Carg10227 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10227
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Peroxisomal membrane protein PEX16
Location	Carg_Chr17: 30791 .. 31351 (-)
RNA-Seq Expression	Carg10227
Synteny	Carg10227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGACTTGGTTCAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGTGCATACATGCTTCAAGATTTAAACTTTTTGAGTCGAGAATTTCTCGACTTCAAACTTATAAGTCTTGGATTGAAACCGATTATGCTGAATGTTTTTTCAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGGTTCGAAGTGTATCCATTTGCGATAGTTGTAGATTTAAGATGATGTGGATTATAGCTGATATTTACTGTGTTGATGTGCAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGAGATGAGAGGGAAATTGGAGTTTGAAGGGCCCAAGTCTTCCCTCCTGGTTTGTTGTTTCTACGCTTCATACAAATATTTGGTGACGTTTCTTTTTGCAATCAGCTATTCTGAATAATAATAATAAGAATAATAATAATAATAATAATAATAATAATAATAATAATAATA

mRNA sequence

ATGTTTGACTTGGTTCAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGAGATGAGAGGGAAATTGGAGTTTGAAGGGCCCAAGTCTTCCCTCCTGGTTTGTTGTTTCTACGCTTCATACAAATATTTGGTGACGTTTCTTTTTGCAATCAGCTATTCTGAATAATAATAATAAGAATAATAATAATAATAATAATAATAATAATAATAATAATAATA

Coding sequence (CDS)

ATGTTTGACTTGGTTCAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGA

Protein sequence

MFDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTYMSAS

Homology

[Close]

BLAST of Carg10227 vs. NCBI nr
Match: KAG7013349.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 138.7 bits (348), Expect = 2.0e-29
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 1  MFDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 60
          MFDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ
Sbjct: 1  MFDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 60

Query: 61 TRYTYMSAS 70
          TRYTYMSAS
Sbjct: 61 TRYTYMSAS 69

[Close]

BLAST of Carg10227 vs. NCBI nr
Match: KAG7026034.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 129.8 bits (325), Expect = 9.1e-27
Identity = 62/69 (89.86%), Postives = 68/69 (98.55%), Query Frame = 0


Query: 1   MFDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 60
           +FDLVQLRRRKM WAFY+MRDPFFER+TRRKLEGAEKVL+PVPFVGFLTAKIVEL++GAQ
Sbjct: 339 VFDLVQLRRRKMQWAFYIMRDPFFERFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQ 398

Query: 61  TRYTYMSAS 70
           TRYTYMSAS
Sbjct: 399 TRYTYMSAS 407

[Close]

BLAST of Carg10227 vs. NCBI nr
Match: XP_022999826.1 (peroxisome biogenesis protein 16 isoform X1 [Cucurbita maxima])

HSP 1 Score: 129.4 bits (324), Expect = 1.2e-26
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 2   FDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQT 61
           FDLVQLRRRKMLWAFY+MRDPFF R+TRRKLEGAEKVL+PVPFVGFLTAKIVEL++GAQT
Sbjct: 326 FDLVQLRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQT 385

Query: 62  RYTYMSAS 70
           RYTYMSAS
Sbjct: 386 RYTYMSAS 393

[Close]

BLAST of Carg10227 vs. NCBI nr
Match: XP_022999828.1 (peroxisome biogenesis protein 16 isoform X3 [Cucurbita maxima])

HSP 1 Score: 129.4 bits (324), Expect = 1.2e-26
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 2   FDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQT 61
           FDLVQLRRRKMLWAFY+MRDPFF R+TRRKLEGAEKVL+PVPFVGFLTAKIVEL++GAQT
Sbjct: 262 FDLVQLRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQT 321

Query: 62  RYTYMSAS 70
           RYTYMSAS
Sbjct: 322 RYTYMSAS 329

[Close]

BLAST of Carg10227 vs. NCBI nr
Match: XP_022959429.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 128.3 bits (321), Expect = 2.6e-26
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY
Sbjct: 306 ELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 365

Query: 66  MSAS 70
           MSAS
Sbjct: 366 MSAS 369

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BLAST of Carg10227 vs. ExPASy Swiss-Prot
Match: Q8S8S1 (Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 3.2e-19
Identity = 43/64 (67.19%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRK++WA YLMRDPFF +YTR+KLE ++K LE +P +GFLT KIVEL+ GAQ+RYTY
Sbjct: 304 ELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVELLEGAQSRYTY 363

Query: 66  MSAS 70
           +S S
Sbjct: 364 ISGS 367

[Close]

BLAST of Carg10227 vs. ExPASy TrEMBL
Match: A0A6J1KE71 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 5.7e-27
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 2   FDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQT 61
           FDLVQLRRRKMLWAFY+MRDPFF R+TRRKLEGAEKVL+PVPFVGFLTAKIVEL++GAQT
Sbjct: 326 FDLVQLRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQT 385

Query: 62  RYTYMSAS 70
           RYTYMSAS
Sbjct: 386 RYTYMSAS 393

[Close]

BLAST of Carg10227 vs. ExPASy TrEMBL
Match: A0A6J1KKT7 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 5.7e-27
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 2   FDLVQLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQT 61
           FDLVQLRRRKMLWAFY+MRDPFF R+TRRKLEGAEKVL+PVPFVGFLTAKIVEL++GAQT
Sbjct: 262 FDLVQLRRRKMLWAFYIMRDPFFGRFTRRKLEGAEKVLQPVPFVGFLTAKIVELMVGAQT 321

Query: 62  RYTYMSAS 70
           RYTYMSAS
Sbjct: 322 RYTYMSAS 329

[Close]

BLAST of Carg10227 vs. ExPASy TrEMBL
Match: A0A6J1H4U6 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-26
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY
Sbjct: 242 ELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 301

Query: 66  MSAS 70
           MSAS
Sbjct: 302 MSAS 305

[Close]

BLAST of Carg10227 vs. ExPASy TrEMBL
Match: A0A6J1H817 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-26
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY
Sbjct: 306 ELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 365

Query: 66  MSAS 70
           MSAS
Sbjct: 366 MSAS 369

[Close]

BLAST of Carg10227 vs. ExPASy TrEMBL
Match: A0A5D3DLT0 (Peroxisomal membrane protein PEX16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00240 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-26
Identity = 62/64 (96.88%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELI+GAQTRYTY
Sbjct: 290 ELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIVGAQTRYTY 349

Query: 66  MSAS 70
           MSAS
Sbjct: 350 MSAS 353

[Close]

BLAST of Carg10227 vs. TAIR 10
Match: AT2G45690.1 (shrunken seed protein (SSE1) )

HSP 1 Score: 95.1 bits (235), Expect = 2.3e-20
Identity = 43/64 (67.19%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 6   QLRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTY 65
           +LRRRK++WA YLMRDPFF +YTR+KLE ++K LE +P +GFLT KIVEL+ GAQ+RYTY
Sbjct: 304 ELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVELLEGAQSRYTY 363

Query: 66  MSAS 70
           +S S
Sbjct: 364 ISGS 367

The following BLAST results are available for this feature:

BLAST of Carg10227 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013349.1	2.0e-29	100.00	Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyros...	[more]
KAG7026034.1	9.1e-27	89.86	Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022999826.1	1.2e-26	91.18	peroxisome biogenesis protein 16 isoform X1 [Cucurbita maxima]	[more]
XP_022999828.1	1.2e-26	91.18	peroxisome biogenesis protein 16 isoform X3 [Cucurbita maxima]	[more]
XP_022959429.1	2.6e-26	98.44	peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg10227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8S1	3.2e-19	67.19	Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 S...	[more]

BLAST of Carg10227 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KE71	5.7e-27	91.18	Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 P...	[more]
A0A6J1KKT7	5.7e-27	91.18	Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111494182 P...	[more]
A0A6J1H4U6	1.3e-26	98.44	Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410...	[more]
A0A6J1H817	1.3e-26	98.44	Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410...	[more]
A0A5D3DLT0	1.7e-26	96.88	Peroxisomal membrane protein PEX16 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Carg10227 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45690.1	2.3e-20	67.19	shrunken seed protein (SSE1)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013919	Peroxisome membrane protein, Pex16	PFAM	PF08610	Pex16	coord: 6..60 e-value: 7.1E-14 score: 51.8
IPR013919	Peroxisome membrane protein, Pex16	PANTHER	PTHR13299	UNCHARACTERIZED	coord: 6..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10227-RA	Carg10227-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007031	peroxisome organization
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005778	peroxisomal membrane