Carg07613 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07613
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAcyl-[acyl-carrier-protein] hydrolase
LocationCarg_Chr13: 8130290 .. 8132812 (-)
RNA-Seq ExpressionCarg07613
SyntenyCarg07613
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCATTGCAGCATCATATTTGATTCCGAGAACCCGCCGTGATTTGACGTGGGGACGTAACTCCTCCGTCGTGATCGCCACCGCCGGTGGCAGCCGCAGCGCCCTACGTAGCGTGGACTTGGTAAATGGAAAGAAGGTAAATGGGGTCCACATCCCCGGCGAAAAAAAGGGATCTTTATTCATGGAAGAACCACACGTTGAAGGCAATAAGCCTCTCCATGATTGTCTTCTAGGAAGGTTCGTGGAAGAAAACTTCGTTTATGGCCAAACCTTCATCATTAGGTCCTACGAAATAGGACCCGATAAAACCGCCACTATGGAAACCCTCATGAACCTTCTTCAGGTATGATATGATATGATACTGTCCACTTTCAGTGCAGTACTAAATTGATTGTATTCCTGACAGGAAACAGCCCTGAATCATGTAACGTGTTCAGGGCTGGCTGGTAATGACTTTGGGGCCACACGGGAGATGAGCCTCCGGAAATTGATTTGGGTTGTGACACGGGTTCATGTCCAAGTTCAAAGATATAGCTGCTGGTTGGTTTGTTTTGTTTAGACAAAACCCTTTTGAATTTTGTGAATCAAAAATTGAAACTTTGTGATGATGCAGGGGAGATGTGGTTGAGATAGACACATGGGTTGATGCAGCCGGCAAAAATGGGATGAGGCGTGATTGGATCATAAGAGATTATCATACTCGAGAGATTATTACAAGAGCCACCAGGTTTGTAGGTTTCGCGCCATTAATATTCATGCAAATATGTGCCCTAATGAATGGCCTAATATGGGACATTTTTTGGGATTTTCAGTACATGGGTTATAATGAATAGAGAAACGAGAAGGTTGTCGAAAATACCCGACCAAGTACGAGAAGAGTTGACACCGTTTTACCTTAATAGAATTGCCATCCCTACTGACAAAGACGACAGTGACAAGATTGACAAGCTTACTGATGAAACTGCCGACAGAATCCGGTCAGGCTTAGCTGTAAGCATCTTATTTCTTGACCTATTGATTTGATATGATGTTTAGTGTAGGAATGCAGTAGAAAATGATTTTTTTTTCACCTATTACTTCAAACCCATTAGGGGATTGTTAATTTGAATTTGAGGTTTGATGATGGTATCCATGTTCTAGCCACGTTACTTGCTTTTCGTGTATTAATACGGATCGTTTCATGTGGGATTTCATGGTGGTTAGAGAGGTTAACAAAATATTATTTATATGGGTGTGTAAATCTATATGTGTATACGCGTTTTGAAATTTGGAGGGAATACCCAGAAGAGAAAGTCTAAAAAGGACAATATTTGTAATAGTTTAAGCTTACCACTAGCCGATATTGTCATTTCTGAGCTTTCTCTTATGGGGTTCCTCTCAAGGTTTTAAAACGTCACTTCTAGGGAGAGGTTTCCACACTGTTACAAATAATGTTTTGTTCCCCTCTCCATGTGGGATCTCACAATATCTAGCAGTGAGCTCGGATTGTTACAAATGGTATTTGAGCAAGCATTGGGCCGATGCTAACCCTCAAGGGGTAGATTGTGAGATCCCACCTCGGTTGGAGAGAAAGCCCAAAAGGGAAAGCCTAGAAAGGAAAGCACAAAGAGAATATCGATAATAGCCCAAACCCACCCTAGTAGATATTGCCCTCCTTGAGTTTTCTCTTCTAGGCTTCCTCTCAAGGTTTTAAAATGCGTTTTCTAGGGGGAGATTTCTACATTCTTATAAGTAATGTTTCGTTTTTCTCTCCAACCAATGTGGGATTTCACGATATCTAATAGCAGTGAGCTTGAGTTGTTACACATGGTATCAGAGCCAGATACCGCGCGGTGTGCCAGCGAGAATGCTAGTCCTCGTGGAGGTGGATAGTGAGATTCCACCTCGGTTGGAGAGGGGAACCAAACGTCTTTATATGGGTGTGAAAACCTCTCCTCTCTCCCTCATAGACTTGTTTTAAAATCTTCAAATAAAACTCAAAAGGAGAAACATGCTAAAAAAAACATGGGAATAGTTTTCCGTTTCAAAAGCAAGAAGTGAGTAACATCACTTAGTGAGTCGATGCAAGTGAATATCGAAAACATCAAAGTTTAGAATTGAGATTGATATCTTACGTGCAGCCTAGATGGAACGACATGGATGCGAACCAGCACGTGAACAACGTTAAATACATAGGGTGGATTCTGGAGGTAACAAACCAATCCCCATTAGTTTGGACAGAAATTGAATATGTTGTTAATAACAAAACTATTGGTTGCAGAGTGTACCAATAAACGTGCTGGAAGATTATGATTTAACGAGCATGACATTGGAGTATCGAAGAGAGTGCAGGCAGTCCAATCTGCTGGAGTCCTTAACAAACACCACCACCGCCGCCGCCGCCGCCGCCGCCAGTGCTGCAATAGAAGAGCCTCCCAAGAACAACCTCCAATACACCCACCTCCTTCGCATGCAACCCAACAAGGCTGAAATTGTAAGAGCTAGAACTCAGTGGAACTCCAAGCCCAAACGTTACTAA

mRNA sequence

ATGGCCTCCATTGCAGCATCATATTTGATTCCGAGAACCCGCCGTGATTTGACGTGGGGACGTAACTCCTCCGTCGTGATCGCCACCGCCGGTGGCAGCCGCAGCGCCCTACGTAGCGTGGACTTGGTAAATGGAAAGAAGGTAAATGGGGTCCACATCCCCGGCGAAAAAAAGGGATCTTTATTCATGGAAGAACCACACGTTGAAGGCAATAAGCCTCTCCATGATTGTCTTCTAGGAAGGTTCGTGGAAGAAAACTTCGTTTATGGCCAAACCTTCATCATTAGGTCCTACGAAATAGGACCCGATAAAACCGCCACTATGGAAACCCTCATGAACCTTCTTCAGGAAACAGCCCTGAATCATGTAACGTGTTCAGGGCTGGCTGGTAATGACTTTGGGGCCACACGGGAGATGAGCCTCCGGAAATTGATTTGGGTTGTGACACGGGTTCATGTCCAAGTTCAAAGATATAGCTGCTGGGGAGATGTGGTTGAGATAGACACATGGGTTGATGCAGCCGGCAAAAATGGGATGAGGCGTGATTGGATCATAAGAGATTATCATACTCGAGAGATTATTACAAGAGCCACCAGTACATGGGTTATAATGAATAGAGAAACGAGAAGGTTGTCGAAAATACCCGACCAAGTACGAGAAGAGTTGACACCGTTTTACCTTAATAGAATTGCCATCCCTACTGACAAAGACGACAGTGACAAGATTGACAAGCTTACTGATGAAACTGCCGACAGAATCCGGTCAGGCTTAGCTCCTAGATGGAACGACATGGATGCGAACCAGCACGTGAACAACGTTAAATACATAGGGTGGATTCTGGAGAGTGTACCAATAAACGTGCTGGAAGATTATGATTTAACGAGCATGACATTGGAGTATCGAAGAGAGTGCAGGCAGTCCAATCTGCTGGAGTCCTTAACAAACACCACCACCGCCGCCGCCGCCGCCGCCGCCAGTGCTGCAATAGAAGAGCCTCCCAAGAACAACCTCCAATACACCCACCTCCTTCGCATGCAACCCAACAAGGCTGAAATTGTAAGAGCTAGAACTCAGTGGAACTCCAAGCCCAAACGTTACTAA

Coding sequence (CDS)

ATGGCCTCCATTGCAGCATCATATTTGATTCCGAGAACCCGCCGTGATTTGACGTGGGGACGTAACTCCTCCGTCGTGATCGCCACCGCCGGTGGCAGCCGCAGCGCCCTACGTAGCGTGGACTTGGTAAATGGAAAGAAGGTAAATGGGGTCCACATCCCCGGCGAAAAAAAGGGATCTTTATTCATGGAAGAACCACACGTTGAAGGCAATAAGCCTCTCCATGATTGTCTTCTAGGAAGGTTCGTGGAAGAAAACTTCGTTTATGGCCAAACCTTCATCATTAGGTCCTACGAAATAGGACCCGATAAAACCGCCACTATGGAAACCCTCATGAACCTTCTTCAGGAAACAGCCCTGAATCATGTAACGTGTTCAGGGCTGGCTGGTAATGACTTTGGGGCCACACGGGAGATGAGCCTCCGGAAATTGATTTGGGTTGTGACACGGGTTCATGTCCAAGTTCAAAGATATAGCTGCTGGGGAGATGTGGTTGAGATAGACACATGGGTTGATGCAGCCGGCAAAAATGGGATGAGGCGTGATTGGATCATAAGAGATTATCATACTCGAGAGATTATTACAAGAGCCACCAGTACATGGGTTATAATGAATAGAGAAACGAGAAGGTTGTCGAAAATACCCGACCAAGTACGAGAAGAGTTGACACCGTTTTACCTTAATAGAATTGCCATCCCTACTGACAAAGACGACAGTGACAAGATTGACAAGCTTACTGATGAAACTGCCGACAGAATCCGGTCAGGCTTAGCTCCTAGATGGAACGACATGGATGCGAACCAGCACGTGAACAACGTTAAATACATAGGGTGGATTCTGGAGAGTGTACCAATAAACGTGCTGGAAGATTATGATTTAACGAGCATGACATTGGAGTATCGAAGAGAGTGCAGGCAGTCCAATCTGCTGGAGTCCTTAACAAACACCACCACCGCCGCCGCCGCCGCCGCCGCCAGTGCTGCAATAGAAGAGCCTCCCAAGAACAACCTCCAATACACCCACCTCCTTCGCATGCAACCCAACAAGGCTGAAATTGTAAGAGCTAGAACTCAGTGGAACTCCAAGCCCAAACGTTACTAA

Protein sequence

MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGSLFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKPKRY
Homology
BLAST of Carg07613 vs. NCBI nr
Match: KAG6584162.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019753.1 Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 739.2 bits (1907), Expect = 1.7e-209
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 366

BLAST of Carg07613 vs. NCBI nr
Match: XP_023520196.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 732.3 bits (1889), Expect = 2.1e-207
Identity = 362/366 (98.91%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRR LTWGRNSSVVIATAGGSRS+LRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRGLTWGRNSSVVIATAGGSRSSLRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEEPHVEGNKPLHDCLLGRFVE+NFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEEPHVEGNKPLHDCLLGRFVEQNFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDK+DSD
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKNDSD 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 366

BLAST of Carg07613 vs. NCBI nr
Match: XP_022923931.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 729.9 bits (1883), Expect = 1.1e-206
Identity = 363/366 (99.18%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLTNTTT   AAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTNTTT---AAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 363

BLAST of Carg07613 vs. NCBI nr
Match: XP_023001057.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 724.2 bits (1868), Expect = 5.8e-205
Identity = 358/366 (97.81%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRR LTWGRNSSVVIA+AGGSRS LRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRGLTWGRNSSVVIASAGGSRSGLRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEE HVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEETHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSG+AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGIAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDK+DS+
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKNDSE 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLT+TTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTSTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 366

BLAST of Carg07613 vs. NCBI nr
Match: XP_038895494.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 594.7 bits (1532), Expect = 5.3e-166
Identity = 300/358 (83.80%), Postives = 320/358 (89.39%), Query Frame = 0

Query: 15  RDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHI-------PGEKKGSLFMEEPH 74
           RDL    NS +VIA+  G R++ RSV LVNGKKVNGVH+        GEK+G L +EE  
Sbjct: 47  RDLNRRCNSCIVIAS--GRRTSPRSVGLVNGKKVNGVHVIGEAPLFSGEKRGPLLVEESG 106

Query: 75  VEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSG 134
           V+G KPLHDCLLGRFVE+ FVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSG
Sbjct: 107 VDGEKPLHDCLLGRFVEDKFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSG 166

Query: 135 LAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRD 194
           LAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRD
Sbjct: 167 LAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRD 226

Query: 195 YHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTD 254
           YHTREIITRATSTWVIMN+ETRRLSKIPDQVREELTPFYLNRIAIP+D++DS+KIDKLTD
Sbjct: 227 YHTREIITRATSTWVIMNKETRRLSKIPDQVREELTPFYLNRIAIPSDENDSEKIDKLTD 286

Query: 255 ETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQS 314
           ETA+RIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLED+DLTSMTLEYRRECRQS
Sbjct: 287 ETAERIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDFDLTSMTLEYRRECRQS 346

Query: 315 NLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKPKR 366
           NL+ESLT TT   AA        E  +NNLQYTHLLRMQP+KAEIVRARTQWNSKPK+
Sbjct: 347 NLVESLTTTTATTAA--------EDRRNNLQYTHLLRMQPDKAEIVRARTQWNSKPKQ 394

BLAST of Carg07613 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 8.7e-87
Identity = 158/284 (55.63%), Postives = 212/284 (74.65%), Query Frame = 0

Query: 79  LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATRE 138
           +GR V++  V+ Q F IRSYEIG D++A++ET+MN LQETALNHV  +GL G+ FG+T E
Sbjct: 129 IGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVKTAGLLGDGFGSTPE 188

Query: 139 MSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRAT 198
           M  + LIWVVTR+ V V +Y  WGDVVE+DTWV  +GKNGMRRDW++RD +T E +TRA+
Sbjct: 189 MFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWLVRDCNTGETLTRAS 248

Query: 199 STWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLA 258
           S WV+MN+ TRRLSKIP++VR E+ P+++N  + P   +DS K+ K+ D+TAD +RSGL 
Sbjct: 249 SVWVMMNKLTRRLSKIPEEVRGEIEPYFVN--SDPVLAEDSRKLTKIDDKTADYVRSGLT 308

Query: 259 PRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTT 318
           PRW+D+D NQHVNNVKYIGWILES P+ ++E   L SMTLEYRREC + ++L+SLT  T 
Sbjct: 309 PRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTG 368

Query: 319 AAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
                 A+A        +++  HLLR+Q + AE+VR RT+W+SK
Sbjct: 369 CDIGNLATA-------GDVECQHLLRLQ-DGAEVVRGRTEWSSK 402

BLAST of Carg07613 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 5.3e-84
Identity = 153/284 (53.87%), Postives = 204/284 (71.83%), Query Frame = 0

Query: 79  LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATRE 138
           +G+ V++  V+ Q F IRSYEIG D+TA++ETLMN LQETA+NH   +GL G  FGAT E
Sbjct: 124 IGKIVQDGLVFSQNFSIRSYEIGADQTASIETLMNHLQETAINHCRSAGLLGEGFGATPE 183

Query: 139 MSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRAT 198
           M  + LIWVVTR+ V V RY  WGDVV++DTWV A+GKNGMRRDW++ +  T EI+TRAT
Sbjct: 184 MCKKNLIWVVTRMQVVVDRYPTWGDVVQVDTWVSASGKNGMRRDWLVSNSETGEILTRAT 243

Query: 199 STWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLA 258
           S WV+MN+ TRRLSKIP++VR E+ PF++N  + P   +DS K+ KL D TA+ +  GL 
Sbjct: 244 SVWVMMNKLTRRLSKIPEEVRGEIEPFFMN--SDPVLAEDSQKLVKLDDSTAEHVCKGLT 303

Query: 259 PRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTT 318
           P+W+D+D NQHVNNVKYIGWILES P+ +LE ++L+++TLEYRREC + ++L+SLT  + 
Sbjct: 304 PKWSDLDVNQHVNNVKYIGWILESAPLPILESHELSALTLEYRRECGRDSVLQSLTTVSD 363

Query: 319 AAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
           +    A +              HLLR+  + AEIVR RT+W  K
Sbjct: 364 SNTENAVNV-------GEFNCQHLLRLD-DGAEIVRGRTRWRPK 397

BLAST of Carg07613 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.9e-83
Identity = 155/284 (54.58%), Postives = 201/284 (70.77%), Query Frame = 0

Query: 79  LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATRE 138
           LG  V++  V+ Q F IRSYEIG D+TA++ET+MN LQETALNHV  +GL+ + FG T E
Sbjct: 128 LGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPE 187

Query: 139 MSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRAT 198
           M  R LIWVV ++ V V RY  WGD VE++TWV  +GKNGMRRDW+I D +T EI+TRA+
Sbjct: 188 MYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRAS 247

Query: 199 STWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLA 258
           S WV+MN++TRRLSKIPD+VR E+ P +++  + P  +DD  K+ KL ++TAD IR GL 
Sbjct: 248 SVWVMMNQKTRRLSKIPDEVRNEIEPHFVD--SPPVIEDDDRKLPKLDEKTADSIRKGLT 307

Query: 259 PRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTT 318
           PRWND+D NQHVNNVKYIGWILES P  VLE  +L S+TLEYRREC + ++LESLT    
Sbjct: 308 PRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDP 367

Query: 319 AAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
           +     +            Q+ HLLR++ +  EIV+ RT+W  K
Sbjct: 368 SGGGYGS------------QFQHLLRLE-DGGEIVKGRTEWRPK 396

BLAST of Carg07613 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.9e-82
Identity = 153/284 (53.87%), Postives = 201/284 (70.77%), Query Frame = 0

Query: 79  LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATRE 138
           LGR V++  V+ Q F IRSYEIG D+TA++ET+MN LQETALNHV  +GL+ + FG T E
Sbjct: 125 LGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPE 184

Query: 139 MSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRAT 198
           M  R LIWVV ++ V V RY  WGD VE++TWV  +GKNGMRRDW+I D +T EI+TRA+
Sbjct: 185 MYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRAS 244

Query: 199 STWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLA 258
           S WV+MN++TR+LSKIPD+VR E+ P +++  + P  +DD  K+ KL +++AD IR GL 
Sbjct: 245 SVWVMMNQKTRKLSKIPDEVRREIEPHFVD--SAPVIEDDDRKLPKLDEKSADSIRKGLT 304

Query: 259 PRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTT 318
           PRWND+D NQHVNN KYIGWILES P  VLE  +L S+TLEYRREC + ++LESLT    
Sbjct: 305 PRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDP 364

Query: 319 AAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
           +     +            Q+ HLLR++ +  EIV+ RT+W  K
Sbjct: 365 SGEGYGS------------QFQHLLRLE-DGGEIVKGRTEWRPK 393

BLAST of Carg07613 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.3e-78
Identity = 146/287 (50.87%), Postives = 195/287 (67.94%), Query Frame = 0

Query: 76  DCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGA 135
           D L+  FV++   + Q+F IRSYEIG D+TA++ETLMN+ QET+LNH    GL  + FG 
Sbjct: 122 DVLVEPFVQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGR 181

Query: 136 TREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIIT 195
           T EM  R LIWVVT++ ++V RY  WGD +E+ TWV  +GKNGM RDW+I D H+ EI+ 
Sbjct: 182 TPEMCKRDLIWVVTKMQIEVNRYPTWGDTIEVTTWVSESGKNGMSRDWLISDCHSGEILI 241

Query: 196 RATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRS 255
           RATS W +MN++TRRLSKIPD+VR+E+ P++++  + P  +DD  K+ KL  +T D IR+
Sbjct: 242 RATSVWAMMNQKTRRLSKIPDEVRQEIVPYFVD--SAPVIEDDR-KLHKLDVKTGDSIRN 301

Query: 256 GLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTN 315
           GL PRWND D NQHVNNVKYI W+L+SVP  V E  +L  +TLEYRRECR+ ++LES+T 
Sbjct: 302 GLTPRWNDFDVNQHVNNVKYIAWLLKSVPTEVFETQELCGLTLEYRRECRRDSVLESVT- 361

Query: 316 TTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
                      A       +   Y HLLR++ N A+I   RT+W  K
Sbjct: 362 -----------AMDPSKEGDRSLYQHLLRLE-NGADIALGRTEWRPK 392

BLAST of Carg07613 vs. ExPASy TrEMBL
Match: A0A6J1E825 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111431501 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 5.1e-207
Identity = 363/366 (99.18%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLTNTTT   AAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTNTTT---AAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 363

BLAST of Carg07613 vs. ExPASy TrEMBL
Match: A0A6J1KFE2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111495310 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 2.8e-205
Identity = 358/366 (97.81%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MASIAASYLIPRTRRDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHIPGEKKGS 60
           MASIAASYLIPRTRR LTWGRNSSVVIA+AGGSRS LRSVDLVNGKKVNGVHIPGEKKGS
Sbjct: 1   MASIAASYLIPRTRRGLTWGRNSSVVIASAGGSRSGLRSVDLVNGKKVNGVHIPGEKKGS 60

Query: 61  LFMEEPHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120
           LFMEE HVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL
Sbjct: 61  LFMEETHVEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETAL 120

Query: 121 NHVTCSGLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180
           NHVTCSG+AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR
Sbjct: 121 NHVTCSGIAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMR 180

Query: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSD 240
           RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDK+DS+
Sbjct: 181 RDWIIRDYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKNDSE 240

Query: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300
           KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY
Sbjct: 241 KIDKLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEY 300

Query: 301 RRECRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360
           RRECRQSNLLESLT+TTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN
Sbjct: 301 RRECRQSNLLESLTSTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWN 360

Query: 361 SKPKRY 367
           SKPKRY
Sbjct: 361 SKPKRY 366

BLAST of Carg07613 vs. ExPASy TrEMBL
Match: A0A0A0LR84 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G051630 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.0e-162
Identity = 293/358 (81.84%), Postives = 314/358 (87.71%), Query Frame = 0

Query: 15  RDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHI-----PGEKKGSLFMEEP-HV 74
           R L   R +  ++   G   S+ RSVDLVNGKKVNGVH+      GEK+GSL +EE   +
Sbjct: 35  RGLNNRRCNFCIVMVRGRRSSSRRSVDLVNGKKVNGVHVEAPVFSGEKRGSLLVEESGAI 94

Query: 75  EGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 134
           EG KPLHDCLLGRFVE+ FVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL
Sbjct: 95  EGEKPLHDCLLGRFVEDKFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 154

Query: 135 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDY 194
           AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDY
Sbjct: 155 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDY 214

Query: 195 HTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDE 254
           HTRE+ITRATSTWVIMN+ETRRLSKIPDQVREELTPFYLNRIAIPTD++DS+KIDKLTD+
Sbjct: 215 HTREVITRATSTWVIMNKETRRLSKIPDQVREELTPFYLNRIAIPTDQNDSEKIDKLTDK 274

Query: 255 TADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSN 314
           TA+RIRSGLAPRWNDMDANQHVNNVKYIGWILESVPI VLED+DLTSMTLEYRRECRQSN
Sbjct: 275 TAERIRSGLAPRWNDMDANQHVNNVKYIGWILESVPITVLEDFDLTSMTLEYRRECRQSN 334

Query: 315 LLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKPKRY 367
           L+ESLT+TTT A    +          NL YTHLLRMQPNK EIVRARTQWNSKPK+Y
Sbjct: 335 LVESLTSTTTTATGDLS---------KNLHYTHLLRMQPNKDEIVRARTQWNSKPKQY 383

BLAST of Carg07613 vs. ExPASy TrEMBL
Match: A0A5A7UJM5 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004820 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 293/358 (81.84%), Postives = 311/358 (86.87%), Query Frame = 0

Query: 15  RDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHI-----PGEKKGSLFMEE-PHV 74
           R L   R +  ++   G   S  RSVDLVNGKKVNGVH+      GEK+GSL +EE    
Sbjct: 36  RGLNDRRCNFCIVMVRGRRSSPRRSVDLVNGKKVNGVHVEAPVFSGEKRGSLLVEENGAT 95

Query: 75  EGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 134
           EG KPLHDCLLGRFVE+ FVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL
Sbjct: 96  EGEKPLHDCLLGRFVEDRFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 155

Query: 135 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDY 194
           AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWV+AAGKNGMRRDWIIRDY
Sbjct: 156 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVNAAGKNGMRRDWIIRDY 215

Query: 195 HTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDE 254
           HTRE+ITRATSTWVIMN+ETRRLSKIPDQVREELTPFYLNRIAIPTD++D +KIDKLTDE
Sbjct: 216 HTREVITRATSTWVIMNKETRRLSKIPDQVREELTPFYLNRIAIPTDQNDVEKIDKLTDE 275

Query: 255 TADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSN 314
           TA+RIRSGLAPRWNDMDANQHVNNVKYIGWILESVPI+VLED+DLTSMTLEYRRECRQSN
Sbjct: 276 TAERIRSGLAPRWNDMDANQHVNNVKYIGWILESVPISVLEDFDLTSMTLEYRRECRQSN 335

Query: 315 LLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKPKRY 367
           L+ESLT TTTA A              NL YTHLLRMQPNK EIVRARTQWNSKPK+Y
Sbjct: 336 LVESLTTTTTATATGDRC--------KNLHYTHLLRMQPNKDEIVRARTQWNSKPKQY 385

BLAST of Carg07613 vs. ExPASy TrEMBL
Match: A0A1S3AZJ3 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103484573 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 293/358 (81.84%), Postives = 311/358 (86.87%), Query Frame = 0

Query: 15  RDLTWGRNSSVVIATAGGSRSALRSVDLVNGKKVNGVHI-----PGEKKGSLFMEE-PHV 74
           R L   R +  ++   G   S  RSVDLVNGKKVNGVH+      GEK+GSL +EE    
Sbjct: 36  RGLNDRRCNFCIVMVRGRRSSPRRSVDLVNGKKVNGVHVEAPVFSGEKRGSLLVEENGAT 95

Query: 75  EGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 134
           EG KPLHDCLLGRFVE+ FVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL
Sbjct: 96  EGEKPLHDCLLGRFVEDRFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGL 155

Query: 135 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDY 194
           AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWV+AAGKNGMRRDWIIRDY
Sbjct: 156 AGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVNAAGKNGMRRDWIIRDY 215

Query: 195 HTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDE 254
           HTRE+ITRATSTWVIMN+ETRRLSKIPDQVREELTPFYLNRIAIPTD++D +KIDKLTDE
Sbjct: 216 HTREVITRATSTWVIMNKETRRLSKIPDQVREELTPFYLNRIAIPTDQNDVEKIDKLTDE 275

Query: 255 TADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSN 314
           TA+RIRSGLAPRWNDMDANQHVNNVKYIGWILESVPI+VLED+DLTSMTLEYRRECRQSN
Sbjct: 276 TAERIRSGLAPRWNDMDANQHVNNVKYIGWILESVPISVLEDFDLTSMTLEYRRECRQSN 335

Query: 315 LLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKPKRY 367
           L+ESLT TTTA A              NL YTHLLRMQPNK EIVRARTQWNSKPK+Y
Sbjct: 336 LVESLTTTTTATATGDRC--------KNLHYTHLLRMQPNKDEIVRARTQWNSKPKQY 385

BLAST of Carg07613 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 322.0 bits (824), Expect = 6.1e-88
Identity = 158/284 (55.63%), Postives = 212/284 (74.65%), Query Frame = 0

Query: 79  LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSGLAGNDFGATRE 138
           +GR V++  V+ Q F IRSYEIG D++A++ET+MN LQETALNHV  +GL G+ FG+T E
Sbjct: 129 IGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVKTAGLLGDGFGSTPE 188

Query: 139 MSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRDYHTREIITRAT 198
           M  + LIWVVTR+ V V +Y  WGDVVE+DTWV  +GKNGMRRDW++RD +T E +TRA+
Sbjct: 189 MFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWLVRDCNTGETLTRAS 248

Query: 199 STWVIMNRETRRLSKIPDQVREELTPFYLNRIAIPTDKDDSDKIDKLTDETADRIRSGLA 258
           S WV+MN+ TRRLSKIP++VR E+ P+++N  + P   +DS K+ K+ D+TAD +RSGL 
Sbjct: 249 SVWVMMNKLTRRLSKIPEEVRGEIEPYFVN--SDPVLAEDSRKLTKIDDKTADYVRSGLT 308

Query: 259 PRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRECRQSNLLESLTNTTT 318
           PRW+D+D NQHVNNVKYIGWILES P+ ++E   L SMTLEYRREC + ++L+SLT  T 
Sbjct: 309 PRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTG 368

Query: 319 AAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSK 363
                 A+A        +++  HLLR+Q + AE+VR RT+W+SK
Sbjct: 369 CDIGNLATA-------GDVECQHLLRLQ-DGAEVVRGRTEWSSK 402

BLAST of Carg07613 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 261.9 bits (668), Expect = 7.6e-70
Identity = 136/307 (44.30%), Postives = 188/307 (61.24%), Query Frame = 0

Query: 68  VEGNKPLHDCLLGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCSG 127
           V G  P      GR +E+ F Y + FI+RSYE+G +KTAT+ET+ NLLQE A NHV   G
Sbjct: 62  VSGTSPADRLRFGRLMEDGFSYKEKFIVRSYEVGINKTATIETIANLLQEVACNHVQNVG 121

Query: 128 LAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIRD 187
            + + F  T  M    LIWV  R+H+++ +Y  W DVVEI+TW  + G+ G RRDWI++D
Sbjct: 122 FSTDGFATTLTMRKLHLIWVTARMHIEIYKYPAWSDVVEIETWCQSEGRIGTRRDWILKD 181

Query: 188 YHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFY--LNRIAIPTDKDDS-DKIDK 247
             T E+I RATS WV+MN++TRRL ++ D+VR+E   F     R+A P + + S  KI K
Sbjct: 182 CATGEVIGRATSKWVMMNQDTRRLQRVTDEVRDEYLVFCPPEPRLAFPEENNSSLKKIPK 241

Query: 248 LTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRREC 307
           L D  A     GL PR  D+D NQHVNNV YIGW+LES+P  +++ ++L  +TL+YRREC
Sbjct: 242 LED-PAQYSMLGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIIDTHELKVITLDYRREC 301

Query: 308 RQSNLLESLTNTTTAAAAAA------ASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQ 366
           +Q ++++SLT + T     +       S    +   N   + H+LR+  N  EI R RTQ
Sbjct: 302 QQDDIVDSLTTSETPNEVVSKLTGTNGSTTSSKREHNESHFLHILRLSENGQEINRGRTQ 361

BLAST of Carg07613 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 136/300 (45.33%), Postives = 186/300 (62.00%), Query Frame = 0

Query: 68  VEGNKPLHDCL-LGRFVEENFVYGQTFIIRSYEIGPDKTATMETLMNLLQETALNHVTCS 127
           V+G   L D L LG   E+   Y + F++RSYE+G +KTAT+ET+ NLLQE   NH    
Sbjct: 62  VQGLATLADQLRLGSLTEDGLSYKEKFVVRSYEVGSNKTATVETIANLLQEVGCNHAQSV 121

Query: 128 GLAGNDFGATREMSLRKLIWVVTRVHVQVQRYSCWGDVVEIDTWVDAAGKNGMRRDWIIR 187
           G + + F  T  M    LIWV  R+H+++ +Y  WGDVVEI+TW  + G+ G RRDWI++
Sbjct: 122 GFSTDGFATTTTMRKLHLIWVTARMHIEIYKYPAWGDVVEIETWCQSEGRIGTRRDWILK 181

Query: 188 DYHTREIITRATSTWVIMNRETRRLSKIPDQVREELTPFYLN--RIAIPTDKDDS-DKID 247
           D  T E+  RATS WV+MN++TRRL K+ D VR+E   F     R+A P + + S  KI 
Sbjct: 182 DSVTGEVTGRATSKWVMMNQDTRRLQKVSDDVRDEYLVFCPQEPRLAFPEENNRSLKKIP 241

Query: 248 KLTDETADRIRSGLAPRWNDMDANQHVNNVKYIGWILESVPINVLEDYDLTSMTLEYRRE 307
           KL D  A     GL PR  D+D NQHVNNV YIGW+LES+P  +++ ++L  +TL+YRRE
Sbjct: 242 KLED-PAQYSMIGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIVDTHELQVITLDYRRE 301

Query: 308 CRQSNLLESLTNTTTAAAAAAASAAIEEPPKNNLQYTHLLRMQPNKAEIVRARTQWNSKP 364
           C+Q ++++SLT TT+       SA       N+ Q+ HLLR+  +  EI R  T W  KP
Sbjct: 302 CQQDDVVDSLTTTTSEIGGTNGSATSGTQGHNDSQFLHLLRLSGDGQEINRGTTLWRKKP 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6584162.11.7e-209100.00Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
XP_023520196.12.1e-20798.91palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo ... [more]
XP_022923931.11.1e-20699.18palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita mosch... [more]
XP_023001057.15.8e-20597.81palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxim... [more]
XP_038895494.15.3e-16683.80palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispi... [more]
Match NameE-valueIdentityDescription
Q9SJE28.7e-8755.63Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia... [more]
Q9SQI35.3e-8453.87Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum... [more]
Q395139.9e-8354.58Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ... [more]
G3ESV02.9e-8253.87Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ... [more]
G3ESV13.3e-7850.87Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim... [more]
Match NameE-valueIdentityDescription
A0A6J1E8255.1e-20799.18Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111431... [more]
A0A6J1KFE22.8e-20597.81Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149531... [more]
A0A0A0LR841.0e-16281.84Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G051630... [more]
A0A5A7UJM51.5e-16181.84Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AZJ31.5e-16181.84Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103484573 PE... [more]
Match NameE-valueIdentityDescription
AT1G08510.16.1e-8855.63fatty acyl-ACP thioesterases B [more]
AT4G13050.17.6e-7044.30Acyl-ACP thioesterase [more]
AT3G25110.12.2e-6945.33fatA acyl-ACP thioesterase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 80..365
e-value: 2.3E-87
score: 294.5
NoneNo IPR availablePANTHERPTHR31727:SF5ACYL-[ACYL-CARRIER-PROTEIN] HYDROLASEcoord: 29..363
NoneNo IPR availableCDDcd005864HBTcoord: 92..205
e-value: 8.32589E-14
score: 64.9349
IPR002864Acyl-ACP thioesterasePFAMPF01643Acyl-ACP_TEcoord: 88..360
e-value: 7.3E-72
score: 241.6
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 29..363
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 254..359
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 83..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07613-RACarg07613-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016297 acyl-[acyl-carrier-protein] hydrolase activity
molecular_function GO:0000036 acyl carrier activity
molecular_function GO:0016790 thiolester hydrolase activity