Carg07943 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07943
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionnitrate regulatory gene2 protein-like isoform X2
LocationCarg_Chr07: 1822220 .. 1824664 (-)
RNA-Seq ExpressionCarg07943
SyntenyCarg07943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGCTGTTACTCGAGATTAGAGAGAGGAGAAACTGTGTCGAGGTGCAAAGCAAGAGAGAGGTACATGAAGCAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCCCTTCGCGGCACCGGCGCGGCTCTCCGGCAGTTTTCCAATGAAGAGACCTACATTCACCGCCCTCTGCACCACCACCTTCCGCCGGAGGTTCCATCTCCAACCCCACGAACGCCGCCACCGCCGCCTACACCTCCACCTCCACCTATCCCCATGAGTCCCAGTTCCGACACCTGGACGTCGACCACGGCCTCCACAGCTCTGCCTCCTCCTCCGCCACCACCGCCATCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACGTCTCGCACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTAGGATCACTGTAACAGGGGCGGCGAGTTCAGCGCCGCCGCCGTCGGTGGTGAGTGGTTTTTCAAAGGACGCTTCGAGCACTGAGCTTGCTGTGGTAGTGTCAAGGAACACTAAAGATCTGGTGGAGATTATCAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCCGAAGCTGGTGCTGGGCTCTCTGTGCTTTTGGAAGTTCCCACTTTCTCTTCTCAGAATAAAGGAGGTAAGTCTAATATCAAAGAACTAGAAAAGCAACCAAATCATACAGTAACCTAATGAGTTTCATATCATGTCGCTAGCAGGACAGGTTTACAGTAATAGGTGGAGCTTGAGCCCTTCGTTGAGGGTGTGGGGTTCAAACACAAAACCAGATGCATTAGGAAGATTAAACGGACAACTAACAGCAACAAATATGGGAAATGGATATACAGGGAATAGCGGTAGCCACTGCTCAACAGTGGAGAAGCTGTATGCTTGGGAGAAAAAACTGTACCAGGAGGTCAAGGTAATGCTATCACTTTCGCGTATTTCAATCAAATCTCCAGTCAGATTGGAGGAATAATATGACATTTTGGTGCTTTAAATAATAAAATCTAATTTGAAATGATTCTTCCCACTTTTTCTTCCTTTTACCTAATGGGGTCCAACATAACAAAACAAAGATGAAACCTTTAGCATTTCTCAATGCAATTGAAGGCAGTGAAGAACCGAATTGTGGTATCCTTTGTACTATAGTTTGGTGAAATTATTGTTTGAGTGGCTTTTTTAGATTGCCGAGGCCATAAGGATAGAGCATGAGAAGAAGGTGGACCTATTGAGAAAGCTAGAACTGAGGAGGGCTGATTACCTGAAGACTGAGAAGACCAAGAAAGAGGTCGAGAAGTTGGAGTCCCAGATGATGGTTGCTTCTCAGGCCATCGAAACCACATCGACTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGTTTGTCAAAGGGTTAGTCTCTTTCAAATTTCTGTTTTGGCCTCAACAGAAATAGGAAATCGCCAGTCATTTGACTGTACTCTCAATGTATTGTTTTGCGACCTCTGGTGTTTCAGGAAGCCTAACTGAAAAATTACGAAGCATCTTTTGGATGTCTTCATGGGCATAAATAGCATTGCATCTGAACTTGGAAGTATACTAAACACTAAGACTTTCCTTGGTTTCTACAGATTGATGTGCATGTGGAGGAGTATGTACGAGTGTCATCAAGTACAAACACACATTGTTGAGCAGCTCAAATACCTAAACATCTTACCATCTGCTGAGCCAACATCTGAGATCCACCGCCAATCCACTCTCCAGCTTGAGCTTGAAGTTCAGCAATGGCACGGTTCTTTTTGCAATTTAGTCAAAGCTCAACGGGATTATGTCCAATCTCTTACAGGCTGGCTCCGGCTTAGCCTTTTCCAGATAAGTGGAAACCCCTTATTAAGAACAGATCAACATTCTAGAATTTACGAACTTTGTGAAGAATGGAACCTTGCAGTTGATAGGATTCCGGACAAAGTGGCTTCCGAAGGGATCAAGAGCTTCTTAACCGTAATCCATGCCATTGTTGTTCAACAAGCTGCGGAAAACAGGCAAAGGAAGAAGGCAGATTCTGCCTCGAAAGAGCTTGATAAGAGAGCTAACGAGCTAAGATCGCTAGAAAGTAAATATGTCTCACATTCAATGCGAGAATGCTCAGCATCCACGAGAGGGAGAGACCCAGTTCAGGAGAAGAAAAATAAGGTGGAGAACTTGAGAACAAAAGCTCGAGATGAGAAGCAAAAGTATGAAAGTTTGATCAGTGTGACAAGGTCAATGACAATATACAACTTGCAGATGAGTTTTCCCCATGTTTTCCAAGCAATGGTCGGGTTTTCAAGTGTATGCATGCACGCATATGAGGCAGTTTACAATCAAACTAAAAATTCTAACCAGCATGAGGTGAAGAGATTACTACCGTGA

mRNA sequence

ATGGGTAGCTGTTACTCGAGATTAGAGAGAGGAGAAACTGTGTCGAGGTGCAAAGCAAGAGAGAGGTACATGAAGCAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCCCTTCGCGGCACCGGCGCGGCTCTCCGGCAGTTTTCCAATGAAGAGACCTACATTCACCGCCCTCTGCACCACCACCTTCCGCCGGAGGTTCCATCTCCAACCCCACGAACGCCGCCACCGCCGCCTACACCTCCACCTCCACCTATCCCCATGAGTCCCAGTTCCGACACCTGGACGTCGACCACGGCCTCCACAGCTCTGCCTCCTCCTCCGCCACCACCGCCATCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACGTCTCGCACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTAGGATCACTGTAACAGGGGCGGCGAGTTCAGCGCCGCCGCCGTCGGTGGTGAGTGGTTTTTCAAAGGACGCTTCGAGCACTGAGCTTGCTGTGGTAGTGTCAAGGAACACTAAAGATCTGGTGGAGATTATCAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCCGAAGCTGGTGCTGGGCTCTCTGTGCTTTTGGAAGTTCCCACTTTCTCTTCTCAGAATAAAGGAGGACAGGTTTACAGTAATAGGTGGAGCTTGAGCCCTTCGTTGAGGGTGTGGGGTTCAAACACAAAACCAGATGCATTAGGAAGATTAAACGGACAACTAACAGCAACAAATATGGGAAATGGATATACAGGGAATAGCGGTAGCCACTGCTCAACAGTGGAGAAGCTGTATGCTTGGGAGAAAAAACTGTACCAGGAGGTCAAGATTGCCGAGGCCATAAGGATAGAGCATGAGAAGAAGGTGGACCTATTGAGAAAGCTAGAACTGAGGAGGGCTGATTACCTGAAGACTGAGAAGACCAAGAAAGAGGTCGAGAAGTTGGAGTCCCAGATGATGGTTGCTTCTCAGGCCATCGAAACCACATCGACTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGTTTGTCAAAGGATTGATGTGCATGTGGAGGAGTATGTACGAGTGTCATCAAGTACAAACACACATTGTTGAGCAGCTCAAATACCTAAACATCTTACCATCTGCTGAGCCAACATCTGAGATCCACCGCCAATCCACTCTCCAGCTTGAGCTTGAAGTTCAGCAATGGCACGGTTCTTTTTGCAATTTAGTCAAAGCTCAACGGGATTATGTCCAATCTCTTACAGGCTGGCTCCGGCTTAGCCTTTTCCAGATAAGTGGAAACCCCTTATTAAGAACAGATCAACATTCTAGAATTTACGAACTTTGTGAAGAATGGAACCTTGCAGTTGATAGGATTCCGGACAAAGTGGCTTCCGAAGGGATCAAGAGCTTCTTAACCGTAATCCATGCCATTGTTGTTCAACAAGCTGCGGAAAACAGGCAAAGGAAGAAGGCAGATTCTGCCTCGAAAGAGCTTGATAAGAGAGCTAACGAGCTAAGATCGCTAGAAAGTAAATATGTCTCACATTCAATGCGAGAATGCTCAGCATCCACGAGAGGGAGAGACCCAGTTCAGGAGAAGAAAAATAAGGTGGAGAACTTGAGAACAAAAGCTCGAGATGAGAAGCAAAAGTATGAAAGTTTGATCAGTGTGACAAGGTCAATGACAATATACAACTTGCAGATGAGTTTTCCCCATGTTTTCCAAGCAATGGTCGGGTTTTCAAGTGTATGCATGCACGCATATGAGGCAGTTTACAATCAAACTAAAAATTCTAACCAGCATGAGGTGAAGAGATTACTACCGTGA

Coding sequence (CDS)

ATGGGTAGCTGTTACTCGAGATTAGAGAGAGGAGAAACTGTGTCGAGGTGCAAAGCAAGAGAGAGGTACATGAAGCAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCCCTTCGCGGCACCGGCGCGGCTCTCCGGCAGTTTTCCAATGAAGAGACCTACATTCACCGCCCTCTGCACCACCACCTTCCGCCGGAGGTTCCATCTCCAACCCCACGAACGCCGCCACCGCCGCCTACACCTCCACCTCCACCTATCCCCATGAGTCCCAGTTCCGACACCTGGACGTCGACCACGGCCTCCACAGCTCTGCCTCCTCCTCCGCCACCACCGCCATCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACGTCTCGCACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTAGGATCACTGTAACAGGGGCGGCGAGTTCAGCGCCGCCGCCGTCGGTGGTGAGTGGTTTTTCAAAGGACGCTTCGAGCACTGAGCTTGCTGTGGTAGTGTCAAGGAACACTAAAGATCTGGTGGAGATTATCAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCCGAAGCTGGTGCTGGGCTCTCTGTGCTTTTGGAAGTTCCCACTTTCTCTTCTCAGAATAAAGGAGGACAGGTTTACAGTAATAGGTGGAGCTTGAGCCCTTCGTTGAGGGTGTGGGGTTCAAACACAAAACCAGATGCATTAGGAAGATTAAACGGACAACTAACAGCAACAAATATGGGAAATGGATATACAGGGAATAGCGGTAGCCACTGCTCAACAGTGGAGAAGCTGTATGCTTGGGAGAAAAAACTGTACCAGGAGGTCAAGATTGCCGAGGCCATAAGGATAGAGCATGAGAAGAAGGTGGACCTATTGAGAAAGCTAGAACTGAGGAGGGCTGATTACCTGAAGACTGAGAAGACCAAGAAAGAGGTCGAGAAGTTGGAGTCCCAGATGATGGTTGCTTCTCAGGCCATCGAAACCACATCGACTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGTTTGTCAAAGGATTGATGTGCATGTGGAGGAGTATGTACGAGTGTCATCAAGTACAAACACACATTGTTGAGCAGCTCAAATACCTAAACATCTTACCATCTGCTGAGCCAACATCTGAGATCCACCGCCAATCCACTCTCCAGCTTGAGCTTGAAGTTCAGCAATGGCACGGTTCTTTTTGCAATTTAGTCAAAGCTCAACGGGATTATGTCCAATCTCTTACAGGCTGGCTCCGGCTTAGCCTTTTCCAGATAAGTGGAAACCCCTTATTAAGAACAGATCAACATTCTAGAATTTACGAACTTTGTGAAGAATGGAACCTTGCAGTTGATAGGATTCCGGACAAAGTGGCTTCCGAAGGGATCAAGAGCTTCTTAACCGTAATCCATGCCATTGTTGTTCAACAAGCTGCGGAAAACAGGCAAAGGAAGAAGGCAGATTCTGCCTCGAAAGAGCTTGATAAGAGAGCTAACGAGCTAAGATCGCTAGAAAGTAAATATGTCTCACATTCAATGCGAGAATGCTCAGCATCCACGAGAGGGAGAGACCCAGTTCAGGAGAAGAAAAATAAGGTGGAGAACTTGAGAACAAAAGCTCGAGATGAGAAGCAAAAGTATGAAAGTTTGATCAGTGTGACAAGGTCAATGACAATATACAACTTGCAGATGAGTTTTCCCCATGTTTTCCAAGCAATGGTCGGGTTTTCAAGTGTATGCATGCACGCATATGAGGCAGTTTACAATCAAACTAAAAATTCTAACCAGCATGAGGTGAAGAGATTACTACCGTGA

Protein sequence

MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEETYIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPSSTWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLRVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKNSNQHEVKRLLP
Homology
BLAST of Carg07943 vs. NCBI nr
Match: KAG6594763.1 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026728.1 hypothetical protein SDJN02_10733, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 627/627 (100.00%), Postives = 627/627 (100.00%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK
Sbjct: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKNSNQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 627

BLAST of Carg07943 vs. NCBI nr
Match: XP_022926423.1 (nitrate regulatory gene2 protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 624/627 (99.52%), Postives = 625/627 (99.68%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNLVKAQ+DYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK
Sbjct: 421 HGSFCNLVKAQQDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKAD ASKELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADYASKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNPNQHEVKRLLP 627

BLAST of Carg07943 vs. NCBI nr
Match: XP_022926422.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 624/628 (99.36%), Postives = 625/628 (99.52%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKG-GQVYSNRWSLSPSL 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKG GQVYSNRWSLSPSL
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGAGQVYSNRWSLSPSL 240

Query: 241 RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300
           RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR
Sbjct: 241 RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300

Query: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360
           IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY
Sbjct: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360

Query: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420
           PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ
Sbjct: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420

Query: 421 WHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD 480
           WHGSFCNLVKAQ+DYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD
Sbjct: 421 WHGSFCNLVKAQQDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD 480

Query: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMREC 540
           KVASEGIKSFLTVIHAIVVQQAAENRQRKKAD ASKELDKRANELRSLESKYVSHSMREC
Sbjct: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADYASKELDKRANELRSLESKYVSHSMREC 540

Query: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600
           SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF
Sbjct: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600

Query: 601 SSVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SSVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SSVCMHAYEAVYNQTKNPNQHEVKRLLP 628

BLAST of Carg07943 vs. NCBI nr
Match: XP_023518366.1 (nitrate regulatory gene2 protein-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 616/627 (98.25%), Postives = 619/627 (98.72%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE 
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEEI 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIH PLHHHLPPE+PSPTPRTPPPPP PPPPPIPMSPSSDTWTSTTASTALPPPPPPP S
Sbjct: 61  YIHPPLHHHLPPEIPSPTPRTPPPPPPPPPPPIPMSPSSDTWTSTTASTALPPPPPPPLS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDA G+LNGQL ATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDAFGKLNGQLIATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIP+K
Sbjct: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPEK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKADSA KELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSALKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNPNQHEVKRLLP 627

BLAST of Carg07943 vs. NCBI nr
Match: XP_023003866.1 (nitrate regulatory gene2 protein-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 613/627 (97.77%), Postives = 620/627 (98.88%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLE+GETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE+
Sbjct: 1   MGSCYSRLEKGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEES 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           Y+H PLHHHLPPEVPSPTPRTPPPPPT PPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YVHPPLHHHLPPEVPSPTPRTPPPPPTLPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEA AGLSVLLE+PTFSSQNKGGQVYSNRWSLSP LR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEASAGLSVLLEIPTFSSQNKGGQVYSNRWSLSPKLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDA G+LNGQLTATNMG GYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDAFGKLNGQLTATNMGTGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNL+KAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIY+LCEEWNLAVDRIPDK
Sbjct: 421 HGSFCNLIKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYKLCEEWNLAVDRIPDK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNPNQHEVKRLLP 627

BLAST of Carg07943 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.3e-199
Identity = 399/658 (60.64%), Postives = 490/658 (74.47%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG C SR++  E VSRCKAR+RY+K LVKARQ  S SH+LY+RSLR  G++L  FS++ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTAL-----PPPP 120
            +H  LHH+     PSP+P  PPPPP PPPPP+     + TWT+TT S+ L     PPPP
Sbjct: 61  PLH--LHHN----PPSPSP-PPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPPPPPPPPP 120

Query: 121 PPPPSSTWDFWDPFV-PSTSRTEEEWE---------ATTIASEARITVTGAASSAPPPSV 180
           PPPPSSTWDFWDPF+ P  S +EEEWE         AT   S+A +T     ++    SV
Sbjct: 121 PPPPSSTWDFWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTAPTTATPQASSV 180

Query: 181 VSGFSKDASST-----ELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPT-- 240
           VSGFSKD  +T     ELAVVVSRN KDL+EIIKE+DEYFLKAA++GA LS LLE+ T  
Sbjct: 181 VSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLEISTSI 240

Query: 241 --FSSQNKGGQVYSN---RWSLSPSLRVWGSNTKPDALGRLNGQLTATNMGNGYTGNS-- 300
             FS  +K G++YS+     +L+P+   W     P  L          N G    GN   
Sbjct: 241 TDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPSKLSEYR------NAGGVIGGNCIV 300

Query: 301 GSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEK 360
           GSH STV++LYAWEKKLYQEVK AE+I+++HEKKV+ +R+LE++RA+Y+KTEK KK+VEK
Sbjct: 301 GSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEK 360

Query: 361 LESQMMVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLK 420
           LESQ+ V+SQAI++ S EIIKLRE ELYPQL+E VKGLMCMWRSMYE HQVQTHIV+QLK
Sbjct: 361 LESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLK 420

Query: 421 YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISG 480
           YLN +PS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWLRLSLFQ S 
Sbjct: 421 YLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLFQFSK 480

Query: 481 NPLLRTDQHSRIYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKAD 540
           NPL+R+   S+IY  CEEW+LA+DRIPDKVASEGIKSFLT +H IV QQA E++Q+K+ +
Sbjct: 481 NPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKRTE 540

Query: 541 SASKELDKRANELRSLESKYVSHSMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYE 600
           S  K+ +K++  LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ KA +EK K+E
Sbjct: 541 SMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGKAEEEKSKHE 600

Query: 601 SLISVTRSMTIYNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKN--SNQHEVKRLLP 628
             +SVTR+MT+ NLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+   +Q EVKRLLP
Sbjct: 601 KSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQEEVKRLLP 640

BLAST of Carg07943 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.4e-82
Identity = 266/769 (34.59%), Postives = 371/769 (48.24%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG   S++E+ +TV RCK R R+MK+ V +RQ  +++H+ Y+RSLR T AAL +F+    
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  YIHRPLHHHLPP-------------------------EVPSPTP-------RTPPPPPT- 120
            +   + HH  P                          +P PTP         PPPPPT 
Sbjct: 61  SL--AVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQ 120

Query: 121 ---PPPPPIPMSPS------------SDTWTSTTASTALPPP--PPPPPSSTWDFWDPFV 180
              PPPP    +P             SD+  ++ A ++   P    P  SS WD W+ F 
Sbjct: 121 SHQPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTPSSSSAWD-WENFY 180

Query: 181 PST------------------------------------------------SRTEEE--- 240
           P +                                                   EEE   
Sbjct: 181 PPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDDEREEEMHC 240

Query: 241 --WE------ATTIASEARI---------------------TVTGAASSAPPPSVV---- 300
             WE      A+T  SE R                      T     ++AP P  +    
Sbjct: 241 GGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPLPLPLRRRD 300

Query: 301 -SGFSKDASST-----ELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFS 360
               + D+SST     E+ +V+   T  L EI+  ++EYF+KAAEAG G+S LLE     
Sbjct: 301 ERSEAGDSSSTVTAAAEMRMVIRHRT--LAEIVAAIEEYFVKAAEAGNGVSELLEASRAQ 360

Query: 361 SQNKGGQ----VYSNRWSLSPSLRVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCS 420
                 Q    VY +   LS     W S  KP    R      A  M    +    SH S
Sbjct: 361 LDRNFRQLKKTVYHSNSLLSSLSSTWTS--KPPLAVRYKLDTNALEM---ESMEGKSHGS 420

Query: 421 TVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQM 480
           T+E+L AWEKKLYQEVK  E+++IEHEKK+  L+ LE R  D  K +KTK  + KL+S +
Sbjct: 421 TLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQSLI 480

Query: 481 MVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNIL 540
           +V SQA  TTS+ I+++R+ EL PQL+E    L+ MWRSM   H++Q  IV+Q++ L   
Sbjct: 481 IVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGLVDN 540

Query: 541 PSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGN--PL 600
             AE TS++HR +T  LE  V  WH +F  L+K QRDY+++L GWL+L+LFQ+  N    
Sbjct: 541 SMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTLFQVDSNIPQE 600

Query: 601 LRTDQHSR-IYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKADSA 611
             T   SR +   C+EW  A+DR+PD  ASE IKSF+ V+H I  +QA E + +K+ ++ 
Sbjct: 601 AYTSLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIYTKQAEEMKIKKRTETY 660

BLAST of Carg07943 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.5e-70
Identity = 257/797 (32.25%), Postives = 368/797 (46.17%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE- 60
           MG   S+L+  + V RCK R R MK+ V AR   +A+H+ Y RSLR TG+AL  F++ E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------TYIHRP--------LHHHLPPEV-PSPTPRTPPPPPTPP-----PPPIPMS 120
                     ++H P            +PP   PSP P +  PP T P       P  MS
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 PSSDTWTSTTASTALPP------------------------------------------- 180
            SS+          LP                                            
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 ------PPPPPPS---------------------------STWDFWD-------PFVPST 240
                 PP PP S                           S +DF+D        F    
Sbjct: 181 NWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFESMR 240

Query: 241 SRTEE------------EWE-----ATTIASEA-----------RITVTGAASS------ 300
           ++ EE            EWE     +TT +S+A            I+  G  S       
Sbjct: 241 NQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTVR 300

Query: 301 -----------APPPSVVSG-----FSKDASST-------------ELAVVVSRNTKDLV 360
                      +P P V  G     + K   +T             ++ +VV    +DL 
Sbjct: 301 SNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRH--RDLK 360

Query: 361 EIIKELDEYFLKAAEAGAGLSVLLEVPTFS-----SQNKGGQVYSNRWSLSPSLRVWGSN 420
           EII  + E F KAA +G  +S +LE+         SQ K   ++S+   LS     W S 
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSS-LLSNLSSTWTS- 420

Query: 421 TKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKK 480
            KP    +     TA +  N    +S S CST+++L AWEKKLY+E+K  E  +IEHEKK
Sbjct: 421 -KPPLAVKYRIDTTALDQPN----SSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKK 480

Query: 481 VDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYPQLLEF 540
           +  L+  E +  D  K +KTK  + +L+S ++V SQA+ TTST II+LR+ +L PQL+E 
Sbjct: 481 LSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVEL 540

Query: 541 VKGLMCMWRSMYECHQVQTHIVEQLK-YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSF 600
             G M MW+SM++ H+ Q  IVEQ++  +N     E TSE+HRQ+T  LE  V  WH SF
Sbjct: 541 CHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSF 600

Query: 601 CNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHS-RIYELCEEWNLAVDRIPDKVAS 611
            +L+K QRD++ S+  W +L+L  +         +     Y  C+EW LA+DRIPD VAS
Sbjct: 601 SSLIKFQRDFIHSVHAWFKLTLLPVCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTVAS 660

BLAST of Carg07943 vs. ExPASy TrEMBL
Match: A0A6J1EI04 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433579 PE=4 SV=1)

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 624/627 (99.52%), Postives = 625/627 (99.68%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNLVKAQ+DYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK
Sbjct: 421 HGSFCNLVKAQQDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKAD ASKELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADYASKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNPNQHEVKRLLP 627

BLAST of Carg07943 vs. ExPASy TrEMBL
Match: A0A6J1EEF6 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433579 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 624/628 (99.36%), Postives = 625/628 (99.52%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET
Sbjct: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKG-GQVYSNRWSLSPSL 240
           VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKG GQVYSNRWSLSPSL
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGAGQVYSNRWSLSPSL 240

Query: 241 RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300
           RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR
Sbjct: 241 RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300

Query: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360
           IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY
Sbjct: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360

Query: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420
           PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ
Sbjct: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420

Query: 421 WHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD 480
           WHGSFCNLVKAQ+DYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD
Sbjct: 421 WHGSFCNLVKAQQDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD 480

Query: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMREC 540
           KVASEGIKSFLTVIHAIVVQQAAENRQRKKAD ASKELDKRANELRSLESKYVSHSMREC
Sbjct: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADYASKELDKRANELRSLESKYVSHSMREC 540

Query: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600
           SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF
Sbjct: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600

Query: 601 SSVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SSVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SSVCMHAYEAVYNQTKNPNQHEVKRLLP 628

BLAST of Carg07943 vs. ExPASy TrEMBL
Match: A0A6J1KQG2 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497327 PE=4 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 613/627 (97.77%), Postives = 620/627 (98.88%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLE+GETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE+
Sbjct: 1   MGSCYSRLEKGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEES 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           Y+H PLHHHLPPEVPSPTPRTPPPPPT PPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YVHPPLHHHLPPEVPSPTPRTPPPPPTLPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLSPSLR 240
           VVSRNTKDLVEIIKELDEYFLKAAEA AGLSVLLE+PTFSSQNKGGQVYSNRWSLSP LR
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEASAGLSVLLEIPTFSSQNKGGQVYSNRWSLSPKLR 240

Query: 241 VWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300
           VWGSNTKPDA G+LNGQLTATNMG GYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI
Sbjct: 241 VWGSNTKPDAFGKLNGQLTATNMGTGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRI 300

Query: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360
           EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP
Sbjct: 301 EHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYP 360

Query: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420
           QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW
Sbjct: 361 QLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQW 420

Query: 421 HGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPDK 480
           HGSFCNL+KAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIY+LCEEWNLAVDRIPDK
Sbjct: 421 HGSFCNLIKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYKLCEEWNLAVDRIPDK 480

Query: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540
           VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS
Sbjct: 481 VASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECS 540

Query: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600
           ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS
Sbjct: 541 ASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGFS 600

Query: 601 SVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SVCMHAYEAVYNQTKNPNQHEVKRLLP 627

BLAST of Carg07943 vs. ExPASy TrEMBL
Match: A0A6J1KT00 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497327 PE=4 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 613/628 (97.61%), Postives = 620/628 (98.73%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MGSCYSRLE+GETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE+
Sbjct: 1   MGSCYSRLEKGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEES 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120
           Y+H PLHHHLPPEVPSPTPRTPPPPPT PPPPIPMSPSSDTWTSTTASTALPPPPPPPPS
Sbjct: 61  YVHPPLHHHLPPEVPSPTPRTPPPPPTLPPPPIPMSPSSDTWTSTTASTALPPPPPPPPS 120

Query: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180
           STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV
Sbjct: 121 STWDFWDPFVPSTSRTEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASSTELAV 180

Query: 181 VVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKG-GQVYSNRWSLSPSL 240
           VVSRNTKDLVEIIKELDEYFLKAAEA AGLSVLLE+PTFSSQNKG GQVYSNRWSLSP L
Sbjct: 181 VVSRNTKDLVEIIKELDEYFLKAAEASAGLSVLLEIPTFSSQNKGAGQVYSNRWSLSPKL 240

Query: 241 RVWGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300
           RVWGSNTKPDA G+LNGQLTATNMG GYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR
Sbjct: 241 RVWGSNTKPDAFGKLNGQLTATNMGTGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIR 300

Query: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360
           IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY
Sbjct: 301 IEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELY 360

Query: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420
           PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ
Sbjct: 361 PQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLELEVQQ 420

Query: 421 WHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAVDRIPD 480
           WHGSFCNL+KAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIY+LCEEWNLAVDRIPD
Sbjct: 421 WHGSFCNLIKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYKLCEEWNLAVDRIPD 480

Query: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMREC 540
           KVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMREC
Sbjct: 481 KVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMREC 540

Query: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600
           SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF
Sbjct: 541 SASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQAMVGF 600

Query: 601 SSVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           SSVCMHAYEAVYNQTKN NQHEVKRLLP
Sbjct: 601 SSVCMHAYEAVYNQTKNPNQHEVKRLLP 628

BLAST of Carg07943 vs. ExPASy TrEMBL
Match: A0A5A7SHM1 (Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001120 PE=4 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 1.1e-302
Identity = 562/633 (88.78%), Postives = 585/633 (92.42%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG CYSRLER ETVSRCKAR+RYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSN ET
Sbjct: 69  MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 128

Query: 61  YIHRP---LHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTALPPPPPP 120
           YI RP    HHHLPP +PSPTPRTPPP   PPPP IPMSPSSDTWTS TAS ALPPPPPP
Sbjct: 129 YIRRPQPHQHHHLPPVLPSPTPRTPPP---PPPPHIPMSPSSDTWTSITASPALPPPPPP 188

Query: 121 PPSSTWDFWDPFVPSTSR--TEEEWEATTIASEARITVTGAASSAPPPSVVSGFSKDASS 180
           PPSSTWDFWDPFVPSTSR  TEEEWEATTIASEA +TVTGAASSAPPPSVVSGFSKD SS
Sbjct: 189 PPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDTSS 248

Query: 181 TELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSL 240
           TELA+VVSRNTKDLVEIIKELDEYFLKAA+AGA LS+LLEVPTFSSQ KGGQVY+N WSL
Sbjct: 249 TELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGWSL 308

Query: 241 SPSLRVWGSNTKP-DALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKI 300
                 WGSNTKP DA G+LNG++ ATNMGNGY GNSGSHCSTVEKLYAWEKKLYQEVK 
Sbjct: 309 ------WGSNTKPNDAFGKLNGEIIATNMGNGYGGNSGSHCSTVEKLYAWEKKLYQEVKT 368

Query: 301 AEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLR 360
           AEA RIEHEKKV+ LRKLEL+RADY+KTEKTKKEVEKLESQMMVASQAIETTS EIIKLR
Sbjct: 369 AEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIKLR 428

Query: 361 EIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTLQLE 420
           EIELYPQLLE VKGLMCMWRSMYE HQVQTHIVEQLKYLNI+PSAEPTSEIHRQSTLQLE
Sbjct: 429 EIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQLE 488

Query: 421 LEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWNLAV 480
           LEVQQWH SFCNLVKAQRDYVQSLTGWLRL LFQISGNPLLRT Q SRIY LCEEWNLAV
Sbjct: 489 LEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNLAV 548

Query: 481 DRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANELRSLESKYVSH 540
           DRIPDKVASEGIKSFLTV+HAIVVQQA E RQ+KKADSASKELDKRA ELRSLESKYVSH
Sbjct: 549 DRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYVSH 608

Query: 541 SMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQMSFPHVFQ 600
           SMRECSASTRGRDPVQEK+NKV+NLRTKA+DEKQK+E+L+SVTRSMTI NLQMSFPHVFQ
Sbjct: 609 SMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHVFQ 668

Query: 601 AMVGFSSVCMHAYEAVYNQTKNSNQHEVKRLLP 628
           AMVGFSSVCMHAYEAVYNQTKN +QHEVKRLLP
Sbjct: 669 AMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 692

BLAST of Carg07943 vs. TAIR 10
Match: AT3G51290.2 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 689.1 bits (1777), Expect = 3.3e-198
Identity = 392/656 (59.76%), Postives = 486/656 (74.09%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG C SR++  E VSRCKAR+RY+K LVKARQ  S SH+LY+RSLR  G++L  FS++ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTAL-----PPPP 120
            +H  LHH+     PSP+P  PPPPP PPPPP+     + TWT+TT S+ L     PPPP
Sbjct: 61  PLH--LHHN----PPSPSP-PPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPPPPPPPPP 120

Query: 121 PPPPSSTWDFWDPFV-PSTSRTEEEWE---------ATTIASEARITVTGAASSAPPPSV 180
           PPPPSSTWDFWDPF+ P  S +EEEWE         AT   S+A +T     ++    SV
Sbjct: 121 PPPPSSTWDFWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTAPTTATPQASSV 180

Query: 181 VSGFSKDASST-----ELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPT-- 240
           VSGFSKD  +T     ELAVVVSRN KDL+EIIKE+DEYFLKAA++GA LS LLE+ T  
Sbjct: 181 VSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLEISTSI 240

Query: 241 --FSSQNKGGQVYSN---RWSLSPSLRVWGSNTKPDALGRLNGQLTATNMGNGYTGNS-- 300
             FS  +K G++YS+     +L+P+   W     P  L          N G    GN   
Sbjct: 241 TDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPSKLSEYR------NAGGVIGGNCIV 300

Query: 301 GSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEK 360
           GSH STV++LYAWEKKLYQEVK AE+I+++HEKKV+ +R+LE++RA+Y+KTEK KK+VEK
Sbjct: 301 GSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEK 360

Query: 361 LESQMMVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLK 420
           LESQ+ V+SQAI++ S EIIKLRE ELYPQL+E VKGLMCMWRSMYE HQVQTHIV+QLK
Sbjct: 361 LESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLK 420

Query: 421 YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISG 480
           YLN +PS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWLRLSLFQ S 
Sbjct: 421 YLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLFQFSK 480

Query: 481 NPLLRTDQHSRIYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKAD 540
           NPL+R+   S+IY  CEEW+LA+DRIPDKVASEGIKSFLT +H IV QQA E++Q+K+ +
Sbjct: 481 NPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKRTE 540

Query: 541 SASKELDKRANELRSLESKYVSHSMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYE 600
           S  K+ +K++  LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ KA +EK K+E
Sbjct: 541 SMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGKAEEEKSKHE 600

Query: 601 SLISVTRSMTIYNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKNSNQHEVKRLLP 628
             +SVTR+MT+ NLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+  + + +  +P
Sbjct: 601 KSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQEEDYVP 638

BLAST of Carg07943 vs. TAIR 10
Match: AT3G51290.1 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 676.8 bits (1745), Expect = 1.7e-194
Identity = 393/658 (59.73%), Postives = 484/658 (73.56%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG C SR++  E VSRCKAR+RY+K LVKARQ  S SH+LY+RSLR  G++L  FS++ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIHRPLHHHLPPEVPSPTPRTPPPPPTPPPPPIPMSPSSDTWTSTTASTAL-----PPPP 120
            +H  LHH+     PSP+P  PPPPP PPPPP+     + TWT+TT S+ L     PPPP
Sbjct: 61  PLH--LHHN----PPSPSP-PPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPPPPPPPPP 120

Query: 121 PPPPSSTWDFWDPFV-PSTSRTEEEWE---------ATTIASEARITVTGAASSAPPPSV 180
           PPPPSSTWDFWDPF+ P  S +EEEWE         AT   S+A +T     ++    SV
Sbjct: 121 PPPPSSTWDFWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTAPTTATPQASSV 180

Query: 181 VSGFSKDASST-----ELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPT-- 240
           VSGFSKD  +T     ELAVVVSRN KDL+EIIKE+DEYFLKAA++GA LS LLE+ T  
Sbjct: 181 VSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLEISTSI 240

Query: 241 --FSSQNKGGQVYSN---RWSLSPSLRVWGSNTKPDALGRLNGQLTATNMGNGYTGNS-- 300
             FS  +K G++YS+     +L+P+   W     P  L          N G    GN   
Sbjct: 241 TDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPSKLSEYR------NAGGVIGGNCIV 300

Query: 301 GSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEK 360
           GSH STV++LYAWEKKLYQEVK AE+I+++HEKKV+ +R+LE++RA+Y+KTEK KK+VEK
Sbjct: 301 GSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEK 360

Query: 361 LESQMMVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLK 420
           LESQ+ V+SQAI++ S EIIKLRE ELYPQL+E VKG      SMYE HQVQTHIV+QLK
Sbjct: 361 LESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKG------SMYESHQVQTHIVQQLK 420

Query: 421 YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISG 480
           YLN +PS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWLRLSLFQ S 
Sbjct: 421 YLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLFQFSK 480

Query: 481 NPLLRTDQHSRIYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKAD 540
           NPL+R+   S+IY  CEEW+LA+DRIPDKVASEGIKSFLT +H IV QQA E++Q+K+ +
Sbjct: 481 NPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKRTE 540

Query: 541 SASKELDKRANELRSLESKYVSHSMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYE 600
           S  K+ +K++  LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ KA +EK K+E
Sbjct: 541 SMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGKAEEEKSKHE 600

Query: 601 SLISVTRSMTIYNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKN--SNQHEVKRLLP 628
             +SVTR+MT+ NLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+   +Q EVKRLLP
Sbjct: 601 KSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQEEVKRLLP 634

BLAST of Carg07943 vs. TAIR 10
Match: AT1G02110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 295.8 bits (756), Expect = 8.1e-80
Identity = 251/731 (34.34%), Postives = 363/731 (49.66%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFS---- 60
           MG   S+L+  + V RCK R R MK  V AR   +A+HS Y RSLR TG+AL  F+    
Sbjct: 1   MGCTASKLDSEDAVRRCKERRRLMKDAVYARHHLAAAHSDYCRSLRLTGSALSSFAAGEP 60

Query: 61  -----NEETYIHRPLHHHLPPEVPSPTPRTPPPPP--TPPPPPIP---------MSPSSD 120
                N      RP      P VPS     PPPPP  + P P  P          SPSS 
Sbjct: 61  LSVSENTPAVFLRPSSSQDAPRVPSSHSPEPPPPPIRSKPKPTRPRRLPHILSDSSPSSS 120

Query: 121 TWTS---TTASTALPPPPPPPPSSTWDFWDPFVPST----------------SRTEEEWE 180
             TS   T    +     P   SS W+ W+ F P +                 R   +++
Sbjct: 121 PATSFYPTAHQNSTYSRSPSQASSVWN-WENFYPPSPPDSEYFERKARQNHKHRPPSDYD 180

Query: 181 ATTIASE-----------------------ARITVTGAASS------------------A 240
           A T  S+                        R T T ++                     
Sbjct: 181 AETERSDHDYCHSRRDAAEEVHCSEWGDDHDRFTATSSSDGDGEVETHVSRSGIEEEPVK 240

Query: 241 PPPSVVSG--------FSKDASSTELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSV 300
            P    +G         S D   T+L V      K+L EI+  + +YF KAA AG  +S 
Sbjct: 241 QPHQDPNGKEHSDHVTTSSDCYKTKLVV----RHKNLKEILDAVQDYFDKAASAGDQVSA 300

Query: 301 LLEV------PTFSSQNK----GGQVYSN---RWSLSPSLRVWGSNTKPDALGRLNGQLT 360
           +LE+       +FS   K       V+SN    W+  P L V               +L 
Sbjct: 301 MLEIGRAELDRSFSKLRKTVYHSSSVFSNLSASWTSKPPLAV-------------KYKLD 360

Query: 361 ATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKKVDLLRKLELRRADY 420
           A+ + N   G   S CST+++L AWEKKLY++VK  E ++IEHEKK+  L+  E +  D 
Sbjct: 361 ASTL-NDEQGGLKSLCSTLDRLLAWEKKLYEDVKAREGVKIEHEKKLSALQSQEYKGGDE 420

Query: 421 LKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYPQLLEFVKGLMCMWRSMYEC 480
            K +KTK  + +L+S ++V+S+A+ TTS  I++LR+ +L PQL+E   GLM MW+SM+E 
Sbjct: 421 SKLDKTKTSITRLQSLIIVSSEAVLTTSNAILRLRDTDLVPQLVELCHGLMYMWKSMHEY 480

Query: 481 HQVQTHIVEQLK-YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSFCNLVKAQRDYVQSL 540
           H++Q +IV+Q++  +N     E TSE+HRQ T  LE  V  WH SFC ++K QR+++ SL
Sbjct: 481 HEIQNNIVQQVRGLINQTERGESTSEVHRQVTRDLESAVSLWHSSFCRIIKFQREFICSL 540

Query: 541 TGWLRLSLFQIS-GNPLLRTDQHSRIYELCEEWNLAVDRIPDKVASEGIKSFLTVIHAIV 600
             W +LSL  +S G+P     Q    + LCEEW  +++R+PD VASE IKSF+ V+H I 
Sbjct: 541 HAWFKLSLVPLSNGDP---KKQRPDSFALCEEWKQSLERVPDTVASEAIKSFVNVVHVIS 600

Query: 601 VQQAAENRQRKKADSASKELDKRANELRSLESKYVSHSMRECSASTRG----------RD 619
           ++QA E + +K+ +SA KEL+K+A+ LRS+E KY        + ST G          RD
Sbjct: 601 IKQAEEVKMKKRTESAGKELEKKASSLRSIERKYYQ------AYSTVGIGPGPEVLDSRD 660

BLAST of Carg07943 vs. TAIR 10
Match: AT3G60320.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 268.9 bits (686), Expect = 1.1e-71
Identity = 257/797 (32.25%), Postives = 368/797 (46.17%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEE- 60
           MG   S+L+  + V RCK R R MK+ V AR   +A+H+ Y RSLR TG+AL  F++ E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------TYIHRP--------LHHHLPPEV-PSPTPRTPPPPPTPP-----PPPIPMS 120
                     ++H P            +PP   PSP P +  PP T P       P  MS
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 PSSDTWTSTTASTALPP------------------------------------------- 180
            SS+          LP                                            
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 ------PPPPPPS---------------------------STWDFWD-------PFVPST 240
                 PP PP S                           S +DF+D        F    
Sbjct: 181 NWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFESMR 240

Query: 241 SRTEE------------EWE-----ATTIASEA-----------RITVTGAASS------ 300
           ++ EE            EWE     +TT +S+A            I+  G  S       
Sbjct: 241 NQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTVR 300

Query: 301 -----------APPPSVVSG-----FSKDASST-------------ELAVVVSRNTKDLV 360
                      +P P V  G     + K   +T             ++ +VV    +DL 
Sbjct: 301 SNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRH--RDLK 360

Query: 361 EIIKELDEYFLKAAEAGAGLSVLLEVPTFS-----SQNKGGQVYSNRWSLSPSLRVWGSN 420
           EII  + E F KAA +G  +S +LE+         SQ K   ++S+   LS     W S 
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSS-LLSNLSSTWTS- 420

Query: 421 TKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQEVKIAEAIRIEHEKK 480
            KP    +     TA +  N    +S S CST+++L AWEKKLY+E+K  E  +IEHEKK
Sbjct: 421 -KPPLAVKYRIDTTALDQPN----SSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKK 480

Query: 481 VDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEIIKLREIELYPQLLEF 540
           +  L+  E +  D  K +KTK  + +L+S ++V SQA+ TTST II+LR+ +L PQL+E 
Sbjct: 481 LSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVEL 540

Query: 541 VKGLMCMWRSMYECHQVQTHIVEQLK-YLNILPSAEPTSEIHRQSTLQLELEVQQWHGSF 600
             G M MW+SM++ H+ Q  IVEQ++  +N     E TSE+HRQ+T  LE  V  WH SF
Sbjct: 541 CHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSF 600

Query: 601 CNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHS-RIYELCEEWNLAVDRIPDKVAS 611
            +L+K QRD++ S+  W +L+L  +         +     Y  C+EW LA+DRIPD VAS
Sbjct: 601 SSLIKFQRDFIHSVHAWFKLTLLPVCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTVAS 660

BLAST of Carg07943 vs. TAIR 10
Match: AT2G34670.2 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 267.3 bits (682), Expect = 3.1e-71
Identity = 227/697 (32.57%), Postives = 341/697 (48.92%), Query Frame = 0

Query: 1   MGSCYSRLERGETVSRCKARERYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNEET 60
           MG   SR++  E V  C+ R+R MK+L+  R  F+ +   Y+R+LR TG  LRQF+  ET
Sbjct: 1   MGCAASRIDNEEKVLVCRQRKRLMKKLLGFRGEFADAQLAYLRALRNTGVTLRQFTESET 60

Query: 61  YIHRPLHHHLP-PEVPSPTPRTPPPPPTPPP--PPI-------PMSPSSDTWTSTTASTA 120
                  + L  P  PSP P  PP PP PPP  P +        ++   +   +   +  
Sbjct: 61  LELENTSYGLSLPLPPSPPPTLPPSPPPPPPFSPDLRNPETSHDLADEEEEGENDGGNDG 120

Query: 121 LPPPPPPPPSSTWDFWDPFV-------PSTSRT----------------EEEWEATTIAS 180
               PPPP  ++W+ W+PF        P+                    EE+W  T    
Sbjct: 121 SGAAPPPPLPNSWNIWNPFESLELHSHPNGDNVVTQVELKKKQQIQQAEEEDWAETKSQF 180

Query: 181 EAR------------------ITVTGAASSAP-------------PPSVVSGFSKDASST 240
           E                      V+G     P               S+ S   KD   T
Sbjct: 181 EEEDEQQEAGGTCLDLSVHQIEAVSGCNMKKPRRLKFKLGEVMDGNSSMTSCSGKDLEKT 240

Query: 241 ELAVVVSRNTKDLVEIIKELDEYFLKAAEAGAGLSVLLEVPTFSSQNKGGQVYSNRWSLS 300
              V   R  + L  II+ELD+YFLKA+     ++V++++ +  + +   +    R   S
Sbjct: 241 H--VTDCRIRRTLEGIIRELDDYFLKASGCEKEIAVIVDINSRDTVDP-FRYQETRRKRS 300

Query: 301 PSLRV-----WGSNTKPDALGRLNGQLTATNMGNGYTGNSGSHCSTVEKLYAWEKKLYQE 360
            S +V     W  ++K   LG+      AT  G       G+HCST+EKLY  EKKLYQ 
Sbjct: 301 SSAKVFSALSWSWSSKSLQLGK-----DATTSGTVEPCRPGAHCSTLEKLYTAEKKLYQL 360

Query: 361 VKIAEAIRIEHEKKVDLLRKLELRRADYLKTEKTKKEVEKLESQMMVASQAIETTSTEII 420
           V+  E  ++EHE+K  LL+K +    D  K EK +  +E LE+++     +I TT + ++
Sbjct: 361 VRNKEIAKVEHERKSALLQKQDGETYDLSKMEKARLSLESLETEIQRLEDSITTTRSCLL 420

Query: 421 KLREIELYPQLLEFVKGLMCMWRSMYECHQVQTHIVEQLKYLNILPSAEPTSEIHRQSTL 480
            L   ELYPQL+    GL  MW++M +CHQVQ HI +QL +L   PS + +SE  RQ+  
Sbjct: 421 NLINDELYPQLVALTSGLAQMWKTMLKCHQVQIHISQQLNHLPDYPSIDLSSEYKRQAVN 480

Query: 481 QLELEVQQWHGSFCNLVKAQRDYVQSLTGWLRLSLFQISGNPLLRTDQHSRIYELCEEWN 540
           +LE EV  W+ SFC LV +QR+YV++L  W++L+  ++S     R+       +LC+EW 
Sbjct: 481 ELETEVTCWYNSFCKLVNSQREYVKTLCTWIQLT-DRLSNEDNQRSSLPVAARKLCKEWQ 540

Query: 541 LAVDRIPDKVASEGIKSFLTVIHAIVVQQAAENRQRKKADSASKELDKRANEL----RSL 600
           L  +++PDKV SE IKSFL  I +I+ QQA E   R+K +   + L+K    L    R L
Sbjct: 541 LVFEKLPDKVTSEAIKSFLMSIKSIIHQQAEEYNLRRKCNKLERRLEKELISLAEIERRL 600

Query: 601 ESKYVSHSMRECSASTRGRDPVQEKKNKVENLRTKARDEKQKYESLISVTRSMTIYNLQM 621
           E           S S   + P+  K+ K+E LR +   EK KY + + V++ MT+ NL+ 
Sbjct: 601 EGILAMEEEEVSSTSLGSKHPLSIKQAKIEALRKRVDIEKTKYLNSVEVSKRMTLDNLKS 660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6594763.10.0e+00100.00Protein ALTERED PHOSPHATE STARVATION RESPONSE 1, partial [Cucurbita argyrosperma... [more]
XP_022926423.10.0e+0099.52nitrate regulatory gene2 protein-like isoform X2 [Cucurbita moschata][more]
XP_022926422.10.0e+0099.36nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata][more]
XP_023518366.10.0e+0098.25nitrate regulatory gene2 protein-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023003866.10.0e+0097.77nitrate regulatory gene2 protein-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A178VBJ01.3e-19960.64Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9AQW16.4e-8234.59Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
Q93YU81.5e-7032.25Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1EI040.0e+0099.52nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1EEF60.0e+0099.36nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1KQG20.0e+0097.77nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1KT000.0e+0097.61nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A5A7SHM11.1e-30288.78Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-con... [more]
Match NameE-valueIdentityDescription
AT3G51290.23.3e-19859.76Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) [more]
AT3G51290.11.7e-19459.73Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) [more]
AT1G02110.18.1e-8034.34Protein of unknown function (DUF630 and DUF632) [more]
AT3G60320.11.1e-7132.25Protein of unknown function (DUF630 and DUF632) [more]
AT2G34670.23.1e-7132.57Protein of unknown function (DUF630 and DUF632) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 322..349
NoneNo IPR availableCOILSCoilCoilcoord: 510..530
NoneNo IPR availableCOILSCoilCoilcoord: 549..576
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..97
NoneNo IPR availablePANTHERPTHR21450:SF23SERINE HYDROXYMETHYLTRANSFERASE, PUTATIVE (DUF632)-RELATEDcoord: 1..615
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..615
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..59
e-value: 3.5E-20
score: 71.9
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 189..494
e-value: 8.8E-102
score: 340.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07943-RACarg07943-RAmRNA