Carg09601 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09601
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	calcineurin subunit B
Location	Carg_Chr17: 7035107 .. 7037198 (+)
RNA-Seq Expression	Carg09601
Synteny	Carg09601

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGGTTCCATTTCTATATATGATGTGAGTAAGTAGAAGAAACCGCTCATTATCAGGTCAGCTGTGGTTTTCAGGTCTGTTCTTCTTTCCCCTTTCACCATTTTTGTCGCAGTTTTCTATCCGTTTGAAGCTTCATTGTTTGAATTTCAGTCGATCCATTAAATTTGAGGTTCTTACTTGTTCTATTCCGACTCTCTCTGTATTTGATCACTGATTTGGTTGAGCGGTTTTTATGAGGGTTTTCCGATTTCAGAAGTTCGATTCCTTAGTATCATTTAGAAATTGGGCAAGAATTCATGGGGAATACCTCGTCGATGCTCACTCAATATGATATTGAAGAAGTTCAAGAGCACTGCAATAAGCTTTGTAGGTTAATCTATACTTGTCTTGATTGGTTTATTTGTTTGTGGTTTGGTTGGCTTACTTTTTGCTGTGTTTAAGTTAGTCTCCCAACAGGAAATAGTGTCATTGTTTCAGAAATTCTGCCAGCTTGATAGAAATTCCAAGGGGTTTATTTCAGCTGATGAGTTCCTTTCAGTTCCTGAATTTGCTATGAATCCACTTCCTCAGGTATTGTTTTGAAATTGAATATAAACATTGTGTGTTTGTGTGTTGATTGTATGAGTTGCGACTTATGGCAAGCGATTTCTTTGTTTTACTCTGTTGTTATCTTCAGCGTCTGCTCAAGATGGTTGATGGTTTGAATTTTAAGGATTTTGTGGCTTTCTTGTCAGCATTCAGTGCTAAAGCTAGTTTGCACCATAAGATTGAATGTAAGTGCATGTTTTGCTACTGATTGTTCATCTTTTGCTGGTTTTGATCAGGCTTGAGGGTTTTGGTTGAAAATCAATGCTTATTTAAAACATTAGGGATCCTTAGCAATCATTCTTATGGTAGTAGTAAGTGGCACATCGTTTTTTATGAAATCCCACGTTGGTTGGAGAGGGAAAAAAAACATTTCTTAGGGTGTGAAAACCTCTCCCTAGTACACGCGTTTGACGGCTATACGTAATGAGCTAAAGTGAACAATATCTACTAGTGATGGACTTGTGCGGTTGCAAATGGTATCAGAGGCAGACATTGAACGTGTGTTAACAAGGACACTAGGCCCACAGAGGGTGGATTGTTAGATCCCACGTTCTTGCAGAAGGAACGAAGCATTCCTTATAAGGGTGTTGAAACCCCTCTCTAGCAGACGTGTTTTAAAATTGTGAGACGACAGCGATACATAACGGGCTAAAACTGACAATATCCGCTAGTGGTGGGCTTGGACTTGACTGTTACATTTTTACTCAAAAGTCATCTAACTTTTTGGTTGTCTTCCTCAATTATTGGAGCAAATTTGTCACAAGAAGCCGACTTATAAGTTAGAATTCATGATATCTAGAACGGTATGGCATGAATCATCACTAAACCAAAAGATATCATGAATTATAAGTTCAGGACTAAATGGAAAAACTTTTTGTCCTGAACTGACATTAATACATGATATTGACTCTAAGAAAACTACTTTGAGATGGTTCATTTCGTATTAATAATTGCTAGTGTCTCCTTATTTCAGTTATTTTCAAGCTATATGATGTTGACTGCAACGGGAAGGTGTCTTTCAACGACATCATTGAAGTGCTTGGGGATTCGTCTAATTCATTCATCTCCGACGAGCAGAGAGAGGCATGGCGTCTTCTTTCCTTTCGTTCGATATTTCGCATCTGTGTTGCTTGTTCGATATTTCGCATCTGTGTTGCTTCTCCAATTCATCGTTATCTGAAAACGCAATGTGATTCATTTAGTATCAAATACATAGCATATAAATCCTCGGAAAGAGACACCAATCCATGCATACAGTTACTTGATGCATCTCCTTGATGATGATATGTCTGCTCGTGCATAGGCATTGAGAGTATTTACGTAGCTCGAAAGGAACATATTTAGTCCAGTCCTAACACATGAAGTGGAATAACTACATTTTCTTTGTGCTCATATTATTTCTTTCACCTTCATAAACGAACTATTATTAGTTTCTCTTTCCCATTGCAGCAAGTATTGTGCCAATTGTTACAAGAAGCAGGCTATACAAGAAACTCTCTCTTGA

mRNA sequence

GATGGTTCCATTTCTATATATGATGTGAGTAAGTAGAAGAAACCGCTCATTATCAGGTCAGCTGTGGTTTTCAGGTCTGTTCTTCTTTCCCCTTTCACCATTTTTGTCGCAGTTTTCTATCCGTTTGAAGCTTCATTGTTTGAATTTCAGTCGATCCATTAAATTTGAGGTTCTTACTTGTTCTATTCCGACTCTCTCTGTATTTGATCACTGATTTGGTTGAGCGGTTTTTATGAGGGTTTTCCGATTTCAGAAGTTCGATTCCTTAGTATCATTTAGAAATTGGGCAAGAATTCATGGGGAATACCTCGTCGATGCTCACTCAATATGATATTGAAGAAGTTCAAGAGCACTGCAATAAGCTTTTTAGTCTCCCAACAGGAAATAGTGTCATTGTTTCAGAAATTCTGCCAGCTTGATAGAAATTCCAAGGGGTTTATTTCAGCTGATGAGTTCCTTTCAGTTCCTGAATTTGCTATGAATCCACTTCCTCAGCGTCTGCTCAAGATGGTTGATGGTTTGAATTTTAAGGATTTTGTGGCTTTCTTGTCAGCATTCAGTGCTAAAGCTAGTTTGCACCATAAGATTGAATTTATTTTCAAGCTATATGATGTTGACTGCAACGGGAAGGTGTCTTTCAACGACATCATTGAAGTGCTTGGGGATTCCAAGTATTGTGCCAATTGTTACAAGAAGCAGGCTATACAAGAAACTCTCTCTTGA

Coding sequence (CDS)

ATGATATTGAAGAAGTTCAAGAGCACTGCAATAAGCTTTTTAGTCTCCCAACAGGAAATAGTGTCATTGTTTCAGAAATTCTGCCAGCTTGATAGAAATTCCAAGGGGTTTATTTCAGCTGATGAGTTCCTTTCAGTTCCTGAATTTGCTATGAATCCACTTCCTCAGCGTCTGCTCAAGATGGTTGATGGTTTGAATTTTAAGGATTTTGTGGCTTTCTTGTCAGCATTCAGTGCTAAAGCTAGTTTGCACCATAAGATTGAATTTATTTTCAAGCTATATGATGTTGACTGCAACGGGAAGGTGTCTTTCAACGACATCATTGAAGTGCTTGGGGATTCCAAGTATTGTGCCAATTGTTACAAGAAGCAGGCTATACAAGAAACTCTCTCTTGA

Protein sequence

MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANCYKKQAIQETLS

Homology

[Close]

BLAST of Carg09601 vs. NCBI nr
Match: KAG7014153.1 (Calcineurin subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 262.3 bits (669), Expect = 2.2e-66
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK
Sbjct: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANC 120
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANC
Sbjct: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANC 120

Query: 121 YKKQAIQETLS 132
           YKKQAIQETLS
Sbjct: 121 YKKQAIQETLS 131

[Close]

BLAST of Carg09601 vs. NCBI nr
Match: XP_022953695.1 (calcineurin subunit B-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 226.1 bits (575), Expect = 1.8e-55
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK
Sbjct: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 114

[Close]

BLAST of Carg09601 vs. NCBI nr
Match: XP_023548811.1 (calcineurin subunit B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.9 bits (564), Expect = 3.3e-54
Identity = 112/114 (98.25%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFL+VPEFAMNPL QRLLK
Sbjct: 9   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLAVPEFAMNPLSQRLLK 68

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 69  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 122

[Close]

BLAST of Carg09601 vs. NCBI nr
Match: XP_022991501.1 (calcineurin subunit B-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 1.4e-52
Identity = 113/132 (85.61%), Postives = 120/132 (90.91%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPL QRLLK
Sbjct: 9   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLSQRLLK 68

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGD--SKYCA 120
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCN KVSFN IIEVLGD  S + +
Sbjct: 69  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNRKVSFNHIIEVLGDLSSSFIS 128

Query: 121 NCYKKQAIQETL 131
           +  ++Q + + L
Sbjct: 129 DEEREQVLCQLL 140

[Close]

BLAST of Carg09601 vs. NCBI nr
Match: XP_038898822.1 (calcineurin subunit B-like isoform X2 [Benincasa hispida])

HSP 1 Score: 206.8 bits (525), Expect = 1.1e-49
Identity = 103/113 (91.15%), Postives = 109/113 (96.46%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAI+FLVSQQEIVSL+Q+FCQLDRN+KGFISADEFLSVPEFAMNPL QRLLK
Sbjct: 9   MILKKFKSTAINFLVSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLK 68

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGD 114
           MVDGLNFKDFVAFLSAFSAKAS+ HKIEFIFK+YD DCNGKVSFNDIIEVL D
Sbjct: 69  MVDGLNFKDFVAFLSAFSAKASVQHKIEFIFKVYDADCNGKVSFNDIIEVLRD 121

[Close]

BLAST of Carg09601 vs. ExPASy Swiss-Prot
Match: P42322 (Calcineurin subunit B OS=Naegleria gruberi OX=5762 GN=CNB1 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.8e-18
Identity = 43/101 (42.57%), Postives = 69/101 (68.32%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVD-----GLNFKDF 75
           +Q+EI  L+++F +LD++  G IS DEFL +PE A+NPL +R++ + D      +NFK+F
Sbjct: 23  TQKEIKKLYKRFKKLDKDGNGTISKDEFLMIPELAVNPLVKRVISIFDENGDGSVNFKEF 82

Query: 76  VAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
           +A LS F+A+     K+EF FK+YD+D +G +S  ++  VL
Sbjct: 83  IAALSVFNAQGDKQRKLEFAFKVYDIDGDGYISNGELFTVL 123

[Close]

BLAST of Carg09601 vs. ExPASy Swiss-Prot
Match: Q55G87 (Calcineurin subunit B type 1 OS=Dictyostelium discoideum OX=44689 GN=cnbA PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.9e-15
Identity = 36/101 (35.64%), Postives = 68/101 (67.33%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVD-----GLNFKDF 75
           S+ E+  L+++F  LD++  G ++ DEFLS+P+ A+NPL +R++++ D      + F +F
Sbjct: 23  SEAELKKLYRRFQMLDKDGSGTLTTDEFLSIPDLALNPLLERVIQIFDQNKDNEIEFFEF 82

Query: 76  VAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
           V  L+  S K +   K++F+F++YD+DC+G +S  ++ +VL
Sbjct: 83  VGTLATLSHKGTKEDKLKFLFQIYDIDCDGFISNGELFQVL 123

[Close]

BLAST of Carg09601 vs. ExPASy Swiss-Prot
Match: P0CM55 (Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNB1 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-14
Identity = 43/118 (36.44%), Postives = 70/118 (59.32%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVD-----GLNFKDF 75
           S  E++ L ++F +LD++  G I  DEFL +P+ A NPL  R++ + D      ++F++F
Sbjct: 19  SGPELMRLKKRFMKLDKDGSGSIDKDEFLQIPQIANNPLAHRMIAIFDEDGSGTVDFQEF 78

Query: 76  VAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANCYKKQAIQE 129
           V  LSAFS+K     K+ F FK+YD+D +G +S  ++  VL   +   N  K Q +Q+
Sbjct: 79  VGGLSAFSSKGGRDEKLRFAFKVYDMDRDGYISNGELYLVL--KQMVGNNLKDQQLQQ 134

[Close]

BLAST of Carg09601 vs. ExPASy Swiss-Prot
Match: P0CM54 (Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=CNB1 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-14
Identity = 43/118 (36.44%), Postives = 70/118 (59.32%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVD-----GLNFKDF 75
           S  E++ L ++F +LD++  G I  DEFL +P+ A NPL  R++ + D      ++F++F
Sbjct: 19  SGPELMRLKKRFMKLDKDGSGSIDKDEFLQIPQIANNPLAHRMIAIFDEDGSGTVDFQEF 78

Query: 76  VAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDSKYCANCYKKQAIQE 129
           V  LSAFS+K     K+ F FK+YD+D +G +S  ++  VL   +   N  K Q +Q+
Sbjct: 79  VGGLSAFSSKGGRDEKLRFAFKVYDMDRDGYISNGELYLVL--KQMVGNNLKDQQLQQ 134

[Close]

BLAST of Carg09601 vs. ExPASy Swiss-Prot
Match: P87072 (Calcineurin subunit B OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=cnb-1 PE=2 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 7.8e-14
Identity = 37/100 (37.00%), Postives = 67/100 (67.00%), Query Frame = 0


Query: 17  QQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVD-----GLNFKDFV 76
           ++E+  L ++F +LD+++ G I  +EFLS+P+ + NPL  R++ + D      ++F++FV
Sbjct: 20  REEVDRLRKRFMKLDKDNSGTIEREEFLSLPQISTNPLATRMIAIFDEDGGGDVDFQEFV 79

Query: 77  AFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
           + LSAFS+K +   K+ F FK+YD+D +G +S  ++  VL
Sbjct: 80  SGLSAFSSKGNKEQKLRFAFKVYDIDRDGYISNGELFIVL 119

[Close]

BLAST of Carg09601 vs. ExPASy TrEMBL
Match: A0A6J1GQD6 (calcineurin subunit B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456147 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 8.5e-56
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK
Sbjct: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 114

[Close]

BLAST of Carg09601 vs. ExPASy TrEMBL
Match: A0A6J1JQX3 (calcineurin subunit B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488097 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.8e-53
Identity = 113/132 (85.61%), Postives = 120/132 (90.91%), Query Frame = 0


Query: 1   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLK 60
           MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPL QRLLK
Sbjct: 9   MILKKFKSTAISFLVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLSQRLLK 68

Query: 61  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGD--SKYCA 120
           MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCN KVSFN IIEVLGD  S + +
Sbjct: 69  MVDGLNFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNRKVSFNHIIEVLGDLSSSFIS 128

Query: 121 NCYKKQAIQETL 131
           +  ++Q + + L
Sbjct: 129 DEEREQVLCQLL 140

[Close]

BLAST of Carg09601 vs. ExPASy TrEMBL
Match: A0A6J1GNZ0 (calcineurin subunit B-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111456147 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-48
Identity = 100/101 (99.01%), Postives = 100/101 (99.01%), Query Frame = 0


Query: 14  LVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 73
           L SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF
Sbjct: 23  LFSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 82

Query: 74  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 83  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 123

[Close]

BLAST of Carg09601 vs. ExPASy TrEMBL
Match: A0A6J1GP06 (calcineurin subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456147 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-48
Identity = 100/101 (99.01%), Postives = 100/101 (99.01%), Query Frame = 0


Query: 14  LVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 73
           L SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF
Sbjct: 23  LFSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 82

Query: 74  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 83  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 123

[Close]

BLAST of Carg09601 vs. ExPASy TrEMBL
Match: A0A6J1GNT2 (calcineurin subunit B-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111456147 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-48
Identity = 100/101 (99.01%), Postives = 100/101 (99.01%), Query Frame = 0


Query: 14  LVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 73
           L SQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF
Sbjct: 23  LFSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 82

Query: 74  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 115
           LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS
Sbjct: 83  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGDS 123

[Close]

BLAST of Carg09601 vs. TAIR 10
Match: AT3G18430.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 172.2 bits (435), Expect = 2.8e-43
Identity = 85/119 (71.43%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 14  LVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 73
           L  QQEI+SL+Q+FCQLDRN+KGFISADEFLSVPEFAMNPL QRLLKMVDGLNFKDFVAF
Sbjct: 23  LFEQQEILSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVDGLNFKDFVAF 82

Query: 74  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGD--SKYCANCYKKQAIQETL 131
           LSAFSAKASL  K++ IFK+YD DCNGKVSF DI+EVL D    + ++  ++Q + + L
Sbjct: 83  LSAFSAKASLRQKVQLIFKVYDSDCNGKVSFKDIMEVLRDLSGSFMSDEQREQVLSQVL 141

[Close]

BLAST of Carg09601 vs. TAIR 10
Match: AT3G18430.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 172.2 bits (435), Expect = 2.8e-43
Identity = 85/119 (71.43%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 14  LVSQQEIVSLFQKFCQLDRNSKGFISADEFLSVPEFAMNPLPQRLLKMVDGLNFKDFVAF 73
           L  QQEI+SL+Q+FCQLDRN+KGFISADEFLSVPEFAMNPL QRLLKMVDGLNFKDFVAF
Sbjct: 23  LFEQQEILSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVDGLNFKDFVAF 82

Query: 74  LSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVLGD--SKYCANCYKKQAIQETL 131
           LSAFSAKASL  K++ IFK+YD DCNGKVSF DI+EVL D    + ++  ++Q + + L
Sbjct: 83  LSAFSAKASLRQKVQLIFKVYDSDCNGKVSFKDIMEVLRDLSGSFMSDEQREQVLSQVL 141

[Close]

BLAST of Carg09601 vs. TAIR 10
Match: AT4G17615.1 (calcineurin B-like protein 1 )

HSP 1 Score: 52.4 bits (124), Expect = 3.3e-07
Identity = 34/106 (32.08%), Postives = 53/106 (50.00%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRN--SKGFISADEF---LSVPEFAMNPLPQRLLKMVD-----GL 75
           S  E+ +LF+ F  +  +    G I+ +EF   L       N    R+  M D      +
Sbjct: 28  SVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVI 87

Query: 76  NFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
           +F DFV  L+ F   ASL  KI+F F+LYD+DC G +   ++ ++L
Sbjct: 88  DFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQML 133

[Close]

BLAST of Carg09601 vs. TAIR 10
Match: AT5G47100.1 (calcineurin B-like protein 9 )

HSP 1 Score: 47.8 bits (112), Expect = 8.0e-06
Identity = 31/106 (29.25%), Postives = 52/106 (49.06%), Query Frame = 0


Query: 16  SQQEIVSLFQKFCQLDRN--SKGFISADEF---LSVPEFAMNPLPQRLLKMVD-----GL 75
           S  E+ +L++ F  +  +    G I+ +EF   L       N    R+  + D      +
Sbjct: 28  SVSEVEALYELFKSISSSVVDDGLINKEEFQLALFKNRKKENLFANRIFDLFDVKRKGVI 87

Query: 76  NFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
           +F DFV  L+ F   ASL  K +F F+LYD+DC G +   ++ ++L
Sbjct: 88  DFGDFVRSLNVFHPNASLEEKTDFTFRLYDMDCTGFIERQEVKQML 133

[Close]

BLAST of Carg09601 vs. TAIR 10
Match: AT3G22930.1 (calmodulin-like 11 )

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-05
Identity = 28/106 (26.42%), Postives = 55/106 (51.89%), Query Frame = 0


Query: 15  VSQQEIVSLFQKFCQLDRNSKGFISADEFLSV-PEFAMNPLPQRLLKMV-----DG---L 74
           ++Q++I+   + FC  D++  G I+ADE  +V      NP  Q L  M+     DG   +
Sbjct: 28  LTQEQIMEFKEAFCLFDKDGDGCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTI 87

Query: 75  NFKDFVAFLSAFSAKASLHHKIEFIFKLYDVDCNGKVSFNDIIEVL 112
            F +F+  ++    +     +++  FK++D D NG +S +++  V+
Sbjct: 88  EFSEFLNLMANQLQETDADEELKEAFKVFDKDQNGYISASELRHVM 133

The following BLAST results are available for this feature:

BLAST of Carg09601 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014153.1	2.2e-66	100.00	Calcineurin subunit B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022953695.1	1.8e-55	100.00	calcineurin subunit B-like isoform X2 [Cucurbita moschata]	[more]
XP_023548811.1	3.3e-54	98.25	calcineurin subunit B-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022991501.1	1.4e-52	85.61	calcineurin subunit B-like isoform X2 [Cucurbita maxima]	[more]
XP_038898822.1	1.1e-49	91.15	calcineurin subunit B-like isoform X2 [Benincasa hispida]	[more]

BLAST of Carg09601 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42322	6.8e-18	42.57	Calcineurin subunit B OS=Naegleria gruberi OX=5762 GN=CNB1 PE=3 SV=1	[more]
Q55G87	1.9e-15	35.64	Calcineurin subunit B type 1 OS=Dictyostelium discoideum OX=44689 GN=cnbA PE=1 S...	[more]
P0CM55	2.7e-14	36.44	Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (str...	[more]
P0CM54	2.7e-14	36.44	Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (str...	[more]
P87072	7.8e-14	37.00	Calcineurin subunit B OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS...	[more]

BLAST of Carg09601 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQD6	8.5e-56	100.00	calcineurin subunit B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1JQX3	6.8e-53	85.61	calcineurin subunit B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11148809...	[more]
A0A6J1GNZ0	1.7e-48	99.01	calcineurin subunit B-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1GP06	1.7e-48	99.01	calcineurin subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1GNT2	1.7e-48	99.01	calcineurin subunit B-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]

BLAST of Carg09601 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18430.1	2.8e-43	71.43	Calcium-binding EF-hand family protein	[more]
AT3G18430.2	2.8e-43	71.43	Calcium-binding EF-hand family protein	[more]
AT4G17615.1	3.3e-07	32.08	calcineurin B-like protein 1	[more]
AT5G47100.1	8.0e-06	29.25	calcineurin B-like protein 9	[more]
AT3G22930.1	1.0e-05	26.42	calmodulin-like 11	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 23..109 e-value: 2.6E-9 score: 37.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 18..53 score: 9.63171
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 82..117 score: 11.305434
None	No IPR available	GENE3D	1.10.238.10		coord: 4..130 e-value: 1.2E-22 score: 82.3
None	No IPR available	PANTHER	PTHR46971	CALCINEURIN B SUBUNIT (PROTEIN PHOSPHATASE 2B REGULATORY SUBUNIT)-LIKE PROTEIN	coord: 13..114
None	No IPR available	PANTHER	PTHR46971:SF1	CALCINEURIN B SUBUNIT (PROTEIN PHOSPHATASE 2B REGULATORY SUBUNIT)-LIKE PROTEIN	coord: 13..114
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 95..107
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 14..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09601-RA	Carg09601-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding