Carg08311 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08311
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Methionyl-tRNA synthetase
Location	Carg_Chr05: 3324469 .. 3324882 (-)
RNA-Seq Expression	Carg08311
Synteny	Carg08311

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACGCGGTTCTCCACCATAACAGGCCAAACCCCAAGGAAACCTCAAATTCCCTGAAACTTCCTTCTCTCTCTATAAAGTCTTCCAAGTCCACCACCTTACCAACCAGATTTATCACCCAAAACCCAAAAAAGAAATCAGGAAGCAAACAAACAATAACAGAAAGAAGCTTAGAAGCCAAAGAAAAGCAAGAACCTGAAGGAGGTGACAAGATGTGTTTGGTGTTCGTGTGCGATGAGGATCAGAGGGTGCTGTCCAGGCAACCAGCTCCAGGCGCTTGCCCCTTCTGCGGAGGCATGATCCAGGCCACCGACGTTGAAAGCCAATGGAGGTTCTGCTTCCTCCCCCTTTACTGGAAAACCAAACGCAAGTTCTATTGCACCATGTGCACCAGACAACTCGTTATACAGTAG

mRNA sequence

ATGCACGCGGTTCTCCACCATAACAGGCCAAACCCCAAGGAAACCTCAAATTCCCTGAAACTTCCTTCTCTCTCTATAAAGTCTTCCAAGTCCACCACCTTACCAACCAGATTTATCACCCAAAACCCAAAAAAGAAATCAGGAAGCAAACAAACAATAACAGAAAGAAGCTTAGAAGCCAAAGAAAAGCAAGAACCTGAAGGAGGTGACAAGATGTGTTTGGTGTTCGTGTGCGATGAGGATCAGAGGGTGCTGTCCAGGCAACCAGCTCCAGGCGCTTGCCCCTTCTGCGGAGGCATGATCCAGGCCACCGACGTTGAAAGCCAATGGAGGTTCTGCTTCCTCCCCCTTTACTGGAAAACCAAACGCAAGTTCTATTGCACCATGTGCACCAGACAACTCGTTATACAGTAG

Coding sequence (CDS)

ATGCACGCGGTTCTCCACCATAACAGGCCAAACCCCAAGGAAACCTCAAATTCCCTGAAACTTCCTTCTCTCTCTATAAAGTCTTCCAAGTCCACCACCTTACCAACCAGATTTATCACCCAAAACCCAAAAAAGAAATCAGGAAGCAAACAAACAATAACAGAAAGAAGCTTAGAAGCCAAAGAAAAGCAAGAACCTGAAGGAGGTGACAAGATGTGTTTGGTGTTCGTGTGCGATGAGGATCAGAGGGTGCTGTCCAGGCAACCAGCTCCAGGCGCTTGCCCCTTCTGCGGAGGCATGATCCAGGCCACCGACGTTGAAAGCCAATGGAGGTTCTGCTTCCTCCCCCTTTACTGGAAAACCAAACGCAAGTTCTATTGCACCATGTGCACCAGACAACTCGTTATACAGTAG

Protein sequence

MHAVLHHNRPNPKETSNSLKLPSLSIKSSKSTTLPTRFITQNPKKKSGSKQTITERSLEAKEKQEPEGGDKMCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCTRQLVIQ

Homology

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BLAST of Carg08311 vs. NCBI nr
Match: KAG7029748.1 (hypothetical protein SDJN02_08090, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.7 bits (722), Expect = 1.7e-72
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MHAVLHHNRPNPKETSNSLKLPSLSIKSSKSTTLPTRFITQNPKKKSGSKQTITERSLEA 60
           MHAVLHHNRPNPKETSNSLKLPSLSIKSSKSTTLPTRFITQNPKKKSGSKQTITERSLEA
Sbjct: 1   MHAVLHHNRPNPKETSNSLKLPSLSIKSSKSTTLPTRFITQNPKKKSGSKQTITERSLEA 60

Query: 61  KEKQEPEGGDKMCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWK 120
           KEKQEPEGGDKMCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWK
Sbjct: 61  KEKQEPEGGDKMCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWK 120

Query: 121 TKRKFYCTMCTRQLVIQ 138
           TKRKFYCTMCTRQLVIQ
Sbjct: 121 TKRKFYCTMCTRQLVIQ 137

[Close]

BLAST of Carg08311 vs. NCBI nr
Match: XP_022962063.1 (uncharacterized protein LOC111462635 [Cucurbita moschata] >XP_022996793.1 uncharacterized protein LOC111491925 [Cucurbita maxima] >XP_023546190.1 uncharacterized protein LOC111805366 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 151.4 bits (381), Expect = 5.8e-33
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLVIQ
Sbjct: 61  RQLVIQ 66

[Close]

BLAST of Carg08311 vs. NCBI nr
Match: XP_038884125.1 (uncharacterized protein LOC120075047 [Benincasa hispida])

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-32
Identity = 65/66 (98.48%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLV+Q
Sbjct: 61  RQLVMQ 66

[Close]

BLAST of Carg08311 vs. NCBI nr
Match: XP_008445081.1 (PREDICTED: uncharacterized protein LOC103488227 [Cucumis melo])

HSP 1 Score: 149.1 bits (375), Expect = 2.9e-32
Identity = 64/66 (96.97%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCF+PLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFVPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLV+Q
Sbjct: 61  RQLVMQ 66

[Close]

BLAST of Carg08311 vs. NCBI nr
Match: XP_004138769.1 (uncharacterized protein LOC101206986 [Cucumis sativus])

HSP 1 Score: 147.5 bits (371), Expect = 8.3e-32
Identity = 63/66 (95.45%), Postives = 65/66 (98.48%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPG CPFCGGMIQATDVESQWRFCF+PLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGVCPFCGGMIQATDVESQWRFCFVPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLV+Q
Sbjct: 61  RQLVMQ 66

[Close]

BLAST of Carg08311 vs. ExPASy TrEMBL
Match: A0A6J1KC13 (uncharacterized protein LOC111491925 OS=Cucurbita maxima OX=3661 GN=LOC111491925 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-33
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLVIQ
Sbjct: 61  RQLVIQ 66

[Close]

BLAST of Carg08311 vs. ExPASy TrEMBL
Match: A0A6J1HDM3 (uncharacterized protein LOC111462635 OS=Cucurbita moschata OX=3662 GN=LOC111462635 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-33
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLVIQ
Sbjct: 61  RQLVIQ 66

[Close]

BLAST of Carg08311 vs. ExPASy TrEMBL
Match: A0A1S3BCK8 (uncharacterized protein LOC103488227 OS=Cucumis melo OX=3656 GN=LOC103488227 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.4e-32
Identity = 64/66 (96.97%), Postives = 66/66 (100.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCF+PLYWKTKRKFYCTMCT
Sbjct: 1   MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFVPLYWKTKRKFYCTMCT 60

Query: 132 RQLVIQ 138
           RQLV+Q
Sbjct: 61  RQLVMQ 66

[Close]

BLAST of Carg08311 vs. ExPASy TrEMBL
Match: A0A6P3Z2S4 (uncharacterized protein LOC107405842 OS=Ziziphus jujuba OX=326968 GN=LOC107405842 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.9e-30
Identity = 57/66 (86.36%), Postives = 65/66 (98.48%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDED+RV+SRQPAPGACP+CGGM+QA DVESQWRFCFLPLYWKTKRKFYC++CT
Sbjct: 1   MCLVFVCDEDERVVSRQPAPGACPYCGGMVQAMDVESQWRFCFLPLYWKTKRKFYCSLCT 60

Query: 132 RQLVIQ 138
           R+LV+Q
Sbjct: 61  RRLVVQ 66

[Close]

BLAST of Carg08311 vs. ExPASy TrEMBL
Match: A0A6J1BVT7 (uncharacterized protein LOC111005173 OS=Momordica charantia OX=3673 GN=LOC111005173 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 5.5e-29
Identity = 55/66 (83.33%), Postives = 64/66 (96.97%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCDE+QRVLSRQPAPGACPFCGGM+QATD+++QW FCFLPLYWKTKRK YC++CT
Sbjct: 1   MCLVFVCDENQRVLSRQPAPGACPFCGGMVQATDIQTQWSFCFLPLYWKTKRKLYCSICT 60

Query: 132 RQLVIQ 138
           R+LV+Q
Sbjct: 61  RELVVQ 66

[Close]

BLAST of Carg08311 vs. TAIR 10
Match: AT5G10695.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G57123.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 101.3 bits (251), Expect = 6.4e-22
Identity = 39/65 (60.00%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCD+D+RV+ R  APGACP+CGG +Q  DV SQWRFCF+PL  K+KR+  C+ C 
Sbjct: 1   MCLVFVCDQDERVIGRYAAPGACPYCGGAVQVVDVNSQWRFCFVPLSNKSKRRHLCSTCG 60

Query: 132 RQLVI 137
           ++L++
Sbjct: 61  KRLIV 65

[Close]

BLAST of Carg08311 vs. TAIR 10
Match: AT5G57123.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29905.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 94.4 bits (233), Expect = 7.8e-20
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLV +CDE++  L RQ APG+CP+CGG +Q  DVE +W FCF+PL +K KRK+ C+ C 
Sbjct: 1   MCLVCLCDEEETELGRQQAPGSCPYCGGKVQMLDVERKWMFCFVPLCFKIKRKYSCSSCD 60

Query: 132 RQLVI 137
           R+LV+
Sbjct: 61  RRLVL 65

[Close]

BLAST of Carg08311 vs. TAIR 10
Match: AT4G29905.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G57123.1); Has 73 Blast hits to 73 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 73; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 1.2e-17
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCL  V  E++  L RQ APG CP+CGG + A D+E++W FCFLPL +K KRK+ C+ C 
Sbjct: 1   MCLEVVYHEEKTELGRQQAPGMCPYCGGKVSAVDIETKWLFCFLPLCFKVKRKYSCSSCE 60

Query: 132 RQLVI 137
           R LV+
Sbjct: 61  RHLVL 65

[Close]

BLAST of Carg08311 vs. TAIR 10
Match: AT1G52855.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G15534.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 60.1 bits (144), Expect = 1.6e-09
Identity = 21/59 (35.59%), Postives = 35/59 (59.32%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMC 131
           +C  F+ D+ ++V   +PA G C  CG      D+++  RFCF+P+YW++ R   C+ C
Sbjct: 2   VCFCFLVDQTRKVKRSKPAAGTCSRCGHCASIADMKTSTRFCFIPIYWRSWRAVVCSFC 60

[Close]

BLAST of Carg08311 vs. TAIR 10
Match: AT5G10695.2 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 12 growth stages. )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 23/65 (35.38%), Postives = 34/65 (52.31%), Query Frame = 0


Query: 72  MCLVFVCDEDQRVLSRQPAPGACPFCGGMIQATDVESQWRFCFLPLYWKTKRKFYCTMCT 131
           MCLVFVCD+D+RV+                         RFCF+PL  K+KR+  C+ C 
Sbjct: 1   MCLVFVCDQDERVIG------------------------RFCFVPLSNKSKRRHLCSTCG 41

Query: 132 RQLVI 137
           ++L++
Sbjct: 61  KRLIV 41

The following BLAST results are available for this feature:

BLAST of Carg08311 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029748.1	1.7e-72	100.00	hypothetical protein SDJN02_08090, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022962063.1	5.8e-33	100.00	uncharacterized protein LOC111462635 [Cucurbita moschata] >XP_022996793.1 unchar...	[more]
XP_038884125.1	1.3e-32	98.48	uncharacterized protein LOC120075047 [Benincasa hispida]	[more]
XP_008445081.1	2.9e-32	96.97	PREDICTED: uncharacterized protein LOC103488227 [Cucumis melo]	[more]
XP_004138769.1	8.3e-32	95.45	uncharacterized protein LOC101206986 [Cucumis sativus]	[more]

BLAST of Carg08311 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg08311 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KC13	2.8e-33	100.00	uncharacterized protein LOC111491925 OS=Cucurbita maxima OX=3661 GN=LOC111491925...	[more]
A0A6J1HDM3	2.8e-33	100.00	uncharacterized protein LOC111462635 OS=Cucurbita moschata OX=3662 GN=LOC1114626...	[more]
A0A1S3BCK8	1.4e-32	96.97	uncharacterized protein LOC103488227 OS=Cucumis melo OX=3656 GN=LOC103488227 PE=...	[more]
A0A6P3Z2S4	4.9e-30	86.36	uncharacterized protein LOC107405842 OS=Ziziphus jujuba OX=326968 GN=LOC10740584...	[more]
A0A6J1BVT7	5.5e-29	83.33	uncharacterized protein LOC111005173 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]

BLAST of Carg08311 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10695.1	6.4e-22	60.00	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G57123.1	7.8e-20	56.92	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G29905.1	1.2e-17	53.85	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G52855.1	1.6e-09	35.59	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G10695.2	1.4e-05	35.38	unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_c...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..67
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..67
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..48
None	No IPR available	PANTHER	PTHR33320:SF20	METHIONYL-TRNA SYNTHETASE	coord: 72..137
None	No IPR available	PANTHER	PTHR33320	METHIONYL-TRNA SYNTHETASE	coord: 72..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08311-RA	Carg08311-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity