TrEMBL
Match: A0A6J1E0R4 (Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.7e-158
Identity = 305/314 (97.13%), Postives = 309/314 (98.41%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENKAEESVCARMESLRIVREKEIAV+QQKMESFADSFRKSLESI ARSRENARSQGKLG
Sbjct: 1   MENKAEESVCARMESLRIVREKEIAVEQQKMESFADSFRKSLESITARSRENARSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLALTDSLATSKSRIEELRNN QDCKARRDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLALTDSLATSKSRIEELRNNFQDCKARRDEYST 120

Query: 121 TISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TISRQSL ALSPYEHKEPQE DCRNEIQEAISWYNRVLDFHIEGGHGV FSFKKINVDDP
Sbjct: 121 TISRQSLEALSPYEHKEPQESDCRNEIQEAISWYNRVLDFHIEGGHGVNFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFK 314
           +GSAFSLRRTPRFK
Sbjct: 301 IGSAFSLRRTPRFK 314

[Close]

BLAST of Carg10333 vs. ExPASy TrEMBL
Match: A0A6J1JCY1 (Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 300/314 (95.54%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENAR-SQGKL 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKM+SFADSFRKSLESI ARSRENAR  QGKL
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMQSFADSFRKSLESITARSRENARIIQGKL 60

Query: 61  GELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120
           GELKARLR+AEDEL KALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS
Sbjct: 61  GELKARLRKAEDELAKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120

Query: 121 TTISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180
           TTISRQSLALSPYEHK+PQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP
Sbjct: 121 TTISRQSLALSPYEHKQPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDP 180

Query: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVG 240
           DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRI+RRRFQEAAAEGVG
Sbjct: 181 DKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIIRRRFQEAAAEGVG 240

Query: 241 AQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQS 300
           AQALSLHQGSTIISMSAPGLTSS DRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQS
Sbjct: 241 AQALSLHQGSTIISMSAPGLTSSSDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQS 300

Query: 301 LGSAFSLRRTPRFK 314
           LGSAFSLRRTPRFK
Sbjct: 301 LGSAFSLRRTPRFK 314

[Close]

BLAST of Carg10333 vs. ExPASy TrEMBL
Match: A0A6J1JH38 (Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 300/315 (95.24%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENAR-SQGKL 60
           MENKAE+SVC RMESLRIVREKEIAVQQQKM+SFADSFRKSLESI ARSRENAR  QGKL
Sbjct: 1   MENKAEDSVCERMESLRIVREKEIAVQQQKMQSFADSFRKSLESITARSRENARIIQGKL 60

Query: 61  GELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120
           GELKARLR+AEDEL KALAVKTRK+AKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS
Sbjct: 61  GELKARLRKAEDELAKALAVKTRKEAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYS 120

Query: 121 TTISRQSL-ALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD 180
           TTISRQSL ALSPYEHK+PQE DCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD
Sbjct: 121 TTISRQSLEALSPYEHKQPQESDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDD 180

Query: 181 PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGV 240
           PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRI+RRRFQEAAAEGV
Sbjct: 181 PDKEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIIRRRFQEAAAEGV 240

Query: 241 GAQALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQ 300
           GAQALSLHQGSTIISMSAPGLTSS DRSESSTEEID+QVQLEETNKPSKKTSSGKKPAIQ
Sbjct: 241 GAQALSLHQGSTIISMSAPGLTSSSDRSESSTEEIDNQVQLEETNKPSKKTSSGKKPAIQ 300

Query: 301 SLGSAFSLRRTPRFK 314
           SLGSAFSLRRTPRFK
Sbjct: 301 SLGSAFSLRRTPRFK 315

[Close]

BLAST of Carg10333 vs. ExPASy TrEMBL
Match: A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.6e-135
Identity = 264/313 (84.35%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MENKAEESVCARMESLRIVREKEIAVQQQKMESFADSFRKSLESIAARSRENARSQGKLG 60
           MENK E+S+  +MESLR+VREKEI VQQQKMESFADSFRKSLESI  RSRENA SQGKLG
Sbjct: 1   MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYST 120
           ELKARLREAEDELVKALAVKTRK+AKRLA+TDSLA SKSRIEELRN LQDCKA+RDEYST
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120

Query: 121 TISRQSLALSPYEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPD 180
            IS+QSLALS  EHKE QE DCR+EIQEAISWYNRVLDFHIEGG GVKFSFKKIN++ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180

Query: 181 KEYSFTIRHANDTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGA 240
           KEYSFTIRHA+DTYTLLDCNPSLQ I++LIHELN TNGLFKFVR+MR+RFQE AAEGVGA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240

Query: 241 QALSLHQGSTIISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSL 300
           QALSLHQ STIIS+SAPGL+SS DRSESST+EID+QV+LEETNK SKK  SGKKPA+ S 
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300

Query: 301 GSAFSLRRTPRFK 314
           GSAFSLRR+PRFK
Sbjct: 301 GSAFSLRRSPRFK 313

[Close]

BLAST of Carg10333 vs. TAIR 10
Match: AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 211.8 bits (538), Expect = 7.7e-55
Identity = 129/302 (42.72%), Postives = 194/302 (64.24%), Query Frame = 0


Query: 13  MESLRIVREKEIAVQQQKMESF-ADSFRKSLESIAARSRENARSQGKLGELKARLREAED 72
           M SL ++ EK+I  Q+ K++SF A  FR+S+ S+  R++  A+SQ +L  LKA LREAED
Sbjct: 17  MASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKADLREAED 76

Query: 73  ELVKALAVKTRKQAKRLALTDSLATSKSRIEELRNNLQDCKARRDEYSTTISRQSLALSP 132
           ELVK LAVKTRK+A+++ + DS++ ++SRIE LR NLQ  K+++D+    IS+Q  ALS 
Sbjct: 77  ELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQLQALSK 136

Query: 133 YEHKEPQERDCRNEIQEAISWYNRVLDFHIEGGHGVKFSFKKINVDDPDKEYSFTIRHAN 192
            +    +  + + +I EAISWYN  L FH+E GHGVKF+F  I+   P +E+SFT+ + N
Sbjct: 137 SKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSFTVHYGN 196

Query: 193 DTYTLLDCNPSLQGIEELIHELNNTNGLFKFVRIMRRRFQEAAAEGVGAQALSLHQGSTI 252
           D YTLLD +  L  I E++ ELN TN LF+FVR+MR +F ++    +   +  L Q ++ 
Sbjct: 197 DIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQLQQETSA 256

Query: 253 ISMSAPGLTSSLDRSESSTEEIDSQVQLEETNKPSKKTSSGKKPAIQSLGSAFSLRRTPR 312
           IS SAP ++ S D + S+ E   S+VQ+      +++   G +  + +  S  + RR+ R
Sbjct: 257 ISASAPAISFSTDTNMSTPENKRSKVQV------NRRQKRGSESPLLAPVSTSATRRSSR 312

Query: 313 FK 314
           FK
Sbjct: 317 FK 312

The following BLAST results are available for this feature:

BLAST of Carg10333 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023114.1	4.7e-163	100.00	spc25, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6589434.1	1.4e-162	99.68	Kinetochore protein SPC25-like protein, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022921533.1	1.4e-159	97.44	probable kinetochore protein SPC25 isoform X2 [Cucurbita moschata]	[more]
XP_023516085.1	3.1e-159	97.44	probable kinetochore protein SPC25 [Cucurbita pepo subsp. pepo]	[more]
XP_022921532.1	3.5e-158	97.13	probable kinetochore protein SPC25 isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg10333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK9	1.1e-53	42.72	Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ...	[more]
Q10430	8.4e-06	22.79	Kinetochore protein spc25 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)...	[more]

BLAST of Carg10333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E461	6.8e-160	97.44	Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=...	[more]
A0A6J1E0R4	1.7e-158	97.13	Kinetochore protein SPC25 OS=Cucurbita moschata OX=3662 GN=LOC111429767 PE=3 SV=...	[more]
A0A6J1JCY1	1.0e-155	95.54	Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1	[more]
A0A6J1JH38	2.5e-154	95.24	Kinetochore protein SPC25 OS=Cucurbita maxima OX=3661 GN=LOC111485654 PE=3 SV=1	[more]
A0A6J1BYG5	2.6e-135	84.35	Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV...	[more]

BLAST of Carg10333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48210.1	7.7e-55	42.72	CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 94..121
None	No IPR available	COILS	Coil	Coil	coord: 52..86
None	No IPR available	GENE3D	3.30.457.50	Chromosome segregation protein Spc25	coord: 139..236 e-value: 1.2E-26 score: 94.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..305
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..313
None	No IPR available	PANTHER	PTHR14281:SF3	SUBFAMILY NOT NAMED	coord: 1..313
IPR013255	Chromosome segregation protein Spc25, C-terminal	PFAM	PF08234	Spindle_Spc25	coord: 163..231 e-value: 5.3E-23 score: 81.0
IPR045143	Kinetochore protein Spc25	PANTHER	PTHR14281	KINETOCHORE PROTEIN SPC25-RELATED	coord: 1..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10333-RA	Carg10333-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0051301	cell division
biological_process	GO:0007059	chromosome segregation
cellular_component	GO:0031262	Ndc80 complex
cellular_component	GO:0005634	nucleus