Carg09532 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09532
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
LocationCarg_Chr17: 6677569 .. 6680052 (+)
RNA-Seq ExpressionCarg09532
SyntenyCarg09532
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTTGCTATCAAAATGTTCAGAATGTTCTTCAAGCCGAAGTTCTATACAAAATGGTATTTCGCAGCTTTTCGTTTAACTTTTTCTCTCGGATTGATTTTGATTCTGATTCTTACTTTTCTGGCGGTTCTTCTTACTGATTTCTGTTTCCCTTTTTCATTCTAATGTAGCAAATCTTGCGTCAAGATGACGAAGACTCGTCTGGATACAATTCGGAAGAAGAAGAAAGTCGTTCTCAAGTTTTTGAAGAACGATATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCTTCTTGTCGAATCTGATTCTTAATCTCGGTTTTAGATTCTTGATCTCTGTTCGTCTTTGATTCTTTGTTAGTAAATGTAAAATCTATCAGAAACACATAATCTTAATGTCTTTTGTGTTCTGTGGTTGTTCTTAATTCTTTGTGTTCGGATGATTGTAAGAGAATTTTTGGTTTATTTTCTCTGGCTTTGGTCGCTAGTCTATGTGCTTATGCTAATGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCAAAATGTCTTGAGATGCTATGATCTGATTGATGAGTTTTGTGGGACAATCTTCAAGCACATTTCTGTCTTGAATAAGGAGAGGTAGATACCATTGATTTGTAAGTACATTTCTCTTTCTCAGGCTTGAATTTGTTGAGATGAATTTAAACGTGCTTCCCATGTTTATGTTTCTCTTCTAGCGAATGCCCCGACGAATGCAAAGAAGCGGTTGCGTCGTTGATTTACGCTGCAGCGAGATTCTCCGACTTGCCTGAATTGCGAGTGCTCCGAAGTCTCTTCACTGGGAGATATGGAACTTCTTTTGGATCATTTACTAACAAAGAGGTTGTTCTTGGTAACCCTTCTCTTGTTTGAGGGAGTTTCTTCTTTTTACTATCCTAAATGGTTGTTTAGTTCTTGCTTTTCCTTTTAGCTTGTTGAGAAGTTGAGGGCAGTCGCTCAAACTAAAGAAACAAAGCTTCAGCTACTCCAGGAGATAGCACAGGAATCCGCCATTGATTGGAATTCCAAAGCTCTGGAACAGCAACTGTATGTTCCTCCACAAAATGAAGTAAGTTCGTTTCCACGTATCTGATCTTGTTTGTTTTATGAATCACGACTCTCCATAATCGTATGATATTATCCACTTTGAGCATAAGTTTTCGTGGCTTTATTTTTTGTTTCACCAATAAGGTCTCGTACGAATGGAGATGTATTCCTTACTTATAAACTCATGATCATTCCCCTCGAACGAAAGTACACCATAGAGCCTTTGTACAGCCTCCCCTTAATCGAGGCTCGACTCCTTGAATGGAGTTCGATGAAAATGATCCATTTTCTTTATCTGTTTGTGTTGATCTCTCTTCATCCTATTGATGGTTCTATCTACTGCAATGGTCATTCTCTTCACAGCTAGATCGTGAACGGAGTACATCCACCAATCGAAGCAAAAGTAAGATCGTTTCGGTTCCGGTGTACGGTAGAACGACGAACTCACCAAGAAAAAAGGACAATAGTGACGACGATTCCATCTTCGATAGTAGAAGTGAAGGCAACACGACCGAAACCTCGATGGGAGATAGTAGTACCGATCAAGATGTTCATAAAGGTGTTGTTTCAGAGGATGAAGTAGAAGATCAGAAACCTTTCTACCTTAGATTTATCACACCTCCCTACCTTAAAACAAAACCTACCAAAAAGGAAGAAAATGTGGAGGAACCTCAAAACGTTACCGAAGAGAACCGAAACGATCCAAAGACAGCCATAGAAGAGAAGCCGAAACCGAGATCGGTGAGGCGAAGACATGCGAAACCACAGCCTGCAAGGAGCATCAACATCGACGATGCTGGTGGTTCGGCAGCCGACGGTGCAGGAAAGATTAGTTCGGGTCGAAACAAAGGTAAAGAAGCCATGACAGGAGAAGAAAAAGGTGTAGAGGATGATGAGGAAAGGATGTTAGATGGGCTTTTGAAGCTATACAGTAAGAAGAAAACAAGACAGGAAGACAAAGACAATGCCGAACCTCAAAGGCCCAAAAACACAAGAACTGAGGACGCTTATGTCCCAACCAAATCATTGTCACTTCCCCCACCCCGAACCGAGCCGATCGAGCCAGTGAAAAAGCACGCTAGAGCGAATTCCTACGTTCATCCAAAGCTCCCGGACTACGACCAGCTCGCAGCTCGGTTTGCAGCGCTCAAAGAGAAGTAAACAACGTCGAAATCGAACAAGACCAATTGCACACTTGCAATTACAATGTGTAGCTTGAAGAAAAGGCAGTAAAATTGATGTAAAAAGGGTGTAATATGATGATGATGATCCACGTTGATTGTGATATTTTCAGAGCCATTTTTTTTAGTTAACTTTAATCTTGGTTACAACAACAGAGAGATTATGAAGTAATTGGGTTGTCGGAGAATCTACGACAAGAAGGAAGACAACATTG

mRNA sequence

ACTTTGCTATCAAAATGTTCAGAATGTTCTTCAAGCCGAAGTTCTATACAAAATGCAAATCTTGCGTCAAGATGACGAAGACTCGTCTGGATACAATTCGGAAGAAGAAGAAAGTCGTTCTCAAGTTTTTGAAGAACGATATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCAAAATGTCTTGAGATGCTATGATCTGATTGATGAGTTTTGTGGGACAATCTTCAAGCACATTTCTGTCTTGAATAAGGAGAGCGAATGCCCCGACGAATGCAAAGAAGCGGTTGCGTCGTTGATTTACGCTGCAGCGAGATTCTCCGACTTGCCTGAATTGCGAGTGCTCCGAAGTCTCTTCACTGGGAGATATGGAACTTCTTTTGGATCATTTACTAACAAAGAGCTTGTTGAGAAGTTGAGGGCAGTCGCTCAAACTAAAGAAACAAAGCTTCAGCTACTCCAGGAGATAGCACAGGAATCCGCCATTGATTGGAATTCCAAAGCTCTGGAACAGCAACTGTATGTTCCTCCACAAAATGAACTAGATCGTGAACGGAGTACATCCACCAATCGAAGCAAAAGTAAGATCGTTTCGGTTCCGGTGTACGGTAGAACGACGAACTCACCAAGAAAAAAGGACAATAGTGACGACGATTCCATCTTCGATAGTAGAAGTGAAGGCAACACGACCGAAACCTCGATGGGAGATAGTAGTACCGATCAAGATGTTCATAAAGGTGTTGTTTCAGAGGATGAAGTAGAAGATCAGAAACCTTTCTACCTTAGATTTATCACACCTCCCTACCTTAAAACAAAACCTACCAAAAAGGAAGAAAATGTGGAGGAACCTCAAAACGTTACCGAAGAGAACCGAAACGATCCAAAGACAGCCATAGAAGAGAAGCCGAAACCGAGATCGGTGAGGCGAAGACATGCGAAACCACAGCCTGCAAGGAGCATCAACATCGACGATGCTGGTGGTTCGGCAGCCGACGGTGCAGGAAAGATTAGTTCGGGTCGAAACAAAGGTAAAGAAGCCATGACAGGAGAAGAAAAAGGTGTAGAGGATGATGAGGAAAGGATGTTAGATGGGCTTTTGAAGCTATACAGTAAGAAGAAAACAAGACAGGAAGACAAAGACAATGCCGAACCTCAAAGGCCCAAAAACACAAGAACTGAGGACGCTTATGTCCCAACCAAATCATTGTCACTTCCCCCACCCCGAACCGAGCCGATCGAGCCAGTGAAAAAGCACGCTAGAGCGAATTCCTACGTTCATCCAAAGCTCCCGGACTACGACCAGCTCGCAGCTCGGTTTGCAGCGCTCAAAGAGAAGTAAACAACGTCGAAATCGAACAAGACCAATTGCACACTTGCAATTACAATGTGTAGCTTGAAGAAAAGGCAGTAAAATTGATGTAAAAAGGGTGTAATATGATGATGATGATCCACGTTGATTGTGATATTTTCAGAGCCATTTTTTTTAGTTAACTTTAATCTTGGTTACAACAACAGAGAGATTATGAAGTAATTGGGTTGTCGGAGAATCTACGACAAGAAGGAAGACAACATTG

Coding sequence (CDS)

ATGTTCAGAATGTTCTTCAAGCCGAAGTTCTATACAAAATGCAAATCTTGCGTCAAGATGACGAAGACTCGTCTGGATACAATTCGGAAGAAGAAGAAAGTCGTTCTCAAGTTTTTGAAGAACGATATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCAAAATGTCTTGAGATGCTATGATCTGATTGATGAGTTTTGTGGGACAATCTTCAAGCACATTTCTGTCTTGAATAAGGAGAGCGAATGCCCCGACGAATGCAAAGAAGCGGTTGCGTCGTTGATTTACGCTGCAGCGAGATTCTCCGACTTGCCTGAATTGCGAGTGCTCCGAAGTCTCTTCACTGGGAGATATGGAACTTCTTTTGGATCATTTACTAACAAAGAGCTTGTTGAGAAGTTGAGGGCAGTCGCTCAAACTAAAGAAACAAAGCTTCAGCTACTCCAGGAGATAGCACAGGAATCCGCCATTGATTGGAATTCCAAAGCTCTGGAACAGCAACTGTATGTTCCTCCACAAAATGAACTAGATCGTGAACGGAGTACATCCACCAATCGAAGCAAAAGTAAGATCGTTTCGGTTCCGGTGTACGGTAGAACGACGAACTCACCAAGAAAAAAGGACAATAGTGACGACGATTCCATCTTCGATAGTAGAAGTGAAGGCAACACGACCGAAACCTCGATGGGAGATAGTAGTACCGATCAAGATGTTCATAAAGGTGTTGTTTCAGAGGATGAAGTAGAAGATCAGAAACCTTTCTACCTTAGATTTATCACACCTCCCTACCTTAAAACAAAACCTACCAAAAAGGAAGAAAATGTGGAGGAACCTCAAAACGTTACCGAAGAGAACCGAAACGATCCAAAGACAGCCATAGAAGAGAAGCCGAAACCGAGATCGGTGAGGCGAAGACATGCGAAACCACAGCCTGCAAGGAGCATCAACATCGACGATGCTGGTGGTTCGGCAGCCGACGGTGCAGGAAAGATTAGTTCGGGTCGAAACAAAGGTAAAGAAGCCATGACAGGAGAAGAAAAAGGTGTAGAGGATGATGAGGAAAGGATGTTAGATGGGCTTTTGAAGCTATACAGTAAGAAGAAAACAAGACAGGAAGACAAAGACAATGCCGAACCTCAAAGGCCCAAAAACACAAGAACTGAGGACGCTTATGTCCCAACCAAATCATTGTCACTTCCCCCACCCCGAACCGAGCCGATCGAGCCAGTGAAAAAGCACGCTAGAGCGAATTCCTACGTTCATCCAAAGCTCCCGGACTACGACCAGCTCGCAGCTCGGTTTGCAGCGCTCAAAGAGAAGTAA

Protein sequence

MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKGVEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK
Homology
BLAST of Carg09532 vs. NCBI nr
Match: KAG7014085.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863.2 bits (2229), Expect = 9.8e-247
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
           VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFAALKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Carg09532 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 860.5 bits (2222), Expect = 6.4e-246
Identity = 450/452 (99.56%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNELDRERSTSTNR+KSKIVSVPVYGRTTNSPRKK+NSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELDRERSTSTNRNKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
           VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFAALKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Carg09532 vs. NCBI nr
Match: XP_022953212.1 (uncharacterized protein LOC111455825 [Cucurbita moschata])

HSP 1 Score: 847.4 bits (2188), Expect = 5.6e-242
Identity = 446/452 (98.67%), Postives = 447/452 (98.89%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFT RYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNELD ERSTST RSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQN TEENRND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISS RNKGKEAMTGEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
           VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFA+LKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Carg09532 vs. NCBI nr
Match: XP_023547751.1 (uncharacterized protein LOC111806608 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-239
Identity = 441/452 (97.57%), Postives = 443/452 (98.01%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIF HISVLNKESECPDECKEAVASLIY+AARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYSAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFT RYGTSFGSFTNKELVEKLRAVAQTKETKL LLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLHLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNELDRERSTSTNR KSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELDRERSTSTNRCKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEE VEEPQNVTEE RND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEETVEEPQNVTEEKRND 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKTAIEEKPKPRSVRRRHAKPQPARSINIDD GGSAADGAGKISSGRNKGKEAMTGEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDDGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
           VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTE+AYVPTKSLSLP PRTEPIE
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEEAYVPTKSLSLPTPRTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAAR AALKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARIAALKEK 452

BLAST of Carg09532 vs. NCBI nr
Match: XP_022992255.1 (uncharacterized protein LOC111488629 [Cucurbita maxima])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 435/452 (96.24%), Postives = 441/452 (97.57%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIF HISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFT RYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNEL RERSTSTNRSKSKIVSVPVYGRTTNSP+KKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKG+VSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEE RN+
Sbjct: 241 MGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEKRNN 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKT  EEKPKPRSVRRRHAKPQPARSINIDD+GGSAADGAGKISSG+NKGKEAMTGEEKG
Sbjct: 301 PKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
            EDDEERMLDGLLK+YSKK TRQEDKDNAEPQRPKNTRTEDAYVP KSLSLP  RTEPIE
Sbjct: 361 EEDDEERMLDGLLKIYSKKNTRQEDKDNAEPQRPKNTRTEDAYVPAKSLSLPTARTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFAALKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Carg09532 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.0e-12
Identity = 42/167 (25.15%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 13  KCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCY 72
           K K  +K+  +R+  ++ KK  +++  K ++ ELL+ + + +A  R E  + ++ ++ C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  DLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTGRYGT 132
            +I+  C  +   I+++N  +E P E KE++ +L+Y++ R   +PEL  +++    +YG 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 SF--------GSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDW 172
                      +  N ++V KL           Q L EIA++  +DW
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of Carg09532 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 2.7e-242
Identity = 446/452 (98.67%), Postives = 447/452 (98.89%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFT RYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNELD ERSTST RSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQN TEENRND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISS RNKGKEAMTGEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
           VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFA+LKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Carg09532 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.8e-237
Identity = 435/452 (96.24%), Postives = 441/452 (97.57%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVEQNVLRCYDLIDEFCGTIF HISVLNKESECPDECKEAVASLIYAAARFSDLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
           VLRSLFT RYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240
           YVPPQNEL RERSTSTNRSKSKIVSVPVYGRTTNSP+KKDNSDDDSIFDSRSEGNTTETS
Sbjct: 181 YVPPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300
           MGDSSTDQDVHKG+VSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEE RN+
Sbjct: 241 MGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEKRNN 300

Query: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360
           PKT  EEKPKPRSVRRRHAKPQPARSINIDD+GGSAADGAGKISSG+NKGKEAMTGEEKG
Sbjct: 301 PKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGEEKG 360

Query: 361 VEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTEPIE 420
            EDDEERMLDGLLK+YSKK TRQEDKDNAEPQRPKNTRTEDAYVP KSLSLP  RTEPIE
Sbjct: 361 EEDDEERMLDGLLKIYSKKNTRQEDKDNAEPQRPKNTRTEDAYVPAKSLSLPTARTEPIE 420

Query: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           PVKKHARANSYVHPKLPDYDQLAARFAALKEK
Sbjct: 421 PVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Carg09532 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.8e-170
Identity = 339/460 (73.70%), Postives = 375/460 (81.52%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MF MF KPKFYTKCKSCVKMTKTRLDTIRKKK  VLK+LKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI   I VLNKESECPDECKEAVA+LIYAAARF+DLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
            LR+LFT +YG+SFGSFTNKE +EK RA  QTKE K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YV-PPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTET 240
           Y  PPQNELD ERS +T R+K+K+VSVPVY R  NSPR K+NSD++SIFDSRSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 SMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEN-- 300
           S GD STDQD+HKG VSEDEVEDQKPF  RF+ PPYLKTKP K E N +EP+ VT EN  
Sbjct: 241 STGD-STDQDIHKG-VSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDE 300

Query: 301 RNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGE 360
            N+PK+  EEKPKPRSVRRR+ KPQPAR INIDD G S  D   KISS RNKGKE M GE
Sbjct: 301 SNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGE 360

Query: 361 EKGVEDDEERMLDGLLKLYSKKKTRQED----KDNAEPQRPKNTRTEDAYVP-TKSLSLP 420
           EKG  DDEER+LDGLL  YSKKKT QE     K N +PQR +     +   P T+++SLP
Sbjct: 361 EKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLP 420

Query: 421 PPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
              T+  E  KKH R NS+VHPKLP+YDQLAAR AALKEK
Sbjct: 421 ---TDQNESTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Carg09532 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 4.6e-165
Identity = 334/460 (72.61%), Postives = 371/460 (80.65%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MF MF KPKFYTKCKSCVKMTKTRLDT RKKK  VLK+LKNDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI   I VLNKESECPDECKE+VA+LIYAAARF+DLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
            LR+LFT +YG+SFGSFTNKE +EK R   QTKE K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YV-PPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTET 240
           Y  PP+NELD ERS +T R+K+K+VSVPVY +  NSPR K+NSD++SIFDSRSEGNTTET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 SMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEE-PQNVTEEN- 300
           S GD STDQDVHKG VS DEV DQKPF  RF+ PPYLKTKP K E N EE P+ VT EN 
Sbjct: 241 STGD-STDQDVHKG-VSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIEND 300

Query: 301 RNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGE 360
            ++PK+  EEKPKPRSVRRR  KPQPAR INIDD G S  D   KISS RNKGKE M GE
Sbjct: 301 ESNPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGE 360

Query: 361 EKGVEDDEERMLDGLLKLYSKKKTRQED----KDNAEPQRPKNTRTEDAYVPT-KSLSLP 420
           EKG  DDEER+LDGLL  YSKKKT QE     K N +PQR +     +   PT +++S P
Sbjct: 361 EKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSFP 420

Query: 421 PPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 453
           P +    EP+KKH R NS+VHPKLP+YDQLAAR AALKEK
Sbjct: 421 PDQN---EPMKKHTRTNSFVHPKLPEYDQLAARIAALKEK 454

BLAST of Carg09532 vs. ExPASy TrEMBL
Match: A0A5D3BX00 (Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002360 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 5.8e-160
Identity = 322/441 (73.02%), Postives = 358/441 (81.18%), Query Frame = 0

Query: 20  MTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCYDLIDEFC 79
           MTKTRLDTIRKKK  VLK+LKNDIVELLKSRLDYNA+NRAEGFLVE+NVLRCY+LIDEFC
Sbjct: 1   MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 60

Query: 80  GTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTGRYGTSFGSFTN 139
           GTI   I VLNKESECPDECKEAVA+LIYAAARF+DLPELR LR+LFT +YG+SFGSFTN
Sbjct: 61  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120

Query: 140 KELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYV-PPQNELDRERSTSTNR 199
           KE +EK RA  QTKE K+QLLQEIAQE+AIDWNSKALEQQLY  PPQNELD ERS +T R
Sbjct: 121 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 180

Query: 200 SKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGVVSED 259
           +K+K+VSVPVY R  NSPR K+NSD++SIFDSRSEGNTTETS GD STDQD+HKG VSED
Sbjct: 181 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGD-STDQDIHKG-VSED 240

Query: 260 EVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEN--RNDPKTAIEEKPKPRSVRR 319
           EVEDQKPF  RF+ PPYLKTKP K E N +EP+ VT EN   N+PK+  EEKPKPRSVRR
Sbjct: 241 EVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRR 300

Query: 320 RHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKGVEDDEERMLDGLLKLY 379
           R+ KPQPAR INIDD G S  D   KISS RNKGKE M GEEKG  DDEER+LDGLL  Y
Sbjct: 301 RNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQY 360

Query: 380 SKKKTRQED----KDNAEPQRPKNTRTEDAYVP-TKSLSLPPPRTEPIEPVKKHARANSY 439
           SKKKT QE     K N +PQR +     +   P T+++SLP   T+  E  KKH R NS+
Sbjct: 361 SKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLP---TDQNESTKKHTRTNSF 420

Query: 440 VHPKLPDYDQLAARFAALKEK 453
           VHPKLP+YDQLAAR AALKEK
Sbjct: 421 VHPKLPEYDQLAARIAALKEK 436

BLAST of Carg09532 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.8e-66
Identity = 155/329 (47.11%), Postives = 213/329 (64.74%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MF   FKPKFYTKCKS VK+TKTR+DT+++KK  V K+LKNDIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           G + E+  L CY+ +++FC  +  ++S+L K   CPDEC+EA++SL+YAAAR S++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
            LRSLF  RYG +   F N E VE+ +A   +KE K++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YVPPQN-----ELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGN 240
           Y PP +     E +  ++  TN    K +      R++ S   +++S DDS   ++S  +
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTME----KRSSLSFHGREDSMDDS---NKSIMS 240

Query: 241 TTETSMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITP-PYLKTKPTKKEENVEEPQNVT 300
           T+E      ST      G   ED+ E  KPFY RF+TP PY K K  K+E     P+ +T
Sbjct: 241 TSEED--SMSTSGSCMTG--PEDDPE-MKPFYYRFMTPAPYTKPKIEKQE---SLPEKMT 300

Query: 301 EENRNDPKTAIEEKPKPRSVRRRHAKPQP 324
             +  D ++    KPKPRSVRRR A P P
Sbjct: 301 TSHLMDTESPTGGKPKPRSVRRRFANPPP 314

BLAST of Carg09532 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-42
Identity = 113/270 (41.85%), Postives = 163/270 (60.37%), Query Frame = 0

Query: 60  EGFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPEL 119
           EG + E+  L CY+ +++FC  +  ++S+L K   CPDEC+EA++SL+YAAAR S++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RVLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQ 179
           R LRSLF  RYG +   F N E VE+ +A   +KE K++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYVPPQN-----ELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEG 239
           LY PP +     E +  ++  TN    K +      R++ S   +++S DDS   ++S  
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTME----KRSSLSFHGREDSMDDS---NKSIM 225

Query: 240 NTTETSMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITP-PYLKTKPTKKEENVEEPQNV 299
           +T+E      ST      G   ED+ E  KPFY RF+TP PY K K  K+E     P+ +
Sbjct: 226 STSEED--SMSTSGSCMTG--PEDDPE-MKPFYYRFMTPAPYTKPKIEKQE---SLPEKM 285

Query: 300 TEENRNDPKTAIEEKPKPRSVRRRHAKPQP 324
           T  +  D ++    KPKPRSVRRR A P P
Sbjct: 286 TTSHLMDTESPTGGKPKPRSVRRRFANPPP 300

BLAST of Carg09532 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-35
Identity = 108/310 (34.84%), Postives = 169/310 (54.52%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MF   FKPKFY K KS     K R+D +R+K+  +++  K DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
             L E  ++ CYDLI+ FC  I +++S++ K+ ECP+EC+EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAV-AQTKETKLQLLQEIAQESAIDWNSKALEQQ 180
            LR++FT R+G    S  N ELVEK   +   ++E K+Q ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYVPPQNELDRERSTSTNRSKSKIVSVPVY-GRTTNSPRKKDNSDDDSI------FDSRS 240
           L           R TS  + K K+ +   Y  + T      ++SDD+S+       DS S
Sbjct: 181 LL----------RETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLS 240

Query: 241 EGNTTETSMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKT---KPTKKEENVEE 300
           +G+ + +S   SS+ +      VS  + + +K      I+ P  K       K +E  EE
Sbjct: 241 KGSLSSSSSSSSSSLRR-----VSVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEE 295

BLAST of Carg09532 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-32
Identity = 92/288 (31.94%), Postives = 155/288 (53.82%), Query Frame = 0

Query: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60
           MF  F    F  K K  +K+TK R+D +R+K+   +KFLK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120
           G L E   L   D +++ C  ++K +S + K  ECP++C+EA++SL++AA+ FS+LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180
            LR +F  +Y  S   F N+ELVE + +   + E K++L++++A E +I W+SK  E++ 
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKR- 180

Query: 181 YVPPQNELDRERSTSTNRSKSKIV-----------------SVPVYGRTTNSPRKKD--- 240
            +  QN +    +  +   K K V                  V +  +T  +  ++D   
Sbjct: 181 -IVRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLF 240

Query: 241 NSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGVVSEDEVEDQKPFYL 269
            SD +S + +   GN    +  + S +        S+D   ++K FYL
Sbjct: 241 QSDKES-YQNGLRGNQRGLTYKERSENVLHASRSESKDNKAERKEFYL 285

BLAST of Carg09532 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 116.7 bits (291), Expect = 4.8e-26
Identity = 123/456 (26.97%), Postives = 202/456 (44.30%), Query Frame = 0

Query: 12  TKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRC 71
           +KCK+  KM   R+  IR K+ VV+K ++ DI  LL+S  D  A  R E  + EQN+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTGRYG 131
            ++I+ FC  I   ++++ K+ +CP + KE +ASLI+AA R S++PEL  LR +F  +YG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 TSFGSF---------TNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYV 191
             F S           N+ L++KL       E KL++++EIA+E  +DW++   EQ+L  
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLK 208

Query: 192 PPQNELDRER---STSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTET 251
           P +  +D  R   S S+       ++ P+   T   PR   +   ++ +          T
Sbjct: 209 PQEESIDGPRKFVSASSLPVNRAAINEPI-DPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 SMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRN 311
            +   +        +++       K F +   +      K ++  ++         ++R+
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVS--SDHSTHQKDSQYMDHHHHHPGSRRQSRD 328

Query: 312 DPKTAIEEKP--KPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGE 371
              ++   KP  + R + RRH+   P         G + +D   + ++   + KE M   
Sbjct: 329 SETSSYYAKPGAENRGMGRRHSYNNP---------GINESDYEEEYTNTEAEAKETMRRR 388

Query: 372 EKGVEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPPRTE 431
                          +K        +E     EP  P   R           SLPP R  
Sbjct: 389 HSYNPRSVPPPATSEIKFDESDYYEEE----TEPDEPSQGRVS---------SLPPNRAP 448

Query: 432 PIEPVKKHARANS---YVHPKLPDYDQLAARFAALK 451
           P  P    +R +S    VHPKLPDYD LAARF A++
Sbjct: 449 PQAPQSGESRQDSSGHQVHPKLPDYDILAARFEAIR 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014085.19.8e-247100.00IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6575541.16.4e-24699.56IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022953212.15.6e-24298.67uncharacterized protein LOC111455825 [Cucurbita moschata][more]
XP_023547751.11.5e-23997.57uncharacterized protein LOC111806608 [Cucurbita pepo subsp. pepo][more]
XP_022992255.11.2e-23696.24uncharacterized protein LOC111488629 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q54I393.0e-1225.15IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GMM12.7e-24298.67uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC1114558... [more]
A0A6J1JV695.8e-23796.24uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629... [more]
A0A1S3CGT52.8e-17073.70uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A0A0K9264.6e-16572.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1[more]
A0A5D3BX005.8e-16073.02Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo v... [more]
Match NameE-valueIdentityDescription
AT1G79910.12.8e-6647.11Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.24.8e-4241.85Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.11.2e-3534.84Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.13.5e-3231.94Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.14.8e-2626.97Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..397
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..260
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 9..452
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 15..169
e-value: 9.7E-44
score: 149.2
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 9..452
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..177
e-value: 5.4E-49
score: 168.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09532-RACarg09532-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport