Carg08409 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08409
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCytochrome p450
LocationCarg_Chr02: 5886435 .. 5888936 (+)
RNA-Seq ExpressionCarg08409
SyntenyCarg08409
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCTCTACTTCCTTGCTCTTCATGCTATTGTTTTCTTGTTGGATTTCACCTTTTTTAGCCCACAAAAAGCTAAAGCGAAATGGGTTTCGAGGTCCGACCCCGAGTTTTCCTCTCGGGAATATTAGCGAGATGAAAAGGACGAGCTTTGGTCGTGTGTTCGAGTCTTTGACGCATGATATACATTCCATTGTGTTTCCGTACTTTTCTCGGTGGCAAGCTGCCCATGGTTGGTGTTTTTTTTTTTTTTTTTTTTTTTATAATGAAATAAGTTGAGCTATGTTTAAATTGATATTGATAGTGATGTGTTTGTAATGATTATATATAGGAAAAATATTTACGTACTGGTTGGGAACGGAGCCGTTCTTGTACGTTGCAGAGCCGGAGTTTGTGAAGATGTTGTCGAGGGAAGTGCAAGCTAAGTACTGGGGGAAGCCGTCGGTGTTCAAACGTGATAGGAAATCGATGTTTGGGAATGGATTGGTGATGGCTGAAGGAGACGAGTGGGTTCGACAAAGACACGTCATCACTCCTGCATTTAATCCTTCCAACTTGAAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTCTCAGTTAAAAGGCTACTTAGTTTATATTTGTGAAAAAAAAAAAAAAAAAAAAACTCCTAAATTAAATTTTTTAAATTTTGAAAAATATTTTTAAAAGGGTTTTGAAAATTTTCTTATAAACTTTTAAAAATAGTATAAACATATTTGTAAAAGAATTTAATTTTTTTTCATTAATATATATGAACGAAGTCCTCTATTTACATTTGAGTATTAAAAGCAAAAAAATTATAAAAAAAAAAATTAGAAGTATTAATGTAATTATTGAAAGTTTGGTTTTTCTTTTAATTGAAAAGAAAAAGTTTAAAGATATTTTAAATTTAAAAAAAAAAAAACTAAAAATATTAATGAATTTTTTTAAAAGTTAAAAAGATATTTTTGAAACAAAATATTAATTATTTTTATATAAAATTGAGATATTTAAAAAAAAAATTAAGTTTAAGGGTATTTTTTAACATTTTTTCTAAATTTTAATCATATTGTTGAATTTTTTAAAAAAATTCATTGGTATTTAGAAGACAAAATACAAAATTGGATGATATTTGGATTAATTTAACCCCATATATATATATAATTTAAAACATGTCACATTCATACATTACATTAGTAGTGTCAAAAGAACATGAAACATATAGACATGCAAACATTGATTGCCTTTCAGCATGTATTCAATTCTAAGTTTTTTTTTTATTTTTTGACCATTTCAAAGTATATATTATTATCAATTCCTTTTCAAAAAAATAATAATTAGGAATGACCAACACGCAATCCGTACAGGCAATGGCAAGCTTAATGGTGGAGTCCACCACCCAAATGCTGGACAGGTGGGCCCACCTCGTACTTTCCGGCCATCCACAAATCGAAGTTGAAAACGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTTGGAATCGATCATATAAGCGGCAGACAAGTCTTCCACAAACTCAGGAATCTCCAGATGACACTCTTTAAAACCAACCGTCTTGTCGGAGTTCCGTTCGCCGGGGTTTTGAACGCCGGCAAGGCCCGAGAGGCCAAACGCCTTGGGGAAGAGATCGACGAGCTGTTTCGGGAGGTGATTACGGCGAGGAGAGAGAATCAGGCAGCGGCGGAAGTGGTGCAGCAAAATGATCTTTTGAGCTTGCTTTTGAAAGAGAGTGGCGGAGAGGGGAAGTTGGGGAGGTGGTTGAGTACGACGGAGCTGATTGATGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGCTGCTTTTGGGTATTCATACGGAGTGGCAAACTCAGCTGAGAGATGAGATTAAGGAAGTTCTTGGTGATAAGGAGAGTGACTTTGATTTCACTAAGCTTTCTCAGCTCAAAAAGGTATAAAAATTAAACCACGTTTTCATTAATCATAAAAGACATCAAAAAATCTGACACAATAAACAAAATGCAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCGTCCGCACCCAACGTGCAAAGGCAAGCTAGAAGAGACATTACAGTAAACGGACTGACCATCCCGAACGGCACCAACATGTGGATAGATGTGGTGGCCATGCATCACGACGAGGCCCTTTGGGGCGAGCAGGTGAACGAGTTTCGGCCGGAGAGATTCGAACACGATGGGGTAGCTGGGGGGTGCAGCCACAAGATGGGGTATTTGCCGTTTGGGTTTGGAGGAAGAATGTGCGTTGGAAGGCACTTGACGTTCATGGAGTATAAGATTGTGTTGACGCTTATTTTGTGTAGGTTTTCATTCACATTGTCTCCTGATTACTGCCATTCGCCTTGCATTATGCTGTCGCTCCGCCCTGCGCATGGTCTACCTCTCATTTTTCAACTACTGGAACACTAA

mRNA sequence

ATGGCGTTCTCTACTTCCTTGCTCTTCATGCTATTGTTTTCTTGTTGGATTTCACCTTTTTTAGCCCACAAAAAGCTAAAGCGAAATGGGTTTCGAGGTCCGACCCCGAGTTTTCCTCTCGGGAATATTAGCGAGATGAAAAGGACGAGCTTTGGTCGTGTGTTCGAGTCTTTGACGCATGATATACATTCCATTGTGTTTCCGTACTTTTCTCGGTGGCAAGCTGCCCATGAGCCGGAGTTTGTGAAGATGTTGTCGAGGGAAGTGCAAGCTAAGTACTGGGGGAAGCCGTCGGTGTTCAAACGTGATAGGAAATCGATGTTTGGGAATGGATTGGTGATGGCTGAAGGAGACGAGTGGGTTCGACAAAGACACGTCATCACTCCTGCATTTAATCCTTCCAACTTGAAGGCAATGGCAAGCTTAATGGTGGAGTCCACCACCCAAATGCTGGACAGGTGGGCCCACCTCGTACTTTCCGGCCATCCACAAATCGAAGTTGAAAACGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTTGGAATCGATCATATAAGCGGCAGACAAGTCTTCCACAAACTCAGGAATCTCCAGATGACACTCTTTAAAACCAACCGTCTTGTCGGAGTTCCGTTCGCCGGGGTTTTGAACGCCGGCAAGGCCCGAGAGGCCAAACGCCTTGGGGAAGAGATCGACGAGCTGTTTCGGGAGGTGATTACGGCGAGGAGAGAGAATCAGGCAGCGGCGGAAGTGGTGCAGCAAAATGATCTTTTGAGCTTGCTTTTGAAAGAGAGTGGCGGAGAGGGGAAGTTGGGGAGGTGGTTGAGTACGACGGAGCTGATTGATGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGCTGCTTTTGGGTATTCATACGGAGTGGCAAACTCAGCTGAGAGATGAGATTAAGGAAGTTCTTGGTGATAAGGAGAGTGACTTTGATTTCACTAAGCTTTCTCAGCTCAAAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCGTCCGCACCCAACGTGCAAAGGCAAGCTAGAAGAGACATTACAGTAAACGGACTGACCATCCCGAACGGCACCAACATGTGGATAGATGTGGTGGCCATGCATCACGACGAGGCCCTTTGGGGCGAGCAGGTGAACGAGTTTCGGCCGGAGAGATTCGAACACGATGGGGTAGCTGGGGGGTGCAGCCACAAGATGGGGTATTTGCCGTTTGGGTTTGGAGGAAGAATGTGCGTTGGAAGGCACTTGACGTTCATGGAGTATAAGATTGTGTTGACGCTTATTTTGTGTAGGTTTTCATTCACATTGTCTCCTGATTACTGCCATTCGCCTTGCATTATGCTGTCGCTCCGCCCTGCGCATGGTCTACCTCTCATTTTTCAACTACTGGAACACTAA

Coding sequence (CDS)

ATGGCGTTCTCTACTTCCTTGCTCTTCATGCTATTGTTTTCTTGTTGGATTTCACCTTTTTTAGCCCACAAAAAGCTAAAGCGAAATGGGTTTCGAGGTCCGACCCCGAGTTTTCCTCTCGGGAATATTAGCGAGATGAAAAGGACGAGCTTTGGTCGTGTGTTCGAGTCTTTGACGCATGATATACATTCCATTGTGTTTCCGTACTTTTCTCGGTGGCAAGCTGCCCATGAGCCGGAGTTTGTGAAGATGTTGTCGAGGGAAGTGCAAGCTAAGTACTGGGGGAAGCCGTCGGTGTTCAAACGTGATAGGAAATCGATGTTTGGGAATGGATTGGTGATGGCTGAAGGAGACGAGTGGGTTCGACAAAGACACGTCATCACTCCTGCATTTAATCCTTCCAACTTGAAGGCAATGGCAAGCTTAATGGTGGAGTCCACCACCCAAATGCTGGACAGGTGGGCCCACCTCGTACTTTCCGGCCATCCACAAATCGAAGTTGAAAACGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTTGGAATCGATCATATAAGCGGCAGACAAGTCTTCCACAAACTCAGGAATCTCCAGATGACACTCTTTAAAACCAACCGTCTTGTCGGAGTTCCGTTCGCCGGGGTTTTGAACGCCGGCAAGGCCCGAGAGGCCAAACGCCTTGGGGAAGAGATCGACGAGCTGTTTCGGGAGGTGATTACGGCGAGGAGAGAGAATCAGGCAGCGGCGGAAGTGGTGCAGCAAAATGATCTTTTGAGCTTGCTTTTGAAAGAGAGTGGCGGAGAGGGGAAGTTGGGGAGGTGGTTGAGTACGACGGAGCTGATTGATGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGCTGCTTTTGGGTATTCATACGGAGTGGCAAACTCAGCTGAGAGATGAGATTAAGGAAGTTCTTGGTGATAAGGAGAGTGACTTTGATTTCACTAAGCTTTCTCAGCTCAAAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCGTCCGCACCCAACGTGCAAAGGCAAGCTAGAAGAGACATTACAGTAAACGGACTGACCATCCCGAACGGCACCAACATGTGGATAGATGTGGTGGCCATGCATCACGACGAGGCCCTTTGGGGCGAGCAGGTGAACGAGTTTCGGCCGGAGAGATTCGAACACGATGGGGTAGCTGGGGGGTGCAGCCACAAGATGGGGTATTTGCCGTTTGGGTTTGGAGGAAGAATGTGCGTTGGAAGGCACTTGACGTTCATGGAGTATAAGATTGTGTTGACGCTTATTTTGTGTAGGTTTTCATTCACATTGTCTCCTGATTACTGCCATTCGCCTTGCATTATGCTGTCGCTCCGCCCTGCGCATGGTCTACCTCTCATTTTTCAACTACTGGAACACTAA

Protein sequence

MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTHDIHSIVFPYFSRWQAAHEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLEH
Homology
BLAST of Carg08409 vs. NCBI nr
Match: KAG7035636.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 998.8 bits (2581), Expect = 1.6e-287
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH
Sbjct: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60

Query: 61  DIHSIVFPYFSRWQAAHEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEW 120
           DIHSIVFPYFSRWQAAHEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEW
Sbjct: 61  DIHSIVFPYFSRWQAAHEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEW 120

Query: 121 VRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIA 180
           VRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIA
Sbjct: 121 VRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIA 180

Query: 181 KTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFR 240
           KTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFR
Sbjct: 181 KTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFR 240

Query: 241 EVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTA 300
           EVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTA
Sbjct: 241 EVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTA 300

Query: 301 LALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSA 360
           LALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSA
Sbjct: 301 LALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSA 360

Query: 361 PNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCS 420
           PNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCS
Sbjct: 361 PNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCS 420

Query: 421 HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGL 480
           HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGL
Sbjct: 421 HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGL 480

Query: 481 PLIFQLLEH 490
           PLIFQLLEH
Sbjct: 481 PLIFQLLEH 489

BLAST of Carg08409 vs. NCBI nr
Match: XP_022958036.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 979.5 bits (2531), Expect = 1.0e-281
Identity = 484/506 (95.65%), Postives = 486/506 (96.05%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF TSLLF +LFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSF RVFESLTH
Sbjct: 12  MAFLTSLLFKILFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFDRVFESLTH 71

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAAH                 EPEFVKMLSREVQAKYWGKPSVFKRD
Sbjct: 72  DIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKPSVFKRD 131

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP
Sbjct: 132 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 191

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAG+LNAG
Sbjct: 192 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGILNAG 251

Query: 241 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 300
           KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE
Sbjct: 252 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 311

Query: 301 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 360
           LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL
Sbjct: 312 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 371

Query: 361 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 420
           KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN
Sbjct: 372 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 431

Query: 421 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 480
           EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD
Sbjct: 432 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 491

Query: 481 YCHSPCIMLSLRPAHGLPLIFQLLEH 490
           YCHSPCIMLSLRPAHGLPLIFQLLEH
Sbjct: 492 YCHSPCIMLSLRPAHGLPLIFQLLEH 517

BLAST of Carg08409 vs. NCBI nr
Match: XP_023533013.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974.9 bits (2519), Expect = 2.5e-280
Identity = 485/510 (95.10%), Postives = 487/510 (95.49%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF TSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH
Sbjct: 1   MAFFTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAAH                 EPEFV+MLSREVQAKYWGKPSVFKRD
Sbjct: 61  DIHSIVFPYFSRWQAAHGKIFTYWLGTEPFLYIAEPEFVRMLSREVQAKYWGKPSVFKRD 120

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP
Sbjct: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAG+L AG
Sbjct: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGILIAG 240

Query: 241 KAREAKRLGEEIDELFREVITARRENQ----AAAEVVQQNDLLSLLLKESGGEGKLGRWL 300
           KAREAKRLGEEIDELFREVITARRENQ    AAAEVVQQNDLLSLLLKESGGEGKLGRWL
Sbjct: 241 KAREAKRLGEEIDELFREVITARRENQAAAAAAAEVVQQNDLLSLLLKESGGEGKLGRWL 300

Query: 301 STTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTK 360
           STTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTK
Sbjct: 301 STTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTK 360

Query: 361 LSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWG 420
           LSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWG
Sbjct: 361 LSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWG 420

Query: 421 EQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFT 480
           EQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFT
Sbjct: 421 EQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFT 480

Query: 481 LSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 490
           LSPDYCHSPCIMLSLRPAHGLPLIFQLLEH
Sbjct: 481 LSPDYCHSPCIMLSLRPAHGLPLIFQLLEH 510

BLAST of Carg08409 vs. NCBI nr
Match: XP_022995689.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 951.4 bits (2458), Expect = 3.0e-273
Identity = 474/509 (93.12%), Postives = 480/509 (94.30%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF  SLLF +LFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFE LTH
Sbjct: 12  MAFFISLLFKVLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFECLTH 71

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAA+                 +PEFVKMLSREVQAKYWGKPSVFKRD
Sbjct: 72  DIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKPSVFKRD 131

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP
Sbjct: 132 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 191

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAG+LNAG
Sbjct: 192 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGILNAG 251

Query: 241 KAREAKRLGEEIDELFREVITARRENQ---AAAEVVQQNDLLSLLLKESGGEGKLGRWLS 300
           KAREAKRLGEEIDELFREVITARRENQ   AAAEVV QNDLLSLLLKESGGEGKL RWLS
Sbjct: 252 KAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGKLRRWLS 311

Query: 301 TTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKL 360
           T ELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQ QLRDEIKEVLGDKESDFDFTKL
Sbjct: 312 TAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESDFDFTKL 371

Query: 361 SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE 420
           SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE
Sbjct: 372 SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE 431

Query: 421 QVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL 480
           QVNEFRPERFEHD VAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL
Sbjct: 432 QVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL 491

Query: 481 SPDYCHSPCIMLSLRPAHGLPLIFQLLEH 490
           SPDYCHSPCIMLSLRPAHGLPLIF+LLE+
Sbjct: 492 SPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

BLAST of Carg08409 vs. NCBI nr
Match: KAG6605729.1 (Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.4 bits (2406), Expect = 3.2e-267
Identity = 467/506 (92.29%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF TSLLF+LLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH
Sbjct: 12  MAFFTSLLFVLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 71

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAAH                 EPEFVKMLSREVQAKYWGKPSVFKRD
Sbjct: 72  DIHSIVFPYFSRWQAAHGKIFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKPSVFKRD 131

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEW                 AMASLMVESTTQMLDRWA LVLSGHP
Sbjct: 132 RKSMFGNGLVMAEGDEW-----------------AMASLMVESTTQMLDRWAQLVLSGHP 191

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG
Sbjct: 192 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 251

Query: 241 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 300
           KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE
Sbjct: 252 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 311

Query: 301 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 360
           LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQ QLRDEIKEVLGDKESDFDFTKLSQL
Sbjct: 312 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESDFDFTKLSQL 371

Query: 361 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 420
           KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN
Sbjct: 372 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 431

Query: 421 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 480
           EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD
Sbjct: 432 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 491

Query: 481 YCHSPCIMLSLRPAHGLPLIFQLLEH 490
           YCHSPCIMLSLRPAHGLPLIF+LLEH
Sbjct: 492 YCHSPCIMLSLRPAHGLPLIFRLLEH 500

BLAST of Carg08409 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 6.1e-80
Identity = 177/514 (34.44%), Postives = 271/514 (52.72%), Query Frame = 0

Query: 7   LLFMLLFS-----CWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKR----TSFGRVFES 66
           +L +LLFS      W+ P    +KL+  G RGP PSF  GNI EM+R           E+
Sbjct: 17  ILCILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEA 76

Query: 67  LTHDIHS-----IVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWG 126
            + D+ S      +FPYF  W   +                 +P  VK L+   ++   G
Sbjct: 77  GSTDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELA-NCKSLDLG 136

Query: 127 KPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWA 186
           KP   +++R ++ G G++ + GD WV QR VI P      +K M +LM+E+   ML+ W 
Sbjct: 137 KPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWK 196

Query: 187 HLV--LSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVG 246
           + V    G  +I V+  + + + ++I++  FG     G+++F K+R LQ  + K + L+G
Sbjct: 197 NEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIG 256

Query: 247 VPFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEG 306
           VP +  L     R    L   I  L    I+ + E+ ++  V    DLL  +++ S  +G
Sbjct: 257 VPGSRYLPTRSNRGIWNLDSSIRTLILN-ISKKYEHDSSTSV--NKDLLHSIIQGS-KDG 316

Query: 307 KLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKES 366
                     ++D CK  +F GHETT+    W L+LL  H EWQ++ R E  ++   +  
Sbjct: 317 PFASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPL 376

Query: 367 DFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHH 426
           DFD   L +LKK+  V+ E LRLYP A  V R+A  D+ + G+ IP GTN+WI +   H 
Sbjct: 377 DFDI--LRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHR 436

Query: 427 DEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 486
           D ++WG   ++F P+RF  +G+AG C     Y+PFG G R C G++L  +E K+VL+L+L
Sbjct: 437 DPSVWGPSADKFDPDRFA-NGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLL 496

Query: 487 CRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLL 488
            +F F LSP+Y H P   L++ P  G+PLIF+ L
Sbjct: 497 SKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Carg08409 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 6.4e-77
Identity = 176/509 (34.58%), Postives = 277/509 (54.42%), Query Frame = 0

Query: 3   FSTSLLFML--LFSC-WISPFLAHKKLKRNGFRGPTPSFPLGN---ISEMKRTSFGRVFE 62
           F T++L +L    SC W++P    K +++ G  GP P    GN   IS M   S  +  +
Sbjct: 13  FVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCD 72

Query: 63  SLTHDIHSIVFPYFSRWQAAHEPEFV-------------KMLSREVQAKY---WGKPSVF 122
           S+ HDI   + P++  W   +   F+               L +E+  K+    G+  + 
Sbjct: 73  SIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQ 132

Query: 123 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 182
           ++  K+  G GL+MA G +W  QRH+  PAF    LK  A  MVE T+++++R    V  
Sbjct: 133 QQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGE 192

Query: 183 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVL 242
           G  ++E+  E+     +II++T FG     G+++F+ L  LQ    +  R +  P +  L
Sbjct: 193 GANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFL 252

Query: 243 NAGKAREAKRLGEEIDELFREVITARRE-NQAAAEVVQQNDLLSLLLKESGGEGKLGRWL 302
            +   RE K L +E++ L  E+I +RR+  +        +DLL LLL E   +       
Sbjct: 253 PSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNN 312

Query: 303 STTELI-DECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFT 362
           +  +LI DECKTFFF GHETTAL LTWT +LL  +  WQ ++R+E++EV G +       
Sbjct: 313 NNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFG-RNGLPSVD 372

Query: 363 KLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALW 422
           +LS+L  +  V++E LRLYP A  + R A  D+ +  LTIP G ++WI V+A+HH E LW
Sbjct: 373 QLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELW 432

Query: 423 GEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSF 482
           G+  N+F PERF     A G      ++PF  G R C+G+    ME KI+L  ++ +F+F
Sbjct: 433 GKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNF 492

Query: 483 TLSPDYCHSPCIMLSLRPAHGLPLIFQLL 488
           T+S +Y H+P ++L+++P +G+ +I + L
Sbjct: 493 TISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Carg08409 vs. ExPASy Swiss-Prot
Match: B9G934 (Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-73
Identity = 166/500 (33.20%), Postives = 260/500 (52.00%), Query Frame = 0

Query: 8   LFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMK--RTSFGRVFESLTHDIHSI 67
           LF L    W+      KKL+R G RGP P+F  GN  E+K  R       +  T++  S 
Sbjct: 19  LFYLCNILWLRAVKIRKKLRRQGIRGPKPTFLYGNTKEIKRIRQELKLSQKQGTNNFIST 78

Query: 68  VFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRDRKSMF 127
           +FP+F  W+  +                  P+ VK + R   ++  GKP+  K+ RK++F
Sbjct: 79  LFPHFLLWRETYGPVFLYSTGAMEILQVSHPDMVKDIGRWTPSEL-GKPNYLKKSRKALF 138

Query: 128 GNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLV--LSGHPQIE 187
           G GL    GDEW  QR +I P F    +K M  L+ ++T  +L+ W  ++    G  +I 
Sbjct: 139 GGGLFTENGDEWAYQRKIIAPEFFMDKIKGMIQLIEDATVPVLEAWEDMIDDEGGCREIV 198

Query: 188 VENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGV-PFAGVLNAGKA 247
           V++ + + + ++IA+  FG     G ++F KLR LQ  + + +  VG+      L    +
Sbjct: 199 VDDYLRNLSADVIARACFGSSFTKGEEIFCKLRQLQKAIARQDSFVGLSALWKYLPTKSS 258

Query: 248 REAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE-- 307
           +E + L E++  L  +V   +   Q +      N L++ ++   G +   GR  +  E  
Sbjct: 259 QEIQMLDEQVRLLILDVAKEQHHYQDS-----HNSLVNAII--DGAQD--GRSAAEAEDF 318

Query: 308 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 367
           ++  CKT +FGGHE+TA+   W L+LL  H EWQ + R E  EV   + S  D   L +L
Sbjct: 319 IVGNCKTIYFGGHESTAVTAIWCLMLLATHPEWQERARAEAMEVCRGR-STLDVDALRRL 378

Query: 368 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 427
           K +  V+ E LRLYP A  + R+A  D+ +  + +P GT + +  + +H D+  WG   +
Sbjct: 379 KIVTMVIQETLRLYPPASVMMREALTDVKLGSIEVPRGTIVQVPRLMLHLDKEAWGADAD 438

Query: 428 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 484
           EFRP+RF  +GVA  C     Y+PFG G R C+G++L   E K+VL  +L +F+F+ SP 
Sbjct: 439 EFRPDRFA-NGVAAACRAAHMYVPFGHGPRTCIGQNLAMAELKVVLARLLTKFAFSPSPR 498

BLAST of Carg08409 vs. ExPASy Swiss-Prot
Match: Q7XHW5 (Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 3.3e-73
Identity = 167/516 (32.36%), Postives = 262/516 (50.78%), Query Frame = 0

Query: 11  LLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEM----------KRTSFGRVFESLTH 70
           L +  W++P      L+R G  GPTPSFP GN+++M          K T  GR    + H
Sbjct: 22  LYYVLWLAPERLRAHLRRQGIGGPTPSFPYGNLADMRSHAAAAAGGKATGEGRQEGDIVH 81

Query: 71  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 130
           D    VFP++  W+  +                  P+ V+ LS  V     GK S  K  
Sbjct: 82  DYRQAVFPFYENWRKQYGPVFTYSVGNMVFLHVSRPDIVRELSLCVSLDL-GKSSYMKAT 141

Query: 131 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLV-LSGH 190
            + +FG G++ + G+ W  QR +I P F P  +K M  LMV+S   ++  W   +  SG 
Sbjct: 142 HQPLFGEGILKSNGNAWAHQRKLIAPEFFPDKVKGMVDLMVDSAQVLVSSWEDRIDRSGG 201

Query: 191 PQIE--VENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLF--KTN---RLVGVP 250
             ++  ++++I + + ++I++T FG  ++ G+Q+F  +R LQ T+   K N    + G+ 
Sbjct: 202 NALDLMIDDDIRAYSADVISRTCFGSSYVKGKQIFDMIRELQKTVSTKKQNLLAEMTGLS 261

Query: 251 FAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKES-GGEGK 310
           F     +G+A  A RL   +  L  +++    E           +LLS +L+ + GG G 
Sbjct: 262 FLFPKASGRA--AWRLNGRVRALILDLVGENGEEDG-------GNLLSAMLRSARGGGGG 321

Query: 311 LGRWLSTTE--LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEV-LGDK 370
            G   +  E  ++D CK  +F G+E+TA+   W L+LL +H EWQ ++RDE++    G  
Sbjct: 322 GGEVAAAAEDFVVDNCKNIYFAGYESTAVTAAWCLMLLALHPEWQDRVRDEVQAACCGGG 381

Query: 371 ESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAM 430
               DF  L ++K +  V+ E LRLYP+   V RQA R++++ G+ +P G N+++ V  +
Sbjct: 382 GRSPDFPALQKMKNLTMVIQETLRLYPAGAVVSRQALRELSLGGVRVPRGVNIYVPVSTL 441

Query: 431 HHDEALWGEQVN--EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVL 486
           H D  LWG      EF P RF     A        YLPFG G R C+G+     E K++L
Sbjct: 442 HLDAELWGGGAGAAEFDPARF-----ADARPPLHAYLPFGAGARTCLGQTFAMAELKVLL 501

BLAST of Carg08409 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 9.5e-73
Identity = 170/534 (31.84%), Postives = 268/534 (50.19%), Query Frame = 0

Query: 10  MLLFSCWIS-PFLAHKKLKRNGFRGPTP-SFPLGNISEMKRT------------------ 69
           + + + W++ P    +  +R G  GP P SF  GN+ EMK                    
Sbjct: 25  LCITAAWLARPRRVAEVFRRQGIDGPPPSSFLAGNLPEMKARVAAAASAAAPTADGEETA 84

Query: 70  -----SFGRVFESLTHDIH-SIVFPYFSRWQAAH-----------------EPEFVKMLS 129
                  GR FE    D + + +FPYF +W+ A+                 +PE +  + 
Sbjct: 85  SAGGGGGGRDFEKDGFDDYCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIG 144

Query: 130 REVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVES 189
           R V     GKP   ++ ++ +FG G++ A G  W RQR VI P F  + ++AM  LMV++
Sbjct: 145 RCVSLD-MGKPKYLQKGQEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDA 204

Query: 190 TTQMLDRWAHLV----LSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQ 249
              ++  W   +     +   ++ V+ ++ S + ++I++  FG D+  GR++F +LR L 
Sbjct: 205 AQPLIASWESRIDAAGGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELS 264

Query: 250 MTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFREVITARR---------ENQAAA 309
             + +T+ +  +P    L  GK R   RL  EI  L  E++  RR           +AA 
Sbjct: 265 GLMSETSVIFSIPSLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAP 324

Query: 310 EVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIH 369
               + D L  +++ SGG+ +   +     ++D CK  +F GHET+A+  TW L+LL  H
Sbjct: 325 PSPPERDFLGSIIENSGGQPRPDDF-----VVDNCKNIYFAGHETSAVTATWCLMLLAAH 384

Query: 370 TEWQTQLRDEIKEVLGDKESDF----DFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARR 429
            EWQ + R E+ EV G   +      DF  +S+++ +G V+ E LRL+P +  V R+  R
Sbjct: 385 PEWQDRARAEVLEVCGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFR 444

Query: 430 DITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSH-KMGYLPF 483
           D+ +  L  P GT +++ V  MHHD A WG     F P RF  DGVA  C H +  ++PF
Sbjct: 445 DMQLGRLLAPKGTYLFVPVSTMHHDVAAWGPTARLFDPSRF-RDGVAAACKHPQASFMPF 504

BLAST of Carg08409 vs. ExPASy TrEMBL
Match: A0A6J1H0W5 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 4.9e-282
Identity = 484/506 (95.65%), Postives = 486/506 (96.05%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF TSLLF +LFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSF RVFESLTH
Sbjct: 12  MAFLTSLLFKILFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFDRVFESLTH 71

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAAH                 EPEFVKMLSREVQAKYWGKPSVFKRD
Sbjct: 72  DIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKPSVFKRD 131

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP
Sbjct: 132 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 191

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAG+LNAG
Sbjct: 192 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGILNAG 251

Query: 241 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 300
           KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE
Sbjct: 252 KAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE 311

Query: 301 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 360
           LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL
Sbjct: 312 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQL 371

Query: 361 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 420
           KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN
Sbjct: 372 KKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVN 431

Query: 421 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 480
           EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD
Sbjct: 432 EFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPD 491

Query: 481 YCHSPCIMLSLRPAHGLPLIFQLLEH 490
           YCHSPCIMLSLRPAHGLPLIFQLLEH
Sbjct: 492 YCHSPCIMLSLRPAHGLPLIFQLLEH 517

BLAST of Carg08409 vs. ExPASy TrEMBL
Match: A0A6J1K6M8 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.4e-273
Identity = 474/509 (93.12%), Postives = 480/509 (94.30%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFESLTH 60
           MAF  SLLF +LFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFE LTH
Sbjct: 12  MAFFISLLFKVLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFECLTH 71

Query: 61  DIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRD 120
           DIHSIVFPYFSRWQAA+                 +PEFVKMLSREVQAKYWGKPSVFKRD
Sbjct: 72  DIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKPSVFKRD 131

Query: 121 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 180
           RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP
Sbjct: 132 RKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP 191

Query: 181 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAG 240
           QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAG+LNAG
Sbjct: 192 QIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGILNAG 251

Query: 241 KAREAKRLGEEIDELFREVITARRENQ---AAAEVVQQNDLLSLLLKESGGEGKLGRWLS 300
           KAREAKRLGEEIDELFREVITARRENQ   AAAEVV QNDLLSLLLKESGGEGKL RWLS
Sbjct: 252 KAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGKLRRWLS 311

Query: 301 TTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKL 360
           T ELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQ QLRDEIKEVLGDKESDFDFTKL
Sbjct: 312 TAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESDFDFTKL 371

Query: 361 SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE 420
           SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE
Sbjct: 372 SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE 431

Query: 421 QVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL 480
           QVNEFRPERFEHD VAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL
Sbjct: 432 QVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL 491

Query: 481 SPDYCHSPCIMLSLRPAHGLPLIFQLLEH 490
           SPDYCHSPCIMLSLRPAHGLPLIF+LLE+
Sbjct: 492 SPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

BLAST of Carg08409 vs. ExPASy TrEMBL
Match: A0A1S4DS94 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 2.0e-219
Identity = 392/525 (74.67%), Postives = 433/525 (82.48%), Query Frame = 0

Query: 3   FSTSLLFML--LFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMK------------- 62
           F   +LF+L  LF CW+SPFL HKKLKRNGFRGPTP FPLGNISEMK             
Sbjct: 14  FGLFILFVLRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKMKMKMKNVNNPNS 73

Query: 63  --RTSFGRVFESLTHDIHSIVFPYFSRWQAAH-----------------EPEFVKMLSRE 122
               SF    + +TH+IHS VFPYF+RWQ  +                 +PEFVK+LS+E
Sbjct: 74  TFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIADPEFVKILSKE 133

Query: 123 VQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTT 182
           V+AK WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLKAMASLMVES T
Sbjct: 134 VKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESAT 193

Query: 183 QMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKT 242
           +M++RWA+LV+SG+PQIEVE EITSTAGEIIAKTSFGI H SGRQVF+KLR LQ+TLFKT
Sbjct: 194 KMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQITLFKT 253

Query: 243 NRLVGVPFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVV--QQNDLLSLLL 302
           NRLVGVPF G+LNA KAREAK LGEEIDELFR VIT RRE+  A      QQNDLLSLLL
Sbjct: 254 NRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTTEQQNDLLSLLL 313

Query: 303 KES--GGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEI 362
           KES  GG G+ GR LST ELIDECKTFFFGGHETTALALTWTL+LL +H+EWQT LR+EI
Sbjct: 314 KESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEI 373

Query: 363 KEVLG-DKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTN 422
           KEV G + ++ FDFT LS LKKMGWVMSEVLRLYPSAPNVQRQAR+DI +NGLTIPNGTN
Sbjct: 374 KEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIIINGLTIPNGTN 433

Query: 423 MWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFM 482
           MWIDVV+MHHD+ALWG QVNEF PERF +D V+GGC+HKMGYLPFGFGGRMCVGRHLTFM
Sbjct: 434 MWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFM 493

Query: 483 EYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 489
           EYKIVLTLIL RFSF+LSPDY HSP IMLSLRPAHGLPL+F+LLE
Sbjct: 494 EYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLE 538

BLAST of Carg08409 vs. ExPASy TrEMBL
Match: A0A6J1E0B2 (cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.5e-219
Identity = 391/511 (76.52%), Postives = 432/511 (84.54%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFG-RVFES-- 60
           M+    L+  +LF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ +    V ES  
Sbjct: 11  MSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQ 70

Query: 61  LTHDIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVF 120
           +TH+IHS VFPYFSRWQA+H                 EPEF+K LS EV+AK+WGKPSVF
Sbjct: 71  VTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVF 130

Query: 121 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 180
           K DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWA ++ S
Sbjct: 131 KDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWARMINS 190

Query: 181 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVL 240
           G  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF  +L
Sbjct: 191 GDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFEKLRALQMTLFKTNRLVGVPFGRLL 250

Query: 241 NAGKAREAKRLGEEIDELFREVITARRE---NQAAAEVVQQNDLLSLLLKESGGEGKLGR 300
           NAGKAREA+RLGEEID LF  VITARRE   NQ       Q DLLSLLL++ G     GR
Sbjct: 251 NAGKAREAERLGEEIDGLFESVITARREGNNNQV------QQDLLSLLLRDDG-----GR 310

Query: 301 WLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDF 360
            LSTTEL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQTQLR+E+KEVLG+KES+FDF
Sbjct: 311 RLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKESEFDF 370

Query: 361 TKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEAL 420
           + L+ LKKMGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHDEAL
Sbjct: 371 SNLAGLKKMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEAL 430

Query: 421 WGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFS 480
           WG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILCRFS
Sbjct: 431 WGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFS 490

Query: 481 FTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 489
           F+LSP Y HSP IMLSLRPAHGLPLIFQLLE
Sbjct: 491 FSLSPAYRHSPSIMLSLRPAHGLPLIFQLLE 510

BLAST of Carg08409 vs. ExPASy TrEMBL
Match: E5GCU7 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.7e-213
Identity = 381/506 (75.30%), Postives = 419/506 (82.81%), Query Frame = 0

Query: 20  FLAHKKLKRNGFRGPTPSFPLGNISEMK---------------RTSFGRVFESLTHDIHS 79
           F  HKKLKRNGFRGPTP FPLGNISEMK                 SF    + +TH+IHS
Sbjct: 6   FEVHKKLKRNGFRGPTPCFPLGNISEMKMKMKMKNVNNPNSTFEDSFSHDDQLITHNIHS 65

Query: 80  IVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFKRDRKSM 139
            VFPYF+RWQ  +                 +PEFVK+LS+EV+AK WGKPSVFK DRKSM
Sbjct: 66  TVFPYFARWQTTYGKVFTYWLGTEPFLYIADPEFVKILSKEVKAKNWGKPSVFKNDRKSM 125

Query: 140 FGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHPQIEV 199
           FGNGLVMAEGDEWVRQRH+ITPAFNPSNLKAMASLMVES T+M++RWA+LV+SG+PQIEV
Sbjct: 126 FGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATKMVERWANLVISGNPQIEV 185

Query: 200 ENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLNAGKARE 259
           E EITSTAGEIIAKTSFGI H SGRQVF+KLR LQ+TLFKTNRLVGVPF G+LNA KARE
Sbjct: 186 EQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQITLFKTNRLVGVPFVGLLNAAKARE 245

Query: 260 AKRLGEEIDELFREVITARRENQAAAEVV--QQNDLLSLLLKES--GGEGKLGRWLSTTE 319
           AK LGEEIDELFR VIT RRE+  A      QQNDLLSLLLKES  GG G+ GR LST E
Sbjct: 246 AKLLGEEIDELFRSVITERREDAVAERTTTEQQNDLLSLLLKESSGGGGGEEGRRLSTAE 305

Query: 320 LIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLG-DKESDFDFTKLSQ 379
           LIDECKTFFFGGHETTALALTWTL+LL +H+EWQT LR+EIKEV G + ++ FDFT LS 
Sbjct: 306 LIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEVFGHNLQTHFDFTNLSS 365

Query: 380 LKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQV 439
           LKKMGWVMSEVLRLYPSAPNVQRQAR+DI +NGLTIPNGTNMWIDVV+MHHD+ALWG QV
Sbjct: 366 LKKMGWVMSEVLRLYPSAPNVQRQARKDIIINGLTIPNGTNMWIDVVSMHHDQALWGHQV 425

Query: 440 NEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSP 489
           NEF PERF +D V+GGC+HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL RFSF+LSP
Sbjct: 426 NEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSP 485

BLAST of Carg08409 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 549.7 bits (1415), Expect = 2.4e-156
Identity = 280/506 (55.34%), Postives = 352/506 (69.57%), Query Frame = 0

Query: 7   LLFMLLF-SCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFES-------L 66
           L+F+ LF  CWI P  A KKL+ NGF GP PSFP GN+++MK+     V          +
Sbjct: 23  LMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDNSKSSTII 82

Query: 67  THDIHSIVFPYFSRWQAAH-----------------EPEFVKMLSREVQAKYWGKPSVFK 126
            HDIHSI  P+F+RWQ  +                 +PEF+ ++S+ V  K WGKP+VFK
Sbjct: 83  NHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSWGKPNVFK 142

Query: 127 RDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSG 186
           +DR+ MFG GLVM EGD+W R RH+ITPAF P NLK M ++MVES + MLDRW   + SG
Sbjct: 143 KDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRWGIQINSG 202

Query: 187 HPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLN 246
           +P+ ++E+EI  TAGEIIAKTSFG+   +G QV   LR +Q  LF +NR VGVPF+ +L+
Sbjct: 203 NPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYVGVPFSNILS 262

Query: 247 AGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLST 306
             +  +AK LG EID L    I  R+ + A  +  Q +DLL +LLK +  +G      + 
Sbjct: 263 YKQTVKAKGLGHEIDGLLLSFINKRKISLAEGD-DQGHDLLGMLLK-ADQKGN----FTA 322

Query: 307 TELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLS 366
            EL+DECKTFFF GHETTALALTWT +LL IH EWQ  +R+EI+EV+GD  S  ++ KL+
Sbjct: 323 KELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGD--SKIEYNKLA 382

Query: 367 QLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQ 426
            LKKM WVM+EVLRLYP APN QRQAR DI VNG  IPNGTN+WIDVVAMHHD  LWG+ 
Sbjct: 383 GLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVELWGDD 442

Query: 427 VNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLS 486
           VNEF+PERF+   + GGC +KMGY+PFGFGGRMC+GR+LT MEYKIVL+L+L RF  ++S
Sbjct: 443 VNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFEISVS 502

Query: 487 PDYCHSPCIMLSLRPAHGLPLIFQLL 488
           P Y HSP  MLSLRP +GLPLI + L
Sbjct: 503 PGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of Carg08409 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-78
Identity = 176/509 (34.58%), Postives = 277/509 (54.42%), Query Frame = 0

Query: 3   FSTSLLFML--LFSC-WISPFLAHKKLKRNGFRGPTPSFPLGN---ISEMKRTSFGRVFE 62
           F T++L +L    SC W++P    K +++ G  GP P    GN   IS M   S  +  +
Sbjct: 13  FVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCD 72

Query: 63  SLTHDIHSIVFPYFSRWQAAHEPEFV-------------KMLSREVQAKY---WGKPSVF 122
           S+ HDI   + P++  W   +   F+               L +E+  K+    G+  + 
Sbjct: 73  SIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQ 132

Query: 123 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 182
           ++  K+  G GL+MA G +W  QRH+  PAF    LK  A  MVE T+++++R    V  
Sbjct: 133 QQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGE 192

Query: 183 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVL 242
           G  ++E+  E+     +II++T FG     G+++F+ L  LQ    +  R +  P +  L
Sbjct: 193 GANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFL 252

Query: 243 NAGKAREAKRLGEEIDELFREVITARRE-NQAAAEVVQQNDLLSLLLKESGGEGKLGRWL 302
            +   RE K L +E++ L  E+I +RR+  +        +DLL LLL E   +       
Sbjct: 253 PSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNN 312

Query: 303 STTELI-DECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFT 362
           +  +LI DECKTFFF GHETTAL LTWT +LL  +  WQ ++R+E++EV G +       
Sbjct: 313 NNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFG-RNGLPSVD 372

Query: 363 KLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALW 422
           +LS+L  +  V++E LRLYP A  + R A  D+ +  LTIP G ++WI V+A+HH E LW
Sbjct: 373 QLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELW 432

Query: 423 GEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSF 482
           G+  N+F PERF     A G      ++PF  G R C+G+    ME KI+L  ++ +F+F
Sbjct: 433 GKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNF 492

Query: 483 TLSPDYCHSPCIMLSLRPAHGLPLIFQLL 488
           T+S +Y H+P ++L+++P +G+ +I + L
Sbjct: 493 TISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Carg08409 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-73
Identity = 168/508 (33.07%), Postives = 263/508 (51.77%), Query Frame = 0

Query: 1   MAFSTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGN---ISEMKRTSFGRVFES 60
           M     +L+  +   +++P    K ++R G  GP P    GN   IS+M   S      S
Sbjct: 15  MTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSS 74

Query: 61  LTHDIHSIVFPYFSRWQAAHEPEFVKM-------------LSREVQAKY---WGKPSVFK 120
           + H+I   + P++  W   +   F+               + +E+  K+    GK  + +
Sbjct: 75  IHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQ 134

Query: 121 RDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSG 180
           +  K   G GL+MA G+ W  QRH+  PAF    LK  A  MVE T  M +R    V   
Sbjct: 135 QGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKEV--- 194

Query: 181 HPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGVLN 240
             ++E+  E+     +II++T FG     G+++F  L  LQ    +  R +  P +  L 
Sbjct: 195 GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLP 254

Query: 241 AGKAREAKRLGEEIDELFREVITARREN-QAAAEVVQQNDLLSLLLKESGGEGKLGRWLS 300
           +   RE K L  E++ L  E+I +R+++ +        +DLL LLL +          L+
Sbjct: 255 SKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKNN---LN 314

Query: 301 TTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKL 360
              ++DECKTFFF GHETT+L LTWTL+LL  +  WQ  +RDE+++V G ++      +L
Sbjct: 315 VQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCG-QDGVPSVEQL 374

Query: 361 SQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGE 420
           S L  +  V++E LRLYP A  + R A  DI +  L IP G ++WI V+A+HH   LWGE
Sbjct: 375 SSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGE 434

Query: 421 QVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTL 480
             NEF PERF     A        ++PF  G R C+G+    ME KI+L +++ +FSF +
Sbjct: 435 DANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAI 494

Query: 481 SPDYCHSPCIMLSLRPAHGLPLIFQLLE 489
           S +Y H+P ++L+++P +G+ L+ + L+
Sbjct: 495 SENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Carg08409 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 274.6 bits (701), Expect = 1.5e-73
Identity = 163/496 (32.86%), Postives = 252/496 (50.81%), Query Frame = 0

Query: 24  KKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFES--------LTHDIHSIVFPYFSRWQA 83
           +KL   G +GP PS   GN+ EM++     +  S        + HD  S +FPY   W+ 
Sbjct: 40  RKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMSNSKHYSGDNIIAHDYTSSLFPYLDHWRK 99

Query: 84  AH-----------------EPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGD 143
            +                  PE VK L+ +      GK S   +  KS+ G G++ + G 
Sbjct: 100 QYGRVYTYSTGVKQHLYMNHPELVKELN-QANTLNLGKVSYVTKRLKSILGRGVITSNGP 159

Query: 144 EWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP---QIEVENEITSTA 203
            W  QR +I P F    +K M  L+VES   ML +W  ++         I V+ ++ + +
Sbjct: 160 HWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEMVCDIIVDEDLRAAS 219

Query: 204 GEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGV-PFAGVLNAGKAREAKRLGEE 263
            ++I++  FG     G+++F KLR LQ  +   N L  +  F  V+   K    K    +
Sbjct: 220 ADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVVFGTK----KHGNGK 279

Query: 264 IDELFREV------ITARRENQAAAEVVQQNDLLSLLLK--ESGGEGKLGRWLSTTE--L 323
           IDEL R +          RE +   +   + DL+ L+L+   S  +G L     + +  +
Sbjct: 280 IDELERHIESLIWETVKERERECVGD--HKKDLMQLILEGARSSCDGNLEDKTQSYKSFV 339

Query: 324 IDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLK 383
           +D CK+ +F GHET+A+A++W L+LL ++  WQT++RDE+   L  K    D   +S LK
Sbjct: 340 VDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEV--FLHCKNGIPDADSISNLK 399

Query: 384 KMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNE 443
            +  V+ E LRLYP A  V R+A  D  +  L +P G  +W  +  +H D  +WG   NE
Sbjct: 400 TVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADANE 459

Query: 444 FRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDY 481
           F PERF  +GV+  C H   ++PFG G R+C+G++   ME K++++LI+ RFSFTLSP Y
Sbjct: 460 FNPERFS-EGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPTY 519

BLAST of Carg08409 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-72
Identity = 154/490 (31.43%), Postives = 248/490 (50.61%), Query Frame = 0

Query: 24  KKLKRNGFRGPTPSFPLGNISEMKRTSFGRVFES----LTHDIHSIVFPYFSRWQAAH-- 83
           + LK  G +GP PS   GN+SEM+R        S    ++HD  S +FP+F  W+  +  
Sbjct: 40  RSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKHCSGDNIISHDYSSSLFPHFDHWRKQYGR 99

Query: 84  ---------------EPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVR 143
                           PE VK LS +      G+ +   +    + GNG++ + G  W  
Sbjct: 100 IYTYSTGLKQHLYINHPEMVKELS-QTNTLNLGRITHITKRLNPILGNGIITSNGPHWAH 159

Query: 144 QRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLSGHP---QIEVENEITSTAGEII 203
           QR +I   F    +K M  LMVES   ML++W  +V  G      I V+ ++   + ++I
Sbjct: 160 QRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVI 219

Query: 204 AKTSFGIDHISGRQVFHKLRNL------QMTLFKTNRLVGVPFAGVLNAGKAREAKRLGE 263
           AK  FG     G+ +F  +R+L      +  LF+ N    + F    +     +A  +  
Sbjct: 220 AKACFGSSFSKGKAIFSMIRDLLTAITKRSVLFRFNGFTDMVFGSKKHGDVDIDALEM-- 279

Query: 264 EIDELFREVITARRENQAAAEVVQQNDLLSLLLKESGGEGKLGRWLSTTE---LIDECKT 323
              EL   +    +E +   +   + DL+ L+L+ +        W  +     ++D CK+
Sbjct: 280 ---ELESSIWETVKEREIECKDTHKKDLMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKS 339

Query: 324 FFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVM 383
            +F GH++TA++++W L+LL ++  WQ ++RDEI  +   K    D   +  LK +  V+
Sbjct: 340 IYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEI--LSSCKNGIPDAESIPNLKTVTMVI 399

Query: 384 SEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERF 443
            E +RLYP AP V R+A +DI +  L +P G  +W  + A+H D  +WG   N+F+PERF
Sbjct: 400 QETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF 459

Query: 444 EHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCI 481
             +G++  C +   Y+PFG G R CVG++   ME K++++LI+ +FSFTLSP Y HSP  
Sbjct: 460 S-EGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSH 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035636.11.6e-287100.00Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958036.11.0e-28195.65cytokinin hydroxylase-like [Cucurbita moschata][more]
XP_023533013.12.5e-28095.10cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022995689.13.0e-27393.12cytokinin hydroxylase-like [Cucurbita maxima][more]
KAG6605729.13.2e-26792.29Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q2QYH76.1e-8034.44Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q9FF186.4e-7734.58Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
B9G9341.9e-7333.20Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 ... [more]
Q7XHW53.3e-7332.36Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 ... [more]
Q5KQH79.5e-7331.84Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H0W54.9e-28295.65cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV... [more]
A0A6J1K6M81.4e-27393.12cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1[more]
A0A1S4DS942.0e-21974.67cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1[more]
A0A6J1E0B23.5e-21976.52cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 S... [more]
E5GCU71.7e-21375.30Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52400.12.4e-15655.34cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
AT5G38450.14.5e-7834.58cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.11.2e-7333.07cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G24910.11.5e-7332.86cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.11.3e-7231.43cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 221..248
NoneNo IPR availablePANTHERPTHR24282:SF149CYTOCHROME P450 FAMILY 72 PROTEINcoord: 3..483
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 3..483
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 303..329
score: 32.92
coord: 283..300
score: 27.5
coord: 425..435
score: 51.95
coord: 435..458
score: 32.66
IPR001128Cytochrome P450PRINTSPR00385P450coord: 294..311
score: 39.43
coord: 435..446
score: 34.73
coord: 348..359
score: 39.82
coord: 426..435
score: 57.77
IPR001128Cytochrome P450PFAMPF00067p450coord: 79..464
e-value: 7.3E-71
score: 239.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 19..486
e-value: 9.7E-105
score: 352.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 26..484
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 428..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08409-RACarg08409-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen