Carg08401 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08401
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionFAD-binding FR-type domain-containing protein
LocationCarg_Chr02: 5924802 .. 5929915 (-)
RNA-Seq ExpressionCarg08401
SyntenyCarg08401
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAGAGGAAAAAAGAAAAAAACAGGAACGGAGAAATTTATCTGATCAGTTATCATTTCATCATTAATGGCGTCCATGATGAAGAACCCAGCCATGTTCGCGCCTCATTTTCCATTGCTGCAACTGCCTTCATTTTGCCACCGTTCGCCTCTCCCTCTCCTCCGTGGCCCTCCCTCAGCTGCCGCTGCCGCTTCAGTATCGAATTCCTCATTTCGGAAAGGAAGCGGCGACAGAGAGCTCCTCTCACTTTCGTCTTCCTCGGGATACCGGAATTCGACTCGGTATGTGAAGGTTAGGTCACAGCTGAGGCATCCAATTATTGCGCCGGATGATTATTGGGGCACATGGACTGCCTTGTTTGCTATTGGCGCATTGGGGATCTGGTACTTTCTATCGATTCTTATTTGTTTAGCTCAAAGTGGATATCGATTGTGACTTGCATTTATTGCTAAGGAAAGCCACATTATAAGAACACATGGGATGTATTTTATGGTGTTGGAATGTGTTTGTTTTGGGGGAGGATTATAACAGGGAAAACACTAGTGAGACTTGTTTCTTTTTCTGTTCTGCCCTCAATAGTGGAAATTGAAGTTGTTCATGAATGGATTAGAAGTTTAGTTTTATTCTTGAATCATAATGTTAGCTGTAAGCATACTGCTTAATTGAATATGCCAATTATTCTTCAGATTTTTGTAACAGTGCTTCTTTTTACTCTTAAGTAAATCTTTCAAATGACCACAATGGTAGGAGGAAATGGCAAAAGGAAAAGAGGAAACTATGTGAACCAAGAGATATCAAACATATAATATACTTCAATGAAGATGTGAAGAAAATACTCTTAAAATTATCAAAAGATATTTCAATTCATGCCACCTGAAGAAGAATGAAGTTTTATTGTTATAAATTTTGGGTGGATTACAATTTAGAGTCTATTGTATTTCATTAATATATTTTAAGACTTTTTATTTACCTTAGGTTACATTTACATAATGGTTAATTATGACCATAAAATATGAATGTTACAACTCTTGGAAGGCCTTTAATAGTTTCATTTTGAGGCTATATAAAACCATGTATGTTGTATTTGTAAGCAGACTTGGAAAATATAGTAAAAAGGAGCATTAGTGCTTTCTTTAGCCAATGGTTAAGTTTCTGTGCAATTTTATGTAGTTTAGGTTGCATGTAGAATCATTCAAGCTTGATATGATCAATCTTGCTTGTGGAGTGATTTGAATCTCAAACAAGTGTTCTTGCCTTGAGATATTTGATCAACAAGGATCATTCAAGATAGACATGATCGATCTTGCTTGTGGAGTGATTCGAATCTCAAACAAGAGTTCTTGCTTTGAGATATTCAATCAACAAGCTAATCCAAATCTTACTTCCCTTGTAGTGATTTCCACATCATTTGGTATCAGAGCTCTTGGGCTGATTTTCTTATTATTTGGTATCAGAGCTCTTGGGCTGATTTCCTTATCATTTGGCATCTGACCTTTCCCTTGGGTTGGTTCCATATCATTGAAGGAAATTAAAGGGACGTTTGTTAGGATTGGACATTCTCGTTGCATGTTTAACGACCCATTAACGGTTTTGTTTGTTAGCTAATCTGGGATTACTCTGTGAATTTGGGTTTAAAGGTCTGAGAAGACCACAATAGGTAGAACAGTGAGTGCTGCTTTGGTTAGCACTTTGGTTGGCCTGGCAGCCAGTAACTTTGGCATAATCCCCTATGAAGCCGTGTCTTATTCAATTGTCATGGAAATTCTGCTTCCAGTATCTGTTGCCTTGCTACTATTTCGGGCAGACATGCGCCATATAATCCGATCAACTGGAACATTACTTGGAGTCTTCATGCTCGGATCAGGTAATTAATTTTTCTTCAGATTCTTGATAGCACTGACTTAGTAATTCAACCTATTTTCTTGAGAATTGGTTTGAATTTTGAAGCATTAATGATTTAGAGGTTCATGTTTCTAAATTAGTCGTGAATCTACTGTTTCTTAACTATTTCACCCGCTGTAATCTAATGCTAACTTCTTGCTGATTAAGCTGAATAATGAATAATTCTTTTTAATGGTCAATCTTGCTCTTTTTTGTATTGTTATTATCATTAATGTTTCCTTTTATATTTCAAAGTCCATGGCAACTTTGAATGATCATTTTTCTTACAAACCGAGAAGCTTGAAATCGAAATTTTTTTAAAAGCCAAATGGAATGTAGTAATAAAAAGCAATTTTTGAAAAATTGAAGTTTAGGGTCAGATATTTCATTCATGATCTAAAGCTGAAGGAAACGAACATAAATTAATAGGTCCGTTACAATTAATTGTTTGACAGCCTGATTTTCAGAATTGAAAGTTCAGAATAAATTTGCTACATTGTATTAAAATTGCGAGTTTGTTTTTAACTACTTTATTTTCTGTGGATGGCAGTTGCGACTATTATTGGAACTGTGGTAGCATTTCTCATGGTGCCTATGAGATCACTGGGTCCTGACAACTGGAAAATAGCTTCTGCTCTCATGGGTAGTTATATTGGTGGATGTAAGTTGTCAGAAAGTTTAAACCCTGTTATTACTGTAGTTATGTCTCAAGTGAGGCATTTATGACCGAAACGAGATTAGATGAAATTATCTTAGGACAACTTCTTTTCATTGAATGATATCAAAACCTGTCAGCATCATGTGGCCAAGCAACCCCATTAGTGGCAGAGATTATTGTTGTAAATCCAACCTCTCGCCCTTTATATTGATACAGAGACGTTTGGGGACCACTACCACATTGCTATTTCTTTCCCAGTTGGACAGTTTTATATTGTTACAGAGACGTTTAGAGCCTTATTGAAACTAGAAAATATCTAATCGAAGTGAAATTCAAACTCTTGACACTTGTTGATAAAATTAAAATTGCTCTTGTAGCCTTCTGGAAATTAATTTGAATAAAATATAGCTATCCACCTAGTGCAATAATGCAACGAAAAATGGCTATAATCCCCCACCCCCAGATTACTGTTGAGATATTCTATGTTGGAATACACTATAAAGTACTTTAAGGATGTCAAAGAAACGAGTCTATTAAATATTGATATGGCACAGCCATGATTGTCTATGGAGCACATTGTTGATTCATAAACGTTCATCACCCTACCAATATGTATCCAGTAAACAGAATTTGCGGCTATAGAAATAATTTTTCTTACCATGCTATCATTAGAGAGTCCTCTTGTGTCACTTGTAGAGGCTGAATTTTACTGGTCATCCATATCTATGTCTTCTGGAACTATGAAACATATACATCCTACTCATTCAGCATCCATTTTTCTCTATTTGATATTTCTGATAATGTGCCTGGTTAATGTGTCTCTACTCTTTTTTTCTTCTTTGACCTTTCTTCCTGGTTGATCTATGTAGCTGTTAATTATGTTGCAATTTCAGAGGCTCTTGGTGTTTCTCCATCAGTTTTAGCGGCAGGAGTGGCTGCTGACAATGTCATCAGTGCTTTATATTTTGTAGCCCTCTTTGCATTGGCTTCTAGAACACCTCCTGAACCCCCAACATCAACCAACGGTACATGGTTCATTCTAGCTTTAGTATCATGCTAATAATTTTCAGAAAATATGCATTTGGGTCTAGCATAAGAATTGGTGAAACCTCGGTTTTGAGCTGGAGTCCCTTTTGTTGTTGAGTTTGGACTCTTTTAGTGTGCTTTGTGTGTGTGTGTGTGTGTGTGTGTGTCCTCGTGTATTCTTTCATTGTGCTCAATGGAAGCTTAGTTTCTTGCCCAGAAAAAAGATGATGACAAACCATCCTACAGATGCACCATTAACCATGTGTGTAATTGTAACCCATGCTATTTATATCCTTTCAAGATTGAAAGTTTTTGGACAAAATAGTACCGTACAATTCCTGGTTTCCTACTACCACATCTATGATTTCTCAATTAAAATTTGAAAACTTTCCCAGATGCTTCAACTGATATGCACTCTGATAACGGCACGAAGCTCCCTGTGTTGCCGACTGCTACGGCTGTTTTTGCATCATTTGCAATATGCAAATTTGTCACGTGGATTACAAATATATGTAAAATTCAGGGTGCCAATCTTCCTGGGATAACTGCAGTTGTTGTCATTTTAGCTACCGTTTTCCCAAAACAGTTCAGTTATTTAGCACCCGCAGCTGATACCATTGCCCTGATTCTTATGCAGGTATGTATTTTTAGTTAGTTGCATCACTCACGGAAGCTTGTGCACCGTTTTCTATTCCTGCTCGTTATTAACCACAGAAAATTTACAGTAGTACTGTAGAGAGCACTTTGATTTTCCCGCCCAAAAGTTGTATGCTTTTGTTCTGTCTGGCCTATGGATTTGGCTCACCTTTAGTTATCTCCATTGCTTACTGAGAGGGTGGGGGAAGGCTTCAAGGAGCTGTTGCTTTGACTAGGTGAAAAAAGTACATATTGGGTGGGACCTGGAGTAAACAAATAAAAAACAGTAACGGTCGGCCATTATTACTTTATGAAAAACATGCATGTTGGGACCAAGTTTGTTCCCTAATGGCATGTCCTGAACACGATCCTGCTATTTTAGTTTGAGCTGATGAAAGAATAAATTTGAGCACAGGAAAGTAAGGGAAATGACACTATCCTTGTTTAAAAAATCATTTTAAATGTTCTAAACAAAAACTTCTAGAACTTGTAGATCTGGGTAAAACACAGCATGACATGAGATTTTTAAGTTAACTGAGGAACACCTGAAAGTTCAATTGGCTTGTTTATTTGATCTGACATGAACAGGTGTTCTTTGCGGTTGTCGGTGCTAGTGGGAGTGTGTGGTATGTCATCAACAATGCACCAAGTATTTTCATGTTTGCTCTAGTTCAAGTTACAGTCCATCTTCTTGTAATTCTTGGTTTTGGAAAGCTATTCAGCATTGATTTAAAGCTGTTGCTTCTGGCTTCAAATGCCAACATTGGAGGCCCAACAACAGCATGTGGAATGGCAACTGCCAAGGGTTGGAGATCTTTGGTGGTTCCTTCTATTCTTGCTGGCATTTTCGGTATTGCAATTGCTACTTTCCTGGGCATCAGTTTTGGGGTGATGATCCTCAGGCGTTTGTAG

mRNA sequence

AGAAAGAGGAAAAAAGAAAAAAACAGGAACGGAGAAATTTATCTGATCAGTTATCATTTCATCATTAATGGCGTCCATGATGAAGAACCCAGCCATGTTCGCGCCTCATTTTCCATTGCTGCAACTGCCTTCATTTTGCCACCGTTCGCCTCTCCCTCTCCTCCGTGGCCCTCCCTCAGCTGCCGCTGCCGCTTCAGTATCGAATTCCTCATTTCGGAAAGGAAGCGGCGACAGAGAGCTCCTCTCACTTTCGTCTTCCTCGGGATACCGGAATTCGACTCGGTATGTGAAGGTTAGGTCACAGCTGAGGCATCCAATTATTGCGCCGGATGATTATTGGGGCACATGGACTGCCTTGTTTGCTATTGGCGCATTGGGGATCTGGTCTGAGAAGACCACAATAGGTAGAACAGTGAGTGCTGCTTTGGTTAGCACTTTGGTTGGCCTGGCAGCCAGTAACTTTGGCATAATCCCCTATGAAGCCGTGTCTTATTCAATTGTCATGGAAATTCTGCTTCCAGTATCTGTTGCCTTGCTACTATTTCGGGCAGACATGCGCCATATAATCCGATCAACTGGAACATTACTTGGAGTCTTCATGCTCGGATCAGTTGCGACTATTATTGGAACTGTGGTAGCATTTCTCATGGTGCCTATGAGATCACTGGGTCCTGACAACTGGAAAATAGCTTCTGCTCTCATGGGTAGTTATATTGGTGGATCTGTTAATTATGTTGCAATTTCAGAGGCTCTTGGTGTTTCTCCATCAGTTTTAGCGGCAGGAGTGGCTGCTGACAATGTCATCAGTGCTTTATATTTTGTAGCCCTCTTTGCATTGGCTTCTAGAACACCTCCTGAACCCCCAACATCAACCAACGATGCTTCAACTGATATGCACTCTGATAACGGCACGAAGCTCCCTGTGTTGCCGACTGCTACGGCTGTTTTTGCATCATTTGCAATATGCAAATTTGTCACGTGGATTACAAATATATGTAAAATTCAGGGTGCCAATCTTCCTGGGATAACTGCAGTTGTTGTCATTTTAGCTACCGTTTTCCCAAAACAGTTCAGTTATTTAGCACCCGCAGCTGATACCATTGCCCTGATTCTTATGCAGGTGTTCTTTGCGGTTGTCGGTGCTAGTGGGAGTGTGTGGTATGTCATCAACAATGCACCAAGTATTTTCATGTTTGCTCTAGTTCAAGTTACAGTCCATCTTCTTGTAATTCTTGGTTTTGGAAAGCTATTCAGCATTGATTTAAAGCTGTTGCTTCTGGCTTCAAATGCCAACATTGGAGGCCCAACAACAGCATGTGGAATGGCAACTGCCAAGGGTTGGAGATCTTTGGTGGTTCCTTCTATTCTTGCTGGCATTTTCGGTATTGCAATTGCTACTTTCCTGGGCATCAGTTTTGGGGTGATGATCCTCAGGCGTTTGTAG

Coding sequence (CDS)

ATGGCGTCCATGATGAAGAACCCAGCCATGTTCGCGCCTCATTTTCCATTGCTGCAACTGCCTTCATTTTGCCACCGTTCGCCTCTCCCTCTCCTCCGTGGCCCTCCCTCAGCTGCCGCTGCCGCTTCAGTATCGAATTCCTCATTTCGGAAAGGAAGCGGCGACAGAGAGCTCCTCTCACTTTCGTCTTCCTCGGGATACCGGAATTCGACTCGGTATGTGAAGGTTAGGTCACAGCTGAGGCATCCAATTATTGCGCCGGATGATTATTGGGGCACATGGACTGCCTTGTTTGCTATTGGCGCATTGGGGATCTGGTCTGAGAAGACCACAATAGGTAGAACAGTGAGTGCTGCTTTGGTTAGCACTTTGGTTGGCCTGGCAGCCAGTAACTTTGGCATAATCCCCTATGAAGCCGTGTCTTATTCAATTGTCATGGAAATTCTGCTTCCAGTATCTGTTGCCTTGCTACTATTTCGGGCAGACATGCGCCATATAATCCGATCAACTGGAACATTACTTGGAGTCTTCATGCTCGGATCAGTTGCGACTATTATTGGAACTGTGGTAGCATTTCTCATGGTGCCTATGAGATCACTGGGTCCTGACAACTGGAAAATAGCTTCTGCTCTCATGGGTAGTTATATTGGTGGATCTGTTAATTATGTTGCAATTTCAGAGGCTCTTGGTGTTTCTCCATCAGTTTTAGCGGCAGGAGTGGCTGCTGACAATGTCATCAGTGCTTTATATTTTGTAGCCCTCTTTGCATTGGCTTCTAGAACACCTCCTGAACCCCCAACATCAACCAACGATGCTTCAACTGATATGCACTCTGATAACGGCACGAAGCTCCCTGTGTTGCCGACTGCTACGGCTGTTTTTGCATCATTTGCAATATGCAAATTTGTCACGTGGATTACAAATATATGTAAAATTCAGGGTGCCAATCTTCCTGGGATAACTGCAGTTGTTGTCATTTTAGCTACCGTTTTCCCAAAACAGTTCAGTTATTTAGCACCCGCAGCTGATACCATTGCCCTGATTCTTATGCAGGTGTTCTTTGCGGTTGTCGGTGCTAGTGGGAGTGTGTGGTATGTCATCAACAATGCACCAAGTATTTTCATGTTTGCTCTAGTTCAAGTTACAGTCCATCTTCTTGTAATTCTTGGTTTTGGAAAGCTATTCAGCATTGATTTAAAGCTGTTGCTTCTGGCTTCAAATGCCAACATTGGAGGCCCAACAACAGCATGTGGAATGGCAACTGCCAAGGGTTGGAGATCTTTGGTGGTTCCTTCTATTCTTGCTGGCATTTTCGGTATTGCAATTGCTACTTTCCTGGGCATCAGTTTTGGGGTGATGATCCTCAGGCGTTTGTAG

Protein sequence

MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSLSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALVSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICKFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASGSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMATAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Homology
BLAST of Carg08401 vs. NCBI nr
Match: KAG7035644.1 (yjcL [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 853.6 bits (2204), Expect = 7.9e-244
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL
Sbjct: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. NCBI nr
Match: XP_022958037.1 (uncharacterized protein LOC111459388 [Cucurbita moschata])

HSP 1 Score: 844.7 bits (2181), Expect = 3.7e-241
Identity = 454/458 (99.13%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSSSGYRNSTRYVKVRSQLRH IIAPDDYWGTWTALFAIG LGIWSEKTTIGRTVSAAL
Sbjct: 61  LSSSSGYRNSTRYVKVRSQLRHSIIAPDDYWGTWTALFAIGGLGIWSEKTTIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTSTNDASTDM SDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMDSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITN+CKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNLCKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. NCBI nr
Match: XP_023534574.1 (uncharacterized protein LOC111796113 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 452/458 (98.69%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSA AAASVSNSSFRKGSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAPAAASVSNSSFRKGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSS+GYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKT IGRTVSAAL
Sbjct: 61  LSSSAGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTNIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGT+VAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTLVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTSTNDASTDM SDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMDSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITN+CKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNLCKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. NCBI nr
Match: XP_022995261.1 (uncharacterized protein LOC111490862 [Cucurbita maxima])

HSP 1 Score: 840.1 bits (2169), Expect = 9.0e-240
Identity = 452/458 (98.69%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHR PLPLLRGPPSA AAASVSNSSFR+GSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRLPLPLLRGPPSAPAAASVSNSSFRRGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL
Sbjct: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTS NDASTDM SDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSINDASTDMDSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITNICKIQGANLPGITAVVVILA VFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNICKIQGANLPGITAVVVILAPVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. NCBI nr
Match: KGN49926.1 (hypothetical protein Csa_000607 [Cucumis sativus])

HSP 1 Score: 737.3 bits (1902), Expect = 8.2e-209
Identity = 396/452 (87.61%), Postives = 415/452 (91.81%), Query Frame = 0

Query: 7   NPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSLSSSSG 66
           NPAMFAPHFPLLQLPSFCH S LPLL G  SA    SVS SSFR+ +GDR LLS SSSS 
Sbjct: 8   NPAMFAPHFPLLQLPSFCHHSLLPLLHGRLSAPVPVSVSKSSFRRANGDRHLLSSSSSSS 67

Query: 67  YRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALVSTLVG 126
           Y NSTR VKVRSQLRHPIIA DDYWGTWTALFAIG LGIWSEKT +G TVSAALVSTLVG
Sbjct: 68  YSNSTRNVKVRSQLRHPIIAADDYWGTWTALFAIGTLGIWSEKTKVGSTVSAALVSTLVG 127

Query: 127 LAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATII 186
           LAASNFGIIPYEA+ YSIVME LLP+SV LLLFRAD+RHIIRSTGTLLGVF+LGSVATII
Sbjct: 128 LAASNFGIIPYEAMPYSIVMEFLLPLSVPLLLFRADIRHIIRSTGTLLGVFLLGSVATII 187

Query: 187 GTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI 246
           GTVVAFLMVPMRSLGPDNWK+A+ALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI
Sbjct: 188 GTVVAFLMVPMRSLGPDNWKVAAALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI 247

Query: 247 SALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICKFVTWI 306
           SALYFVALFALASRTPPEP TST+DASTD   D+GTKLPVL TATAV  SFAICKFVTWI
Sbjct: 248 SALYFVALFALASRTPPEPLTSTDDASTDKDFDHGTKLPVLQTATAVVTSFAICKFVTWI 307

Query: 307 TNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASGSVWYV 366
           TN+CKIQGANLPGITAVVVILAT+ PKQF+YLAPAADTIALILMQVFFAVVGASGS+WYV
Sbjct: 308 TNMCKIQGANLPGITAVVVILATILPKQFNYLAPAADTIALILMQVFFAVVGASGSIWYV 367

Query: 367 INNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMATAKGWR 426
           INN PSIFMFALVQVTVHL +IL FGKLF IDLKLLLLASNANIGGPTTACGMATAKGWR
Sbjct: 368 INNTPSIFMFALVQVTVHLAIILCFGKLFRIDLKLLLLASNANIGGPTTACGMATAKGWR 427

Query: 427 SLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           SLVVPSILAGIFGIAIATFLG+ FG+MILR +
Sbjct: 428 SLVVPSILAGIFGIAIATFLGVGFGLMILRHI 459

BLAST of Carg08401 vs. ExPASy Swiss-Prot
Match: O31634 (Uncharacterized membrane protein YjcL OS=Bacillus subtilis (strain 168) OX=224308 GN=yjcL PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.1e-35
Identity = 117/392 (29.85%), Postives = 196/392 (50.00%), Query Frame = 0

Query: 82  HPIIAPDDYWGTW--TALFAIGALGIWSEKTTIGRTVSAALVSTLVGLAASNFGIIPYEA 141
           H +I+ DD W  W   A++A  ++G+  ++      VS A+++    +  +N G++P E+
Sbjct: 2   HSLISSDDVWVLWGFIAVWAAVSIGL-EQRFKWASAVSGAIIALAGAMVFTNVGVLPVES 61

Query: 142 VSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATIIGTVVAFLMVPMRS 201
             Y  V   ++P+++ LLLF+ ++R I + +  LL +F++ SV T++G+++AF ++    
Sbjct: 62  PVYDTVWSYVVPLAIPLLLFQINVRQIFKESRRLLFIFLISSVGTVLGSILAFFLLKQHI 121

Query: 202 LGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVISALYFVALFALAS 261
             P   KI   +  SYIGG VN+ A++         ++A V ADN + AL F  L ++ +
Sbjct: 122 --PYLDKIGGMISASYIGGGVNFAAMAAKFETPGEYVSATVVADNFMMALLFFILISIPA 181

Query: 262 --------RTPPEPP-----TSTNDASTDMHSDNGTKLPVLPTATAVFASFAICKFVTWI 321
                     P E        S N A +     + +   +   A A FA  A+   V+  
Sbjct: 182 LKWFQRHYAMPFEEKVKADGNSGNSAESYWKRKDISLKDIAFNAGAAFALVAVSMKVSGY 241

Query: 322 TN-------ICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGA 381
                    +    G     +T++ V++  +FP+ F  L   +  +   L+ +FF V+G 
Sbjct: 242 FKSIFSHPLLTGTLGDQYLVLTSLTVLIIFLFPRFFERL-NGSQELGTFLIYLFFVVIGI 301

Query: 382 SGSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGM 441
              +  ++ NAP I +F  +    +L V L  GKLF + L+ +LLA NA +GGPTTA  M
Sbjct: 302 PADLRLIVTNAPLILLFVFIIAISNLAVSLAAGKLFRVRLEEILLAVNATVGGPTTAAAM 361

Query: 442 ATAKGWRSLVVPSILAGIFGIAIATFLGISFG 452
           A AKGWR LV P +L G  G  I  ++G   G
Sbjct: 362 AIAKGWRELVAPIMLVGTLGYLIGNYVGTFMG 389

BLAST of Carg08401 vs. ExPASy TrEMBL
Match: A0A6J1H0T8 (uncharacterized protein LOC111459388 OS=Cucurbita moschata OX=3662 GN=LOC111459388 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.8e-241
Identity = 454/458 (99.13%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSSSGYRNSTRYVKVRSQLRH IIAPDDYWGTWTALFAIG LGIWSEKTTIGRTVSAAL
Sbjct: 61  LSSSSGYRNSTRYVKVRSQLRHSIIAPDDYWGTWTALFAIGGLGIWSEKTTIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTSTNDASTDM SDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMDSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITN+CKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNLCKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. ExPASy TrEMBL
Match: A0A6J1K1H2 (uncharacterized protein LOC111490862 OS=Cucurbita maxima OX=3661 GN=LOC111490862 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.4e-240
Identity = 452/458 (98.69%), Postives = 453/458 (98.91%), Query Frame = 0

Query: 1   MASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLS 60
           MASMMKNPAMFAPHFPLLQLPSFCHR PLPLLRGPPSA AAASVSNSSFR+GSGDRELLS
Sbjct: 1   MASMMKNPAMFAPHFPLLQLPSFCHRLPLPLLRGPPSAPAAASVSNSSFRRGSGDRELLS 60

Query: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120
           LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL
Sbjct: 61  LSSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAAL 120

Query: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180
           VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG
Sbjct: 121 VSTLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLG 180

Query: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240
           SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV
Sbjct: 181 SVATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGV 240

Query: 241 AADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAIC 300
           AADNVISALYFVALFALASRTPPEPPTS NDASTDM SDNGTKLPVLPTATAVFASFAIC
Sbjct: 241 AADNVISALYFVALFALASRTPPEPPTSINDASTDMDSDNGTKLPVLPTATAVFASFAIC 300

Query: 301 KFVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360
           KFVTWITNICKIQGANLPGITAVVVILA VFPKQFSYLAPAADTIALILMQVFFAVVGAS
Sbjct: 301 KFVTWITNICKIQGANLPGITAVVVILAPVFPKQFSYLAPAADTIALILMQVFFAVVGAS 360

Query: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420
           GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA
Sbjct: 361 GSVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMA 420

Query: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL
Sbjct: 421 TAKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 458

BLAST of Carg08401 vs. ExPASy TrEMBL
Match: A0A0A0KQE5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140510 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.0e-209
Identity = 396/452 (87.61%), Postives = 415/452 (91.81%), Query Frame = 0

Query: 7   NPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSLSSSSG 66
           NPAMFAPHFPLLQLPSFCH S LPLL G  SA    SVS SSFR+ +GDR LLS SSSS 
Sbjct: 8   NPAMFAPHFPLLQLPSFCHHSLLPLLHGRLSAPVPVSVSKSSFRRANGDRHLLSSSSSSS 67

Query: 67  YRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALVSTLVG 126
           Y NSTR VKVRSQLRHPIIA DDYWGTWTALFAIG LGIWSEKT +G TVSAALVSTLVG
Sbjct: 68  YSNSTRNVKVRSQLRHPIIAADDYWGTWTALFAIGTLGIWSEKTKVGSTVSAALVSTLVG 127

Query: 127 LAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATII 186
           LAASNFGIIPYEA+ YSIVME LLP+SV LLLFRAD+RHIIRSTGTLLGVF+LGSVATII
Sbjct: 128 LAASNFGIIPYEAMPYSIVMEFLLPLSVPLLLFRADIRHIIRSTGTLLGVFLLGSVATII 187

Query: 187 GTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI 246
           GTVVAFLMVPMRSLGPDNWK+A+ALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI
Sbjct: 188 GTVVAFLMVPMRSLGPDNWKVAAALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVI 247

Query: 247 SALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICKFVTWI 306
           SALYFVALFALASRTPPEP TST+DASTD   D+GTKLPVL TATAV  SFAICKFVTWI
Sbjct: 248 SALYFVALFALASRTPPEPLTSTDDASTDKDFDHGTKLPVLQTATAVVTSFAICKFVTWI 307

Query: 307 TNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASGSVWYV 366
           TN+CKIQGANLPGITAVVVILAT+ PKQF+YLAPAADTIALILMQVFFAVVGASGS+WYV
Sbjct: 308 TNMCKIQGANLPGITAVVVILATILPKQFNYLAPAADTIALILMQVFFAVVGASGSIWYV 367

Query: 367 INNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMATAKGWR 426
           INN PSIFMFALVQVTVHL +IL FGKLF IDLKLLLLASNANIGGPTTACGMATAKGWR
Sbjct: 368 INNTPSIFMFALVQVTVHLAIILCFGKLFRIDLKLLLLASNANIGGPTTACGMATAKGWR 427

Query: 427 SLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           SLVVPSILAGIFGIAIATFLG+ FG+MILR +
Sbjct: 428 SLVVPSILAGIFGIAIATFLGVGFGLMILRHI 459

BLAST of Carg08401 vs. ExPASy TrEMBL
Match: A0A5A7TII9 (Putative membrane protein YjcL-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00980 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.8e-208
Identity = 395/457 (86.43%), Postives = 418/457 (91.47%), Query Frame = 0

Query: 2   ASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSL 61
           +S + NPAMFAPHFPLLQLPSFCH S LPLL G  SA    SV  SSFR+ +GDR LLS 
Sbjct: 8   SSRITNPAMFAPHFPLLQLPSFCHHSLLPLLHGRLSAPVPVSVPKSSFRRANGDRHLLSP 67

Query: 62  SSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALV 121
           SSSS YRNS++ VKVRSQLRHPIIA DDYWGTWTALFAIG LGIWSEKT IG TVSAALV
Sbjct: 68  SSSSSYRNSSQNVKVRSQLRHPIIAADDYWGTWTALFAIGTLGIWSEKTKIGSTVSAALV 127

Query: 122 STLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGS 181
           STLVGLAASNFGIIPYEA+ YSIVME LLP+SV LLLFRAD+RHI+R+TGTLLGVF+LGS
Sbjct: 128 STLVGLAASNFGIIPYEAMPYSIVMEFLLPLSVPLLLFRADIRHILRTTGTLLGVFLLGS 187

Query: 182 VATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVA 241
           VATIIGTVVAFLMVPMRSLGPDNWKIA+ALMGSYIGGSVNYVAISEALGVSPSVLAAGVA
Sbjct: 188 VATIIGTVVAFLMVPMRSLGPDNWKIAAALMGSYIGGSVNYVAISEALGVSPSVLAAGVA 247

Query: 242 ADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICK 301
           ADNVI+ALYFVALFALASRTPPEP TST+DASTD   D+GTKLPVL TATAV  SFAICK
Sbjct: 248 ADNVITALYFVALFALASRTPPEPLTSTDDASTDKDFDHGTKLPVLQTATAVVTSFAICK 307

Query: 302 FVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASG 361
           FVTWITN+CKIQGANLPGITAVVVILAT+ PKQF+YLAPAADTIALILMQVFFAVVGASG
Sbjct: 308 FVTWITNMCKIQGANLPGITAVVVILATILPKQFNYLAPAADTIALILMQVFFAVVGASG 367

Query: 362 SVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMAT 421
           SVWYVINN PSIFMFALVQVTVHL +IL FGKLF IDLKLLLLASNANIGGPTTACGMAT
Sbjct: 368 SVWYVINNTPSIFMFALVQVTVHLAIILCFGKLFRIDLKLLLLASNANIGGPTTACGMAT 427

Query: 422 AKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           AKGWRSLVVPSILAGIFGIAIATFLG+ FG+MILR +
Sbjct: 428 AKGWRSLVVPSILAGIFGIAIATFLGVGFGLMILRHI 464

BLAST of Carg08401 vs. ExPASy TrEMBL
Match: A0A1S3AU88 (uncharacterized membrane protein YjcL-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482913 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.8e-208
Identity = 395/457 (86.43%), Postives = 418/457 (91.47%), Query Frame = 0

Query: 2   ASMMKNPAMFAPHFPLLQLPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSL 61
           +S + NPAMFAPHFPLLQLPSFCH S LPLL G  SA    SV  SSFR+ +GDR LLS 
Sbjct: 8   SSRITNPAMFAPHFPLLQLPSFCHHSLLPLLHGRLSAPVPVSVPKSSFRRANGDRHLLSP 67

Query: 62  SSSSGYRNSTRYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALV 121
           SSSS YRNS++ VKVRSQLRHPIIA DDYWGTWTALFAIG LGIWSEKT IG TVSAALV
Sbjct: 68  SSSSSYRNSSQNVKVRSQLRHPIIAADDYWGTWTALFAIGTLGIWSEKTKIGSTVSAALV 127

Query: 122 STLVGLAASNFGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGS 181
           STLVGLAASNFGIIPYEA+ YSIVME LLP+SV LLLFRAD+RHI+R+TGTLLGVF+LGS
Sbjct: 128 STLVGLAASNFGIIPYEAMPYSIVMEFLLPLSVPLLLFRADIRHILRTTGTLLGVFLLGS 187

Query: 182 VATIIGTVVAFLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVA 241
           VATIIGTVVAFLMVPMRSLGPDNWKIA+ALMGSYIGGSVNYVAISEALGVSPSVLAAGVA
Sbjct: 188 VATIIGTVVAFLMVPMRSLGPDNWKIAAALMGSYIGGSVNYVAISEALGVSPSVLAAGVA 247

Query: 242 ADNVISALYFVALFALASRTPPEPPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICK 301
           ADNVI+ALYFVALFALASRTPPEP TST+DASTD   D+GTKLPVL TATAV  SFAICK
Sbjct: 248 ADNVITALYFVALFALASRTPPEPLTSTDDASTDKDFDHGTKLPVLQTATAVVTSFAICK 307

Query: 302 FVTWITNICKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASG 361
           FVTWITN+CKIQGANLPGITAVVVILAT+ PKQF+YLAPAADTIALILMQVFFAVVGASG
Sbjct: 308 FVTWITNMCKIQGANLPGITAVVVILATILPKQFNYLAPAADTIALILMQVFFAVVGASG 367

Query: 362 SVWYVINNAPSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMAT 421
           SVWYVINN PSIFMFALVQVTVHL +IL FGKLF IDLKLLLLASNANIGGPTTACGMAT
Sbjct: 368 SVWYVINNTPSIFMFALVQVTVHLAIILCFGKLFRIDLKLLLLASNANIGGPTTACGMAT 427

Query: 422 AKGWRSLVVPSILAGIFGIAIATFLGISFGVMILRRL 459
           AKGWRSLVVPSILAGIFGIAIATFLG+ FG+MILR +
Sbjct: 428 AKGWRSLVVPSILAGIFGIAIATFLGVGFGLMILRHI 464

BLAST of Carg08401 vs. TAIR 10
Match: AT5G24000.1 (Protein of unknown function (DUF819) )

HSP 1 Score: 472.2 bits (1214), Expect = 4.6e-133
Identity = 256/389 (65.81%), Postives = 307/389 (78.92%), Query Frame = 0

Query: 72  RYVKVRSQLRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALVSTLVGLAASN 131
           R VKV SQLR P+I+PDD+W  W ALFA GA G+WSEKT IG  VS AL STL+GLAASN
Sbjct: 56  RRVKVYSQLRFPLISPDDHWSQWAALFAAGAFGVWSEKTKIGSMVSGALTSTLLGLAASN 115

Query: 132 FGIIPYEAVSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATIIGTVVA 191
             +IP+E  SY   ME LLP ++ LLLFRAD+R IIRSTG+LL  F++GSVATI+GTVVA
Sbjct: 116 LRLIPFETPSYGFFMEFLLPHTIPLLLFRADLRRIIRSTGSLLLAFLIGSVATIVGTVVA 175

Query: 192 FLMVPMRSLGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVISALYF 251
           F++VPMRSLGPDNWKIA+ALMGSYIGGS+N+VAISEAL +SPSV+AAGVA DNVI AL+F
Sbjct: 176 FMLVPMRSLGPDNWKIAAALMGSYIGGSLNFVAISEALQISPSVIAAGVAVDNVICALHF 235

Query: 252 VALFALASRTPPEPPTSTNDASTDMHSDN--GTKLPVLPTATAVFASFAICKFVTWITNI 311
           + LFALAS+ PPE   S +    DM  D+    K  V+ T+ A+  SF ICK    +T +
Sbjct: 236 MVLFALASKIPPE-TASASSPDADMTKDDKLEDKNRVVSTSIALSVSFLICKAAITLTTL 295

Query: 312 CKIQGANLPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASGSVWYVINN 371
            KIQG  LP +TA+ ++LAT FP  F+ LAP+A+TI+LILMQVFF ++GA+GSVW VIN 
Sbjct: 296 FKIQGVMLPAVTAITIVLATSFPDFFNSLAPSAETISLILMQVFFTILGATGSVWNVINT 355

Query: 372 APSIFMFALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMATAKGWRSLV 431
           APSIF+FA +QV VHL V L  GKLF ID+KLLLLASNANIGGPTTAC MATAKGW SLV
Sbjct: 356 APSIFLFAAIQVMVHLAVTLVLGKLFCIDMKLLLLASNANIGGPTTACAMATAKGWTSLV 415

Query: 432 VPSILAGIFGIAIATFLGISFGVMILRRL 459
           VP IL+G+FG++IATFLGI  GV +L+RL
Sbjct: 416 VPGILSGVFGVSIATFLGIGCGVFVLKRL 443

BLAST of Carg08401 vs. TAIR 10
Match: AT5G52540.1 (Protein of unknown function (DUF819) )

HSP 1 Score: 469.2 bits (1206), Expect = 3.9e-132
Identity = 270/440 (61.36%), Postives = 330/440 (75.00%), Query Frame = 0

Query: 20  LPSFCHRSPLPLLRGPPSAAAAASVSNSSFRKGSGDRELLSLSSSSGYRNSTRYVKVRSQ 79
           LP F  +S    L   P+       S S FR G+ DR  LS  SSS  R+ T      S 
Sbjct: 34  LPEFSAKSTSSRLSFSPA-------SLSQFRNGT-DR-FLSPESSSPIRSVT-----VSS 93

Query: 80  LRHPIIAPDDYWGTWTALFAIGALGIWSEKTTIGRTVSAALVSTLVGLAASNFGIIPYEA 139
           L  P+I+P+D WGTWTALFA GALG+WSEKT +G  +S ALVSTLVGLAASN GII  +A
Sbjct: 94  LSTPLISPNDEWGTWTALFATGALGLWSEKTKVGAAMSGALVSTLVGLAASNLGIISSQA 153

Query: 140 VSYSIVMEILLPVSVALLLFRADMRHIIRSTGTLLGVFMLGSVATIIGTVVAFLMVPMRS 199
            ++++V+  LLP++V LLLFRAD+R +++STG LL  F++GSVAT +GT +A+ +VPM+S
Sbjct: 154 PAFAVVLNFLLPLAVPLLLFRADLRRVVQSTGKLLLAFLIGSVATTVGTALAYYLVPMKS 213

Query: 200 LGPDNWKIASALMGSYIGGSVNYVAISEALGVSPSVLAAGVAADNVISALYFVALFALAS 259
           LGPD+WKIA+ALMG +IGG+VNYVAIS ALGV+PSVLAAG+AADNVI A+YF  LFAL S
Sbjct: 214 LGPDSWKIAAALMGRHIGGAVNYVAISNALGVTPSVLAAGLAADNVICAVYFTTLFALGS 273

Query: 260 RTPPE---PPTSTNDASTDMHSDNGTKLPVLPTATAVFASFAICKFVTWITNICKIQGAN 319
           + P E   PPT+  DA T+  S+   K+PVL  AT +  S AICK    +T    I G +
Sbjct: 274 KIPAEAVPPPTTIVDAETNEASETKNKIPVLLIATGIAVSLAICKAGALLTKYFGISGGS 333

Query: 320 LPGITAVVVILATVFPKQFSYLAPAADTIALILMQVFFAVVGASGSVWYVINNAPSIFMF 379
           LP ITAVVVILATVFP QF  LAP+ + +ALILMQVFF VVGASG++W VIN APSIF+F
Sbjct: 334 LPAITAVVVILATVFPSQFGRLAPSGEAMALILMQVFFTVVGASGNIWSVINTAPSIFLF 393

Query: 380 ALVQVTVHLLVILGFGKLFSIDLKLLLLASNANIGGPTTACGMATAKGWRSLVVPSILAG 439
           ALVQ+  HL VILG GKL +I+L+LLLLASNAN+GGPTTA GMATAKGW SL+VP ILAG
Sbjct: 394 ALVQIGTHLAVILGIGKLLNIELRLLLLASNANVGGPTTAAGMATAKGWNSLIVPGILAG 453

Query: 440 IFGIAIATFLGISFGVMILR 457
           IFGIAIATF+GI+FGV +L+
Sbjct: 454 IFGIAIATFIGIAFGVKVLK 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035644.17.9e-244100.00yjcL [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958037.13.7e-24199.13uncharacterized protein LOC111459388 [Cucurbita moschata][more]
XP_023534574.11.1e-24098.69uncharacterized protein LOC111796113 [Cucurbita pepo subsp. pepo][more]
XP_022995261.19.0e-24098.69uncharacterized protein LOC111490862 [Cucurbita maxima][more]
KGN49926.18.2e-20987.61hypothetical protein Csa_000607 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
O316341.1e-3529.85Uncharacterized membrane protein YjcL OS=Bacillus subtilis (strain 168) OX=22430... [more]
Match NameE-valueIdentityDescription
A0A6J1H0T81.8e-24199.13uncharacterized protein LOC111459388 OS=Cucurbita moschata OX=3662 GN=LOC1114593... [more]
A0A6J1K1H24.4e-24098.69uncharacterized protein LOC111490862 OS=Cucurbita maxima OX=3661 GN=LOC111490862... [more]
A0A0A0KQE54.0e-20987.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140510 PE=4 SV=1[more]
A0A5A7TII95.8e-20886.43Putative membrane protein YjcL-like isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3AU885.8e-20886.43uncharacterized membrane protein YjcL-like isoform X1 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G24000.14.6e-13365.81Protein of unknown function (DUF819) [more]
AT5G52540.13.9e-13261.36Protein of unknown function (DUF819) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008537Protein of unknown function DUF819PFAMPF05684DUF819coord: 95..455
e-value: 3.7E-113
score: 378.4
IPR008537Protein of unknown function DUF819PANTHERPTHR34289PROTEIN, PUTATIVE (DUF819)-RELATEDcoord: 59..458
NoneNo IPR availablePANTHERPTHR34289:SF5KERATIN-ASSOCIATED PROTEIN (DUF819)coord: 59..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08401-RACarg08401-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity