Carg08285 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08285
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ubiquitin-conjugating enzyme E2
Location	Carg_Chr05: 3169824 .. 3173069 (-)
RNA-Seq Expression	Carg08285
Synteny	Carg08285

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGTTAGCCACTATACTTAGTTATGTATATCATGAGTCCACACGAAGGGATCCGACTACGAGTATGTTGAGGATTGTTGGGAGTGAGTACCACGTTGGCCAATTTAGGGAACGATCATGTGTTTATAAGCAAAATACCTCTCTATTGGTATGAGGCATTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCATAGTAGACAATATCATACCATTGTGGAGAGTCATGATTCCTAACATGGCATTAGAGCCCTGCCGTAAACCTAGCTATGACAATAGGATCCTCAAATATTGAACAAAGAATTGTGAGCCTTGAAGGTGTAGTCAAAAGTGACTAAAGTGTTGAACAAAGGGTGTACTTTGTTCGAGGGCTCCAGAGAAAGGAGTCGAGTCTCGATTAAGGGGAGACTTATAGTGTATTTTGTTCGAGTTGAGGATCGTTGGGAGTGTGTCTCACATTGGCTAATTTAGAGAATGATCATGGATTTATAAGTAGAGTAATATCTCTCCATTGGTATGAAGCCTTTTGGGGAAGTCCAAAGCAAAGCTATGAGAGCTTATGCTCAAAGTGGACAATATCATATCATTGTGGAGAGTTGTGATTCCTAGTAGAGGTTCAGTCAAGTATCATGTCTGAGCCTTTGATTTTGTGTAGGCGTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAGTTGGTTTGTGACGATTCCAATATTTTTAAATGGATAGCGCTAATCAAGGTAACCCTCTCAATGATCCAGATGAAATATGTGGTTTCAGAGTTTTTCTTTTCTTTTTTTTTTTTTTTTTGCTTACCATGATTGATATGCATTTTTATTTAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGGTAGAAGTTGCTCTGGTCCTTTTTCTAGATCCTATGCTGACTATGAGCCTTTAGAAAGAAAGAAGAATACACCAAAATGTAGGTGCTGTACCGTTGTTGTAAGTGAATGTAATGAAAAGATCGATGCGAGCCGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGATCTCAAAATAATAGGGGTTAAGGTGGGCCGGTGACACGTAGGGGATAAGCTTGATCATGGGGGAGGAAAGGGAAACACTAGGGGAGGGGGTACCGTTGTCCAAGGGAGAGTAGTGAGCAGCAGTATTTAGGTCGTTTTTTAATTAGTTAACAAATACCGTTGAGCATCTGTGCATGGAGGGGAATATGGTGGAGAATATATCAGAGTGAGATCCCAGCGTCAGCGAGGATGTTGGGCCCTAAAGGGGATGGATTGCGAGATCCCACATCGGTTGGAGAGGGCAACAAAACATTCTTTATAAGGGTGTGGAAATCTCTCCCTAGCTGACGCGTTTTAAAACCTCGAGGGGAAGTCTCGAAGGAAAAGCGCAAAGAGGACAATATCTGCTAGTTGTGAAGGTACACGGGAGTTGCAATGATGCTAGCGGTGGAGGTACCACGGGAGAAGAGTTTACGACAAGCAACCGGGTAAAAAGGAAGTGAACTCAATAGAAAGAAGCGACTGGGATTAGGAGTGGGAAGCTAGATATTTCGTGGACTGCTTTGTTCATATGAGAGGAGTTCTTGTTGCAGAGCAATTCAGTGCATTAGTGAGTTGCAGTCTAAAGGGAGAAGGAGAGGTTCGGTATGATTTTGATGGGTGGCTTGGAGCCCGAAAGAGGGTGATCCGGTCACGGGACGAGTAGAGAACCCGAATATTCCTTATTCCAACTTTTGCAGAAGGCCATACTCAGTGAAAATTCATAGCTCTCCGGCCACTGGCATATGAAGTGCTTGTGTAACGCCATTGATTGAAATAGTATAAGTTGCATTCTTTGCTATGAACTATATTGATGAATAGTGATCATTCAAGTTCGCCGACCCCTTTTGTGATTGTAGTCTTAGTTTATAGTGAACCTTATTTTTAATACACGAATCGAACCACACTTATACTTACCGATGTACGTCGTTCCTGCAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGTGAGTTTTCATGGATTCCTTATGATGAATTTTCCTGATTTATATGTTCATTTATTTGAATCTTTGTGATGGCGTGATAATGTAGCAAAGTAGTTAATTGATCTGAAAACTGCTTCTGGTTCGCGATTGAATTAGTTCATCATTCCTAAACCATTCTTTTGGAGAATGATTTGCATAATAGTTAAGAATAGATGCAAAAATTATGGGAAGAACACTGAGCTAGTCGCTTGAATCTATAGGCAACCTCTCCTCGATCAAACAAACTCTGAATTTCATAAGAGAATCAAGATCGTAGTTGCATCAGTTTGGCTCGGGTCTAGGCCGATACTGATGTAAACTAGCTTATCGATTTATGAATATAAAAACCCAATAAGACTAAACCAGTTGAGTTCAGTTGTATATTTTTAAATTGAATCGATTGATAATGCTCGAGATATTGGGGAATTTCACTCCTTAGGAAGGCGTTGTGCATTGTGAGCCAGCTTCAAGGAGGCAACTAGAATTCAATTTTTAATGAATACGGCTCCGTTTGATAACTATTTAGTTTTTAGTTTCTGAAAAGTGTGCTTATTTTCTCGTAATTTCTTTAGAATAATTTATCTTTCTGAAGGCAATATTTTGGTGAAAGTTGTGTTTATAAGCTAAATTTCCAAAATAAAAAATCGAAAAGGCTCGAACTTAGGTCTGGGGTGATTCAACTTAAGACCTTGGAGAGAATCTCCGAACATTCTAAGTACAACGGTATCATTTGGAGGTGAAATAATTGAAGCGTGTTGCTCGGTTTCATAAACTTTTTTTGGGATCAAACCCCGGTAACACATATAAAGCAATTCGAGTGATCTTCAATTCTTCTGACCGATTGTTTGGGTGTTTACAGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

mRNA sequence

ATGCAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

Coding sequence (CDS)

ATGCAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

Protein sequence

MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG

Homology

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BLAST of Carg08285 vs. NCBI nr
Match: KAG7029722.1 (Protein PEROXIN-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 248.8 bits (634), Expect = 2.3e-62
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 117

[Close]

BLAST of Carg08285 vs. NCBI nr
Match: XP_022962320.1 (protein PEROXIN-4-like [Cucurbita moschata] >XP_022996597.1 protein PEROXIN-4-like [Cucurbita maxima] >XP_023545582.1 protein PEROXIN-4-like [Cucurbita pepo subsp. pepo] >KAG6598784.1 Protein PEROXIN-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 245.7 bits (626), Expect = 1.9e-61
Identity = 115/117 (98.29%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. NCBI nr
Match: XP_022857422.1 (protein PEROXIN-4-like [Olea europaea var. sylvestris])

HSP 1 Score: 245.4 bits (625), Expect = 2.5e-61
Identity = 114/117 (97.44%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. NCBI nr
Match: KAF1871641.1 (hypothetical protein Lal_00020435 [Lupinus albus])

HSP 1 Score: 244.2 bits (622), Expect = 5.6e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 708 IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 767

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 768 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 824

[Close]

BLAST of Carg08285 vs. NCBI nr
Match: KYP65178.1 (putative ubiquitin carrier protein E2 21 [Cajanus cajan])

HSP 1 Score: 244.2 bits (622), Expect = 5.6e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 251 IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 310

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 311 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 367

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BLAST of Carg08285 vs. ExPASy Swiss-Prot
Match: Q8LGF7 (Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 2.6e-61
Identity = 109/117 (93.16%), Postives = 114/117 (97.44%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETP+EGGVFQLA SVPE YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+MYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. ExPASy Swiss-Prot
Match: Q86IZ3 (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex4 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.4e-43
Identity = 75/110 (68.18%), Postives = 97/110 (88.18%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GP +TP+E G F+L +SVP  YPLQPP ++F+TKIFHPN+HFKTGEICLD+LK +WS 
Sbjct: 37  IKGPPDTPYENGKFELDISVPTNYPLQPPTIKFVTKIFHPNIHFKTGEICLDLLKTSWSA 96

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRL 111
            +TLQSVCR+IIAL++ PE DSPLNCD+GNL+R+GDV+G+ S+ARMYTRL
Sbjct: 97  IYTLQSVCRSIIALLSLPEADSPLNCDAGNLIRNGDVKGHDSLARMYTRL 146

[Close]

BLAST of Carg08285 vs. ExPASy Swiss-Prot
Match: Q9P6I1 (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc16 PE=3 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 3.1e-30
Identity = 60/115 (52.17%), Postives = 80/115 (69.57%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GP+ETP+EGG + L + V E YP+ PP V F TKI HPN+ +  GE+C+DILK  WSP
Sbjct: 41  IEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPK 116
           AW+LQS C AII+L+++ +  SPLN D+  LLR+GD   Y S+ R  T L A  K
Sbjct: 101 AWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRCTTYLYAKGK 155

[Close]

BLAST of Carg08285 vs. ExPASy Swiss-Prot
Match: O60015 (Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 5.5e-27
Identity = 56/129 (43.41%), Postives = 78/129 (60.47%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLT----------------------KIF 60
           ++GP ++PFE  +++L + +P  YPL PP+++F+                       K+ 
Sbjct: 48  IRGPDQSPFEDALWKLEIDIPTNYPLDPPKIKFVVFGEEKIRQLQRKTSSGARKVCYKMP 107

Query: 61  HPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVR 108
           HPNV+FKTGEICLDIL+  WSPAWTLQS   AI+ L+A+PEP SPLN D  NLL+  D  
Sbjct: 108 HPNVNFKTGEICLDILQQKWSPAWTLQSALVAIVVLLANPEPLSPLNIDMANLLKCDDTT 167

[Close]

BLAST of Carg08285 vs. ExPASy Swiss-Prot
Match: Q9EQX9 (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 5.1e-25
Identity = 56/111 (50.45%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP ++PFEGG F+L L +PE+YP+  P+VRF+TKI+HPNV  K G ICLDILK+ WSPA 
Sbjct: 41  GPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVD-KLGRICLDILKDKWSPAL 100

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAM 114
            +++V  +I AL++ P PD PL  D     +S + +  ++ AR +TRL AM
Sbjct: 101 QIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET-ARAWTRLYAM 149

[Close]

BLAST of Carg08285 vs. ExPASy TrEMBL
Match: A0A6J1K2D7 (protein PEROXIN-4-like OS=Cucurbita maxima OX=3661 GN=LOC111491789 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 9.3e-62
Identity = 115/117 (98.29%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. ExPASy TrEMBL
Match: A0A6J1HEQ0 (protein PEROXIN-4-like OS=Cucurbita moschata OX=3662 GN=LOC111462805 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 9.3e-62
Identity = 115/117 (98.29%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. ExPASy TrEMBL
Match: A0A6J1KTV6 (protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.7e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. ExPASy TrEMBL
Match: A0A6P4BYD2 (protein PEROXIN-4 OS=Arachis duranensis OX=130453 GN=LOC107470764 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.7e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. ExPASy TrEMBL
Match: A0A0B2QDV8 (Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=D0Y65_014097 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.7e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. TAIR 10
Match: AT5G25760.1 (peroxin4 )

HSP 1 Score: 235.7 bits (600), Expect = 1.9e-62
Identity = 109/117 (93.16%), Postives = 114/117 (97.44%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETP+EGGVFQLA SVPE YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+MYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. TAIR 10
Match: AT5G25760.2 (peroxin4 )

HSP 1 Score: 235.7 bits (600), Expect = 1.9e-62
Identity = 109/117 (93.16%), Postives = 114/117 (97.44%), Query Frame = 0


Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETP+EGGVFQLA SVPE YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+MYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of Carg08285 vs. TAIR 10
Match: AT1G16890.1 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 110.9 bits (276), Expect = 6.9e-25
Identity = 53/110 (48.18%), Postives = 79/110 (71.82%), Query Frame = 0


Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP+++P+EGGVF+L L +PE+YP+  P+VRFLTKI+HPN+  K G ICLDILK+ WSPA 
Sbjct: 10  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-KLGRICLDILKDKWSPAL 69

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA 113
            +++V  +I AL++ P PD PL+ +     +S +    ++ A+ +TRL A
Sbjct: 70  QIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKEWTRLYA 117

[Close]

BLAST of Carg08285 vs. TAIR 10
Match: AT1G16890.2 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 110.9 bits (276), Expect = 6.9e-25
Identity = 53/110 (48.18%), Postives = 79/110 (71.82%), Query Frame = 0


Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP+++P+EGGVF+L L +PE+YP+  P+VRFLTKI+HPN+  K G ICLDILK+ WSPA 
Sbjct: 43  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-KLGRICLDILKDKWSPAL 102

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA 113
            +++V  +I AL++ P PD PL+ +     +S +    ++ A+ +TRL A
Sbjct: 103 QIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKEWTRLYA 150

[Close]

BLAST of Carg08285 vs. TAIR 10
Match: AT1G16890.3 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 110.9 bits (276), Expect = 6.9e-25
Identity = 53/110 (48.18%), Postives = 79/110 (71.82%), Query Frame = 0


Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP+++P+EGGVF+L L +PE+YP+  P+VRFLTKI+HPN+  K G ICLDILK+ WSPA 
Sbjct: 52  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-KLGRICLDILKDKWSPAL 111

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA 113
            +++V  +I AL++ P PD PL+ +     +S +    ++ A+ +TRL A
Sbjct: 112 QIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKEWTRLYA 159

The following BLAST results are available for this feature:

BLAST of Carg08285 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029722.1	2.3e-62	100.00	Protein PEROXIN-4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022962320.1	1.9e-61	98.29	protein PEROXIN-4-like [Cucurbita moschata] >XP_022996597.1 protein PEROXIN-4-li...	[more]
XP_022857422.1	2.5e-61	97.44	protein PEROXIN-4-like [Olea europaea var. sylvestris]	[more]
KAF1871641.1	5.6e-61	97.44	hypothetical protein Lal_00020435 [Lupinus albus]	[more]
KYP65178.1	5.6e-61	97.44	putative ubiquitin carrier protein E2 21 [Cajanus cajan]	[more]

BLAST of Carg08285 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGF7	2.6e-61	93.16	Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1	[more]
Q86IZ3	1.4e-43	68.18	Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex...	[more]
Q9P6I1	3.1e-30	52.17	Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]
O60015	5.5e-27	43.41	Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 ...	[more]
Q9EQX9	5.1e-25	50.45	Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV...	[more]

BLAST of Carg08285 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K2D7	9.3e-62	98.29	protein PEROXIN-4-like OS=Cucurbita maxima OX=3661 GN=LOC111491789 PE=3 SV=1	[more]
A0A6J1HEQ0	9.3e-62	98.29	protein PEROXIN-4-like OS=Cucurbita moschata OX=3662 GN=LOC111462805 PE=3 SV=1	[more]
A0A6J1KTV6	2.7e-61	97.44	protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1	[more]
A0A6P4BYD2	2.7e-61	97.44	protein PEROXIN-4 OS=Arachis duranensis OX=130453 GN=LOC107470764 PE=3 SV=1	[more]
A0A0B2QDV8	2.7e-61	97.44	Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=D0Y65_014097 PE=3 SV=1	[more]

BLAST of Carg08285 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25760.1	1.9e-62	93.16	peroxin4	[more]
AT5G25760.2	1.9e-62	93.16	peroxin4	[more]
AT1G16890.1	6.9e-25	48.18	ubiquitin-conjugating enzyme 36	[more]
AT1G16890.2	6.9e-25	48.18	ubiquitin-conjugating enzyme 36	[more]
AT1G16890.3	6.9e-25	48.18	ubiquitin-conjugating enzyme 36	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 1..113 e-value: 3.8E-29 score: 112.8
None	No IPR available	PANTHER	PTHR24068:SF383	BNAA09G04490D PROTEIN	coord: 2..117
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 2..117
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 1..107 e-value: 9.6E-39 score: 132.2
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 1..113 score: 42.282799
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 3..108 e-value: 3.77269E-43 score: 136.178
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 1..112 e-value: 1.7E-42 score: 146.9
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 3..113
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 38..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08285-RA	Carg08285-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0016020	membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0035091	phosphatidylinositol binding
molecular_function	GO:0043130	ubiquitin binding
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity