Carg09028 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09028
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionreplication protein A 32 kDa subunit B-like
LocationCarg_Chr11: 1978459 .. 1983181 (-)
RNA-Seq ExpressionCarg09028
SyntenyCarg09028
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGACGAGAAAGATGATGGCGAATTTGTGCTGTCTTTGGATAATCTTCCATCCAGATGCTCGTCTTTGGGTTGCTCCTCTCGTTTGTACTACTACAGGCGAACCACTGAAGGGGTTCCCTTTCAGTGGGAGATGCAACCGGGGACCCCCAAGGATCCGCCATACACGGAGGCTGTTACGCTGCTGATCACTCCACCGCCGGCTGCGCTCAGCCTACGCCCGCCGAAGCCAGCAAGGACTCAGGGTAAACAATCGTTGTTTCTGTTGTTTAGGCCGTGGAGGAAGCAGTCCAAGTCCCAGAAGCATTCTTGTCTGTCGGATGAGTTTGAGGATGGACGGACGAGATTTGGGTCTTGTGGGTCCCAATGTGAGTTTATCCAATCACTATGCGAGCCAAAATCTTCTTCTTCTTCTTCTTCTTGGTCGTCGTTGCCGCCGGCGGAATCTTCCGCTCACCGGCGGTTGGGATGCGGTCCGTGGAGGATCAACCCACTAAGAATTGCTATTGGAAGGCGAGTTTGATTTCAAATATTCTCTCCTTTCGTTATTGCTTAGTAGATTTTGTAAATAAATACATAAATAAATAAATGTTTTTTTTTTTTTGTCAATTTTATAGATTATCCGTAATCCATTGTTGAATTTAAAAAATCTTAATTTTTTAAAATTTCATTTGAGTGATTCTTTTGACTTGAATTTTAGAGATAAATAAAAGAATAAATTTGAAATAGTGAAAAAAAATCCATGCATTTTTTAAACTAAATAAAAAACTCACAAACTAATAAATAAAACCGGGCAAAATTCAATCAATAATGCATATTGTCTACTTTAAACATAAGCTCTCGTTACTTTGGTTTGAGCTTCCCAATATGGCTTTATACTAATAAAGAGAGTATTATTTAATTACAAACTAATCATTTCTTAAATTAATCGATGTGGGATTTTCATCTTAGCCAACTACATTATAGGTAAATTTTTAAAGAGGGAGAGAGAGTGCGGAAACAAGCATTATATATATATATATATACAAAAGATAAATTTTTAAAGAGGGAGATAGAAAGCGCGCGGAAACAAGCATTATATGTGTGTGTATATATATATATCTATAAGGTAAATTTTTAAAGAGGGAGAGAGAGAGCGCGCGGAAACAAGCATAAGGGCAAAAAAGTAATAGAATTCTGTTATCCGAAAATTGAATAAAATAAGGCTCTGAGAAATGGCGGGAGGGAAGCTGAACCGGTACTTCTAGGTTTTAACAAAATGAAAATTTAATTTCGAAAATGCTCTTCAATTTGCTCTCGGTCAGTCACTTGATGCTCTTGTAGAGTTTGAAGATTCTTCGCTTGAAGCATTACAGTAGAAGAATGATGTATGGAAGTCAATTCGACGGCAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGGCTACCCAGGCCCCTGATCCTTCCTTCTCGCCGGCGAAAGTACGTTCTATCTCTAGATATAATATTAACTCTCTTTTGGATTCCTTTCATTTCTGTATGGATTACTAGTCTCCGCTGTTCGGATTTATAACATGCGTATTAACGTATTTTTGCTACCTGAAATCGTTCCAGCAGTTTGAAATGATTCGTACTTTTATTACTAATTAGGGGCACAATAATTCGTATATCTATACGATTTGACGACTGGTTCATATAATTGAAGTCTGGTTGGTCTAACGGTATTTTTGCCTCTACGTTTTCCGCGGCAATAGTTTATTCTCCTATCAGTTGTCTGGAAAATTAAGTTGAATTTGTTTCTCTTCGTTTATTGGAATTGGATACGCATGTTTTCCTTTCTTATCTCCTTCAGAAAGTATATTATCTATTAAATGAAGTCTTATATTTTTTAGTCTAATTTGCAGAATCGAGATGTCCAGTCATTGCTTCCGTTAACAGTGAAGCAGATAAACGACGCATTCTTGTCTAGTGGTGATCAATCGAATTTTGTTATTGATGGCGTTGATGTTAATAATGTTAGTGATTGAACTTGTATGTTTTGTTTATTTTTATTCGTTGAGTTGCTTGTTATGAGCTGCAATATGTTCAGGTTAAGCTTGTAGGAATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACCTTTTCACTGGATGATGGCACTGGATGGATTGATTGTAACAAATGGTTTGTTATGCTGCTCCCAATATGATGGAAAAGAAGTTAAGATGTTTCCCGTGTTGATATCGATTTTGTTTGTTGTATCTAATAGGGTCAATGAAGCTGCAGATTCAAGTGAAGTTGAGGGAATACTGTAATGTTCTTTTTACCTTGTCCTAATAACATTAAAAAAATTCCCTCAAAATCTTGTAATTTAAAATTCTCATGATTGGTTCTTCACTGTTGCCTAGAATCTGTTGTTATTTAGGATGGAACTTGACATATTGTAGACATGGAGTTCGAAAGGAATTTTGTGGTTCTTAAGTATTCGTTCAAGGAATTCTCTTACTAATTTTTAGTACAATGAAATATATTGACCGAGCGCCAAGGAAAATTTGCAAATTTCACACACAAATATTAGAATTCAAGTAAGCTTAAATCTAGTTAAATATGATTCAATATAGATATAGTACGCCCTCAGTTTGTAGCCTGCATAGATCTTTATGAGCATGCCTGTGAGGTTATGACCAAACCAGTAGTCTTATTCGTATATATTTGCAATTCATTGTTCTGTCTTATCCTACGGGAGTTTGTATATTTTCTTTTTCTAGCCAATGGATGACTAAGTTTGACCTGAAATTAAATCTTGAGAGTTTTGATGATTTGGGGGATGGGTCTGCTTATTTTGGACTAAGTGATGTATTCAGTCATCTCTCTGAAATTTATTTCCTTTTAGCGAATCTTTTTTTAACTAAGATTCTAATGCTTCAGGGATGGCATGTACGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGAAGAACTTTGAATGTCTTTTCTATCAGGTATAACATAGTTTTTCCATAGTTCTTTTTTTGGGGTCTCTTGGTTTTTTTTTTTTATTTCAGAGGGATTTGGTTTGTGAGTCAAACTGTATTAAAACAGTTCTTGAACTGTAGTGTATATGAGTTTATGGTCATAATTTTAGCACTTGAACTCAGATGAGACGCATTAATTATGATTTTGCACCTGTTGAAATTTTGATATTTTTGTAGCTTAATCATTGCATGAGCAGTTCACCACATGCAACTTGTGTACTTGATTTCCATAATATTGCAGTGGGGGATTTTGATGTAAAATATTTGTTTTCCAGGCCTGTGACCGACTATAATGAGATCACAAGTCACTTCATTGAATGCATATATGTTCATTTTTACAACACCAGGTTACGGGTATCAATCGAGCCATTTCAGATTTTAGTTGATTTTTTTACTAGGCGAAAAGTAATATTCATTGTCTTATCTGCTTCTCCTTTTGGGTTATGTTCATATTATGCTGATAGAAACAACAAAGTAGCAGTATGACTACTCAGCCACCGGTGCCAAATTTATCTAATACTCCTAAGAAAGGATATCAAGCTCCCATTCTTAATCAAGTAAGATTGTAGTTCAATCCACATGTGGTGAGCTACAGTTTGTTTATGCATCCTTCTGATGATGCTTTTTCAGTATTCTGGTCAAGTGGGTGGTGATGGTTGGAAGAGCCTTGAGCAGAAGATTTTAGATTTTTTGCAACTTCCTTCATGCGAGTGAGTGCCTTTTAACAATGCTGCATATATTTTCACTGCATATATTTTTTCTCCTTTTCATGATAGATAACTGGATTCTTGAGGAGATACTCGACCTCCATAGCTTGATGTTTATTATTACTTATTAGGATCCTCAAATAACTCAAGCACTCTTGTATGCCTTACCAATCATTCAAGCAGTCATAGAAAAGGTTGTCCCTAGATGAGACTTATTGAGAAGAGTTGTGGTCCTAATCTTACTTAATTGGTATTTCCTTTAAGAGCATCTAATTAAGTATAGAATGGTACGACATTAAAAATATTTGTCTCGATATAAGCAACAATCTATAGTGTTACTTGTACTATGTTGAATGATGAATAAGGTTTGTTCGAGTGTCATGATTGATCCTGAATATTTTGAGCGAGGGCAGTAATAATTAGGGCTCGTTGTAGTTTCTTTATTCAATTTCTTATGGGTTTGTGTTATGGTTTCTTTTCTTTTTGAATGGAACGTCATTTGTCAAGCAGATTACATCATATAGGGATGACCTGTTCATCTTTCCTTGGTATAATTGCCTTGGTCTATGTTTCTCTCTTTCTGAAATTGATTAGTGTTATGTTTTTTTATTCTCCTTTTATTTTTTTTAGTGAAAGACAAAGTCTGGAATGCTCCCTCACTATTTTCATTCCCTGCTCATCTGTTTCTTAGTTTTAATTTATTATTGGGATCTATATATTCACTCTTCCTATGTTCAGTACTGAGAGGGGTGCACACCGTGATGTCATTGCTCAACATCTTAAAGTTCCACTCGAGAAGCTCATGTATTACACTGTTCCTCACACTTCTACTTGACGGCAGCACTTATCCTTCAAATAGGGGTTAACTAAGCATACCATTTTGCTTATTATTTTGCAGTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTGATGTACTCAACTACTGATGAATATCACTTCAAATCAACTGCCAACGGTTGACGTCTCT

mRNA sequence

ATGAAGGACGAGAAAGATGATGGCGAATTTGTGCTGTCTTTGGATAATCTTCCATCCAGATGCTCGTCTTTGGGTTGCTCCTCTCGTTTGTACTACTACAGGCGAACCACTGAAGGGGTTCCCTTTCAGTGGGAGATGCAACCGGGGACCCCCAAGGATCCGCCATACACGGAGGCTGTTACGCTGCTGATCACTCCACCGCCGGCTGCGCTCAGCCTACGCCCGCCGAAGCCAGCAAGGACTCAGGGTAAACAATCGTTGTTTCTGTTGTTTAGGCCGTGGAGGAAGCAGTCCAAGTCCCAGAAGCATTCTTGTCTGTCGGATGAGTTTGAGGATGGACGGACGAGATTTGGGTCTTGTGGGTCCCAATGTGAGTTTATCCAATCACTATGCGAGCCAAAATCTTCTTCTTCTTCTTCTTCTTGGTCGTCGTTGCCGCCGGCGGAATCTTCCGCTCACCGGCGGTTGGGATGCGGTCCGTGGAGGATCAACCCACTAAGAATTGCTATTGGAAGGCGAAGTTTGAAGATTCTTCGCTTGAAGCATTACAGTAGAAGAATGATGTATGGAAGTCAATTCGACGGCAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGGCTACCCAGGCCCCTGATCCTTCCTTCTCGCCGGCGAAAAATCGAGATGTCCAGTCATTGCTTCCGTTAACAGTGAAGCAGATAAACGACGCATTCTTGTCTAGTGGTGATCAATCGAATTTTGTTATTGATGGCGTTGATGTTAATAATGTTAAGCTTGTAGGAATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACCTTTTCACTGGATGATGGCACTGGATGGATTGATTGTAACAAATGGGTCAATGAAGCTGCAGATTCAAGTGAAGTTGAGGGAATACTGGATGGCATGTACGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGAAGAACTTTGAATGTCTTTTCTATCAGGCCTGTGACCGACTATAATGAGATCACAAGTCACTTCATTGAATGCATATATGTTCATTTTTACAACACCAGGTTACGGAAACAACAAAGTAGCAGTATGACTACTCAGCCACCGGTGCCAAATTTATCTAATACTCCTAAGAAAGGATATCAAGCTCCCATTCTTAATCAATATTCTGGTCAAGTGGGTGGTGATGGTTGGAAGAGCCTTGAGCAGAAGATTTTAGATTTTTTGCAACTTCCTTCATGCGATACTGAGAGGGGTGCACACCGTGATGTCATTGCTCAACATCTTAAAGTTCCACTCGAGAAGCTCATTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTGATGTACTCAACTACTGATGAATATCACTTCAAATCAACTGCCAACGGTTGACGTCTCT

Coding sequence (CDS)

ATGAAGGACGAGAAAGATGATGGCGAATTTGTGCTGTCTTTGGATAATCTTCCATCCAGATGCTCGTCTTTGGGTTGCTCCTCTCGTTTGTACTACTACAGGCGAACCACTGAAGGGGTTCCCTTTCAGTGGGAGATGCAACCGGGGACCCCCAAGGATCCGCCATACACGGAGGCTGTTACGCTGCTGATCACTCCACCGCCGGCTGCGCTCAGCCTACGCCCGCCGAAGCCAGCAAGGACTCAGGGTAAACAATCGTTGTTTCTGTTGTTTAGGCCGTGGAGGAAGCAGTCCAAGTCCCAGAAGCATTCTTGTCTGTCGGATGAGTTTGAGGATGGACGGACGAGATTTGGGTCTTGTGGGTCCCAATGTGAGTTTATCCAATCACTATGCGAGCCAAAATCTTCTTCTTCTTCTTCTTCTTGGTCGTCGTTGCCGCCGGCGGAATCTTCCGCTCACCGGCGGTTGGGATGCGGTCCGTGGAGGATCAACCCACTAAGAATTGCTATTGGAAGGCGAAGTTTGAAGATTCTTCGCTTGAAGCATTACAGTAGAAGAATGATGTATGGAAGTCAATTCGACGGCAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGGCTACCCAGGCCCCTGATCCTTCCTTCTCGCCGGCGAAAAATCGAGATGTCCAGTCATTGCTTCCGTTAACAGTGAAGCAGATAAACGACGCATTCTTGTCTAGTGGTGATCAATCGAATTTTGTTATTGATGGCGTTGATGTTAATAATGTTAAGCTTGTAGGAATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACCTTTTCACTGGATGATGGCACTGGATGGATTGATTGTAACAAATGGGTCAATGAAGCTGCAGATTCAAGTGAAGTTGAGGGAATACTGGATGGCATGTACGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGAAGAACTTTGAATGTCTTTTCTATCAGGCCTGTGACCGACTATAATGAGATCACAAGTCACTTCATTGAATGCATATATGTTCATTTTTACAACACCAGGTTACGGAAACAACAAAGTAGCAGTATGACTACTCAGCCACCGGTGCCAAATTTATCTAATACTCCTAAGAAAGGATATCAAGCTCCCATTCTTAATCAATATTCTGGTCAAGTGGGTGGTGATGGTTGGAAGAGCCTTGAGCAGAAGATTTTAGATTTTTTGCAACTTCCTTCATGCGATACTGAGAGGGGTGCACACCGTGATGTCATTGCTCAACATCTTAAAGTTCCACTCGAGAAGCTCATTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTGATGTACTCAACTACTGATGAATATCACTTCAAATCAACTGCCAACGGTTGA

Protein sequence

MKDEKDDGEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKDPPYTEAVTLLITPPPAALSLRPPKPARTQGKQSLFLLFRPWRKQSKSQKHSCLSDEFEDGRTRFGSCGSQCEFIQSLCEPKSSSSSSSWSSLPPAESSAHRRLGCGPWRINPLRIAIGRRSLKILRLKHYSRRMMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDGMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Homology
BLAST of Carg09028 vs. NCBI nr
Match: KAG7021722.1 (Replication protein A 32 kDa subunit B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 944.5 bits (2440), Expect = 3.4e-271
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MKDEKDDGEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKDPPYTEAV 60
           MKDEKDDGEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKDPPYTEAV
Sbjct: 1   MKDEKDDGEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKDPPYTEAV 60

Query: 61  TLLITPPPAALSLRPPKPARTQGKQSLFLLFRPWRKQSKSQKHSCLSDEFEDGRTRFGSC 120
           TLLITPPPAALSLRPPKPARTQGKQSLFLLFRPWRKQSKSQKHSCLSDEFEDGRTRFGSC
Sbjct: 61  TLLITPPPAALSLRPPKPARTQGKQSLFLLFRPWRKQSKSQKHSCLSDEFEDGRTRFGSC 120

Query: 121 GSQCEFIQSLCEPKSSSSSSSWSSLPPAESSAHRRLGCGPWRINPLRIAIGRRSLKILRL 180
           GSQCEFIQSLCEPKSSSSSSSWSSLPPAESSAHRRLGCGPWRINPLRIAIGRRSLKILRL
Sbjct: 121 GSQCEFIQSLCEPKSSSSSSSWSSLPPAESSAHRRLGCGPWRINPLRIAIGRRSLKILRL 180

Query: 181 KHYSRRMMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAF 240
           KHYSRRMMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAF
Sbjct: 181 KHYSRRMMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAF 240

Query: 241 LSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSE 300
           LSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSE
Sbjct: 241 LSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSE 300

Query: 301 VEGILDGMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQS 360
           VEGILDGMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQS
Sbjct: 301 VEGILDGMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQS 360

Query: 361 SSMTTQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAH 420
           SSMTTQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAH
Sbjct: 361 SSMTTQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAH 420

Query: 421 RDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           RDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Sbjct: 421 RDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 463

BLAST of Carg09028 vs. NCBI nr
Match: XP_022930148.1 (replication protein A 32 kDa subunit B-like [Cucurbita moschata] >XP_022930155.1 replication protein A 32 kDa subunit B-like [Cucurbita moschata] >XP_022930161.1 replication protein A 32 kDa subunit B-like [Cucurbita moschata])

HSP 1 Score: 568.2 bits (1463), Expect = 6.7e-158
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 277

BLAST of Carg09028 vs. NCBI nr
Match: KAG6587750.1 (Replication protein A 32 kDa subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-156
Identity = 275/277 (99.28%), Postives = 276/277 (99.64%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKLVGMVRNRAGRIT VTFSLDDGTGWIDCNKWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLVGMVRNRAGRITAVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTPKKGYQAPILNQYSG+VGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPKKGYQAPILNQYSGRVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 277

BLAST of Carg09028 vs. NCBI nr
Match: XP_023530472.1 (replication protein A 32 kDa subunit B-like [Cucurbita pepo subsp. pepo] >XP_023530473.1 replication protein A 32 kDa subunit B-like [Cucurbita pepo subsp. pepo] >XP_023530475.1 replication protein A 32 kDa subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-155
Identity = 273/277 (98.56%), Postives = 276/277 (99.64%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MM+GSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMHGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDC+KWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCSKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTP KGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDT+RGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPMKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTDRGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 277

BLAST of Carg09028 vs. NCBI nr
Match: XP_023007037.1 (replication protein A 32 kDa subunit B-like [Cucurbita maxima] >XP_023007045.1 replication protein A 32 kDa subunit B-like [Cucurbita maxima] >XP_023007055.1 replication protein A 32 kDa subunit B-like [Cucurbita maxima])

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-154
Identity = 272/277 (98.19%), Postives = 274/277 (98.92%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKL+GMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLIGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIR VTDYNEITSHFIECIYVHFYNTRL+KQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRLVTDYNEITSHFIECIYVHFYNTRLQKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTP KGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPMKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYH KSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHVKSTANG 277

BLAST of Carg09028 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.2e-70
Identity = 140/280 (50.00%), Postives = 187/280 (66.79%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQS 247
           MYG  FDGNAAF+GGGFMPSQAT     S S  KNRDV++LLPLT+KQ++ A  S+  +S
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSA--STTGES 60

Query: 248 NFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDG 307
           NF IDGVD+  V +VG +     RIT V F +DDGTGW+DC +W +   ++ E+E +  G
Sbjct: 61  NFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLG 120

Query: 308 MYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLR--KQQSSSMT 367
           MYVR+HGHLK FQG R++NVFS+RPVTD+NEI  HF EC+YVH YNT+LR       + T
Sbjct: 121 MYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTAT 180

Query: 368 TQPPVP-NLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLP-SCDTERGAHRD 427
            +P +P +   TP K YQ    NQ+  Q   D    ++Q +L++L  P    +E G H D
Sbjct: 181 PRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 240

Query: 428 VIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTAN 463
           +IA+ L++PL ++  A++ L  +G +YST DE  FKSTAN
Sbjct: 241 IIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 277

BLAST of Carg09028 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 256.9 bits (655), Expect = 4.3e-67
Identity = 133/277 (48.01%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI + F SSG++
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNR-AGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGIL 306
           S  VI+G+ + NV LVG+V ++   ++T+V F+LDDGTG IDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 307 DGMYVRVHGHLKSFQGRT-LNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMT 366
           DG YVR+ GHLK+FQG+T L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ   +T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 367 --TQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDT-ERGAHR 426
                     SNT +     P+++  +    G+G K+L+  ILD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 427 DVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFK 459
           D IAQ LK+P  KL   +++LE +GL+YST DEYHFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Carg09028 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.2e-48
Identity = 107/288 (37.15%), Postives = 173/288 (60.07%), Query Frame = 0

Query: 190 GSQFDG-----NAAFSGGGFMPSQATQAPDPSFSPA----KNRDVQSLLPLTVKQINDAF 249
           G  +DG     ++ F GGGFMPSQAT A + +        K+R+ Q+LLPLTVKQI DA 
Sbjct: 11  GGNYDGGGGNASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDAS 70

Query: 250 LSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSE 309
            ++ D+SNF ++G++V+ V+LVG + N+  R+TDV+F+LDDGTG +  N+W N++ D+ E
Sbjct: 71  QTNDDKSNFAVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKE 130

Query: 310 VEGILDGMYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQ 369
           +  I +G YV V+G LK FQG R +  +S+R +T++N++T HF+ C++VH   TR + Q 
Sbjct: 131 MADIQNGDYVIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQV 190

Query: 370 SSSMTTQPPVPNLSNTPKKGYQAPILNQYS----GQVGGDGWKSLEQKILDFLQLPSCDT 429
           +++  T  P   +        Q+P+ NQ S     Q  G G   ++  +  F      + 
Sbjct: 191 NANTATGTPNQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMND 250

Query: 430 ERGAHRDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           + G   D +++ L +P E +   + +  + G +Y+T D++H+KST NG
Sbjct: 251 DHGVGVDYVSRRLNLPEETVGKIIIDQVDLGHLYATIDDHHYKSTMNG 298

BLAST of Carg09028 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.4e-43
Identity = 116/290 (40.00%), Postives = 167/290 (57.59%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLS--SG 246
           MM  SQ D   AFS   F  SQ   A   S +P+K+R   S +PLTVKQI++A  S  +G
Sbjct: 1   MMSFSQPD---AFSPSQFTSSQ--NAAADSTTPSKSRGASSTMPLTVKQISEAQQSGITG 60

Query: 247 DQ-SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEG 306
           ++ + FV+DGV+  NV+LVG+V  +  R TDV+F++DDGTG +D  +WVN+ ADS+E   
Sbjct: 61  EKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAA 120

Query: 307 ILDGMYVRVHGHLKSFQGR-TLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSS 366
           + +GMYV V G LK  Q R     F+IRPVTDYNE+T HFI+C+ +H  NT  + Q  S 
Sbjct: 121 VQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENT--KSQIGSP 180

Query: 367 MTTQPPVPNLSN---------TPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLP-S 426
             T   + + S+         T  K   AP+L+  +G         L  ++L+  + P +
Sbjct: 181 AKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSK-----TDLNTEVLNVFREPAN 240

Query: 427 CDTERGAHRDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTAN 463
            ++E G H D I +  ++P  K+  A+  L + G +YST DE H+KS  N
Sbjct: 241 VESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSAFN 278

BLAST of Carg09028 vs. ExPASy Swiss-Prot
Match: Q92373 (Replication factor A protein 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ssb2 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.6e-24
Identity = 92/286 (32.17%), Postives = 142/286 (49.65%), Query Frame = 0

Query: 189 YGSQFDGNAAFS---GGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGD 248
           YG  F  N+AFS   GGG   ++  Q+  PS    +      L P+T+KQI +A     D
Sbjct: 11  YGPDF--NSAFSPGMGGGAGFNEYDQSSQPSVDRQQGAG-NKLRPVTIKQILNASQVHAD 70

Query: 249 QSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGIL 308
            + F IDGV+V  V  VG++RN   + T+ T+ ++DGTG I+   W +  A S     + 
Sbjct: 71  -AEFKIDGVEVGQVTFVGVLRNIHAQTTNTTYQIEDGTGMIEVRHWEHIDALSE----LA 130

Query: 309 DGMYVRVHGHLKSFQGRTLNVFS-IRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMT 368
              YVRV+G++K F G+       IR + D+NE+  HF+E I VH + T    Q+++++ 
Sbjct: 131 TDTYVRVYGNIKIFSGKIYIASQYIRTIKDHNEVHFHFLEAIAVHLHFT----QKANAV- 190

Query: 369 TQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLP-----------SC 428
                 N +N P  GY       Y+         SLEQK+ ++   P           + 
Sbjct: 191 ------NGANAP--GYGTSNALGYNNISSNGAANSLEQKLAEYSLTPAQMTVMQAIHSAP 250

Query: 429 DTERGAHRDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKS 460
           +T  G H   +AQ +   ++ L      L++EG++Y+T DE HFKS
Sbjct: 251 ETNEGVHVRQLAQSVGPGID-LTAVTDFLQQEGIIYTTIDENHFKS 274

BLAST of Carg09028 vs. ExPASy TrEMBL
Match: A0A6J1EU79 (replication protein A 32 kDa subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111436658 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 3.2e-158
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 277

BLAST of Carg09028 vs. ExPASy TrEMBL
Match: A0A6J1L1W1 (replication protein A 32 kDa subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111499646 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 5.7e-155
Identity = 272/277 (98.19%), Postives = 274/277 (98.92%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ
Sbjct: 1   MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 306
           SNFVIDGVDVNNVKL+GMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD
Sbjct: 61  SNFVIDGVDVNNVKLIGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILD 120

Query: 307 GMYVRVHGHLKSFQGRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 366
           GMYVRVHGHLKSFQGRTLNVFSIR VTDYNEITSHFIECIYVHFYNTRL+KQQSSSMTTQ
Sbjct: 121 GMYVRVHGHLKSFQGRTLNVFSIRLVTDYNEITSHFIECIYVHFYNTRLQKQQSSSMTTQ 180

Query: 367 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 426
           PPVPNLSNTP KGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PPVPNLSNTPMKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 240

Query: 427 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKLIPAMKNLEEEGLMYSTTDEYH KSTANG
Sbjct: 241 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHVKSTANG 277

BLAST of Carg09028 vs. ExPASy TrEMBL
Match: A0A1S4DZE3 (replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494262 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 6.8e-140
Identity = 250/277 (90.25%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQS 247
           MY SQFDGNAAFSGGGFMPSQ TQAPD SFSPAKNRDVQ+LLPLTVKQINDAFLSS D+S
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 248 NFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDG 307
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTF+LDDGTG IDC+KWVNEAADS+EVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 308 MYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 367
           MYVRVHGHLKSFQG RTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 368 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 427
           P + NLSNTP KGYQAPI NQY+GQ GGD WKSLEQ +LDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 428 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
            LKVPLEKLIPAMKNLEEEGL+YSTTD+YHFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 277

BLAST of Carg09028 vs. ExPASy TrEMBL
Match: A0A5D3CIS3 (Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00360 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 6.8e-140
Identity = 250/277 (90.25%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQS 247
           MY SQFDGNAAFSGGGFMPSQ TQAPD SFSPAKNRDVQ+LLPLTVKQINDAFLSS D+S
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 248 NFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDG 307
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTF+LDDGTG IDC+KWVNEAADS+EVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 308 MYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 367
           MYVRVHGHLKSFQG RTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 368 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 427
           P + NLSNTP KGYQAPI NQY+GQ GGD WKSLEQ +LDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 428 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
            LKVPLEKLIPAMKNLEEEGL+YSTTD+YHFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 277

BLAST of Carg09028 vs. ExPASy TrEMBL
Match: A0A6J1JJ51 (replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC111485578 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 5.7e-139
Identity = 246/277 (88.81%), Postives = 261/277 (94.22%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQS 247
           MYGSQFDGNAAFSGGGFMPSQ T APD S SPAKNRDVQ+LLPLTVKQINDAFLSS D+S
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 248 NFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDG 307
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTF+LDDGTG IDC+KWVNEAADS+EVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 308 MYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMTTQ 367
           MYVRVHGHLKSFQG RTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 368 PPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDTERGAHRDVIAQ 427
           P + NLSNTP +GYQ P+ NQY+GQ GGDGWK+LEQ +LDFLQLPSCDTERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPMSNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 428 HLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTANG 464
           HLKVPLEKL+PAMKNLEEEGL+YSTTD+YHFKSTANG
Sbjct: 241 HLKVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 277

BLAST of Carg09028 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-71
Identity = 140/280 (50.00%), Postives = 187/280 (66.79%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQS 247
           MYG  FDGNAAF+GGGFMPSQAT     S S  KNRDV++LLPLT+KQ++ A  S+  +S
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSA--STTGES 60

Query: 248 NFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGILDG 307
           NF IDGVD+  V +VG +     RIT V F +DDGTGW+DC +W +   ++ E+E +  G
Sbjct: 61  NFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLG 120

Query: 308 MYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLR--KQQSSSMT 367
           MYVR+HGHLK FQG R++NVFS+RPVTD+NEI  HF EC+YVH YNT+LR       + T
Sbjct: 121 MYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTAT 180

Query: 368 TQPPVP-NLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLP-SCDTERGAHRD 427
            +P +P +   TP K YQ    NQ+  Q   D    ++Q +L++L  P    +E G H D
Sbjct: 181 PRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 240

Query: 428 VIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTAN 463
           +IA+ L++PL ++  A++ L  +G +YST DE  FKSTAN
Sbjct: 241 IIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 277

BLAST of Carg09028 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 256.9 bits (655), Expect = 3.1e-68
Identity = 133/277 (48.01%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI + F SSG++
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNR-AGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGIL 306
           S  VI+G+ + NV LVG+V ++   ++T+V F+LDDGTG IDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 307 DGMYVRVHGHLKSFQGRT-LNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMT 366
           DG YVR+ GHLK+FQG+T L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ   +T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 367 --TQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDT-ERGAHR 426
                     SNT +     P+++  +    G+G K+L+  ILD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 427 DVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFK 459
           D IAQ LK+P  KL   +++LE +GL+YST DEYHFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Carg09028 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 256.9 bits (655), Expect = 3.1e-68
Identity = 133/277 (48.01%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 187 MMYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAKNRDVQSLLPLTVKQINDAFLSSGDQ 246
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI + F SSG++
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 247 SNFVIDGVDVNNVKLVGMVRNR-AGRITDVTFSLDDGTGWIDCNKWVNEAADSSEVEGIL 306
           S  VI+G+ + NV LVG+V ++   ++T+V F+LDDGTG IDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 307 DGMYVRVHGHLKSFQGRT-LNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSMT 366
           DG YVR+ GHLK+FQG+T L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ   +T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 367 --TQPPVPNLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQKILDFLQLPSCDT-ERGAHR 426
                     SNT +     P+++  +    G+G K+L+  ILD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 427 DVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFK 459
           D IAQ LK+P  KL   +++LE +GL+YST DEYHFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Carg09028 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-67
Identity = 140/300 (46.67%), Postives = 187/300 (62.33%), Query Frame = 0

Query: 188 MYGSQFDGNAAFSGGGFMPSQATQAPDPSFSPAK--------------------NRDVQS 247
           MYG  FDGNAAF+GGGFMPSQAT     S S  K                    NRDV++
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKVYVSDSRRFSGISAAKSSIRNRDVRT 60

Query: 248 LLPLTVKQINDAFLSSGDQSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFSLDDGTGWID 307
           LLPLT+KQ++ A  S+  +SNF IDGVD+  V +VG +     RIT V F +DDGTGW+D
Sbjct: 61  LLPLTLKQLSSA--STTGESNFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVD 120

Query: 308 CNKWVNEAADSSEVEGILDGMYVRVHGHLKSFQG-RTLNVFSIRPVTDYNEITSHFIECI 367
           C +W +   ++ E+E +  GMYVR+HGHLK FQG R++NVFS+RPVTD+NEI  HF EC+
Sbjct: 121 CVRWCHARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECM 180

Query: 368 YVHFYNTRLR--KQQSSSMTTQPPVP-NLSNTPKKGYQAPILNQYSGQVGGDGWKSLEQK 427
           YVH YNT+LR       + T +P +P +   TP K YQ    NQ+  Q   D    ++Q 
Sbjct: 181 YVHMYNTKLRGGSITQDTATPRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQT 240

Query: 428 ILDFLQLP-SCDTERGAHRDVIAQHLKVPLEKLIPAMKNLEEEGLMYSTTDEYHFKSTAN 463
           +L++L  P    +E G H D+IA+ L++PL ++  A++ L  +G +YST DE  FKSTAN
Sbjct: 241 VLNYLNQPMHIVSEAGVHCDIIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 297

BLAST of Carg09028 vs. TAIR 10
Match: AT4G25845.1 (BEST Arabidopsis thaliana protein match is: OSBP(oxysterol binding protein)-related protein 4B (TAIR:AT4G25850.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-07
Identity = 38/85 (44.71%), Postives = 50/85 (58.82%), Query Frame = 0

Query: 19  SRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKDPPYTEAVTLLITPPPAALSLRPPKP 78
           SR SS+G     YY  R  EGVPF+WEMQPGTP +    E V  L +PPPA LSL  PKP
Sbjct: 13  SRNSSVG-----YYDERMPEGVPFEWEMQPGTPINTQPREVVPPL-SPPPAMLSLGLPKP 72

Query: 79  ARTQGKQSLFLLFRPWRKQSKSQKH 104
           + +  +   F+   P + + ++ KH
Sbjct: 73  SISIEEPKNFVF--PTKLKLRNWKH 89

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021722.13.4e-271100.00Replication protein A 32 kDa subunit B, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022930148.16.7e-158100.00replication protein A 32 kDa subunit B-like [Cucurbita moschata] >XP_022930155.1... [more]
KAG6587750.11.6e-15699.28Replication protein A 32 kDa subunit B, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023530472.11.1e-15598.56replication protein A 32 kDa subunit B-like [Cucurbita pepo subsp. pepo] >XP_023... [more]
XP_023007037.11.2e-15498.19replication protein A 32 kDa subunit B-like [Cucurbita maxima] >XP_023007045.1 r... [more]
Match NameE-valueIdentityDescription
Q8LFJ84.2e-7050.00Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ... [more]
Q9ZQ194.3e-6748.01Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ... [more]
Q6H7J51.2e-4837.15Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6K9U27.4e-4340.00Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q923731.6e-2432.17Replication factor A protein 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... [more]
Match NameE-valueIdentityDescription
A0A6J1EU793.2e-158100.00replication protein A 32 kDa subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1L1W15.7e-15598.19replication protein A 32 kDa subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A1S4DZE36.8e-14090.25replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A5D3CIS36.8e-14090.25Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1JJ515.7e-13988.81replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC1114855... [more]
Match NameE-valueIdentityDescription
AT3G02920.13.0e-7150.00Replication protein A, subunit RPA32 [more]
AT2G24490.13.1e-6848.01replicon protein A2 [more]
AT2G24490.23.1e-6848.01replicon protein A2 [more]
AT3G02920.21.2e-6746.67Replication protein A, subunit RPA32 [more]
AT4G25845.11.4e-0744.71BEST Arabidopsis thaliana protein match is: OSBP(oxysterol binding protein)-rela... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.140coord: 220..354
e-value: 4.3E-39
score: 135.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..148
NoneNo IPR availablePANTHERPTHR13989:SF42REPLICATION PROTEIN A 32 KDA SUBUNIT A-LIKEcoord: 190..463
NoneNo IPR availableCDDcd04478RPA2_DBD_Dcoord: 258..350
e-value: 4.22332E-40
score: 137.346
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 394..462
e-value: 1.2E-14
score: 55.7
IPR014892Replication protein A, C-terminalPFAMPF08784RPA_Ccoord: 349..454
e-value: 2.4E-12
score: 47.4
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 190..463
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 395..461
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 228..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09028-RACarg09028-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006260 DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006289 nucleotide-excision repair
cellular_component GO:0000781 chromosome, telomeric region
cellular_component GO:0005662 DNA replication factor A complex
cellular_component GO:0035861 site of double-strand break
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003697 single-stranded DNA binding