Carg09228 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09228
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	eukaryotic translation initiation factor 5-like
Location	Carg_Chr05: 2466510 .. 2467859 (-)
RNA-Seq Expression	Carg09228
Synteny	Carg09228

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

mRNA sequence

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Coding sequence (CDS)

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCGAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAAAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGATGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAACGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAAGTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMINMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKIKKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE

Homology

[Close]

BLAST of Carg09228 vs. NCBI nr
Match: KAG7029612.1 (Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.5 bits (2209), Expect = 2.0e-244
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 449

[Close]

BLAST of Carg09228 vs. NCBI nr
Match: XP_023546001.1 (eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP_023546002.1 eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 6.5e-243
Identity = 447/449 (99.55%), Postives = 447/449 (99.55%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAADDDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAADDDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEE VLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEIVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 449

[Close]

BLAST of Carg09228 vs. NCBI nr
Match: KAG6598667.1 (Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA DDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA-DDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKK+PSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. NCBI nr
Match: XP_022962417.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_022962418.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata])

HSP 1 Score: 845.9 bits (2184), Expect = 1.6e-241
Identity = 446/449 (99.33%), Postives = 446/449 (99.33%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA DDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA-DDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNT PE AKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. NCBI nr
Match: XP_022997142.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022997143.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima])

HSP 1 Score: 837.8 bits (2163), Expect = 4.4e-239
Identity = 442/449 (98.44%), Postives = 446/449 (99.33%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAA DDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAA-DDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGN VS+HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNRVSSHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LV+EIKDYLK+SPSAADLKSFLESLTGTHQETANAL+EALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVNEIKDYLKRSPSAADLKSFLESLTGTHQETANALIEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. ExPASy Swiss-Prot
Match: P48724 (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.1e-159
Identity = 313/454 (68.94%), Postives = 362/454 (79.74%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGA N DDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAK L RPA+YTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG + VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEELKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDSLKKKSTS---GNAKEAVTKVPSKKKSNGSDED-HSPTRSHAVENDQAADDDDDDDD 240
           KK+  KK S+S   G AK  +    SKKK +GSDED  SPT     E ++A D+DDDDDD
Sbjct: 181 KKEVKKKGSSSAKDGTAKSTI----SKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDD 240

Query: 241 -VQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGV 300
            VQW TDTSL+A++QRI+EQLSAVTADMVML TT+E +  KK+  ++     GS+NGN  
Sbjct: 241 GVQWLTDTSLDASRQRIKEQLSAVTADMVML-TTDEPEKKKKAASNQN---GGSQNGNS- 300

Query: 301 STHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKK 360
             +G +V E+K  LKK   A++L S L +L    QE  +ALVEALFEG  KGF +E +KK
Sbjct: 301 KNYGTVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKK 360

Query: 361 KKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWY 420
           K +  A  A  EEGSQI+LL +IE FC   +  A KE+ALVLK+LYD DV+EEE ++ WY
Sbjct: 361 KNYFAAAVA--EEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWY 420

Query: 421 QQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           Q+GL G NK S+IWK+ +PFI+WLQNAESE++EE
Sbjct: 421 QKGLKGDNKNSKIWKNAQPFIDWLQNAESESDEE 443

[Close]

BLAST of Carg09228 vs. ExPASy Swiss-Prot
Match: Q9C8F1 (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 8.2e-156
Identity = 308/460 (66.96%), Postives = 365/460 (79.35%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE++K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKD--SLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQ 240
           KK+  S KK +T+G +K+ V    SKKK      DHSP RS + ENDQ AD ++DDDDVQ
Sbjct: 181 KKEAASKKKAATTGTSKDKV----SKKK------DHSPPRSLSDENDQ-ADSEEDDDDVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG- 300
           WQTDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN  
Sbjct: 241 WQTDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSK 300

Query: 301 --VSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEV 360
              + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV
Sbjct: 301 IPENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEV 360

Query: 361 VKKKKFLVAVAAAQEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEE 420
           +KKKK+L+A+   QEE    +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+
Sbjct: 361 IKKKKYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDED 420

Query: 421 FVLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
            ++EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 VIVEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Carg09228 vs. ExPASy Swiss-Prot
Match: P55876 (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-155
Identity = 307/457 (67.18%), Postives = 361/457 (78.99%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVDIAKAL RPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGI+LVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDH--SPTRSHAVENDQAADDDDDDDDVQ 240
           KKD+ KK S    AK    K  +   + GSDEDH  SPTRSH  + D+AA  DDDDDDVQ
Sbjct: 181 KKDAKKKGSKDSTAKGLKKKATT---ATGSDEDHSSSPTRSH--DGDKAA-ADDDDDDVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNG------ 300
           WQTDTS+EAAKQR+QEQLSA TA+MVMLS TEET+   K P H+    NGS         
Sbjct: 241 WQTDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKP 300

Query: 301 --NGVSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSK 360
                S +  L+ +IK  L  +P+ + LK+ L S T   Q+  NA +EALF GVGKGF+K
Sbjct: 301 AVTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTK 360

Query: 361 EVVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEF 420
           EVVK KK+L    A  +EG+Q +L+++IE+F    +PEA KE+ +VLK+LYDGD+++EE 
Sbjct: 361 EVVKNKKYL--AVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEET 420

Query: 421 VLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETE 448
           +++WY   +A A K SQ+ K+ KPF+EWLQ+AES+ E
Sbjct: 421 IVDWYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

[Close]

BLAST of Carg09228 vs. ExPASy Swiss-Prot
Match: Q9S825 (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.7e-151
Identity = 297/451 (65.85%), Postives = 355/451 (78.71%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEE +K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTR-SHAVENDQAADDDDDDDDVQW 240
           K     KK    N K++ T      K++ SDED SP    +A+E D   +D+DDDD V+W
Sbjct: 181 K----AKKKALSNGKDSKT-----SKNHSSDEDISPKHDENALEVD---EDEDDDDGVEW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHG 300
           QTDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N      
Sbjct: 241 QTDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEK 300

Query: 301 ALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFL 360
            LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV KKK +L
Sbjct: 301 NLVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYL 360

Query: 361 VAVAAAQEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQG 420
            A A  QE+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++G
Sbjct: 361 AAAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKG 420

Query: 421 LAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           L GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 LTGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Carg09228 vs. ExPASy Swiss-Prot
Match: P59325 (Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.2e-47
Identity = 148/450 (32.89%), Postives = 213/450 (47.33%), Query Frame = 0


Query: 13  DAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEK 72
           D FYRYKMPR++ K+EG+GNGIKT +VNMVD+AKAL RP  Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMINMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKIKKDSLKKKSTS 192
           +D   KL TFILKNPPE       KK     EKE                     KK+  
Sbjct: 131 LDTHHKLCTFILKNPPENSDIGTGKK-----EKE---------------------KKNRK 190

Query: 193 GNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQTDTSLEAAKQR 252
           G  KE          S  + E   P   + +    A ++++DDD   W  DT+ EA ++R
Sbjct: 191 GKDKE--------NGSVSTSETPPPPPPNEISPPHAVEEEEDDD---WGEDTTEEAQRRR 250

Query: 253 IQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGALVHEIKDYLKK 312
           + E              ++  K    S + E++V                V+ + D++KK
Sbjct: 251 MDE-------------ISDHAKGLTLSDDLERTVEE-------------RVNILFDFVKK 310

Query: 313 SPSAADLKSFLESLTGTHQET------ANALVEALFEGVGKGFSKEVVKKKKFLVAVAAA 372
                 + S  + +    +           L E LF+       +E +KK +        
Sbjct: 311 KKEEGIIDSSDKEIVAEAERLDVKAMGPLVLTEVLFDEK----IREQIKKYRRHFLRFCH 370

Query: 373 QEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQG---LAGA 432
             + +Q  LL  +E        +   +I  +LK +YD D++EEE ++ W ++        
Sbjct: 371 NNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSK 393

Query: 433 NKGSQIWKHVKPFIEWLQNAESET---EEE 450
               +I    +PFI+WL+ AE E+   EEE
Sbjct: 431 ELAKEIRVKAEPFIKWLKEAEEESSGGEEE 393

[Close]

BLAST of Carg09228 vs. ExPASy TrEMBL
Match: A0A6J1HCN3 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111462865 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.8e-242
Identity = 446/449 (99.33%), Postives = 446/449 (99.33%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA DDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAA-DDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNT PE AKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. ExPASy TrEMBL
Match: A0A6J1K464 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita maxima OX=3661 GN=LOC111492137 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.1e-239
Identity = 442/449 (98.44%), Postives = 446/449 (99.33%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAA DDDDDDDVQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAA-DDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGN VS+HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNRVSSHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LV+EIKDYLK+SPSAADLKSFLESLTGTHQETANAL+EALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVNEIKDYLKRSPSAADLKSFLESLTGTHQETANALIEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. ExPASy TrEMBL
Match: A0A6J1BQ30 (eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 GN=LOC111004721 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.0e-217
Identity = 398/449 (88.64%), Postives = 423/449 (94.21%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKDS+KKKST+GN K+AVTKVPSKKK+NGSDEDHSPTRS A +ND AADDD+D+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS E+EKS VNG+KNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKK  SAA+LKSFLESL+GT QE  NALVE LF+GVGKGFSKEV+KKK FL 
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFL- 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           A AAAQE  SQI+LLR+IES CLNT+PEA KE+ALVLKSLYDGDV+EEEFVLEWYQQGLA
Sbjct: 361 AAAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           G NK SQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GENKSSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Carg09228 vs. ExPASy TrEMBL
Match: A0A0A0LMB4 (W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.5e-216
Identity = 402/449 (89.53%), Postives = 420/449 (93.54%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +ND+AA DD+DDDDVQWQ
Sbjct: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAA-DDEDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           L+ EIKDYLKK  S  DLKSFLESL GT QE  NALVEALF+GVGKGFSKEVVKKKKFL 
Sbjct: 301 LIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
            VAA   EGSQI+LLR+IESFCLNTSPEA KE+ALVLKSLYDGDVIEEEFVLEWYQQG+A
Sbjct: 361 VVAAV--EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Carg09228 vs. ExPASy TrEMBL
Match: A0A1S3BCI3 (eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC103488416 PE=3 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 8.1e-215
Identity = 400/449 (89.09%), Postives = 420/449 (93.54%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +NDQAA DD+DDDDVQWQ
Sbjct: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAA-DDEDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTH +
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVS 300

Query: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           L+ EIKD+LKK  S  DLKSFLESL+GT QE  NALVEALF+GVGKGFSKEVVKKKKFL 
Sbjct: 301 LIQEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
            VAA  EEGSQI LLRSIESFC+NTSPEA KE+ALVLKSLYDGDVIEEEFVLEWY+QG+A
Sbjct: 361 VVAA--EEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 450
           GANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Carg09228 vs. TAIR 10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 551.6 bits (1420), Expect = 5.9e-157
Identity = 308/460 (66.96%), Postives = 365/460 (79.35%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE++K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKD--SLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQ 240
           KK+  S KK +T+G +K+ V    SKKK      DHSP RS + ENDQ AD ++DDDDVQ
Sbjct: 181 KKEAASKKKAATTGTSKDKV----SKKK------DHSPPRSLSDENDQ-ADSEEDDDDVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG- 300
           WQTDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN  
Sbjct: 241 WQTDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSK 300

Query: 301 --VSTHGALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEV 360
              + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV
Sbjct: 301 IPENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEV 360

Query: 361 VKKKKFLVAVAAAQEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEE 420
           +KKKK+L+A+   QEE    +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+
Sbjct: 361 IKKKKYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDED 420

Query: 421 FVLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
            ++EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 VIVEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Carg09228 vs. TAIR 10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 536.6 bits (1381), Expect = 1.9e-152
Identity = 297/451 (65.85%), Postives = 355/451 (78.71%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEE +K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTR-SHAVENDQAADDDDDDDDVQW 240
           K     KK    N K++ T      K++ SDED SP    +A+E D   +D+DDDD V+W
Sbjct: 181 K----AKKKALSNGKDSKT-----SKNHSSDEDISPKHDENALEVD---EDEDDDDGVEW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHG 300
           QTDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N      
Sbjct: 241 QTDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEK 300

Query: 301 ALVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFL 360
            LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV KKK +L
Sbjct: 301 NLVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYL 360

Query: 361 VAVAAAQEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQG 420
            A A  QE+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++G
Sbjct: 361 AAAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKG 420

Query: 421 LAGANKGSQIWKHVKPFIEWLQNAESETEEE 450
           L GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 LTGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Carg09228 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

[Close]

BLAST of Carg09228 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 156 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 215

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 216 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 246

[Close]

BLAST of Carg09228 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

The following BLAST results are available for this feature:

BLAST of Carg09228 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029612.1	2.0e-244	100.00	Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subs...	[more]
XP_023546001.1	6.5e-243	99.55	eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP...	[more]
KAG6598667.1	3.2e-242	99.55	Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subs...	[more]
XP_022962417.1	1.6e-241	99.33	probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_0...	[more]
XP_022997142.1	4.4e-239	98.44	probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022...	[more]

BLAST of Carg09228 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48724	2.1e-159	68.94	Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5...	[more]
Q9C8F1	8.2e-156	66.96	Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX...	[more]
P55876	1.4e-155	67.18	Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1	[more]
Q9S825	2.7e-151	65.85	Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX...	[more]
P59325	2.2e-47	32.89	Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1...	[more]

BLAST of Carg09228 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HCN3	7.8e-242	99.33	probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1K464	2.1e-239	98.44	probable eukaryotic translation initiation factor 5-1 OS=Cucurbita maxima OX=366...	[more]
A0A6J1BQ30	1.0e-217	88.64	eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 G...	[more]
A0A0A0LMB4	2.5e-216	89.53	W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=...	[more]
A0A1S3BCI3	8.1e-215	89.09	eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg09228 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36730.1	5.9e-157	66.96	Translation initiation factor IF2/IF5	[more]
AT1G77840.1	1.9e-152	65.85	Translation initiation factor IF2/IF5	[more]
AT5G20920.1	5.9e-08	30.11	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.2	5.9e-08	30.11	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.3	5.9e-08	30.11	eukaryotic translation initiation factor 2 beta subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 444..449
None	No IPR available	GENE3D	3.30.30.170		coord: 2..104 e-value: 2.0E-42 score: 145.9
None	No IPR available	GENE3D	2.20.25.350		coord: 105..145 e-value: 2.8E-19 score: 70.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..225
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 143..238
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 268..295
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 148..190
None	No IPR available	PANTHER	PTHR23001:SF28	EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATED	coord: 1..449
None	No IPR available	PANTHER	PTHR23001	EUKARYOTIC TRANSLATION INITIATION FACTOR	coord: 1..449
None	No IPR available	CDD	cd11561	W2_eIF5	coord: 296..447 e-value: 2.27137E-36 score: 129.273
IPR002735	Translation initiation factor IF2/IF5	SMART	SM00653	eIF2Bneu4	coord: 15..129 e-value: 3.5E-63 score: 225.9
IPR002735	Translation initiation factor IF2/IF5	PFAM	PF01873	eIF-5_eIF-2B	coord: 11..127 e-value: 2.2E-36 score: 124.3
IPR003307	W2 domain	SMART	SM00515	542_3	coord: 357..445 e-value: 9.9E-28 score: 108.1
IPR003307	W2 domain	PFAM	PF02020	W2	coord: 371..449 e-value: 5.9E-19 score: 68.0
IPR003307	W2 domain	PROSITE	PS51363	W2	coord: 276..449 score: 23.838604
IPR016021	MIF4G-like domain superfamily	GENE3D	1.25.40.180		coord: 295..449 e-value: 3.5E-26 score: 94.0
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 300..443
IPR016190	Translation initiation factor IF2/IF5, zinc-binding	SUPERFAMILY	75689	Zinc-binding domain of translation initiation factor 2 beta	coord: 98..133
IPR016189	Translation initiation factor IF2/IF5, N-terminal	SUPERFAMILY	100966	Translation initiation factor 2 beta, aIF2beta, N-terminal domain	coord: 10..101

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09228-RA	Carg09228-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001732	formation of cytoplasmic translation initiation complex
biological_process	GO:0001731	formation of translation preinitiation complex
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005829	cytosol
molecular_function	GO:0071074	eukaryotic initiation factor eIF2 binding
molecular_function	GO:0005092	GDP-dissociation inhibitor activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0005515	protein binding