Carg07556 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07556
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Aspartic proteinase
Location	Carg_Chr13: 7805265 .. 7809315 (-)
RNA-Seq Expression	Carg07556
Synteny	Carg07556

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGACTCTACAAGATTCGTCCGATTAAAAGACAACTCTGTCCCTCCAAAACGGACGGCACAGATCAAATCCTCTCTGCCATTTTCCAACGCTTGAGCTTGAATTGTAACCGGTTGATGAGTTGGGTCCGTTTGATTTCTTTTATTTTTGGAGTGGACAGGTCGGTTAACAGTCGCTTCTTCCTTCCAATGTATTCTTGCTTTGTTTATAAAGAGGCAGATTCGCGTATCATTTCCCCAATTCTCTTGTTCGTCTGAAATCTTTTTCTGGTGGGTTTTCTGATTTTTATGCCTCGATCGCCTTTTTTTAGTTGTTTTTGGTTTTGTTATTTGGACTTCATTGTTTACTTGATCCTCAAGTTGGTAAATGGGTTTTGTTTACTTTTTGAATTGTTGTTTGTTTATGTGATTATAGTTTTTTGTTTGTCTATCCGGATTCAGTTCATTGTTATCTGATTACTTTTTCGATGATCGTGGGGTTGTCGGGTTGCTGATCATGTTTTTGGGTCTTGAATTGTAAGCTTTTTGCATGTGTATGGGTTTGTTACGCCATTTGATCGCATCTTTTTAAGGGTTCTTTTTATTCTTCTGTTCTGCAATTTGGTGAAGTTCATAGTTTATACTTTATTGTTTAACCTCAATCTGGAACAATTCAAGAGGGTCACGCCTGAAATTTGATTTTGAATCATAGTTTTTCCATGTAAGAATTGAACAGTTTGGGGCTTGTTTGAGTAGGTTAGGTTTTTATTAATAGTTTAGGGCTCATCTATCTTGACTTGTATCAGGTTATAATTCATGATTTCTCAACTTCACTTTGGACTTGTAGGTTGCCAATGGCTTCGTACCACTCCAAAGCAGCTTTCTTGTGTTTGTTCTTGTTGGTTTCATTTAATATTGTGTGGTCTGCATCCAATGATGGGTTGCTTAGAGTTGGATTAAAGAAGATTAAGTTAGACCCAGAGAACCGTTTAGCTGCCCGCGTTGAGTCCAAGGATGCAGAGATTTTGAAAGCTGCTTTCAGAAAGTATAATCCTAAGGGTAATCTTGGAGAATCTTCTGATACTGATATTGTTGCGTTAAAGAACTACCTGGACGCTCAGTACTATGGTGAGATCGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTTGACACTGGCAGCTCCAATCTGTGGGTGCCTTCTGCCAAGTGCTTGTTTTCTGTAAGGATCTGTCGCCTTGATTATATTCAGAAACGTAAACCATTAAGTAGAAATGATACCTTTGGAATGTACTCAACTTTTTGGTTTTGTTGTAGGTGGCTTGTCATTTCCATGCCAGGTACAAGTCAAGCCGCTCAAGTTCATACAAGAAAAACGGTACTTTCTTAAGTCTATGTTCAAGATTGAATTGTAATTATTGGTACTTATTTTGATGTGGGGATGTTTGTAGTTTGTTCTTGCACGATGTCACGGAGCTGTTACTACAATTGCACTCGAAAATAATTCGGTTTATTGACATTGTGCAGGGACATCTGCTTCAATTCGGTATGGCACTGGAGCAGTCTCTGGTTTCTTTAGTTATGACAATGTCAAAGTTGGAGACCTAGTCGTAAAGGAACAGGTAACTTCCGGCATTAACTATGTTTATATCGAATTGAACAGTTGGAATATCAATGGTTTGTTCTTTATTGCTATTGTCCCTTTCAGGTGTTCATAGAAGCAACCAGAGAACCGAGTCTTACGTTTCTGGTGGCCAAGTTTGATGGGTTGTTGGGACTTGGTTTTCAAGAGATCGCTGTCGGTAATGCTGTCCCAGTATGGTAGGATATGTTCTTCCAACAATCCTGATTCTTGTTTAAGATTCCTCTTGGTTCATGGAGAGGCTTGAATGAGGCGTTTGTAATGTTCTTCTCAGGTATAACATGGTTGAACAAGGTCTGGTTAAGGAACCCGTCTTTTCGTTTTGGCTCAACCGCAACGTTGAGGAGGAAGAAGGAGGTGAAATTGTGTTTGGTGGGGTTGACCCTAAGCATTATAGGGGCAAGCACACTTATGTTCCTGTCACACAGAAAGGTTACTGGCAGGTTGGTTACCAAGTCTACATCTCATCCATTCTACTTCTAATTGTTATTACACCCCATGCTTATTTTTTCTTTCGGGTTTGTAGTTCGACATGGGCGATGTTCTCATTGATGGTGAACCAACTGGTATGCTTCCATGTTTCACTTATTAATTAAAGGCAAAGGAGAGTTTTTCTTTTTTTTTTTCTTTTGTTTCTGTAATAGGTGGTTTGAGTTCTGTGTTTATGTGCTTCTGACTACAGAAATTTGTTGATTCAGGATTTTGTGATGGTGGTTGTTCAGCAATAGCTGATTCTGGAACTTCACTGTTGGCTGGCCCAACTGTGCGTACATAGACTTGACCTTGTTCTTCCCTGTCATCTGTAACTTGGCTTCACATGTGCTATATACCATATTGCATGACACTTTTTTATAAATAAGTTGAGACTTCTCCGTTTTAAGATGGCTAATCAACCATTCGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAACATTCTTTAGAAGGGTGTGGAAACCTCTCCCTGCTAGCGGTGGGCTTGAGCTTGAGCTTGTTATAATCAGTGTCTTTATAATCCTTGGACGTAAAGACCGGTTAGCCTTTGATGCAAGAACATACTTGATTGAATAGAAATACAGTGATTGTCTCTGCAGTTGGGTTAAATGGCTATTCACTCCAATGTTTGTCTCTCTCTTTCAGCCTGTTATAACTATGATCAATCACGCCATTGGAGCTAAAGGAGTTGTTAGCCAGCAATGCAAGGCCGTTGTCGCACAATATGGGCAAACTATTATGGACTTGCTTTTATCTGAGGCAAGATAACGTTTTCGTTTTTAACTTGTTGATTCTTAACGTAGCATTTTGGTCTTATACTGCTTAAGATACCCAATCTTTATACCTTTTATGGAGCTCTTTTGATTTTTTCAACAGGCAGATCCGAAGAAGATATGTTCTCAAATTAATTTGTGTACTTTCGATGGAACTCGAGGAGTCAGGTGAGTGAGGCATACATGCTTTTTATCGGACCACACACGTTTACTCGGTGAATTTACTGACCCTGAAATTATGTTGGTCATGTAGTATGGGAATTGAGAGTGTGGTAGACGAGAATGCCGGGAAATCATCCGACAGTCTACGCGACGGTATGTGCTCTGTATGTGAGATGACTGTTGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACCAAAGAACGCATAATAAACTATATCAATGAGGTAAAAAAGATCGGTTTTCTTCAGAATATCGATTCTTGTTGACTCGACTAATTAACTCCCCTGCAGCTATGTGATCGTATGCCGAGTCCAATGGGACAATCAGCCGTCGACTGTGGACAACTTTCTTCCATGCCTACTGTTTCCTTCACCATTGGTGGCAAAATATTCGACCTTGCACCCGAAGAGGTAAATACAGAAAAGAAAATCGCTTGAACGGAGAATTTTATCCCCTTTCGGTTTTTATCCCTTTTCGGTATCGTTCTCTAAAATAGCGAGCAATGTGAACAGTATATACTCAAGGTTGGTGAGGGTCCTGTAGCTCAGTGCATAAGTGGATTTACTGCATTCGATATCCCTCCTCCTCGTGGACCCCTTTGGTATATATGTTTTTACCATTTACTTTGCTTTCAGAATCACTCAAAGTATCCTTCCCTTTCATTAAGAACCTGTTTTCATTGGCCACAGGATCTTGGGAGATGTCTTCATGGGTCGCTACCACACCGTCTTTGATTTTGGCAAGCTGAGAGTCGGTTTTGCAGAGGCAGCTTGAAGAAACCTCCTTGTTTGGTATCTTTTAATGTTTATACATATGAGCCATCTTTAGTTTTCAGTCGAACCTTACTTTAAGTTGATGGATTTATAAAGTAAAGCCCAAGATTGTAAATGCTTGTTACTGTTCTAATCTTCTATATAGATACACAGCTTGTACAGTTGCTTTATATCTCTGCTGATTTATACATGAACTTTTAG

mRNA sequence

ACGACTCTACAAGATTCGTCCGATTAAAAGACAACTCTGTCCCTCCAAAACGGACGGCACAGATCAAATCCTCTCTGCCATTTTCCAACGCTTGAGCTTGAATTGTAACCGGTTGATGAGTTGGGTCCGTTTGATTTCTTTTATTTTTGGAGTGGACAGGTCGGTTAACAGTCGCTTCTTCCTTCCAATGTATTCTTGCTTTGTTTATAAAGAGGCAGATTCGCGTATCATTTCCCCAATTCTCTTGTTCGTCTGAAATCTTTTTCTGGTTGCCAATGGCTTCGTACCACTCCAAAGCAGCTTTCTTGTGTTTGTTCTTGTTGGTTTCATTTAATATTGTGTGGTCTGCATCCAATGATGGGTTGCTTAGAGTTGGATTAAAGAAGATTAAGTTAGACCCAGAGAACCGTTTAGCTGCCCGCGTTGAGTCCAAGGATGCAGAGATTTTGAAAGCTGCTTTCAGAAAGTATAATCCTAAGGGTAATCTTGGAGAATCTTCTGATACTGATATTGTTGCGTTAAAGAACTACCTGGACGCTCAGTACTATGGTGAGATCGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTTGACACTGGCAGCTCCAATCTGTGGGTGCCTTCTGCCAAGTGCTTGTTTTCTGTGGCTTGTCATTTCCATGCCAGGTACAAGTCAAGCCGCTCAAGTTCATACAAGAAAAACGGGACATCTGCTTCAATTCGGTATGGCACTGGAGCAGTCTCTGGTTTCTTTAGTTATGACAATGTCAAAGTTGGAGACCTAGTCGTAAAGGAACAGGTGTTCATAGAAGCAACCAGAGAACCGAGTCTTACGTTTCTGGTGGCCAAGTTTGATGGGTTGTTGGGACTTGGTTTTCAAGAGATCGCTGTCGGTAATGCTGTCCCAGTATGGTATAACATGGTTGAACAAGGTCTGGTTAAGGAACCCGTCTTTTCGTTTTGGCTCAACCGCAACGTTGAGGAGGAAGAAGGAGGTGAAATTGTGTTTGGTGGGGTTGACCCTAAGCATTATAGGGGCAAGCACACTTATGTTCCTGTCACACAGAAAGGTTACTGGCAGTTCGACATGGGCGATGTTCTCATTGATGGTGAACCAACTGGATTTTGTGATGGTGGTTGTTCAGCAATAGCTGATTCTGGAACTTCACTGTTGGCTGGCCCAACTCCTGTTATAACTATGATCAATCACGCCATTGGAGCTAAAGGAGTTGTTAGCCAGCAATGCAAGGCCGTTGTCGCACAATATGGGCAAACTATTATGGACTTGCTTTTATCTGAGGCAGATCCGAAGAAGATATGTTCTCAAATTAATTTGTGTACTTTCGATGGAACTCGAGGAGTCAGTATGGGAATTGAGAGTGTGGTAGACGAGAATGCCGGGAAATCATCCGACAGTCTACGCGACGGTATGTGCTCTGTATGTGAGATGACTGTTGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACCAAAGAACGCATAATAAACTATATCAATGAGCTATGTGATCGTATGCCGAGTCCAATGGGACAATCAGCCGTCGACTGTGGACAACTTTCTTCCATGCCTACTGTTTCCTTCACCATTGGTGGCAAAATATTCGACCTTGCACCCGAAGAGTATATACTCAAGGTTGGTGAGGGTCCTGTAGCTCAGTGCATAAGTGGATTTACTGCATTCGATATCCCTCCTCCTCGTGGACCCCTTTGGATCTTGGGAGATGTCTTCATGGGTCGCTACCACACCGTCTTTGATTTTGGCAAGCTGAGAGTCGGTTTTGCAGAGGCAGCTTGAAGAAACCTCCTTGTTTGGTATCTTTTAATGTTTATACATATGAGCCATCTTTAGTTTTCAGTCGAACCTTACTTTAAGTTGATGGATTTATAAAGTAAAGCCCAAGATTGTAAATGCTTGTTACTGTTCTAATCTTCTATATAGATACACAGCTTGTACAGTTGCTTTATATCTCTGCTGATTTATACATGAACTTTTAG

Coding sequence (CDS)

ATGGCTTCGTACCACTCCAAAGCAGCTTTCTTGTGTTTGTTCTTGTTGGTTTCATTTAATATTGTGTGGTCTGCATCCAATGATGGGTTGCTTAGAGTTGGATTAAAGAAGATTAAGTTAGACCCAGAGAACCGTTTAGCTGCCCGCGTTGAGTCCAAGGATGCAGAGATTTTGAAAGCTGCTTTCAGAAAGTATAATCCTAAGGGTAATCTTGGAGAATCTTCTGATACTGATATTGTTGCGTTAAAGAACTACCTGGACGCTCAGTACTATGGTGAGATCGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTTGACACTGGCAGCTCCAATCTGTGGGTGCCTTCTGCCAAGTGCTTGTTTTCTGTGGCTTGTCATTTCCATGCCAGGTACAAGTCAAGCCGCTCAAGTTCATACAAGAAAAACGGGACATCTGCTTCAATTCGGTATGGCACTGGAGCAGTCTCTGGTTTCTTTAGTTATGACAATGTCAAAGTTGGAGACCTAGTCGTAAAGGAACAGGTGTTCATAGAAGCAACCAGAGAACCGAGTCTTACGTTTCTGGTGGCCAAGTTTGATGGGTTGTTGGGACTTGGTTTTCAAGAGATCGCTGTCGGTAATGCTGTCCCAGTATGGTATAACATGGTTGAACAAGGTCTGGTTAAGGAACCCGTCTTTTCGTTTTGGCTCAACCGCAACGTTGAGGAGGAAGAAGGAGGTGAAATTGTGTTTGGTGGGGTTGACCCTAAGCATTATAGGGGCAAGCACACTTATGTTCCTGTCACACAGAAAGGTTACTGGCAGTTCGACATGGGCGATGTTCTCATTGATGGTGAACCAACTGGATTTTGTGATGGTGGTTGTTCAGCAATAGCTGATTCTGGAACTTCACTGTTGGCTGGCCCAACTCCTGTTATAACTATGATCAATCACGCCATTGGAGCTAAAGGAGTTGTTAGCCAGCAATGCAAGGCCGTTGTCGCACAATATGGGCAAACTATTATGGACTTGCTTTTATCTGAGGCAGATCCGAAGAAGATATGTTCTCAAATTAATTTGTGTACTTTCGATGGAACTCGAGGAGTCAGTATGGGAATTGAGAGTGTGGTAGACGAGAATGCCGGGAAATCATCCGACAGTCTACGCGACGGTATGTGCTCTGTATGTGAGATGACTGTTGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACCAAAGAACGCATAATAAACTATATCAATGAGCTATGTGATCGTATGCCGAGTCCAATGGGACAATCAGCCGTCGACTGTGGACAACTTTCTTCCATGCCTACTGTTTCCTTCACCATTGGTGGCAAAATATTCGACCTTGCACCCGAAGAGTATATACTCAAGGTTGGTGAGGGTCCTGTAGCTCAGTGCATAAGTGGATTTACTGCATTCGATATCCCTCCTCCTCGTGGACCCCTTTGGATCTTGGGAGATGTCTTCATGGGTCGCTACCACACCGTCTTTGATTTTGGCAAGCTGAGAGTCGGTTTTGCAGAGGCAGCTTGA

Protein sequence

MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA

Homology

[Close]

BLAST of Carg07556 vs. NCBI nr
Match: XP_022923995.1 (aspartic proteinase isoform X3 [Cucurbita moschata] >KAG7019698.1 hypothetical protein SDJN02_18661 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.3e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 514

[Close]

BLAST of Carg07556 vs. NCBI nr
Match: XP_022923993.1 (aspartic proteinase isoform X1 [Cucurbita moschata])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.3e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 73  MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 132

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 133 AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 192

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 193 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 252

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 253 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 312

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 313 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 372

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 373 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 432

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 433 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 492

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 493 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 552

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 553 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 586

[Close]

BLAST of Carg07556 vs. NCBI nr
Match: KAG6584099.1 (hypothetical protein SDJN03_20031, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.3e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 78  MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 137

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 138 AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 197

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 198 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 257

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 258 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 317

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 318 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 377

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 378 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 437

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 438 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 497

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 498 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 557

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 558 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 591

[Close]

BLAST of Carg07556 vs. NCBI nr
Match: XP_022923994.1 (aspartic proteinase isoform X2 [Cucurbita moschata])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.3e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 27  MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 86

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 87  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 146

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 147 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 206

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 207 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 266

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 267 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 326

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 327 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 386

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 387 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 446

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 447 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 506

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 507 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 540

[Close]

BLAST of Carg07556 vs. NCBI nr
Match: XP_023001251.1 (aspartic proteinase isoform X2 [Cucurbita maxima])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.5e-299
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIV SASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 1   MASYHSKAAFLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSL DGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 361 TRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 514

[Close]

BLAST of Carg07556 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 1.4e-297
Identity = 507/514 (98.64%), Postives = 508/514 (98.83%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIV SASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 1   MASYHSKAAFLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV   +
Sbjct: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCE 120

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSL DGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 361 TRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVG AEAA
Sbjct: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513

[Close]

BLAST of Carg07556 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.2e-230
Identity = 383/503 (76.14%), Postives = 435/503 (86.48%), Query Frame = 0


Query: 15  LLVSFNIVWSA---SNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNL 74
           L+VSF + +SA    NDG  RVGLKK+KLD +NRLAARVESK  + L+A          L
Sbjct: 12  LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRAY--------RL 71

Query: 75  GESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSVACHFHA 134
           G+S D D+V LKNYLDAQYYGEIAIGTPPQKFTV+FDTGSSNLWVPS+KC FS+AC  H 
Sbjct: 72  GDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHP 131

Query: 135 RYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFLV 194
           +YKSSRSS+Y+KNG +A+I YGTGA++GFFS D V VGDLVVK+Q FIEAT+EP +TF+V
Sbjct: 132 KYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVV 191

Query: 195 AKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGVDP 254
           AKFDG+LGLGFQEI+VG A PVWYNM++QGL+KEPVFSFWLNRN +EEEGGE+VFGGVDP
Sbjct: 192 AKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFGGVDP 251

Query: 255 KHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVITMI 314
            H++GKHTYVPVTQKGYWQFDMGDVLI G PTGFC+ GCSAIADSGTSLLAGPT +ITMI
Sbjct: 252 NHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITMI 311

Query: 315 NHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESV 374
           NHAIGA GVVSQQCK VV QYGQTI+DLLLSE  PKKICSQI LCTFDGTRGVSMGIESV
Sbjct: 312 NHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIESV 371

Query: 375 VDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAV 434
           VD+   K S+ + D  CS CEM VVW+Q+QLRQN T+ERI+NY+NELC+R+PSPMG+SAV
Sbjct: 372 VDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAV 431

Query: 435 DCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGD 494
           DC QLS+MPTVS TIGGK+FDLAPEEY+LKVGEGPVAQCISGF A D+ PPRGPLWILGD
Sbjct: 432 DCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGD 491

Query: 495 VFMGRYHTVFDFGKLRVGFAEAA 515
           VFMG+YHTVFDFG  +VGFAEAA
Sbjct: 492 VFMGKYHTVFDFGNEQVGFAEAA 506

[Close]

BLAST of Carg07556 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 4.4e-230
Identity = 384/514 (74.71%), Postives = 431/514 (83.85%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           M  Y    AF      + F   +S  NDG  RVGLKK+KLDP NRLA R  SK  E L++
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  AFRKYNPKGNL-GESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSA 120
           + R YN   NL G+S D DIV LKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS 
Sbjct: 61  SLRSYN--NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSG 120

Query: 121 KCLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFI 180
           KC FS++C+FHA+YKSSRSS+YKK+G  A+I YG+G++SGFFSYD V VGDLVVK+Q FI
Sbjct: 121 KCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFI 180

Query: 181 EATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEE 240
           E T EP LTFLVAKFDGLLGLGFQEIAVGNA PVWYNM++QGL+K PVFSFWLNR+ + E
Sbjct: 181 ETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSE 240

Query: 241 EGGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTS 300
           EGGEIVFGGVDPKH+RG+HT+VPVTQ+GYWQFDMG+VLI GE TG+C  GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFD 360
           LLAGPT V+ MIN AIGA GVVSQQCK VV QYGQTI+DLLL+E  PKKICSQI LC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELC 420
           GT GVSMGIESVVD+   +SS  LRD  C  CEM VVW+Q+QLRQN T+ERI+NYINE+C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 DRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDI 480
           +RMPSP G+SAVDC QLS MPTVSFTIGGK+FDLAPEEY+LK+GEGPVAQCISGFTA DI
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEA 514
           PPPRGPLWILGDVFMG+YHTVFDFG  +VGFAEA
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of Carg07556 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 727.2 bits (1876), Expect = 1.3e-208
Identity = 343/491 (69.86%), Postives = 411/491 (83.71%), Query Frame = 0


Query: 24  SASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNLGESSDTDIVALK 83
           +++ +GL+R+ LKK  +D  +R+AAR+  ++    +   R  N  G  G   + DIVALK
Sbjct: 22  ASAAEGLVRIALKKRPIDENSRVAARLSGEEG-ARRLGLRGANSLG--GGGGEGDIVALK 81

Query: 84  NYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSVACHFHARYKSSRSSSYKK 143
           NY++AQY+GEI +GTPPQKFTVIFDTGSSNLWVPSAKC FS+AC FH+RYKS +SS+Y+K
Sbjct: 82  NYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACFFHSRYKSGQSSTYQK 141

Query: 144 NGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFLVAKFDGLLGLGFQ 203
           NG  A+I+YGTG+++GFFS D+V VGDLVVK+Q FIEAT+EP LTF+VAKFDG+LGLGFQ
Sbjct: 142 NGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKEPGLTFMVAKFDGILGLGFQ 201

Query: 204 EIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGVDPKHYRGKHTYVPV 263
           EI+VG+AVPVWY MVEQGLV EPVFSFW NR+ +E EGGEIVFGG+DP HY+G HTYVPV
Sbjct: 202 EISVGDAVPVWYKMVEQGLVSEPVFSFWFNRHSDEGEGGEIVFGGMDPSHYKGNHTYVPV 261

Query: 264 TQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVITMINHAIGAKGVVSQ 323
           +QKGYWQF+MGDVLI G+ TGFC  GCSAIADSGTSLLAGPT +IT IN  IGA GVVSQ
Sbjct: 262 SQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLAGPTAIITEINEKIGATGVVSQ 321

Query: 324 QCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESVVDENAGKSSDSL 383
           +CK VV+QYGQ I+DLLL+E  P KICSQ+ LCTFDG  GVS GI+SVVD+ AG+S+   
Sbjct: 322 ECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSAGIKSVVDDEAGESNGLQ 381

Query: 384 RDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVS 443
              MC+ CEM VVWMQNQL QN+T++ I+NYIN+LCD++PSPMG+S+VDCG L+SMP +S
Sbjct: 382 SGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEIS 441

Query: 444 FTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDF 503
           FTIGGK F L PEEYILKVGEG  AQCISGFTA DIPPPRGPLWILGDVFMG YHTVFD+
Sbjct: 442 FTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDY 501

Query: 504 GKLRVGFAEAA 515
           GK+RVGFA++A
Sbjct: 502 GKMRVGFAKSA 509

[Close]

BLAST of Carg07556 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.6e-208
Identity = 345/506 (68.18%), Postives = 415/506 (82.02%), Query Frame = 0


Query: 9   AFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPK 68
           A L   LL+   +  ++  +GL+R+ LKK  +D  +R+A  +   + + L +     NP 
Sbjct: 9   ALLAAVLLLQTVLPAASEAEGLVRIALKKRPIDRNSRVATGLSGGEEQPLLSG---ANP- 68

Query: 69  GNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSVACH 128
             L    + DIVALKNY++AQY+GEI +GTPPQKFTVIFDTGSSNLWVPSAKC FS+AC+
Sbjct: 69  --LRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACY 128

Query: 129 FHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLT 188
            H+RYK+  SS+YKKNG  A+I+YGTG+++G+FS D+V VGDLVVK+Q FIEAT+EP +T
Sbjct: 129 LHSRYKAGASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEATKEPGIT 188

Query: 189 FLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGG 248
           FLVAKFDG+LGLGF+EI+VG AVPVWY M+EQGLV +PVFSFWLNR+V+E EGGEI+FGG
Sbjct: 189 FLVAKFDGILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHVDEGEGGEIIFGG 248

Query: 249 VDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVI 308
           +DPKHY G+HTYVPVTQKGYWQFDMGDVL+ G+ TGFC GGC+AIADSGTSLLAGPT +I
Sbjct: 249 MDPKHYVGEHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSLLAGPTAII 308

Query: 309 TMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGI 368
           T IN  IGA GVVSQ+CK +V+QYGQ I+DLLL+E  PKKICSQ+ LCTFDGTRGVS GI
Sbjct: 309 TEINEKIGAAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDGTRGVSAGI 368

Query: 369 ESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQ 428
            SVVD+   KS+    D MCS CEM VVWMQNQL QN+T++ I++Y+N+LC+R+PSPMG+
Sbjct: 369 RSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGE 428

Query: 429 SAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWI 488
           SAVDCG L SMP + FTIGGK F L PEEYILKVGEG  AQCISGFTA DIPPPRGPLWI
Sbjct: 429 SAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWI 488

Query: 489 LGDVFMGRYHTVFDFGKLRVGFAEAA 515
           LGDVFMG YHTVFD+GKLR+GFA+AA
Sbjct: 489 LGDVFMGPYHTVFDYGKLRIGFAKAA 508

[Close]

BLAST of Carg07556 vs. ExPASy TrEMBL
Match: A0A6J1EB32 (aspartic proteinase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.5e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 73  MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 132

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 133 AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 192

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 193 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 252

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 253 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 312

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 313 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 372

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 373 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 432

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 433 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 492

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 493 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 552

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 553 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 586

[Close]

BLAST of Carg07556 vs. ExPASy TrEMBL
Match: A0A6J1E7W9 (aspartic proteinase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.5e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 27  MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 86

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 87  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 146

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 147 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 206

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 207 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 266

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 267 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 326

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 327 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 386

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 387 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 446

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 447 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 506

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 507 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 540

[Close]

BLAST of Carg07556 vs. ExPASy TrEMBL
Match: A0A6J1EDJ1 (aspartic proteinase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.5e-302
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 514

[Close]

BLAST of Carg07556 vs. ExPASy TrEMBL
Match: A0A6J1KI39 (aspartic proteinase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495439 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 7.2e-300
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIV SASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 1   MASYHSKAAFLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSL DGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 361 TRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 514

[Close]

BLAST of Carg07556 vs. ExPASy TrEMBL
Match: A0A6J1KM78 (aspartic proteinase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495439 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 7.2e-300
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           MASYHSKAAFLCLFLLVSFNIV SASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA
Sbjct: 4   MASYHSKAAFLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 63

Query: 61  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 120
           AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK
Sbjct: 64  AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 123

Query: 121 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180
           CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE
Sbjct: 124 CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 183

Query: 181 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240
           ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE
Sbjct: 184 ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 243

Query: 241 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300
           GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL
Sbjct: 244 GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 303

Query: 301 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360
           LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG
Sbjct: 304 LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 363

Query: 361 TRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420
           TRGVSMGIESVVDENAGKSSDSL DGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD
Sbjct: 364 TRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 423

Query: 421 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480
           RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP
Sbjct: 424 RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 483

Query: 481 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 484 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 517

[Close]

BLAST of Carg07556 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 800.4 bits (2066), Expect = 8.3e-232
Identity = 383/503 (76.14%), Postives = 435/503 (86.48%), Query Frame = 0


Query: 15  LLVSFNIVWSA---SNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNL 74
           L+VSF + +SA    NDG  RVGLKK+KLD +NRLAARVESK  + L+A          L
Sbjct: 12  LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRAY--------RL 71

Query: 75  GESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSVACHFHA 134
           G+S D D+V LKNYLDAQYYGEIAIGTPPQKFTV+FDTGSSNLWVPS+KC FS+AC  H 
Sbjct: 72  GDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHP 131

Query: 135 RYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFLV 194
           +YKSSRSS+Y+KNG +A+I YGTGA++GFFS D V VGDLVVK+Q FIEAT+EP +TF+V
Sbjct: 132 KYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVV 191

Query: 195 AKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGVDP 254
           AKFDG+LGLGFQEI+VG A PVWYNM++QGL+KEPVFSFWLNRN +EEEGGE+VFGGVDP
Sbjct: 192 AKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFGGVDP 251

Query: 255 KHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVITMI 314
            H++GKHTYVPVTQKGYWQFDMGDVLI G PTGFC+ GCSAIADSGTSLLAGPT +ITMI
Sbjct: 252 NHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITMI 311

Query: 315 NHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESV 374
           NHAIGA GVVSQQCK VV QYGQTI+DLLLSE  PKKICSQI LCTFDGTRGVSMGIESV
Sbjct: 312 NHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIESV 371

Query: 375 VDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAV 434
           VD+   K S+ + D  CS CEM VVW+Q+QLRQN T+ERI+NY+NELC+R+PSPMG+SAV
Sbjct: 372 VDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAV 431

Query: 435 DCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGD 494
           DC QLS+MPTVS TIGGK+FDLAPEEY+LKVGEGPVAQCISGF A D+ PPRGPLWILGD
Sbjct: 432 DCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGD 491

Query: 495 VFMGRYHTVFDFGKLRVGFAEAA 515
           VFMG+YHTVFDFG  +VGFAEAA
Sbjct: 492 VFMGKYHTVFDFGNEQVGFAEAA 506

[Close]

BLAST of Carg07556 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 3.1e-231
Identity = 384/514 (74.71%), Postives = 431/514 (83.85%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           M  Y    AF      + F   +S  NDG  RVGLKK+KLDP NRLA R  SK  E L++
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  AFRKYNPKGNL-GESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSA 120
           + R YN   NL G+S D DIV LKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS 
Sbjct: 61  SLRSYN--NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSG 120

Query: 121 KCLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFI 180
           KC FS++C+FHA+YKSSRSS+YKK+G  A+I YG+G++SGFFSYD V VGDLVVK+Q FI
Sbjct: 121 KCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFI 180

Query: 181 EATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEE 240
           E T EP LTFLVAKFDGLLGLGFQEIAVGNA PVWYNM++QGL+K PVFSFWLNR+ + E
Sbjct: 181 ETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSE 240

Query: 241 EGGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTS 300
           EGGEIVFGGVDPKH+RG+HT+VPVTQ+GYWQFDMG+VLI GE TG+C  GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFD 360
           LLAGPT V+ MIN AIGA GVVSQQCK VV QYGQTI+DLLL+E  PKKICSQI LC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELC 420
           GT GVSMGIESVVD+   +SS  LRD  C  CEM VVW+Q+QLRQN T+ERI+NYINE+C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 DRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDI 480
           +RMPSP G+SAVDC QLS MPTVSFTIGGK+FDLAPEEY+LK+GEGPVAQCISGFTA DI
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEA 514
           PPPRGPLWILGDVFMG+YHTVFDFG  +VGFAEA
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of Carg07556 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 3.1e-231
Identity = 384/514 (74.71%), Postives = 431/514 (83.85%), Query Frame = 0


Query: 1   MASYHSKAAFLCLFLLVSFNIVWSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60
           M  Y    AF      + F   +S  NDG  RVGLKK+KLDP NRLA R  SK  E L++
Sbjct: 1   MGVYSRAVAFSVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRS 60

Query: 61  AFRKYNPKGNL-GESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSA 120
           + R YN   NL G+S D DIV LKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS 
Sbjct: 61  SLRSYN--NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSG 120

Query: 121 KCLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFI 180
           KC FS++C+FHA+YKSSRSS+YKK+G  A+I YG+G++SGFFSYD V VGDLVVK+Q FI
Sbjct: 121 KCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFI 180

Query: 181 EATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEE 240
           E T EP LTFLVAKFDGLLGLGFQEIAVGNA PVWYNM++QGL+K PVFSFWLNR+ + E
Sbjct: 181 ETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSE 240

Query: 241 EGGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTS 300
           EGGEIVFGGVDPKH+RG+HT+VPVTQ+GYWQFDMG+VLI GE TG+C  GCSAIADSGTS
Sbjct: 241 EGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTS 300

Query: 301 LLAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFD 360
           LLAGPT V+ MIN AIGA GVVSQQCK VV QYGQTI+DLLL+E  PKKICSQI LC +D
Sbjct: 301 LLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYD 360

Query: 361 GTRGVSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELC 420
           GT GVSMGIESVVD+   +SS  LRD  C  CEM VVW+Q+QLRQN T+ERI+NYINE+C
Sbjct: 361 GTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEIC 420

Query: 421 DRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDI 480
           +RMPSP G+SAVDC QLS MPTVSFTIGGK+FDLAPEEY+LK+GEGPVAQCISGFTA DI
Sbjct: 421 ERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDI 480

Query: 481 PPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEA 514
           PPPRGPLWILGDVFMG+YHTVFDFG  +VGFAEA
Sbjct: 481 PPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

[Close]

BLAST of Carg07556 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 704.5 bits (1817), Expect = 6.2e-203
Identity = 337/511 (65.95%), Postives = 417/511 (81.60%), Query Frame = 0


Query: 9   AFLCLFLLVSFNIVWSAS----NDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRK 68
           +FL +FLL    ++ +AS     DG +R+GLKK KLD  NRLA+++       LK     
Sbjct: 7   SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQL------FLKNRGSH 66

Query: 69  YNPKGNLG-ESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLF 128
           ++PK        + D+V LKNYLDAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67  WSPKHYFRLNDENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 129 SVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATR 188
           SVAC+FH++YK+S+SSSY+KNG  ASIRYGTGA+SG+FS D+VKVGD+VVKEQ FIEAT 
Sbjct: 127 SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 189 EPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGE 248
           EP +TFL+AKFDG+LGLGF+EI+VGN+ PVWYNMVE+GLVKEP+FSFWLNRN ++ EGGE
Sbjct: 187 EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 249 IVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAG 308
           IVFGGVDPKH++G+HT+VPVT KGYWQFDMGD+ I G+PTG+C  GCSAIADSGTSLL G
Sbjct: 247 IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 309 PTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRG 368
           P+ VITMINHAIGA+G+VS++CKAVV QYG+T+++ LL++ DPKK+CSQI +C +DGT+ 
Sbjct: 307 PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGVCAYDGTQS 366

Query: 369 VSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMP 428
           VSMGI+SVVD+    +S  L   MCS CEM  VWM+++L QNQT+ERI+ Y  ELCD +P
Sbjct: 367 VSMGIQSVVDDG---TSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 429 SPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPR 488
           +   QSAVDCG++SSMP V+F+IGG+ FDL P++YI K+GEG  +QC SGFTA DI PPR
Sbjct: 427 TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 489 GPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           GPLWILGD+FMG YHTVFD+GK RVGFA+AA
Sbjct: 487 GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

[Close]

BLAST of Carg07556 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 691.8 bits (1784), Expect = 4.1e-199
Identity = 334/511 (65.36%), Postives = 414/511 (81.02%), Query Frame = 0


Query: 9   AFLCLFLLVSFNIVWSAS----NDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRK 68
           +FL +FLL    ++ +AS     DG +R+GLKK KLD  NRLA+++       LK     
Sbjct: 7   SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQL------FLKNRGSH 66

Query: 69  YNPKGNLG-ESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLF 128
           ++PK        + D+V LKNYLDAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67  WSPKHYFRLNDENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 129 SVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATR 188
           SVAC+FH++YK+S+SSSY+KNG  ASIRYGTGA+SG+FS D+VKVGD+VVKEQ FIEAT 
Sbjct: 127 SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 189 EPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGE 248
           EP +TFL+AKFDG+LGLGF+EI+VGN+ PVWYNMVE+GLVKEP+FSFWLNRN ++ EGGE
Sbjct: 187 EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 249 IVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAG 308
           IVFGGVDPKH++G+HT+VPVT KGYWQFDMGD+ I G+PTG+C  GCSAIADSGTSLL G
Sbjct: 247 IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 309 PTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRG 368
           P+ VITMINHAIGA+G+VS++CKAVV QYG+T+++ LL++    K+CSQI +C +DGT+ 
Sbjct: 307 PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQ----KVCSQIGVCAYDGTQS 366

Query: 369 VSMGIESVVDENAGKSSDSLRDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMP 428
           VSMGI+SVVD+    +S  L   MCS CEM  VWM+++L QNQT+ERI+ Y  ELCD +P
Sbjct: 367 VSMGIQSVVDDG---TSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 429 SPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPR 488
           +   QSAVDCG++SSMP V+F+IGG+ FDL P++YI K+GEG  +QC SGFTA DI PPR
Sbjct: 427 TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 489 GPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 515
           GPLWILGD+FMG YHTVFD+GK RVGFA+AA
Sbjct: 487 GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

The following BLAST results are available for this feature:

BLAST of Carg07556 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923995.1	9.3e-302	100.00	aspartic proteinase isoform X3 [Cucurbita moschata] >KAG7019698.1 hypothetical p...	[more]
XP_022923993.1	9.3e-302	100.00	aspartic proteinase isoform X1 [Cucurbita moschata]	[more]
KAG6584099.1	9.3e-302	100.00	hypothetical protein SDJN03_20031, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022923994.1	9.3e-302	100.00	aspartic proteinase isoform X2 [Cucurbita moschata]	[more]
XP_023001251.1	1.5e-299	99.61	aspartic proteinase isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg07556 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04057	1.4e-297	98.64	Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
O65390	1.2e-230	76.14	Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1	[more]
Q8VYL3	4.4e-230	74.71	Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1	[more]
Q42456	1.3e-208	69.86	Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g...	[more]
P42210	3.6e-208	68.18	Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]

BLAST of Carg07556 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EB32	4.5e-302	100.00	aspartic proteinase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=...	[more]
A0A6J1E7W9	4.5e-302	100.00	aspartic proteinase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=...	[more]
A0A6J1EDJ1	4.5e-302	100.00	aspartic proteinase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431547 PE=...	[more]
A0A6J1KI39	7.2e-300	99.61	aspartic proteinase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495439 PE=3 ...	[more]
A0A6J1KM78	7.2e-300	99.61	aspartic proteinase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495439 PE=3 ...	[more]

BLAST of Carg07556 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11910.1	8.3e-232	76.14	aspartic proteinase A1	[more]
AT1G62290.1	3.1e-231	74.71	Saposin-like aspartyl protease family protein	[more]
AT1G62290.2	3.1e-231	74.71	Saposin-like aspartyl protease family protein	[more]
AT4G04460.1	6.2e-203	65.95	Saposin-like aspartyl protease family protein	[more]
AT4G04460.2	4.1e-199	65.36	Saposin-like aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 242..255 score: 45.81 coord: 292..303 score: 58.07 coord: 96..116 score: 73.01 coord: 487..502 score: 58.88
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47966	BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED	coord: 13..514
IPR033121	Peptidase family A1 domain	PFAM	PF00026	Asp	coord: 89..513 e-value: 4.7E-131 score: 437.5
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 90..511 score: 73.45771
IPR007856	Saposin-like type B, region 1	PFAM	PF05184	SapB_1	coord: 387..423 e-value: 4.7E-13 score: 48.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 31..248 e-value: 1.3E-79 score: 268.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 249..514 e-value: 1.6E-122 score: 409.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 77..513
None	No IPR available	GENE3D	1.10.225.10		coord: 320..423 e-value: 1.6E-122 score: 409.4
None	No IPR available	PANTHER	PTHR47966:SF31	ASPARTIC PROTEINASE-LIKE	coord: 13..514
IPR008138	Saposin B type, region 2	PFAM	PF03489	SapB_2	coord: 323..356 e-value: 2.2E-12 score: 47.0
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 105..116
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 292..303
IPR008139	Saposin B type domain	PROSITE	PS50015	SAP_B	coord: 384..425 score: 12.1696
IPR008139	Saposin B type domain	PROSITE	PS50015	SAP_B	coord: 320..360 score: 13.245999
IPR033869	Phytepsin	CDD	cd06098	phytepsin	coord: 80..512 e-value: 0.0 score: 608.988
IPR011001	Saposin-like	SUPERFAMILY	47862	Saposin	coord: 321..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07556-RA	Carg07556-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005773	vacuole
molecular_function	GO:0004190	aspartic-type endopeptidase activity