Carg09988 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09988
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionReticulon-like protein
LocationCarg_Chr10: 2195616 .. 2199287 (+)
RNA-Seq ExpressionCarg09988
SyntenyCarg09988
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAGAAAAGCAGGAAAAAAAAGGAAAAAAAAGAAAGGAAAAGAAAGGATAAAGAGAAACCCATCTCTCTTGAAACACAATTTTCCCCCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTCTCTGTCTTTCTTACTCTGCTTTTCAAAGGTATCCCAATTTCCGCCCCACCGACCGCCATTTCTTCATATTAAAGCTTCATATCCATCAATGTCACTGTAATTACTAGAAAGAGAAAAAGTGGGTTGGTGATAAAAAGAGGGAGATGGATTTGGGTAGAAGAAAAACAGGGGTTAGTAGTAGTGTGGTTTCAGGGTCTGTTTGGGAGAGCAGAATGAAGATGGATGAAGTGAAAGGTGGGATTAAGGTGTTCAATGGGGAAGATGGTAGTAACAAAGAAGGTAGAGCTGTAACAAGGGCAATGCCCAAGAGAACTACTACAGCTACTGATACTTCCGTAGTGAGTGCAAAGAGAAAGGTGTTGAGTTCAGACGGTTCAGATGGATCTGAAAAATCGCCAATTCAGAGAAGCCCAAATCAGCTGAGGAAGTTGAGATCTGAAGAAATTGAGAGAAGCCCAAATCAAATAAGGAAGTCAAAATCTGAAGTGGGGAGTAGATCAAGTGTTGAGAGAAATGAAAAAACCCCAATTCAGCTCAGGAAGGTGAAATCGGATTCCATCAGGACAATCGTCGACGACAATGCTAAGGCCATTGTTGTTGTCGGTGGAAGAAATGGGACAGATTTAGAGCATCTACAAAATCTAGAATCAGAATCGAACGAGAATTTGGGCGTAATTGAGAAGGGAATCCACGGATCTGTCAAGGAAATTGAGAAACCTCAAGTTGAGAATGAGACTTCTTGTAAGGAATTTGAAGTGTGCCACATTTCAACCATTGCGGGTCCGATCAAATCCACTCAGAAAGTGTCGATCAATGACAGTGATGTTAAAGATGAAAATGAAGCCAACGAAGAGGAGGAAACAGATGAAGAAGAAGAGGACATTGAAATTGAGAAGGAAAGCTTCGATATCAAGGAGATCAATTTACCAGAACCCAAAAAAGTTGTGAGTGAAGTGAAGCGATTCCACCAAATCTACGAAAAACCAGGTCCAACGTCCACCAAACCCACTTTCAGTAAGTAAAATCTTCTGCCATTTCATCCACTATTCTTTCACTAAATCAGTTCTTGTTCTTGAACATTCTGCTGTATAATTCTCAGCATCGAACGAATATCACAGCTTCCAAGAAAAAAACACCAAATTGCAGAGCTTAGGTACACTGAATTCAGGATCCTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGATTAATGGGTCTCTAGATTTCTCAACCCGAATTGTTCTTACTTTTTGGTCAGTGGACTTAGTAATGTGGAAGGAAGTATCGAGATCTGCCTTCGTGTTTGGGATTGGATCTTTTCTTATAATATCATCTTCCTACGCTAAGGACATTAACATCAGGTGAGAAAAAAAAAACCCCCCCTCTGGGTGGCTTTGAAAATTTACATTCAAACCCAAATTTCCATATATAATCAAATCTAAAGTGCTCTGTTTTTCTCTGTTCGAGTTGCAGCTTTATCTCTGTGATGTCATATGTGGGACTCTGCTACCTTGCTGCCACGTTCCTGTATAGATCCATTATTTGCAGGTAATCAATGTTCTCATGAGAATTGGTTGTGACTTGTGAGTGAATAAAGAGAAGAAGAAGAAGAAAGAAGAATGTAAGAGTTTTGTCTGTACTTGTTTGTTTAATGGGGATCACAAAATTCAGGGGAGCCATTGATATGGAGAACGTAAGGCATGTGGTTGGCGAAGAAGAAGCAATTTGGGCTCTTAAATTACTACTCCCTTACTTGAATGAGTTCTTATTAAAGCTCAGAGCTCTGTTTTGTGGAGATCCTGCAACTACAATCAAGGTATCTTTTTTTTTTCTTTTTCCCCCCTTTAATTTTTATTTAAATTATTATAATTTTTAAGAAGGTACGGAAAATAATGGTTGATTTATTTGCAAACATGCAGCTGGCTGTTTTCCTGTTTGCCTTGGCCCGATGTGGCAGCTCAATAACCGTCTGGAAAGTCGCCAAATGGGGTGAGATTTAAAATTTCCATTAAAATTTCCTTTTCAATTTTTTTTTTCCTTTTAATTCCTAATTTGTTAATTATATTTTCACACATTCAAATTCTAAAAAATATTTTTAAAAGTTATTTTTTTTAGGCTTTTTAAATTAACTTATTTTTTTTAAAACATTAGTAAAAATAAAAATTTAAAGGTAAAGGTAGTGTTTAACTTTTAAAAGCTAAAAATAATAAATTAAACGTAGTCCATAGATACAATAAATAATTCATACTTAGTTATTATTATTGTTAAATTATAAATGTAGTCTTTGAAATTCTGGGATATGTGTTTATATACCTAAACTTTCGATATATGTGTTTATTTTGGTATATGTGTTATTATTGAAACTCTAAATATATTGTCTTAACTCCGTATTTAATCATGTATGTGGTAGAATGTATTAAAGATATAAATTTGAGGCGCCTATTTCACGTTGTGGTAATTAAGAATGTGAAGAATAAAGTGAATTTGGATTGACGAGGTAACTTATATTTGGTGTCAGGTTTCTTCGGAGTATTTACCGTACCGAAAGTCTGCTCTTCGTATTCCTCGCAGCTGGCTGCTTACGGTAAAATCTCTGACCCAAAATGTCACTTTTGTCCCTTTCACGATACCGTTACTTACGTTCCCAATATCACTCTTCACCTGCTTTTCGTACTTTTGTCTCTCCCCGTCCCTGCTCCACCCCTAAACTATTTAACATTTTAATTTTATTATTTTTTTACCCGCATCAATACAATGTGAAAATCATGGAGGAAATTAATAAAAATGTTAAAATAAAAAGTTAAATGGTTATGGAATTTAAATTTATTATTAATTTTTAAAAATTAATTTCCATCTTGCTTTGTTTACAATTTGCAAATGGCCTAGCCATATTCTATTGTGGATCTAAAAGATCTCGGCACTCTTTTAAGGTGGGCCCATCGGCCCAAAAGACATAATAGCTACACACCCAATATTGGGCCTTGATACTTGTCCAGTTTGGGCCCAATTACAATATTTAAACGGAATTAACATATTGTTGGGCCATTGCAGCTAAATTTTGGGTTCGACGATTTCGTGATGCATGGGAGTCATGTTCTCACAAAAAGGGAGTGGCTGTAGCAATCTTCGTACTCGTATGGAACTTGTCTTCAGTGGTCGCTCGAGTTTGGGCTGGTAATTACTTCTTACTTCTTTAATCATCGGTCGGGTAGACTGATCAAAACTATAATATCTTAATCAATTGAGTTGAGTCGGTCGGGAAGATTGATCTAAAAGATGTATATATTTATTTAATTTCTTAAAAGATATTTATAAAATTAGGGATTTGTGTTATTTTGTTTCAGCATTTGTGCTGTTTGTGGCGTTTCGATATTACCAGCAATCTTTACAAACAGAGGAATGGGAGGAGGAGGAGGAGGAGGAGGTGGAAGAAGGTGAAGCAACGTGTCAAGTTTCTATTGGAAAGGGAAAATATGGGAGTGGGACCCTTTTTGGAGGTATGAGCCGTGGGAAACAAAAGAAGAAATTTTGA

mRNA sequence

GAAAAGAAAAGCAGGAAAAAAAAGGAAAAAAAAGAAAGGAAAAGAAAGGATAAAGAGAAACCCATCTCTCTTGAAACACAATTTTCCCCCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTCTCTGTCTTTCTTACTCTGCTTTTCAAAGGTATCCCAATTTCCGCCCCACCGACCGCCATTTCTTCATATTAAAGCTTCATATCCATCAATGTCACTGTAATTACTAGAAAGAGAAAAAGTGGGTTGGTGATAAAAAGAGGGAGATGGATTTGGGTAGAAGAAAAACAGGGGTTAGTAGTAGTGTGGTTTCAGGGTCTGTTTGGGAGAGCAGAATGAAGATGGATGAAGTGAAAGGTGGGATTAAGGTGTTCAATGGGGAAGATGGTAGTAACAAAGAAGGTAGAGCTGTAACAAGGGCAATGCCCAAGAGAACTACTACAGCTACTGATACTTCCGTAGTGAGTGCAAAGAGAAAGGTGTTGAGTTCAGACGGTTCAGATGGATCTGAAAAATCGCCAATTCAGAGAAGCCCAAATCAGCTGAGGAAGTTGAGATCTGAAGAAATTGAGAGAAGCCCAAATCAAATAAGGAAGTCAAAATCTGAAGTGGGGAGTAGATCAAGTGTTGAGAGAAATGAAAAAACCCCAATTCAGCTCAGGAAGGTGAAATCGGATTCCATCAGGACAATCGTCGACGACAATGCTAAGGCCATTGTTGTTGTCGGTGGAAGAAATGGGACAGATTTAGAGCATCTACAAAATCTAGAATCAGAATCGAACGAGAATTTGGGCGTAATTGAGAAGGGAATCCACGGATCTGTCAAGGAAATTGAGAAACCTCAAGTTGAGAATGAGACTTCTTGTAAGGAATTTGAAGTGTGCCACATTTCAACCATTGCGGGTCCGATCAAATCCACTCAGAAAGTGTCGATCAATGACAGTGATGTTAAAGATGAAAATGAAGCCAACGAAGAGGAGGAAACAGATGAAGAAGAAGAGGACATTGAAATTGAGAAGGAAAGCTTCGATATCAAGGAGATCAATTTACCAGAACCCAAAAAAGTTGTGAGTGAAGTGAAGCGATTCCACCAAATCTACGAAAAACCAGGTCCAACGTCCACCAAACCCACTTTCACATCGAACGAATATCACAGCTTCCAAGAAAAAAACACCAAATTGCAGAGCTTAGATTTCTCAACCCGAATTGTTCTTACTTTTTGGTCAGTGGACTTAGTAATGTGGAAGGAAGTATCGAGATCTGCCTTCGTGTTTGGGATTGGATCTTTTCTTATAATATCATCTTCCTACGCTAAGGACATTAACATCAGCTTTATCTCTGTGATGTCATATGTGGGACTCTGCTACCTTGCTGCCACGTTCCTGTATAGATCCATTATTTGCAGGGGAGCCATTGATATGGAGAACGTAAGGCATGTGGTTGGCGAAGAAGAAGCAATTTGGGCTCTTAAATTACTACTCCCTTACTTGAATGAGTTCTTATTAAAGCTCAGAGCTCTGTTTTGTGGAGATCCTGCAACTACAATCAAGCTGGCTGTTTTCCTGTTTGCCTTGGCCCGATGTGGCAGCTCAATAACCGTCTGGAAAGTCGCCAAATGGGGTTTCTTCGGAGTATTTACCGTACCGAAAGTCTGCTCTTCGTATTCCTCGCAGCTGGCTGCTTACGCTAAATTTTGGGTTCGACGATTTCGTGATGCATGGGAGTCATGTTCTCACAAAAAGGGAGTGGCTGTAGCAATCTTCGTACTCGTATGGAACTTGTCTTCAGTGGTCGCTCGAGTTTGGGCTGCATTTGTGCTGTTTGTGGCGTTTCGATATTACCAGCAATCTTTACAAACAGAGGAATGGGAGGAGGAGGAGGAGGAGGAGGTGGAAGAAGGTGAAGCAACGTGTCAAGTTTCTATTGGAAAGGGAAAATATGGGAGTGGGACCCTTTTTGGAGGTATGAGCCGTGGGAAACAAAAGAAGAAATTTTGA

Coding sequence (CDS)

ATGGATTTGGGTAGAAGAAAAACAGGGGTTAGTAGTAGTGTGGTTTCAGGGTCTGTTTGGGAGAGCAGAATGAAGATGGATGAAGTGAAAGGTGGGATTAAGGTGTTCAATGGGGAAGATGGTAGTAACAAAGAAGGTAGAGCTGTAACAAGGGCAATGCCCAAGAGAACTACTACAGCTACTGATACTTCCGTAGTGAGTGCAAAGAGAAAGGTGTTGAGTTCAGACGGTTCAGATGGATCTGAAAAATCGCCAATTCAGAGAAGCCCAAATCAGCTGAGGAAGTTGAGATCTGAAGAAATTGAGAGAAGCCCAAATCAAATAAGGAAGTCAAAATCTGAAGTGGGGAGTAGATCAAGTGTTGAGAGAAATGAAAAAACCCCAATTCAGCTCAGGAAGGTGAAATCGGATTCCATCAGGACAATCGTCGACGACAATGCTAAGGCCATTGTTGTTGTCGGTGGAAGAAATGGGACAGATTTAGAGCATCTACAAAATCTAGAATCAGAATCGAACGAGAATTTGGGCGTAATTGAGAAGGGAATCCACGGATCTGTCAAGGAAATTGAGAAACCTCAAGTTGAGAATGAGACTTCTTGTAAGGAATTTGAAGTGTGCCACATTTCAACCATTGCGGGTCCGATCAAATCCACTCAGAAAGTGTCGATCAATGACAGTGATGTTAAAGATGAAAATGAAGCCAACGAAGAGGAGGAAACAGATGAAGAAGAAGAGGACATTGAAATTGAGAAGGAAAGCTTCGATATCAAGGAGATCAATTTACCAGAACCCAAAAAAGTTGTGAGTGAAGTGAAGCGATTCCACCAAATCTACGAAAAACCAGGTCCAACGTCCACCAAACCCACTTTCACATCGAACGAATATCACAGCTTCCAAGAAAAAAACACCAAATTGCAGAGCTTAGATTTCTCAACCCGAATTGTTCTTACTTTTTGGTCAGTGGACTTAGTAATGTGGAAGGAAGTATCGAGATCTGCCTTCGTGTTTGGGATTGGATCTTTTCTTATAATATCATCTTCCTACGCTAAGGACATTAACATCAGCTTTATCTCTGTGATGTCATATGTGGGACTCTGCTACCTTGCTGCCACGTTCCTGTATAGATCCATTATTTGCAGGGGAGCCATTGATATGGAGAACGTAAGGCATGTGGTTGGCGAAGAAGAAGCAATTTGGGCTCTTAAATTACTACTCCCTTACTTGAATGAGTTCTTATTAAAGCTCAGAGCTCTGTTTTGTGGAGATCCTGCAACTACAATCAAGCTGGCTGTTTTCCTGTTTGCCTTGGCCCGATGTGGCAGCTCAATAACCGTCTGGAAAGTCGCCAAATGGGGTTTCTTCGGAGTATTTACCGTACCGAAAGTCTGCTCTTCGTATTCCTCGCAGCTGGCTGCTTACGCTAAATTTTGGGTTCGACGATTTCGTGATGCATGGGAGTCATGTTCTCACAAAAAGGGAGTGGCTGTAGCAATCTTCGTACTCGTATGGAACTTGTCTTCAGTGGTCGCTCGAGTTTGGGCTGCATTTGTGCTGTTTGTGGCGTTTCGATATTACCAGCAATCTTTACAAACAGAGGAATGGGAGGAGGAGGAGGAGGAGGAGGTGGAAGAAGGTGAAGCAACGTGTCAAGTTTCTATTGGAAAGGGAAAATATGGGAGTGGGACCCTTTTTGGAGGTATGAGCCGTGGGAAACAAAAGAAGAAATTTTGA

Protein sequence

MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTATDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQEKNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEEEVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Homology
BLAST of Carg09988 vs. NCBI nr
Match: KAG7023451.1 (Reticulon-like protein B21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 576

BLAST of Carg09988 vs. NCBI nr
Match: KAG6589779.1 (Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.2e-302
Identity = 561/576 (97.40%), Postives = 562/576 (97.57%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAV RAMPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVIRAMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIE 
Sbjct: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEN 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 564

BLAST of Carg09988 vs. NCBI nr
Match: XP_023515635.1 (reticulon-like protein B21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1046.6 bits (2705), Expect = 8.0e-302
Identity = 562/577 (97.40%), Postives = 563/577 (97.57%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 -EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
            EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 VEVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 565

BLAST of Carg09988 vs. NCBI nr
Match: XP_022921916.1 (reticulon-like protein B21 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.2e-301
Identity = 559/576 (97.05%), Postives = 560/576 (97.22%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTR MPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRVMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSI TIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENL VIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSISTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLVVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 564

BLAST of Carg09988 vs. NCBI nr
Match: XP_022988563.1 (reticulon-like protein B21 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.2e-297
Identity = 556/576 (96.53%), Postives = 557/576 (96.70%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAM KRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDS RTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST KVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTLKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSY KDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYTKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           E EEGE TCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 E-EEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 563

BLAST of Carg09988 vs. ExPASy Swiss-Prot
Match: Q56X72 (Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 2.8e-79
Identity = 203/483 (42.03%), Postives = 291/483 (60.25%), Query Frame = 0

Query: 98  SEEIERSPN---QIRKSKSEVGSRSSVERNEK--TPIQLRKVKSDSIRTIVDDNAKAIVV 157
           S+  ++SP+     +K++SE     SVE  EK  TP +++K++S+   TIV         
Sbjct: 10  SDSNDKSPSVSVVAKKARSE-----SVEGIEKKTTPGRVKKIRSEVCTTIVK-------- 69

Query: 158 VGGRNGTDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIA 217
            G  +   L  + +L S ++E     +      V  IE  ++  E   KEF VC    ++
Sbjct: 70  AGEFDSVALRKVNSLPSPNSEK---SDTKTEQEVTIIENSKIPEEV--KEFGVCQEMIVS 129

Query: 218 GPIKSTQKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVS-EV 277
                 +++   D ++ D+   ++ EE  +EEE+ E+EK+S D+KEIN+ +  +V   E+
Sbjct: 130 AKSNENEQIDNGDQEIGDQ---DDYEEDGDEEEEREVEKKSVDVKEINVAKENRVGGVEI 189

Query: 278 KRFHQIYEKPGPT-------------------STKPTFTSN------EYHSFQEKNTKLQ 337
           K+F Q   +  P+                   S  P  TS+      +  +F    +KLQ
Sbjct: 190 KKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPNSTSSTDRFAEQEDNFTHSQSKLQ 249

Query: 338 SLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLC 397
           SL            VDLVMW++VSRS  VFG G+FLIISSSYA D+N SFISV++Y+GL 
Sbjct: 250 SL------------VDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLI 309

Query: 398 YLAATFLYRSIICRGAIDMENVRHV-VGEEEAIWALKLLLPYLNEFLLKLRALFCGDPAT 457
           YL   F+ +S+I RG ++ E  + V V EE+    L+L++PYLNE L +LRALF GDP+T
Sbjct: 310 YLGLMFVLKSLIHRGMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPST 369

Query: 458 TIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAW 517
           T+K+ V LF LARCGSSIT+W +AK+GF G FT+PK+  SYS+  +AY  FW+RRFRDAW
Sbjct: 370 TLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFWMRRFRDAW 429

Query: 518 ESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQ-QSLQTEEWEEEEEEEVEE 548
           ESC+HKK VA+A+F LVWNLSSV ARVWAAF+L VAFRYYQ + + T +  +++E++ EE
Sbjct: 430 ESCNHKKAVALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKMIWTTDQADDDEDDNEE 459

BLAST of Carg09988 vs. ExPASy Swiss-Prot
Match: Q6DR04 (Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.5e-32
Identity = 101/297 (34.01%), Postives = 161/297 (54.21%), Query Frame = 0

Query: 252 ESFDIKEINL--PEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQEKNTKLQSLD 311
           E+ + KE NL   E  K+    ++ +   +K   +S  P  +S++       NT    L+
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTNGRPKKDKQSSAPPLCSSSDL-----PNTCQSDLN 167

Query: 312 FSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLCYLA 371
               I+      DLVMW++V++S   FG G    +SS +AK +N S  S +S +GL  L 
Sbjct: 168 LIGEII-----SDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLC 227

Query: 372 ATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKL 431
            +FL  ++  R   D +   H V E++ + + + +LP  N F+ K   LF G+P+ T+K+
Sbjct: 228 GSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKV 287

Query: 432 AVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAWESCS 491
             FL   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++   +    R  +AW  CS
Sbjct: 288 TPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCS 347

Query: 492 HKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQ--TEEWEEEEEEEVEE 545
           HKK +A +     WNL+S+  R++A F++ V FRY +Q+LQ   EE E  E E+ EE
Sbjct: 348 HKKILAGSAVTAFWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEEVEPVENEQEEE 393

BLAST of Carg09988 vs. ExPASy Swiss-Prot
Match: Q8LDS3 (Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 4.3e-32
Identity = 84/225 (37.33%), Postives = 134/225 (59.56%), Query Frame = 0

Query: 322 DLVMWKEVSRSAFVFGIGSFLIISSSY-AKDINISFISVMSYVGLCYLAATFLYRSIICR 381
           DL+MW++V++S   FG G    +S+ + AK  N S  S +SY+GL +L  +FL  ++  R
Sbjct: 197 DLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLFLGVSFLSNTL--R 256

Query: 382 GAIDMENVRHV-VGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKLAVFLFALARC 441
             +  E  R + + E++ +   + +LP  N  + K   LF G+PA T+K+A F+   A  
Sbjct: 257 QRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMTLKVAPFVLMGAEY 316

Query: 442 GSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIF 501
           G  IT+W++  +GFF  FT+PK+ S Y+SQL    +   RRF +AW  C+HKK VA +  
Sbjct: 317 GYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKVECAQRRFVEAWGVCTHKKFVAGSAV 376

Query: 502 VLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEEEVEE 545
              WNL+S+  R  A F++ V  RY +Q+LQ +  +EEE+++ E+
Sbjct: 377 TAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLDSEDEEEKKQQEK 419

BLAST of Carg09988 vs. ExPASy TrEMBL
Match: A0A6J1E2P4 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430032 PE=4 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.5e-301
Identity = 559/576 (97.05%), Postives = 560/576 (97.22%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTR MPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRVMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSI TIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENL VIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSISTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLVVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 564

BLAST of Carg09988 vs. ExPASy TrEMBL
Match: A0A6J1JMN2 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1)

HSP 1 Score: 1032.7 bits (2669), Expect = 5.8e-298
Identity = 556/576 (96.53%), Postives = 557/576 (96.70%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAM KRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDS RTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST KVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTLKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSY KDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYTKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           E EEGE TCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 E-EEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 563

BLAST of Carg09988 vs. ExPASy TrEMBL
Match: A0A6J1E1Q6 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430032 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.5e-293
Identity = 549/576 (95.31%), Postives = 550/576 (95.49%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTR MPKRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRVMPKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDSI TIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENL VIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSISTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLVVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKP          SNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKP---------ASNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 555

BLAST of Carg09988 vs. ExPASy TrEMBL
Match: A0A6J1JLX7 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 546/576 (94.79%), Postives = 547/576 (94.97%), Query Frame = 0

Query: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTTA 60
           MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAM KRTTTA
Sbjct: 1   MDLGRRKTGVSSSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTTA 60

Query: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120
           TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS
Sbjct: 61  TDTSVVSAKRKVLSSDGSDGSEKSPIQRSPNQLRKLRSEEIERSPNQIRKSKSEVGSRSS 120

Query: 121 VERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180
           VERNEKTPIQLRKVKSDS RTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK
Sbjct: 121 VERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRNGTDLEHLQNLESESNENLGVIEK 180

Query: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSDVKDENEANEEEET 240
           GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST KVSINDSDVKDENEANEEEET
Sbjct: 181 GIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTLKVSINDSDVKDENEANEEEET 240

Query: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQE 300
           DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKP          SNEYHSFQE
Sbjct: 241 DEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKP---------ASNEYHSFQE 300

Query: 301 KNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVM 360
           K+TKLQSL            VDLVMWKEVSRSAFVFGIGSFLIISSSY KDINISFISVM
Sbjct: 301 KSTKLQSL------------VDLVMWKEVSRSAFVFGIGSFLIISSSYTKDINISFISVM 360

Query: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420
           SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC
Sbjct: 361 SYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFC 420

Query: 421 GDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480
           GDPATTIKLAV LFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR
Sbjct: 421 GDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRR 480

Query: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540
           FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE
Sbjct: 481 FRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEE 540

Query: 541 EVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 577
           E EEGE TCQVSIGKGKYGSGTLFGGMSRGKQKKKF
Sbjct: 541 E-EEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 554

BLAST of Carg09988 vs. ExPASy TrEMBL
Match: A0A5A7SUW8 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G001210 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 3.6e-231
Identity = 457/594 (76.94%), Postives = 497/594 (83.67%), Query Frame = 0

Query: 1   MDLGRRKTGV-SSSVVSGSVWESRMKMDEVKGGIKVFNGEDG-SNKEGRAVTRAMPKRTT 60
           MD+GRRK GV S+SVVSGSVWESRMKMDEVKGGIKVFNGEDG SNKEG+ VTR+M KR +
Sbjct: 1   MDVGRRKVGVGSNSVVSGSVWESRMKMDEVKGGIKVFNGEDGSSNKEGKVVTRSMSKRIS 60

Query: 61  TATDTSVVSAKRKVLSSDGSDGSEKSPIQ-----------RSPNQLRKLRSEEIERSPNQ 120
                               +GSEKSPIQ           +SP QLRK+RSEEIERSPNQ
Sbjct: 61  L-------------------EGSEKSPIQIGKGKRNSDDTKSPIQLRKMRSEEIERSPNQ 120

Query: 121 IRKSKSEVGSRSSVERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRNGTDLEHLQNL 180
           +RKSKSE+     VERNEKT IQLRKVKS+S++TIVDDN KAIVVVGG+NG + EHLQ  
Sbjct: 121 LRKSKSEL-----VERNEKTQIQLRKVKSESVKTIVDDNVKAIVVVGGKNGGNSEHLQCP 180

Query: 181 ESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKSTQKVSINDSD 240
           ESESNE L  IEKGIHGS+KEIEKPQVE ETSCKEF VCHIST+AGPIKSTQKVSIND  
Sbjct: 181 ESESNEELSEIEKGIHGSIKEIEKPQVEIETSCKEFGVCHISTMAGPIKSTQKVSIND-- 240

Query: 241 VKDENEANEEEETDEEEE---DIEIEKESFDIKEINLPEPKKVVSEVKRFHQIYEKPGPT 300
             +ENEANEEEET+EEEE   D EIEKESFDIKEINLPEPKKVV+EVK+FHQIYEKP P 
Sbjct: 241 --NENEANEEEETEEEEEDDDDNEIEKESFDIKEINLPEPKKVVNEVKKFHQIYEKPVPN 300

Query: 301 STKPTFTSNEYHSFQEKNTKLQSLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLII 360
           STKPT+TS EYH+FQEK  KLQSL            VDL+MWKEVSRSAFVFGIGSF+II
Sbjct: 301 STKPTYTSKEYHNFQEKGNKLQSL------------VDLIMWKEVSRSAFVFGIGSFVII 360

Query: 361 SSSYAKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLL 420
           SSSY  DINISFISV+SYVGL YLAATFLYRSIICRGAIDMENVR+VVGEEEAIW LKLL
Sbjct: 361 SSSYTNDINISFISVVSYVGLFYLAATFLYRSIICRGAIDMENVRNVVGEEEAIWVLKLL 420

Query: 421 LPYLNEFLLKLRALFCGDPATTIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCS 480
           LPYLNEFLLKLRALFCGDPATT+KLAV LFA ARCGSSITVWKVAKWGFFGVFT+PK+CS
Sbjct: 421 LPYLNEFLLKLRALFCGDPATTMKLAVLLFASARCGSSITVWKVAKWGFFGVFTLPKICS 480

Query: 481 SYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRY 540
           SYSS LAAYAKFWVRRFRDAWESCSHKKGVA+AIFVLVWNLSSVVARVWAAF+LFVAFRY
Sbjct: 481 SYSSHLAAYAKFWVRRFRDAWESCSHKKGVALAIFVLVWNLSSVVARVWAAFMLFVAFRY 540

Query: 541 YQQSLQTEE-WEEEEEEEVEEGEATCQVSIGKGKYGSGTLFGGM-SRGKQKKKF 577
           YQQS+QTEE W +E+++  EEG+ TCQ SIGK KYGS +LFGG+ S  KQKKKF
Sbjct: 541 YQQSIQTEEYWGDEDDQVEEEGDETCQASIGKEKYGSASLFGGIGSSAKQKKKF 554

BLAST of Carg09988 vs. TAIR 10
Match: AT5G58000.1 (Reticulon family protein )

HSP 1 Score: 297.7 bits (761), Expect = 2.0e-80
Identity = 203/483 (42.03%), Postives = 291/483 (60.25%), Query Frame = 0

Query: 98  SEEIERSPN---QIRKSKSEVGSRSSVERNEK--TPIQLRKVKSDSIRTIVDDNAKAIVV 157
           S+  ++SP+     +K++SE     SVE  EK  TP +++K++S+   TIV         
Sbjct: 10  SDSNDKSPSVSVVAKKARSE-----SVEGIEKKTTPGRVKKIRSEVCTTIVK-------- 69

Query: 158 VGGRNGTDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIA 217
            G  +   L  + +L S ++E     +      V  IE  ++  E   KEF VC    ++
Sbjct: 70  AGEFDSVALRKVNSLPSPNSEK---SDTKTEQEVTIIENSKIPEEV--KEFGVCQEMIVS 129

Query: 218 GPIKSTQKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVS-EV 277
                 +++   D ++ D+   ++ EE  +EEE+ E+EK+S D+KEIN+ +  +V   E+
Sbjct: 130 AKSNENEQIDNGDQEIGDQ---DDYEEDGDEEEEREVEKKSVDVKEINVAKENRVGGVEI 189

Query: 278 KRFHQIYEKPGPT-------------------STKPTFTSN------EYHSFQEKNTKLQ 337
           K+F Q   +  P+                   S  P  TS+      +  +F    +KLQ
Sbjct: 190 KKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPNSTSSTDRFAEQEDNFTHSQSKLQ 249

Query: 338 SLDFSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLC 397
           SL            VDLVMW++VSRS  VFG G+FLIISSSYA D+N SFISV++Y+GL 
Sbjct: 250 SL------------VDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLI 309

Query: 398 YLAATFLYRSIICRGAIDMENVRHV-VGEEEAIWALKLLLPYLNEFLLKLRALFCGDPAT 457
           YL   F+ +S+I RG ++ E  + V V EE+    L+L++PYLNE L +LRALF GDP+T
Sbjct: 310 YLGLMFVLKSLIHRGMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPST 369

Query: 458 TIKLAVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAW 517
           T+K+ V LF LARCGSSIT+W +AK+GF G FT+PK+  SYS+  +AY  FW+RRFRDAW
Sbjct: 370 TLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFWMRRFRDAW 429

Query: 518 ESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQ-QSLQTEEWEEEEEEEVEE 548
           ESC+HKK VA+A+F LVWNLSSV ARVWAAF+L VAFRYYQ + + T +  +++E++ EE
Sbjct: 430 ESCNHKKAVALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKMIWTTDQADDDEDDNEE 459

BLAST of Carg09988 vs. TAIR 10
Match: AT2G20590.1 (Reticulon family protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 101/297 (34.01%), Postives = 161/297 (54.21%), Query Frame = 0

Query: 252 ESFDIKEINL--PEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQEKNTKLQSLD 311
           E+ + KE NL   E  K+    ++ +   +K   +S  P  +S++       NT    L+
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTNGRPKKDKQSSAPPLCSSSDL-----PNTCQSDLN 167

Query: 312 FSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLCYLA 371
               I+      DLVMW++V++S   FG G    +SS +AK +N S  S +S +GL  L 
Sbjct: 168 LIGEII-----SDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLC 227

Query: 372 ATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKL 431
            +FL  ++  R   D +   H V E++ + + + +LP  N F+ K   LF G+P+ T+K+
Sbjct: 228 GSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKV 287

Query: 432 AVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAWESCS 491
             FL   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++   +    R  +AW  CS
Sbjct: 288 TPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCS 347

Query: 492 HKKGVAVAIFVLVWNLSSVVARVWAAFVLFVAFRYYQQSLQ--TEEWEEEEEEEVEE 545
           HKK +A +     WNL+S+  R++A F++ V FRY +Q+LQ   EE E  E E+ EE
Sbjct: 348 HKKILAGSAVTAFWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEEVEPVENEQEEE 393

BLAST of Carg09988 vs. TAIR 10
Match: AT4G28430.1 (Reticulon family protein )

HSP 1 Score: 141.0 bits (354), Expect = 3.1e-33
Identity = 84/225 (37.33%), Postives = 134/225 (59.56%), Query Frame = 0

Query: 322 DLVMWKEVSRSAFVFGIGSFLIISSSY-AKDINISFISVMSYVGLCYLAATFLYRSIICR 381
           DL+MW++V++S   FG G    +S+ + AK  N S  S +SY+GL +L  +FL  ++  R
Sbjct: 197 DLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLFLGVSFLSNTL--R 256

Query: 382 GAIDMENVRHV-VGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKLAVFLFALARC 441
             +  E  R + + E++ +   + +LP  N  + K   LF G+PA T+K+A F+   A  
Sbjct: 257 QRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMTLKVAPFVLMGAEY 316

Query: 442 GSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIF 501
           G  IT+W++  +GFF  FT+PK+ S Y+SQL    +   RRF +AW  C+HKK VA +  
Sbjct: 317 GYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKVECAQRRFVEAWGVCTHKKFVAGSAV 376

Query: 502 VLVWNLSSVVARVWAAFVLFVAFRYYQQSLQTEEWEEEEEEEVEE 545
              WNL+S+  R  A F++ V  RY +Q+LQ +  +EEE+++ E+
Sbjct: 377 TAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLDSEDEEEKKQQEK 419

BLAST of Carg09988 vs. TAIR 10
Match: AT2G20590.2 (Reticulon family protein )

HSP 1 Score: 100.1 bits (248), Expect = 6.0e-21
Identity = 71/222 (31.98%), Postives = 118/222 (53.15%), Query Frame = 0

Query: 252 ESFDIKEINL--PEPKKVVSEVKRFHQIYEKPGPTSTKPTFTSNEYHSFQEKNTKLQSLD 311
           E+ + KE NL   E  K+    ++ +   +K   +S  P  +S++       NT    L+
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTNGRPKKDKQSSAPPLCSSSDL-----PNTCQSDLN 167

Query: 312 FSTRIVLTFWSVDLVMWKEVSRSAFVFGIGSFLIISSSYAKDINISFISVMSYVGLCYLA 371
               I+      DLVMW++V++S   FG G    +SS +AK +N S  S +S +GL  L 
Sbjct: 168 LIGEII-----SDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLC 227

Query: 372 ATFLYRSIICRGAIDMENVRHVVGEEEAIWALKLLLPYLNEFLLKLRALFCGDPATTIKL 431
            +FL  ++  R   D +   H V E++ + + + +LP  N F+ K   LF G+P+ T+K+
Sbjct: 228 GSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKV 287

Query: 432 AVFLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 472
             FL   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++
Sbjct: 288 TPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQIS 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023451.10.0e+00100.00Reticulon-like protein B21 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589779.11.2e-30297.40Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023515635.18.0e-30297.40reticulon-like protein B21 [Cucurbita pepo subsp. pepo][more]
XP_022921916.15.2e-30197.05reticulon-like protein B21 isoform X1 [Cucurbita moschata][more]
XP_022988563.11.2e-29796.53reticulon-like protein B21 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q56X722.8e-7942.03Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2[more]
Q6DR041.5e-3234.01Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1[more]
Q8LDS34.3e-3237.33Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1E2P42.5e-30197.05Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430032 PE=4 SV=1[more]
A0A6J1JMN25.8e-29896.53Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1[more]
A0A6J1E1Q69.5e-29395.31Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430032 PE=4 SV=1[more]
A0A6J1JLX71.7e-28994.79Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1[more]
A0A5A7SUW83.6e-23176.94Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
Match NameE-valueIdentityDescription
AT5G58000.12.0e-8042.03Reticulon family protein [more]
AT2G20590.11.1e-3334.01Reticulon family protein [more]
AT4G28430.13.1e-3337.33Reticulon family protein [more]
AT2G20590.26.0e-2131.98Reticulon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 233..253
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..248
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..92
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..248
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 553..576
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..71
NoneNo IPR availablePANTHERPTHR46626:SF1RETICULON-LIKE PROTEIN B21coord: 1..563
IPR003388ReticulonPFAMPF02453Reticuloncoord: 321..475
e-value: 1.7E-21
score: 76.8
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 320..483
score: 12.500723
IPR044647Reticulon-like protein B17/18/21PANTHERPTHR46626RETICULON-LIKE PROTEIN B17coord: 1..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09988-RACarg09988-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane