Carg10226 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10226
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Peroxisomal membrane protein PEX16
Location	Carg_Chr17: 31720 .. 34877 (-)
RNA-Seq Expression	Carg10226
Synteny	Carg10226

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTTCGCAATGATAATTGACGTTTGGTTTTAGTTCGTGTTTCTCTCTCCATCCAGATGCAGCCAAGTTTGTAAGGCAGTATGGAGGGTTTTCCAGATTGGCTGCTGTAATTGGGGTTCTGCAAAATTCCGGTTTCCCCTTCCCATAAATCCCAATTTGGTTCCTCCTTCTCTTCGCGCTTCTTCTCCTACAATTTCTCCCTTCATCAGCTACCTTTTCGTTTCTAGTTTCCTGAAAAGTTCACGAGCAGGATCCCTCTTTTTGAATGACCGTACCGTTGTGTTCTTAGATCCAATTTTTCTTTCGCCTGGCTATGAGGAGGGTTCTTCCTTGTTGCATCTTAGGGTTTTAGTTTGTACCAGGTTCTTGGACGATTCGAGTGTTTCTTTCTTTTGTTGCCGCTGCGTTGTGATTCTTTCAAGTCATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGTGAGCACTATAGATTTCTTATCATTACCCGCTGGTTTCTCTATTTGATTTCATCTGGGAATTTATTTGGTTGACTTGTTTGTTCTTTAATCTGTACGTAAACGGTGCTGCCCTTGGCTTTTATGGCGTTCTCTATTAAGTAATATTAGCATCTTTTTGCTAGTAGTTTATTTAAAGAACGGCTTTATGTTTCCACAGTTTATTTTCTGCAAATTTAAGATGGCATGCATGTCATGATGTTGATTTGATTTTTAAAAGAATAAGAGATATAGACAACAGATGGTATCCATATGTCTGATAGCAATGAGCATGAAGAGACCATTAAGTCAATTTATTCCTTGAAAATGAAGATTGATATACTATGGTCATTTTTTGTGAGTTTTGAATGTTCAACACTAGTCGATCGCCCGGCATTTTTATTGAAATCAGTTTGGGTAAACTGCACCTAAGTGCGTTTAATGTTATTAACATGTTCATTCTACATGATTATGTTCCTGTATGAGAGTACCCGCAAACTTTCTTATATACCATGAAACATATCGTCATTTGGTTATATGGATCTTATTATATTTTGATGTTTTTGATGGTCTGTGTATTGTTTAGATATTGAAATTGTAACAGCCTAAGCCTATCGCTAGCCGATATTGTCTTTTTTGGACTTTCCCTTTTGGGTTTCCCTTCAAGGTTTTAAAACGTGTCTGGTAGGGATAGATTTCCACACTCTTGTAAAGAATGCTTCGTTCTCCTCTTCAACCAATGTGAGATCCCACTATCCACCCTCCTTTGGGGCCCAACATCCTTGTTGGCACTCGTTCGGATTGTGAGATCCCACGTCGGTTGGAGAGGGGAACAAAACATTCTTTATAAGGGTGTAAAAACTTCTCCCTAACATATGTGTTTTAAAACCTTGAGGGGAAACCCCGAAGGGAAAACCCAAAAAGGACAACATCTGTTATCGGTGGGCTTGGGCTGTTATAGAAATATAGAAAATACCGAACTATTTGCATGTCGGTGAATGAGCCTTTTTCACTGTTTGATCACAAGCAATTGTAACCCCCCTTGTAATAGATCTTTTACTGCTTGCTTGTGTTGGCCTCATTATAATGCGGAACAACCCATTCAAATTACCTTGCAATGAGAGAAATAGAGTAGATTGGCTGAAAAGTTGAACCACCTTACTTTGACAGACATGTTTAATCGATATTATTAGTACATAATCTCCTCTAGAGGTTAATCTTACGGCAATTGTAGAACAATTTTTTTGCCATTTGAAAGTAGAACTGGGATGGTGGAATCTTGTTCAAGGATCATGGAAATATCAACGTTGGATGGGTTTCCATGTGCTAATTTGGTGCATGATGTATATTGTCATCTCATGTTTGTTTGTCATAAAACAGGTTAATTTCTCGATAGTTTTTGAAAACTGTTATATTACCATGATTAAAGTCAACTGTTAGTAGATCTGAGAGTATAAGTTTGTGGATTTCTACCTTTTATATGGTTCTTAATACTGTACAGGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGGTTTTGTGAAACTTTGGTAACACTTGACTGCTTCTTCTGAGCTTGGAATTTGTAGTGCCCTTGGGTCCCCTTTGATGAATATGCATGTCTTCATATTTGTCTCACTTTCAAATGCTATAAATTTACAGTAGACCATATCAAAAGCATTCTGATAACCAGCAAGGTTTTCTTAAGTTATTCTGTTTTCATTTTTCAACTTAGGGGTTCGAACCTCGTAACTTTGGAAGCTATCTCCCTACGTTCTAAGTTTGTACCAACAAACGTGAAAATTTAACTGCTATTTTGCTGTTTACTGGCGTTGTCTTGTCAAATGAACACGTTCCTGGCTTCTTTGTTCTAAAGTTTTGCCTTGCTCTGAGTGGATGATTTGCAACTTTGAAGCTATTATTAATTAGTTTAGGGATTTCTGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGTAAGACACAGTTGATGAGTGTGGGAGTGCCATTTTTTAATCTTCTGATACATTTTTATTGCATAGTCTTTGCCTTTACATTAGTAGCCGTTGAATTTCAGGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCCTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGGTGCCTATATCGATCCCAAGTTCCTAGAGTTTTGTGGGGGGAATGTCTACTGA

mRNA sequence

TTTGTTCGCAATGATAATTGACGTTTGGTTTTAGTTCGTGTTTCTCTCTCCATCCAGATGCAGCCAAGTTTGTAAGGCAGTATGGAGGGTTTTCCAGATTGGCTGCTGTAATTGGGGTTCTGCAAAATTCCGGTTTCCCCTTCCCATAAATCCCAATTTGGTTCCTCCTTCTCTTCGCGCTTCTTCTCCTACAATTTCTCCCTTCATCAGCTACCTTTTCGTTTCTAGTTTCCTGAAAAGTTCACGAGCAGGATCCCTCTTTTTGAATGACCGTACCGTTGTGTTCTTAGATCCAATTTTTCTTTCGCCTGGCTATGAGGAGGGTTCTTCCTTGTTGCATCTTAGGGTTTTAGTTTGTACCAGGTTCTTGGACGATTCGAGTGTTTCTTTCTTTTGTTGCCGCTGCGTTGTGATTCTTTCAAGTCATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCCTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGGTGCCTATATCGATCCCAAGTTCCTAGAGTTTTGTGGGGGGAATGTCTACTGA

Coding sequence (CDS)

ATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCCTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGGTGCCTATATCGATCCCAAGTTCCTAGAGTTTTGTGGGGGGAATGTCTACTGA

Protein sequence

MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRLALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLEGRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSGAYIDPKFLEFCGGNVY

Homology

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BLAST of Carg10226 vs. NCBI nr
Match: KAG7013350.1 (Peroxisome biogenesis protein 16 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 485.7 bits (1249), Expect = 2.2e-133
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSGAYIDPKFLEFCGGNVY 234
           GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSGAYIDPKFLEFCGGNVY
Sbjct: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSGAYIDPKFLEFCGGNVY 233

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BLAST of Carg10226 vs. NCBI nr
Match: KAG6574772.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 445.7 bits (1145), Expect = 2.5e-121
Identity = 215/216 (99.54%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 216

[Close]

BLAST of Carg10226 vs. NCBI nr
Match: XP_022959429.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 443.7 bits (1140), Expect = 9.6e-121
Identity = 215/216 (99.54%), Postives = 215/216 (99.54%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGEKARMIS PAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGEKARMISAPAWSYGVQHQHGMFNPS 216

[Close]

BLAST of Carg10226 vs. NCBI nr
Match: XP_023006235.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 441.4 bits (1134), Expect = 4.8e-120
Identity = 213/216 (98.61%), Postives = 213/216 (98.61%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKW RDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWARDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHD WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPS 216

[Close]

BLAST of Carg10226 vs. NCBI nr
Match: XP_023547844.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.0 bits (1125), Expect = 5.3e-119
Identity = 212/216 (98.15%), Postives = 212/216 (98.15%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGY GYLNG D WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYPGYLNGQDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPS 216

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BLAST of Carg10226 vs. ExPASy Swiss-Prot
Match: Q8S8S1 (Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-56
Identity = 120/220 (54.55%), Postives = 153/220 (69.55%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYK+WV  NR+Y+ SF S ANGLTWLLPE+FS SEIGPEAV+A +GI + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APT-QMHVNAT-VPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLF 120
           APT + HV ++    S  Y L I+ LK+LET++EVAA+ +YG DK+WN+I +TEA+K + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYS-GYLNGHDQW 180
           RLALF+N GYK+LL GGET N E+    S SQ+R      + G N   +  G  N H+ W
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAG----NSGRNLGPHGLGNQNHHNPW 180

Query: 181 NLEGRALSALSRFGEKARMI--SPPAWSYGVQHQHGMFNP 216
           NLEGRA+SALS FG+ AR    S P WS  +QHQ  +  P
Sbjct: 181 NLEGRAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEP 215

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BLAST of Carg10226 vs. ExPASy Swiss-Prot
Match: Q4QRH7 (Peroxisomal membrane protein PEX16 OS=Danio rerio OX=7955 GN=pex16 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-06
Identity = 35/124 (28.23%), Postives = 63/124 (50.81%), Query Frame = 0


Query: 2   EAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETA 61
           E Y+++VR +       ES    L++L+  RFSDS    E V +A  ++  +N+ I+   
Sbjct: 9   ERYQEYVRTSPAAASHLESTVRALSYLIAGRFSDSHEISELVYSASNLLVLLNDGIL--- 68

Query: 62  PTQMHVNATVPSSFPYS---LCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLF 121
             + +++ T+P S         +S L+ +E  +E+AA + +GD  RW  I + +  K + 
Sbjct: 69  --RKNLSRTLPMSISQQKLLTWLSVLEYVEVFVEMAAAKMWGDAGRWLVIVLIQIAKAVL 127

Query: 122 RLAL 123
           R  L
Sbjct: 129 RCLL 127

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BLAST of Carg10226 vs. ExPASy Swiss-Prot
Match: Q91XC9 (Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 3.4e-04
Identity = 32/122 (26.23%), Postives = 60/122 (49.18%), Query Frame = 0


Query: 4   YKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPT 63
           Y+++V  +       E+   GL++LL  RFSDS    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGLSYLLAGRFSDSHELSELVYSASNLLVLLNDGIL----- 70

Query: 64  QMHVNATVPSSFPYS---LCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 123
           +  +   +P S         +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+
Sbjct: 71  RKELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALIQLAKAVLRM 127

[Close]

BLAST of Carg10226 vs. ExPASy TrEMBL
Match: A0A6J1H817 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 4.6e-121
Identity = 215/216 (99.54%), Postives = 215/216 (99.54%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGEKARMIS PAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGEKARMISAPAWSYGVQHQHGMFNPS 216

[Close]

BLAST of Carg10226 vs. ExPASy TrEMBL
Match: A0A6J1KZL2 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111499027 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.3e-120
Identity = 213/216 (98.61%), Postives = 213/216 (98.61%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKW RDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWARDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHD WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPS 217
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPS
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPS 216

[Close]

BLAST of Carg10226 vs. ExPASy TrEMBL
Match: A0A1S3CCB9 (Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.8e-109
Identity = 193/215 (89.77%), Postives = 207/215 (96.28%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAY+KWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYRKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNAT+PSSFPYSLCI+ALKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRL
Sbjct: 61  APTQMHVNATMPSSFPYSLCIAALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYKILL+GGET NNERHL+TSTSQH+ NAFTKHGGH+ASG+SGYLNGH+ WNLE
Sbjct: 121 ALFKNSGYKILLEGGETTNNERHLETSTSQHKANAFTKHGGHHASGFSGYLNGHNPWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNP 216
           GRALSALSRFGE A+MISPPAWS+ VQHQH + NP
Sbjct: 181 GRALSALSRFGENAQMISPPAWSHRVQHQHAILNP 215

[Close]

BLAST of Carg10226 vs. ExPASy TrEMBL
Match: A0A0A0K8X7 (Peroxisomal membrane protein PEX16 OS=Cucumis sativus OX=3659 GN=Csa_6G075160 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.4e-108
Identity = 193/215 (89.77%), Postives = 203/215 (94.42%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAY+KWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYRKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYKILL+GGET N+ERHL+TSTS H+ NAFTKHGGH+ASG+SG LNGH  WNLE
Sbjct: 121 ALFKNSGYKILLEGGETTNSERHLETSTSHHKANAFTKHGGHHASGFSGDLNGHSPWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNP 216
           GRALSALSRFGE ARM SPPAWSY VQHQH + NP
Sbjct: 181 GRALSALSRFGENARMTSPPAWSYRVQHQHAILNP 215

[Close]

BLAST of Carg10226 vs. ExPASy TrEMBL
Match: A0A6J1DGN7 (Peroxisomal membrane protein PEX16 OS=Momordica charantia OX=3673 GN=LOC111020316 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 7.0e-101
Identity = 183/215 (85.12%), Postives = 194/215 (90.23%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNR YLHS ESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRHYLHSLESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
            PTQMHVN+  PSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIA TEA+KVLFRL
Sbjct: 61  TPTQMHVNSAGPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAATEAMKVLFRL 120

Query: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYK+LL+GGET NNE+HL TSTSQH+ NAFT HGG +ASGYS YLNGH+QWNLE
Sbjct: 121 ALFKNRGYKMLLEGGETPNNEKHLATSTSQHKANAFTNHGGGHASGYSRYLNGHNQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNP 216
           GRALSALSRFGE A +IS P WSY VQHQ+ M  P
Sbjct: 181 GRALSALSRFGENAPLISSPTWSYQVQHQNAMLKP 215

[Close]

BLAST of Carg10226 vs. TAIR 10
Match: AT2G45690.1 (shrunken seed protein (SSE1) )

HSP 1 Score: 221.1 bits (562), Expect = 9.4e-58
Identity = 120/220 (54.55%), Postives = 153/220 (69.55%), Query Frame = 0


Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYK+WV  NR+Y+ SF S ANGLTWLLPE+FS SEIGPEAV+A +GI + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APT-QMHVNAT-VPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLF 120
           APT + HV ++    S  Y L I+ LK+LET++EVAA+ +YG DK+WN+I +TEA+K + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYS-GYLNGHDQW 180
           RLALF+N GYK+LL GGET N E+    S SQ+R      + G N   +  G  N H+ W
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAG----NSGRNLGPHGLGNQNHHNPW 180

Query: 181 NLEGRALSALSRFGEKARMI--SPPAWSYGVQHQHGMFNP 216
           NLEGRA+SALS FG+ AR    S P WS  +QHQ  +  P
Sbjct: 181 NLEGRAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEP 215

The following BLAST results are available for this feature:

BLAST of Carg10226 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013350.1	2.2e-133	100.00	Peroxisome biogenesis protein 16 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6574772.1	2.5e-121	99.54	Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022959429.1	9.6e-121	99.54	peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata]	[more]
XP_023006235.1	4.8e-120	98.61	peroxisome biogenesis protein 16-like isoform X1 [Cucurbita maxima]	[more]
XP_023547844.1	5.3e-119	98.15	peroxisome biogenesis protein 16-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg10226 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8S1	1.3e-56	54.55	Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 S...	[more]
Q4QRH7	1.6e-06	28.23	Peroxisomal membrane protein PEX16 OS=Danio rerio OX=7955 GN=pex16 PE=2 SV=1	[more]
Q91XC9	3.4e-04	26.23	Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2	[more]

BLAST of Carg10226 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H817	4.6e-121	99.54	Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410...	[more]
A0A6J1KZL2	2.3e-120	98.61	Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111499027 P...	[more]
A0A1S3CCB9	1.8e-109	89.77	Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 ...	[more]
A0A0A0K8X7	3.4e-108	89.77	Peroxisomal membrane protein PEX16 OS=Cucumis sativus OX=3659 GN=Csa_6G075160 PE...	[more]
A0A6J1DGN7	7.0e-101	85.12	Peroxisomal membrane protein PEX16 OS=Momordica charantia OX=3673 GN=LOC11102031...	[more]

BLAST of Carg10226 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45690.1	9.4e-58	54.55	shrunken seed protein (SSE1)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013919	Peroxisome membrane protein, Pex16	PFAM	PF08610	Pex16	coord: 2..194 e-value: 4.9E-37 score: 128.0
IPR013919	Peroxisome membrane protein, Pex16	PANTHER	PTHR13299	UNCHARACTERIZED	coord: 1..215
None	No IPR available	PANTHER	PTHR13299:SF0	PEROXISOMAL MEMBRANE PROTEIN PEX16	coord: 1..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10226-RA	Carg10226-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007031	peroxisome organization
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005778	peroxisomal membrane