Carg08281 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08281
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiondehydration-responsive element-binding protein 3-like
LocationCarg_Chr05: 3137565 .. 3138206 (-)
RNA-Seq ExpressionCarg08281
SyntenyCarg08281
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAGAAACGAAAACGGCACAACAACGTGTTCGTCTCCGGAGCCGAACAAGAAGCCGAAACGCATCAGAGACACAGAGGACTCGAAGCACCCGCTTTACAGAGGCGTCCGAATGAGAAATTGGGGGAAATGGGTGTCCGAAATTCGTGAGCCAAGAAAGAAATCCCGCATTTGGTTAGGCACTTTCCCCAGCCCTGAAATGGCTGCACGAGCCCACGATGTAGCCGCCTTAAGCATCAAAGGAAAAGCCGCAATCCTTAATTTCCCCGACCTGGTACACCTCCTACCTCGCCCTGTTTCACTCGCCCCTCGCGACGTTCAGGCCGCCGCCGCTAAAGCCGCTCATATGCAACATTTCTCCTCTACTCATACTAATAATAACAATTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCGATGCCACCGATGAGCTCACGGAAATCGTCCAGCTACCGAGTCTCGGAACGAGTTACGACGGCGGCGACGGCGGATTCGGTGGTGAGTTTGTGTTTTTTGACTCGGGGCAGGAATTGGAATCTGGGTGTTGGATGTACACGCCGCCATGGGTGCAGAGCTTGGAGGATTGTGACAATGATGATTATGGTAAGTTCGAAGGATTGCTGTGGGATTATTGA

mRNA sequence

ATGAGCAGAAACGAAAACGGCACAACAACGTGTTCGTCTCCGGAGCCGAACAAGAAGCCGAAACGCATCAGAGACACAGAGGACTCGAAGCACCCGCTTTACAGAGGCGTCCGAATGAGAAATTGGGGGAAATGGGTGTCCGAAATTCGTGAGCCAAGAAAGAAATCCCGCATTTGGTTAGGCACTTTCCCCAGCCCTGAAATGGCTGCACGAGCCCACGATGTAGCCGCCTTAAGCATCAAAGGAAAAGCCGCAATCCTTAATTTCCCCGACCTGGTACACCTCCTACCTCGCCCTGTTTCACTCGCCCCTCGCGACGTTCAGGCCGCCGCCGCTAAAGCCGCTCATATGCAACATTTCTCCTCTACTCATACTAATAATAACAATTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCGATGCCACCGATGAGCTCACGGAAATCGTCCAGCTACCGAGTCTCGGAACGAGTTACGACGGCGGCGACGGCGGATTCGGTGGTGAGTTTGTGTTTTTTGACTCGGGGCAGGAATTGGAATCTGGGTGTTGGATGTACACGCCGCCATGGGTGCAGAGCTTGGAGGATTGTGACAATGATGATTATGGTAAGTTCGAAGGATTGCTGTGGGATTATTGA

Coding sequence (CDS)

ATGAGCAGAAACGAAAACGGCACAACAACGTGTTCGTCTCCGGAGCCGAACAAGAAGCCGAAACGCATCAGAGACACAGAGGACTCGAAGCACCCGCTTTACAGAGGCGTCCGAATGAGAAATTGGGGGAAATGGGTGTCCGAAATTCGTGAGCCAAGAAAGAAATCCCGCATTTGGTTAGGCACTTTCCCCAGCCCTGAAATGGCTGCACGAGCCCACGATGTAGCCGCCTTAAGCATCAAAGGAAAAGCCGCAATCCTTAATTTCCCCGACCTGGTACACCTCCTACCTCGCCCTGTTTCACTCGCCCCTCGCGACGTTCAGGCCGCCGCCGCTAAAGCCGCTCATATGCAACATTTCTCCTCTACTCATACTAATAATAACAATTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCGATGCCACCGATGAGCTCACGGAAATCGTCCAGCTACCGAGTCTCGGAACGAGTTACGACGGCGGCGACGGCGGATTCGGTGGTGAGTTTGTGTTTTTTGACTCGGGGCAGGAATTGGAATCTGGGTGTTGGATGTACACGCCGCCATGGGTGCAGAGCTTGGAGGATTGTGACAATGATGATTATGGTAAGTTCGAAGGATTGCTGTGGGATTATTGA

Protein sequence

MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHFSSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Homology
BLAST of Carg08281 vs. NCBI nr
Match: KAG7029718.1 (Dehydration-responsive element-binding protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 428.7 bits (1101), Expect = 2.9e-116
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL
Sbjct: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 213

BLAST of Carg08281 vs. NCBI nr
Match: KAG6598779.1 (Dehydration-responsive element-binding protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 422.9 bits (1086), Expect = 1.6e-114
Identity = 213/217 (98.16%), Postives = 213/217 (98.16%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120

Query: 121 SSTHTNNNN----SSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS 180
           SSTHTNNNN    SSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS
Sbjct: 121 SSTHTNNNNSSSSSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS 180

Query: 181 GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 217

BLAST of Carg08281 vs. NCBI nr
Match: XP_022962316.1 (dehydration-responsive element-binding protein 3-like [Cucurbita moschata])

HSP 1 Score: 410.6 bits (1054), Expect = 8.2e-111
Identity = 205/213 (96.24%), Postives = 205/213 (96.24%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN TTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNENATTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAA M HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAARMHHF 120

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SSTHTNNNN     SSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL
Sbjct: 121 SSTHTNNNN-----SSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 208

BLAST of Carg08281 vs. NCBI nr
Match: XP_023546365.1 (ethylene-responsive transcription factor TINY-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392.9 bits (1008), Expect = 1.8e-105
Identity = 199/213 (93.43%), Postives = 200/213 (93.90%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MS NEN TTTCSSPEPNKKPKRIRDTEDS HPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 76  MSINENATTTCSSPEPNKKPKRIRDTEDSNHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 135

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHM HF
Sbjct: 136 GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMHHF 195

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SSTHTNNNN    SSSSSSSSDATDELTEIVQLP+LGTSY   DGGFGGEFVFFDSGQEL
Sbjct: 196 SSTHTNNNN----SSSSSSSSDATDELTEIVQLPTLGTSY---DGGFGGEFVFFDSGQEL 255

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMY PPWVQSL DCDNDDYGKFEGLLWDY
Sbjct: 256 ESGCWMYAPPWVQSLGDCDNDDYGKFEGLLWDY 281

BLAST of Carg08281 vs. NCBI nr
Match: XP_022996590.1 (dehydration-responsive element-binding protein 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 384.8 bits (987), Expect = 4.8e-103
Identity = 200/214 (93.46%), Postives = 202/214 (94.39%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN  TTCSSPEPNKK KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNEN-ATTCSSPEPNKKLKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAA KAAHM HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAATKAAHMNHF 120

Query: 121 SSTHTNNNN-SSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQE 180
           SST TNNNN SSSSSSSSSSSSDAT+ELTEIVQLPSLGTS+DGG    GGE VFFDSGQE
Sbjct: 121 SSTDTNNNNSSSSSSSSSSSSSDATEELTEIVQLPSLGTSFDGGG---GGELVFFDSGQE 180

Query: 181 LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 210

BLAST of Carg08281 vs. ExPASy Swiss-Prot
Match: Q9LYD3 (Dehydration-responsive element-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=DREB3 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-52
Identity = 126/226 (55.75%), Postives = 151/226 (66.81%), Query Frame = 0

Query: 2   SRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLG 61
           S +++ +    + +  KK KR RD+   KHP+YRGVRMRNWGKWVSEIREPRKKSRIWLG
Sbjct: 21  SESDSVSDKSKAEQSEKKTKRGRDS--GKHPVYRGVRMRNWGKWVSEIREPRKKSRIWLG 80

Query: 62  TFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHFS 121
           TFP+PEMAARAHDVAALSIKG AAILNFP+L    PRPVSL+PRD+Q AA KAAHM+  +
Sbjct: 81  TFPTPEMAARAHDVAALSIKGTAAILNFPELADSFPRPVSLSPRDIQTAALKAAHMEPTT 140

Query: 122 STHTNNNNSSSSSSSSSSSS---------DATDELTEIVQLPSLGTSYDGGDGGFGGEFV 181
           S  ++ ++SSS SS+SS  S           ++EL EIV+LPSLG SYD      G EFV
Sbjct: 141 SFSSSTSSSSSLSSTSSLESLVLVMDLSRTESEELGEIVELPSLGASYDVDSANLGNEFV 200

Query: 182 FFDSGQELESGCWMYTPPWVQSLEDCDNDDYGK------FEGLLWD 213
           F+DS         +Y PPW QS E    D+YG         GL WD
Sbjct: 201 FYDSVDYC-----LYPPPWGQSSE----DNYGHGISPNFGHGLSWD 235

BLAST of Carg08281 vs. ExPASy Swiss-Prot
Match: Q39127 (Ethylene-responsive transcription factor TINY OS=Arabidopsis thaliana OX=3702 GN=TINY PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.7e-48
Identity = 113/184 (61.41%), Postives = 133/184 (72.28%), Query Frame = 0

Query: 15  EPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD 74
           + N++ K+    +  KHP+YRGVR RNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD
Sbjct: 17  QENEEEKKKPVKDSGKHPVYRGVRKRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD 76

Query: 75  VAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHM---QHFSS----THTNN 134
           VAALSIKG +AILNFPDL    PRP SL+PRD+Q AA KAAHM   Q FSS    T +++
Sbjct: 77  VAALSIKGASAILNFPDLAGSFPRPSSLSPRDIQVAALKAAHMETSQSFSSSSSLTFSSS 136

Query: 135 NNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQELESGCWMY 192
            +SSS  S  SSS+  ++EL EIV+LPSLG+SYDG     G EF+F DS     +  W Y
Sbjct: 137 QSSSSLESLVSSSATGSEELGEIVELPSLGSSYDGLT-QLGNEFIFSDS-----ADLWPY 194

BLAST of Carg08281 vs. ExPASy Swiss-Prot
Match: Q9M080 (Ethylene-responsive transcription factor ERF043 OS=Arabidopsis thaliana OX=3702 GN=ERF043 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-42
Identity = 110/195 (56.41%), Postives = 130/195 (66.67%), Query Frame = 0

Query: 27  EDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSIKGKAAI 86
           E++K P+YRGVRMR+WGKWVSEIREPRKKSRIWLGTFP+ EMA RAHDVAA+SIKG +AI
Sbjct: 12  ENNKQPVYRGVRMRSWGKWVSEIREPRKKSRIWLGTFPTAEMAMRAHDVAAMSIKGTSAI 71

Query: 87  LNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQ----------HFSSTHTNNNNSSSSSS- 146
           LNFP+L  LLPRPVSL+PRDV+AAA KAA M             S T T+N  S SS S 
Sbjct: 72  LNFPELSKLLPRPVSLSPRDVRAAATKAALMDFDTTAFRSDTETSETTTSNKMSESSESN 131

Query: 147 -----SSSSSSDAT--------DELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQELES 198
                SSSS S  T        D+L EIV+LPSLGTS +  +     EFV FDS ++L  
Sbjct: 132 ETVSFSSSSWSSVTSIEESTVSDDLDEIVKLPSLGTSLNESN-----EFVIFDSLEDL-- 191

BLAST of Carg08281 vs. ExPASy Swiss-Prot
Match: Q52QU1 (Ethylene-responsive transcription factor ERF042 OS=Arabidopsis thaliana OX=3702 GN=ERF042 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 8.2e-37
Identity = 106/221 (47.96%), Postives = 135/221 (61.09%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           M  +    T CSS +  +K K        +  +YRG RMR+WGKWVSEIREPRKKSRIWL
Sbjct: 1   MVDSHGSDTECSSKKKKEKTK--------EKGVYRGARMRSWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAA----- 120
           GTFP+ EMAARAHDVAALSIKG +AILNFP+L   LPRPVSL+ +D+QAAAA+AA     
Sbjct: 61  GTFPTAEMAARAHDVAALSIKGSSAILNFPELADFLPRPVSLSQQDIQAAAAEAALMDFK 120

Query: 121 ----HMQHFS---------------STHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSL 180
               H+Q  S               S+ +++ +SSSSSSSSSSSS  + EL +IV+LPSL
Sbjct: 121 TVPFHLQDDSTPLQTRCDTEKIEKWSSSSSSASSSSSSSSSSSSSMLSGELGDIVELPSL 180

Query: 181 GTSYDGGDGGFGGEFVFFDSGQELESGCWMYTPPWVQSLED 198
                  +     +   +DS + L S      PPW+ + E+
Sbjct: 181 -------ENNVKYDCALYDSLEGLVS-----MPPWLDATEN 201

BLAST of Carg08281 vs. ExPASy Swiss-Prot
Match: O80654 (Ethylene-responsive transcription factor ERF037 OS=Arabidopsis thaliana OX=3702 GN=ERF037 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.0e-36
Identity = 97/178 (54.49%), Postives = 112/178 (62.92%), Query Frame = 0

Query: 21  KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSI 80
           KR+R       P YRGVRMR WGKWVSEIREPRKKSRIWLGTF +PEMAARAHD AAL+I
Sbjct: 35  KRVR-----SDPGYRGVRMRTWGKWVSEIREPRKKSRIWLGTFSTPEMAARAHDAAALTI 94

Query: 81  KGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQ---------------------- 140
           KG +A+LNFP+L   LPRP S +PRDVQAAAA AA M                       
Sbjct: 95  KGTSAVLNFPELATYLPRPASSSPRDVQAAAAVAAAMDFSPSSSSLVVSDPTTVIAPAET 154

Query: 141 HFSSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS 177
             SS+  +   SSS S SS  ++   +EL+EIV+LPSL TSYD        EFV+ DS
Sbjct: 155 QLSSSSYSTCTSSSLSPSSEEAASTAEELSEIVELPSLETSYDES----LSEFVYVDS 203

BLAST of Carg08281 vs. ExPASy TrEMBL
Match: A0A6J1HCS5 (dehydration-responsive element-binding protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111462803 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.0e-111
Identity = 205/213 (96.24%), Postives = 205/213 (96.24%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN TTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNENATTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAA M HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAARMHHF 120

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SSTHTNNNN     SSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL
Sbjct: 121 SSTHTNNNN-----SSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 208

BLAST of Carg08281 vs. ExPASy TrEMBL
Match: A0A6J1K785 (dehydration-responsive element-binding protein 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491786 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.3e-103
Identity = 200/214 (93.46%), Postives = 202/214 (94.39%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN  TTCSSPEPNKK KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNEN-ATTCSSPEPNKKLKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAA KAAHM HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAATKAAHMNHF 120

Query: 121 SSTHTNNNN-SSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQE 180
           SST TNNNN SSSSSSSSSSSSDAT+ELTEIVQLPSLGTS+DGG    GGE VFFDSGQE
Sbjct: 121 SSTDTNNNNSSSSSSSSSSSSSDATEELTEIVQLPSLGTSFDGGG---GGELVFFDSGQE 180

Query: 181 LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 LESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 210

BLAST of Carg08281 vs. ExPASy TrEMBL
Match: A0A6J1K953 (dehydration-responsive element-binding protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491786 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.2e-103
Identity = 200/217 (92.17%), Postives = 202/217 (93.09%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN  TTCSSPEPNKK KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNEN-ATTCSSPEPNKKLKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAA KAAHM HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAATKAAHMNHF 120

Query: 121 SSTHTNNNN----SSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS 180
           SST TNNNN    SSSSSSSSSSSSDAT+ELTEIVQLPSLGTS+DGG    GGE VFFDS
Sbjct: 121 SSTDTNNNNSSSSSSSSSSSSSSSSDATEELTEIVQLPSLGTSFDGGG---GGELVFFDS 180

Query: 181 GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 GQELESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 213

BLAST of Carg08281 vs. ExPASy TrEMBL
Match: A0A6J1K578 (dehydration-responsive element-binding protein 3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491786 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.5e-102
Identity = 196/213 (92.02%), Postives = 198/213 (92.96%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN  TTCSSPEPNKK KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNEN-ATTCSSPEPNKKLKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAA KAAHM HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAATKAAHMNHF 120

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SST TNNNN    SSSSSSSSDAT+ELTEIVQLPSLGTS+DGG    GGE VFFDSGQEL
Sbjct: 121 SSTDTNNNN----SSSSSSSSDATEELTEIVQLPSLGTSFDGGG---GGELVFFDSGQEL 180

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 205

BLAST of Carg08281 vs. ExPASy TrEMBL
Match: A0A6J1K2D0 (dehydration-responsive element-binding protein 3-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111491786 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.7e-101
Identity = 195/213 (91.55%), Postives = 197/213 (92.49%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           MSRNEN  TTCSSPEPNKK KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL
Sbjct: 1   MSRNEN-ATTCSSPEPNKKLKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHF 120
           GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAA KAAHM HF
Sbjct: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAATKAAHMNHF 120

Query: 121 SSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQEL 180
           SST TNNNN     SSSSSSSDAT+ELTEIVQLPSLGTS+DGG    GGE VFFDSGQEL
Sbjct: 121 SSTDTNNNN-----SSSSSSSDATEELTEIVQLPSLGTSFDGGG---GGELVFFDSGQEL 180

Query: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 214
           ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY
Sbjct: 181 ESGCWMYTPPWVQSLEDCDNDDYGKFEGLLWDY 204

BLAST of Carg08281 vs. TAIR 10
Match: AT5G11590.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 9.8e-54
Identity = 126/226 (55.75%), Postives = 151/226 (66.81%), Query Frame = 0

Query: 2   SRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLG 61
           S +++ +    + +  KK KR RD+   KHP+YRGVRMRNWGKWVSEIREPRKKSRIWLG
Sbjct: 21  SESDSVSDKSKAEQSEKKTKRGRDS--GKHPVYRGVRMRNWGKWVSEIREPRKKSRIWLG 80

Query: 62  TFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQHFS 121
           TFP+PEMAARAHDVAALSIKG AAILNFP+L    PRPVSL+PRD+Q AA KAAHM+  +
Sbjct: 81  TFPTPEMAARAHDVAALSIKGTAAILNFPELADSFPRPVSLSPRDIQTAALKAAHMEPTT 140

Query: 122 STHTNNNNSSSSSSSSSSSS---------DATDELTEIVQLPSLGTSYDGGDGGFGGEFV 181
           S  ++ ++SSS SS+SS  S           ++EL EIV+LPSLG SYD      G EFV
Sbjct: 141 SFSSSTSSSSSLSSTSSLESLVLVMDLSRTESEELGEIVELPSLGASYDVDSANLGNEFV 200

Query: 182 FFDSGQELESGCWMYTPPWVQSLEDCDNDDYGK------FEGLLWD 213
           F+DS         +Y PPW QS E    D+YG         GL WD
Sbjct: 201 FYDSVDYC-----LYPPPWGQSSE----DNYGHGISPNFGHGLSWD 235

BLAST of Carg08281 vs. TAIR 10
Match: AT5G25810.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 193.4 bits (490), Expect = 1.9e-49
Identity = 113/184 (61.41%), Postives = 133/184 (72.28%), Query Frame = 0

Query: 15  EPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD 74
           + N++ K+    +  KHP+YRGVR RNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD
Sbjct: 17  QENEEEKKKPVKDSGKHPVYRGVRKRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHD 76

Query: 75  VAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHM---QHFSS----THTNN 134
           VAALSIKG +AILNFPDL    PRP SL+PRD+Q AA KAAHM   Q FSS    T +++
Sbjct: 77  VAALSIKGASAILNFPDLAGSFPRPSSLSPRDIQVAALKAAHMETSQSFSSSSSLTFSSS 136

Query: 135 NNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQELESGCWMY 192
            +SSS  S  SSS+  ++EL EIV+LPSLG+SYDG     G EF+F DS     +  W Y
Sbjct: 137 QSSSSLESLVSSSATGSEELGEIVELPSLGSSYDGLT-QLGNEFIFSDS-----ADLWPY 194

BLAST of Carg08281 vs. TAIR 10
Match: AT4G32800.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 9.2e-44
Identity = 110/195 (56.41%), Postives = 130/195 (66.67%), Query Frame = 0

Query: 27  EDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSIKGKAAI 86
           E++K P+YRGVRMR+WGKWVSEIREPRKKSRIWLGTFP+ EMA RAHDVAA+SIKG +AI
Sbjct: 12  ENNKQPVYRGVRMRSWGKWVSEIREPRKKSRIWLGTFPTAEMAMRAHDVAAMSIKGTSAI 71

Query: 87  LNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQ----------HFSSTHTNNNNSSSSSS- 146
           LNFP+L  LLPRPVSL+PRDV+AAA KAA M             S T T+N  S SS S 
Sbjct: 72  LNFPELSKLLPRPVSLSPRDVRAAATKAALMDFDTTAFRSDTETSETTTSNKMSESSESN 131

Query: 147 -----SSSSSSDAT--------DELTEIVQLPSLGTSYDGGDGGFGGEFVFFDSGQELES 198
                SSSS S  T        D+L EIV+LPSLGTS +  +     EFV FDS ++L  
Sbjct: 132 ETVSFSSSSWSSVTSIEESTVSDDLDEIVKLPSLGTSLNESN-----EFVIFDSLEDL-- 191

BLAST of Carg08281 vs. TAIR 10
Match: AT2G25820.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 5.8e-38
Identity = 106/221 (47.96%), Postives = 135/221 (61.09%), Query Frame = 0

Query: 1   MSRNENGTTTCSSPEPNKKPKRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWL 60
           M  +    T CSS +  +K K        +  +YRG RMR+WGKWVSEIREPRKKSRIWL
Sbjct: 1   MVDSHGSDTECSSKKKKEKTK--------EKGVYRGARMRSWGKWVSEIREPRKKSRIWL 60

Query: 61  GTFPSPEMAARAHDVAALSIKGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAA----- 120
           GTFP+ EMAARAHDVAALSIKG +AILNFP+L   LPRPVSL+ +D+QAAAA+AA     
Sbjct: 61  GTFPTAEMAARAHDVAALSIKGSSAILNFPELADFLPRPVSLSQQDIQAAAAEAALMDFK 120

Query: 121 ----HMQHFS---------------STHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSL 180
               H+Q  S               S+ +++ +SSSSSSSSSSSS  + EL +IV+LPSL
Sbjct: 121 TVPFHLQDDSTPLQTRCDTEKIEKWSSSSSSASSSSSSSSSSSSSMLSGELGDIVELPSL 180

Query: 181 GTSYDGGDGGFGGEFVFFDSGQELESGCWMYTPPWVQSLED 198
                  +     +   +DS + L S      PPW+ + E+
Sbjct: 181 -------ENNVKYDCALYDSLEGLVS-----MPPWLDATEN 201

BLAST of Carg08281 vs. TAIR 10
Match: AT1G77200.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.9e-37
Identity = 97/178 (54.49%), Postives = 112/178 (62.92%), Query Frame = 0

Query: 21  KRIRDTEDSKHPLYRGVRMRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSI 80
           KR+R       P YRGVRMR WGKWVSEIREPRKKSRIWLGTF +PEMAARAHD AAL+I
Sbjct: 35  KRVR-----SDPGYRGVRMRTWGKWVSEIREPRKKSRIWLGTFSTPEMAARAHDAAALTI 94

Query: 81  KGKAAILNFPDLVHLLPRPVSLAPRDVQAAAAKAAHMQ---------------------- 140
           KG +A+LNFP+L   LPRP S +PRDVQAAAA AA M                       
Sbjct: 95  KGTSAVLNFPELATYLPRPASSSPRDVQAAAAVAAAMDFSPSSSSLVVSDPTTVIAPAET 154

Query: 141 HFSSTHTNNNNSSSSSSSSSSSSDATDELTEIVQLPSLGTSYDGGDGGFGGEFVFFDS 177
             SS+  +   SSS S SS  ++   +EL+EIV+LPSL TSYD        EFV+ DS
Sbjct: 155 QLSSSSYSTCTSSSLSPSSEEAASTAEELSEIVELPSLETSYDES----LSEFVYVDS 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029718.12.9e-116100.00Dehydration-responsive element-binding protein 3, partial [Cucurbita argyrosperm... [more]
KAG6598779.11.6e-11498.16Dehydration-responsive element-binding protein 3, partial [Cucurbita argyrosperm... [more]
XP_022962316.18.2e-11196.24dehydration-responsive element-binding protein 3-like [Cucurbita moschata][more]
XP_023546365.11.8e-10593.43ethylene-responsive transcription factor TINY-like [Cucurbita pepo subsp. pepo][more]
XP_022996590.14.8e-10393.46dehydration-responsive element-binding protein 3-like isoform X2 [Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
Q9LYD31.4e-5255.75Dehydration-responsive element-binding protein 3 OS=Arabidopsis thaliana OX=3702... [more]
Q391272.7e-4861.41Ethylene-responsive transcription factor TINY OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M0801.3e-4256.41Ethylene-responsive transcription factor ERF043 OS=Arabidopsis thaliana OX=3702 ... [more]
Q52QU18.2e-3747.96Ethylene-responsive transcription factor ERF042 OS=Arabidopsis thaliana OX=3702 ... [more]
O806544.0e-3654.49Ethylene-responsive transcription factor ERF037 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HCS54.0e-11196.24dehydration-responsive element-binding protein 3-like OS=Cucurbita moschata OX=3... [more]
A0A6J1K7852.3e-10393.46dehydration-responsive element-binding protein 3-like isoform X2 OS=Cucurbita ma... [more]
A0A6J1K9535.2e-10392.17dehydration-responsive element-binding protein 3-like isoform X1 OS=Cucurbita ma... [more]
A0A6J1K5787.5e-10292.02dehydration-responsive element-binding protein 3-like isoform X3 OS=Cucurbita ma... [more]
A0A6J1K2D01.7e-10191.55dehydration-responsive element-binding protein 3-like isoform X4 OS=Cucurbita ma... [more]
Match NameE-valueIdentityDescription
AT5G11590.19.8e-5455.75Integrase-type DNA-binding superfamily protein [more]
AT5G25810.11.9e-4961.41Integrase-type DNA-binding superfamily protein [more]
AT4G32800.19.2e-4456.41Integrase-type DNA-binding superfamily protein [more]
AT2G25820.15.8e-3847.96Integrase-type DNA-binding superfamily protein [more]
AT1G77200.12.9e-3754.49Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 56..72
score: 61.71
coord: 34..45
score: 71.21
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 33..96
e-value: 6.5E-34
score: 128.7
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 32..82
e-value: 1.7E-13
score: 50.6
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 33..90
score: 23.221937
IPR001471AP2/ERF domainCDDcd00018AP2coord: 32..92
e-value: 2.90137E-32
score: 109.669
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 33..91
e-value: 9.6E-24
score: 85.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..144
NoneNo IPR availablePANTHERPTHR31985ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATEDcoord: 11..213
NoneNo IPR availablePANTHERPTHR31985:SF220DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 11..213
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 33..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08281-RACarg08281-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity