Carg08396 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08396
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWRKY transcription factor
LocationCarg_Chr02: 5964206 .. 5965494 (+)
RNA-Seq ExpressionCarg08396
SyntenyCarg08396
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGGAGACGACGACTGGGATCTCTCCGCCGTCGTCCGGAGCTGCTATTCAGGCTCCTCCACGTCCTCCTCCTCCACCGCCGCCGACCCAACTGCCGCTTCCGCCGCAGAAACCGCCTTGTCTTGTCTCGCTTCCTTAACTTTTGACGACGATCCAAACAACGTCGCATTCTCCTTTTCCGACATCCTTCACCCCCAACAGCCCAACGGCTTCCATGAATTACACCAAGCCTTCATTTCTTTCCTCCCCAACCCCGTCGCCCCCGCCGTCGCCCCCGCCGCACCGGAAATCCCCAATCCGGTTCCAACCCGCCAATCCCGACACGGAATTAAACCCATTCGACCACAGCCAGATCCCGGAGCGGTGGCGCTGCAATCCCATCACCGGCAACAGCCACTTTTCCGGCCAGACTTCCCAAATTCACCAATGACGCCTCCTCACATACCCAAATCGAGAAAAAGGCAAGTAATTTAATAAAATTAAATTTACATTGAATTAAATCTTAATTGATTGATTTCTTTGTTGTCATAATAAAATTATTTTAAAATTGAAATTTATTTATTTATTTATTTATTTTTCTTTTAATTATTGTTATAAATTATTATTTATTTACAGAAAGAATCAACAGAAGAGAACCGTATGCCATGTGACTGCTGATAATCTCTCAACAGACATGTGGGCTTGGCGTAAATATGGGCAAAAGCCCATTAAAGGATCTCCCTACCCAAGGTATTTAAATATATAATATATATATATATATATATATATATAATTAATTAAATCAGGTCACGACTCTTTGTAACGATATGAAATTTGTTTGTTTTTCGGAAAAATTAACTTCCTCCTCCGATGATCATAGGAACTATTACCGTTGCAGCAGCTCAAAAGGGTGTGGGGCAAGAAAGCAAGTTGAACGGAGCAACGCCGACCCAGAAACTTTCATCGTCACATACACCGGAGATCACACTCATCCACGTCCAACTCATCGGAATTCCCTTGCCGGGAGTTCCCGAAATAGGTCGTCGTCTGCAATTCCCACGAAAAACCTGACAATAGGAGACTCCGATCGGTCATTGCTCGGCACAGCCACCGTGGGCTCTCCGATGACACCGCTTAACGAAGACGGTGGGAGCGGAGAGAAAGAAGGAGAGATTTTGGAGGATTTGAGCATTGATAGTGAGGAGTACGAGGATAATGATATTCTGATTCCAAATTTGATGATGAGAAATGAAATCTTTGTTGGGTTTGAGGAGCTTGGTTGTAGTCCTCGAAGGAAGGCTTGA

mRNA sequence

ATGGCTGCCGGAGACGACGACTGGGATCTCTCCGCCGTCGTCCGGAGCTGCTATTCAGGCTCCTCCACGTCCTCCTCCTCCACCGCCGCCGACCCAACTGCCGCTTCCGCCGCAGAAACCGCCTTGTCTTGTCTCGCTTCCTTAACTTTTGACGACGATCCAAACAACGTCGCATTCTCCTTTTCCGACATCCTTCACCCCCAACAGCCCAACGGCTTCCATGAATTACACCAAGCCTTCATTTCTTTCCTCCCCAACCCCGTCGCCCCCGCCGTCGCCCCCGCCGCACCGGAAATCCCCAATCCGGTTCCAACCCGCCAATCCCGACACGGAATTAAACCCATTCGACCACAGCCAGATCCCGGAGCGGTGGCGCTGCAATCCCATCACCGGCAACAGCCACTTTTCCGGCCAGACTTCCCAAATTCACCAATGACGCCTCCTCACATACCCAAATCGAGAAAAAGGCAAAATCAACAGAAGAGAACCGTATGCCATGTGACTGCTGATAATCTCTCAACAGACATGTGGGCTTGGCGTAAATATGGGCAAAAGCCCATTAAAGGATCTCCCTACCCAAGGAACTATTACCGTTGCAGCAGCTCAAAAGGGTGTGGGGCAAGAAAGCAAGTTGAACGGAGCAACGCCGACCCAGAAACTTTCATCGTCACATACACCGGAGATCACACTCATCCACGTCCAACTCATCGGAATTCCCTTGCCGGGAGTTCCCGAAATAGGTCGTCGTCTGCAATTCCCACGAAAAACCTGACAATAGGAGACTCCGATCGGTCATTGCTCGGCACAGCCACCGTGGGCTCTCCGATGACACCGCTTAACGAAGACGGTGGGAGCGGAGAGAAAGAAGGAGAGATTTTGGAGGATTTGAGCATTGATAGTGAGGAGTACGAGGATAATGATATTCTGATTCCAAATTTGATGATGAGAAATGAAATCTTTGTTGGGTTTGAGGAGCTTGGTTGTAGTCCTCGAAGGAAGGCTTGA

Coding sequence (CDS)

ATGGCTGCCGGAGACGACGACTGGGATCTCTCCGCCGTCGTCCGGAGCTGCTATTCAGGCTCCTCCACGTCCTCCTCCTCCACCGCCGCCGACCCAACTGCCGCTTCCGCCGCAGAAACCGCCTTGTCTTGTCTCGCTTCCTTAACTTTTGACGACGATCCAAACAACGTCGCATTCTCCTTTTCCGACATCCTTCACCCCCAACAGCCCAACGGCTTCCATGAATTACACCAAGCCTTCATTTCTTTCCTCCCCAACCCCGTCGCCCCCGCCGTCGCCCCCGCCGCACCGGAAATCCCCAATCCGGTTCCAACCCGCCAATCCCGACACGGAATTAAACCCATTCGACCACAGCCAGATCCCGGAGCGGTGGCGCTGCAATCCCATCACCGGCAACAGCCACTTTTCCGGCCAGACTTCCCAAATTCACCAATGACGCCTCCTCACATACCCAAATCGAGAAAAAGGCAAAATCAACAGAAGAGAACCGTATGCCATGTGACTGCTGATAATCTCTCAACAGACATGTGGGCTTGGCGTAAATATGGGCAAAAGCCCATTAAAGGATCTCCCTACCCAAGGAACTATTACCGTTGCAGCAGCTCAAAAGGGTGTGGGGCAAGAAAGCAAGTTGAACGGAGCAACGCCGACCCAGAAACTTTCATCGTCACATACACCGGAGATCACACTCATCCACGTCCAACTCATCGGAATTCCCTTGCCGGGAGTTCCCGAAATAGGTCGTCGTCTGCAATTCCCACGAAAAACCTGACAATAGGAGACTCCGATCGGTCATTGCTCGGCACAGCCACCGTGGGCTCTCCGATGACACCGCTTAACGAAGACGGTGGGAGCGGAGAGAAAGAAGGAGAGATTTTGGAGGATTTGAGCATTGATAGTGAGGAGTACGAGGATAATGATATTCTGATTCCAAATTTGATGATGAGAAATGAAATCTTTGTTGGGTTTGAGGAGCTTGGTTGTAGTCCTCGAAGGAAGGCTTGA

Protein sequence

MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFSFSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPDPGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDSEEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Homology
BLAST of Carg08396 vs. NCBI nr
Match: KAG7035649.1 (putative WRKY transcription factor 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667.2 bits (1720), Expect = 7.6e-188
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS
Sbjct: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. NCBI nr
Match: XP_022958605.1 (probable WRKY transcription factor 27 [Cucurbita moschata])

HSP 1 Score: 660.2 bits (1702), Expect = 9.3e-186
Identity = 330/334 (98.80%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSS+STAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSTSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKR+NQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRKNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIPTKNLTIGD DR LLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS
Sbjct: 241 AGSSRNRSSSAIPTKNLTIGDPDRLLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. NCBI nr
Match: XP_022996002.1 (probable WRKY transcription factor 27 [Cucurbita maxima])

HSP 1 Score: 656.8 bits (1693), Expect = 1.0e-184
Identity = 327/334 (97.90%), Postives = 331/334 (99.10%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAP APEIPNPVPTRQSRHGIKPIRPQPD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPVAPEIPNPVPTRQSRHGIKPIRPQPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKR+NQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRKNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERS ADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSTADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIPTKNLT+GD DRSL+GTATVGSPMTPLN+DGGSGEKEGEILEDLSIDS
Sbjct: 241 AGSSRNRSSSAIPTKNLTMGDPDRSLIGTATVGSPMTPLNDDGGSGEKEGEILEDLSIDS 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. NCBI nr
Match: XP_023534342.1 (probable WRKY transcription factor 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 325/334 (97.31%), Postives = 329/334 (98.50%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSS+STAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSTSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAP IPNPVPTRQSRHGIKPIRP PD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPVIPNPVPTRQSRHGIKPIRPLPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKR+NQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRKNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERS ADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSTADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIP KNLT+GD DRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSI+S
Sbjct: 241 AGSSRNRSSSAIPMKNLTMGDPDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIES 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. NCBI nr
Match: XP_038876070.1 (probable WRKY transcription factor 27 [Benincasa hispida])

HSP 1 Score: 458.8 bits (1179), Expect = 4.1e-125
Identity = 247/340 (72.65%), Postives = 279/340 (82.06%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAG+DDWDLSAVVRSC S  S +  ++AA   AA+AAE+ALSCLASLTF+DDPN+VAFS
Sbjct: 1   MAAGNDDWDLSAVVRSCNSAVSATDPTSAA---AAAAAESALSCLASLTFEDDPNDVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAA-----PEIPNPVPTRQSRHGIKPI 120
           FSDIL P+QPNGFHELHQAFISFLPNP A   A +A     PEIPN  P    RHGIKPI
Sbjct: 61  FSDILQPKQPNGFHELHQAFISFLPNPSAGPAAVSAPPAVVPEIPNSTPNHHFRHGIKPI 120

Query: 121 RPQPDPGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTD 180
           RP P P A+    HHRQ P F PDFP+SPMT   +PKSRKRQNQQKRTVCHVTADNLSTD
Sbjct: 121 RPNPHPAALQHHHHHRQPP-FPPDFPSSPMTQSLMPKSRKRQNQQKRTVCHVTADNLSTD 180

Query: 181 MWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPT 240
           MWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFI+TYTGDH+HPRPT
Sbjct: 181 MWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIITYTGDHSHPRPT 240

Query: 241 HRNSLAGSSRNRSSSA-IPTKNLTIGDSDRSLL-----GTATVGSPMTPLNED-GGSGEK 300
           HRNSLAGSSRNRSSSA IPT++ T GD D SL       ++   SP+TPLN+D GG GEK
Sbjct: 241 HRNSLAGSSRNRSSSASIPTRHPTTGDPDSSLTALVASSSSPAASPITPLNDDEGGGGEK 300

Query: 301 EGEILEDLSIDS-EEYEDNDILIPNLMMRNEIFVGFEELG 328
           + E+ ED++IDS ++ +D+DILIPNL +R+EIFVGF+ELG
Sbjct: 301 DEEMFEDMNIDSDDDDDDDDILIPNLTVRDEIFVGFDELG 336

BLAST of Carg08396 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-48
Identity = 143/347 (41.21%), Postives = 178/347 (51.30%), Query Frame = 0

Query: 6   DDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFSFSDIL 65
           +DWDL AVVRSC S  ST++S    +    +  +            D P    F      
Sbjct: 4   EDWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQ----------QDPPPPPLF------ 63

Query: 66  HPQQPNGFHELHQAFISFLPNPVA--------PAVAPAAPEIPNPVPTRQSRHGIKPIRP 125
             Q  +  +EL  +   FLP            P + P     P+P    +    +   + 
Sbjct: 64  --QASSSCNELQDSCKPFLPVTTTTTTTWSPPPLLPPPKASSPSPNILLKQEQVLLESQD 123

Query: 126 QPDPGAVALQSHHRQQPLF-----RPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNL 185
           Q  P +V +        +F     R         PP   +SRKR+NQQKRT+CHVT +NL
Sbjct: 124 QKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPP--LRSRKRKNQQKRTICHVTQENL 183

Query: 186 STDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHP 245
           S+D+WAWRKYGQKPIKGSPYPRNYYRCSSSKGC ARKQVERSN DP  FIVTYTG+HTHP
Sbjct: 184 SSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHP 243

Query: 246 RPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVG----SPMTPL------NED 305
           RPTHRNSLAGS+RN+S    P     +   D S L          SP TPL       E 
Sbjct: 244 RPTHRNSLAGSTRNKSQPVNP-----VPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQET 303

Query: 306 GGSGE--------KEGEILEDLSIDSEEYED----NDILIPNLMMRN 318
            G  +        +E E  E++  D EE ED    +D+LIPNL +R+
Sbjct: 304 NGDEDMVGQEVNMEEEEEEEEVEEDDEEEEDDDDVDDLLIPNLAVRD 325

BLAST of Carg08396 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.0e-35
Identity = 112/320 (35.00%), Postives = 162/320 (50.62%), Query Frame = 0

Query: 6   DDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFSFSDIL 65
           DDWDL AVVR C S  S+S+++T   P  +S            T     N V+F      
Sbjct: 3   DDWDLHAVVRGC-SAVSSSATTTVYSPGVSSHTNPI------FTVGRQSNAVSFG----- 62

Query: 66  HPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPDPGAVA 125
                    E+   +  F    V  + +     I  P   R+ ++  +P+      G+V 
Sbjct: 63  ---------EIRDLYTPFTQESVVSSFSC----INYPEEPRKPQNQKRPLSLSASSGSV- 122

Query: 126 LQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWRKYGQK 185
                            S  +  +  +S++R+ Q K+ VCHV A+ L++D+WAWRKYGQK
Sbjct: 123 ----------------TSKPSGSNTSRSKRRKIQHKK-VCHVAAEALNSDVWAWRKYGQK 182

Query: 186 PIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSR 245
           PIKGSPYPR YYRCS+SKGC ARKQVER+ +DP+ FIVTYT +H HP PTHRNSLAGS+R
Sbjct: 183 PIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTR 242

Query: 246 NRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDSEEYED 305
            + S    +K+ T   +  S     +    + P+ +    G+ +GE  EDL         
Sbjct: 243 QKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGE--EDL--------- 266

Query: 306 NDILIPNLMMRNEIFVGFEE 326
             + + + ++ ++ F G EE
Sbjct: 303 --LSLSDTVVSDDFFDGLEE 266

BLAST of Carg08396 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 76/144 (52.78%), Postives = 99/144 (68.75%), Query Frame = 0

Query: 152 KSRK-RQNQQKRTVCHVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQ 211
           KS+K ++NQQKR V  V  +NL +D WAWRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQ
Sbjct: 111 KSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQ 170

Query: 212 VERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTA 271
           VER+  +PE F +TYT +H H  PT RNSLAGS+R ++S   PT       S++ ++ + 
Sbjct: 171 VERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPT---LTKKSEKEVVSSP 230

Query: 272 TVGSPMTPLNEDGGSGEKEGEILE 295
           T  +PM P  ++     +E  + E
Sbjct: 231 T-SNPMIPSADESSVAVQEMSVAE 250

BLAST of Carg08396 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.0e-31
Identity = 62/108 (57.41%), Postives = 80/108 (74.07%), Query Frame = 0

Query: 154 RKRQNQQKRTVC---------HVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKG 213
           ++R++Q K+ VC           + + + +D+WAWRKYGQKPIKGSPYPR YYRCSSSKG
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 245

Query: 214 CGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSSSAI 253
           C ARKQVERS  DP   ++TYT +H HP PT RN+LAGS+R+ SSS++
Sbjct: 246 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSL 293

BLAST of Carg08396 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-30
Identity = 79/169 (46.75%), Postives = 104/169 (61.54%), Query Frame = 0

Query: 152  KSRKRQNQQKRTVCHVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQV 211
            KSR++ N++KR VC V   + S+D+W WRKYGQKPIK SPYPR+YYRC+SSKGC ARKQV
Sbjct: 1158 KSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQKPIKSSPYPRSYYRCASSKGCFARKQV 1217

Query: 212  ERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSS-------SAIPTKNLTIGDSDR 271
            ERS  DP   ++TY  +H HP PT RN+LAGS+R+ SS       SA  T +      D+
Sbjct: 1218 ERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVSQDKEGPDK 1277

Query: 272  SLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDSEEYEDNDILIPNL 314
            S L +    SP +P        E++ E  +++  D +  ED    IP L
Sbjct: 1278 SHLPS----SPASPPYAAMVVKEEDMEQWDNMEFDVDVEEDT--FIPEL 1320

BLAST of Carg08396 vs. ExPASy TrEMBL
Match: A0A6J1H3Z3 (probable WRKY transcription factor 27 OS=Cucurbita moschata OX=3662 GN=LOC111459781 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 4.5e-186
Identity = 330/334 (98.80%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSS+STAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSTSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKR+NQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRKNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIPTKNLTIGD DR LLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS
Sbjct: 241 AGSSRNRSSSAIPTKNLTIGDPDRLLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. ExPASy TrEMBL
Match: A0A6J1K0K0 (probable WRKY transcription factor 27 OS=Cucurbita maxima OX=3661 GN=LOC111491344 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.0e-185
Identity = 327/334 (97.90%), Postives = 331/334 (99.10%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS
Sbjct: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60

Query: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPD 120
           FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAP APEIPNPVPTRQSRHGIKPIRPQPD
Sbjct: 61  FSDILHPQQPNGFHELHQAFISFLPNPVAPAVAPVAPEIPNPVPTRQSRHGIKPIRPQPD 120

Query: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWR 180
           PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKR+NQQKRTVCHVTADNLSTDMWAWR
Sbjct: 121 PGAVALQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRKNQQKRTVCHVTADNLSTDMWAWR 180

Query: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSL 240
           KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERS ADPETFIVTYTGDHTHPRPTHRNSL
Sbjct: 181 KYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSTADPETFIVTYTGDHTHPRPTHRNSL 240

Query: 241 AGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDS 300
           AGSSRNRSSSAIPTKNLT+GD DRSL+GTATVGSPMTPLN+DGGSGEKEGEILEDLSIDS
Sbjct: 241 AGSSRNRSSSAIPTKNLTMGDPDRSLIGTATVGSPMTPLNDDGGSGEKEGEILEDLSIDS 300

Query: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 335
           EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA
Sbjct: 301 EEYEDNDILIPNLMMRNEIFVGFEELGCSPRRKA 334

BLAST of Carg08396 vs. ExPASy TrEMBL
Match: A0A5A7TGX7 (WRKY transcription factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00900 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.3e-118
Identity = 244/350 (69.71%), Postives = 277/350 (79.14%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAG+DDWDLSAVVRSC      +S+++A DPT+A AAE+ALSCLASLTFDDDP++VAFS
Sbjct: 1   MAAGNDDWDLSAVVRSC------NSAASATDPTSA-AAESALSCLASLTFDDDPDDVAFS 60

Query: 61  FSDILHPQQPN-GFHELHQAFISFLPNPVA-----------PAVAPAAPEIPNPV-PTRQ 120
           FSDI  P+QPN GFHELHQAF+SFLPNP A            AV  + PEIP P  P R 
Sbjct: 61  FSDIFQPKQPNGGFHELHQAFVSFLPNPSATDTTTTTTTTVTAVPVSEPEIPYPTPPNRH 120

Query: 121 SRHGIKPIRPQPDPGAVALQSH-HRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCH 180
            R GIKPIRP P P  VALQ H H +QP F PD PNSPMT   IPKSRKRQNQQKR VCH
Sbjct: 121 FRQGIKPIRPNPPP--VALQQHPHHRQPPFSPDLPNSPMTQSLIPKSRKRQNQQKRRVCH 180

Query: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYT 240
           VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSN DPETFI+TYT
Sbjct: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNVDPETFIITYT 240

Query: 241 GDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATV-------GSPMTPL 300
           GDH+HPRPTHRNSLAGSSRNRSSS+  +++ T GD D S+  +A V        SP+TPL
Sbjct: 241 GDHSHPRPTHRNSLAGSSRNRSSSS-SSRHPTPGDFDPSMTASALVPSSSSPAASPITPL 300

Query: 301 NE-DGGSGEKEGEILEDLSIDSEEYEDN-DILIPNLMMRNEIFVGFEELG 328
           N+ +G +GEK+GE+ ED+ IDS+E ED+ DILIPNL +R+EIFVGFEE+G
Sbjct: 301 NDYEGPTGEKDGEMFEDMPIDSDEEEDDEDILIPNLTVRDEIFVGFEEVG 340

BLAST of Carg08396 vs. ExPASy TrEMBL
Match: E5GBD4 (WRKY transcription factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.3e-118
Identity = 244/350 (69.71%), Postives = 277/350 (79.14%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAG+DDWDLSAVVRSC      +S+++A DPT+A AAE+ALSCLASLTFDDDP++VAFS
Sbjct: 1   MAAGNDDWDLSAVVRSC------NSAASATDPTSA-AAESALSCLASLTFDDDPDDVAFS 60

Query: 61  FSDILHPQQPN-GFHELHQAFISFLPNPVA-----------PAVAPAAPEIPNPV-PTRQ 120
           FSDI  P+QPN GFHELHQAF+SFLPNP A            AV  + PEIP P  P R 
Sbjct: 61  FSDIFQPKQPNGGFHELHQAFVSFLPNPSATDTTTTTTTTVTAVPVSEPEIPYPTPPNRH 120

Query: 121 SRHGIKPIRPQPDPGAVALQSH-HRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCH 180
            R GIKPIRP P P  VALQ H H +QP F PD PNSPMT   IPKSRKRQNQQKR VCH
Sbjct: 121 FRQGIKPIRPNPPP--VALQQHPHHRQPPFSPDLPNSPMTQSLIPKSRKRQNQQKRRVCH 180

Query: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYT 240
           VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSN DPETFI+TYT
Sbjct: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNVDPETFIITYT 240

Query: 241 GDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATV-------GSPMTPL 300
           GDH+HPRPTHRNSLAGSSRNRSSS+  +++ T GD D S+  +A V        SP+TPL
Sbjct: 241 GDHSHPRPTHRNSLAGSSRNRSSSS-SSRHPTPGDFDPSMTASALVPSSSSPAASPITPL 300

Query: 301 NE-DGGSGEKEGEILEDLSIDSEEYEDN-DILIPNLMMRNEIFVGFEELG 328
           N+ +G +GEK+GE+ ED+ IDS+E ED+ DILIPNL +R+EIFVGFEE+G
Sbjct: 301 NDYEGPTGEKDGEMFEDMPIDSDEEEDDEDILIPNLTVRDEIFVGFEEVG 340

BLAST of Carg08396 vs. ExPASy TrEMBL
Match: A0A1S3AU96 (probable WRKY transcription factor 27 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482922 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.5e-117
Identity = 243/350 (69.43%), Postives = 277/350 (79.14%), Query Frame = 0

Query: 1   MAAGDDDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFS 60
           MAAG+DDWDLSAVVRSC      +S+++A DPT+A AAE+ALSCLASLTFDDDP++VAFS
Sbjct: 1   MAAGNDDWDLSAVVRSC------NSAASATDPTSA-AAESALSCLASLTFDDDPDDVAFS 60

Query: 61  FSDILHPQQPN-GFHELHQAFISFLPNPVA-----------PAVAPAAPEIPNPV-PTRQ 120
           FSDI  P+QPN GFHELHQAF+SFLPNP A            AV  + PEIP P  P R 
Sbjct: 61  FSDIFQPKQPNGGFHELHQAFVSFLPNPSATDTTTTTTTTVTAVPVSEPEIPYPTPPNRH 120

Query: 121 SRHGIKPIRPQPDPGAVALQSH-HRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCH 180
            R GIKPIRP P P  VALQ H H +QP F PD PNSPMT   IPKSRKR+NQQKR VCH
Sbjct: 121 FRQGIKPIRPNPPP--VALQQHPHHRQPPFSPDLPNSPMTQSLIPKSRKRKNQQKRRVCH 180

Query: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYT 240
           VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSN DPETFI+TYT
Sbjct: 181 VTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNVDPETFIITYT 240

Query: 241 GDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATV-------GSPMTPL 300
           GDH+HPRPTHRNSLAGSSRNRSSS+  +++ T GD D S+  +A V        SP+TPL
Sbjct: 241 GDHSHPRPTHRNSLAGSSRNRSSSS-SSRHPTPGDFDPSMTASALVPSSSSPAASPITPL 300

Query: 301 NE-DGGSGEKEGEILEDLSIDSEEYEDN-DILIPNLMMRNEIFVGFEELG 328
           N+ +G +GEK+GE+ ED+ IDS+E ED+ DILIPNL +R+EIFVGFEE+G
Sbjct: 301 NDYEGPTGEKDGEMFEDMPIDSDEEEDDEDILIPNLTVRDEIFVGFEEVG 340

BLAST of Carg08396 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 193.0 bits (489), Expect = 3.9e-49
Identity = 143/347 (41.21%), Postives = 178/347 (51.30%), Query Frame = 0

Query: 6   DDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFSFSDIL 65
           +DWDL AVVRSC S  ST++S    +    +  +            D P    F      
Sbjct: 4   EDWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQ----------QDPPPPPLF------ 63

Query: 66  HPQQPNGFHELHQAFISFLPNPVA--------PAVAPAAPEIPNPVPTRQSRHGIKPIRP 125
             Q  +  +EL  +   FLP            P + P     P+P    +    +   + 
Sbjct: 64  --QASSSCNELQDSCKPFLPVTTTTTTTWSPPPLLPPPKASSPSPNILLKQEQVLLESQD 123

Query: 126 QPDPGAVALQSHHRQQPLF-----RPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNL 185
           Q  P +V +        +F     R         PP   +SRKR+NQQKRT+CHVT +NL
Sbjct: 124 QKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPP--LRSRKRKNQQKRTICHVTQENL 183

Query: 186 STDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHP 245
           S+D+WAWRKYGQKPIKGSPYPRNYYRCSSSKGC ARKQVERSN DP  FIVTYTG+HTHP
Sbjct: 184 SSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHP 243

Query: 246 RPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTATVG----SPMTPL------NED 305
           RPTHRNSLAGS+RN+S    P     +   D S L          SP TPL       E 
Sbjct: 244 RPTHRNSLAGSTRNKSQPVNP-----VPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQET 303

Query: 306 GGSGE--------KEGEILEDLSIDSEEYED----NDILIPNLMMRN 318
            G  +        +E E  E++  D EE ED    +D+LIPNL +R+
Sbjct: 304 NGDEDMVGQEVNMEEEEEEEEVEEDDEEEEDDDDVDDLLIPNLAVRD 325

BLAST of Carg08396 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 149.4 bits (376), Expect = 5.0e-36
Identity = 112/320 (35.00%), Postives = 162/320 (50.62%), Query Frame = 0

Query: 6   DDWDLSAVVRSCYSGSSTSSSSTAADPTAASAAETALSCLASLTFDDDPNNVAFSFSDIL 65
           DDWDL AVVR C S  S+S+++T   P  +S            T     N V+F      
Sbjct: 3   DDWDLHAVVRGC-SAVSSSATTTVYSPGVSSHTNPI------FTVGRQSNAVSFG----- 62

Query: 66  HPQQPNGFHELHQAFISFLPNPVAPAVAPAAPEIPNPVPTRQSRHGIKPIRPQPDPGAVA 125
                    E+   +  F    V  + +     I  P   R+ ++  +P+      G+V 
Sbjct: 63  ---------EIRDLYTPFTQESVVSSFSC----INYPEEPRKPQNQKRPLSLSASSGSV- 122

Query: 126 LQSHHRQQPLFRPDFPNSPMTPPHIPKSRKRQNQQKRTVCHVTADNLSTDMWAWRKYGQK 185
                            S  +  +  +S++R+ Q K+ VCHV A+ L++D+WAWRKYGQK
Sbjct: 123 ----------------TSKPSGSNTSRSKRRKIQHKK-VCHVAAEALNSDVWAWRKYGQK 182

Query: 186 PIKGSPYPRNYYRCSSSKGCGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSR 245
           PIKGSPYPR YYRCS+SKGC ARKQVER+ +DP+ FIVTYT +H HP PTHRNSLAGS+R
Sbjct: 183 PIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTR 242

Query: 246 NRSSSAIPTKNLTIGDSDRSLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDSEEYED 305
            + S    +K+ T   +  S     +    + P+ +    G+ +GE  EDL         
Sbjct: 243 QKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGE--EDL--------- 266

Query: 306 NDILIPNLMMRNEIFVGFEE 326
             + + + ++ ++ F G EE
Sbjct: 303 --LSLSDTVVSDDFFDGLEE 266

BLAST of Carg08396 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 142.1 bits (357), Expect = 8.0e-34
Identity = 76/144 (52.78%), Postives = 99/144 (68.75%), Query Frame = 0

Query: 152 KSRK-RQNQQKRTVCHVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQ 211
           KS+K ++NQQKR V  V  +NL +D WAWRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQ
Sbjct: 111 KSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQ 170

Query: 212 VERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSSSAIPTKNLTIGDSDRSLLGTA 271
           VER+  +PE F +TYT +H H  PT RNSLAGS+R ++S   PT       S++ ++ + 
Sbjct: 171 VERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPT---LTKKSEKEVVSSP 230

Query: 272 TVGSPMTPLNEDGGSGEKEGEILE 295
           T  +PM P  ++     +E  + E
Sbjct: 231 T-SNPMIPSADESSVAVQEMSVAE 250

BLAST of Carg08396 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 136.0 bits (341), Expect = 5.7e-32
Identity = 62/108 (57.41%), Postives = 80/108 (74.07%), Query Frame = 0

Query: 154 RKRQNQQKRTVC---------HVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKG 213
           ++R++Q K+ VC           + + + +D+WAWRKYGQKPIKGSPYPR YYRCSSSKG
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 245

Query: 214 CGARKQVERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSSSAI 253
           C ARKQVERS  DP   ++TYT +H HP PT RN+LAGS+R+ SSS++
Sbjct: 246 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSL 293

BLAST of Carg08396 vs. TAIR 10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 135.2 bits (339), Expect = 9.7e-32
Identity = 79/169 (46.75%), Postives = 104/169 (61.54%), Query Frame = 0

Query: 152  KSRKRQNQQKRTVCHVTADNLSTDMWAWRKYGQKPIKGSPYPRNYYRCSSSKGCGARKQV 211
            KSR++ N++KR VC V   + S+D+W WRKYGQKPIK SPYPR+YYRC+SSKGC ARKQV
Sbjct: 1158 KSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQKPIKSSPYPRSYYRCASSKGCFARKQV 1217

Query: 212  ERSNADPETFIVTYTGDHTHPRPTHRNSLAGSSRNRSS-------SAIPTKNLTIGDSDR 271
            ERS  DP   ++TY  +H HP PT RN+LAGS+R+ SS       SA  T +      D+
Sbjct: 1218 ERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVSQDKEGPDK 1277

Query: 272  SLLGTATVGSPMTPLNEDGGSGEKEGEILEDLSIDSEEYEDNDILIPNL 314
            S L +    SP +P        E++ E  +++  D +  ED    IP L
Sbjct: 1278 SHLPS----SPASPPYAAMVVKEEDMEQWDNMEFDVDVEEDT--FIPEL 1320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035649.17.6e-188100.00putative WRKY transcription factor 27, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022958605.19.3e-18698.80probable WRKY transcription factor 27 [Cucurbita moschata][more]
XP_022996002.11.0e-18497.90probable WRKY transcription factor 27 [Cucurbita maxima][more]
XP_023534342.11.3e-18297.31probable WRKY transcription factor 27 [Cucurbita pepo subsp. pepo][more]
XP_038876070.14.1e-12572.65probable WRKY transcription factor 27 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FLX85.5e-4841.21Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
O046097.0e-3535.00WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Q9SUS11.1e-3252.78Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ... [more]
O647478.0e-3157.41Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9FL921.4e-3046.75Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1H3Z34.5e-18698.80probable WRKY transcription factor 27 OS=Cucurbita moschata OX=3662 GN=LOC111459... [more]
A0A6J1K0K05.0e-18597.90probable WRKY transcription factor 27 OS=Cucurbita maxima OX=3661 GN=LOC11149134... [more]
A0A5A7TGX75.3e-11869.71WRKY transcription factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
E5GBD45.3e-11869.71WRKY transcription factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3AU961.5e-11769.43probable WRKY transcription factor 27 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G52830.13.9e-4941.21WRKY DNA-binding protein 27 [more]
AT4G01250.15.0e-3635.00WRKY family transcription factor [more]
AT4G23550.18.0e-3452.78WRKY family transcription factor [more]
AT2G34830.15.7e-3257.41WRKY DNA-binding protein 35 [more]
AT5G45050.19.7e-3246.75Disease resistance protein (TIR-NBS-LRR class) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 173..233
e-value: 3.8E-37
score: 139.4
IPR003657WRKY domainPFAMPF03106WRKYcoord: 175..232
e-value: 4.7E-26
score: 90.8
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 168..234
score: 32.856415
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 156..227
e-value: 1.9E-29
score: 103.1
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 168..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 236..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..162
NoneNo IPR availablePANTHERPTHR32096:SF80WRKY TRANSCRIPTION FACTOR 27-RELATEDcoord: 6..326
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 6..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08396-RACarg08396-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding