Carg09974 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09974
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DAO domain-containing protein
Location	Carg_Chr10: 2272598 .. 2275217 (-)
RNA-Seq Expression	Carg09974
Synteny	Carg09974

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGCCGCGCCGTCGTTAGCACTTTCTTCTCTCTCGGCGCGTCCTCTGAGGTTCTTCTTCAACCTTTTCTTTACCTAATTAATGTGCGTTTCTGAGTTCCTTTGCGAAATGTATATGTATGTACTCAGAGAGGGGTATCCTTTTGTAGGTACGCAGTGCTTGGAGCTGGTTTTGCTGGCCTTTCGGTTACTTGGCATTTGTTGAAGGTACGCCGGCTCTGTGGCTTTCTTTTTCCTCTTCGTTTCATCATACATCAACACCTTGCTTTCGCTGCATACTCTGTTTTCTTCAATCTTTTTTCGGTTGTTGGACTGTGGTTTTTGTGTATTGAACTTTTGTGATACTCCATTACAAGGGTACGTGAATGAATCGATGACATATCCAAATGAGAACGATTTTAAAGAATTAATATCTATATTGACAAATGCACCTTACTTTTCGGTGACACAACCATAAAAATTTCAAAGTTAAAGTAGACTTGTTGCGCGACCTCCTGTATATTTTCTTGAGAAGCATATGAGAGAGGAGAAAACATGTTGAAAGAAACCGGTTCATTCTTAGAACTAAATAGGTTACTTGACATTGTCGCTAAAGGACATCAGGACCACAAGTGTCGAATCCAGATTTTGAATCTTAATCCAAATTCGGGGTAATTTGTAACCCTATGATGACATACACTCTCTTGAAATATGTTCCCTCATTTTGTCAGCCCTAAACCAAGGTCTATTGAACCTTGCGTAGCTATTGCATCTACATATTCTCTATGTAAATAACTGGACAGATTCCCGAGGTTCCTACTTCCTCTAATGCTTCAGGCGGATCGGTCTTGCTTCTGCTACCAATTAGTATACTTTGAGTTTTATCTCAAGGTTCTTTATCTTGCCAATGTGAGGCTTTAGTCGCATTCCAACCCTTGTTACTTAATGTTTCATTTCATCGTATTGTGTTTATTGCAGCTCTGTCCTAAGGACATATCTCTATGTATCGACATATACGACGAAGTTGGCATTGGTGGAGGAGCATCTGGAGTCGCCGGAGGGCTTCTTCACCCTTATTCTCCTAAAGGTTCCAACAACCTACTTAGTTACCCAATCTCCCTTGCAAAAATTGGCTAATCCCTTGAAATTATGTCAGTTAAACCTCTCTGGAAAGGTGCCGAGTGTTGGGCTGAGTGCTTAAAGCTTTTAAGCGTTGCGGAGTCAGCAATTCCCTCCAAAGAGTTGGATGGATATCATAATATGAATGGATCCGTTGTTCTAAGACGGTCTGCTACTTCATTTCTTTTAGCTTCTGAGAGATATATATACTCATTCCCATCACTTGACATGCTTCTGCTGTATTTTGTTTACTTCAGGGGTATCTTGAGGCCTGCAATCAGCTTGAAGAATCTCATGATGTTAAAAGAAGTAATTATATCCTTCTATTTGTGTTTCATTACTCTGATTAGAACGTCAAAACGACCTTGATAGTATCTTTGTTATTTACCCAGAATGCTCAGAATGGACTCGATGGCTGCAGATGCTGCCCAGAATCTTGTTCCTAAGCTTAACATTCCTCTAAATACAGCTTTTTATATGCCTCAAGCTGTAAATATCAACTCTCAACGTTATCTTGAGGTCACTACTTCAAAGCAAATGATACTTAAGTTGTTCTTCCATCTGTTATGATTAAAACAAATCTTTGATCTTTTTTGTTCCCAATCTTATGTATAGGCGCTGTTTATAGCATGTCAAAATCTGACCAGGGAAACATCACCCTCAAGTCTTGGTCATAAGGAGTTGTTCTTGCAGAAAAAGTCTATTAACGAACTGGGTGAACTAGAAGGTAAGTGAAATTTCTCAAGCCAAATCTTCTCAGCATTATCTTGGAATTAAACTGTTTAAAGAACATTCTGTGCAGGGACATACGATGCTGTGATTGTTTGTCTTGGTGCTAAAATGATCATGCTTCCTGAACTTACTGGAAAGCTTCCTTTGAGAACTTGCAGAGGTGTGGTAGCTCATTTTCAGCTTCCCGACGATATCAGGTATTGGTTTCTTGTGCCTAGTATGTATTATCCCTCCACAAAATTCATAATTTGCGCCCATGCGGGGGTCGAGATTTGAACTTCAAACCCCTGGATCCTGTTAGTCTGATATCTGGTATAATACTATAATTTCAGCAATGCATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTAGCCATACAGAGTCCTAGAAGTTTATATATGGGTTCAACATGGGAATGGAAATCAACAAATTCATCACCTGAAGTTTCAGAAGAGGAAGGCTCAAGGGCGGTTGCAGAACTCTTACCAAAGGTGTCTGCCATTTATCCCTCAATAAAGGAGTGGAGTTTCAGGAGAGCAAGGGCTGGTCTTAGAGCAATGCCACCACTCACTCCTCATGGATCTCTTCCTCTTATGGGTTGTATAGACGAGATTGTAAGACCTAACAGCTCTTGCAAGTACTGGTTATTTGGAGGGCTTGGTTCTAGGGGATTGTTGTACCATGGTTGGCTTGGGAAACTAACAGCACAGGCTGTGCTTTCATGTAAGGAAGAACTAATCCCATCTGAACTAATCTCTTGGAAGAAAGTAATGAATAGTTAA

mRNA sequence

CCGCCGCGCCGTCGTTAGCACTTTCTTCTCTCTCGGCGCGTCCTCTGAGGTACGCAGTGCTTGGAGCTGGTTTTGCTGGCCTTTCGGTTACTTGGCATTTGTTGAAGCTCTGTCCTAAGGACATATCTCTATGTATCGACATATACGACGAAGTTGGCATTGGTGGAGGAGCATCTGGAGTCGCCGGAGGGCTTCTTCACCCTTATTCTCCTAAAGTTAAACCTCTCTGGAAAGGTGCCGAGTGTTGGGCTGAGTGCTTAAAGCTTTTAAGCGTTGCGGAGTCAGCAATTCCCTCCAAAGAGTTGGATGGATATCATAATATGAATGGATCCGTTGTTCTAAGACGGTCTGCTACTTCATTTCTTTTAGCTTCTGAGAGATATATATACTCATTCCCATCACTTGACATGCTTCTGCTGTATTTTGTTTACTTCAGGGGTATCTTGAGGCCTGCAATCAGCTTGAAGAATCTCATGATGTTAAAAGAAGTAATTATATCCTTAATGCTCAGAATGGACTCGATGGCTGCAGATGCTGCCCAGAATCTTGTTCCTAAGCTTAACATTCCTCTAAATACAGCTTTTTATATGCCTCAAGCTGTAAATATCAACTCTCAACGTTATCTTGAGGCGCTGTTTATAGCATGTCAAAATCTGACCAGGGAAACATCACCCTCAAGTCTTGGTCATAAGGAGTTGTTCTTGCAGAAAAAGTCTATTAACGAACTGGGTGAACTAGAAGGGACATACGATGCTGTGATTGTTTGTCTTGGTGCTAAAATGATCATGCTTCCTGAACTTACTGGAAAGCTTCCTTTGAGAACTTGCAGAGGTGTGGTAGCTCATTTTCAGCTTCCCGACGATATCAGCAATGCATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTAGCCATACAGAGTCCTAGAAGTTTATATATGGGTTCAACATGGGAATGGAAATCAACAAATTCATCACCTGAAGTTTCAGAAGAGGAAGGCTCAAGGGCGGTTGCAGAACTCTTACCAAAGGTGTCTGCCATTTATCCCTCAATAAAGGAGTGGAGTTTCAGGAGAGCAAGGGCTGGTCTTAGAGCAATGCCACCACTCACTCCTCATGGATCTCTTCCTCTTATGGGTTGTATAGACGAGATTGTAAGACCTAACAGCTCTTGCAAGTACTGGTTATTTGGAGGGCTTGGTTCTAGGGGATTGTTGTACCATGGTTGGCTTGGGAAACTAACAGCACAGGCTGTGCTTTCATGTAAGGAAGAACTAATCCCATCTGAACTAATCTCTTGGAAGAAAGTAATGAATAGTTAA

Coding sequence (CDS)

ATGAATGGATCCGTTGTTCTAAGACGGTCTGCTACTTCATTTCTTTTAGCTTCTGAGAGATATATATACTCATTCCCATCACTTGACATGCTTCTGCTGTATTTTGTTTACTTCAGGGGTATCTTGAGGCCTGCAATCAGCTTGAAGAATCTCATGATGTTAAAAGAAGTAATTATATCCTTAATGCTCAGAATGGACTCGATGGCTGCAGATGCTGCCCAGAATCTTGTTCCTAAGCTTAACATTCCTCTAAATACAGCTTTTTATATGCCTCAAGCTGTAAATATCAACTCTCAACGTTATCTTGAGGCGCTGTTTATAGCATGTCAAAATCTGACCAGGGAAACATCACCCTCAAGTCTTGGTCATAAGGAGTTGTTCTTGCAGAAAAAGTCTATTAACGAACTGGGTGAACTAGAAGGGACATACGATGCTGTGATTGTTTGTCTTGGTGCTAAAATGATCATGCTTCCTGAACTTACTGGAAAGCTTCCTTTGAGAACTTGCAGAGGTGTGGTAGCTCATTTTCAGCTTCCCGACGATATCAGCAATGCATACCCAGAGCTTGGGCCTTCTATACTGTCAGATGCATGGCTAGCCATACAGAGTCCTAGAAGTTTATATATGGGTTCAACATGGGAATGGAAATCAACAAATTCATCACCTGAAGTTTCAGAAGAGGAAGGCTCAAGGGCGGTTGCAGAACTCTTACCAAAGGTGTCTGCCATTTATCCCTCAATAAAGGAGTGGAGTTTCAGGAGAGCAAGGGCTGGTCTTAGAGCAATGCCACCACTCACTCCTCATGGATCTCTTCCTCTTATGGGTTGTATAGACGAGATTGTAAGACCTAACAGCTCTTGCAAGTACTGGTTATTTGGAGGGCTTGGTTCTAGGGGATTGTTGTACCATGGTTGGCTTGGGAAACTAACAGCACAGGCTGTGCTTTCATGTAAGGAAGAACTAATCCCATCTGAACTAATCTCTTGGAAGAAAGTAATGAATAGTTAA

Protein sequence

MNGSVVLRRSATSFLLASERYIYSFPSLDMLLLYFVYFRGILRPAISLKNLMMLKEVIISLMLRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNINSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIMLPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWKSTNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGCIDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS

Homology

[Close]

BLAST of Carg09974 vs. NCBI nr
Match: KAG7023465.1 (hypothetical protein SDJN02_14490 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666.8 bits (1719), Expect = 1.0e-187
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0


Query: 1   MNGSVVLRRSATSFLLASERYIYSFPSLDMLLLYFVYFRGILRPAISLKNLMMLKEVIIS 60
           MNGSVVLRRSATSFLLASERYIYSFPSLDMLLLYFVYFRGILRPAISLKNLMMLKEVIIS
Sbjct: 1   MNGSVVLRRSATSFLLASERYIYSFPSLDMLLLYFVYFRGILRPAISLKNLMMLKEVIIS 60

Query: 61  LMLRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNINSQRYLEALFIACQNLTRETSPSS 120
           LMLRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNINSQRYLEALFIACQNLTRETSPSS
Sbjct: 61  LMLRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNINSQRYLEALFIACQNLTRETSPSS 120

Query: 121 LGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIMLPELTGKLPLRTCRGVVAHFQLPD 180
           LGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIMLPELTGKLPLRTCRGVVAHFQLPD
Sbjct: 121 LGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIMLPELTGKLPLRTCRGVVAHFQLPD 180

Query: 181 DISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWKSTNSSPEVSEEEGSRAVAELLPKV 240
           DISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWKSTNSSPEVSEEEGSRAVAELLPKV
Sbjct: 181 DISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWKSTNSSPEVSEEEGSRAVAELLPKV 240

Query: 241 SAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGCIDEIVRPNSSCKYWLFGGLGSRGL 300
           SAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGCIDEIVRPNSSCKYWLFGGLGSRGL
Sbjct: 241 SAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGCIDEIVRPNSSCKYWLFGGLGSRGL 300

Query: 301 LYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           LYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS
Sbjct: 301 LYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 335

[Close]

BLAST of Carg09974 vs. NCBI nr
Match: KAG6589795.1 (Dicer-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 565.1 bits (1455), Expect = 4.1e-157
Identity = 283/299 (94.65%), Postives = 289/299 (96.66%), Query Frame = 0


Query: 39   RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
            RGILRPAISLKNLMMLKE   + +   R++++  DAAQNLVPKLNIPLNTAFYMPQAVNI
Sbjct: 2241 RGILRPAISLKNLMMLKENAQNGLDGCRIETIDGDAAQNLVPKLNIPLNTAFYMPQAVNI 2300

Query: 99   NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
            NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM
Sbjct: 2301 NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 2360

Query: 159  LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
            LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 2361 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 2420

Query: 219  STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
            STNSSP VSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 2421 STNSSPGVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 2480

Query: 279  IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
            IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS
Sbjct: 2481 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 2539

[Close]

BLAST of Carg09974 vs. NCBI nr
Match: XP_022921836.1 (uncharacterized protein LOC111429976 [Cucurbita moschata])

HSP 1 Score: 563.1 bits (1450), Expect = 1.6e-156
Identity = 281/299 (93.98%), Postives = 289/299 (96.66%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAAQNLVPKLNIPLNTAFYMPQAVNI
Sbjct: 127 RGILRPAISLKNLMMLKENAQNGLDSCRIETIDGDAAQNLVPKLNIPLNTAFYMPQAVNI 186

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           N+QRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSI ELGELEGTYDAVIVCLGAKMIM
Sbjct: 187 NAQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSIKELGELEGTYDAVIVCLGAKMIM 246

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 247 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 306

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 307 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 366

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS
Sbjct: 367 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 425

[Close]

BLAST of Carg09974 vs. NCBI nr
Match: XP_023516816.1 (uncharacterized protein LOC111780607 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 280/299 (93.65%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAAQNLVPKLNIPLNTAFYMPQAVNI
Sbjct: 131 RGILRPAISLKNLMMLKENAQNGLDSCRIETIDGDAAQNLVPKLNIPLNTAFYMPQAVNI 190

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM
Sbjct: 191 NSQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 250

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LP+LTGKLPLRTCRGVVAHFQL DDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 251 LPQLTGKLPLRTCRGVVAHFQLHDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 310

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 311 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 370

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSC EELIPSELISWKKVMNS
Sbjct: 371 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCNEELIPSELISWKKVMNS 429

[Close]

BLAST of Carg09974 vs. NCBI nr
Match: XP_022987510.1 (uncharacterized protein LOC111485051 isoform X2 [Cucurbita maxima])

HSP 1 Score: 558.5 bits (1438), Expect = 3.8e-155
Identity = 279/299 (93.31%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAA+NLVPKL IPLNTAFYMPQAVNI
Sbjct: 15  RGILRPAISLKNLMMLKENARNGLDSCRIETIDEDAARNLVPKLYIPLNTAFYMPQAVNI 74

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSIN+LGELEGTYDAVIVCLGAKMIM
Sbjct: 75  NSQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSINDLGELEGTYDAVIVCLGAKMIM 134

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 135 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 194

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 195 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 254

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSC EELIPSELISWKKVMNS
Sbjct: 255 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCNEELIPSELISWKKVMNS 313

[Close]

BLAST of Carg09974 vs. ExPASy TrEMBL
Match: A0A6J1E2H0 (uncharacterized protein LOC111429976 OS=Cucurbita moschata OX=3662 GN=LOC111429976 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.5e-157
Identity = 281/299 (93.98%), Postives = 289/299 (96.66%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAAQNLVPKLNIPLNTAFYMPQAVNI
Sbjct: 127 RGILRPAISLKNLMMLKENAQNGLDSCRIETIDGDAAQNLVPKLNIPLNTAFYMPQAVNI 186

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           N+QRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSI ELGELEGTYDAVIVCLGAKMIM
Sbjct: 187 NAQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSIKELGELEGTYDAVIVCLGAKMIM 246

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 247 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 306

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 307 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 366

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS
Sbjct: 367 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 425

[Close]

BLAST of Carg09974 vs. ExPASy TrEMBL
Match: A0A6J1JJ28 (uncharacterized protein LOC111485051 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485051 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.9e-155
Identity = 279/299 (93.31%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAA+NLVPKL IPLNTAFYMPQAVNI
Sbjct: 131 RGILRPAISLKNLMMLKENARNGLDSCRIETIDEDAARNLVPKLYIPLNTAFYMPQAVNI 190

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSIN+LGELEGTYDAVIVCLGAKMIM
Sbjct: 191 NSQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSINDLGELEGTYDAVIVCLGAKMIM 250

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 251 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 310

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 311 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 370

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSC EELIPSELISWKKVMNS
Sbjct: 371 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCNEELIPSELISWKKVMNS 429

[Close]

BLAST of Carg09974 vs. ExPASy TrEMBL
Match: A0A6J1JH27 (uncharacterized protein LOC111485051 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485051 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.9e-155
Identity = 279/299 (93.31%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLMMLKE   + +   R++++  DAA+NLVPKL IPLNTAFYMPQAVNI
Sbjct: 15  RGILRPAISLKNLMMLKENARNGLDSCRIETIDEDAARNLVPKLYIPLNTAFYMPQAVNI 74

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIACQNLT+ETSPSSLGHKELFLQKKSIN+LGELEGTYDAVIVCLGAKMIM
Sbjct: 75  NSQRYLEALFIACQNLTKETSPSSLGHKELFLQKKSINDLGELEGTYDAVIVCLGAKMIM 134

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK
Sbjct: 135 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 194

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC
Sbjct: 195 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 254

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSC EELIPSELISWKKVMNS
Sbjct: 255 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCNEELIPSELISWKKVMNS 313

[Close]

BLAST of Carg09974 vs. ExPASy TrEMBL
Match: A0A5D3BXR2 (Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00600 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.9e-140
Identity = 251/299 (83.95%), Postives = 274/299 (91.64%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLM+LKE   + +   R++++   +A++LVP+L +PLNTAFYMPQAVNI
Sbjct: 131 RGILRPAISLKNLMVLKENAQNGLDSCRIETIDEVSARDLVPELCVPLNTAFYMPQAVNI 190

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIAC+NLT+ETS S  GHKEL+LQKKSIN+L ELEGTYDAVIVCLGAKM+M
Sbjct: 191 NSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAVIVCLGAKMVM 250

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQL DDISNAYP LGPSILSDAWLAI+ PRSLYMGSTWEWK
Sbjct: 251 LPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRSLYMGSTWEWK 310

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEE SRAVAELLPKVSAIYPSIKEWSF RAR GLRAMPPL PHGSLPLMGC
Sbjct: 311 STNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLNPHGSLPLMGC 370

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEI+R NSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C +ELIPSEL SWKK+MNS
Sbjct: 371 IDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSELTSWKKIMNS 429

[Close]

BLAST of Carg09974 vs. ExPASy TrEMBL
Match: A0A5A7U967 (Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00030 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.9e-140
Identity = 251/299 (83.95%), Postives = 274/299 (91.64%), Query Frame = 0


Query: 39  RGILRPAISLKNLMMLKEVIISLM--LRMDSMAADAAQNLVPKLNIPLNTAFYMPQAVNI 98
           RGILRPAISLKNLM+LKE   + +   R++++   +A++LVP+L +PLNTAFYMPQAVNI
Sbjct: 131 RGILRPAISLKNLMVLKENARNGLDSCRIETIDEVSARDLVPELCVPLNTAFYMPQAVNI 190

Query: 99  NSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLGAKMIM 158
           NSQRYLEALFIAC+NLT+ETS S  GHKEL+LQKKSIN+L ELEGTYDAVIVCLGAKM+M
Sbjct: 191 NSQRYLEALFIACENLTKETSTSGFGHKELYLQKKSINKLRELEGTYDAVIVCLGAKMVM 250

Query: 159 LPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGSTWEWK 218
           LPELTGKLPLRTCRGVVAHFQL DDISNAYP LGPSILSDAWLAI+ PRSLYMGSTWEWK
Sbjct: 251 LPELTGKLPLRTCRGVVAHFQLHDDISNAYPGLGPSILSDAWLAIRGPRSLYMGSTWEWK 310

Query: 219 STNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSLPLMGC 278
           STNSSPEVSEEE SRAVAELLPKVSAIYPSIKEWSF RAR GLRAMPPL PHGSLPLMGC
Sbjct: 311 STNSSPEVSEEESSRAVAELLPKVSAIYPSIKEWSFIRARGGLRAMPPLNPHGSLPLMGC 370

Query: 279 IDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKKVMNS 336
           IDEI+R NSSCKYW+FGGLGSRGLLYHGWLGKLTAQAVL+C +ELIPSEL SWKK+MNS
Sbjct: 371 IDEIIRHNSSCKYWVFGGLGSRGLLYHGWLGKLTAQAVLTCNDELIPSELTSWKKIMNS 429

[Close]

BLAST of Carg09974 vs. TAIR 10
Match: AT2G22650.1 (FAD-dependent oxidoreductase family protein )

HSP 1 Score: 362.1 bits (928), Expect = 4.9e-100
Identity = 173/300 (57.67%), Postives = 225/300 (75.00%), Query Frame = 0


Query: 34  YFVYFRGILRPAISLKNLMMLKEVIISLMLR--MDSMAADAAQNLVPKLNIPLNTAFYMP 93
           + V  RGILRPA + K L ++ +   + +    ++++  D AQNLVP L +PLN AFY P
Sbjct: 122 FMVRRRGILRPATNAKTLDLMSDNARNCLASCVVETIDKDGAQNLVPNLYLPLNCAFYFP 181

Query: 94  QAVNINSQRYLEALFIACQNLTRETSPSSLGHKELFLQKKSINELGELEGTYDAVIVCLG 153
            A+N+N  RYL+ALF+ACQ+    ++ +SLG K + L  KSI ++ ELEG YDAV++CLG
Sbjct: 182 GAMNVNPHRYLQALFLACQS----SAKTSLGRKNITLVNKSITDVLELEGGYDAVVICLG 241

Query: 154 AKMIMLPELTGKLPLRTCRGVVAHFQLPDDISNAYPELGPSILSDAWLAIQSPRSLYMGS 213
           +K+  LP LTGKLPLRTCRGV+ H QL +    +YP+ GPSILSDAWLA+Q PR+L+MGS
Sbjct: 242 SKVDFLPGLTGKLPLRTCRGVITHMQLHESARGSYPDDGPSILSDAWLAVQGPRNLHMGS 301

Query: 214 TWEWKSTNSSPEVSEEEGSRAVAELLPKVSAIYPSIKEWSFRRARAGLRAMPPLTPHGSL 273
           TWEW+S N S +V  EE SRA+ ELLPK SA+YP I++W F  ARAGLRAMPP+T HGSL
Sbjct: 302 TWEWQSRNYSSDVPAEEASRALNELLPKASAVYPDIEKWEFAGARAGLRAMPPVTSHGSL 361

Query: 274 PLMGCIDEIVRPNSSCKYWLFGGLGSRGLLYHGWLGKLTAQAVLSCKEELIPSELISWKK 332
           PL+GC+++++     C +W+F GLGSRGLLYH WLGKL ++AVLSC EEL+PSEL SWKK
Sbjct: 362 PLLGCVNQLIGAGQFCNFWVFSGLGSRGLLYHAWLGKLMSKAVLSCNEELLPSELTSWKK 417

The following BLAST results are available for this feature:

BLAST of Carg09974 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023465.1	1.0e-187	100.00	hypothetical protein SDJN02_14490 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6589795.1	4.1e-157	94.65	Dicer-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022921836.1	1.6e-156	93.98	uncharacterized protein LOC111429976 [Cucurbita moschata]	[more]
XP_023516816.1	1.3e-155	93.65	uncharacterized protein LOC111780607 [Cucurbita pepo subsp. pepo]	[more]
XP_022987510.1	3.8e-155	93.31	uncharacterized protein LOC111485051 isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg09974 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg09974 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2H0	7.5e-157	93.98	uncharacterized protein LOC111429976 OS=Cucurbita moschata OX=3662 GN=LOC1114299...	[more]
A0A6J1JJ28	1.9e-155	93.31	uncharacterized protein LOC111485051 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1JH27	1.9e-155	93.31	uncharacterized protein LOC111485051 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A5D3BXR2	2.9e-140	83.95	Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa O...	[more]
A0A5A7U967	2.9e-140	83.95	Putative thiamine biosynthesis oxidoreductase ThiO OS=Cucumis melo var. makuwa O...	[more]

BLAST of Carg09974 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22650.1	4.9e-100	57.67	FAD-dependent oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.9.10		coord: 63..271 e-value: 4.4E-9 score: 37.8
None	No IPR available	PANTHER	PTHR13847:SF261	FAD-DEPENDENT OXIDOREDUCTASE FAMILY PROTEIN	coord: 39..320
None	No IPR available	PANTHER	PTHR13847	SARCOSINE DEHYDROGENASE-RELATED	coord: 39..320
None	No IPR available	SUPERFAMILY	51971	Nucleotide-binding domain	coord: 39..317
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 97..316 e-value: 4.4E-9 score: 37.8
IPR006076	FAD dependent oxidoreductase	PFAM	PF01266	DAO	coord: 71..312 e-value: 6.6E-17 score: 62.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09974-RA	Carg09974-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006270	DNA replication initiation
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042555	MCM complex
cellular_component	GO:0000347	THO complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003678	DNA helicase activity
molecular_function	GO:0003688	DNA replication origin binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0004672	protein kinase activity