Carg08823 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08823
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glycine cleavage system H protein
Location	Carg_Chr02: 8993364 .. 8994902 (+)
RNA-Seq Expression	Carg08823
Synteny	Carg08823

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTAAAAATTAGAGGTCATTATGAGGCCATAACTCTTACAGATGAGGATATGGAAGGAAAAAGTGGGAATTTCACTGGAAGAAATAAGAAGGCAGTGTTTAGATGAGGCACTCACTGCCCACCATCCGTCGAAGATAAACCAACAATGTCGTAAAACTCAAGTTTTCAAAACTTCCTTTCATTTCTTTGCTCATTGCTGATAGCATACCAAAGCAGAGGAAGAACAAGAACAAGAAGAACAGACCCAAAATAAACAACAAAACTATGGCTCTCAGAATGTGGGTTTCTTCCACTGCTAATGCTCTCAGACTCTCTTCTTCATCCAAAACCCATCTCTCTCCTCCATTTTCTATCTCCAGATGCTTTTCTACTGGTATTTTTTTACTATTTCTTTCCTTGCCTGCAAGCTGTTTGTGTAAATGCCTCTGTGAACTTTGACTTTGTTTTTCTTTTTTTTTAATATACTGTTGAATCCCACTTCCATTGTTTGATGTTGTTTTATTCTCAATTATGTTTAATTTCTTATCTGGGTAACACAAAAGATGTTGGTTGGATTTGCAGTTTTGGATGGACTGAAGTATGCTTCTTCACATGAGTGGGTGAAACATGAGGGCCCTGTTGCTGCCATTGGCATCACTGACCATGCTCAGGTGCTTTTCATTTCTAAACGCCAACATTCTAACTTTTGTTTCCCTGAATTATAGATTAAACTCTAAGCTTGGTTCACGAAATCTTATTCTTATTCATACATTTTCATCATTGAATTCCTAATAAGTTACTGAAATTTCAAATTTGTGTGTAATGAGTTCATATCATTAACGTGGTTGAGTTATTGTCTAGATAGATTATAAAGGTTGAATTGAAAATCAACTACAGAGAACATGTCATGTTAGTGTTAAATGAAGTTAATGCAGTACCTTATTGAACACAAAATTGAAAGTGTTGAAACCTATTAAAAACTTTTATAATACAACTACGAAGGAGAAACAACACTGGTAAAAGTTTAGATACCAAACATATAATTTAACCTAAATTATGCGTGCATATGACAACTTACTCAAAACAATGGAAACTGATTCAAACTTTTATTTTAACATCTTGTATATCAGTCCACTATAGAAAATTTGGGTTTATACTTGAAATTCAAACTTGGGTTGGAAGCGTTTTGTTTGATGCATCTCTACAGGACCATCTGGGAGAAGTGGTGTTCGTGGATCTGCCAGAATCAGGAGCTCCGGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAGAGTGTAAAAGCAACCAGTGATATCAACTCCCCAATCTCTGGTGAGATTGTTGAAGTTAATCCACAGCTGAAGGATTCACCTGGTTTGGTAATATTTCAAATCCTCACATCTTTTTGCCTATTCTTTGGACTCCGAATTTGGCCGATAAACTGTTTTAAGTGCTTAATCTTGTTGGTGGATGAAAGTCCCACATCGGCCACGGTCCTACATTTTTTACCGCGCAGCGTTGTGATCTTGACATGCTCACGACCAGCCTTAAGGGGAATTAA

mRNA sequence

GCTAAAAATTAGAGGTCATTATGAGGCCATAACTCTTACAGATGAGGATATGGAAGGAAAAAGTGGGAATTTCACTGGAAGAAATAAGAAGGCAGTGTTTAGATGAGGCACTCACTGCCCACCATCCGTCGAAGATAAACCAACAATGTCGTAAAACTCAAGTTTTCAAAACTTCCTTTCATTTCTTTGCTCATTGCTGATAGCATACCAAAGCAGAGGAAGAACAAGAACAAGAAGAACAGACCCAAAATAAACAACAAAACTATGGCTCTCAGAATGTGGGTTTCTTCCACTGCTAATGCTCTCAGACTCTCTTCTTCATCCAAAACCCATCTCTCTCCTCCATTTTCTATCTCCAGATGCTTTTCTACTGTTTTGGATGGACTGAAGTATGCTTCTTCACATGAGTGGGTGAAACATGAGGGCCCTGTTGCTGCCATTGGCATCACTGACCATGCTCAGGACCATCTGGGAGAAGTGGTGTTCGTGGATCTGCCAGAATCAGGAGCTCCGGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAGAGTGTAAAAGCAACCAGTGATATCAACTCCCCAATCTCTGGTGAGATTGTTGAAGTTAATCCACAGCTGAAGGATTCACCTGGTTTGTCCCACATCGGCCACGGTCCTACATTTTTTACCGCGCAGCGTTGTGATCTTGACATGCTCACGACCAGCCTTAAGGGGAATTAA

Coding sequence (CDS)

ATGGCTCTCAGAATGTGGGTTTCTTCCACTGCTAATGCTCTCAGACTCTCTTCTTCATCCAAAACCCATCTCTCTCCTCCATTTTCTATCTCCAGATGCTTTTCTACTGTTTTGGATGGACTGAAGTATGCTTCTTCACATGAGTGGGTGAAACATGAGGGCCCTGTTGCTGCCATTGGCATCACTGACCATGCTCAGGACCATCTGGGAGAAGTGGTGTTCGTGGATCTGCCAGAATCAGGAGCTCCGGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAGAGTGTAAAAGCAACCAGTGATATCAACTCCCCAATCTCTGGTGAGATTGTTGAAGTTAATCCACAGCTGAAGGATTCACCTGGTTTGTCCCACATCGGCCACGGTCCTACATTTTTTACCGCGCAGCGTTGTGATCTTGACATGCTCACGACCAGCCTTAAGGGGAATTAA

Protein sequence

MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIGITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLSHIGHGPTFFTAQRCDLDMLTTSLKGN

Homology

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BLAST of Carg08823 vs. NCBI nr
Match: KAG7036177.1 (Glycine cleavage system H protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.1 bits (775), Expect = 1.3e-78
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG
Sbjct: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGLSHIGHGPTFFTAQRCDLDMLTTSLKGN 151
           PGLSHIGHGPTFFTAQRCDLDMLTTSLKGN
Sbjct: 121 PGLSHIGHGPTFFTAQRCDLDMLTTSLKGN 150

[Close]

BLAST of Carg08823 vs. NCBI nr
Match: XP_023532627.1 (glycine cleavage system H protein 3, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 241.1 bits (614), Expect = 6.1e-60
Identity = 121/123 (98.37%), Postives = 121/123 (98.37%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVA IG
Sbjct: 1   MALRMWASSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

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BLAST of Carg08823 vs. NCBI nr
Match: XP_022931257.1 (glycine cleavage system H protein, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 238.0 bits (606), Expect = 5.1e-59
Identity = 120/123 (97.56%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVA IG
Sbjct: 1   MALRMWASSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVN QLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNQQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. NCBI nr
Match: XP_022995342.1 (glycine cleavage system H protein 3, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-58
Identity = 120/123 (97.56%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSSSSKT LSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVA IG
Sbjct: 1   MALRMWASSTANALRLSSSSKTLLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. NCBI nr
Match: KAG6606235.1 (Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.5 bits (602), Expect = 1.5e-58
Identity = 118/119 (99.16%), Postives = 118/119 (99.16%), Query Frame = 0


Query: 5   MWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIGITDH 64
           MW SSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIGITDH
Sbjct: 1   MWASSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIGITDH 60

Query: 65  AQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL 124
           AQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL
Sbjct: 61  AQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL 119

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BLAST of Carg08823 vs. ExPASy Swiss-Prot
Match: P16048 (Glycine cleavage system H protein, mitochondrial OS=Pisum sativum OX=3888 GN=GDCSH PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.2e-50
Identity = 99/123 (80.49%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANAL+LSSSS+ HLSP FSISRCFS VLDGLKYA SHEWVKHEG VA IG
Sbjct: 1   MALRMWASSTANALKLSSSSRLHLSPTFSISRCFSNVLDGLKYAPSHEWVKHEGSVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFV+LPE G  V++GK FGAVESVKATSD+NSPISGE++EVN  L   
Sbjct: 61  ITDHAQDHLGEVVFVELPEPGVSVTKGKGFGAVESVKATSDVNSPISGEVIEVNTGLTGK 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

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BLAST of Carg08823 vs. ExPASy Swiss-Prot
Match: Q9LQL0 (Glycine cleavage system H protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GDH3 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 2.5e-48
Identity = 98/124 (79.03%), Postives = 112/124 (90.32%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSS-SKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAI 60
           MALRMW SSTANAL+LSSS SK+HL P FSISRCFS+VL+GLKYA+SHEWVKHEG VA I
Sbjct: 1   MALRMWASSTANALKLSSSASKSHLLPAFSISRCFSSVLEGLKYANSHEWVKHEGSVATI 60

Query: 61  GITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKD 120
           GITDHAQDHLGEVVFV+LPE+ + VS+ KSFGAVESVKATS+I SPISGE++EVN +L +
Sbjct: 61  GITDHAQDHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLTE 120

Query: 121 SPGL 124
           SPGL
Sbjct: 121 SPGL 124

[Close]

BLAST of Carg08823 vs. ExPASy Swiss-Prot
Match: Q39732 (Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala OX=35877 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 7.2e-48
Identity = 95/123 (77.24%), Postives = 108/123 (87.80%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSS+++ H SP   +SRCFS+VLDGLKYA+SHEWVKHEG VA IG
Sbjct: 1   MALRMWASSTANALRLSSATRPHFSP---LSRCFSSVLDGLKYANSHEWVKHEGSVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPE+G  V++   FGAVESVKATSD+NSPISGEIVEVN +L ++
Sbjct: 61  ITDHAQDHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSET 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 120

[Close]

BLAST of Carg08823 vs. ExPASy Swiss-Prot
Match: P49360 (Glycine cleavage system H protein, mitochondrial (Fragment) OS=Flaveria pubescens OX=35880 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.2e-47
Identity = 94/123 (76.42%), Postives = 108/123 (87.80%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSS+++ H SP   +SRCFS+VLDGLKYA+SHEWVKHEG VA +G
Sbjct: 1   MALRMWASSTANALRLSSATRPHYSP---LSRCFSSVLDGLKYANSHEWVKHEGSVATVG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPE+G  V++   FGAVESVKATSD+NSPISGEIVEVN +L ++
Sbjct: 61  ITDHAQDHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSET 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 120

[Close]

BLAST of Carg08823 vs. ExPASy Swiss-Prot
Match: P49359 (Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei OX=4226 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-47
Identity = 94/123 (76.42%), Postives = 108/123 (87.80%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALR+W SSTANALRLSS+++ H SP   +SRCFS+VLDGLKYA+SHEWVKHEG VA IG
Sbjct: 1   MALRIWASSTANALRLSSATRPHFSP---LSRCFSSVLDGLKYANSHEWVKHEGSVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPE+G  V++   FGAVESVKATSD+NSPISGEIVEVN +L ++
Sbjct: 61  ITDHAQDHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSET 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 120

[Close]

BLAST of Carg08823 vs. ExPASy TrEMBL
Match: A0A6J1ET51 (Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111437487 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.5e-59
Identity = 120/123 (97.56%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVA IG
Sbjct: 1   MALRMWASSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVN QLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNQQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. ExPASy TrEMBL
Match: A0A6J1JYK8 (Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111490918 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 5.5e-59
Identity = 120/123 (97.56%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSSSSKT LSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVA IG
Sbjct: 1   MALRMWASSTANALRLSSSSKTLLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVATIG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. ExPASy TrEMBL
Match: A0A6J1I8M1 (Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111470652 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.6e-56
Identity = 114/123 (92.68%), Postives = 117/123 (95.12%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSS+SKTHLS PFS+SRCFSTVLDGLKYASSHEWVKHEG VA +G
Sbjct: 1   MALRMWASSTANALRLSSASKTHLSSPFSLSRCFSTVLDGLKYASSHEWVKHEGEVATVG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESG  VSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. ExPASy TrEMBL
Match: A0A6J1GKG7 (Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111455190 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.6e-56
Identity = 114/123 (92.68%), Postives = 117/123 (95.12%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANALRLSS+SKTHLS PFS+SRCFSTVLDGLKYASSHEWVKHEG VA +G
Sbjct: 1   MALRMWASSTANALRLSSASKTHLSSPFSLSRCFSTVLDGLKYASSHEWVKHEGEVATVG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESG  VSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. ExPASy TrEMBL
Match: A0A0A0LI67 (Glycine cleavage system H protein OS=Cucumis sativus OX=3659 GN=Csa_3G903520 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.7e-56
Identity = 113/123 (91.87%), Postives = 118/123 (95.93%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAIG 60
           MALRMW SSTANAL+LSSSSKTHLSP FS+SRCFSTVLDGLKYASSHEWVKHEG VA +G
Sbjct: 1   MALRMWASSTANALKLSSSSKTHLSPSFSLSRCFSTVLDGLKYASSHEWVKHEGSVATVG 60

Query: 61  ITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDS 120
           ITDHAQDHLGEVVFVDLPESG+ VSQGKSFGAVESVKATSDINSPISGEIVEVNPQLK+S
Sbjct: 61  ITDHAQDHLGEVVFVDLPESGSSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKES 120

Query: 121 PGL 124
           PGL
Sbjct: 121 PGL 123

[Close]

BLAST of Carg08823 vs. TAIR 10
Match: AT1G32470.1 (Single hybrid motif superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 1.8e-49
Identity = 98/124 (79.03%), Postives = 112/124 (90.32%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSS-SKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAI 60
           MALRMW SSTANAL+LSSS SK+HL P FSISRCFS+VL+GLKYA+SHEWVKHEG VA I
Sbjct: 1   MALRMWASSTANALKLSSSASKSHLLPAFSISRCFSSVLEGLKYANSHEWVKHEGSVATI 60

Query: 61  GITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKD 120
           GITDHAQDHLGEVVFV+LPE+ + VS+ KSFGAVESVKATS+I SPISGE++EVN +L +
Sbjct: 61  GITDHAQDHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLTE 120

Query: 121 SPGL 124
           SPGL
Sbjct: 121 SPGL 124

[Close]

BLAST of Carg08823 vs. TAIR 10
Match: AT2G35370.1 (glycine decarboxylase complex H )

HSP 1 Score: 187.6 bits (475), Expect = 7.4e-48
Identity = 99/124 (79.84%), Postives = 109/124 (87.90%), Query Frame = 0


Query: 1   MALRMWVSSTANALRLSSS-SKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAI 60
           MALRMW SSTANAL+LSSS SK+HLS PFS SRCFSTVL+GLKYA+SHEWVKHEG VA I
Sbjct: 1   MALRMWASSTANALKLSSSVSKSHLS-PFSFSRCFSTVLEGLKYANSHEWVKHEGSVATI 60

Query: 61  GITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKD 120
           GIT HAQDHLGEVVFV+LPE    VS+ KSFGAVESVKATS+I SPISGEI+EVN +L +
Sbjct: 61  GITAHAQDHLGEVVFVELPEDNTSVSKEKSFGAVESVKATSEILSPISGEIIEVNKKLTE 120

Query: 121 SPGL 124
           SPGL
Sbjct: 121 SPGL 123

[Close]

BLAST of Carg08823 vs. TAIR 10
Match: AT2G35120.1 (Single hybrid motif superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 1.5e-32
Identity = 76/124 (61.29%), Postives = 91/124 (73.39%), Query Frame = 0


Query: 1   MALRM-WVSSTANALRLSSSSKTHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGPVAAI 60
           MA R+ W S  A+ LR+S + +      FS     S VL  LKYA SHEWVK +G  A  
Sbjct: 1   MACRLFWASRVASHLRISVAQR-----GFS-----SVVLKDLKYADSHEWVKIDGNKATF 60

Query: 61  GITDHAQDHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKD 120
           GITDHAQDHLG+VV+V+LP+ G  VSQGKSFGAVESVKATSDINSP+SG++VEVN +L +
Sbjct: 61  GITDHAQDHLGDVVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTE 114

Query: 121 SPGL 124
           SPGL
Sbjct: 121 SPGL 114

The following BLAST results are available for this feature:

BLAST of Carg08823 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036177.1	1.3e-78	100.00	Glycine cleavage system H protein, mitochondrial [Cucurbita argyrosperma subsp. ...	[more]
XP_023532627.1	6.1e-60	98.37	glycine cleavage system H protein 3, mitochondrial-like [Cucurbita pepo subsp. p...	[more]
XP_022931257.1	5.1e-59	97.56	glycine cleavage system H protein, mitochondrial-like [Cucurbita moschata]	[more]
XP_022995342.1	1.1e-58	97.56	glycine cleavage system H protein 3, mitochondrial-like [Cucurbita maxima]	[more]
KAG6606235.1	1.5e-58	99.16	Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperm...	[more]

BLAST of Carg08823 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16048	1.2e-50	80.49	Glycine cleavage system H protein, mitochondrial OS=Pisum sativum OX=3888 GN=GDC...	[more]
Q9LQL0	2.5e-48	79.03	Glycine cleavage system H protein 3, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
Q39732	7.2e-48	77.24	Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala OX=35877 GN...	[more]
P49360	1.2e-47	76.42	Glycine cleavage system H protein, mitochondrial (Fragment) OS=Flaveria pubescen...	[more]
P49359	2.1e-47	76.42	Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei OX=4226 GN...	[more]

BLAST of Carg08823 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ET51	2.5e-59	97.56	Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111437487 ...	[more]
A0A6J1JYK8	5.5e-59	97.56	Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111490918 PE...	[more]
A0A6J1I8M1	2.6e-56	92.68	Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111470652 PE...	[more]
A0A6J1GKG7	2.6e-56	92.68	Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111455190 ...	[more]
A0A0A0LI67	5.7e-56	91.87	Glycine cleavage system H protein OS=Cucumis sativus OX=3659 GN=Csa_3G903520 PE=...	[more]

BLAST of Carg08823 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32470.1	1.8e-49	79.03	Single hybrid motif superfamily protein	[more]
AT2G35370.1	7.4e-48	79.84	glycine decarboxylase complex H	[more]
AT2G35120.1	1.5e-32	61.29	Single hybrid motif superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033753	Glycine cleavage system H-protein/Simiate	PFAM	PF01597	GCV_H	coord: 41..123 e-value: 2.2E-31 score: 108.3
IPR033753	Glycine cleavage system H-protein/Simiate	CDD	cd06848	GCS_H	coord: 43..123 e-value: 5.25202E-42 score: 133.043
None	No IPR available	GENE3D	2.40.50.100		coord: 24..131 e-value: 1.2E-35 score: 123.9
None	No IPR available	PANTHER	PTHR11715:SF32	GLYCINE CLEAVAGE SYSTEM H PROTEIN	coord: 1..123
IPR002930	Glycine cleavage system H-protein	PANTHER	PTHR11715	GLYCINE CLEAVAGE SYSTEM H PROTEIN	coord: 1..123
IPR003016	2-oxo acid dehydrogenase, lipoyl-binding site	PROSITE	PS00189	LIPOYL	coord: 81..110
IPR000089	Biotin/lipoyl attachment	PROSITE	PS50968	BIOTINYL_LIPOYL	coord: 56..136 score: 22.634499
IPR011053	Single hybrid motif	SUPERFAMILY	51230	Single hybrid motif	coord: 37..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08823-RA	Carg08823-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019464	glycine decarboxylation via glycine cleavage system
biological_process	GO:0009249	protein lipoylation
cellular_component	GO:0005960	glycine cleavage complex
cellular_component	GO:0005739	mitochondrion