Carg08005 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08005
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMitochondrial intermediate peptidase, putative
LocationCarg_Chr04: 1694613 .. 1702604 (-)
RNA-Seq ExpressionCarg08005
SyntenyCarg08005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGCCGCGGTATGTTGAGCCTGATCAGAAAAACCACCCGGAAGGTCAGTTCGGGAGCCGTTCTAAAATCATGCTACTTGAATTGCCTTGGCTCCCGTTCGATCCACGCCGCTGCCTCGGCTCCGCACGAAGGTCCTGCTACCGGTCTCTATGGCTTTGAGCAGCTGAAGTCGCCTAAAGGCTTCCGGCGTTTCGTCGATGAAGCCATTGAGAGGTAACGAGATAATTGCTCTAAGTTGAATGTCTTTAATGCTTGAGAGACGAGACTCAATTTGGAAGCCTAAGTGAAGAAACTGAAAGTATTCTGATATTGCATACAATAACAACATTAATTCCGTCAGCGTTTCTTGGTGAACAAGATAATGTGTGGACTAAGTTACTACGATTTTTCTTACAATTTTAATTGTTTTGTTGCTCATTATCATCGGCTAGCTTAACACTAAAAACAATTGAGCTTAATTATGTATCTGACTAATTAGAGGCGTTCCACCTCGTTTCCATGGTAATGATTATGAGCTGAACTTAAATAGCTCTTTGTTCTTTTAGAAAAAAAGAAGCTTGGTTGATGCTATAATTGCTATGGCCAATGGTAATAAGAATGTAGGTAATGCGTAATGGTATGTAAGAAAATGAAACAGTTCTTATTGTCTTAATTCTAGGAGGATCTTTTGCAATACACATTGACCTTTTTGATAAACGAGATCAAAAGCTATGTGCTTTGCCTTCTTTAAAATTAAGGCAGTTCGGAAACTGGGCTTTCATCTATGGGTTTCTTTCTTTGAATTGCAAGAGTGCAGAAAAGTGCAAGATACAAAGGATTTTGAAATAAAAATCATCTAGTCTAGTATTATATGCACTATCATTTGGAAATTCGAACATCGTTCATACGATTTTTTTTAGTTCAACAATATTTGAGGAAGGATTCAAACGGCTGACTTGTTGGTCGAGGGTACATGTCAATTACTCATTCATTTTCTAGAATGTCAGCTGACTTGAATACATGGATGCTCTAAAAAAAACTCATCAATATTTTACGAAAAATCTCTAAATTCATTGATAGCAAGGAGCCTTGAAACCTTTATCTAATTCTAACTGCAAGAAAAAAAAATTGCATTCTTCTTTTAAAAACTAATTTCTCTGATTAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCATGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGGGTAGGTTTAGAATTTAGGATTTACTTTAAGTTCTTATTTTGAACTATCTAGCAGATATATTTCCACTTCTATATAAAAGTTCTTTGTTTAATTGTGGAATTTATTGATTAGGTATGCTCTGTTTTTGATTCTGCAGAACTCTGTAGGCAAACTCATCCAGACAGGTTTGTACTTTTCTGATGTTTTTTTGACACCTTGGTTATAGTTACTGTCTTTCATATTGCTTTGCCAGTCTGTTTGGCTTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGTAATAGGGAATTCGTGGAGGAGGCAAACAAGGCAGCCATGAGAATGAGTGAATATTTTCATGTGAGGAGCCCAGCACACCATATCTTGATTTTTTTTTTTTTTTTTTTGTGAGTTGTCATTATTACTGATTTACTAACTAATTGAGTGTCACCATCATGTCTGACTTTACCTTCAGTGAAAAGCAAACATGTTTTCCGGAATTGTCTATTCTTCTCTAGTTCTGGACTCTAGTGCTAAAATATATAAAAAAACTTAAGAAATAATAATCTGCTTAAGTGATAAAATGTGCTGTATTCGCAGAACTGAATTGCCTCTTTTATTATATAAACCTTCTTTAGGTAGTAACTTGGAAACCTTTGTGAAGGAGGAAAAATAACTCTTGTGGACAGTGGGACTTGACAGACTCAACAAACAACCTAGTTTTTAGGCTTCTCCATAAATGTTCTCTCTCATAATTCAATAGAATATGGAAAATTTTATAAGTTAATACTTGATTAGTTATTAATAATCTCTGTCTCTCTCTTCAACATCACTTTGAACCTGCTCAACTTTTGTGATAGTTATTATGAGATAGCAAGTTTTAAATTTTCTACTTGATTTTCATCTGTTATTTTATAAATAGCTTGAACCTACTGAAACTGAAAAGTACTTAGCATAAGTAACATGATCCTACTGAATTGAAAAGTATTTAACATTTTAATTTGAACAAAAAAGGATGAGAACTTTTAGGTGTAATTATATTGAAAAAAAATATGGGAAGAACTTTGTATTTGTTTCTAAACAAAAAGATATTGTTATTGGTAATTTTCTTGCTGTTTGAAAACTGTGTCCAAAAATTGATTCATGTTATCTTTCTGTTTATAGTTTCTCAATACAAATCATACTTTGTATAATGCTGTTAAAAAAGCTGAGCGTGAGGCTCATCTACTTACACGTGAAGCGCATATGGCTGCTCATTTCCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGGTACTTTTTGTGTATAATTCACTCCGCTGCTTCCTACTCATGCTTCTGCATTTAAAAATTAACACCTTAATTATGCTTATGCAGAGAGATTGGATCGAGTAAATCAGCTGAGTATTGAAATTTCTCAGCTCTGTCAGGAGTAAGTTGCATGTAATATTCACTTAATTGTGTTTGCTTGCTATTCACATCATTTTCATGCTTCTTCCAATAATATTTTCTGCATATCAATAAACAAAACGGAGTCACTTTCTGTCACTCTAAACTGTACGTAAACGGAGTCACTTTGATAATGCTCTTAACATTCAGAGCGATTGTAAGTGTTTGATGAATTCTCTTCTTTATCACTATTTGAGAAACCTCACTATATTAATACTGATATATGCAAGTATTTCATGACAACCCAGACCAGTATTTCTTACATATAGGAGGAAGGAAGAGAAAAAGAAAAATGTTCCATGTTTGTTAATTTTGTTGAATATGCTTCATTTGGTCAGGTACAAAGAAAATATCGTCATTGATCCAGGCTCTGTGGATATATTTCCACCAATGCGTGTGCCAAACAACTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTAAATCATTTGGATCGATAAGTAGCATGAAAGAAAAGGGATTTCGATTAATGACTGATGCAGATTCCCTATCTTCTATTCTGCAATATACATCAGATGATGAGGTGAATAACTCTGTTGTGCATGATGATAGATAGGATGTGTTTTCATTGGCTTGGGATAATTTTAAAGGATGTGGAGGCTTCCATAAATTCTTGTAGGATAAAGTTCTATGATGCATCTTTTTTAGGTCAGATGGATTTAGATATCTGAGGAAGGAAATCTTATAAGATGATGACACTTTTCGTAAATTATGGTTTGCTAAAGCCAAATGTTTCATCCTTGGTCTTGATCCTTTTTCTGATCAACAATTACTTTTTTCTTTGCACTTGAATATATTCTCAGGTTAGGAAAATGGCTTATGTCAAGGGAAATTCAAGTCCTTGTGCTAACCTCGATGTTCTTGATAAGATTATTGCCACTCGTCATGAGCTAGCTCAGGTTATTCATTTTTATTTTCACTCTCCTGAAATTTGTAAAGAATAATGAATTATCTAATATTTCTTCCTTAAGTTGCCATTTGTTTAATACTGAGTTCTTAAAAAAAATTCTCTCCTTGAAATGAGTCAATCTAATCTTGTGGTGCAAAAAAATTCATTCTTTTATGAGGCCATGTGAACTTCCGTCTCACTTAATCTGAGGTCTTATTTTAGAATTGTTTCCTGCTGAAGATTTATATTTCATCGTTCATGGCAACGATTTGTACAATTTTCTAATGGGCTAGGGAATAAAAGTAGACTTGAATCAAATTAATGAGGAAGGGCTTACAACACTCCCAGACTTTCAAGAGGTCTATTTCTCTCAAAAATAAAGCTCGAGCTCTCTAGCTCCTGAATTTACAAAATTTATATGAAAAGACTTGTACGGCCTTCTCTTACGATGACTTTTCAGCTCAATTATTTCCTCTTGGCTTTGAGCCTTCTTAACCTTCCTCAGTCATGTGTTAACCAAATAAGCTTCTTCTATTTCAATCAATACCAGTCGCTTGATATTTTTTTCATCTACACAATTGCTGTGGAACTTTGAGAAAATAAATTTAGAAACATATTCGATTAATAGAACAGAATTGTATTATAAATCTACCAGATTCCTTGAGATGAAATTTCTGTTTATTCCAATTTGTGAAAAAGGTTCTGACAAATAGTTTCCTACATATGGAAAAATAATCATGTCACGGTGAATTACCACAATGTTTTAGTCTTTTGCTTAATGTTGGCTATGCAGATATTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACATGGCTTCATCTCCAGCTGTAGTAGTGTCATTTTTGCAAGAGTTGAGCAATTTGGTCCGTCCCAGTGCTGATGAGGTACTACAATTTTTGTTTCAAGATGTTGCAGTTTGCAAGGCATGGAAGATTTGTTTCTGTTTCGTCATAACTTGTTGAAATTGTGGTTTCATGATTTAATGAGAAGTTTTAACCACCACCAGGAGTTCAATCAAATCAGGGAATTCAAGTTAAAGAAGCGTTTTAATAAGTTTGAAGATTTAGAACCATGGGATGAGGCTTATTATACATCAATGATGAAATCTACCGCGTATAATCTCAGTTCACCGGCAAGTTACATCTTCTATTTCTTTCTTATTTCTCTCTCTCTTTTTTTTTTCAAATGAAAAATGTGTCGAGGTTGTTCCGTCTTCACATTGTTAAGTGGCGTTCTTCTACACTTTTTTTTTTAGTTTTTCCTTTTGCCTACTTCGTTTCTCTCACGGTATCCATTTTCTCCCAACTGCTAGTATTCAGTTTTCTTATGGTTCCCTTTTCTTTTTATAGGTCATATCATCATACTTTCCTTTATCACAATGTATTGAAGGTTTGAAAACTCTCGTGAACTCATTGTTTGGCGCATCATTTAATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCCGACGTGCTTAAATTGTCTCTTCATCATCCTGGAGAGGTACATACTAGATAAGTGAACTATAGATTTTTCTTCATTAGTTATAGCTTGTAATTGAATTAGACTGTTTGTGCACTGAACCTTTGGCTAATAAGTTTTTGTACACTGAACCTTTGGCTAATAAGTTTTCTGACACACTAGTTCCCCTGATACGTAGTGGAGACTGTGGTGATGAGCTTTTGTTATTCATATATTCATGTATAAATTGTTATGGTTGAGATCAAAGATTTAACCACACGTCAGTAGTCTTGGAATGAGAAACTATTATATCAAGCCTTAAAAAGATGTACATGACTAAGTGTCTCTTGAATCAAATCCTTCGGGGCTCCGTTTATATATTGTGTGAGTTATGGTGCTCGGTTCCAACACCACCACCCCCCCCCCCCCCCCCCCCAAACATCATAAGTACTCCCCTAAACTCATAGTGCCACCATATTTCTGTTTCAGGGTGACTTGGGATACTTATACCTAGATTTGTACTCGAGAAAAGGAAAGTATCCTGGTTGTGCTCATTTTGCAATTCAGGGAGGCTGCAAGGTTTCAGAAATGGAATACCAACTTCCTGTATGTATTAGATAGCACTTTGTACGTGTTAGCACTTTTGATTTCTATAATACTTCCTACTACAGCTATTCGGCTGTAACTGTATCTGATCAAACATTTTTGTGATGCTTCAATTCTTGTTTTTGCAAAATTGGATAGACACGTAATCCTCTTTTAGCTATCACATACCATGTTGTAATCAAGAATGTGAAAAATCTACTAATGCTTATAGGCTAAGATAGAGAATGGATTGCATGGTGATGGGTTTGATATTCTTATTATTACGATGGGGTATGCGTTGGTTGAAAATGTGCTCATGATTTTTGTTTGCTAATGTCAGGCTAATTAGATGATCTTGCATATTCTCTTTCAGCTTGTAACCAAAAGTTGATTATTCAATGCTAATAATATATCCATTCGCGAAGGATCCTAAGGAAAAGTTTTTTAGAAATGTAAAGGAGTAGACTAATTTGGTGACCTCCCAAATCAGAAGTTTGTATTGTTTATGTGCAGGTTGTAGCTCTTGTTTGCAATTTTTCTGGATCGCGTGAACGATCAAATGTGAGGCTTAATCATTCGGAAGTTGAAACTCTTTTCCACGAGTTTGGGCATGCCCTTCATTCCCTGCTTTCAAGAACAGTATGTGCTGATTCTGTACTCTTGAACAGACGATCCTTTTGTTACTTTTAAATATATAATTCATCAATCCTCATCTACTGCAGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTGAGTAAAAATGCAGTTTATTTGCTTGCCTTTTGGTTTACGAAGATCATATAACGGTTTTGAACCACGTCTATGCTTAAATTATTCTTCTAGCTAAGTTGTGCAGTGTTTGTACTACGGCCTTTATAAGAAAGACGTCCATTAATCTATATCAAATATGAACATTAAATATGGGATAAAAAATGTCCAAAATATCCTTGTCTAAATTTTTATTAGGAGATAATAAATCAACAAATTTATATAATTCTTCTCTTATAAATATGTAATCTTGTACGTATAACTTGACAAAACCCTTCTCTCAGTCTTGTTTCCATATCAGCTGTGTTTGTTGTTGTTTAATAATAGCCGTCACCACTATTGGATGACTAGGTACTACGCGTCAGATTATCGTGTTTTGAGAACATTTGCTAAGCACTATTCAACTGGTGAAGTAATTCCTGAAAAGCTTGTAAAGTCGATGCAGGGTGCCAAAAAGATGTTTGCAGCCACTGAACTGCAGCGTCAGGTGACATGTTGATGGTTGTTGTTACCTGGTATATCTTATAATTTTATCTGATCCAACCAACCAATTTTTACTTGGTTTTTTGATTTCCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGTGAAAGATTAACTTCAGAGAGAGACACTAGTTCTCTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTTTCACCTCCTGACTTATGGTGCAGGTTGCAAAAGTTGATCTATTCTTGTGTTTGAGTGATATATTTTTGCTTCTCAATTTGATATGAGATCTTTAAGCTTCTGTGACTGATCTTGTAACAGCTCAAGCCCACCGCTAGCAGATATTATCTTCTTTGGATTTTTGTCTTAAGGTTTTTAAAACTTGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAAAATGTTTTGTTCTCCTCTCCAACCGATGTGAGATCTCACAATCCACCCACCTTCGGGACAGCGTCCTCTCTAGTACTCGTTCTTTTCTCCAATCAATGTGGGACTCCCCAATCCATCTCCAAGTCAATGAAGCAGATGCTAATACTTGCATAGTTTGTAATTCAAAATAACCAAAACACCTGTCTCCTCACCCACACCCCGAATAGCAGTTTATATATACTATAACGTTTTTACTTAATTTTCCCTCGTTTCTTTCTGGTGCGAGACATTCACAGGTATGCTTTTTACAGGTTACTACAGCTACCTGTACGCCAAATGTTTTGCCGCAACCATATGGGAGAAGCTTTGTCAGGAAGATCCTCTTTCAAGAAGCACAGGAACTGCCTTGAGAACAAAGTTCTTACAACATGGAGGCTCCAAAGAACCAGTTGATTTACTAAAGGATCTTGTGGGAGATGGGATCATACGGTATTGTGATGGAGGAGTTATTCCTGATATAACCAGTCTTTGCAAGGAAATGGATATCAGTGGAAAACTTGTAGTGTAGCCACAAGCAAGCCCTTTCGCCATCCTTTTTCCCCCTTAATTATT

mRNA sequence

CCGCCGCGGTATGTTGAGCCTGATCAGAAAAACCACCCGGAAGGTCAGTTCGGGAGCCGTTCTAAAATCATGCTACTTGAATTGCCTTGGCTCCCGTTCGATCCACGCCGCTGCCTCGGCTCCGCACGAAGGTCCTGCTACCGGTCTCTATGGCTTTGAGCAGCTGAAGTCGCCTAAAGGCTTCCGGCGTTTCGTCGATGAAGCCATTGAGAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCATGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGGGTATGCTCTGTTTTTGATTCTGCAGAACTCTGTAGGCAAACTCATCCAGACAGGGAATTCGTGGAGGAGGCAAACAAGGCAGCCATGAGAATGAGTGAATATTTTCATTTTCTCAATACAAATCATACTTTGTATAATGCTGTTAAAAAAGCTGAGCGTGAGGCTCATCTACTTACACGTGAAGCGCATATGGCTGCTCATTTCCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGAGAGATTGGATCGAGTAAATCAGCTGAGTATTGAAATTTCTCAGCTCTGTCAGGAGTACAAAGAAAATATCGTCATTGATCCAGGCTCTGTGGATATATTTCCACCAATGCGTGTGCCAAACAACTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTAAATCATTTGGATCGATAAGTAGCATGAAAGAAAAGGGATTTCGATTAATGACTGATGCAGATTCCCTATCTTCTATTCTGCAATATACATCAGATGATGAGGTTAGGAAAATGGCTTATGTCAAGGGAAATTCAAGTCCTTGTGCTAACCTCGATGTTCTTGATAAGATTATTGCCACTCGTCATGAGCTAGCTCAGATATTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACATGGCTTCATCTCCAGCTGTAGTAGTGTCATTTTTGCAAGAGTTGAGCAATTTGGTCCGTCCCAGTGCTGATGAGGAGTTCAATCAAATCAGGGAATTCAAGTTAAAGAAGCGTTTTAATAAGTTTGAAGATTTAGAACCATGGGATGAGGCTTATTATACATCAATGATGAAATCTACCGCGTATAATCTCAGTTCACCGGTCATATCATCATACTTTCCTTTATCACAATGTATTGAAGGTTTGAAAACTCTCGTGAACTCATTGTTTGGCGCATCATTTAATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCCGACGTGCTTAAATTGTCTCTTCATCATCCTGGAGAGGGTGACTTGGGATACTTATACCTAGATTTGTACTCGAGAAAAGGAAAGTATCCTGGTTGTGCTCATTTTGCAATTCAGGGAGGCTGCAAGGTTTCAGAAATGGAATACCAACTTCCTGTTGTAGCTCTTGTTTGCAATTTTTCTGGATCGCGTGAACGATCAAATGTGAGGCTTAATCATTCGGAAGTTGAAACTCTTTTCCACGAGTTTGGGCATGCCCTTCATTCCCTGCTTTCAAGAACAGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTACTACGCGTCAGATTATCGTGTTTTGAGAACATTTGCTAAGCACTATTCAACTGGTGAAGTAATTCCTGAAAAGCTTGTAAAGTCGATGCAGGGTGCCAAAAAGATGTTTGCAGCCACTGAACTGCAGCGTCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGTGAAAGATTAACTTCAGAGAGAGACACTAGTTCTCTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTTTCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTGTACGCCAAATGTTTTGCCGCAACCATATGGGAGAAGCTTTGTCAGGAAGATCCTCTTTCAAGAAGCACAGGAACTGCCTTGAGAACAAAGTTCTTACAACATGGAGGCTCCAAAGAACCAGTTGATTTACTAAAGGATCTTGTGGGAGATGGGATCATACGGTATTGTGATGGAGGAGTTATTCCTGATATAACCAGTCTTTGCAAGGAAATGGATATCAGTGGAAAACTTGTAGTGTAGCCACAAGCAAGCCCTTTCGCCATCCTTTTTCCCCCTTAATTATT

Coding sequence (CDS)

ATGTTGAGCCTGATCAGAAAAACCACCCGGAAGGTCAGTTCGGGAGCCGTTCTAAAATCATGCTACTTGAATTGCCTTGGCTCCCGTTCGATCCACGCCGCTGCCTCGGCTCCGCACGAAGGTCCTGCTACCGGTCTCTATGGCTTTGAGCAGCTGAAGTCGCCTAAAGGCTTCCGGCGTTTCGTCGATGAAGCCATTGAGAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCATGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGGGTATGCTCTGTTTTTGATTCTGCAGAACTCTGTAGGCAAACTCATCCAGACAGGGAATTCGTGGAGGAGGCAAACAAGGCAGCCATGAGAATGAGTGAATATTTTCATTTTCTCAATACAAATCATACTTTGTATAATGCTGTTAAAAAAGCTGAGCGTGAGGCTCATCTACTTACACGTGAAGCGCATATGGCTGCTCATTTCCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGAGAGATTGGATCGAGTAAATCAGCTGAGTATTGAAATTTCTCAGCTCTGTCAGGAGTACAAAGAAAATATCGTCATTGATCCAGGCTCTGTGGATATATTTCCACCAATGCGTGTGCCAAACAACTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTAAATCATTTGGATCGATAAGTAGCATGAAAGAAAAGGGATTTCGATTAATGACTGATGCAGATTCCCTATCTTCTATTCTGCAATATACATCAGATGATGAGGTTAGGAAAATGGCTTATGTCAAGGGAAATTCAAGTCCTTGTGCTAACCTCGATGTTCTTGATAAGATTATTGCCACTCGTCATGAGCTAGCTCAGATATTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACATGGCTTCATCTCCAGCTGTAGTAGTGTCATTTTTGCAAGAGTTGAGCAATTTGGTCCGTCCCAGTGCTGATGAGGAGTTCAATCAAATCAGGGAATTCAAGTTAAAGAAGCGTTTTAATAAGTTTGAAGATTTAGAACCATGGGATGAGGCTTATTATACATCAATGATGAAATCTACCGCGTATAATCTCAGTTCACCGGTCATATCATCATACTTTCCTTTATCACAATGTATTGAAGGTTTGAAAACTCTCGTGAACTCATTGTTTGGCGCATCATTTAATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCCGACGTGCTTAAATTGTCTCTTCATCATCCTGGAGAGGGTGACTTGGGATACTTATACCTAGATTTGTACTCGAGAAAAGGAAAGTATCCTGGTTGTGCTCATTTTGCAATTCAGGGAGGCTGCAAGGTTTCAGAAATGGAATACCAACTTCCTGTTGTAGCTCTTGTTTGCAATTTTTCTGGATCGCGTGAACGATCAAATGTGAGGCTTAATCATTCGGAAGTTGAAACTCTTTTCCACGAGTTTGGGCATGCCCTTCATTCCCTGCTTTCAAGAACAGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTACTACGCGTCAGATTATCGTGTTTTGAGAACATTTGCTAAGCACTATTCAACTGGTGAAGTAATTCCTGAAAAGCTTGTAAAGTCGATGCAGGGTGCCAAAAAGATGTTTGCAGCCACTGAACTGCAGCGTCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGTGAAAGATTAACTTCAGAGAGAGACACTAGTTCTCTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTTTCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTGTACGCCAAATGTTTTGCCGCAACCATATGGGAGAAGCTTTGTCAGGAAGATCCTCTTTCAAGAAGCACAGGAACTGCCTTGAGAACAAAGTTCTTACAACATGGAGGCTCCAAAGAACCAGTTGATTTACTAAAGGATCTTGTGGGAGATGGGATCATACGGTATTGTGATGGAGGAGTTATTCCTGATATAACCAGTCTTTGCAAGGAAATGGATATCAGTGGAAAACTTGTAGTGTAG

Protein sequence

MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Homology
BLAST of Carg08005 vs. NCBI nr
Match: KAG7030829.1 (putative mitochondrial intermediate peptidase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 720/720 (100.00%), Postives = 720/720 (100.00%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720

BLAST of Carg08005 vs. NCBI nr
Match: XP_022941716.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 713/720 (99.03%), Postives = 716/720 (99.44%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCL SRSIHAAASAPHEG ATGLYGFEQLKSPKGFRR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQI+EFKLKKRFNKFEDLEPW
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIKEFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL+S VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHP EGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSS+VAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720

BLAST of Carg08005 vs. NCBI nr
Match: XP_023518581.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1413.7 bits (3658), Expect = 0.0e+00
Identity = 708/720 (98.33%), Postives = 712/720 (98.89%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGA+LKSCYLNCL SRSIHAAASAPHEG ATGLYGFEQLKSPKGFRR
Sbjct: 1   MLSLIRKTTRKVSSGAILKSCYLNCLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLT EAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTHEAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSP ANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPHANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           Y+SYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW
Sbjct: 301 YKSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL+S VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHP EGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNF G
Sbjct: 421 VLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFYG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSS+VAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISG LVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGNLVV 720

BLAST of Carg08005 vs. NCBI nr
Match: XP_022972575.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 707/720 (98.19%), Postives = 712/720 (98.89%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCL SRSIHAAASAPHEG ATGLYGFEQLKSPKGF+R
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFQR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAE EAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEHEAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSP ANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPRANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL+S VISSYFPL+QCIEGLKTLVNSLFGASFNSI LAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLNSSVISSYFPLTQCIEGLKTLVNSLFGASFNSISLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHP EGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLT ERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSS+VAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDL+GDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720

BLAST of Carg08005 vs. NCBI nr
Match: KAG6600162.1 (Mitochondrial intermediate peptidase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1334.7 bits (3453), Expect = 0.0e+00
Identity = 678/720 (94.17%), Postives = 678/720 (94.17%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADE                       
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADE----------------------- 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
                              VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD
Sbjct: 361 -------------------VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 678

BLAST of Carg08005 vs. ExPASy Swiss-Prot
Match: F4KDA5 (Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OCT1 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 5.9e-273
Identity = 461/677 (68.09%), Postives = 547/677 (80.80%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G ATGLYGF+ LK+ KGF+RFV +AIERSGELV++IS MPSS E+I+AMDEIS+ VC V 
Sbjct: 28  GDATGLYGFDHLKTAKGFQRFVADAIERSGELVSYISGMPSSPEIIKAMDEISDTVCCVV 87

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMA 160
           DSAELCRQTHPDREFVEEANKAA+ M++Y H LNTNHTLY AVKKAE++++LLT+EA   
Sbjct: 88  DSAELCRQTHPDREFVEEANKAAIEMNDYLHHLNTNHTLYAAVKKAEQDSNLLTKEASRT 147

Query: 161 AHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNL 220
           AH LR+DFER GIHL  E+LD+VN L+  I QLC+E+ ENI  DPG VDIFP  R+P +L
Sbjct: 148 AHHLRMDFERGGIHLDPEKLDKVNNLTTNIFQLCREFSENIADDPGHVDIFPGSRIPRHL 207

Query: 221 HHLVKPIYRSSK-----SFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGN 280
           HHL+ P YRS+      S  S    K+KGFR+ TD  ++SSILQ+TSD+EVRKM Y++GN
Sbjct: 208 HHLLNPTYRSTSGGSRGSTRSAHKSKQKGFRINTDPRTVSSILQWTSDEEVRKMVYIQGN 267

Query: 281 SSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSA 340
           S P AN  VL+K+IA RHEL+Q++G  SYA+  V PN+A SP VV SFLQELS  V+P A
Sbjct: 268 SVPHANHGVLEKLIAARHELSQMMGCNSYADIMVEPNLAKSPKVVTSFLQELSKTVKPKA 327

Query: 341 DEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLK 400
           DEEF  IR+FK +K  N   +LEPWDE YYTSMMKS+  ++ + V++SYFPL QCIEGLK
Sbjct: 328 DEEFIAIRDFKREKCGNPSAELEPWDETYYTSMMKSSINDVDTAVVASYFPLPQCIEGLK 387

Query: 401 TLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFA 460
            LV SLFGA+F++IPLAPGESWHP+V+KLSLHHP EGDLGYLYLDLYSRKGKYPGCA FA
Sbjct: 388 VLVESLFGATFHTIPLAPGESWHPNVVKLSLHHPDEGDLGYLYLDLYSRKGKYPGCASFA 447

Query: 461 IQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 520
           I+GG K+SE EYQLPV+ALVCNFS + + S V+LNHSEVE LFHEFGHALHSLLSRT+YQ
Sbjct: 448 IRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNHSEVEVLFHEFGHALHSLLSRTDYQ 507

Query: 521 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAAT 580
           HFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HYSTGE IPEKLV S+QGA+ MFAAT
Sbjct: 508 HFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHYSTGETIPEKLVNSLQGARNMFAAT 567

Query: 581 ELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGA 640
           E+QRQ+ YALIDQ LFGE+  + RD S LVAELKRQ TSW HV+GTHW  RF HLL YGA
Sbjct: 568 EMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQHTSWNHVEGTHWYIRFSHLLNYGA 627

Query: 641 GYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIR 700
           GYYSYLYAKCFA+TIW+ +C+EDPLS +TGT LR KF +HGG+K+P +LL DL G  II 
Sbjct: 628 GYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREKFFKHGGAKDPAELLTDLAGKEIIS 687

Query: 701 YCDGGVIPDITSLCKEM 713
               G++P  T L  E+
Sbjct: 688 VHGEGIVPATTYLLNEL 704

BLAST of Carg08005 vs. ExPASy Swiss-Prot
Match: Q99797 (Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 1.2e-103
Identity = 227/679 (33.43%), Postives = 355/679 (52.28%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G   GL+G  +L +P+GF    ++A+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 60  GERRGLFGVPELSAPEGFHIAQEKALRKTELLVDRACSTPPGPQTVLIFDELSDSLCRVA 119

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHL---LTREA 160
           D A+  +  HP+  F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 120 DLADFVKIAHPEPAFREAAEEACRSIGTMVEKLNTNVDLYQSLQKLLADKKLVDSLDPET 179

Query: 161 HMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVP 220
              A     DFE +GIHL  E+  R   L+++I  L             S         P
Sbjct: 180 RRVAELFMFDFEISGIHLDKEKRKRAVDLNVKILDL-------------SSTFLMGTNFP 239

Query: 221 NNL--HHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGN 280
           N +  H L + I R+  S G              D   +  +   + DD VR+ AY    
Sbjct: 240 NKIEKHLLPEHIRRNFTSAG--------------DHIIIDGLHAESPDDLVREAAYKIFL 299

Query: 281 SSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSA 340
                 L  L++++++R  LA+++GY +++  A+   +A +P  V+ FL++LS+ +    
Sbjct: 300 YPNAGQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAKNPETVMQFLEKLSDKLSERT 359

Query: 341 DEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLK 400
            ++F  IR  K+K      E + PWD  YY+ ++++  YN+   +   +F L  C+EGL 
Sbjct: 360 LKDFEMIRGMKMKLNPQNSE-VMPWDPPYYSGVIRAERYNIEPSLYCPFFSLGACMEGLN 419

Query: 401 TLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFA 460
            L+N L G S  +   A GE W  DV KL++ H  EG LGY+Y D + R  K     HF 
Sbjct: 420 ILLNRLLGISLYAEQPAKGEVWSEDVRKLAVVHESEGLLGYIYCDFFQRADKPHQDCHFT 479

Query: 461 IQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 520
           I+GG    + +YQLPVV L+ N   S   S   L  S +E LFHE GHA+HS+L RT YQ
Sbjct: 480 IRGGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTPSMMENLFHEMGHAMHSMLGRTRYQ 539

Query: 521 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAAT 580
           H +GTR   D AE PS L EY+A+DYRV+  FA+HY TG+ +P+ +V  +  +KK+ AA 
Sbjct: 540 HVTGTRCPTDFAEVPSILMEYFANDYRVVNQFARHYQTGQPLPKNMVSRLCESKKVCAAA 599

Query: 581 ELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGA 640
           ++Q Q+ YA +DQ   G+       T+ ++ E + +     +V  T WQ RF HL+ YGA
Sbjct: 600 DMQLQVFYATLDQIYHGKH-PLRNSTTDILKETQEKFYGLPYVPNTAWQLRFSHLVGYGA 659

Query: 641 GYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIR 700
            YYSYL ++  A+ +W++   +DP +R+ G   R + L HGG +EP+     L+ +G+++
Sbjct: 660 RYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGREPM-----LMVEGMLQ 703

Query: 701 YCDGGVIPDITSLCKEMDI 715
            C   V   +++L  ++D+
Sbjct: 720 KCP-SVDDFVSALVSDLDL 703

BLAST of Carg08005 vs. ExPASy Swiss-Prot
Match: Q5RF14 (Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.6e-103
Identity = 226/679 (33.28%), Postives = 354/679 (52.14%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G   GL+G  +L +P+GF    ++A+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 60  GERRGLFGVPELSAPEGFHAAQEKALRKAELLVGRACSTPPGPQTVLIFDELSDSLCRVA 119

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHL---LTREA 160
           D A+  +  HP+  F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 120 DLADFVKIAHPEPAFREAAEEACRSIGTMVEKLNTNVDLYQSLRKLLADKKLVDSLDPET 179

Query: 161 HMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVP 220
              A     DFE +GIHL  E+  R   L+++I  L             S         P
Sbjct: 180 RRVAELFMFDFEISGIHLDKEKRKRAVDLNVKILDL-------------SSTFLMGANFP 239

Query: 221 NNL--HHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGN 280
           N +  H L + I R+  S G              D   +  +   + DD VR+ AY    
Sbjct: 240 NKIEKHLLPEHIRRNFTSAG--------------DHIIIDGLHAESPDDLVREAAYKIFL 299

Query: 281 SSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSA 340
                 L  L++++++R  LA+++GY +++  A+   +A +P  V+ FL++LS+ +    
Sbjct: 300 YPNAGQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAKNPETVMQFLEKLSDKLSERT 359

Query: 341 DEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLK 400
            ++F  IR  K+K      E + PWD  YY+ ++++  YN+   +   +F L  C+EGL 
Sbjct: 360 LKDFEMIRGMKMKLNPQNSE-VMPWDPPYYSGVIRAERYNIEPSLYCPFFSLGACMEGLN 419

Query: 401 TLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFA 460
            L+N L G S  +   A GE W  DV KL++ H  EG LGY+Y D + R  K     HF 
Sbjct: 420 ILLNRLLGISLYAEQPAKGEVWSEDVRKLAVVHESEGLLGYIYCDFFQRADKPHQDCHFT 479

Query: 461 IQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 520
           I+GG    + +YQLPVV L+ N   S   S   L    +E LFHE GHA+HS+L RT YQ
Sbjct: 480 IRGGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTPGMMENLFHEMGHAMHSMLGRTRYQ 539

Query: 521 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAAT 580
           H +GTR   D AE PS L EY+A+DYRV+  FA+HY TG+ +P+ +V  +  +KK+ AA 
Sbjct: 540 HVTGTRCPTDFAEVPSILMEYFANDYRVVNQFARHYQTGQPLPKNMVSRLCESKKVCAAA 599

Query: 581 ELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGA 640
           ++Q Q+ YA +DQ   G+       T+ ++ E + +     +V  T WQ RF HL+ YGA
Sbjct: 600 DMQLQVFYATLDQIYHGKH-PLRNSTTDILKETQEKFYGLPYVPDTAWQLRFSHLVGYGA 659

Query: 641 GYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIR 700
            YYSYL ++  A+ +W++   +DP +R+ G   R + L HGG +EP+     L+ +G+++
Sbjct: 660 KYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGREPM-----LMVEGMLQ 703

Query: 701 YCDGGVIPDITSLCKEMDI 715
            C   V   +++L  ++D+
Sbjct: 720 KCP-SVDDFVSALVSDLDL 703

BLAST of Carg08005 vs. ExPASy Swiss-Prot
Match: A6H611 (Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-98
Identity = 213/652 (32.67%), Postives = 335/652 (51.38%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G   GL+G  +L +P+GF+   +EA++++  LV    S P   + +   DE+S+ +C V 
Sbjct: 58  GERRGLFGVPELSTPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVA 117

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHL---LTREA 160
           D A+  +  HPD  F E A +A   +      LNTN  LY ++++   +  L   L  E 
Sbjct: 118 DLADFVKIGHPDPAFREAAQEACRSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAET 177

Query: 161 HMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVP 220
              A     DFE +GIHL  E+  R   L+++I  L   +                   P
Sbjct: 178 RRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSNAF-------------LMRTNFP 237

Query: 221 NNLHHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSS 280
           N +   + P +               G  L+ D      +    SDD VR+ AY      
Sbjct: 238 NKIRKSLLPEHIQ-------HHFARDGSHLIID-----GLHAEASDDLVREAAYKIFLYP 297

Query: 281 PCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADE 340
               L  L++++++R  LA+++GY +++  A+   +A +P  V+ FL++LS  +     +
Sbjct: 298 NADQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTRK 357

Query: 341 EFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTL 400
           +F  ++  K K    +   L PWD  YY+ ++++  YN+   +   +  L  C+EGL  L
Sbjct: 358 DFKMMQGMKTKLN-PQNSKLMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVL 417

Query: 401 VNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQ 460
            N L G +  +     GE W  D+ KL++ H  EG LGY+Y D + R  K     HF I+
Sbjct: 418 FNKLLGITLYAEQTFKGEVWCNDIRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIR 477

Query: 461 GGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHF 520
           GG    +  YQLPVV L+ N   +       L    +E LFHE GHA+HS+L RT YQH 
Sbjct: 478 GGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHV 537

Query: 521 SGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATEL 580
           +GTR   D AE PS L EY+++DYRV+  FAKHY TG+ +P+ +V  +  +KK+  A E+
Sbjct: 538 TGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCTAAEM 597

Query: 581 QRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGY 640
           Q Q+ YA +DQ   G+    ++ T+ ++ E + Q     +V  T WQ RF HL+ YGA Y
Sbjct: 598 QLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAKY 657

Query: 641 YSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLV 690
           YSYL ++  A+ IW++   +DP +R+ G   R + L HGG KEP+ +++ ++
Sbjct: 658 YSYLMSRAVASMIWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGML 682

BLAST of Carg08005 vs. ExPASy Swiss-Prot
Match: Q01992 (Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.4e-96
Identity = 215/658 (32.67%), Postives = 334/658 (50.76%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G   GL+G  +L +P+GF+   +EA+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 58  GERRGLFGVPELSTPEGFQVAQEEALRKTEWLVERACSTPPGPQTVLIFDELSDCLCRVA 117

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHL---LTREA 160
           D A+  +  HP++ F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 118 DLADFVKIGHPEQAFREAAQEACRSIGTMVEKLNTNVELYQSLQKLLDDKKLMDSLDAET 177

Query: 161 HMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVP 220
              A     DFE +GIHL  E+  R   L+++I  L   +               P+++ 
Sbjct: 178 RRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSSAFLMGTNF---------PIKIQ 237

Query: 221 NNL------HHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAY 280
            +L      HH  +                  G  L+ D      +    SDD VR+ AY
Sbjct: 238 KHLLPEHIQHHFAR-----------------DGRHLVID-----GLHAEASDDLVREAAY 297

Query: 281 VKGNSSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLV 340
                     L  L++++++R  LA ++GY  +        +A +P  V+ FL++LS  +
Sbjct: 298 KIFLYPNADQLKCLEELLSSRDLLANLVGYLPFPT-GPPGTIAQTPETVMQFLEKLSEKL 357

Query: 341 RPSADEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCI 400
                ++F  ++  K K      E L PWD  YY+ ++++  YN+   +   +  L  C+
Sbjct: 358 CERTRKDFEMMQGMKTKLNPQNSE-LMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACM 417

Query: 401 EGLKTLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGC 460
           EGL  L N L G +  +     GE W  DV KL++ H  EG LGY+Y D + R  K    
Sbjct: 418 EGLNVLFNRLLGVTLYAEQPFKGEVWCIDVRKLAVVHESEGLLGYIYCDFFQRANKPQQD 477

Query: 461 AHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSR 520
            HF I+GG    +  YQLPVV L+ N   +       L    +E LFHE GHA+HS+L R
Sbjct: 478 CHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGR 537

Query: 521 TEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKM 580
           T YQH +GTR   D AE PS L EY+++DYRV+  FAKHY TG+ +P+ +V  +  +KK+
Sbjct: 538 TRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKV 597

Query: 581 FAATELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLL 640
            AA E+Q Q+ YA +DQ   G+    ++ T+ ++ E + Q     +V  T WQ RF HL+
Sbjct: 598 CAAAEMQLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLV 657

Query: 641 TYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLV 690
            YGA YYSYL ++  A+ +W++   +DP +R+ G   R + L HGG KEP+ +++ ++
Sbjct: 658 GYGAKYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGML 681

BLAST of Carg08005 vs. ExPASy TrEMBL
Match: A0A6J1FLV9 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446997 PE=3 SV=1)

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 713/720 (99.03%), Postives = 716/720 (99.44%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCL SRSIHAAASAPHEG ATGLYGFEQLKSPKGFRR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQI+EFKLKKRFNKFEDLEPW
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIKEFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL+S VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHP EGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSS+VAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720

BLAST of Carg08005 vs. ExPASy TrEMBL
Match: A0A6J1I911 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471087 PE=3 SV=1)

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 707/720 (98.19%), Postives = 712/720 (98.89%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           MLSLIRKTTRKVSSGAVLKSCYLNCL SRSIHAAASAPHEG ATGLYGFEQLKSPKGF+R
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFQR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
           KAAMRMSEYFHFLNTNHTLYNAVKKAE EAHLLTREAHMAAHFLRVDFERAGIHLSAERL
Sbjct: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEHEAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM
Sbjct: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSP ANLDVLDKIIATRHELAQILG
Sbjct: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPRANLDVLDKIIATRHELAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW
Sbjct: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL+S VISSYFPL+QCIEGLKTLVNSLFGASFNSI LAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLNSSVISSYFPLTQCIEGLKTLVNSLFGASFNSISLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSLHHP EGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG
Sbjct: 421 VLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLT ERD
Sbjct: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           TSS+VAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL
Sbjct: 601 TSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720
           SRSTGTALRTKFLQHGGSKEPVDLLKDL+GDGIIRYCDGGVIPDITSLCKEMDISGKLVV
Sbjct: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKLVV 720

BLAST of Carg08005 vs. ExPASy TrEMBL
Match: A0A6J1CH32 (probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011592 PE=3 SV=1)

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 621/689 (90.13%), Postives = 650/689 (94.34%), Query Frame = 0

Query: 30  SIHAAASAPHEGPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAM 89
           SIHA ASAP +G ATGLYGFE LKSPKGFRRFVDEAIERSGELVTFISS PSSAEVIRAM
Sbjct: 1   SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM 60

Query: 90  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAERE 149
           DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMR++EYFH+LNTNHTLYNAVKKAE E
Sbjct: 61  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE 120

Query: 150 AHLLTREAHMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVD 209
           AHLLTREAH AAH+LRVDFERAGIHLSAERLDRVNQLSIEISQLCQE+KENIVIDPGSVD
Sbjct: 121 AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD 180

Query: 210 IFPPMRVPNNLHHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKM 269
           IFPP+ +PN LHHLVKPIYRSS SFGS   MKE GFRL+TDAD+L S+LQY SDDEVRKM
Sbjct: 181 IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM 240

Query: 270 AYVKGNSSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSN 329
           AY+KGNSSP ANLDVLDK+IATRHELAQI+GYRSYAEFAVTPN+ASSPAVV+SFLQELS 
Sbjct: 241 AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK 300

Query: 330 LVRPSADEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQ 389
           +VRP ADEEFNQIREFKLKK  NK EDLEPWDEAYYTSMMKSTAYNL S V++SYFPLSQ
Sbjct: 301 VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSVVASYFPLSQ 360

Query: 390 CIEGLKTLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYP 449
           CIEGLK LV SLFGASF SIPLAPGESWHPDVLKLSLHHP EGDLGYLYLDLYSRKGKYP
Sbjct: 361 CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP 420

Query: 450 GCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLL 509
           GCAHFAI+GG KVSEMEYQLPVVALVCNFS S  RSNVRLNHSEVETLFHEFGHALHSLL
Sbjct: 421 GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL 480

Query: 510 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAK 569
           SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTG++IPEKLVKSMQGAK
Sbjct: 481 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK 540

Query: 570 KMFAATELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFH 629
           KMF ATELQRQILYALIDQTLFGERLTSERDTSS+VAELKRQ T+WKHVD THWQTRF H
Sbjct: 541 KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH 600

Query: 630 LLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLV 689
           LLTYGAGYYSYLYAKCF+ATIWEKLCQEDPLSR+TGTALRTKFLQHGGSKE VDLL DLV
Sbjct: 601 LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 660

Query: 690 GDGIIRYCDGGVIPDITSLCKEMDISGKL 719
           GDGI+RYCDGGVIPD+TSLCKEMD+SG L
Sbjct: 661 GDGIVRYCDGGVIPDLTSLCKEMDLSGSL 689

BLAST of Carg08005 vs. ExPASy TrEMBL
Match: A0A0A0L676 (Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176300 PE=3 SV=1)

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 623/718 (86.77%), Postives = 667/718 (92.90%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           M SLIRKT  K++SG VLKS Y NC  SRSIHA ASAP +G ATGLYGF+ LKSP+GFRR
Sbjct: 1   MSSLIRKTPGKLTSGLVLKSSYWNCFRSRSIHAPASAPQKGAATGLYGFDHLKSPQGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVIRAMDEISN VCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNSVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
            AAMRM+EYFHFLNTNHTLY+AVKKAE EAHLLTREAHMAAH+LRVDFERAGIHLSA++L
Sbjct: 121 NAAMRMNEYFHFLNTNHTLYSAVKKAEHEAHLLTREAHMAAHYLRVDFERAGIHLSADKL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQL+IEISQLCQE+KENI+IDPG VDIFPP+R+PNNLHHL KPIYRSS+SFGS SS 
Sbjct: 181 DRVNQLNIEISQLCQEFKENILIDPGYVDIFPPLRMPNNLHHLAKPIYRSSESFGSRSSK 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           KE GFRLMTD+DSLSSILQ+ SDDEVRKMAYVKGNSSP ANL VLDK+IATRH LAQILG
Sbjct: 241 KENGFRLMTDSDSLSSILQFASDDEVRKMAYVKGNSSPRANLGVLDKLIATRHSLAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRS+AEFAVTPN+ASSPAVV+SFLQELS +VR  ADEEFNQIREFKLKK  NKFEDLEPW
Sbjct: 301 YRSFAEFAVTPNLASSPAVVMSFLQELSKVVRSRADEEFNQIREFKLKKCINKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL S VI+SYFPLSQCIEGLKTLV SLFGASF +IPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLDSSVIASYFPLSQCIEGLKTLVKSLFGASFYNIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSL HP EG+LG+LYLDLYSRKGKYPGCAHFAI+GG KVSE EYQLPVVALVCNFS 
Sbjct: 421 VLKLSLQHPEEGELGFLYLDLYSRKGKYPGCAHFAIRGGRKVSETEYQLPVVALVCNFSS 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           S +RSNVRLNHSE+ETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SNDRSNVRLNHSELETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVL+TFAKHYSTGE++PEKLVKSM+GAK MFAATELQRQILYALIDQTLFGE+LTSERD
Sbjct: 541 YRVLKTFAKHYSTGEILPEKLVKSMKGAKMMFAATELQRQILYALIDQTLFGEKLTSERD 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           T S+VA+LKRQ TSWKHVDGTHWQ++F HLLTYGAGYY+YLYAKCFAATIWEKLC+EDPL
Sbjct: 601 TVSVVADLKRQYTSWKHVDGTHWQSQFCHLLTYGAGYYTYLYAKCFAATIWEKLCKEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 719
           SR TG ALRTKFLQHGGSKE VDLL DLVGDGIIRY +GGVIPDITSLCKEM ++  L
Sbjct: 661 SRETGNALRTKFLQHGGSKEAVDLLTDLVGDGIIRYSEGGVIPDITSLCKEMGLTKNL 718

BLAST of Carg08005 vs. ExPASy TrEMBL
Match: A0A1S3AYL6 (probable mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483995 PE=3 SV=1)

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 618/718 (86.07%), Postives = 665/718 (92.62%), Query Frame = 0

Query: 1   MLSLIRKTTRKVSSGAVLKSCYLNCLGSRSIHAAASAPHEGPATGLYGFEQLKSPKGFRR 60
           M SL+RKTT K++SG VLKS Y NC   RSIHA ASAP +G ATGLYGF+ LKSP+GFRR
Sbjct: 1   MSSLVRKTTGKLTSGLVLKSSYWNCSRFRSIHAPASAPQKGAATGLYGFDHLKSPQGFRR 60

Query: 61  FVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEAN 120
           FVDEAIERSGELVTFISSMPSSAEVI+AMDEISN VCSVFDSAELCRQTHPDREFVEEAN
Sbjct: 61  FVDEAIERSGELVTFISSMPSSAEVIQAMDEISNSVCSVFDSAELCRQTHPDREFVEEAN 120

Query: 121 KAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERL 180
            AAMRM+EYFHFLNTNHTLY+AVKKAE E HLLTREAHMAAH+LRVDFERAGIHLSA++L
Sbjct: 121 NAAMRMNEYFHFLNTNHTLYSAVKKAEHETHLLTREAHMAAHYLRVDFERAGIHLSADKL 180

Query: 181 DRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSM 240
           DRVNQL+IEISQLCQE+KENIVIDPG VDIFPP+R+PNNL+HL KPIYRS++  GS SS 
Sbjct: 181 DRVNQLNIEISQLCQEFKENIVIDPGYVDIFPPLRMPNNLYHLAKPIYRSNELLGSRSSK 240

Query: 241 KEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQILG 300
           +E GFRLMTD+DSLSSILQ+TSDDEVRKMAYVKGNSSP ANL VLDK+IATRH LAQILG
Sbjct: 241 EENGFRLMTDSDSLSSILQFTSDDEVRKMAYVKGNSSPRANLGVLDKLIATRHSLAQILG 300

Query: 301 YRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPW 360
           YRS+AEFAVTPN+ASSPAVV+SFLQELS +VR  ADEEFNQIREFKLKK  NKFEDLEPW
Sbjct: 301 YRSFAEFAVTPNLASSPAVVMSFLQELSKVVRSRADEEFNQIREFKLKKCMNKFEDLEPW 360

Query: 361 DEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPD 420
           DEAYYTSMMKSTAYNL S  I+SYFPLSQCIEGLKTLV SLFGASF +IPLAPGESWHPD
Sbjct: 361 DEAYYTSMMKSTAYNLDSSAIASYFPLSQCIEGLKTLVKSLFGASFYNIPLAPGESWHPD 420

Query: 421 VLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSG 480
           VLKLSL HP EG+LG+LYLDLYSRKGKYPGCAHFAI+GG KVSE EYQLPVVALVCNFS 
Sbjct: 421 VLKLSLQHPEEGELGFLYLDLYSRKGKYPGCAHFAIKGGRKVSETEYQLPVVALVCNFSS 480

Query: 481 SRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540
           S +RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD
Sbjct: 481 SNDRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASD 540

Query: 541 YRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERD 600
           YRVL+TFAKHYSTGE+IPEKLVKSMQGAK MFAATELQRQILYALIDQTLFGE+LTSER 
Sbjct: 541 YRVLKTFAKHYSTGEIIPEKLVKSMQGAKMMFAATELQRQILYALIDQTLFGEKLTSERG 600

Query: 601 TSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPL 660
           T+S+VA+LKRQ TSWKHVDGTHWQ++F HLLTYGAGYY+YLYAKCFAATIWEKLC+EDPL
Sbjct: 601 TASVVADLKRQYTSWKHVDGTHWQSQFCHLLTYGAGYYTYLYAKCFAATIWEKLCKEDPL 660

Query: 661 SRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 719
           SR TG ALRTKFL+HGGSKEPVDLL DLVGDGIIRY +GGVIPDITSLCKEM ++  L
Sbjct: 661 SRETGNALRTKFLEHGGSKEPVDLLTDLVGDGIIRYREGGVIPDITSLCKEMGLTKNL 718

BLAST of Carg08005 vs. TAIR 10
Match: AT5G51540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 941.4 bits (2432), Expect = 4.2e-274
Identity = 461/677 (68.09%), Postives = 547/677 (80.80%), Query Frame = 0

Query: 41  GPATGLYGFEQLKSPKGFRRFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVF 100
           G ATGLYGF+ LK+ KGF+RFV +AIERSGELV++IS MPSS E+I+AMDEIS+ VC V 
Sbjct: 28  GDATGLYGFDHLKTAKGFQRFVADAIERSGELVSYISGMPSSPEIIKAMDEISDTVCCVV 87

Query: 101 DSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMA 160
           DSAELCRQTHPDREFVEEANKAA+ M++Y H LNTNHTLY AVKKAE++++LLT+EA   
Sbjct: 88  DSAELCRQTHPDREFVEEANKAAIEMNDYLHHLNTNHTLYAAVKKAEQDSNLLTKEASRT 147

Query: 161 AHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNL 220
           AH LR+DFER GIHL  E+LD+VN L+  I QLC+E+ ENI  DPG VDIFP  R+P +L
Sbjct: 148 AHHLRMDFERGGIHLDPEKLDKVNNLTTNIFQLCREFSENIADDPGHVDIFPGSRIPRHL 207

Query: 221 HHLVKPIYRSSK-----SFGSISSMKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGN 280
           HHL+ P YRS+      S  S    K+KGFR+ TD  ++SSILQ+TSD+EVRKM Y++GN
Sbjct: 208 HHLLNPTYRSTSGGSRGSTRSAHKSKQKGFRINTDPRTVSSILQWTSDEEVRKMVYIQGN 267

Query: 281 SSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSA 340
           S P AN  VL+K+IA RHEL+Q++G  SYA+  V PN+A SP VV SFLQELS  V+P A
Sbjct: 268 SVPHANHGVLEKLIAARHELSQMMGCNSYADIMVEPNLAKSPKVVTSFLQELSKTVKPKA 327

Query: 341 DEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLK 400
           DEEF  IR+FK +K  N   +LEPWDE YYTSMMKS+  ++ + V++SYFPL QCIEGLK
Sbjct: 328 DEEFIAIRDFKREKCGNPSAELEPWDETYYTSMMKSSINDVDTAVVASYFPLPQCIEGLK 387

Query: 401 TLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHFA 460
            LV SLFGA+F++IPLAPGESWHP+V+KLSLHHP EGDLGYLYLDLYSRKGKYPGCA FA
Sbjct: 388 VLVESLFGATFHTIPLAPGESWHPNVVKLSLHHPDEGDLGYLYLDLYSRKGKYPGCASFA 447

Query: 461 IQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 520
           I+GG K+SE EYQLPV+ALVCNFS + + S V+LNHSEVE LFHEFGHALHSLLSRT+YQ
Sbjct: 448 IRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNHSEVEVLFHEFGHALHSLLSRTDYQ 507

Query: 521 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAAT 580
           HFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HYSTGE IPEKLV S+QGA+ MFAAT
Sbjct: 508 HFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHYSTGETIPEKLVNSLQGARNMFAAT 567

Query: 581 ELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFFHLLTYGA 640
           E+QRQ+ YALIDQ LFGE+  + RD S LVAELKRQ TSW HV+GTHW  RF HLL YGA
Sbjct: 568 EMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQHTSWNHVEGTHWYIRFSHLLNYGA 627

Query: 641 GYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIR 700
           GYYSYLYAKCFA+TIW+ +C+EDPLS +TGT LR KF +HGG+K+P +LL DL G  II 
Sbjct: 628 GYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREKFFKHGGAKDPAELLTDLAGKEIIS 687

Query: 701 YCDGGVIPDITSLCKEM 713
               G++P  T L  E+
Sbjct: 688 VHGEGIVPATTYLLNEL 704

BLAST of Carg08005 vs. TAIR 10
Match: AT1G67690.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-49
Identity = 152/615 (24.72%), Postives = 281/615 (45.69%), Query Frame = 0

Query: 81  SSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTNHTLY 140
           S   V+  + E+  R  S+       +   P     + + +A  ++  +         +Y
Sbjct: 97  SYENVVLPLAELEARQLSLIQCCVFPKMLSPHDNVRKASTEAEQKIDAHILSCRKREDVY 156

Query: 141 NAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEYKEN 200
             +K    +   ++ EA      L  DFE  G++L+A + + V +L  EI +L   Y +N
Sbjct: 157 RIIKIYAAKGESISPEAKCYLQCLVRDFEDNGLNLTAIKREEVERLKYEIDELSLRYIQN 216

Query: 201 IVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSMKEKGFRLMTDADSLSSILQY 260
           +  D   +           L  L            ++   + K F+L  ++  +++IL+ 
Sbjct: 217 LNEDSSCLFFTEDELAGLPLEFL-----------QNLEKTQNKEFKLTLESRHVAAILEL 276

Query: 261 TSDDEVRK-MAYVKGNSSPCANLDVLDKIIATRHELAQILGYRSYAEFAVTPNMASSPAV 320
               + RK +A   G      N+ VL +++ +RH LA + GY  +A++A+   M+ +   
Sbjct: 277 CKIAKTRKTVAMAYGKRCGDTNIPVLQRLVQSRHRLACVCGYAHFADYALDRRMSKTSMR 336

Query: 321 VVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEPWDEAYYTSMMKSTAYNLSSP 380
           V+ FL+++S+ +   A  EF+ + + K K+       +E  D  YY   ++   ++L   
Sbjct: 337 VIRFLEDISSSLTDLAIREFSILEDLKRKEEGEIPFGVE--DLLYYIKRVEELQFDLDFG 396

Query: 381 VISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHPDVLKLSLHHPGEGD-LGYLY 440
            I  YFP++  + G+  +   LFG  F  +     + W+ D+   ++   G G  LGY Y
Sbjct: 397 DIRQYFPVNLVLSGIFKICQDLFGIKFEEV--TEVDVWYHDIRAFAVFDSGSGKLLGYFY 456

Query: 441 LDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNHSEVETLF 500
           LD+++R+GK       A+Q     S    Q+PV  L+  F+       V L  S+V  LF
Sbjct: 457 LDMFTREGKCNHSCVVALQNNALFSNGACQIPVALLIAQFAKDGSGEAVPLGFSDVVNLF 516

Query: 501 HEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKH-YSTGEVI 560
           HEFGH +  + +R  +  FSG RV  D  E PS L E +  +   L+  + +     + +
Sbjct: 517 HEFGHVVQHICNRASFARFSGLRVDPDFREIPSQLLENWCYESFTLKLISGYRQDITKPL 576

Query: 561 PEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSLVAEL-KRQCTSWK 620
            +++ K+++  +  F+A +  ++ILY L DQ ++ +    + D   L+  L  +      
Sbjct: 577 VDEVCKTLKRWRYSFSALKSLQEILYCLFDQIIYSD---DDADLLQLIRSLHPKVMIGLP 636

Query: 621 HVDGTHWQTRFFH-LLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTKFLQH 680
            V+GT+  + F   ++   A  YS L+++ +AA I+     +   +   G   R K L  
Sbjct: 637 VVEGTNPASCFPRAVIGSEATCYSRLWSEVYAADIFASKFGDGHPNLYAGLQFRDKVLAP 693

Query: 681 GGSKEPVDLLKDLVG 691
           GG KEP++LL + +G
Sbjct: 697 GGGKEPMELLTNFLG 693

BLAST of Carg08005 vs. TAIR 10
Match: AT5G65620.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 164.9 bits (416), Expect = 2.5e-40
Identity = 153/647 (23.65%), Postives = 286/647 (44.20%), Query Frame = 0

Query: 80  PSSAEVIRAMDEISNR---VCSVFDSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTN 139
           P+  +++  +++I +R   V  + +  +  + T   R  +E+     ++       L  +
Sbjct: 141 PTWPKLVEPLEKIVDRLTVVWGMINHLKAVKDTPELRAAIEDVQPEKVKFQLR---LGQS 200

Query: 140 HTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERA---GIHLSAERLDRVNQLSIEISQL 199
             +YNA  KA RE+   +  +      +    + A   GI L  E+ +  N++  E+ +L
Sbjct: 201 KPIYNAF-KAIRESPDWSSLSEARQRLVEAQIKEAVLIGIALDDEKREEFNKIEQELEKL 260

Query: 200 CQEYKENI----------VIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSMKEK 259
             ++ EN+          + D   ++  PP          +    +++ S G  ++  E 
Sbjct: 261 SHKFSENVLDATKKFEKLITDKKEIEGLPP--------SALGLFAQAAVSKGHENATAEN 320

Query: 260 GFRLMT-DADSLSSILQYTSDDEVRKMAY----VKGNSSPCANLDVLDKIIATRHELAQI 319
           G  ++T DA S   ++Q+  +  +R+  Y     + +S    N  ++D+I+  R E A++
Sbjct: 321 GPWIITLDAPSYLPVMQHAKNRALREEVYRAYLSRASSGDLDNTAIIDQILKLRLEKAKL 380

Query: 320 LGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLE 379
           LGY +YAE ++   MA+        L++L +    +A ++   ++ F   +   + + + 
Sbjct: 381 LGYNNYAEVSMAMKMATVEK-AAELLEKLRSASWDAAVQDMEDLKSFAKNQGAAESDSMT 440

Query: 380 PWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIP-LAPGESW 439
            WD  +++  ++ + Y+++   +  YF L + ++GL +L  +LFG        LAP   W
Sbjct: 441 HWDTTFWSERLRESKYDINEEELRPYFSLPKVMDGLFSLAKTLFGIDIEPADGLAP--VW 500

Query: 440 HPDVLKLSLHHPGEGDLGYLYLDLYSRKGKYPGCAHF-AIQGGCKVSEME---YQLPVVA 499
           + DV    +       + Y Y D YSR  +  G A    +    +V   +    +LPV  
Sbjct: 501 NNDVRFYRVKDSSGNPIAYFYFDPYSRPSEKRGGAWMDEVVSRSRVMAQKGSSVRLPVAH 560

Query: 500 LVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAETPSN 559
           +VCN +         +   EVET+FHEFGHAL  +L++ +    +G R +  D  E PS 
Sbjct: 561 MVCNQTPPVGDKPSLMTFREVETVFHEFGHALQHMLTKQDEGLVAGIRNIEWDAVELPSQ 620

Query: 560 LFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFG 619
             E +      L + AKHY TGE +PE++ K +  A+   A +   RQ+ +A +D  L  
Sbjct: 621 FMENWCYHRDTLMSIAKHYETGETLPEEVYKKLLAARTFRAGSFSLRQLKFASVDLELHT 680

Query: 620 ERLTSERDTSSLV---AELKRQCTSWKHVDGTHWQTRFFHLLT--YGAGYYSYLYAKCFA 679
           + +    ++   V     +K Q       D   +   F H+    Y AGYYSY +A+  +
Sbjct: 681 KYVPGGPESIYDVDQRVSVKTQVIPPLPED--RFLCSFSHIFAGGYAAGYYSYKWAEVLS 740

Query: 680 ATIWEKL----CQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVG 691
           A  +         +    + TG   R   L  GG K P+ +  +  G
Sbjct: 741 ADAFSAFEDAGLDDIKAVKETGQRFRNTILALGGGKAPLKVFVEFRG 770

BLAST of Carg08005 vs. TAIR 10
Match: AT5G10540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 162.5 bits (410), Expect = 1.2e-39
Identity = 154/651 (23.66%), Postives = 284/651 (43.63%), Query Frame = 0

Query: 80  PSSAEVIRAMDEISNR---VCSVFDSAELCRQTHPDREFVEEANKAAMRMSEYFHFLNTN 139
           PS  +++  +++I +R   V  + +  +  + T   R  +EE     ++       L  +
Sbjct: 53  PSWPKLVEPLEKIIDRLSVVWGMINHLKAVKDTPELRAAIEEVQPEKVKFQLR---LGQS 112

Query: 140 HTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERA---GIHLSAERLDRVNQLSIEISQL 199
             +YNA  KA RE+      +      +    + A   GI L  ++ +  N++  E+ +L
Sbjct: 113 KPIYNAF-KAIRESPDWNSLSEARQRLVEAQIKEAVLSGIALEDDKREEFNKIEQELEKL 172

Query: 200 CQEYKENI----------VIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISSMKEK 259
             ++ EN+          + D   ++  PP          +    +++ S G  ++  + 
Sbjct: 173 SHKFSENVLDATKKFEKLITDKKEIEGLPP--------SALGLFAQAAVSKGHETATADT 232

Query: 260 GFRLMT-DADSLSSILQYTSDDEVRKMAY----VKGNSSPCANLDVLDKIIATRHELAQI 319
           G  L+T DA S   ++Q+  +  +R+  Y     + +S    N  ++D+I+  R E A++
Sbjct: 233 GPWLITLDAPSYLPVMQHAKNRALREEVYRAYLSRASSGDLDNTAIIDQILKLRLEKAKL 292

Query: 320 LGYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLE 379
           LGYR+YAE ++   MA+        L++L +     A ++   ++ F   +   + + L 
Sbjct: 293 LGYRNYAEVSMATKMATVEK-ADELLEKLRSASWDPAVQDIEDLKSFAKNQGAAEADSLT 352

Query: 380 PWDEAYYTSMMKSTAYNLSSPVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGES-- 439
            WD  +++  ++ + Y+++   +  YF L + ++ L  L  +LFG     I + P +   
Sbjct: 353 HWDITFWSERLRESKYDINEEELRPYFSLPKVMDALFGLAKTLFG-----IDVVPADGVA 412

Query: 440 --WHPDVLKLSLHHPGEGDLGYLYLDLYSRKG-KYPGCAHFAIQGGCKVSEME---YQLP 499
             W+ DV    +         Y Y D YSR   K  G     +    +V   +    +LP
Sbjct: 413 PVWNSDVRFYCVKDSSGNPTAYFYFDPYSRPSEKRDGAWMDEVFSRSRVMAQKGSSVRLP 472

Query: 500 VVALVCNFSGSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAET 559
           V  +VCN +         +   EVET+FHEFGHAL  +L++ +    +G R +  D  E 
Sbjct: 473 VAQMVCNQTPPVGDKPSLMTFREVETVFHEFGHALQHMLTKEDEGLVAGIRNIEWDAVEL 532

Query: 560 PSNLFEYYASDYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQT 619
           PS   E +      L + AKHY TGE +PE + K +  A+   A +   RQ+ +A +D  
Sbjct: 533 PSQFMENWCYHRDTLMSIAKHYQTGETLPENVYKKLLAARTFRAGSLSLRQLKFATVDLE 592

Query: 620 LFGERLTSERDTSSLVAELKRQCTSWKHVDGTHWQTRFF----HLLT--YGAGYYSYLYA 679
           L  + +    +T   + E+ ++ +    V     + RF     H+    Y AGYYSY +A
Sbjct: 593 LHTKYMPGGAET---IYEVDQRVSIKTQVIPPLPEDRFLCSFSHIFAGGYAAGYYSYKWA 652

Query: 680 KCFAATIWEKL----CQEDPLSRSTGTALRTKFLQHGGSKEPVDLLKDLVG 691
           +  +A  +         +    + TG   R   L  GG K P+ +  +  G
Sbjct: 653 EVLSADAFSAFEDAGLDDIKAVKETGQRFRNTILALGGGKAPLKVFVEFRG 682

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030829.10.0e+00100.00putative mitochondrial intermediate peptidase, mitochondrial [Cucurbita argyrosp... [more]
XP_022941716.10.0e+0099.03mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moscha... [more]
XP_023518581.10.0e+0098.33mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo s... [more]
XP_022972575.10.0e+0098.19mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita maxima... [more]
KAG6600162.10.0e+0094.17Mitochondrial intermediate peptidase, mitochondrial, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
F4KDA55.9e-27368.09Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q997971.2e-10333.43Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2[more]
Q5RF142.6e-10333.28Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1[more]
A6H6111.1e-9832.67Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1[more]
Q019922.4e-9632.67Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1FLV90.0e+0099.03mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita mosc... [more]
A0A6J1I9110.0e+0098.19mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxi... [more]
A0A6J1CH320.0e+0090.13probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charan... [more]
A0A0A0L6760.0e+0086.77Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G17630... [more]
A0A1S3AYL60.0e+0086.07probable mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucum... [more]
Match NameE-valueIdentityDescription
AT5G51540.14.2e-27468.09Zincin-like metalloproteases family protein [more]
AT1G67690.11.7e-4924.72Zincin-like metalloproteases family protein [more]
AT5G65620.12.5e-4023.65Zincin-like metalloproteases family protein [more]
AT5G10540.11.2e-3923.66Zincin-like metalloproteases family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 136..156
NoneNo IPR availablePANTHERPTHR11804:SF79MITOCHONDRIAL INTERMEDIATE PEPTIDASE, MITOCHONDRIALcoord: 32..713
NoneNo IPR availableCDDcd06457M3A_MIPcoord: 45..694
e-value: 0.0
score: 760.169
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 46..690
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 386..537
e-value: 1.9E-142
score: 477.4
IPR024077Neurolysin/Thimet oligopeptidase, domain 2GENE3D1.10.1370.10Neurolysin, domain 3coord: 181..690
e-value: 1.9E-142
score: 477.4
IPR001567Peptidase M3A/M3B catalytic domainPFAMPF01432Peptidase_M3coord: 258..691
e-value: 3.9E-121
score: 405.5
IPR045090Peptidase M3A/M3BPANTHERPTHR11804PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATEDcoord: 32..713

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08005-RACarg08005-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006518 peptide metabolic process
biological_process GO:0006508 proteolysis
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008237 metallopeptidase activity