Carg08367 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08367 vs. NCBI nr
Match: KAG7029801.1 ((R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2890.9 bits (7493), Expect = 0.0e+00
Identity = 1433/1433 (100.00%), Postives = 1433/1433 (100.00%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60
            MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG
Sbjct: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60

Query: 61   LANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDM 120
            LANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDM
Sbjct: 61   LANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDM 120

Query: 121  TAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSR 180
            TAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSR
Sbjct: 121  TAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSR 180

Query: 181  GRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATISKNG 240
            GRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATISKNG
Sbjct: 181  GRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATISKNG 240

Query: 241  EIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLT 300
            EIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLT
Sbjct: 241  EIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLT 300

Query: 301  PPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVG 360
            PPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVG
Sbjct: 301  PPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVG 360

Query: 361  SLRLDGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLG 420
            SLRLDGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLG
Sbjct: 361  SLRLDGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLG 420

Query: 421  APLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLS 480
            APLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLS
Sbjct: 421  APLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLS 480

Query: 481  PGTNPMATVMMLGRYVGLKMLQQRLDVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLA 540
            PGTNPMATVMMLGRYVGLKMLQQRLDVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLA
Sbjct: 481  PGTNPMATVMMLGRYVGLKMLQQRLDVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLA 540

Query: 541  ATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRI 600
            ATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRI
Sbjct: 541  ATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRI 600

Query: 601  LGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSAL 660
            LGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSAL
Sbjct: 601  LGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSAL 660

Query: 661  LEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILF 720
            LEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILF
Sbjct: 661  LEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILF 720

Query: 721  NGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVV 780
            NGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVV
Sbjct: 721  NGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVV 780

Query: 781  HHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSL 840
            HHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSL
Sbjct: 781  HHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSL 840

Query: 841  LPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGV 900
            LPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGV
Sbjct: 841  LPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGV 900

Query: 901  RKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGG 960
            RKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGG
Sbjct: 901  RKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGG 960

Query: 961  CLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERSNISYM 1020
            CLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERSNISYM
Sbjct: 961  CLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERSNISYM 1020

Query: 1021 KFVHDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQAL 1080
            KFVHDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQAL
Sbjct: 1021 KFVHDGNDFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQAL 1080

Query: 1081 LNVFTVEDDGKNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFD 1140
            LNVFTVEDDGKNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFD
Sbjct: 1081 LNVFTVEDDGKNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFD 1140

Query: 1141 MEMVEKAYKWVEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDE 1200
            MEMVEKAYKWVEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDE
Sbjct: 1141 MEMVEKAYKWVEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDE 1200

Query: 1201 NGNRHGAIILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVP 1260
            NGNRHGAIILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVP
Sbjct: 1201 NGNRHGAIILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVP 1260

Query: 1261 ILPFPLDFTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTVATIGGKF 1320
            ILPFPLDFTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTVATIGGKF
Sbjct: 1261 ILPFPLDFTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTVATIGGKF 1320

Query: 1321 SKLTSVGSLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRD 1380
            SKLTSVGSLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRD
Sbjct: 1321 SKLTSVGSLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRD 1380

Query: 1381 LEGNRVIQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDVSSD 1434
            LEGNRVIQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDVSSD
Sbjct: 1381 LEGNRVIQFLGPPLPENLSDYSSVAEFCKRTVTTYWHYHGGCLVGKVVDVSSD 1433

BLAST of Carg08367 vs. NCBI nr
Match: KAF4383855.1 (hypothetical protein G4B88_016288 [Cannabis sativa])

HSP 1 Score: 1461.8 bits (3783), Expect = 0.0e+00
Identity = 775/1539 (50.36%), Postives = 1004/1539 (65.24%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60
            MK V N   +P  EEYDYI++GGGTAGCPLAATLS  +SVLVLERG+ P A P+ L   G
Sbjct: 35   MKSVYNATDMPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASG 94

Query: 61   LANTL--TDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQW 120
                L   D+DDG  P QRF SE+GVE++RGRVLGG SMIN  F+S A  +F   SG++W
Sbjct: 95   FLTNLVEADDDDGDTPAQRFTSEEGVESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEW 154

Query: 121  DMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFD 180
            D   VEKAY+W++  +VS   LS WQSA +EALLEAGVGPDNG   KH VGT+  GS FD
Sbjct: 155  DNDEVEKAYEWVKSKIVSYSNLSTWQSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFD 214

Query: 181  SRGRRHGAVELLNKADPRNLRVATQATVKRIIFS-QSNGLSASGVLYSDFKGKLHKATIS 240
            + GRRHGAVELLNK + +N+++A  A VK+IIFS +S+  SA GV+Y+D KGK HKA I 
Sbjct: 215  NMGRRHGAVELLNKGNLKNMKIAIHAYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIR 274

Query: 241  KNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLP 300
              GE+IL+AGA+GSP LLL SG+GPKS+LSS  +P+V    +VG+ MADNPR    +++P
Sbjct: 275  NKGEVILSAGALGSPQLLLLSGIGPKSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIP 334

Query: 301  FLTPPTSVQVVGTLKRNIHIESLSSILPFS-IFPPFGLLPPRSTAVNLSLAVFAGKFSTV 360
            F    +  QVVG +  +  IE++S  LPF+    PF   P   T    SLA    K    
Sbjct: 335  FPFEGSGAQVVG-ITDDYFIETVSYSLPFAPTTLPFSFYPNPLTPPQFSLASIVEKIVGP 394

Query: 361  SSVGSLRLDGRKN----PIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEG 420
             S GSL L   K+    P VRFNY S+P D+ RCV  +RKVG+++ TKS++R K  DL G
Sbjct: 395  LSTGSLTLASTKDVKITPHVRFNYFSNPIDLARCVSAMRKVGEMLETKSLDRFKYKDLNG 454

Query: 421  KKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVV 480
             + F +LG PLP N SD   + D+CR +VTTFWHYHGGCLVGKVVD +++VIG   LRVV
Sbjct: 455  ARDFMYLGPPLPANQSDNSAMEDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGTNSLRVV 514

Query: 481  DGSTFSLSPGTNPMATVMMLGRY--------VGLKMLQQRLDVSYMKFVHNAGDLPEKQE 540
            DGSTF +SPGTNP AT+MM+GR         +G+ +     D SYMK V+NA D+P ++E
Sbjct: 515  DGSTFVISPGTNPQATLMMIGRVGDNNGPFGLGIAVSGADYDFSYMKSVYNATDMPLEEE 574

Query: 541  YDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLL-NAFLAQDDGQNP 600
            YDYI++GGG AGCPLAATLS K+ VL+LERG+ P  +P+ L   G L N   A DDG  P
Sbjct: 575  YDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEADDDGDTP 634

Query: 601  FQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETV 660
             QRFTSE GVEN+RGR+LGG +M+NA F+S     F   +GV WD + VE AY+WV E++
Sbjct: 635  AQRFTSE-GVENVRGRVLGGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYEWVRESI 694

Query: 661  VSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAE 720
            VS   ++ WQ A K ALLEAGVGPDNG    H IGTK  GS FD  G RHGAVELLNK +
Sbjct: 695  VSFSNMSTWQFAVKEALLEAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVELLNKGD 754

Query: 721  PKNLKVAVNATVRKILF------NGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGS 780
             +N+K+A++A V K++F      +  SA GV Y+DSKGK H A IR KGE+ LSAGA+GS
Sbjct: 755  LQNMKIAIHAYVNKVIFSTKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILSAGALGS 814

Query: 781  PLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTL 840
            P LLL SG+GPKS+LSS  +P++H Q  VG+FM+DNPR    +++PF L  S+V+VVG  
Sbjct: 815  PQLLLLSGIGPKSYLSSQHIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAVQVVGIT 874

Query: 841  QDNVHLQAFASPAPFLAPPT--FSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTD 900
             D   ++  +   PF AP T  FS  P   T    SL     K     S G L L ST D
Sbjct: 875  ND-YFIETVSYSLPF-APTTLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTLASTKD 934

Query: 901  AKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPE 960
             K    VRFNY+S+P DLARCV  +RK+G++L+T+++++ K +DL G + F F+G  LP 
Sbjct: 935  VKITPHVRFNYFSNPIDLARCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLGPTLPS 994

Query: 961  NL-GDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTN 1020
            N   ++S +E+YCR +VTT WHYHGGCLVGKVVDGD++VIG  +LRVVDGSTF  SPGTN
Sbjct: 995  NYQSNNSGMEDYCRSSVTTIWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTN 1054

Query: 1021 PMATLMMLGRYVGLKVLQE-------------------RSNISYMKFVHDGNDFPEKEEY 1080
            P ATLMM+GRY+      E                     + SYMK V +  + P  EEY
Sbjct: 1055 PQATLMMIGRYLSWAKDAEGEDQLNLDFYEIYFTHTHLEMDFSYMKSVVNATNLPLIEEY 1114

Query: 1081 DYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKN--- 1140
            DYI++GGGT GCP+AATLS+N+S L++ERG+ P  +P+ L     +N+ + ED   N   
Sbjct: 1115 DYIVIGGGTTGCPIAATLSENYSVLVLERGNVPKAHPNTLVSSGFVNLLSEEDTDDNTNT 1174

Query: 1141 PYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVE 1200
            P  RFTSK+GV N+RGRVLGG+SMIN G +S+   DFF    GV K+DM  VEKAYKWVE
Sbjct: 1175 PAQRFTSKDGVDNVRGRVLGGSSMINAGFFSEAEHDFFVKYSGV-KWDMNEVEKAYKWVE 1234

Query: 1201 EALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGA----- 1260
            +++V   +L+ WQ A +  L+E G+ PDNG   +  VGTKI+GSIFD+ G RHGA     
Sbjct: 1235 DSIVSYSNLSTWQFAVKEALLEAGVGPDNGVTTKHKVGTKISGSIFDDEGRRHGAVELLN 1294

Query: 1261 ----------------------------------------------------IILSAGAI 1320
                                                                +ILSAG +
Sbjct: 1295 NGNLKNLQIAILAYVTKIIFSTNSSSHPNAIGVIYTDTKGKSHKALIRNKGEVILSAGPL 1354

Query: 1321 GSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIG 1380
            GSPQLLLLSG+GPKS+L+S +IP+IL  P+VG+FM DNPR  +  ++PFPL+ +  +++G
Sbjct: 1355 GSPQLLLLSGIGPKSNLTSQHIPIILSQPNVGQFMADNPRNNLNLVIPFPLEASYIQIVG 1414

Query: 1381 ISNNNTYYFQSIATTSPF---SIPPSFSLLPPNSTSVNLTVATIGGKFSKLTSVGSLRLN 1430
            I+ +  YY ++++ + PF   ++P  FS+ P  S  + L++ +I  K     S GSL L 
Sbjct: 1415 ITKD--YYIETMSYSLPFFPTTLP--FSIYPNTSNPLELSLGSIAEKIVGPLSTGSLWLA 1474

BLAST of Carg08367 vs. NCBI nr
Match: KAF4368137.1 (hypothetical protein G4B88_001041 [Cannabis sativa])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 677/1515 (44.69%), Postives = 906/1515 (59.80%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60
            MK V N   LP  EEYDYI+IGGGT GCP+AATLS N+SVLVLERG+ P A P+ L   G
Sbjct: 5    MKSVVNATNLPLIEEYDYIVIGGGTTGCPIAATLSENYSVLVLERGNVPKAHPNTLVSSG 64

Query: 61   LANTLT--DNDDGLN-PFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
              N L+  D DD  N P QRF S+DGV+N+                              
Sbjct: 65   FVNLLSEEDTDDNTNTPAQRFTSKDGVDNV------------------------------ 124

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
                                                                        
Sbjct: 125  ------------------------------------------------------------ 184

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFS--QSNGLSASGVLYSDFKGKLHKAT 240
              RGRRHGAVELLN  + +NL++A  A V +IIFS   S+  +A GV+Y+D KGK HKA 
Sbjct: 185  --RGRRHGAVELLNNGNLKNLQIAILAYVTKIIFSTNSSSHPNAIGVIYTDSKGKSHKAL 244

Query: 241  ISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIV 300
            I   GE+IL+AG +GSP LLL SG+GPKS+L+S  +P++L   +VGQ MADNPR    +V
Sbjct: 245  IRNKGEVILSAGPLGSPQLLLLSGIGPKSNLTSQHIPIILSQPNVGQFMADNPRNNLNLV 304

Query: 301  LPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP---PFGLLPPRSTAVNLSLAVFAGK 360
            +PF    + +Q+VG + ++ +IE++S  LPF  FP   PF + P  S  + LSL   A K
Sbjct: 305  IPFPLQASYIQIVG-ITKDYYIETMSYSLPF--FPTTLPFSIYPNTSNPLELSLGSIAEK 364

Query: 361  FSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIK-T 420
                 S GSL L    D +  P V+FNY S+P D+ RCV  +RK+GDL+ TKS++R K T
Sbjct: 365  IVGPLSTGSLWLASTTDVKVTPHVQFNYFSNPIDLTRCVSAMRKLGDLLKTKSLDRFKYT 424

Query: 421  GDLEGKKGFKFLGAPLPEN-MSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVI-- 480
             +L G + F F G PLP+N  +D   + ++CR +VT+FWHYHGGCLVGKVVD ++++   
Sbjct: 425  NNLNGTRDFTFFGPPLPDNYQTDNSSMEEYCRSSVTSFWHYHGGCLVGKVVDGDFKLSWS 484

Query: 481  ------GIKKLRVVDGST--------FSLSPGTNPMATVMMLGRYVGL--KMLQQRL--- 540
                     KL++ +  +        F  S        V+    +  +   +L  ++   
Sbjct: 485  ENVKRKNTAKLKISNVHSIQQYYIHLFVASKFKEINKNVLTSSYFANIIKNLLSWKITCS 544

Query: 541  -DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSV 600
             + SYMK V+NA DLP  +EYDYI+IGGG AGCPLAATLS K+ +L+LERG+ P  +P+V
Sbjct: 545  RNFSYMKSVYNAIDLPLVEEYDYIVIGGGTAGCPLAATLSEKYSILVLERGNTPKAHPNV 604

Query: 601  LNEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAG 660
            L+  G L  F+ +D+G  P QRFTSEDGV+NIRGR+LGG +MVN GF+S     FF  +G
Sbjct: 605  LSLSGFLKNFIEEDNGDTPAQRFTSEDGVDNIRGRVLGGSSMVNGGFFSEADDDFFAKSG 664

Query: 661  VNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGS 720
            V WDM  V+ AY+WV+  +VS   L+ WQS  K ALLE G+GPDN     + IGTK  GS
Sbjct: 665  VEWDMNEVQKAYKWVKNKIVSYSNLSIWQSVVKQALLEVGIGPDNKVTTKYKIGTKQSGS 724

Query: 721  IFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILFNGLSANGVSYSDSKGKIHTAFIRK 780
            IFD  G RHGAVELLNK   KNL++A++A V +           S S  K +        
Sbjct: 725  IFDNMGRRHGAVELLNKGNLKNLRIAIHAYVSQ-----------SISTQKRR-------- 784

Query: 781  KGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPF 840
                     A+GSP LLL SG+GPKS+LSS  +P+V  QP VG+FM+DNPR    ++ PF
Sbjct: 785  --------SALGSPQLLLLSGIGPKSYLSSQHIPIVLSQPNVGKFMADNPRNNLNLITPF 844

Query: 841  QLASSSVKVVG--------TLQDNVHLQAFASPAPFLAPPTFSL-LPPQATSINPSLVIF 900
             L SS ++VVG        TL  N+       P+ F  P  +SL +PP    +  S+   
Sbjct: 845  PLESSILQVVGITKEYYIETLSYNLPFSPTTLPSSFF-PHNYSLAIPP----LQLSVANI 904

Query: 901  VGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKI 960
            VGK +   S+G L L S +D K    VRFNY+S+P DL RCV  + K  DLLKT++++++
Sbjct: 905  VGKVAGPLSKGSLWLASNSDVKATPHVRFNYFSNPIDLERCVSMMGKFRDLLKTKSLDRM 964

Query: 961  KTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIG 1020
            K  DL+GN+ F F G  LP N    S ++ YCR +VTT+WHYHGGC VGKVVDGD++VIG
Sbjct: 965  KYNDLKGNRDFLFYGPSLPTNQTTISVIKHYCRSSVTTFWHYHGGCQVGKVVDGDFKVIG 1024

Query: 1021 MKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQE-----RSNISYMKFVHDGNDFP 1080
            + +LRVVDGSTF  SPGTNP A++MM+GRY+GLK+++E     R+ ++YMK V++  D P
Sbjct: 1025 IDSLRVVDGSTFISSPGTNPQASIMMIGRYLGLKMIRERQPKTRNLVNYMKSVYNATDLP 1084

Query: 1081 EKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDG 1140
              E+YDYI++GGGTAGCPLAATLS+ +S L++ERG+ P  +P+ L     L     EDDG
Sbjct: 1085 LVEKYDYIVIGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVSSGFLANLIEEDDG 1144

Query: 1141 KNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKW 1200
              P  RFTS++GV+N+RGRVLGG+SMIN   +S+ + DF   + GV+ +DM+ VEKAY+W
Sbjct: 1145 TTPAQRFTSEDGVENVRGRVLGGSSMINAAFFSEADNDFL-AKSGVE-WDMDAVEKAYEW 1204

Query: 1201 VEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGA--- 1260
            V+  +V   +L+ WQ A +   VE GI PDNG   +  +G K +GS FD+ G RHGA   
Sbjct: 1205 VKNKIVSYSNLSFWQAAAKEAFVEAGIGPDNGVTTKHKIGAKQSGSTFDDMGRRHGAVEL 1264

Query: 1261 ---------------------------IILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLI 1320
                                       +ILSAGA+GSPQLLLLSG+GPKS+LSS NIP++
Sbjct: 1265 LNNGNLNNLKIAIEASSHKALIRDKGEVILSAGALGSPQLLLLSGIGPKSYLSSQNIPIV 1324

Query: 1321 LHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIGISNNNTYYFQSIATTSPFS---IPP 1380
            L  P+VG+FM DNPR  +  I+PFPL+ ++ +V+GI+ +  YY ++ + + PFS   +P 
Sbjct: 1325 LSQPNVGKFMADNPRNNLNLIIPFPLEASTGQVVGITKD--YYIETNSYSLPFSPTKLPF 1383

Query: 1381 SF---SLLPPNSTSVNLTVATIGGKFSKLTSVGSLRLNSSTDVKTSPIVRFNYYSNPADI 1430
             F    LLPP      LT+     K     S G+L L S+TDVK +P VRFNY+SNP D+
Sbjct: 1385 GFYPTPLLPP-----QLTLTVFVEKIVGPLSTGALTLASATDVKVTPHVRFNYFSNPIDL 1383

BLAST of Carg08367 vs. NCBI nr
Match: KAF4368106.1 (hypothetical protein G4B88_001010 [Cannabis sativa])

HSP 1 Score: 1070.8 bits (2768), Expect = 9.9e-309
Identity = 558/1049 (53.19%), Postives = 714/1049 (68.06%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREE-YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQ 60
            MK V +   +   EE YDYIIIGGGTAGCPLAATLS N+SVLVLERGS P A P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   G-LANTLTDNDD-GLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
            G  AN + + ++  + P QRF SEDGVEN RGRVLGG SMIN GF+SR    FF   G++
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
            W+M  VEKAY+W+EE++V RP+L  WQS+FR+ALLE GVGPDN +DL H +GT+  GS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS-----------------AS 240
            D  GRRHGAVELLNK +  NL+V   ATV++I FS     +                 A 
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHV 300
            GV+YSD KGK H   +   GE+IL+AGAIGSP LLL SG+GP S+LSSL +P+V    +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP-PFGLLPPRS 360
            G  +ADNPR    +++P  T P+ VQVVG +  +  IE++S+ LP S+ P PF + P  S
Sbjct: 343  GDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPFSVYPKSS 402

Query: 361  TAVNLSLAVFAGKFSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGD 420
            TA +L + V   K     S GSL L    D R  P VRFNY SHP D+ +CV GVRK+G+
Sbjct: 403  TA-HLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGK 480
            ++ T +MER K  D   ++ F + G  LP N SD   + +FCR +VTT WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQR---LDVSYMKF 540
            VVD ++RV+G+  LRVVDGSTF +SPGTNP ATVMMLGRYVGLKMLQ+R    +V YMK 
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEYMKS 582

Query: 541  VHNAGDLP-EKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLL 600
            V++A ++  E++ YDYIIIG   AGCPLAATLS  + VL+LERGS P   P+VL+  G L
Sbjct: 583  VYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFL 642

Query: 601  NAFLAQDDGQ--NPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDM 660
               + +++     P QRFTSEDGVEN+RGR+LGG +M+NAGF+SRG   F+  +GV W+M
Sbjct: 643  ANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEM 702

Query: 661  EMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGK 720
            + VE AY+WVEE++V +P L  WQS+F+ ALLE GVGP+NGF+L H +GTK  GS FD  
Sbjct: 703  DRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEV 762

Query: 721  GNRHGAVELLNKAEPKNLKVAVNATVRKILFNGLSANGVSYSD-----SKGKIHTAFIRK 780
            G RHGAVELLNK    NLKV V+ATV +I+F+   +N     D     S  K HT  +R 
Sbjct: 763  GRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPRKSHTVLVRH 822

Query: 781  KGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPF 840
            KGEI LSAGAIGSP LLL SG+GP S+LSSL +P+V  QP VG+F++DNPR    +++PF
Sbjct: 823  KGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNINLIIPF 882

Query: 841  QLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKFSEVHS 900
                S+V+VVG + D+  ++  ++  P    P    + P++++ N  + I   K     S
Sbjct: 883  PTEPSAVQVVG-ITDHYFMETISANLPNSPTPLPFSMYPKSSTANLGMAIICEKLRHPLS 942

Query: 901  EGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLEGNK 960
             G L L S+ D +    VRFNY+SHP DL++CV  VRK+G++L +++ME+ K  D    +
Sbjct: 943  SGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVDSNEER 1002

Query: 961  GFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRVVDG 1014
             F + G  LP N  D+S++EE+CR +VTT WHYHGGC VGKVVDGD+RV G+ +LRVVDG
Sbjct: 1003 NFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNSLRVVDG 1062

BLAST of Carg08367 vs. NCBI nr
Match: KAF4349168.1 (hypothetical protein F8388_026317 [Cannabis sativa])

HSP 1 Score: 1066.6 bits (2757), Expect = 1.8e-307
Identity = 567/1115 (50.85%), Postives = 722/1115 (64.75%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREE-YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQ 60
            MK V +   +   EE YDYIIIGGGTAGCPLAATLS N+SVLVLERGS P A P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   G-LANTLTDNDD-GLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
            G  AN + + ++  + P QRF SEDGVEN RGRVLGG SMIN GF+SR    FF   G++
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
            W+M  VEKAY+W+EE++V RP+L  WQS+FR+ALLE GVGPDN +DL H +GT+  GS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS-----------------AS 240
            D  GRRHGAVELLNK +  NL+V   ATV++I FS     +                 A 
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHV 300
            GV+YSD KGK H   +   GE+IL+AGAIGSP LLL SG+GP S+LSSL +P+V    +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP-PFGLLPPRS 360
            G  +ADNPR    +++P  T P+ VQVVG +  +  IE++S+ LP S+ P PF + P  S
Sbjct: 343  GDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPFSVYPKSS 402

Query: 361  TAVNLSLAVFAGKFSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGD 420
            TA +L + V   K     S GSL L    D R  P VRFNY SHP D+ +CV GVRK+G+
Sbjct: 403  TA-HLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGK 480
            ++ T +MER K  D   ++ F + G  LP N SD   + +FCR +VTT WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQR----------- 540
            VVD ++RV+G+  LRVVDGSTF +SPGTNP ATVMMLGRYVGLKMLQ+R           
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEGDIE 582

Query: 541  ------------------------------------------LDVSYMKFVHNAGDLP-E 600
                                                       D  YMK V++A ++  E
Sbjct: 583  RREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYDATEMSLE 642

Query: 601  KQEYDYIIIGGG--AAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDD 660
            ++ YDYIIIGGG   AGCPLAATLS  + VL+LERGS P   P+VL+  G L   + +++
Sbjct: 643  EEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLANLMQEEE 702

Query: 661  GQ--NPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQ 720
                 P QRFTSEDGVEN+RGR+LGG +M+NAGF+SRG   F+  +GV W+M+ VE AY+
Sbjct: 703  ETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYE 762

Query: 721  WVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVE 780
            WVEE++V +P L  WQS+F+ ALLE GVGP+NGF+L H +GTK  GS FD  G RHGAVE
Sbjct: 763  WVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVGRRHGAVE 822

Query: 781  LLNKAEPKNLKVAVNATVRKILFNGLSAN------------------GVSYSDSKGKIHT 840
            LLNK    NLKV V+ATV +I+F+   +N                  GV YSDSKGK HT
Sbjct: 823  LLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSDSKGKSHT 882

Query: 841  AFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGAT 900
              +R KGEI LSAGAIGSP LLL SG+GP S+LSSL +P+V  QP VG+F++DNPR    
Sbjct: 883  VLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNIN 942

Query: 901  IVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKF 960
            +++PF    S+V+VVG + D+  ++  ++  P    P    + P++++ N  + I   K 
Sbjct: 943  LIIPFPTEPSAVQVVG-ITDHYFMETISANLPNSPTPLPFSMYPKSSTANLGMAIICEKL 1002

Query: 961  SEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQD 1015
                S G L L S+ D +    VRFNY+SHP DL++CV  VRK+G++L +++ME+ K  D
Sbjct: 1003 RHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVD 1062

BLAST of Carg08367 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.5e-150
Identity = 260/514 (50.58%), Postives = 354/514 (68.87%), Query Frame = 0

Query: 506  DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVL 565
            D SY++F ++A DL  +  YDY+I+GGG +GCPLAATLS K+KVL+LERGS P  YP+VL
Sbjct: 35   DFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 566  NEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGV 625
               G +     +DDG+ P +RF SEDG++N+RGR+LGG +M+NAG Y+R +   +  +GV
Sbjct: 95   TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGV 154

Query: 626  NWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSI 685
            +WDM++V   Y+WVE+T+V KP    WQS   +A LEAGV P++GF+L H  GT+  GS 
Sbjct: 155  DWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGST 214

Query: 686  FDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILFN---GLSANGVSYSDSKGKIHTAFI 745
            FD KG RH A ELLNK    NL+V V+A+V KI+F+   GL+A GV Y DS G  H AF+
Sbjct: 215  FDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFV 274

Query: 746  RKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVL 805
            R KGE+ +SAG IG+P LLL SGVGP+S+LSSL +PVV   PYVG+F+ DNPR    I+ 
Sbjct: 275  RSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILP 334

Query: 806  PFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSI-NPSLVIFVGKFSE 865
            P  +  + V V+G   D    Q   S  PF  PP FS  P  +  + N +   F  K + 
Sbjct: 335  PNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAG 394

Query: 866  VHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLE 925
              S G L L S+++ + +  V+FNYYS+P DL+ CV G++K+G+LL T  ++  K +DL 
Sbjct: 395  PLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLP 454

Query: 926  GNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRV 985
            G +GF  +G+PLP++  DD++ E +CR++V +YWHYHGGCLVGKV+DGD+RV G+  LRV
Sbjct: 455  GIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRV 514

Query: 986  VDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERS 1016
            VDGSTF  +P ++P    +MLGRYVG+K+LQERS
Sbjct: 515  VDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545

BLAST of Carg08367 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 6.3e-149
Identity = 258/514 (50.19%), Postives = 353/514 (68.68%), Query Frame = 0

Query: 506  DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVL 565
            D SY+ F ++A DL  +  YDY+I+GGG +GCPLAATLS K+KVL+LERGS P  YP+VL
Sbjct: 35   DFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 566  NEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGV 625
               G +     +DDG+ P +RF SEDG++N+RGR+LGG +++NAG Y+R +   +  +GV
Sbjct: 95   TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGV 154

Query: 626  NWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSI 685
            +WDM++V   Y+WVE+T+V KP   +WQS  K+A LEAGV P++GF+L H  GT+  GS 
Sbjct: 155  DWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGST 214

Query: 686  FDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILFN---GLSANGVSYSDSKGKIHTAFI 745
            FD KG RH A ELLNK    NL+V V+A+V KI+F+   GL+A GV Y DS G  H AF+
Sbjct: 215  FDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFV 274

Query: 746  RKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVL 805
            R KGE+ +SAG IG+P LLL SGVGP+S+LSSL +PVV   PYVG+F+ DNPR    I+ 
Sbjct: 275  RSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILP 334

Query: 806  PFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSI-NPSLVIFVGKFSE 865
            P  +  + V V+G   D    Q   S  PF  PP F   P  +  + N +   F  K + 
Sbjct: 335  PNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAG 394

Query: 866  VHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLE 925
              S G L L S+++ + +  V+FNYYS+  DL+ CV G++K+G+LL T  ++  K +DL 
Sbjct: 395  PLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLP 454

Query: 926  GNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRV 985
            G +GF  +G+PLP++  DD++ E +CR++V +YWHYHGGCLVGKV+DGD+RV G+  LRV
Sbjct: 455  GVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRV 514

Query: 986  VDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERS 1016
            VDGSTF  +P ++P    +MLGRYVG+K+LQERS
Sbjct: 515  VDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545

BLAST of Carg08367 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.2e-146
Identity = 254/515 (49.32%), Postives = 349/515 (67.77%), Query Frame = 0

Query: 506  DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVL 565
            D SY+ FV++A D   +  YDYII+GGG AGCPLAATLS+ + VL+LERGS P +YP++L
Sbjct: 35   DFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLL 94

Query: 566  NEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGV 625
               G +     +DDG+ P +RF SEDG++N+RGR+LGG +M+NAG Y R +  FF   G+
Sbjct: 95   ISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGI 154

Query: 626  NWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSI 685
             WDM++V   Y+WVE+T+V +P    WQ+   +A LEAG+ P+NGF++ HL GT+  GS 
Sbjct: 155  EWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGST 214

Query: 686  FDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILF----NGLSANGVSYSDSKGKIHTAF 745
            FD  G RH + ELLNK +P NL+VAV A V KI+F    +G++A GV Y+DS G  H AF
Sbjct: 215  FDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAF 274

Query: 746  IRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIV 805
            +R +GE+ LSAG IGSP LLL SGVGP+S+L+SL + VV   PYVG+++ DNPR    I+
Sbjct: 275  VRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINIL 334

Query: 806  LPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSI-NPSLVIFVGKFS 865
             P  + +S+V V+G   D    Q   S  PF  PP FS  P  +  + N +    V K  
Sbjct: 335  PPNPIEASTVTVLGITSD--FYQCSISSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVP 394

Query: 866  EVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDL 925
               S G + LNS++D +    V+FNYYS+  DL+ CV G++K+G++L T  +E  K +DL
Sbjct: 395  GPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDL 454

Query: 926  EGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLR 985
             G  GF  +G+PLPEN  DD++ E +CR++V +YWHYHGGCLVGKV+D  +RV G+  LR
Sbjct: 455  PGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALR 514

Query: 986  VVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERS 1016
            VVDGSTF  +P ++P    +MLGRY+G+++LQERS
Sbjct: 515  VVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERS 546

BLAST of Carg08367 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 7.2e-145
Identity = 260/515 (50.49%), Postives = 341/515 (66.21%), Query Frame = 0

Query: 506  DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVL 565
            D SY+KFV+NA D   +  YDYI+IGGG +GCPLAATLS K+KVLLLERG+   +YP+ L
Sbjct: 35   DFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPNTL 94

Query: 566  NEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGV 625
               G       QDDG+ P +RF SEDG++N+R RILGG T++NAG Y+R +  F+   G+
Sbjct: 95   TADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTIINAGVYARANISFYSQTGI 154

Query: 626  NWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSI 685
             WD+++V   Y+WVE+ +V KP   +WQS      LEAG+ PDNGF+L H  GT+  GS 
Sbjct: 155  EWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGST 214

Query: 686  FDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILF----NGLSANGVSYSDSKGKIHTAF 745
            FD  G RH A ELLNK +P NL VAV A+V KILF    + LSA GV Y+DS G  H AF
Sbjct: 215  FDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAF 274

Query: 746  IRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIV 805
            +R  GE+ +SAG IG+P LLL SGVGP+S+LSSL + VV   PYVG+F+ +NPR      
Sbjct: 275  VRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNF 334

Query: 806  LPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSI-NPSLVIFVGKFS 865
             P  + +S V V+G   D  + Q   S  PF  PP FSL P  +  + N +    V +  
Sbjct: 335  PPNPIEASVVTVLGIRSD--YYQVSLSSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVP 394

Query: 866  EVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDL 925
               S G + LNS++D +    ++FNYYS+  DLA CV G++K+GDLL+T+ +E  K +D+
Sbjct: 395  GPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDV 454

Query: 926  EGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLR 985
             G  GF ++GVPLPEN  DD+S E +C   V +YWHYHGG LVGKV+D  +RV+G+K LR
Sbjct: 455  LGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALR 514

Query: 986  VVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERS 1016
            VVD STF   P ++P    +MLGRYVGL++LQERS
Sbjct: 515  VVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

BLAST of Carg08367 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-144
Identity = 251/514 (48.83%), Postives = 346/514 (67.32%), Query Frame = 0

Query: 509  YMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQ 568
            +M+F+ NA D   +  YDYII+GGG AGCPLAATLS  F+VLLLERG  P   P+V++  
Sbjct: 38   FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 569  GLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWD 628
            G L      ++  +P Q F SE+GV N RGR+LGG + +NAGFYSR  +QFFE +G+ WD
Sbjct: 98   GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 629  MEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDG 688
            +  V  +Y+WVE  +V +P L  WQ+A + ALLE GV P NGF L H +GTK GGS FD 
Sbjct: 158  LSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDR 217

Query: 689  KGNRHGAVELLNKAEPKNLKVAVNATVRKILF--------NGLSANGVSYSDSKGKIHTA 748
             G RH + +LL  A   N++VAV ATV ++L         + +SA GV Y D  G+ H A
Sbjct: 218  TGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHA 277

Query: 749  FIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATI 808
             IR +GE+ LSAGA+GSP LL  SG+GP+S+LS+  +PV   QP+VG+F+ DNPR G +I
Sbjct: 278  LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 809  VLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKFS 868
            V P  + +S ++VVG  +D   L+A ++  PF +P     +   A+ +   +   + K  
Sbjct: 338  VPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKIL 397

Query: 869  EVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDL 928
               S G LRL ++TD + N +VRFNY+S P DL RCV G RK+G++L+++ M+    ++ 
Sbjct: 398  GPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 457

Query: 929  EGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLR 988
             GN+ F+F+G PLP +  +D  + ++CR+TV+T WHYHGG +VGKVVD D +VIG+ +LR
Sbjct: 458  FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 517

Query: 989  VVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQER 1015
            +VDGSTF+ SPGTNP ATLMMLGRY+GLK+L+ER
Sbjct: 518  LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Carg08367 vs. ExPASy TrEMBL
Match: A0A7J6GLR3 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016288 PE=3 SV=1)

HSP 1 Score: 1461.8 bits (3783), Expect = 0.0e+00
Identity = 775/1539 (50.36%), Postives = 1004/1539 (65.24%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60
            MK V N   +P  EEYDYI++GGGTAGCPLAATLS  +SVLVLERG+ P A P+ L   G
Sbjct: 35   MKSVYNATDMPLEEEYDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASG 94

Query: 61   LANTL--TDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQW 120
                L   D+DDG  P QRF SE+GVE++RGRVLGG SMIN  F+S A  +F   SG++W
Sbjct: 95   FLTNLVEADDDDGDTPAQRFTSEEGVESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEW 154

Query: 121  DMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFD 180
            D   VEKAY+W++  +VS   LS WQSA +EALLEAGVGPDNG   KH VGT+  GS FD
Sbjct: 155  DNDEVEKAYEWVKSKIVSYSNLSTWQSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFD 214

Query: 181  SRGRRHGAVELLNKADPRNLRVATQATVKRIIFS-QSNGLSASGVLYSDFKGKLHKATIS 240
            + GRRHGAVELLNK + +N+++A  A VK+IIFS +S+  SA GV+Y+D KGK HKA I 
Sbjct: 215  NMGRRHGAVELLNKGNLKNMKIAIHAYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIR 274

Query: 241  KNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLP 300
              GE+IL+AGA+GSP LLL SG+GPKS+LSS  +P+V    +VG+ MADNPR    +++P
Sbjct: 275  NKGEVILSAGALGSPQLLLLSGIGPKSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIP 334

Query: 301  FLTPPTSVQVVGTLKRNIHIESLSSILPFS-IFPPFGLLPPRSTAVNLSLAVFAGKFSTV 360
            F    +  QVVG +  +  IE++S  LPF+    PF   P   T    SLA    K    
Sbjct: 335  FPFEGSGAQVVG-ITDDYFIETVSYSLPFAPTTLPFSFYPNPLTPPQFSLASIVEKIVGP 394

Query: 361  SSVGSLRLDGRKN----PIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEG 420
             S GSL L   K+    P VRFNY S+P D+ RCV  +RKVG+++ TKS++R K  DL G
Sbjct: 395  LSTGSLTLASTKDVKITPHVRFNYFSNPIDLARCVSAMRKVGEMLETKSLDRFKYKDLNG 454

Query: 421  KKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVV 480
             + F +LG PLP N SD   + D+CR +VTTFWHYHGGCLVGKVVD +++VIG   LRVV
Sbjct: 455  ARDFMYLGPPLPANQSDNSAMEDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGTNSLRVV 514

Query: 481  DGSTFSLSPGTNPMATVMMLGRY--------VGLKMLQQRLDVSYMKFVHNAGDLPEKQE 540
            DGSTF +SPGTNP AT+MM+GR         +G+ +     D SYMK V+NA D+P ++E
Sbjct: 515  DGSTFVISPGTNPQATLMMIGRVGDNNGPFGLGIAVSGADYDFSYMKSVYNATDMPLEEE 574

Query: 541  YDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLL-NAFLAQDDGQNP 600
            YDYI++GGG AGCPLAATLS K+ VL+LERG+ P  +P+ L   G L N   A DDG  P
Sbjct: 575  YDYIVVGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEADDDGDTP 634

Query: 601  FQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETV 660
             QRFTSE GVEN+RGR+LGG +M+NA F+S     F   +GV WD + VE AY+WV E++
Sbjct: 635  AQRFTSE-GVENVRGRVLGGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYEWVRESI 694

Query: 661  VSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAE 720
            VS   ++ WQ A K ALLEAGVGPDNG    H IGTK  GS FD  G RHGAVELLNK +
Sbjct: 695  VSFSNMSTWQFAVKEALLEAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVELLNKGD 754

Query: 721  PKNLKVAVNATVRKILF------NGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGS 780
             +N+K+A++A V K++F      +  SA GV Y+DSKGK H A IR KGE+ LSAGA+GS
Sbjct: 755  LQNMKIAIHAYVNKVIFSTKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILSAGALGS 814

Query: 781  PLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTL 840
            P LLL SG+GPKS+LSS  +P++H Q  VG+FM+DNPR    +++PF L  S+V+VVG  
Sbjct: 815  PQLLLLSGIGPKSYLSSQHIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAVQVVGIT 874

Query: 841  QDNVHLQAFASPAPFLAPPT--FSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTD 900
             D   ++  +   PF AP T  FS  P   T    SL     K     S G L L ST D
Sbjct: 875  ND-YFIETVSYSLPF-APTTLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTLASTKD 934

Query: 901  AKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPE 960
             K    VRFNY+S+P DLARCV  +RK+G++L+T+++++ K +DL G + F F+G  LP 
Sbjct: 935  VKITPHVRFNYFSNPIDLARCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLGPTLPS 994

Query: 961  NL-GDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTN 1020
            N   ++S +E+YCR +VTT WHYHGGCLVGKVVDGD++VIG  +LRVVDGSTF  SPGTN
Sbjct: 995  NYQSNNSGMEDYCRSSVTTIWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTN 1054

Query: 1021 PMATLMMLGRYVGLKVLQE-------------------RSNISYMKFVHDGNDFPEKEEY 1080
            P ATLMM+GRY+      E                     + SYMK V +  + P  EEY
Sbjct: 1055 PQATLMMIGRYLSWAKDAEGEDQLNLDFYEIYFTHTHLEMDFSYMKSVVNATNLPLIEEY 1114

Query: 1081 DYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKN--- 1140
            DYI++GGGT GCP+AATLS+N+S L++ERG+ P  +P+ L     +N+ + ED   N   
Sbjct: 1115 DYIVIGGGTTGCPIAATLSENYSVLVLERGNVPKAHPNTLVSSGFVNLLSEEDTDDNTNT 1174

Query: 1141 PYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVE 1200
            P  RFTSK+GV N+RGRVLGG+SMIN G +S+   DFF    GV K+DM  VEKAYKWVE
Sbjct: 1175 PAQRFTSKDGVDNVRGRVLGGSSMINAGFFSEAEHDFFVKYSGV-KWDMNEVEKAYKWVE 1234

Query: 1201 EALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGA----- 1260
            +++V   +L+ WQ A +  L+E G+ PDNG   +  VGTKI+GSIFD+ G RHGA     
Sbjct: 1235 DSIVSYSNLSTWQFAVKEALLEAGVGPDNGVTTKHKVGTKISGSIFDDEGRRHGAVELLN 1294

Query: 1261 ----------------------------------------------------IILSAGAI 1320
                                                                +ILSAG +
Sbjct: 1295 NGNLKNLQIAILAYVTKIIFSTNSSSHPNAIGVIYTDTKGKSHKALIRNKGEVILSAGPL 1354

Query: 1321 GSPQLLLLSGVGPKSHLSSLNIPLILHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIG 1380
            GSPQLLLLSG+GPKS+L+S +IP+IL  P+VG+FM DNPR  +  ++PFPL+ +  +++G
Sbjct: 1355 GSPQLLLLSGIGPKSNLTSQHIPIILSQPNVGQFMADNPRNNLNLVIPFPLEASYIQIVG 1414

Query: 1381 ISNNNTYYFQSIATTSPF---SIPPSFSLLPPNSTSVNLTVATIGGKFSKLTSVGSLRLN 1430
            I+ +  YY ++++ + PF   ++P  FS+ P  S  + L++ +I  K     S GSL L 
Sbjct: 1415 ITKD--YYIETMSYSLPFFPTTLP--FSIYPNTSNPLELSLGSIAEKIVGPLSTGSLWLA 1474

BLAST of Carg08367 vs. ExPASy TrEMBL
Match: A0A7J6FBQ5 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001041 PE=3 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 677/1515 (44.69%), Postives = 906/1515 (59.80%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60
            MK V N   LP  EEYDYI+IGGGT GCP+AATLS N+SVLVLERG+ P A P+ L   G
Sbjct: 5    MKSVVNATNLPLIEEYDYIVIGGGTTGCPIAATLSENYSVLVLERGNVPKAHPNTLVSSG 64

Query: 61   LANTLT--DNDDGLN-PFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
              N L+  D DD  N P QRF S+DGV+N+                              
Sbjct: 65   FVNLLSEEDTDDNTNTPAQRFTSKDGVDNV------------------------------ 124

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
                                                                        
Sbjct: 125  ------------------------------------------------------------ 184

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFS--QSNGLSASGVLYSDFKGKLHKAT 240
              RGRRHGAVELLN  + +NL++A  A V +IIFS   S+  +A GV+Y+D KGK HKA 
Sbjct: 185  --RGRRHGAVELLNNGNLKNLQIAILAYVTKIIFSTNSSSHPNAIGVIYTDSKGKSHKAL 244

Query: 241  ISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIV 300
            I   GE+IL+AG +GSP LLL SG+GPKS+L+S  +P++L   +VGQ MADNPR    +V
Sbjct: 245  IRNKGEVILSAGPLGSPQLLLLSGIGPKSNLTSQHIPIILSQPNVGQFMADNPRNNLNLV 304

Query: 301  LPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP---PFGLLPPRSTAVNLSLAVFAGK 360
            +PF    + +Q+VG + ++ +IE++S  LPF  FP   PF + P  S  + LSL   A K
Sbjct: 305  IPFPLQASYIQIVG-ITKDYYIETMSYSLPF--FPTTLPFSIYPNTSNPLELSLGSIAEK 364

Query: 361  FSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIK-T 420
                 S GSL L    D +  P V+FNY S+P D+ RCV  +RK+GDL+ TKS++R K T
Sbjct: 365  IVGPLSTGSLWLASTTDVKVTPHVQFNYFSNPIDLTRCVSAMRKLGDLLKTKSLDRFKYT 424

Query: 421  GDLEGKKGFKFLGAPLPEN-MSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVI-- 480
             +L G + F F G PLP+N  +D   + ++CR +VT+FWHYHGGCLVGKVVD ++++   
Sbjct: 425  NNLNGTRDFTFFGPPLPDNYQTDNSSMEEYCRSSVTSFWHYHGGCLVGKVVDGDFKLSWS 484

Query: 481  ------GIKKLRVVDGST--------FSLSPGTNPMATVMMLGRYVGL--KMLQQRL--- 540
                     KL++ +  +        F  S        V+    +  +   +L  ++   
Sbjct: 485  ENVKRKNTAKLKISNVHSIQQYYIHLFVASKFKEINKNVLTSSYFANIIKNLLSWKITCS 544

Query: 541  -DVSYMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSV 600
             + SYMK V+NA DLP  +EYDYI+IGGG AGCPLAATLS K+ +L+LERG+ P  +P+V
Sbjct: 545  RNFSYMKSVYNAIDLPLVEEYDYIVIGGGTAGCPLAATLSEKYSILVLERGNTPKAHPNV 604

Query: 601  LNEQGLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAG 660
            L+  G L  F+ +D+G  P QRFTSEDGV+NIRGR+LGG +MVN GF+S     FF  +G
Sbjct: 605  LSLSGFLKNFIEEDNGDTPAQRFTSEDGVDNIRGRVLGGSSMVNGGFFSEADDDFFAKSG 664

Query: 661  VNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGS 720
            V WDM  V+ AY+WV+  +VS   L+ WQS  K ALLE G+GPDN     + IGTK  GS
Sbjct: 665  VEWDMNEVQKAYKWVKNKIVSYSNLSIWQSVVKQALLEVGIGPDNKVTTKYKIGTKQSGS 724

Query: 721  IFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILFNGLSANGVSYSDSKGKIHTAFIRK 780
            IFD  G RHGAVELLNK   KNL++A++A V +           S S  K +        
Sbjct: 725  IFDNMGRRHGAVELLNKGNLKNLRIAIHAYVSQ-----------SISTQKRR-------- 784

Query: 781  KGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPF 840
                     A+GSP LLL SG+GPKS+LSS  +P+V  QP VG+FM+DNPR    ++ PF
Sbjct: 785  --------SALGSPQLLLLSGIGPKSYLSSQHIPIVLSQPNVGKFMADNPRNNLNLITPF 844

Query: 841  QLASSSVKVVG--------TLQDNVHLQAFASPAPFLAPPTFSL-LPPQATSINPSLVIF 900
             L SS ++VVG        TL  N+       P+ F  P  +SL +PP    +  S+   
Sbjct: 845  PLESSILQVVGITKEYYIETLSYNLPFSPTTLPSSFF-PHNYSLAIPP----LQLSVANI 904

Query: 901  VGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKI 960
            VGK +   S+G L L S +D K    VRFNY+S+P DL RCV  + K  DLLKT++++++
Sbjct: 905  VGKVAGPLSKGSLWLASNSDVKATPHVRFNYFSNPIDLERCVSMMGKFRDLLKTKSLDRM 964

Query: 961  KTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIG 1020
            K  DL+GN+ F F G  LP N    S ++ YCR +VTT+WHYHGGC VGKVVDGD++VIG
Sbjct: 965  KYNDLKGNRDFLFYGPSLPTNQTTISVIKHYCRSSVTTFWHYHGGCQVGKVVDGDFKVIG 1024

Query: 1021 MKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQE-----RSNISYMKFVHDGNDFP 1080
            + +LRVVDGSTF  SPGTNP A++MM+GRY+GLK+++E     R+ ++YMK V++  D P
Sbjct: 1025 IDSLRVVDGSTFISSPGTNPQASIMMIGRYLGLKMIRERQPKTRNLVNYMKSVYNATDLP 1084

Query: 1081 EKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDG 1140
              E+YDYI++GGGTAGCPLAATLS+ +S L++ERG+ P  +P+ L     L     EDDG
Sbjct: 1085 LVEKYDYIVIGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTLVSSGFLANLIEEDDG 1144

Query: 1141 KNPYNRFTSKEGVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKW 1200
              P  RFTS++GV+N+RGRVLGG+SMIN   +S+ + DF   + GV+ +DM+ VEKAY+W
Sbjct: 1145 TTPAQRFTSEDGVENVRGRVLGGSSMINAAFFSEADNDFL-AKSGVE-WDMDAVEKAYEW 1204

Query: 1201 VEEALVYRPSLNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGA--- 1260
            V+  +V   +L+ WQ A +   VE GI PDNG   +  +G K +GS FD+ G RHGA   
Sbjct: 1205 VKNKIVSYSNLSFWQAAAKEAFVEAGIGPDNGVTTKHKIGAKQSGSTFDDMGRRHGAVEL 1264

Query: 1261 ---------------------------IILSAGAIGSPQLLLLSGVGPKSHLSSLNIPLI 1320
                                       +ILSAGA+GSPQLLLLSG+GPKS+LSS NIP++
Sbjct: 1265 LNNGNLNNLKIAIEASSHKALIRDKGEVILSAGALGSPQLLLLSGIGPKSYLSSQNIPIV 1324

Query: 1321 LHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIGISNNNTYYFQSIATTSPFS---IPP 1380
            L  P+VG+FM DNPR  +  I+PFPL+ ++ +V+GI+ +  YY ++ + + PFS   +P 
Sbjct: 1325 LSQPNVGKFMADNPRNNLNLIIPFPLEASTGQVVGITKD--YYIETNSYSLPFSPTKLPF 1383

Query: 1381 SF---SLLPPNSTSVNLTVATIGGKFSKLTSVGSLRLNSSTDVKTSPIVRFNYYSNPADI 1430
             F    LLPP      LT+     K     S G+L L S+TDVK +P VRFNY+SNP D+
Sbjct: 1385 GFYPTPLLPP-----QLTLTVFVEKIVGPLSTGALTLASATDVKVTPHVRFNYFSNPIDL 1383

BLAST of Carg08367 vs. ExPASy TrEMBL
Match: A0A7J6FBM4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001010 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 4.8e-309
Identity = 558/1049 (53.19%), Postives = 714/1049 (68.06%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREE-YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQ 60
            MK V +   +   EE YDYIIIGGGTAGCPLAATLS N+SVLVLERGS P A P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   G-LANTLTDNDD-GLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
            G  AN + + ++  + P QRF SEDGVEN RGRVLGG SMIN GF+SR    FF   G++
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
            W+M  VEKAY+W+EE++V RP+L  WQS+FR+ALLE GVGPDN +DL H +GT+  GS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS-----------------AS 240
            D  GRRHGAVELLNK +  NL+V   ATV++I FS     +                 A 
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHV 300
            GV+YSD KGK H   +   GE+IL+AGAIGSP LLL SG+GP S+LSSL +P+V    +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP-PFGLLPPRS 360
            G  +ADNPR    +++P  T P+ VQVVG +  +  IE++S+ LP S+ P PF + P  S
Sbjct: 343  GDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPFSVYPKSS 402

Query: 361  TAVNLSLAVFAGKFSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGD 420
            TA +L + V   K     S GSL L    D R  P VRFNY SHP D+ +CV GVRK+G+
Sbjct: 403  TA-HLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGK 480
            ++ T +MER K  D   ++ F + G  LP N SD   + +FCR +VTT WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQR---LDVSYMKF 540
            VVD ++RV+G+  LRVVDGSTF +SPGTNP ATVMMLGRYVGLKMLQ+R    +V YMK 
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEYMKS 582

Query: 541  VHNAGDLP-EKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLL 600
            V++A ++  E++ YDYIIIG   AGCPLAATLS  + VL+LERGS P   P+VL+  G L
Sbjct: 583  VYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFL 642

Query: 601  NAFLAQDDGQ--NPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDM 660
               + +++     P QRFTSEDGVEN+RGR+LGG +M+NAGF+SRG   F+  +GV W+M
Sbjct: 643  ANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEM 702

Query: 661  EMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGK 720
            + VE AY+WVEE++V +P L  WQS+F+ ALLE GVGP+NGF+L H +GTK  GS FD  
Sbjct: 703  DRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEV 762

Query: 721  GNRHGAVELLNKAEPKNLKVAVNATVRKILFNGLSANGVSYSD-----SKGKIHTAFIRK 780
            G RHGAVELLNK    NLKV V+ATV +I+F+   +N     D     S  K HT  +R 
Sbjct: 763  GRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPRKSHTVLVRH 822

Query: 781  KGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPF 840
            KGEI LSAGAIGSP LLL SG+GP S+LSSL +P+V  QP VG+F++DNPR    +++PF
Sbjct: 823  KGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNINLIIPF 882

Query: 841  QLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKFSEVHS 900
                S+V+VVG + D+  ++  ++  P    P    + P++++ N  + I   K     S
Sbjct: 883  PTEPSAVQVVG-ITDHYFMETISANLPNSPTPLPFSMYPKSSTANLGMAIICEKLRHPLS 942

Query: 901  EGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLEGNK 960
             G L L S+ D +    VRFNY+SHP DL++CV  VRK+G++L +++ME+ K  D    +
Sbjct: 943  SGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVDSNEER 1002

Query: 961  GFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRVVDG 1014
             F + G  LP N  D+S++EE+CR +VTT WHYHGGC VGKVVDGD+RV G+ +LRVVDG
Sbjct: 1003 NFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNSLRVVDG 1062

BLAST of Carg08367 vs. ExPASy TrEMBL
Match: A0A7J6DSU4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=F8388_026317 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 9.0e-308
Identity = 567/1115 (50.85%), Postives = 722/1115 (64.75%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREE-YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQ 60
            MK V +   +   EE YDYIIIGGGTAGCPLAATLS N+SVLVLERGS P A P+VL   
Sbjct: 43   MKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 102

Query: 61   G-LANTLTDNDD-GLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
            G  AN + + ++  + P QRF SEDGVEN RGRVLGG SMIN GF+SR    FF   G++
Sbjct: 103  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 162

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
            W+M  VEKAY+W+EE++V RP+L  WQS+FR+ALLE GVGPDN +DL H +GT+  GS F
Sbjct: 163  WEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 222

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS-----------------AS 240
            D  GRRHGAVELLNK +  NL+V   ATV++I FS     +                 A 
Sbjct: 223  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNNPPKPYAI 282

Query: 241  GVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHV 300
            GV+YSD KGK H   +   GE+IL+AGAIGSP LLL SG+GP S+LSSL +P+V    +V
Sbjct: 283  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 342

Query: 301  GQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP-PFGLLPPRS 360
            G  +ADNPR    +++P  T P+ VQVVG +  +  IE++S+ LP S+ P PF + P  S
Sbjct: 343  GDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPFSVYPKSS 402

Query: 361  TAVNLSLAVFAGKFSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGD 420
            TA +L + V   K     S GSL L    D R  P VRFNY SHP D+ +CV GVRK+G+
Sbjct: 403  TA-HLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 462

Query: 421  LVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGK 480
            ++ T +MER K  D   ++ F + G  LP N SD   + +FCR +VTT WHYHGGC VGK
Sbjct: 463  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 522

Query: 481  VVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQR----------- 540
            VVD ++RV+G+  LRVVDGSTF +SPGTNP ATVMMLGRYVGLKMLQ+R           
Sbjct: 523  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVEGDIE 582

Query: 541  ------------------------------------------LDVSYMKFVHNAGDLP-E 600
                                                       D  YMK V++A ++  E
Sbjct: 583  RREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYDATEMSLE 642

Query: 601  KQEYDYIIIGGG--AAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDD 660
            ++ YDYIIIGGG   AGCPLAATLS  + VL+LERGS P   P+VL+  G L   + +++
Sbjct: 643  EEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLANLMQEEE 702

Query: 661  GQ--NPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQ 720
                 P QRFTSEDGVEN+RGR+LGG +M+NAGF+SRG   F+  +GV W+M+ VE AY+
Sbjct: 703  ETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYE 762

Query: 721  WVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVE 780
            WVEE++V +P L  WQS+F+ ALLE GVGP+NGF+L H +GTK  GS FD  G RHGAVE
Sbjct: 763  WVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVGRRHGAVE 822

Query: 781  LLNKAEPKNLKVAVNATVRKILFNGLSAN------------------GVSYSDSKGKIHT 840
            LLNK    NLKV V+ATV +I+F+   +N                  GV YSDSKGK HT
Sbjct: 823  LLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSDSKGKSHT 882

Query: 841  AFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGAT 900
              +R KGEI LSAGAIGSP LLL SG+GP S+LSSL +P+V  QP VG+F++DNPR    
Sbjct: 883  VLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNIN 942

Query: 901  IVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKF 960
            +++PF    S+V+VVG + D+  ++  ++  P    P    + P++++ N  + I   K 
Sbjct: 943  LIIPFPTEPSAVQVVG-ITDHYFMETISANLPNSPTPLPFSMYPKSSTANLGMAIICEKL 1002

Query: 961  SEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQD 1015
                S G L L S+ D +    VRFNY+SHP DL++CV  VRK+G++L +++ME+ K  D
Sbjct: 1003 RHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMVD 1062

BLAST of Carg08367 vs. ExPASy TrEMBL
Match: A0A7J6GP35 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016302 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 1.1e-297
Identity = 540/1047 (51.58%), Postives = 695/1047 (66.38%), Query Frame = 0

Query: 1    MKFVQNGNTLPTREE-YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQ 60
            MK V +   +   EE YDYIIIGGGTAGCPLAATLS N+SVLVLERGS P A P+VL   
Sbjct: 100  MKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTANPNVLHLH 159

Query: 61   G-LANTLTDNDD-GLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQ 120
            G  AN + + ++  + P QRF SEDGVEN RGRVLGG SMIN GF+SR    FF   G++
Sbjct: 160  GFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGFFSKPGVK 219

Query: 121  WDMTAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIF 180
            W+M  VEKAY+W+EE++V RP+L  WQS+FR+ALLE GVGPDN +DL H +GT+  GS F
Sbjct: 220  WEMDGVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGTKISGSTF 279

Query: 181  DSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS-----------------AS 240
            D  GRRHGAVELLNK +  NL+V   ATV++IIFS     +                 A 
Sbjct: 280  DEVGRRHGAVELLNKGNLNNLKVVVHATVEKIIFSTKVSRNYHSGDDNDHDNNPPKPYAI 339

Query: 241  GVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHV 300
            GV+YSD KGK H   +   GE+IL+AGAIGSP LLL SG+GP S+LSSL +P+V    +V
Sbjct: 340  GVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVHSQPNV 399

Query: 301  GQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFP-PFGLLPPRS 360
            G  +ADNPR    +++P  T P+ VQVVG +  +  IE++S+ LP S+ P PF + P  S
Sbjct: 400  GDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPFSVYPKSS 459

Query: 361  TAVNLSLAVFAGKFSTVSSVGSLRL----DGRKNPIVRFNYLSHPDDVERCVEGVRKVGD 420
            TA +L + V   K     S GSL L    D R  P VRFNY SHP D+ +CV GVRK+G+
Sbjct: 460  TA-HLGMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVNGVRKIGE 519

Query: 421  LVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGK 480
            ++ T +MER K  D   ++ F + G  LP N SD   + +FCR +VTT WHYHGGC VGK
Sbjct: 520  MLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGK 579

Query: 481  VVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQR----------- 540
            VVD ++RV+G+  LRVVDGSTF +SPGTNP ATVMMLGRYVGLKMLQ+R           
Sbjct: 580  VVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEANVLALAT 639

Query: 541  ----LDVSYMKFVHNAGDLP-EKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPN 600
                 D  YMK V++A ++  E++ YDYIIIGGG AGCPLAATLS  + VL+LERGS P 
Sbjct: 640  SGSDHDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPT 699

Query: 601  KYPSVLNEQGLLNAFLAQDDGQ--NPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHR 660
              P+VL+  G L   + +++     P QRFTSEDGVEN+RGR+LGG +M+NAGF+SRG  
Sbjct: 700  SNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDE 759

Query: 661  QFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLI 720
             F+  +GV W+M+ VE AY+WVEE++V +P L  WQS+F+ ALLE GV P+NGF+L H +
Sbjct: 760  GFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVVPNNGFDLNHKV 819

Query: 721  GTKTGGSIFDGKGNRHGAVELLNKAEPKNLKVAVNATVRKILFNGLSANGVSYSDSKGKI 780
            GTK  GS FD  G RHG     + ++  + K                A GV YSDSKGK 
Sbjct: 820  GTKISGSTFDEVGRRHGNYHSGDDSDQNSPKP--------------YAIGVIYSDSKGKS 879

Query: 781  HTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFG 840
            HT  ++ KGE+ LSAGAIGSP LLL SG+GP S+LSSL +P+V  QP VG+F++DNPR  
Sbjct: 880  HTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNN 939

Query: 841  ATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVG 900
              +++PF    S+V+VVG + D+  ++  ++  P    P    + P++++ N  + I   
Sbjct: 940  INLIIPFPTEPSAVQVVG-ITDHYFMETISANLPNSPTPLPFSMYPKSSTANLGMAIICE 999

Query: 901  KFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKT 960
            K     S G L L S+ D +    VRFNY+SHP DL++CV  VRK+G++L +++ME+ K 
Sbjct: 1000 KLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKM 1059

Query: 961  QDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMK 1005
             D    + F + G  LP N  D+S++EE+CR +VTT WHYHGGC VGKVVDGD+RV G+ 
Sbjct: 1060 VDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVN 1119

BLAST of Carg08367 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 8.7e-146
Identity = 251/514 (48.83%), Postives = 346/514 (67.32%), Query Frame = 0

Query: 509  YMKFVHNAGDLPEKQEYDYIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQ 568
            +M+F+ NA D   +  YDYII+GGG AGCPLAATLS  F+VLLLERG  P   P+V++  
Sbjct: 38   FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 569  GLLNAFLAQDDGQNPFQRFTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWD 628
            G L      ++  +P Q F SE+GV N RGR+LGG + +NAGFYSR  +QFFE +G+ WD
Sbjct: 98   GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 629  MEMVENAYQWVEETVVSKPILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDG 688
            +  V  +Y+WVE  +V +P L  WQ+A + ALLE GV P NGF L H +GTK GGS FD 
Sbjct: 158  LSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDR 217

Query: 689  KGNRHGAVELLNKAEPKNLKVAVNATVRKILF--------NGLSANGVSYSDSKGKIHTA 748
             G RH + +LL  A   N++VAV ATV ++L         + +SA GV Y D  G+ H A
Sbjct: 218  TGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHA 277

Query: 749  FIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATI 808
             IR +GE+ LSAGA+GSP LL  SG+GP+S+LS+  +PV   QP+VG+F+ DNPR G +I
Sbjct: 278  LIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISI 337

Query: 809  VLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAPPTFSLLPPQATSINPSLVIFVGKFS 868
            V P  + +S ++VVG  +D   L+A ++  PF +P     +   A+ +   +   + K  
Sbjct: 338  VPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKIL 397

Query: 869  EVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDL 928
               S G LRL ++TD + N +VRFNY+S P DL RCV G RK+G++L+++ M+    ++ 
Sbjct: 398  GPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 457

Query: 929  EGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLR 988
             GN+ F+F+G PLP +  +D  + ++CR+TV+T WHYHGG +VGKVVD D +VIG+ +LR
Sbjct: 458  FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 517

Query: 989  VVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQER 1015
            +VDGSTF+ SPGTNP ATLMMLGRY+GLK+L+ER
Sbjct: 518  LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Carg08367 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 412.9 bits (1060), Expect = 1.0e-114
Identity = 236/551 (42.83%), Postives = 320/551 (58.08%), Query Frame = 0

Query: 3   FVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLA 62
           F+++    PT   YDYIIIGGGTAGCPLAATLS N SVL+LERG  P   P++       
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFG 92

Query: 63  NTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTA 122
             L+D  +  +P QRFVSEDGV N R RVLGGGS +N GFY+RA  ++ ++ G  WD   
Sbjct: 93  AALSDLSES-SPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGAL 152

Query: 123 VEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGR 182
             ++YQW+E  V  +P +  WQ+A R+ LLEAG+ P+NG+   H  GT+ GG+IFD  G 
Sbjct: 153 ANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGN 212

Query: 183 RHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLS---ASGVLYSDFKGKLHKATISKN 242
           RH A +LL  ADP+ + V   ATV RI+F ++ G +   A+GV+Y D  G+ H+A + + 
Sbjct: 213 RHTAADLLEYADPKGITVLLHATVHRILF-RTRGTTKPIANGVVYRDRTGQAHRAYLKEG 272

Query: 243 --GEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLP 302
              EIIL+AG +GSP LL+ SGVGP + L +  + VV+   HVGQ M DNP     +  P
Sbjct: 273 ALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSP 332

Query: 303 FLTPPTSVQVVGTLKRNIHIE----------------SLSSILPFSIFPPFGLLPPRSTA 362
                + ++VVG      ++E                S S+   +++F P   L   ++ 
Sbjct: 333 VPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSM 392

Query: 363 VNLSLA-VFAGKFSTVS-----SVGSLRLDGRK---NPIVRFNYLSHPDDVERCVEGVRK 422
             LS A  F G F         S G L L  R    NPIV FNY  HPDD++RCV G++ 
Sbjct: 393 TKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQT 452

Query: 423 VGDLVNTKSMERIKTGDLEGKKGFKFL------------------GAPLPENMSDYKLVG 482
           +  +V +K+  R K  D+     F++L                  GA LP +        
Sbjct: 453 IERVVQSKAFSRYKYADV----SFEYLLNLTASTPVNLRPPRSGPGASLPPSAE------ 512

Query: 483 DFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGR 506
           +FC+ TVTT WHYHGGC+VG+VVD +Y+VIGI +LRV+D ST    PGTNP ATVMMLGR
Sbjct: 513 EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGR 569

BLAST of Carg08367 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 411.8 bits (1057), Expect = 2.3e-114
Identity = 237/531 (44.63%), Postives = 312/531 (58.76%), Query Frame = 0

Query: 16  YDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLANTLTDNDDGLNPF 75
           YDYI+IGGGTAGCPLAATLS NFSVLVLERG  P    +V   +     L D     +  
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLAD-ISASSAS 122

Query: 76  QRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTAVEKAYQWIEETVV 135
           Q FVS DGV N R RVLGGGS IN GFYSRA   F K +G  WD   V+++Y W+E  +V
Sbjct: 123 QAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIV 182

Query: 136 SRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGRRHGAVELLNKADP 195
            +P+L+ WQ A R++LLE GV P NG+   H  GT+ GG+IFD  GRRH A ELL  A+P
Sbjct: 183 HQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANP 242

Query: 196 RNLRVATQATVKRIIFSQSNGL-SASGVLYSDFKGKLHKATIS--KNGEIILTAGAIGSP 255
           + LRV   ATV++I+F  S      +GV++ D KG  H+A +S  K  E+IL++GAIGSP
Sbjct: 243 QKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSP 302

Query: 256 HLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTP--PTSVQVVGT 315
            +L+ SG+GPK  L  LK+PVVL N HVG+ MADNP     I++P   P   + +Q VG 
Sbjct: 303 QMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTVGI 362

Query: 316 LKRNIHIESLSSI--LPFSIFPPFGLLP---------------PRSTAVNLSL------A 375
            K  +++E+ +     P SI   +G++                P +T   ++        
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 376 VFAGKF-----STVSSVGSLRLDGRK---NPIVRFNYLSHPDDVERCVEGVRKVGDLVNT 435
            F G F     +   S G L L       NP V FNY  HP D++RCVE +R V  +V +
Sbjct: 423 AFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTS 482

Query: 436 KSMERIKTGDLEGKKGFKFLGAPL-----PENMSDYKLVGDFCRKTVTTFWHYHGGCLVG 495
                    D +       L         P+ ++D K +  FC+ TV T WHYHGGCLVG
Sbjct: 483 NRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVG 542

Query: 496 KVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQRL 506
           KVV  N +V+G+ +LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L++RL
Sbjct: 543 KVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERL 588

BLAST of Carg08367 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 3.8e-109
Identity = 235/553 (42.50%), Postives = 308/553 (55.70%), Query Frame = 0

Query: 3   FVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLA 62
           F+++    P    +DYIIIGGGT+GC LAATLS N SVLVLERG  P   P+    +  A
Sbjct: 33  FMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFA 92

Query: 63  NTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTA 122
            TL+ N    +  Q F+SEDGV N R RVLGGGS++N GFY+RA  E+ K +  +W    
Sbjct: 93  TTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--EWKTDE 152

Query: 123 VEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGR 182
           VE AY+W+E+ V  +P +  WQ+AF++ LLEAG  P NG+   H  GT+ GG+IFD  G 
Sbjct: 153 VEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGH 212

Query: 183 RHGAVELLNKADPRNLRVATQATVKRIIFSQSN--GLSASGVLYSDFKGKLHKATISKN- 242
           RH A +LL  A+P N+ V   A+V +I+F+        A GV++ D  G LHKA + KN 
Sbjct: 213 RHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNS 272

Query: 243 -GEIILTAGAIGSPHLLLQSGVGPKSHLSSLKL-PVVLHNRHVGQSMADNPRFGAAIVLP 302
             E+IL+AGAIGSP LL+ SG+GP +HL++  + P+VL +  VGQ M DNP     I  P
Sbjct: 273 MNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSP 332

Query: 303 FLTPPTSVQVVGTLKRNIHIESLSSILPFS------------------------------ 362
                + +QVVG  K   +IE  S ++ FS                              
Sbjct: 333 TPVEVSLIQVVGITKFESYIEGASGVI-FSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPT 392

Query: 363 --------IFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRK---NPIVRFNY 422
                    F P   L   +T   L L   AG  S     G L L       NP VRFNY
Sbjct: 393 LSTQSITDFFNPINPLLNATTRAGLILQKIAGPISR----GHLELRNTNPDDNPSVRFNY 452

Query: 423 LSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVG-- 482
              P+D++ CVEG+  +  ++N+K+  + K  D     G   L   +P N+    +    
Sbjct: 453 YQEPEDLQICVEGINTIIKVINSKAFSKFKYPDAT-IHGLLDLMLSVPTNLRPRHITSMF 512

Query: 483 ---DFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMM 505
               FC  TV T WHYHGGC VG+VVD NYRV+GI  LRV+DGSTF  SPGTNP ATVMM
Sbjct: 513 NLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMM 572

BLAST of Carg08367 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 1.9e-108
Identity = 234/552 (42.39%), Postives = 307/552 (55.62%), Query Frame = 0

Query: 4   VQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLAN 63
           +++    P    +DYIIIGGGT+GC LAATLS N SVLVLERG  P   P+    +  A 
Sbjct: 1   MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60

Query: 64  TLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTAV 123
           TL+ N    +  Q F+SEDGV N R RVLGGGS++N GFY+RA  E+ K +  +W    V
Sbjct: 61  TLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--EWKTDEV 120

Query: 124 EKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGRR 183
           E AY+W+E+ V  +P +  WQ+AF++ LLEAG  P NG+   H  GT+ GG+IFD  G R
Sbjct: 121 EAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHR 180

Query: 184 HGAVELLNKADPRNLRVATQATVKRIIFSQSN--GLSASGVLYSDFKGKLHKATISKN-- 243
           H A +LL  A+P N+ V   A+V +I+F+        A GV++ D  G LHKA + KN  
Sbjct: 181 HTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSM 240

Query: 244 GEIILTAGAIGSPHLLLQSGVGPKSHLSSLKL-PVVLHNRHVGQSMADNPRFGAAIVLPF 303
            E+IL+AGAIGSP LL+ SG+GP +HL++  + P+VL +  VGQ M DNP     I  P 
Sbjct: 241 NEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPT 300

Query: 304 LTPPTSVQVVGTLKRNIHIESLSSILPFS------------------------------- 363
               + +QVVG  K   +IE  S ++ FS                               
Sbjct: 301 PVEVSLIQVVGITKFESYIEGASGVI-FSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTL 360

Query: 364 -------IFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRK---NPIVRFNYL 423
                   F P   L   +T   L L   AG  S     G L L       NP VRFNY 
Sbjct: 361 STQSITDFFNPINPLLNATTRAGLILQKIAGPISR----GHLELRNTNPDDNPSVRFNYY 420

Query: 424 SHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLGAPLPENMSDYKLVG--- 483
             P+D++ CVEG+  +  ++N+K+  + K  D     G   L   +P N+    +     
Sbjct: 421 QEPEDLQICVEGINTIIKVINSKAFSKFKYPDAT-IHGLLDLMLSVPTNLRPRHITSMFN 480

Query: 484 --DFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMML 505
              FC  TV T WHYHGGC VG+VVD NYRV+GI  LRV+DGSTF  SPGTNP ATVMML
Sbjct: 481 LRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMML 540