Carg07544 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07544
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSPARK domain-containing protein
LocationCarg_Chr13: 7733021 .. 7736653 (-)
RNA-Seq ExpressionCarg07544
SyntenyCarg07544
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACGCTGGCCCCCAAGGGGGGTGGTGGATTGTGAGATCCTACATCGGTTGGAGATGGAAACCCACATTCATTATAAGGGTGTGGAAACTTCTCTCTAGTAGGCGCGCTTTAAAACCTTGAGGGAATATCTAGAAGGGAACCTCATTAGTGAACTAGCTCCTCCGCCTGCTTGTTCGTCTCTCAAATTAGAATATTTATGTCACCTAAAACTGCAGAACATCATTATACAATAAACTAAAAAATGGTTGTACTTCATATGTTTAACGCGGTATTATATCTTACAAGTTATTTTTTTTCTTTTTGTTGCAGATTTCCATGAAACTAGCTGCATCCCGTCAACATATCCAACACGGCATCTGTCGAATGAGGAACCAATGGATGATATGTATCCTGAGATCGCTCCAAGTGGGAATCCAAAGCCATTCCTTCCTCTCCTTGCCCCTTCTCCATTAGCACCCTTCACAAATACCACAGTCCCAAACTTATCAGGTTTATCATTGTTTGTCTAGAAAGAGTTAGTTTATAATGATTCATTGATTCTTCATTTCAATTTAATCTCTCATACTCCTTACAGGACAATGTTTGTTGAACTTTTCTGCAACCGAAACTTTGATGGGTGTGACTGCCATGGATTGTTGGGCCCCTTTCGCGAAACAGATGGCCAATGTTATTTGTTGTCCACAGTTAGAAGCTACTCTTGCTATCCTTATTGGTCAGTCGAGTAAAGATACTAATGTTCTTGCTATAAACGGGACGCTTGCCGAATATTGCCTCTCAGATATTGAGCAGATTTTGGTGGGGCAGGGTGCAAATGAACGTCTTAAACACATATGCAGAGTTCATCCAGCCAATCTCACTGAAGGGTCTTGTCCGGCCAAAGATGTTAGTGAATTTGAGACCACTGTCGATACGTCTAAGTTGCTTGCTGCCTGTAATAAAATTGATCCTGTGAAAGAATGCTGCAATGCAATCTGCCAGAATGCCATTTCAGAAGCCGCGACAAAGTTAGCAATGATATCTACAGATTTCTTGGGTATGCCTGGATCTCAAGTCTTGCCCGAACAGTCCGCCAGGGTTCGTGATTGTAAAACTATTGTCCTCCGATGGCTGGCGAGCAAACTTCACCCTGCTAATGCCAAGGAAGTTCTTAGAGTACTGTCCAATTGCAATGTTAACAAAGGTGAGCTTGAAAGCAAATCTTTTCGAACGGGGATTTACGAAATTCTCGTGATATCACTGTTATAATTCATCCACATCTCTATGTATTTGTCGATCCTATGCCCATTTGCCTTGTTGGTCAATGTCTACAACATTTTCAATTTTTTAAGATTCGAGTCACCACTCACTTCTGATGCTATTATCTTTAGCTACCACATCATCATAGATCTTTTCCGTGTGCTATTCTCAGTGTGTCCATTGGAATTCCCCGACATGAAATATGTTGCCGATGCTTGCGGGAATGCGATCTCTAACAAGACAGGGTGCTGCCTGGCCATGGAAAGCTATGTAACGCATCTGCAAAAGCAGAGCCTCGTTACCAACCTACAAGCTTTGGATTGTGCTACATCATTGGAAATGAAACTACGAAAGTCGAATATCACGAAGAACGTGTACGACCTATGCCATATAAGCCTTAAGGATTTCTCCCTCCAAGGTTTGTAGCTTTTATCCTCTTATGTTTCCAGATTTAAACAACTCCTTGAATAATTTCACATGGATTCTAATAGTTTTTTTTTCCCTTTCCTGCCCATGTTCTAACTTGATAATGGATCTACAACTTTGAGCATTTCAGTTGGAAATCAAGGTACTATTTCATAAAAATTTACTTATTCCATATTCTTGCAAGCTAAACCTTTGATTACACCATTAGCTTTTGTTGGCTTAAAAGTTATTAGAAGACATACTGTTGGGATGTTCTTAGTTTTTAACGAAAAAAGAAAGAACGAAGAAGATTGGTGTCGGTCCATAGACACGAATGCAGATTTTTAGCCTGTTTTCAAAATTCAAATGATTCATTTTCTCTCTAACTTTCATTATCGTCTCGACAGAGTTTGGATGCCTTCTACCAAGCCTGCCTTCAGATGCCATATTTGATCCATCATCAGGGATCAGCTTTGTTTGTGATTTGAATGACCATATTCCAGCTCCATGGTCTTCTTCAACTCAAATGACAGCTTCATCATGCAATAAGAGTAGGACACATTTTTTTAAAACTTCCAGTATTTGGAGCATGGGTATCAAAGATAGACCCTTAACCAGTGTTCCCATCTTTATAAGAAAATGTCGAGTTATCGTCTTCTTAAATTTCTAATTGCTCGCGTTTTTGTATTGCAGCTATCAAAATTCCTGCACTTCCCGCAGCAGCATCTGCTCAAAGTGGTACTTATATTCTTCAACTATCCTCGAATTTCTCTAAAATACGAATATCATTACTAGTGGTCATTTTGAAACAGTAGTTTAATTTCATCATCATTCTTCTCCATGAGATCCCACATCGGTTGGAGAGGTGAACGAAACATTCATTATAAGGGTGTGGAAACCTCTCCCTAGTAGACACGTTTTAAAACCGTGAGGCTAATGGCGATACGTAACAAGCCAAAACGGACAATATCTGCTAGCGGTGGATTTGGCTGTTACAAATGGTATTAGAGCTAGGCATCGAGCGGTGTGCCAGCGAGTACGCCAAACCCCTAAAGGGGTGGATTGTGAGATCCCACATCCGTTGGAGAGGAGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTTTCCCTAGTAGATGTGTTTTAAAACTGCGAGGCTGACGACGATATGTAACTAGCCAAAGCGGATAATACCTGCTAGCGGTGGGCTTGGGTTGTTACACTATCAATCTAATCCCATCCACCAAACACAATTTATACGAACAAGTGGATTCTTTGATCCATTCTTTTACACAAACAGTGGTTACAAAAATGTAGAATTTTCAATCTCGAACTTAGTCAACGATTACAAAACAAGTCATTACTAACTAATGCTCACTGGGATGAATACAGAATTTAATACCATGCTTTAATTGACCTGACATCTGAGTTCTGAGGTAATAGAAGAAAAAAAAGACTTAAGTGAGTCAGTGAGAGTTATGTAGATACAGTATCAAAGTCAAAAAATGCCGAGTTTTGTTAGGTTGGTTGACACATTCAAAGTGGATTTCCTCAAACTTAATACCCCAACTGTTGCAGGCCTTTACAACGATGGTGTGGTACATGTGTTGCTCATTGCTATCTCAGTGGCCCTCATGATACTCATCTAGCTTATTTATTGCTATCTTTGGAGAAGACTGTTGTCAAGTCATCATCTAAAACAAGGATGTTCATGTTCATGTTCATGTTCAATGAAGCTTATGACGAAGTCTTGTATCCTCAGAAGTTTGATGATCCTTGTGAATAGTTTTAGATGTTCTGAATGTGGTAGAAATTTGAGTTGGAAAAATGGTTCCCAAATTCCTAATTTGTTCATTCTTGCTGTCAATGTAATAAAAAAGTTTTGTAAAGATATATTAGGAAAGCTGAGCAAAGTGGTGCAACATTTCAGATTTCGTTGTATATTTGGATTTCTATCCAACATGAACATAGCTCAATAGTGATTGACGTGT

mRNA sequence

CGACGCTGGCCCCCAAGGGGGGTGGTGGATTGTGAGATCCTACATCGGTTGGAGATGGAAACCCACATTCATTATAAGGGTGTGGAAACTTCTCTCTAGTAGGCGCGCTTTAAAACCTTGAGGGAATATCTAGAAGGGAACCTCATTAGTGAACTAGCTCCTCCGCCTGCTTGTTCGTCTCTCAAATTAGAATATTTATGTCACCTAAAACTGCAGAACATCATTATACAATAAACTAAAAAATGGTTGTACTTCATATGTTTAACGCGGTATTATATCTTACAAGTTATTTTTTTTCTTTTTGTTGCAGATTTCCATGAAACTAGCTGCATCCCGTCAACATATCCAACACGGCATCTGTCGAATGAGGAACCAATGGATGATATGTATCCTGAGATCGCTCCAAGTGGGAATCCAAAGCCATTCCTTCCTCTCCTTGCCCCTTCTCCATTAGCACCCTTCACAAATACCACAGTCCCAAACTTATCAGGACAATGTTTGTTGAACTTTTCTGCAACCGAAACTTTGATGGGTGTGACTGCCATGGATTGTTGGGCCCCTTTCGCGAAACAGATGGCCAATGTTATTTGTTGTCCACAGTTAGAAGCTACTCTTGCTATCCTTATTGGTCAGTCGAGTAAAGATACTAATGTTCTTGCTATAAACGGGACGCTTGCCGAATATTGCCTCTCAGATATTGAGCAGATTTTGGTGGGGCAGGGTGCAAATGAACGTCTTAAACACATATGCAGAGTTCATCCAGCCAATCTCACTGAAGGGTCTTGTCCGGCCAAAGATGTTAGTGAATTTGAGACCACTGTCGATACGTCTAAGTTGCTTGCTGCCTGTAATAAAATTGATCCTGTGAAAGAATGCTGCAATGCAATCTGCCAGAATGCCATTTCAGAAGCCGCGACAAAGTTAGCAATGATATCTACAGATTTCTTGGGTATGCCTGGATCTCAAGTCTTGCCCGAACAGTCCGCCAGGGTTCGTGATTGTAAAACTATTGTCCTCCGATGGCTGGCGAGCAAACTTCACCCTGCTAATGCCAAGGAAGTTCTTAGAGTACTGTCCAATTGCAATGTTAACAAAGTGTGTCCATTGGAATTCCCCGACATGAAATATGTTGCCGATGCTTGCGGGAATGCGATCTCTAACAAGACAGGGTGCTGCCTGGCCATGGAAAGCTATGTAACGCATCTGCAAAAGCAGAGCCTCGTTACCAACCTACAAGCTTTGGATTGTGCTACATCATTGGAAATGAAACTACGAAAGTCGAATATCACGAAGAACGTGTACGACCTATGCCATATAAGCCTTCAAATGATTCATTTTCTCTCTAACTTTCATTATCGTCTCGACAGAGTTTGGATGCCTTCTACCAAGCCTGCCTTCAGATGCCATATTTGATCCATCATCAGGGATCAGCTTTGTTTGTGATTTGAATGACCATATTCCAGCTCCATGGTCTTCTTCAACTCAAATGACAGCTTCATCATGCAATAAGACTATCAAAATTCCTGCACTTCCCGCAGCAGCATCTGCTCAAAGTGGCCTTTACAACGATGGTGTGGTACATGTGTTGCTCATTGCTATCTCAGTGGCCCTCATGATACTCATCTAGCTTATTTATTGCTATCTTTGGAGAAGACTGTTGTCAAGTCATCATCTAAAACAAGGATGTTCATGTTCATGTTCATGTTCAATGAAGCTTATGACGAAGTCTTGTATCCTCAGAAGTTTGATGATCCTTGTGAATAGTTTTAGATGTTCTGAATGTGGTAGAAATTTGAGTTGGAAAAATGGTTCCCAAATTCCTAATTTGTTCATTCTTGCTGTCAATGTAATAAAAAAGTTTTGTAAAGATATATTAGGAAAGCTGAGCAAAGTGGTGCAACATTTCAGATTTCGTTGTATATTTGGATTTCTATCCAACATGAACATAGCTCAATAGTGATTGACGTGT

Coding sequence (CDS)

ATGGATGATATGTATCCTGAGATCGCTCCAAGTGGGAATCCAAAGCCATTCCTTCCTCTCCTTGCCCCTTCTCCATTAGCACCCTTCACAAATACCACAGTCCCAAACTTATCAGGACAATGTTTGTTGAACTTTTCTGCAACCGAAACTTTGATGGGTGTGACTGCCATGGATTGTTGGGCCCCTTTCGCGAAACAGATGGCCAATGTTATTTGTTGTCCACAGTTAGAAGCTACTCTTGCTATCCTTATTGGTCAGTCGAGTAAAGATACTAATGTTCTTGCTATAAACGGGACGCTTGCCGAATATTGCCTCTCAGATATTGAGCAGATTTTGGTGGGGCAGGGTGCAAATGAACGTCTTAAACACATATGCAGAGTTCATCCAGCCAATCTCACTGAAGGGTCTTGTCCGGCCAAAGATGTTAGTGAATTTGAGACCACTGTCGATACGTCTAAGTTGCTTGCTGCCTGTAATAAAATTGATCCTGTGAAAGAATGCTGCAATGCAATCTGCCAGAATGCCATTTCAGAAGCCGCGACAAAGTTAGCAATGATATCTACAGATTTCTTGGGTATGCCTGGATCTCAAGTCTTGCCCGAACAGTCCGCCAGGGTTCGTGATTGTAAAACTATTGTCCTCCGATGGCTGGCGAGCAAACTTCACCCTGCTAATGCCAAGGAAGTTCTTAGAGTACTGTCCAATTGCAATGTTAACAAAGTGTGTCCATTGGAATTCCCCGACATGAAATATGTTGCCGATGCTTGCGGGAATGCGATCTCTAACAAGACAGGGTGCTGCCTGGCCATGGAAAGCTATGTAACGCATCTGCAAAAGCAGAGCCTCGTTACCAACCTACAAGCTTTGGATTGTGCTACATCATTGGAAATGAAACTACGAAAGTCGAATATCACGAAGAACGTGTACGACCTATGCCATATAAGCCTTCAAATGATTCATTTTCTCTCTAACTTTCATTATCGTCTCGACAGAGTTTGGATGCCTTCTACCAAGCCTGCCTTCAGATGCCATATTTGA

Protein sequence

MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWAPFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERLKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAATKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKVCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRKSNITKNVYDLCHISLQMIHFLSNFHYRLDRVWMPSTKPAFRCHI
Homology
BLAST of Carg07544 vs. NCBI nr
Match: KAG7019686.1 (putative GPI-anchored protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER
Sbjct: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300

Query: 301 KSNITKNVYDLCHISLQMIHFLSNFHYRLDRVWMPSTKPAFRCHI 346
           KSNITKNVYDLCHISLQMIHFLSNFHYRLDRVWMPSTKPAFRCHI
Sbjct: 301 KSNITKNVYDLCHISLQMIHFLSNFHYRLDRVWMPSTKPAFRCHI 345

BLAST of Carg07544 vs. NCBI nr
Match: KAG6584085.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 640.6 bits (1651), Expect = 7.9e-180
Identity = 316/317 (99.68%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 46  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 105

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER
Sbjct: 106 APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 165

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 166 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 225

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 226 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 285

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 286 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 345

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 346 KSNITKNVYDLCHISLK 362

BLAST of Carg07544 vs. NCBI nr
Match: XP_023519737.1 (uncharacterized GPI-anchored protein At1g61900 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.8 bits (1649), Expect = 1.3e-179
Identity = 315/317 (99.37%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 80  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 139

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDTNVLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 140 APFAKQMANVICCPQLEATLAILIGQSSKDTNVLALNGTLAEYCLSDIEQILVGQGANER 199

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 200 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 259

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 260 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 319

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 320 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 379

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 380 KSNITKNVYDLCHISLK 396

BLAST of Carg07544 vs. NCBI nr
Match: XP_022933208.1 (uncharacterized GPI-anchored protein At1g61900 [Cucurbita moschata])

HSP 1 Score: 639.8 bits (1649), Expect = 1.3e-179
Identity = 315/317 (99.37%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 46  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 105

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDTNVLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 106 APFAKQMANVICCPQLEATLAILIGQSSKDTNVLALNGTLAEYCLSDIEQILVGQGANER 165

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 166 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 225

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 226 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 285

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 286 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 345

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 346 KSNITKNVYDLCHISLK 362

BLAST of Carg07544 vs. NCBI nr
Match: XP_023001253.1 (uncharacterized GPI-anchored protein At1g61900 isoform X1 [Cucurbita maxima])

HSP 1 Score: 636.7 bits (1641), Expect = 1.1e-178
Identity = 314/317 (99.05%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 80  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 139

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDT VLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 140 APFAKQMANVICCPQLEATLAILIGQSSKDTYVLALNGTLAEYCLSDIEQILVGQGANER 199

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 200 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 259

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 260 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 319

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 320 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 379

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 380 KSNITKNVYDLCHISLK 396

BLAST of Carg07544 vs. ExPASy Swiss-Prot
Match: Q8GUI4 (Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana OX=3702 GN=At1g61900 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.9e-108
Identity = 181/316 (57.28%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 2   DDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWA 61
           ++  PEI+P  +P+PFLP +APSP+ P+ N+T+P LSG C LNFSA+E+L+  T+ +CW 
Sbjct: 30  EEFLPEISPDTSPQPFLPFIAPSPMVPYINSTMPKLSGLCSLNFSASESLIQTTSHNCWT 89

Query: 62  PFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERL 121
            FA  +ANV+CCPQL+ATL I++G++SK+T +LA+N T +++CLSD+EQILVG+GA+ +L
Sbjct: 90  VFAPLLANVMCCPQLDATLTIILGKASKETGLLALNRTQSKHCLSDLEQILVGKGASGQL 149

Query: 122 KHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAAT 181
             IC +H +NLT  SCP  +V EFE+TVDT+KLL AC KIDPVKECC   CQNAI +AAT
Sbjct: 150 NKICSIHSSNLTSSSCPVINVDEFESTVDTAKLLLACEKIDPVKECCEEACQNAILDAAT 209

Query: 182 KLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKV 241
            ++        +  S+ L + S R+ DCK +V RWLA+KL P+  KE LR L+NC +N+V
Sbjct: 210 NIS--------LKASETLTDNSDRINDCKNVVNRWLATKLDPSRVKETLRGLANCKINRV 269

Query: 242 CPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRK 301
           CPL FP MK++   C N +SN+TGCC AMESYV+HLQKQ+L+TNLQALDCATSL  KL+K
Sbjct: 270 CPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCATSLGTKLQK 329

Query: 302 SNITKNVYDLCHISLQ 318
            NITKN++ +CHISL+
Sbjct: 330 LNITKNIFSVCHISLK 337

BLAST of Carg07544 vs. ExPASy TrEMBL
Match: A0A6J1F438 (uncharacterized GPI-anchored protein At1g61900 OS=Cucurbita moschata OX=3662 GN=LOC111440054 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.5e-180
Identity = 315/317 (99.37%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 46  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 105

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDTNVLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 106 APFAKQMANVICCPQLEATLAILIGQSSKDTNVLALNGTLAEYCLSDIEQILVGQGANER 165

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 166 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 225

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 226 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 285

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 286 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 345

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 346 KSNITKNVYDLCHISLK 362

BLAST of Carg07544 vs. ExPASy TrEMBL
Match: A0A6J1KM81 (uncharacterized GPI-anchored protein At1g61900 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495441 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.5e-179
Identity = 314/317 (99.05%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 80  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 139

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDT VLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 140 APFAKQMANVICCPQLEATLAILIGQSSKDTYVLALNGTLAEYCLSDIEQILVGQGANER 199

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 200 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 259

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 260 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 319

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 320 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 379

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 380 KSNITKNVYDLCHISLK 396

BLAST of Carg07544 vs. ExPASy TrEMBL
Match: A0A6J1KPZ7 (uncharacterized GPI-anchored protein At1g61900 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495441 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.5e-179
Identity = 314/317 (99.05%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW
Sbjct: 80  MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 139

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSSKDT VLA+NGTLAEYCLSDIEQILVGQGANER
Sbjct: 140 APFAKQMANVICCPQLEATLAILIGQSSKDTYVLALNGTLAEYCLSDIEQILVGQGANER 199

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 200 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 259

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 260 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 319

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR
Sbjct: 320 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 379

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITKNVYDLCHISL+
Sbjct: 380 KSNITKNVYDLCHISLK 396

BLAST of Carg07544 vs. ExPASy TrEMBL
Match: A0A5A7UP39 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G00290 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.5e-171
Identity = 304/350 (86.86%), Postives = 321/350 (91.71%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLP LAPSPL PFTNTTVP LSGQCLLNFSATETLM +TA+DCW
Sbjct: 1   MDDMYPEIAPSGNPKPFLPFLAPSPLVPFTNTTVPKLSGQCLLNFSATETLMSMTAIDCW 60

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSS+DTNVLA+NGTLA+YCLSDIEQILVGQGA+ER
Sbjct: 61  APFAKQMANVICCPQLEATLAILIGQSSQDTNVLALNGTLAKYCLSDIEQILVGQGASER 120

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           L+HIC VHPANLTEGSCPAKD+SEFE TVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 121 LRHICTVHPANLTEGSCPAKDISEFEATVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TK+AMISTDFLGMPGSQVLPEQS RVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 181 TKIAMISTDFLGMPGSQVLPEQSTRVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKT CCLAMESYVTHLQKQSLVTNLQALDCAT+LEMKLR
Sbjct: 241 VCPLEFPDMKYVADACGNAISNKTACCLAMESYVTHLQKQSLVTNLQALDCATALEMKLR 300

Query: 301 KSNITKNVYDLCHISLQMIHFLSNFHYRL-----DRVWMPSTKPAFRCHI 346
           KSNITK+VY LCHISL+    L  F   L     D ++ PS+  +F C +
Sbjct: 301 KSNITKDVYGLCHISLKDFS-LQEFGCLLPSLPSDAIFDPSSGISFVCDL 349

BLAST of Carg07544 vs. ExPASy TrEMBL
Match: A0A5D3BJB0 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005810 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 296/317 (93.38%), Postives = 308/317 (97.16%), Query Frame = 0

Query: 1   MDDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCW 60
           MDDMYPEIAPSGNPKPFLP LAPSPL PFTNTTVP LSGQCLLNFSATETLM +TA+DCW
Sbjct: 1   MDDMYPEIAPSGNPKPFLPFLAPSPLVPFTNTTVPKLSGQCLLNFSATETLMSMTAIDCW 60

Query: 61  APFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANER 120
           APFAKQMANVICCPQLEATLAILIGQSS+DTNVLA+NGTLA+YCLSDIEQILVGQGA+ER
Sbjct: 61  APFAKQMANVICCPQLEATLAILIGQSSQDTNVLALNGTLAKYCLSDIEQILVGQGASER 120

Query: 121 LKHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180
           L+HIC VHPANLTEGSCPAKD+SEFE TVDTSKLLAACNKIDPVKECCNAICQNAISEAA
Sbjct: 121 LRHICTVHPANLTEGSCPAKDISEFEATVDTSKLLAACNKIDPVKECCNAICQNAISEAA 180

Query: 181 TKLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240
           TK+AMISTDFLGMPGSQVLPEQS RVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK
Sbjct: 181 TKIAMISTDFLGMPGSQVLPEQSTRVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNK 240

Query: 241 VCPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLR 300
           VCPLEFPDMKYVADACGNAISNKT CCLAMESYVTHLQKQSLVTNLQALDCAT+LEMKLR
Sbjct: 241 VCPLEFPDMKYVADACGNAISNKTACCLAMESYVTHLQKQSLVTNLQALDCATALEMKLR 300

Query: 301 KSNITKNVYDLCHISLQ 318
           KSNITK+VY LCHISL+
Sbjct: 301 KSNITKDVYGLCHISLK 317

BLAST of Carg07544 vs. TAIR 10
Match: AT1G61900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1); Has 65 Blast hits to 65 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 65; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 392.5 bits (1007), Expect = 3.5e-109
Identity = 181/316 (57.28%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 2   DDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWA 61
           ++  PEI+P  +P+PFLP +APSP+ P+ N+T+P LSG C LNFSA+E+L+  T+ +CW 
Sbjct: 30  EEFLPEISPDTSPQPFLPFIAPSPMVPYINSTMPKLSGLCSLNFSASESLIQTTSHNCWT 89

Query: 62  PFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERL 121
            FA  +ANV+CCPQL+ATL I++G++SK+T +LA+N T +++CLSD+EQILVG+GA+ +L
Sbjct: 90  VFAPLLANVMCCPQLDATLTIILGKASKETGLLALNRTQSKHCLSDLEQILVGKGASGQL 149

Query: 122 KHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAAT 181
             IC +H +NLT  SCP  +V EFE+TVDT+KLL AC KIDPVKECC   CQNAI +AAT
Sbjct: 150 NKICSIHSSNLTSSSCPVINVDEFESTVDTAKLLLACEKIDPVKECCEEACQNAILDAAT 209

Query: 182 KLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKV 241
            ++        +  S+ L + S R+ DCK +V RWLA+KL P+  KE LR L+NC +N+V
Sbjct: 210 NIS--------LKASETLTDNSDRINDCKNVVNRWLATKLDPSRVKETLRGLANCKINRV 269

Query: 242 CPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRK 301
           CPL FP MK++   C N +SN+TGCC AMESYV+HLQKQ+L+TNLQALDCATSL  KL+K
Sbjct: 270 CPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCATSLGTKLQK 329

Query: 302 SNITKNVYDLCHISLQ 318
            NITKN++ +CHISL+
Sbjct: 330 LNITKNIFSVCHISLK 337

BLAST of Carg07544 vs. TAIR 10
Match: AT1G61900.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 392.5 bits (1007), Expect = 3.5e-109
Identity = 181/316 (57.28%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 2   DDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWA 61
           ++  PEI+P  +P+PFLP +APSP+ P+ N+T+P LSG C LNFSA+E+L+  T+ +CW 
Sbjct: 30  EEFLPEISPDTSPQPFLPFIAPSPMVPYINSTMPKLSGLCSLNFSASESLIQTTSHNCWT 89

Query: 62  PFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERL 121
            FA  +ANV+CCPQL+ATL I++G++SK+T +LA+N T +++CLSD+EQILVG+GA+ +L
Sbjct: 90  VFAPLLANVMCCPQLDATLTIILGKASKETGLLALNRTQSKHCLSDLEQILVGKGASGQL 149

Query: 122 KHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAAT 181
             IC +H +NLT  SCP  +V EFE+TVDT+KLL AC KIDPVKECC   CQNAI +AAT
Sbjct: 150 NKICSIHSSNLTSSSCPVINVDEFESTVDTAKLLLACEKIDPVKECCEEACQNAILDAAT 209

Query: 182 KLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKV 241
            ++        +  S+ L + S R+ DCK +V RWLA+KL P+  KE LR L+NC +N+V
Sbjct: 210 NIS--------LKASETLTDNSDRINDCKNVVNRWLATKLDPSRVKETLRGLANCKINRV 269

Query: 242 CPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRK 301
           CPL FP MK++   C N +SN+TGCC AMESYV+HLQKQ+L+TNLQALDCATSL  KL+K
Sbjct: 270 CPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCATSLGTKLQK 329

Query: 302 SNITKNVYDLCHISLQ 318
            NITKN++ +CHISL+
Sbjct: 330 LNITKNIFSVCHISLK 337

BLAST of Carg07544 vs. TAIR 10
Match: AT1G61900.3 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1). )

HSP 1 Score: 392.5 bits (1007), Expect = 3.5e-109
Identity = 181/316 (57.28%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 2   DDMYPEIAPSGNPKPFLPLLAPSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWA 61
           ++  PEI+P  +P+PFLP +APSP+ P+ N+T+P LSG C LNFSA+E+L+  T+ +CW 
Sbjct: 30  EEFLPEISPDTSPQPFLPFIAPSPMVPYINSTMPKLSGLCSLNFSASESLIQTTSHNCWT 89

Query: 62  PFAKQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERL 121
            FA  +ANV+CCPQL+ATL I++G++SK+T +LA+N T +++CLSD+EQILVG+GA+ +L
Sbjct: 90  VFAPLLANVMCCPQLDATLTIILGKASKETGLLALNRTQSKHCLSDLEQILVGKGASGQL 149

Query: 122 KHICRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAAT 181
             IC +H +NLT  SCP  +V EFE+TVDT+KLL AC KIDPVKECC   CQNAI +AAT
Sbjct: 150 NKICSIHSSNLTSSSCPVINVDEFESTVDTAKLLLACEKIDPVKECCEEACQNAILDAAT 209

Query: 182 KLAMISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKV 241
            ++        +  S+ L + S R+ DCK +V RWLA+KL P+  KE LR L+NC +N+V
Sbjct: 210 NIS--------LKASETLTDNSDRINDCKNVVNRWLATKLDPSRVKETLRGLANCKINRV 269

Query: 242 CPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRK 301
           CPL FP MK++   C N +SN+TGCC AMESYV+HLQKQ+L+TNLQALDCATSL  KL+K
Sbjct: 270 CPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCATSLGTKLQK 329

Query: 302 SNITKNVYDLCHISLQ 318
            NITKN++ +CHISL+
Sbjct: 330 LNITKNIFSVCHISLK 337

BLAST of Carg07544 vs. TAIR 10
Match: AT2G30700.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G61900.1); Has 68 Blast hits to 67 proteins in 13 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 66; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 235.0 bits (598), Expect = 9.3e-62
Identity = 127/316 (40.19%), Postives = 178/316 (56.33%), Query Frame = 0

Query: 7   EIAPSGNPKPFLPLL--APSPLAPFTNTTVPNLSGQCLLNFSATETLMGVTAMDCWAPFA 66
           +++PS  PK   P L   P     F +T  P L+G+C  +F A  +++   A DC  PFA
Sbjct: 69  QVSPSVIPKYASPALPWTPPMYPTFPDTYEPKLTGKCPTDFQAISSVIDTAASDCSQPFA 128

Query: 67  KQMANVICCPQLEATLAILIGQSSKDTNVLAINGTLAEYCLSDIEQILVGQGANERLKHI 126
             + NVICCPQ  + L I  GQ +  +N L +   +A  C SDI  ILV + AN  +  +
Sbjct: 129 ALVGNVICCPQFVSLLHIFQGQHNVKSNKLVLPDAVATDCFSDIVSILVSRRANMTIPAL 188

Query: 127 CRVHPANLTEGSCPAKDVSEFETTVDTSKLLAACNKIDPVKECCNAICQNAISEAATKLA 186
           C V  +NLT GSCP  DV+ FE  V++SKLL AC  +DP+KECC  ICQ AI EAA  ++
Sbjct: 189 CSVTSSNLTGGSCPVTDVTTFEKVVNSSKLLDACRTVDPLKECCRPICQPAIMEAALIIS 248

Query: 187 ---MISTDFLGMPGSQVLPEQSARVRDCKTIVLRWLASKLHPANAKEVLRVLSNCNVNKV 246
              M   D + + GS         + DCK +V  +L+ KL    A    R+LS+C VNK 
Sbjct: 249 GHQMTVGDKIPLAGS----NNVNAINDCKNVVFSYLSRKLPADKANAAFRILSSCKVNKA 308

Query: 247 CPLEFPDMKYVADACGNAISNKTGCCLAMESYVTHLQKQSLVTNLQALDCATSLEMKLRK 306
           CPLEF +   V  AC N  +    CC ++ +Y++ +Q Q L+TN QA+ CAT +   LRK
Sbjct: 309 CPLEFKEPTEVIKACRNVAAPSPSCCSSLNAYISGIQNQMLITNKQAIVCATVIGSMLRK 368

Query: 307 SNITKNVYDLCHISLQ 318
             +  N+Y+LC + L+
Sbjct: 369 GGVMTNIYELCDVDLK 380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019686.12.6e-199100.00putative GPI-anchored protein [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6584085.17.9e-18099.68putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023519737.11.3e-17999.37uncharacterized GPI-anchored protein At1g61900 [Cucurbita pepo subsp. pepo][more]
XP_022933208.11.3e-17999.37uncharacterized GPI-anchored protein At1g61900 [Cucurbita moschata][more]
XP_023001253.11.1e-17899.05uncharacterized GPI-anchored protein At1g61900 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8GUI44.9e-10857.28Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1F4386.5e-18099.37uncharacterized GPI-anchored protein At1g61900 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KM815.5e-17999.05uncharacterized GPI-anchored protein At1g61900 isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1KPZ75.5e-17999.05uncharacterized GPI-anchored protein At1g61900 isoform X1 OS=Cucurbita maxima OX... [more]
A0A5A7UP395.5e-17186.86Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3BJB02.1e-17093.38Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT1G61900.13.5e-10957.28unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G61900.23.5e-10957.28unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G61900.33.5e-10957.28unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G30700.19.3e-6240.19unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043891SPARK domainPFAMPF19160SPARKcoord: 37..183
e-value: 3.0E-22
score: 79.7
IPR040336Uncharacterized GPI-anchored protein At1g61900-lkePANTHERPTHR33831GPI-ANCHORED PROTEINcoord: 2..317
NoneNo IPR availablePANTHERPTHR33831:SF5OS07G0102300 PROTEINcoord: 2..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07544-RACarg07544-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane