Carg09724 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09724
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	50S ribosomal protein L12, chloroplastic-like
Location	Carg_Chr14: 3291925 .. 3292497 (-)
RNA-Seq Expression	Carg09724
Synteny	Carg09724

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCCGCTCTCTCCACCGCTTCACTCCGGTTACCTTCTTGTTCTTCATCCTCTTATCCATCAAATTTCTCCCATTCCCTCAGATCCACTTCAATCCAATTTCCCTTCTCTTTCTCCTCCACCAATCTCTCTCTTCGTTTTGTTCATTGCCGCCCACTCTCTGCCGCCGCTGTGCCGGAGAAAGTTACAGAAATCGGCGATACCATCTCCAAGTTGACTCTCGAAGAGGCTCGTACTCTAGTTGATTACCTCCAAGAGAAACTCGGTGTTTCCGCTGCCGCTTTCGCGCCTGCCGCCGTCGCCGTTGCTCCTGGCGCGGCTGCCGGAGGTGAAGATGCGGCTTCCGTTGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGAAGTCCCTAGTAATGCGAGGATTTCGGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAAGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGAATATCGAAGGAAGAGGCGGAGGATGCGAAGAAGCAGCTTGAAGAGGCTGGCGCAAAGATTGCTATCGTTTGA

mRNA sequence

ATGTCTTCCGCTCTCTCCACCGCTTCACTCCGGTTACCTTCTTGTTCTTCATCCTCTTATCCATCAAATTTCTCCCATTCCCTCAGATCCACTTCAATCCAATTTCCCTTCTCTTTCTCCTCCACCAATCTCTCTCTTCGTTTTGTTCATTGCCGCCCACTCTCTGCCGCCGCTGTGCCGGAGAAAGTTACAGAAATCGGCGATACCATCTCCAAGTTGACTCTCGAAGAGGCTCGTACTCTAGTTGATTACCTCCAAGAGAAACTCGGTGTTTCCGCTGCCGCTTTCGCGCCTGCCGCCGTCGCCGTTGCTCCTGGCGCGGCTGCCGGAGGTGAAGATGCGGCTTCCGTTGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGAAGTCCCTAGTAATGCGAGGATTTCGGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAAGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGAATATCGAAGGAAGAGGCGGAGGATGCGAAGAAGCAGCTTGAAGAGGCTGGCGCAAAGATTGCTATCGTTTGA

Coding sequence (CDS)

ATGTCTTCCGCTCTCTCCACCGCTTCACTCCGGTTACCTTCTTGTTCTTCATCCTCTTATCCATCAAATTTCTCCCATTCCCTCAGATCCACTTCAATCCAATTTCCCTTCTCTTTCTCCTCCACCAATCTCTCTCTTCGTTTTGTTCATTGCCGCCCACTCTCTGCCGCCGCTGTGCCGGAGAAAGTTACAGAAATCGGCGATACCATCTCCAAGTTGACTCTCGAAGAGGCTCGTACTCTAGTTGATTACCTCCAAGAGAAACTCGGTGTTTCCGCTGCCGCTTTCGCGCCTGCCGCCGTCGCCGTTGCTCCTGGCGCGGCTGCCGGAGGTGAAGATGCGGCTTCCGTTGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGAAGTCCCTAGTAATGCGAGGATTTCGGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAAGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGAATATCGAAGGAAGAGGCGGAGGATGCGAAGAAGCAGCTTGAAGAGGCTGGCGCAAAGATTGCTATCGTTTGA

Protein sequence

MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLEEAGAKIAIV

Homology

[Close]

BLAST of Carg09724 vs. NCBI nr
Match: KAG7017767.1 (50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK
Sbjct: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. NCBI nr
Match: KAG6581037.1 (50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.4 bits (851), Expect = 2.5e-87
Identity = 189/190 (99.47%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAA+VEEK
Sbjct: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. NCBI nr
Match: XP_022983181.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 328.2 bits (840), Expect = 4.8e-86
Identity = 186/190 (97.89%), Postives = 189/190 (99.47%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGD ISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAA+GG+DAA+VEEK
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAASGGDDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. NCBI nr
Match: XP_023526119.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.2 bits (835), Expect = 1.8e-85
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGD ISKLTLEEARTLVDYLQEKLGVSAAAFAPAA  VAPGAAAGG+DAA+VEEK
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAAVVAPGAAAGGDDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. NCBI nr
Match: XP_022934872.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 326.2 bits (835), Expect = 1.8e-85
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLR PSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRFPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGD ISKLTLEEARTLVDYLQEKLGVSAAAFAPAAV VAPGAAAGG+DAA+VEEK
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVVVAPGAAAGGDDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. ExPASy Swiss-Prot
Match: P24929 (50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-48
Identity = 123/189 (65.08%), Postives = 148/189 (78.31%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           M+S LST +LR PS S++S  ++ S      +++FP      +   R    RPL+A   P
Sbjct: 1   MASTLSTITLRSPSPSTAS-STHASIPFPKKALEFPIRTPKLH-HRRATFLRPLAAVEAP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKV ++GD IS LTL +A+ LV+YLQ+KLGV+AA+FAPAAVA APGAAA  E  A VEEK
Sbjct: 61  EKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVAAAPGAAA--EAPAVVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVI+EVPSNARI+ IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EAEDAKKQL
Sbjct: 121 TEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEAEDAKKQL 180

Query: 181 EEAGAKIAI 190
           EEAGAK++I
Sbjct: 181 EEAGAKVSI 185

[Close]

BLAST of Carg09724 vs. ExPASy Swiss-Prot
Match: P36688 (50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.0e-48
Identity = 121/189 (64.02%), Postives = 147/189 (77.78%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           M+S LST +LR PS S+++  ++ S      +++FP        + R    RPL+A   P
Sbjct: 1   MASTLSTITLRSPSPSTAT-STHASIPFPKKTLEFPIRTPKLQ-NRRATFLRPLAAVEAP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKV ++GD IS LTL +A+ LV+YLQ+KLGV+AA+FAPAAV  APGAAA  E  A VEEK
Sbjct: 61  EKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVVAAPGAAA--EAPAVVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVI+EVPSNARI+ IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EAEDAKKQL
Sbjct: 121 TEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEAEDAKKQL 180

Query: 181 EEAGAKIAI 190
           EEAGAK++I
Sbjct: 181 EEAGAKVSI 185

[Close]

BLAST of Carg09724 vs. ExPASy Swiss-Prot
Match: P02398 (50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-47
Identity = 117/193 (60.62%), Postives = 148/193 (76.68%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSL---RFVHCRPLSAA 60
           M++  + A+L LPS   +S+P++ +       +QFPF  ++  +SL   R    RP++A 
Sbjct: 1   MAATTTMATLNLPSL--TSHPNSSTFPKHPQPLQFPFRTTTNPISLSSTRTTRLRPIAAV 60

Query: 61  AVPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASV 120
             PEK+ ++G  +S LTLEEAR LVD+LQ+KLGVSAA+FAPAA   APGA A  + A +V
Sbjct: 61  EAPEKIEQLGTQLSGLTLEEARVLVDWLQDKLGVSAASFAPAAAVAAPGAPA--DAAPAV 120

Query: 121 EEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDV I+EVPSNARISVIKAVRALTSL LKEAKELIEGLPKK KEG+SK++AEDAK
Sbjct: 121 EEKTEFDVSIDEVPSNARISVIKAVRALTSLGLKEAKELIEGLPKKLKEGVSKDDAEDAK 180

Query: 181 KQLEEAGAKIAIV 191
           KQLE+AGAK++IV
Sbjct: 181 KQLEDAGAKVSIV 189

[Close]

BLAST of Carg09724 vs. ExPASy Swiss-Prot
Match: P36212 (50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12C PE=2 SV=2)

HSP 1 Score: 183.7 bits (465), Expect = 1.9e-45
Identity = 120/188 (63.83%), Postives = 143/188 (76.06%), Query Frame = 0


Query: 4   ALSTASLRLPSCSSSSYPSNFSHSLRS--TSIQFPFSFSSTNLSLRFVHCRPLSAAAVPE 63
           A +T S+     SSS   S  +H   S  T+I+FPF  SS++ S R ++ RP+SA   PE
Sbjct: 2   ASTTLSIATTIRSSSPLTSASTHHFLSKPTAIEFPFRLSSSS-SHRAINLRPISAVEAPE 61

Query: 64  KVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEKT 123
           K+ +IG  IS LTLEEAR LVDYLQ+K GVS  + APAA AVA  A A G  AA VEE+T
Sbjct: 62  KIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVA--APADGGAAAVVEEQT 121

Query: 124 EFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLE 183
           EFDVVI EVPS++RI+VIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK LE
Sbjct: 122 EFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKKTLE 181

Query: 184 EAGAKIAI 190
           EAGAK++I
Sbjct: 182 EAGAKVSI 186

[Close]

BLAST of Carg09724 vs. ExPASy Swiss-Prot
Match: P36210 (50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12A PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.5e-45
Identity = 122/194 (62.89%), Postives = 148/194 (76.29%), Query Frame = 0


Query: 2   SSALSTASLRLPSCSSSSYP--SNFSH-SLRSTSIQFPFSF---SSTNLSLRFVHCRPLS 61
           S+ LS A+    +  SSSYP  ++ +H   R+T+I+FP  F   SS+ L+ R  H RP++
Sbjct: 3   STTLSIAT----TIRSSSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHLRPIA 62

Query: 62  AAAVPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAA 121
           A   PEK+ +IG  IS LTLEEAR LVDYLQ+K GVS  + APAA AVA  A A G  AA
Sbjct: 63  AVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVA--APADGGAAA 122

Query: 122 SVEEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAED 181
            VEE+TEFDVVI EVPS++RI+VIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+
Sbjct: 123 VVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEE 182

Query: 182 AKKQLEEAGAKIAI 190
           AKK LEEAGAK++I
Sbjct: 183 AKKTLEEAGAKVSI 190

[Close]

BLAST of Carg09724 vs. ExPASy TrEMBL
Match: A0A6J1J1D9 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481837 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.3e-86
Identity = 186/190 (97.89%), Postives = 189/190 (99.47%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGD ISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAA+GG+DAA+VEEK
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAASGGDDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. ExPASy TrEMBL
Match: A0A6J1F403 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441912 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 8.8e-86
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSSALSTASLR PSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP
Sbjct: 1   MSSALSTASLRFPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEK 120
           EKVTEIGD ISKLTLEEARTLVDYLQEKLGVSAAAFAPAAV VAPGAAAGG+DAA+VEEK
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVVVAPGAAAGGDDAAAVEEK 120

Query: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180
           TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL
Sbjct: 121 TEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQL 180

Query: 181 EEAGAKIAIV 191
           EEAGAKIAIV
Sbjct: 181 EEAGAKIAIV 190

[Close]

BLAST of Carg09724 vs. ExPASy TrEMBL
Match: A0A5A7UJJ4 (50S ribosomal protein L12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1545G00330 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.7e-74
Identity = 169/193 (87.56%), Postives = 180/193 (93.26%), Query Frame = 0


Query: 1   MSSALSTASLR--LPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAA 60
           MSS LSTASLR   PS SSSSYPSNFSHSL+ T ++FPFSF+ TNLSLR +H RP+SA A
Sbjct: 1   MSSTLSTASLRSPSPSSSSSSYPSNFSHSLKPTPLRFPFSFAPTNLSLRLIHHRPISAVA 60

Query: 61  VPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGED-AASV 120
           VPEKV+EIGD ISKLTLEEARTLVDYLQEKLGVSAA+FAPAAVAVAPGAAAGG+D AA+V
Sbjct: 61  VPEKVSEIGDVISKLTLEEARTLVDYLQEKLGVSAASFAPAAVAVAPGAAAGGDDAAAAV 120

Query: 121 EEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDVVIEEVPSNARI+VIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK
Sbjct: 121 EEKTEFDVVIEEVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180

Query: 181 KQLEEAGAKIAIV 191
           KQLEEAGAKIAIV
Sbjct: 181 KQLEEAGAKIAIV 193

[Close]

BLAST of Carg09724 vs. ExPASy TrEMBL
Match: A0A1S3B8B3 (50S ribosomal protein L12, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103487096 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.7e-74
Identity = 169/193 (87.56%), Postives = 180/193 (93.26%), Query Frame = 0


Query: 1   MSSALSTASLR--LPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAA 60
           MSS LSTASLR   PS SSSSYPSNFSHSL+ T ++FPFSF+ TNLSLR +H RP+SA A
Sbjct: 1   MSSTLSTASLRSPSPSSSSSSYPSNFSHSLKPTPLRFPFSFAPTNLSLRLIHHRPISAVA 60

Query: 61  VPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGED-AASV 120
           VPEKV+EIGD ISKLTLEEARTLVDYLQEKLGVSAA+FAPAAVAVAPGAAAGG+D AA+V
Sbjct: 61  VPEKVSEIGDVISKLTLEEARTLVDYLQEKLGVSAASFAPAAVAVAPGAAAGGDDAAAAV 120

Query: 121 EEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDVVIEEVPSNARI+VIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK
Sbjct: 121 EEKTEFDVVIEEVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180

Query: 181 KQLEEAGAKIAIV 191
           KQLEEAGAKIAIV
Sbjct: 181 KQLEEAGAKIAIV 193

[Close]

BLAST of Carg09724 vs. ExPASy TrEMBL
Match: A0A0A0LHZ4 (Ribosomal protein L12-C OS=Cucumis sativus OX=3659 GN=Csa_3G837570 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.8e-73
Identity = 163/191 (85.34%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60
           MSS LSTASLR PS SSSSYPSNFSHSL+ T ++FPFSF+ TNLSLR +H RP+SA AVP
Sbjct: 1   MSSTLSTASLRSPSSSSSSYPSNFSHSLKPTPLRFPFSFAPTNLSLRLIHHRPISAVAVP 60

Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGED-AASVEE 120
           EKV+EIGD ISKLTLEEARTLVDYLQEKLGVSAA+FAPA   VAPG AAGG+D AA+VEE
Sbjct: 61  EKVSEIGDVISKLTLEEARTLVDYLQEKLGVSAASFAPAPATVAPGTAAGGDDAAAAVEE 120

Query: 121 KTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEEVPSNARI+VIK+VRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEEVPSNARIAVIKSVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 191
           LEEAGAKI+IV
Sbjct: 181 LEEAGAKISIV 191

[Close]

BLAST of Carg09724 vs. TAIR 10
Match: AT3G27850.1 (ribosomal protein L12-C )

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-46
Identity = 120/188 (63.83%), Postives = 143/188 (76.06%), Query Frame = 0


Query: 4   ALSTASLRLPSCSSSSYPSNFSHSLRS--TSIQFPFSFSSTNLSLRFVHCRPLSAAAVPE 63
           A +T S+     SSS   S  +H   S  T+I+FPF  SS++ S R ++ RP+SA   PE
Sbjct: 2   ASTTLSIATTIRSSSPLTSASTHHFLSKPTAIEFPFRLSSSS-SHRAINLRPISAVEAPE 61

Query: 64  KVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVEEKT 123
           K+ +IG  IS LTLEEAR LVDYLQ+K GVS  + APAA AVA  A A G  AA VEE+T
Sbjct: 62  KIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVA--APADGGAAAVVEEQT 121

Query: 124 EFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLE 183
           EFDVVI EVPS++RI+VIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK LE
Sbjct: 122 EFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKKTLE 181

Query: 184 EAGAKIAI 190
           EAGAK++I
Sbjct: 182 EAGAKVSI 186

[Close]

BLAST of Carg09724 vs. TAIR 10
Match: AT3G27830.1 (ribosomal protein L12-A )

HSP 1 Score: 183.3 bits (464), Expect = 1.8e-46
Identity = 122/194 (62.89%), Postives = 148/194 (76.29%), Query Frame = 0


Query: 2   SSALSTASLRLPSCSSSSYP--SNFSH-SLRSTSIQFPFSF---SSTNLSLRFVHCRPLS 61
           S+ LS A+    +  SSSYP  ++ +H   R+T+I+FP  F   SS+ L+ R  H RP++
Sbjct: 3   STTLSIAT----TIRSSSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHLRPIA 62

Query: 62  AAAVPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAA 121
           A   PEK+ +IG  IS LTLEEAR LVDYLQ+K GVS  + APAA AVA  A A G  AA
Sbjct: 63  AVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVA--APADGGAAA 122

Query: 122 SVEEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAED 181
            VEE+TEFDVVI EVPS++RI+VIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+
Sbjct: 123 VVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEE 182

Query: 182 AKKQLEEAGAKIAI 190
           AKK LEEAGAK++I
Sbjct: 183 AKKTLEEAGAKVSI 190

[Close]

BLAST of Carg09724 vs. TAIR 10
Match: AT3G27840.1 (ribosomal protein L12-B )

HSP 1 Score: 159.8 bits (403), Expect = 2.1e-39
Identity = 104/192 (54.17%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 2   SSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSF---SSTNLSLRFVHCRPLSAAA 61
           ++ LS A+    S  SS   S      R  SI+FPFSF   SS+ LS R ++  P+SA  
Sbjct: 3   ATTLSIATTIRSSSFSSGLASAHHFPSRPLSIEFPFSFGVSSSSTLSHRAIYLHPISAVK 62

Query: 62  VPEKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAGGEDAASVE 121
            P+K+ +IG  IS LTLEE+R LVDY+Q+K GVS    APAA A+ P    GG   ASVE
Sbjct: 63  TPKKIKKIGSEISSLTLEESRILVDYVQDKFGVSILFSAPAAAALPPPLDNGGA-TASVE 122

Query: 122 EKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 181
            +T FDVVI +VP   RI+VI A+RA+TSL+L E+KELIEG PKKFKEG++K+EAE+ K 
Sbjct: 123 RQTTFDVVINDVPRGNRIAVITAIRAMTSLSLSESKELIEGFPKKFKEGVTKDEAEEDKT 182

Query: 182 QLEEAGAKIAIV 191
           QLEEAGAK++IV
Sbjct: 183 QLEEAGAKVSIV 193

[Close]

BLAST of Carg09724 vs. TAIR 10
Match: AT4G36420.1 (Ribosomal protein L12 family protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.6e-16
Identity = 68/176 (38.64%), Postives = 103/176 (58.52%), Query Frame = 0


Query: 22  SNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAV-PEKVTEIGDTISKLTLEEART 81
           S+FS  L+ST  +      S+++ L  +  R  S+ A   E V++I + +S LTL E   
Sbjct: 9   SHFSRVLKSTETR------SSSVQLFTIQSRSYSSPATQSENVSKIVNELSNLTLLETMD 68

Query: 82  LVDYLQEKLGVS----AAAFAPAAVAVAPGA--AAGGEDAASVEE-KTEFDVVIEEVPSN 141
           L + L++KL +S     AA  P       GA  +AGGE     +E KT FDV+++   + 
Sbjct: 69  LTEILRQKLNISELPVMAAMMPGMSLPGSGASKSAGGEGKEKKKEAKTAFDVMLQAYDAV 128

Query: 142 ARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLEEAGAKIAI 190
           ++I VIK VR +T+L LKEAK+L+E  P   K+G+SKEEAE   ++L+  GAK+A+
Sbjct: 129 SKIKVIKEVRTITALGLKEAKDLVEKAPTLLKKGVSKEEAEKIIEKLKAVGAKVAM 178

[Close]

BLAST of Carg09724 vs. TAIR 10
Match: AT1G70190.1 (Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like )

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-10
Identity = 48/142 (33.80%), Postives = 73/142 (51.41%), Query Frame = 0


Query: 61  EKVTEIGDTISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAAAG---------- 120
           ++V  + D IS LTL E   L   + +K G++     P    + PGA  G          
Sbjct: 69  DRVFRLVDEISSLTLLEISELGSIIMKKKGMTE---LPTVAVMKPGAGGGVGVGGGGGMV 128

Query: 121 ---GEDAASVEEKTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEG 180
              G    +  EKT F++ +E   ++A+I +IK +R+ T L LKEAK L+E  P   K G
Sbjct: 129 SQSGVSEEAKVEKTVFEIKLESFEASAKIKIIKELRSFTDLGLKEAKALVEKTPAILKAG 188

Query: 181 ISKEEAEDAKKQLEEAGAKIAI 190
           +SKEE E   ++L+  GAK+ +
Sbjct: 189 LSKEEGEKILEKLKALGAKVVL 207

The following BLAST results are available for this feature:

BLAST of Carg09724 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017767.1	1.1e-87	100.00	50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
KAG6581037.1	2.5e-87	99.47	50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022983181.1	4.8e-86	97.89	50S ribosomal protein L12, chloroplastic-like [Cucurbita maxima]	[more]
XP_023526119.1	1.8e-85	97.37	50S ribosomal protein L12, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934872.1	1.8e-85	97.37	50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Carg09724 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24929	1.4e-48	65.08	50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 P...	[more]
P36688	7.0e-48	64.02	50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV...	[more]
P02398	1.2e-47	60.62	50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 P...	[more]
P36212	1.9e-45	63.83	50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]
P36210	2.5e-45	62.89	50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]

BLAST of Carg09724 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J1D9	2.3e-86	97.89	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1F403	8.8e-86	97.37	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5A7UJJ4	2.7e-74	87.56	50S ribosomal protein L12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3B8B3	2.7e-74	87.56	50S ribosomal protein L12, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A0A0LHZ4	3.8e-73	85.34	Ribosomal protein L12-C OS=Cucumis sativus OX=3659 GN=Csa_3G837570 PE=3 SV=1	[more]

BLAST of Carg09724 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27850.1	1.4e-46	63.83	ribosomal protein L12-C	[more]
AT3G27830.1	1.8e-46	62.89	ribosomal protein L12-A	[more]
AT3G27840.1	2.1e-39	54.17	ribosomal protein L12-B	[more]
AT4G36420.1	5.6e-16	38.64	Ribosomal protein L12 family protein	[more]
AT1G70190.1	1.6e-10	33.80	Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/a...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 123..189 e-value: 3.9E-24 score: 84.8
IPR008932	Ribosomal protein L7/L12, oligomerisation	PFAM	PF16320	Ribosomal_L12_N	coord: 62..110 e-value: 3.8E-15 score: 55.3
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	GENE3D	1.20.5.710	Single helix bin	coord: 56..118 e-value: 8.4E-14 score: 53.3
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	SUPERFAMILY	48300	Ribosomal protein L7/12, oligomerisation (N-terminal) domain	coord: 62..125
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 119..189 e-value: 1.3E-25 score: 91.3
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	SUPERFAMILY	54736	ClpS-like	coord: 118..189
IPR000206	Ribosomal protein L7/L12	TIGRFAM	TIGR00855	TIGR00855	coord: 64..189 e-value: 2.9E-33 score: 112.9
IPR000206	Ribosomal protein L7/L12	PANTHER	PTHR45987	39S RIBOSOMAL PROTEIN L12	coord: 1..189
IPR000206	Ribosomal protein L7/L12	HAMAP	MF_00368	Ribosomal_L7_L12	coord: 58..190 score: 23.019552
IPR000206	Ribosomal protein L7/L12	CDD	cd00387	Ribosomal_L7_L12	coord: 61..189 e-value: 1.4354E-41 score: 134.59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR45987:SF16	50S RIBOSOMAL PROTEIN L12-1, CHLOROPLASTIC-RELATED	coord: 1..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09724-RA	Carg09724-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome