Carg08429 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08429
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein TPX2-like isoform X3
LocationCarg_Chr02: 5768863 .. 5774455 (+)
RNA-Seq ExpressionCarg08429
SyntenyCarg08429
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCTCTTTCTTCTTGTTCCTTCCTTCCTTCCCTCCCTCCCTCCATGGCTATGGCTTCCTCGTCCAAACTCTAGCGCCGGCTACTTCCACTTCATACACACATTCCTTCTAATGTTATGATTTTCTTTCTGTTCTTTCTTTTCCGCTTTTCGATCATGGCGAATTGATGTTTGTCTCGAGTTATGAATCGAATGTCCGCAGATTTTTCTTGCGGACAGCGGAGAGATTCGAGATGAGGGAATGTTGTAGAGATATTCGTTGTTTATTCTGCTTTTTTGGTTGCGTATTTCTGTGAAGATAAACAATTCCTGGTTCATTCGTCGTTTTCCATAGAAGAAAAACGGTAGAACGACGTTTGATGATATCCATGGACTTTTTCTGATTTCTTCGCGTTTACGTTGATATTATGGCGCTTTGTATGGAGAAGGAGAAGGAGACGGAGATGGAGATGGAGATGGAGATGGATGAAGAAATGGAAGAGGTTACTGACCAGCCATTTGATTCGGATGAGATTGATTTGGATTACGAGTTTGATGCCGCCATGTATTACGATTTTACTCGCCCCGAGACAGAGATGGAGATGAGAGGAGCCGAGGATTGGTTTAAATTCGCTGGAACCTATCCACCTTCTCGTAAGCATTTTGTTTTCTTATGGATCATTTGCCTCTTCGCGATTTCGATTTTATTGGATATCTGTTACAAATGTTGAAGTTATGGAATTATTCTGTTCTGTGGAATTTGATTTTTGACGACAACGCTTTCTCTTGGAAGAGTTTTTAGTGCATTATGGTTTCATGGGAATAAAATCCAATTATTTTGGAGAGTAAATCAAAATCTAATTGGTCTTTCATGTTGATTCTTATGCCATGATTTTTTCTTTCGTCTTTTAGCATTCGTCGTAAAATTGACTGGGGAAAAAGAGGCGCGGGCAGAGTGCACCTCTATTGAACTCAAAGGTGTCCAGAACAACCGGGAGGACCCTGAAAATTCAGGTATAATGTGCATTTCTTCTTTTATTATTCGTTCAGTTGGATTCAACCTCGGCAATATGCTGTGCCAGAACAGCTATTTTAATAGTTAACGATGAATATGATGGTGAAACAATATCAAATTATAAATCTTATGATGTGGCTGAAAAACGCCCCTGCAATCCAGACATCACTATCCTGAATTTCTGAATCTTTTTTCTTTCTTCAGTTCAAACAAGTTATATTAATGGTGCGATCTTCCTAAATTGGTCAGACTACTATCATGGCATATAAGGCCCAAGTATTTATTTTTGTGATGATTCAGCGTGGGTAGGATTTTGTATACTTCATCACCTAGGTGGCTATTCCAAACCAAAGTTTTTTCCGTAATTTTTTTGTGATATTTGCGTGTTGCAGTTTTTCTCCCTTTGAAATGGTTTTGATTAAAGGAGTTGCAGGCAACAGTTTTATTGTAATTCATTCGTTAGAATTATACTCTTGTTTGGAAGATCCTGTTCTGCTTATTTACCTTTTTATACTTAGGAATGTAATTACCCGAAATATTTTCATCCCTCTTAACTCCCATCCTACAATTAACTGGCTCAAGACATTATAGGAATTAATATGGAGTCTACTGTCAAGGTAAATCTACCAAGGTGTTCAAGTTTTATGAAACCAACTGCAAGCTATTTGGCCAAGCAAAATCAACCTCGAGGAATACACTCCACCACAGTTTTGCGAAGGTGATGATCATATCTTAAAATAATTCTTGACTGGCATTAATGATGTTTGTAGTCTTTTCAAAGCTTACTCTTGAAATATGATGGGGCCATTTCCCATTCCATCCCATGAAGGTAGGCTATATCAGGATTGTGAGTAATAGCTGTTGCTCATTCAATATTTTGGAACCTGTTCTGGTGAAAGAAGGGCTGTAAGCTCTTCAAATGATCTTCTGCATAACCACTATTTATTTTTAATCTTTATTTTATGTACTTTTCAATTTTTTAATCACTGGCCATTTTTTACTTCTTAGATTTCAGTTCAGCTTGGGCGATATTGTAGAGGCCAGTTCACAGCGTTCCTCGTTGAATATTAGTCATGCTACCAAGAGGCAAAAGTTGGAGGCTGGTTATTTTCCCAATGTGTGTGGAAACTTCTATTTCAACTTTTTCTATTTTGAATTGGCATTCTACCCATCTTCTAGAAATGTTTGTCTTTGTAGAGGGAAGAGAATGAAGCATTAGTTTTTGACCCGGATACGAGGAGACAGTATTAAACTTCATGTGTTGAAATCAAATATTTTTTTTAATAGTGTGTCGATCTTTTCTGCTTGCATGAGAGGTTATGAAACTCATTATTTGAGATGGTTCAGTTACCTCAGTCAGTAATTACAATTATTTGTTTCTCTTGCGTTCTTATATAGTACTGATAACCATTCAAACATTATTTGAAGATGCTGTGAAGGATATTAAGACTTAACTGATGGGATCCTAGAGTGCTGTCCAGTCGCCAGGACGTATTTTCTGATTGGCATATTGTTTCCATTTAATCATGGTTATTGCTTTCTTTGATTCGTCTCCCACTTTGTAACAGCATTTCTGAAGCATATTTGAGTACTTCATCTTGGAATTTGTTTCTTTCCGCTGTCAGCTTCTTTCATTCATTTTCCTAGATTATAAATAAATAAATAATACAAAAAAAATATTTTAAATTTTTTTTTAAGAAAAGCAAAAAAGAAAAGACAATTTATTTATATTTAAGCTGTTTTTGTAGATCTGGCAGTTAGTCAAGTTCCAGCCTAACCTGCAGAGTGAGTCCATTGATATCATAAGTTTTTAAGTAACTTTTTTGCTGCAGGTCCCACGTTTGATGCGTCAAGCAAGTTTGCAACACAAGGTGCCTAAAGAGGTAAGTGATGTGTACCTGGAAACTGGAATCGTTACTAACAGTTCATTTGAATTAATTTACCATGCATCTTCAGCCACTATTAACATACTTAGGCATATGCAAACTTGTTATAACGGTAGTATAATGTATGTTCTTAGTTCGTTCATCCACTAGAATTTAACCTACATGGCTACTACAGGTTGTTTCCGAAGCTAATATAGCTTTCGCCAGATTTAAATCTACCATACCTAAAGAGCCTAACCTTGAGACAGCAATTAGAGCACAAAGATGCAGGTACTCAGCTTTATTTGAATAAAAATAAAGTTTTTCTTTGTGATTGGCACAATTTCTTTTACCTCAGATGTTATTCATATAAGATGTATTCCATAGTTTTTGTGATCTTATTACCCTTTTTTGACTAAGCAGATCCTTGAGGCTCCTACTTTGCTCCCACCAAAGAAGAGCAAACCACAAATACCTGAGTTTCAAGTAAAATGTTTAGCATGTATTGTTTTCATTATTTGTGCTGATATATGTACCAGCGTCTTGATGAAAATTCTTGATTTACTTTAGGGTAATGGGCAAGTATTCTGCACCATAATTTTATTCACAGCTTTTATTTGATTGTTTTAGGTGTTTCGCCTTAAGACATCAGAGAGGGCTACAGAGCACACATATGATGTAAGCCATCAGACTGTTCTACAATCTTAGCTGAGTCTATGTCATCGATTTTGTGAACAATTTGAAATTTTTTGTTTCTCCTTTAATTGTTACTAAAAATTTCTTGTAGCACTGATGCTCCAACAATTGCAGGTAAATAATCCTTGTGTATCCAATCCACAAATACGAAACAAATGCACAAGAAGGTAATTGAGGTAGACCTTTCAGCAATTGTAAATTTATCGTGTAAAAGACCTTTACTCTGAAGATTTAATTAATGACTATAATTTTAGTAACTACGAAGCATATTTCCGTTGCTTTGAGCCTGTTTAACTTATTGGTGATCGTATCAGTGACCGTATGCATATTCTAGATTTATTAGTAAGATTAATTAACTGAATCTACGATTATTTTCATTTACTTTTTGTTAATTCACCGGGAGATGTTACTGATGTTTATCATTTATTCTTAATATTGTTGATCTTTTACATCTCACGTGCAACAGACCGAGTTCTCTCCAAGATACGAAACAAGAGAAATGTGTAAAAGCTGAAAGATGTAAAGCTCGGCCACTTGATAAGGTTGGAATTCGTAACAACGCTGCAATTCTTTGTTTTCTCAACATTTGAATGTAATGAGCTCTAAGCTCTTTCATACAGAGACTCTCACATAATGATGAAGAATGTGATGTGGTTGCGAATTGTAAAGATGGAACAACTGTGTCCACGGTAAGCCTCATTTTGCCCCCATATATATTGAATTTCAAGTTTATTCCATTCAGTTCTAAGTTTCGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGGAGTATTTTAGGAATTTAGCTGTGTAGATCAAAAGAAAGTTTCGGACGAGCCACCAACTAAATTACTTAGCAAGGTAGTACCCAAATACTGTGCATCTCAAGTTTTGTTCATCATTTAAGATTTAACCTTCTGTTATAAAATAAAGATTCTAAAGACTAACCTGTTCTATTTGTTTCAACAATTTCGTATCATTGACAGCTCTCCCTTTCATCAGAATGTCGTCGATGCAATACAAAATCTTCTTGCAAAATGCATTTTTCGGACAATGTTAGCTTTTTTCATCGTTTTCACTCCCATTATTAACTGAATCATCTTCTTCTTACACTCAAAAAGTGTTTTATTTCTTATTTGTTAGTGCTCAAAAGAGAATGTCCCAGGCTTCCTTGATCAAGAGCGTGAGGTATATTACAAAAGTTTTAATTAGAGTTTAAAGGTCTTTTTTTTTCTATTCTCATCGACTCATTGCATTCAAGTTGTAGGTTGTTGATATACTTTTGCCAAATTCCCGAGGTCTTTTAGCAAAGTGAAAACCAAAACCTTGAGATGATTCTACCAGGTAACCTTCCCTAACTATTTTGTTTTTGTTTTATATTCTTAAATGAATTAAGCCTTACTCATACCTCTCTTAACTGAATAATAAAATGTAAATAATTAGGTAATGCTGTCTTAAGTTTGGAAAGCTAAAAATAAAATCCTGTAATTTTAAGTCAATCCGATTTAATTTTGTTTTTCCCCTTTCTTTTTTATCGGTGTTAATATTAACATTTTTATTTATTTATATTTATGATATTTTTTCTTTTGGCCTCAAATATCAAAATGAATAATGATTTATCATTATATATATATATATATATATATATATCATTAATCTACTCAATGCCAGCAATTATAAATTTATATTCTTACAAAAAAAAACAATGCCAGCAATTATAAATTTATATTCTTACAAAAAAAAAAAATCTCACCTTCAAAAGTCTTTTCTTTAGAGAATTTCAACTGTTCTATGTAACCAAGAATTATTGTAGAAGCGAATTAAAGGTTTTGCTATTTGTTGAAAAATGAATTTTACAACAAATAGTTCTAAAACATATATATTCTGAGGTATAGAATTTTTTTGCTTACAAAATATTCCATTTCATTGCAGTTTCTTCATTCTTCATTCTTCATTCCACCCAATGAGCTCATTGATGTTCTAGTTCTGGCAACATATGACATGAGGCAATTTTTTGGGTAG

mRNA sequence

CTTCCTCTTTCTTCTTGTTCCTTCCTTCCTTCCCTCCCTCCCTCCATGGCTATGGCTTCCTCGTCCAAACTCTAGCGCCGGCTACTTCCACTTCATACACACATTCCTTCTAATGTTATGATTTTCTTTCTGTTCTTTCTTTTCCGCTTTTCGATCATGGCGAATTGATGTTTGTCTCGAGTTATGAATCGAATGTCCGCAGATTTTTCTTGCGGACAGCGGAGAGATTCGAGATGAGGGAATGTTGTAGAGATATTCGTTGTTTATTCTGCTTTTTTGGTTGCGTATTTCTGTGAAGATAAACAATTCCTGGTTCATTCGTCGTTTTCCATAGAAGAAAAACGGTAGAACGACGTTTGATGATATCCATGGACTTTTTCTGATTTCTTCGCGTTTACGTTGATATTATGGCGCTTTGTATGGAGAAGGAGAAGGAGACGGAGATGGAGATGGAGATGGAGATGGATGAAGAAATGGAAGAGGTTACTGACCAGCCATTTGATTCGGATGAGATTGATTTGGATTACGAGTTTGATGCCGCCATGTATTACGATTTTACTCGCCCCGAGACAGAGATGGAGATGAGAGGAGCCGAGGATTGGTTTAAATTCGCTGGAACCTATCCACCTTCTCCATTCGTCGTAAAATTGACTGGGGAAAAAGAGGCGCGGGCAGAGTGCACCTCTATTGAACTCAAAGGTGTCCAGAACAACCGGGAGGACCCTGAAAATTCAGGTATAATGTGCATTTCTTCTTTTATTATTCGTTCAGTTGGATTCAACCTCGGCAATATGCTGTGCCAGAACAGCTATTTTAATAACATTATAGGAATTAATATGGAGTCTACTGTCAAGGTAAATCTACCAAGGTGTTCAAGTTTTATGAAACCAACTGCAAGCTATTTGGCCAAGCAAAATCAACCTCGAGGAATACACTCCACCACAGTTTTGCGAAGATTTCAGTTCAGCTTGGGCGATATTGTAGAGGCCAGTTCACAGCGTTCCTCGTTGAATATTAGTCATGCTACCAAGAGGCAAAAGTTGGAGGCTGGTTATTTTCCCAATGTGTGTGGAAACTTCTATTTCAACTTTTTCTATTTTGAATTGGCATTCTACCCATCTTCTAGAAATGTCCCACGTTTGATGCGTCAAGCAAGTTTGCAACACAAGGTGCCTAAAGAGATCCTTGAGGCTCCTACTTTGCTCCCACCAAAGAAGAGCAAACCACAAATACCTGAGTTTCAAGTGTTTCGCCTTAAGACATCAGAGAGGGCTACAGAGCACACATATGATGTAAATAATCCTTGTGTATCCAATCCACAAATACGAAACAAATGCACAAGAAGACCGAGTTCTCTCCAAGATACGAAACAAGAGAAATGTGTAAAAGCTGAAAGATGTAAAGCTCGGCCACTTGATAAGAGACTCTCACATAATGATGAAGAATGTGATGTGGTTGCGAATTGTAAAGATGGAACAACTGTGTCCACGGAATTTAGCTGTGTAGATCAAAAGAAAGTTTCGGACGAGCCACCAACTAAATTACTTAGCAAGCTCTCCCTTTCATCAGAATGTCGTCGATGCAATACAAAATCTTCTTGCAAAATGCATTTTTCGGACAATTGCTCAAAAGAGAATGTCCCAGGCTTCCTTGATCAAGAGCGTGAGTTTCTTCATTCTTCATTCTTCATTCCACCCAATGAGCTCATTGATGTTCTAGTTCTGGCAACATATGACATGAGGCAATTTTTTGGGTAG

Coding sequence (CDS)

ATGGCGCTTTGTATGGAGAAGGAGAAGGAGACGGAGATGGAGATGGAGATGGAGATGGATGAAGAAATGGAAGAGGTTACTGACCAGCCATTTGATTCGGATGAGATTGATTTGGATTACGAGTTTGATGCCGCCATGTATTACGATTTTACTCGCCCCGAGACAGAGATGGAGATGAGAGGAGCCGAGGATTGGTTTAAATTCGCTGGAACCTATCCACCTTCTCCATTCGTCGTAAAATTGACTGGGGAAAAAGAGGCGCGGGCAGAGTGCACCTCTATTGAACTCAAAGGTGTCCAGAACAACCGGGAGGACCCTGAAAATTCAGGTATAATGTGCATTTCTTCTTTTATTATTCGTTCAGTTGGATTCAACCTCGGCAATATGCTGTGCCAGAACAGCTATTTTAATAACATTATAGGAATTAATATGGAGTCTACTGTCAAGGTAAATCTACCAAGGTGTTCAAGTTTTATGAAACCAACTGCAAGCTATTTGGCCAAGCAAAATCAACCTCGAGGAATACACTCCACCACAGTTTTGCGAAGATTTCAGTTCAGCTTGGGCGATATTGTAGAGGCCAGTTCACAGCGTTCCTCGTTGAATATTAGTCATGCTACCAAGAGGCAAAAGTTGGAGGCTGGTTATTTTCCCAATGTGTGTGGAAACTTCTATTTCAACTTTTTCTATTTTGAATTGGCATTCTACCCATCTTCTAGAAATGTCCCACGTTTGATGCGTCAAGCAAGTTTGCAACACAAGGTGCCTAAAGAGATCCTTGAGGCTCCTACTTTGCTCCCACCAAAGAAGAGCAAACCACAAATACCTGAGTTTCAAGTGTTTCGCCTTAAGACATCAGAGAGGGCTACAGAGCACACATATGATGTAAATAATCCTTGTGTATCCAATCCACAAATACGAAACAAATGCACAAGAAGACCGAGTTCTCTCCAAGATACGAAACAAGAGAAATGTGTAAAAGCTGAAAGATGTAAAGCTCGGCCACTTGATAAGAGACTCTCACATAATGATGAAGAATGTGATGTGGTTGCGAATTGTAAAGATGGAACAACTGTGTCCACGGAATTTAGCTGTGTAGATCAAAAGAAAGTTTCGGACGAGCCACCAACTAAATTACTTAGCAAGCTCTCCCTTTCATCAGAATGTCGTCGATGCAATACAAAATCTTCTTGCAAAATGCATTTTTCGGACAATTGCTCAAAAGAGAATGTCCCAGGCTTCCTTGATCAAGAGCGTGAGTTTCTTCATTCTTCATTCTTCATTCCACCCAATGAGCTCATTGATGTTCTAGTTCTGGCAACATATGACATGAGGCAATTTTTTGGGTAG

Protein sequence

MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMRGAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIRSVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTVLRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSRNVPRLMRQASLQHKVPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVSNPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTEFSCVDQKKVSDEPPTKLLSKLSLSSECRRCNTKSSCKMHFSDNCSKENVPGFLDQEREFLHSSFFIPPNELIDVLVLATYDMRQFFG
Homology
BLAST of Carg08429 vs. NCBI nr
Match: KAG7035616.1 (Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.9 bits (2319), Expect = 3.6e-257
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
           SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240

Query: 241 NVPRLMRQASLQHKVPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPC 300
           NVPRLMRQASLQHKVPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPC
Sbjct: 241 NVPRLMRQASLQHKVPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPC 300

Query: 301 VSNPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVS 360
           VSNPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVS
Sbjct: 301 VSNPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVS 360

Query: 361 TEFSCVDQKKVSDEPPTKLLSKLSLSSECRRCNTKSSCKMHFSDNCSKENVPGFLDQERE 420
           TEFSCVDQKKVSDEPPTKLLSKLSLSSECRRCNTKSSCKMHFSDNCSKENVPGFLDQERE
Sbjct: 361 TEFSCVDQKKVSDEPPTKLLSKLSLSSECRRCNTKSSCKMHFSDNCSKENVPGFLDQERE 420

Query: 421 FLHSSFFIPPNELIDVLVLATYDMRQFFG 450
           FLHSSFFIPPNELIDVLVLATYDMRQFFG
Sbjct: 421 FLHSSFFIPPNELIDVLVLATYDMRQFFG 449

BLAST of Carg08429 vs. NCBI nr
Match: KAG6605707.1 (Protein TPX2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.1 bits (1738), Expect = 8.4e-190
Identity = 367/478 (76.78%), Postives = 367/478 (76.78%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420
           NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE
Sbjct: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420

BLAST of Carg08429 vs. NCBI nr
Match: XP_022958386.1 (uncharacterized protein LOC111459624 isoform X2 [Cucurbita moschata])

HSP 1 Score: 672.2 bits (1733), Expect = 3.2e-189
Identity = 366/478 (76.57%), Postives = 366/478 (76.57%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420
           NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE
Sbjct: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420

BLAST of Carg08429 vs. NCBI nr
Match: XP_022958385.1 (uncharacterized protein LOC111459624 isoform X1 [Cucurbita moschata])

HSP 1 Score: 667.5 bits (1721), Expect = 7.9e-188
Identity = 366/479 (76.41%), Postives = 366/479 (76.41%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTR-RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420
           NPQIRNKCTR RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST
Sbjct: 361 NPQIRNKCTRSRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420

BLAST of Carg08429 vs. NCBI nr
Match: XP_022958388.1 (uncharacterized protein LOC111459624 isoform X3 [Cucurbita moschata])

HSP 1 Score: 667.5 bits (1721), Expect = 7.9e-188
Identity = 366/479 (76.41%), Postives = 366/479 (76.41%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTR-RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420
           NPQIRNKCTR RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST
Sbjct: 361 NPQIRNKCTRSRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420

BLAST of Carg08429 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-08
Identity = 50/154 (32.47%), Postives = 78/154 (50.65%), Query Frame = 0

Query: 255 VPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEH--TYDVNNPCVS---------- 314
           V K+IL AP L  P++S P +PEFQ F L+T  RA +H  T  + +  VS          
Sbjct: 358 VNKKILAAPALPAPQRSTPHLPEFQEFHLQTMARANQHAETSSIASTEVSKQHNDQKHHL 417

Query: 315 ----NPQIRNKCTRRPSSLQDT---KQEKCVKAERCKARPLDKRLSHNDEECDVVANCKD 374
               +P ++     RP+  + T   +QE+  KA + KA+PL+K++  +  E  +  N K 
Sbjct: 418 TEPKSPVLQTMLRARPTIAKTTAELEQEELEKAPKFKAKPLNKKIFESKGEMGIFCNTKK 477

Query: 375 GTTVSTEFSCVDQKKVS-DEPPTKLLSKLSLSSE 389
             T+  EF     +++S  E    +  KLSL+SE
Sbjct: 478 HITIPQEFHFATDERISRPESVLDIFDKLSLNSE 511


HSP 2 Score: 43.5 bits (101), Expect = 7.3e-03
Identity = 22/55 (40.00%), Postives = 31/55 (56.36%), Query Frame = 0

Query: 36 IDLDYEFDAAMYYDFTRPETEMEMRGAEDWFKFAGTYPPSPFVVKLTGEKEARAE 91
          +D  YEF A  ++DF   ETE E R AE WF+ A +  PSP V ++   +  + E
Sbjct: 17 VDETYEFLAPRWFDFVNGETEDESRRAELWFQSALSCAPSPSVPRIKARRSFKVE 71

BLAST of Carg08429 vs. ExPASy TrEMBL
Match: A0A6J1H1Y0 (uncharacterized protein LOC111459624 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459624 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.5e-189
Identity = 366/478 (76.57%), Postives = 366/478 (76.57%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420
           NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE
Sbjct: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420

BLAST of Carg08429 vs. ExPASy TrEMBL
Match: A0A6J1H3B2 (uncharacterized protein LOC111459624 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459624 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.8e-188
Identity = 366/479 (76.41%), Postives = 366/479 (76.41%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTR-RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420
           NPQIRNKCTR RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST
Sbjct: 361 NPQIRNKCTRSRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420

BLAST of Carg08429 vs. ExPASy TrEMBL
Match: A0A6J1H2Y7 (uncharacterized protein LOC111459624 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459624 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.8e-188
Identity = 366/479 (76.41%), Postives = 366/479 (76.41%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR
Sbjct: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360

Query: 361 NPQIRNKCTR-RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420
           NPQIRNKCTR RPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST
Sbjct: 361 NPQIRNKCTRSRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVST 420

BLAST of Carg08429 vs. ExPASy TrEMBL
Match: A0A6J1K915 (uncharacterized protein LOC111491222 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491222 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.6e-181
Identity = 356/478 (74.48%), Postives = 357/478 (74.69%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKE E EMEM MEMDEEMEEVTDQPF SDEIDLDYEFDAAMYYDFTRPETE EMR
Sbjct: 1   MALCMEKETEMEMEMGMEMDEEMEEVTDQPFYSDEIDLDYEFDAAMYYDFTRPETETEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVE SSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEPSSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVN+PCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNSPCVS 360

Query: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420
           NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHN EECDVVANCKDGTTVSTE
Sbjct: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHN-EECDVVANCKDGTTVSTE 420

BLAST of Carg08429 vs. ExPASy TrEMBL
Match: A0A6J1K2W6 (uncharacterized protein LOC111491222 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491222 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.6e-181
Identity = 356/478 (74.48%), Postives = 357/478 (74.69%), Query Frame = 0

Query: 1   MALCMEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMR 60
           MALCMEKE E EMEM MEMDEEMEEVTDQPF SDEIDLDYEFDAAMYYDFTRPETE EMR
Sbjct: 1   MALCMEKETEMEMEMGMEMDEEMEEVTDQPFYSDEIDLDYEFDAAMYYDFTRPETETEMR 60

Query: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIR 120
           GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS           
Sbjct: 61  GAEDWFKFAGTYPPSPFVVKLTGEKEARAECTSIELKGVQNNREDPENS----------- 120

Query: 121 SVGFNLGNMLCQNSYFNNIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180
                               GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV
Sbjct: 121 --------------------GINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTV 180

Query: 181 LRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSR 240
           LRRFQFSLGDIVE SSQRSSLNISHATKRQKLEAGYFP                      
Sbjct: 181 LRRFQFSLGDIVEPSSQRSSLNISHATKRQKLEAGYFP---------------------- 240

Query: 241 NVPRLMRQASLQHKVPKE------------------------------------------ 300
           NVPRLMRQASLQHKVPKE                                          
Sbjct: 241 NVPRLMRQASLQHKVPKEVVSEANIAFARFKSTIPKEPNLETAIRAQRCRSKINSTASEH 300

Query: 301 ----------------ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCVS 360
                           ILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVN+PCVS
Sbjct: 301 NKSNASNFKARPLNRKILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNSPCVS 360

Query: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANCKDGTTVSTE 420
           NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHN EECDVVANCKDGTTVSTE
Sbjct: 361 NPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHN-EECDVVANCKDGTTVSTE 420

BLAST of Carg08429 vs. TAIR 10
Match: AT4G22860.1 (Cell cycle regulated microtubule associated protein )

HSP 1 Score: 133.3 bits (334), Expect = 5.0e-31
Identity = 146/480 (30.42%), Postives = 208/480 (43.33%), Query Frame = 0

Query: 17  MEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMRGAEDWFKFAGTYPPSP 76
           MEMDE+M E+    F+  EIDL+YEFDAA +YDFTR E   E + AE WF  A +Y PSP
Sbjct: 1   MEMDEDM-EIEPMVFEVKEIDLEYEFDAARWYDFTREELPEESQAAEFWFHSAPSYAPSP 60

Query: 77  FVVKLTGEKEARAECTSIELKGVQNNREDPENSGIMC----------------------- 136
           FV KL   +E   + T    +      ED E    +C                       
Sbjct: 61  FVTKLLLREEVYDDKTEASTRS-----EDEEVVADVCDKYKGTFQQPNHLATDFNKTGSR 120

Query: 137 ISSFIIRS---VGFNLGNMLCQNSYFNNIIGINMESTVKVNL---------PRCSSFMKP 196
           + S I  S    G NL  +  Q  +    +  N + T K            PR S+ M+P
Sbjct: 121 MRSGIFSSQQQQGCNLKKVPNQPIWKGPTVS-NHKHTDKPKFRAKSSIRPTPRSSTLMRP 180

Query: 197 TASYLAKQNQPRGIHSTTVLRRFQFSLGDIVEASSQRSSLNIS----HATKRQKLEAGYF 256
           TAS LAKQN     H               V+ S  + S   S     A+KRQKL+ G  
Sbjct: 181 TASQLAKQNNASKFHMQ-------------VDQSHDKGSCGTSGTEVQASKRQKLDGGLL 240

Query: 257 PNVCGNFYFNFFYFEL-----------------------AFYPSSRNVPRLMRQ--ASLQ 316
             V        F  ++                         + +S+   R+  +  A L+
Sbjct: 241 RKVADTKQEMSFVHKIPKKDTTLDKNAQHARTKITIPQEPDFATSQRAHRIRHKNDAKLE 300

Query: 317 HKVP-----------KEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHT-------- 376
            + P           ++I +AP+L   KKS P++PEFQ F LKTSERA +H+        
Sbjct: 301 QEQPAVYRFKARPFNRKIFDAPSLPIRKKSTPKLPEFQEFHLKTSERAMQHSSAVTTRSH 360

Query: 377 -YDVNNPCVSNPQI--RNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVV 411
            Y  ++  +    +   N+ +RRPS +  +K +    +   KARPL+K++  +  +  + 
Sbjct: 361 AYKESDKSIITDMLDGDNRESRRPSGMDISKHDVSEGSHVFKARPLNKKILSSRGDMGIF 420

BLAST of Carg08429 vs. TAIR 10
Match: AT4G11990.1 (Cell cycle regulated microtubule associated protein )

HSP 1 Score: 125.9 bits (315), Expect = 7.9e-29
Identity = 141/460 (30.65%), Postives = 210/460 (45.65%), Query Frame = 0

Query: 5   MEKEKETEMEMEMEMDEEMEEVTDQPFDSDEIDLDYEFDAAMYYDFTRPETEMEMRGAED 64
           ME + ETE + +MEM+  + EVT       EIDL+YEFDA+ ++DFTR E  +E R AE 
Sbjct: 3   METDTETESDEDMEMETMVFEVT-------EIDLEYEFDASRWFDFTREELPLESRAAEL 62

Query: 65  WFKFAGTYPPSPFVVKL---------TGEKEARAECTSIELKGVQNNREDPENSGI---- 124
           WF+ A +YPPSPF +KL           E  +++E   + ++  +++RE P+   +    
Sbjct: 63  WFETAQSYPPSPFAMKLLMIEEVYDEKTESLSKSEDGEVTVEVQESDREIPQQPDVNETG 122

Query: 125 MCISSFIIRSV-GFNLGNM----LCQNSYFNNIIGINM-----ESTVKVNLPRCSSFMKP 184
             + S + R + G  L  +    L +   F+N I  +      +S+++  + R S+ MKP
Sbjct: 123 NGMKSGVFRFIQGGTLSKVPNDSLHKGPTFSNRIHSDKLKYRPKSSIR-PITRSSTLMKP 182

Query: 185 TASYLAKQNQPRGIHSTTVLRRFQFSLGDIVEASSQRSSLNISHATKRQKLEAGYFPNVC 244
           TAS LAKQ+            R    + +I   SS+  S       KRQKL+ G    V 
Sbjct: 183 TASVLAKQDN----------ARLHMQVDEIKCLSSEIQS------AKRQKLDGGLLRKVA 242

Query: 245 -----GNFYFNFFYFEL----------AFYPSSRNVPRLMRQASLQHK------------ 304
                 N        +L             P   +     R   ++HK            
Sbjct: 243 EAAQETNLVHKALKKDLDKNSLHTRMKITIPQEPDFATSHRANRIRHKNDAKLEQDSTSV 302

Query: 305 -------VPKEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEH-------TYDVNN-- 364
                  + ++I EAP+L   K S P++PEFQ FRLKTSERA +H       +Y  NN  
Sbjct: 303 YRFKARPLNRKIFEAPSLPIRKNSTPKLPEFQEFRLKTSERAMQHSSAVSTSSYQGNNNH 362

Query: 365 -----PCVSNPQIRNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLSHNDEECDVVANC 394
                P  + P   N+  RR  ++   K +  V+ +  KARPL+K++  +  E  +    
Sbjct: 363 KEADKPNRTFPDGVNREPRRQRAIDIPKDD--VRKDLFKARPLNKKILSSGREIGMFTKS 422

BLAST of Carg08429 vs. TAIR 10
Match: AT5G07170.1 (Cell cycle regulated microtubule associated protein )

HSP 1 Score: 102.8 bits (255), Expect = 7.2e-22
Identity = 115/404 (28.47%), Postives = 170/404 (42.08%), Query Frame = 0

Query: 19  MDEEMEEVTDQPFDSD-EIDLDYEFDAAMYYDFTRPETEMEMRGAEDWFKFAGTYPPSPF 78
           MD   E+ + + FD D E D+D+EFDA  +YDF++PE + E    E WF+ AG YPPSPF
Sbjct: 17  MDIFAEDFSLEKFDIDFEFDIDFEFDAPRFYDFSKPELDSETEETELWFQSAGNYPPSPF 76

Query: 79  VVKLTGEKEARAECTSIELKGVQNNREDPENSGIMCISSFIIRSVGFNLGNMLCQNSYFN 138
                          SI L+  + + E P             + V       +  N    
Sbjct: 77  ---------------SIHLRYEEKHLEIP-------------KPVSDQYNGFIYNNQAPK 136

Query: 139 NIIGINMESTVKVNLPRCSSFMKPTASYLAKQNQPRGIHSTTVLRRFQFSLGDIVEA-SS 198
           ++     +S  K  L + S+  +PTAS LA+QN+P  I+S  +L R Q SLG   +  SS
Sbjct: 137 DVPKATHKSKTKTYLRKNSTLTRPTASLLARQNKPLDIYSVRLLTRCQRSLGKCDDKFSS 196

Query: 199 QRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFY--------FELAFYPSSRNVPRLMRQ 258
              S+  +   KRQKLE G+   V       F +          +   P+ +   R  R 
Sbjct: 197 HLYSMPQTQDNKRQKLETGFLRKVSRLDQTPFLHKVPKKGSKVTVPREPNLKTAQRAARN 256

Query: 259 ASLQHKVPKEILE-------------APTLLPPKKSKPQIP-EFQVFRLKTSERATEHTY 318
               +  P++I +             +P+L  PKK+ P  P + Q F L+TS RA E   
Sbjct: 257 RFKANSAPEQIAKFISNMNKTVQGTTSPSL--PKKNTPHHPQDLQAFHLRTSLRARER-- 316

Query: 319 DVNNPCVSNPQIRNKCTRRPSSLQDTKQEKCVKAERC----------KARPLDKRLSHND 378
             ++     P   +K +    S+   K  K VKA R           K  PLD ++    
Sbjct: 317 --SSSAKIAPTDDSKHSLTSKSVGSMKGMK-VKASRSSNSNCQVYGPKICPLDSKVMQTS 376

Query: 379 EECDVVANCKDGTTVSTEFSCVDQKKVSDEPPTKLLSKLSLSSE 389
            +C    + K    +  EF         DEP  + L KL L+S+
Sbjct: 377 SKCGEAIDIKHENNLLREFEAPMNTNFRDEPFIESLRKLCLTSD 385

BLAST of Carg08429 vs. TAIR 10
Match: AT5G62240.1 (Cell cycle regulated microtubule associated protein )

HSP 1 Score: 93.6 bits (231), Expect = 4.4e-19
Identity = 95/347 (27.38%), Postives = 149/347 (42.94%), Query Frame = 0

Query: 32  DSDEIDLDYEFDAAMYYDFTRPETEMEMRGAEDWFKFAGTYPPSPFVVKLTGEKEARAEC 91
           D  E D+D+EFDA  +YDF+RPE   E    E WF+ +G YPPSPF  K   + E   + 
Sbjct: 15  DDFEFDIDFEFDAPRFYDFSRPELYSETEEIEIWFQSSGNYPPSPFSPKFNWKFEPFKQI 74

Query: 92  TSI--ELKGVQNNREDPENSGIMCISSFIIRSVGFNLGNMLCQNSYFNNII-GINMESTV 151
           T+   E K V+        +G+ C   F     GF          Y+N  +  ++ + + 
Sbjct: 75  TNTISESKPVEIIESSEAETGLNCKDKF----NGF---------IYYNQTVKDVSKKKSK 134

Query: 152 KVNLPRCSSFMKPTASYLAKQNQPRGIHSTTVLRRFQFSLGDIVE--------------A 211
                  S+  +PTAS LA+QN+P  I+S  +L R Q SL    E               
Sbjct: 135 SKTKTSNSTLTRPTASLLARQNKPLDIYSVQLLTRCQRSLAKFGENVAPVLVSKLQNQDT 194

Query: 212 SSQRSSLNISHATKRQKLEAGYFPNVCGNFYFNFFYFELAFYPSSRNVPR--LMRQASLQ 271
           + Q+    ++H + R KL     PN+               + S  N  R  +    S +
Sbjct: 195 NRQKQEAKVAHVSSRAKLTVPKEPNL-------RTAERSERHRSKVNTQREQIATSNSKR 254

Query: 272 HKVPKEI-LEAPTLLPPKKSKPQIPEFQVFRLKTSERATEHTYDVNNPCV-SNPQIRNKC 331
           H   K I LE  +    K + P+   FQ FRL+TS RA E + +     V  N   +++ 
Sbjct: 255 HIRNKNINLEPVSTSLAKTNTPRSQHFQAFRLRTSLRAKERSSNAKTDVVQENDATKSRT 314

Query: 332 TRRPSSLQDTK--QEKCVKAERC-----KARPLDKRLSHNDEECDVV 351
            ++P     T+  +E   +   C     K+ PLD ++S  ++  + +
Sbjct: 315 LKKPIDSLKTRIVKENHSRKINCQVYESKSSPLDPKISSKEDLVEAI 341

BLAST of Carg08429 vs. TAIR 10
Match: AT4G22860.2 (Cell cycle regulated microtubule associated protein )

HSP 1 Score: 90.5 bits (223), Expect = 3.7e-18
Identity = 92/309 (29.77%), Postives = 138/309 (44.66%), Query Frame = 0

Query: 153 PRCSSFMKPTASYLAKQNQPRGIHSTTVLRRFQFSLGDIVEASSQRSSLNIS----HATK 212
           PR S+ M+PTAS LAKQN     H               V+ S  + S   S     A+K
Sbjct: 157 PRSSTLMRPTASQLAKQNNASKFHMQ-------------VDQSHDKGSCGTSGTEVQASK 216

Query: 213 RQKLEAGYFPNVCGNFYFNFFYFEL-----------------------AFYPSSRNVPRL 272
           RQKL+ G    V        F  ++                         + +S+   R+
Sbjct: 217 RQKLDGGLLRKVADTKQEMSFVHKIPKKDTTLDKNAQHARTKITIPQEPDFATSQRAHRI 276

Query: 273 MRQ--ASLQHKVP-----------KEILEAPTLLPPKKSKPQIPEFQVFRLKTSERATEH 332
             +  A L+ + P           ++I +AP+L   KKS P++PEFQ F LKTSERA +H
Sbjct: 277 RHKNDAKLEQEQPAVYRFKARPFNRKIFDAPSLPIRKKSTPKLPEFQEFHLKTSERAMQH 336

Query: 333 T---------YDVNNPCVSNPQI--RNKCTRRPSSLQDTKQEKCVKAERCKARPLDKRLS 392
           +         Y  ++  +    +   N+ +RRPS +  +K +    +   KARPL+K++ 
Sbjct: 337 SSAVTTRSHAYKESDKSIITDMLDGDNRESRRPSGMDISKHDVSEGSHVFKARPLNKKIL 396

Query: 393 HNDEECDVVANCKDGTTVSTEFSCVDQKKVSDEPPTKLLSKLSLSSECRRCNTKSSCKMH 411
            +  +  +  N K  TTV  EFS   +K+V  + PT L SKLS+ SE +  N  S  ++ 
Sbjct: 397 SSRGDMGIFKNSKRETTVPLEFSFHSEKRVQPDLPTDLFSKLSIKSEIQP-NDGSRTRL- 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035616.13.6e-257100.00Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6605707.18.4e-19076.78Protein TPX2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958386.13.2e-18976.57uncharacterized protein LOC111459624 isoform X2 [Cucurbita moschata][more]
XP_022958385.17.9e-18876.41uncharacterized protein LOC111459624 isoform X1 [Cucurbita moschata][more]
XP_022958388.17.9e-18876.41uncharacterized protein LOC111459624 isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4I2H71.2e-0832.47Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H1Y01.5e-18976.57uncharacterized protein LOC111459624 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H3B23.8e-18876.41uncharacterized protein LOC111459624 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H2Y73.8e-18876.41uncharacterized protein LOC111459624 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K9152.6e-18174.48uncharacterized protein LOC111491222 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1K2W62.6e-18174.48uncharacterized protein LOC111491222 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT4G22860.15.0e-3130.42Cell cycle regulated microtubule associated protein [more]
AT4G11990.17.9e-2930.65Cell cycle regulated microtubule associated protein [more]
AT5G07170.17.2e-2228.47Cell cycle regulated microtubule associated protein [more]
AT5G62240.14.4e-1927.38Cell cycle regulated microtubule associated protein [more]
AT4G22860.23.7e-1829.77Cell cycle regulated microtubule associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 5..25
NoneNo IPR availablePANTHERPTHR14326:SF15OS06G0130200 PROTEINcoord: 24..220
NoneNo IPR availablePANTHERPTHR14326:SF15OS06G0130200 PROTEINcoord: 255..399
IPR027330TPX2 central domainPFAMPF12214TPX2_importincoord: 253..364
e-value: 4.1E-17
score: 62.9
IPR009675TPX2PANTHERPTHR14326TARGETING PROTEIN FOR XKLP2coord: 255..399
IPR009675TPX2PANTHERPTHR14326TARGETING PROTEIN FOR XKLP2coord: 24..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08429-RACarg08429-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0060236 regulation of mitotic spindle organization
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle