Carg07225 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg07225 vs. NCBI nr
Match: KAG7014416.1 (Piezo-type mechanosensitive ion channel-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 4770.7 bits (12373), Expect = 0.0e+00
Identity = 2407/2407 (100.00%), Postives = 2407/2407 (100.00%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIVTFLVIWSLEGYQWSIA 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIVTFLVIWSLEGYQWSIA
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIVTFLVIWSLEGYQWSIA 60

Query: 61   NAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVAAADIFWYRIGFVQRRDSCWVHLFSF 120
            NAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVAAADIFWYRIGFVQRRDSCWVHLFSF
Sbjct: 61   NAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVAAADIFWYRIGFVQRRDSCWVHLFSF 120

Query: 121  VDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWR 180
            VDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWR
Sbjct: 121  VDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPFFIGSCVGLVDWSLTSNFLGLFRWWR 180

Query: 181  PLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGLFKVSSTPEWPGICSYISLILFYIML 240
            PLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGLFKVSSTPEWPGICSYISLILFYIML
Sbjct: 181  PLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGLFKVSSTPEWPGICSYISLILFYIML 240

Query: 241  SCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINF 300
            SCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINF
Sbjct: 241  SCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIRELRSGVKHTNVLLRREVFRTFTINF 300

Query: 301  FTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIVYAFPSLFQLHRLNGLLLVFILFWAI 360
            FTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIVYAFPSLFQLHRLNGLLLVFILFWAI
Sbjct: 301  FTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIVYAFPSLFQLHRLNGLLLVFILFWAI 360

Query: 361  STYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFCLGILVALVNLVNNSVFL 420
            STYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFCLGILVALVNLVNNSVFL
Sbjct: 361  STYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPGFFLLAQFCLGILVALVNLVNNSVFL 420

Query: 421  CVSDEDERSSNDNCSAREAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVY 480
            CVSDEDERSSNDNCSAREAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVY
Sbjct: 421  CVSDEDERSSNDNCSAREAGETKVLIVATIAWGLRKSSRAIVLTLIFLVAMKPGFIHAVY 480

Query: 481  VVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLLQINLISNVLDREGSLCREILLQLGL 540
            VVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLLQINLISNVLDREGSLCREILLQLGL
Sbjct: 481  VVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLLQINLISNVLDREGSLCREILLQLGL 540

Query: 541  LGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAIVQHTPSPPVGFSILRAGLNKSVLLS 600
            LGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAIVQHTPSPPVGFSILRAGLNKSVLLS
Sbjct: 541  LGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAIVQHTPSPPVGFSILRAGLNKSVLLS 600

Query: 601  VYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYIS 660
            VYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYIS
Sbjct: 601  VYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMYRSCGTYIAFLTILLTVFFVKPNYIS 660

Query: 661  FGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIVFIFIYCLSSFPSFRIWLSRSIDLYF 720
            FGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIVFIFIYCLSSFPSFRIWLSRSIDLYF
Sbjct: 661  FGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIVFIFIYCLSSFPSFRIWLSRSIDLYF 720

Query: 721  FLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSRYNSSDEPEPLEYGRLGFVKRFLSWH 780
            FLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSRYNSSDEPEPLEYGRLGFVKRFLSWH
Sbjct: 721  FLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSRYNSSDEPEPLEYGRLGFVKRFLSWH 780

Query: 781  SDKILFASLFYASISPISAFGLLYLLGLGVCSTLPKISHIPSKLFLAYTGVIMTAEYLFQ 840
            SDKILFASLFYASISPISAFGLLYLLGLGVCSTLPKISHIPSKLFLAYTGVIMTAEYLFQ
Sbjct: 781  SDKILFASLFYASISPISAFGLLYLLGLGVCSTLPKISHIPSKLFLAYTGVIMTAEYLFQ 840

Query: 841  MWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLELGLRGKVLIIAACTLQYNVFRWLERV 900
            MWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLELGLRGKVLIIAACTLQYNVFRWLERV
Sbjct: 841  MWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLELGLRGKVLIIAACTLQYNVFRWLERV 900

Query: 901  PGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNPSLDAGRLFVQQEGSDHILRPSFVSS 960
            PGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNPSLDAGRLFVQQEGSDHILRPSFVSS
Sbjct: 901  PGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNPSLDAGRLFVQQEGSDHILRPSFVSS 960

Query: 961  QSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKECHKWDKMRIIYWRKERFEMQKIIFKI 1020
            QSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKECHKWDKMRIIYWRKERFEMQKIIFKI
Sbjct: 961  QSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKECHKWDKMRIIYWRKERFEMQKIIFKI 1020

Query: 1021 YMKFWMENLFHLFGLEITMISLLLASFAVLNSVSLFYVGLLAACILLDRGIIRKLWPIFV 1080
            YMKFWMENLFHLFGLEITMISLLLASFAVLNSVSLFYVGLLAACILLDRGIIRKLWPIFV
Sbjct: 1021 YMKFWMENLFHLFGLEITMISLLLASFAVLNSVSLFYVGLLAACILLDRGIIRKLWPIFV 1080

Query: 1081 FLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHDCWRISNLYFQFCLNCWLGLTVDDSR 1140
            FLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHDCWRISNLYFQFCLNCWLGLTVDDSR
Sbjct: 1081 FLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHDCWRISNLYFQFCLNCWLGLTVDDSR 1140

Query: 1141 MLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVSHRKNTFVWRDLSFETKSMWTILDYL 1200
            MLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVSHRKNTFVWRDLSFETKSMWTILDYL
Sbjct: 1141 MLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVSHRKNTFVWRDLSFETKSMWTILDYL 1200

Query: 1201 RLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYN 1260
            RLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYN
Sbjct: 1201 RLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFALVFFRLRLEILKKKNKVFKFLRAYN 1260

Query: 1261 FALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVS 1320
            FALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVS
Sbjct: 1261 FALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGFYKYDYGFRITARSALVEIIIFMLVS 1320

Query: 1321 LQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAAWKTEQLQHIRDSEESKRKRNLQVEK 1380
            LQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAAWKTEQLQHIRDSEESKRKRNLQVEK
Sbjct: 1321 LQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAAWKTEQLQHIRDSEESKRKRNLQVEK 1380

Query: 1381 MKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLRKQSTSRMDDDAMTSDIEGTLLGKVE 1440
            MKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLRKQSTSRMDDDAMTSDIEGTLLGKVE
Sbjct: 1381 MKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLRKQSTSRMDDDAMTSDIEGTLLGKVE 1440

Query: 1441 QMIRTDSSPFPELQESLANLRTESRMHSMEFSVPEICEIDSQISDISLDLDRKRKHRSSA 1500
            QMIRTDSSPFPELQESLANLRTESRMHSMEFSVPEICEIDSQISDISLDLDRKRKHRSSA
Sbjct: 1441 QMIRTDSSPFPELQESLANLRTESRMHSMEFSVPEICEIDSQISDISLDLDRKRKHRSSA 1500

Query: 1501 KGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVTQDDDMMEQGNTEDRVYDQIESQE 1560
            KGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVTQDDDMMEQGNTEDRVYDQIESQE
Sbjct: 1501 KGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLNVTQDDDMMEQGNTEDRVYDQIESQE 1560

Query: 1561 TRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLS 1620
            TRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLS
Sbjct: 1561 TRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLS 1620

Query: 1621 MVYLAALFVYALCVNTGPGYMFWVIMLIYTELYILLQYLYQIIIQHCGLTINSDLLQELG 1680
            MVYLAALFVYALCVNTGPGYMFWVIMLIYTELYILLQYLYQIIIQHCGLTINSDLLQELG
Sbjct: 1621 MVYLAALFVYALCVNTGPGYMFWVIMLIYTELYILLQYLYQIIIQHCGLTINSDLLQELG 1680

Query: 1681 FPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDGEWTYSNAFNKNDLPRKHSLGHYGLT 1740
            FPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDGEWTYSNAFNKNDLPRKHSLGHYGLT
Sbjct: 1681 FPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDGEWTYSNAFNKNDLPRKHSLGHYGLT 1740

Query: 1741 EKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIES 1800
            EKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIES
Sbjct: 1741 EKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIES 1800

Query: 1801 GINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCA 1860
            GINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCA
Sbjct: 1801 GINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCA 1860

Query: 1861 EWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQILSVIGGGKREIDLYAYVFGADLIVFF 1920
            EWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQILSVIGGGKREIDLYAYVFGADLIVFF
Sbjct: 1861 EWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQILSVIGGGKREIDLYAYVFGADLIVFF 1920

Query: 1921 FVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFY 1980
            FVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFY
Sbjct: 1921 FVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFY 1980

Query: 1981 LFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYR 2040
            LFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYR
Sbjct: 1981 LFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYR 2040

Query: 2041 QFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC 2100
            QFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC
Sbjct: 2041 QFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC 2100

Query: 2101 DAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQ 2160
            DAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQ
Sbjct: 2101 DAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQ 2160

Query: 2161 VDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPGGYLSPYNRDDIQLICCQADASVLWL 2220
            VDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPGGYLSPYNRDDIQLICCQADASVLWL
Sbjct: 2161 VDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPGGYLSPYNRDDIQLICCQADASVLWL 2220

Query: 2221 VPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLN 2280
            VPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLN
Sbjct: 2221 VPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLN 2280

Query: 2281 GSMNSFQIKNVYERYFRVTGSGDDNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGP 2340
            GSMNSFQIKNVYERYFRVTGSGDDNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGP
Sbjct: 2281 GSMNSFQIKNVYERYFRVTGSGDDNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGP 2340

Query: 2341 VAIVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDR 2400
            VAIVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDR
Sbjct: 2341 VAIVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYENLPSCDR 2400

Query: 2401 PDLILKP 2408
            PDLILKP
Sbjct: 2401 PDLILKP 2407

BLAST of Carg07225 vs. NCBI nr
Match: XP_022953508.1 (piezo-type mechanosensitive ion channel homolog [Cucurbita moschata])

HSP 1 Score: 4725.2 bits (12255), Expect = 0.0e+00
Identity = 2393/2432 (98.40%), Postives = 2399/2432 (98.64%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDD+DISISHEKNNP
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDFDISISHEKNNP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSK+DNSECSST+KYSFGFTWGSTKEC
Sbjct: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKRDNSECSSTNKYSFGFTWGSTKEC 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL
Sbjct: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR
Sbjct: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500
            KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE
Sbjct: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500

Query: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560
            ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN
Sbjct: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560

Query: 1561 VTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620
            VTQDDDM+EQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRH+WSQ
Sbjct: 1561 VTQDDDMIEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHVWSQ 1620

Query: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680
            MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL
Sbjct: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680

Query: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740
            LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG
Sbjct: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740

Query: 1741 EWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800
            EWTYSNAFNKN LPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP
Sbjct: 1741 EWTYSNAFNKNALPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800

Query: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860
            PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER
Sbjct: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860

Query: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920
            SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL
Sbjct: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920

Query: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980
            SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM
Sbjct: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980

Query: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040
            IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL
Sbjct: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040

Query: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100
            AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC
Sbjct: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100

Query: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160
            TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI
Sbjct: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160

Query: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220
            WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG
Sbjct: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220

Query: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280
            GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK
Sbjct: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280

Query: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------DNFVS 2340
            EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD       DNFVS
Sbjct: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQEDNFVS 2340

Query: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400
            ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY
Sbjct: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400

BLAST of Carg07225 vs. NCBI nr
Match: XP_023547929.1 (piezo-type mechanosensitive ion channel homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 4717.9 bits (12236), Expect = 0.0e+00
Identity = 2390/2432 (98.27%), Postives = 2396/2432 (98.52%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKVSSTPEWPGICSYISLILFY MLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTPEWPGICSYISLILFYTMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYTGT NNYCHDNPSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTKNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSK+DNSECSST+KYSFGFTWGSTKEC
Sbjct: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKRDNSECSSTNKYSFGFTWGSTKEC 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL
Sbjct: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR
Sbjct: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500
            KQSTSR+DDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE
Sbjct: 1441 KQSTSRIDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500

Query: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560
            ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN
Sbjct: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560

Query: 1561 VTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620
            VTQDDDM+EQ NTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ
Sbjct: 1561 VTQDDDMIEQSNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620

Query: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680
            MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL
Sbjct: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680

Query: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740
            LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG
Sbjct: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740

Query: 1741 EWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800
            EWTYSNAFNK+DLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP
Sbjct: 1741 EWTYSNAFNKSDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800

Query: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860
            PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER
Sbjct: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860

Query: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920
            SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL
Sbjct: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920

Query: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980
            SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM
Sbjct: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980

Query: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040
            IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL
Sbjct: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040

Query: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100
            AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC
Sbjct: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100

Query: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160
            TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLFFILICVI
Sbjct: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVI 2160

Query: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220
            WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG
Sbjct: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220

Query: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280
            GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK
Sbjct: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280

Query: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------DNFVS 2340
            EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD       DNFVS
Sbjct: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQEDNFVS 2340

Query: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400
            ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY
Sbjct: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400

BLAST of Carg07225 vs. NCBI nr
Match: XP_022992489.1 (piezo-type mechanosensitive ion channel homolog [Cucurbita maxima])

HSP 1 Score: 4682.9 bits (12145), Expect = 0.0e+00
Identity = 2373/2432 (97.57%), Postives = 2386/2432 (98.11%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVFWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADIFWYRIGFVQR DSCWVH FSFVDHLGSHLRVASCLLLPAIQIIVGIS+PSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRGDSCWVHFFSFVDHLGSHLRVASCLLLPAIQIIVGISNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKVSST EWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTSEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVGFIV
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLN+KIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNQKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEI MLAGFCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEITMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHER+IASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERQIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVER KRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLERVPGS LNKGKWDDPCPLFVSDEDDYDISISHEKNNP
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSALNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLDAGRLFVQQEGSDHILRPSFVS+QSQVPHSASSK+DNS CSSTSKYSFGF WGSTKEC
Sbjct: 961  SLDAGRLFVQQEGSDHILRPSFVSNQSQVPHSASSKRDNSRCSSTSKYSFGFIWGSTKEC 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL
Sbjct: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNA SPIPGNEFLR
Sbjct: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNA-SPIPGNEFLR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500
            KQSTSR+DDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE
Sbjct: 1441 KQSTSRIDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500

Query: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560
            ICEIDSQISDISLDLDRKRKH+SSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN
Sbjct: 1501 ICEIDSQISDISLDLDRKRKHKSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560

Query: 1561 VTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620
            VTQDDDM+EQ NTEDRVYDQIESQETRYTHFERSSSL SDKSSDPATMQLGRIFRHIWSQ
Sbjct: 1561 VTQDDDMIEQRNTEDRVYDQIESQETRYTHFERSSSLHSDKSSDPATMQLGRIFRHIWSQ 1620

Query: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680
            MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL
Sbjct: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680

Query: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740
            LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG
Sbjct: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740

Query: 1741 EWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800
            EWTYSNAFNK+DLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLT+GAESP
Sbjct: 1741 EWTYSNAFNKSDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTQGAESP 1800

Query: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860
            PYFIQVS+DVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER
Sbjct: 1801 PYFIQVSMDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860

Query: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920
            SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL
Sbjct: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920

Query: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980
            SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM
Sbjct: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980

Query: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040
            IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTY VT+YAWQMEPSNQHAGELALRAIFL
Sbjct: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYVVTEYAWQMEPSNQHAGELALRAIFL 2040

Query: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100
            AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC
Sbjct: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100

Query: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160
            TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLFFILICVI
Sbjct: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVI 2160

Query: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220
            WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG
Sbjct: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220

Query: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280
            GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK
Sbjct: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280

Query: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------DNFVS 2340
            EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD       DNFVS
Sbjct: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQEDNFVS 2340

Query: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400
            ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY
Sbjct: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400

BLAST of Carg07225 vs. NCBI nr
Match: XP_038899059.1 (piezo-type mechanosensitive ion channel homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 4367.8 bits (11327), Expect = 0.0e+00
Identity = 2220/2436 (91.13%), Postives = 2299/2436 (94.38%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGG LLPLLLLTAALINWSL+SLTDLL+FLFIQFNA KI                 
Sbjct: 1    MGSFLGGLLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRYLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLL+IFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            +ADIFWYRIG V R DSCWVH  SFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  SADIFWYRIGLVPRGDSCWVHFLSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFCIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+SST EWP ICSY+SLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSTSEWPEICSYVSLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC+S EDERSSND  S RE GETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDERSSNDTSSPREEGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNE+HFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEVHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QI LIS+VLDRE SLCREILLQLGLLGRDSMWEFLEI +LA FCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISDVLDRESSLCREILLQLGLLGRDSMWEFLEITLLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYT TT NYCHD+ SHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTATTINYCHDSHSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVER KRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF SFRIWLSRSIDL F+LG+NSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGFNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            Y+SSDEPE LEYGRLGF+KRFL WHSDKILFA+LFYASISPISAFGLLYLLGL +C+TLP
Sbjct: 781  YSSSDEPELLEYGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHS LS+FLGFRVF+PGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSHFLGFRVFQPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLER+PGSVLNKGKWDDPCPLFVS+EDDYDISIS+EKN P
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVSEEDDYDISISNEKNKP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLD+GRLF+QQEG DHI+RPSFVS QSQV HSASSK+DNSECSS+ KYSFGF WGSTKE 
Sbjct: 961  SLDSGRLFLQQEGLDHIVRPSFVSGQSQVSHSASSKRDNSECSSSRKYSFGFIWGSTKES 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDK+RII  RKERFE+QKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA+LNSVSL
Sbjct: 1021 HKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W MPS  GVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWLMPSNTGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHL GFSLSSTYRKM+S
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLPGFSLSSTYRKMMS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
             RKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 QRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEFEYVCRYLEAEQIGAIV EQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKR+RNLQVEKMKSEMLN QIQLHNMNSS DGNN  SP PGNE  R
Sbjct: 1381 WKTEQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNV-SPSPGNESFR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHSMEF 1500
            K+STSR+ DDAMT+DIEGT LG  EQ+IR DSSP PELQESLAN+R    TESRMHSME 
Sbjct: 1441 KRSTSRIHDDAMTTDIEGT-LGNAEQIIREDSSPHPELQESLANMRIGITTESRMHSMEL 1500

Query: 1501 SVPEICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560
             V EICE+DSQISD+SLDLDRKRKH+ SAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA
Sbjct: 1501 PVAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560

Query: 1561 SFLNVTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRH 1620
            SFLNVTQDDD+ EQ N EDRVYDQIESQETRY H ERSSSLQSDKSSDPA+MQLGRIFRH
Sbjct: 1561 SFLNVTQDDDINEQSNNEDRVYDQIESQETRYAHLERSSSLQSDKSSDPASMQLGRIFRH 1620

Query: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTE 1680
            IW+QMR+NNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWV+MLIYTE
Sbjct: 1621 IWAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTE 1680

Query: 1681 LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSIT 1740
            LYILLQYLYQIIIQHCGL INSDLLQELGFPTHRITSSFV+SSLPLFLVYLFTLLQSSIT
Sbjct: 1681 LYILLQYLYQIIIQHCGLNINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSIT 1740

Query: 1741 AKDGEWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKG 1800
            AKDGEW  SNA NKN +PR+ SLGHYGLTEKAYELL+LG KMMMFVLRSLCKYWKSLT+G
Sbjct: 1741 AKDGEWMSSNALNKNAVPRRQSLGHYGLTEKAYELLYLGRKMMMFVLRSLCKYWKSLTQG 1800

Query: 1801 AESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQ 1860
            AESPPYFIQVS+DVQ+WPE GIQPERIESGINQMLQIIHD RCKEQN R CPFSSRVHVQ
Sbjct: 1801 AESPPYFIQVSLDVQIWPEDGIQPERIESGINQMLQIIHDERCKEQNRRLCPFSSRVHVQ 1860

Query: 1861 SIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFP 1920
            SIERSKEN K+ALVVLEVVYSSPSTDTCAEW DSLTPAADV NEIRLAQ  GLVE TGFP
Sbjct: 1861 SIERSKENTKVALVVLEVVYSSPSTDTCAEWCDSLTPAADVANEIRLAQRNGLVEKTGFP 1920

Query: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFV 1980
            YQILSVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNN+EFLDVYQLEDQFPKEFV
Sbjct: 1921 YQILSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPKEFV 1980

Query: 1981 FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALR 2040
            FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVT+YAWQMEPSNQHAGELALR
Sbjct: 1981 FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALR 2040

Query: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100
            AIFLAKAVSL LQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL
Sbjct: 2041 AIFLAKAVSLTLQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100

Query: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFIL 2160
            DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLFFIL
Sbjct: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFIL 2160

Query: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMV 2220
            ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKT+SG+LTLYQTTLCEKI W+KLNANMV
Sbjct: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGKLTLYQTTLCEKISWDKLNANMV 2220

Query: 2221 LDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDR 2280
            LDPGGYLSPYN+DDIQLICCQADASVLWLVPGVVQSRFIHSLD NQDISI+FTW+LTRDR
Sbjct: 2221 LDPGGYLSPYNQDDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQDISISFTWILTRDR 2280

Query: 2281 PKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------D 2340
            PKGKEVV+YDR++ PRDLP+QSD+QKVLNGS+N F+IKNVY+RYFRVTGSGD       +
Sbjct: 2281 PKGKEVVKYDRVIDPRDLPSQSDVQKVLNGSLNGFRIKNVYQRYFRVTGSGDVRPLEQEE 2340

Query: 2341 NFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSI 2400
            +FVSADLILNR NY WWSFHDIQPINVS+CG FTGPVA VISEEIPPQGILGDTLSKFSI
Sbjct: 2341 SFVSADLILNRNNYKWWSFHDIQPINVSECGSFTGPVAFVISEEIPPQGILGDTLSKFSI 2400

BLAST of Carg07225 vs. ExPASy Swiss-Prot
Match: F4IN58 (Piezo-type mechanosensitive ion channel homolog OS=Arabidopsis thaliana OX=3702 GN=At2g48060/At2g48040/At2g48050 PE=2 SV=1)

HSP 1 Score: 3118.9 bits (8085), Expect = 0.0e+00
Identity = 1605/2443 (65.70%), Postives = 1927/2443 (78.88%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            M SFL GFLLP LLL AALINWS+IS  DL+ FL + + A +I                 
Sbjct: 1    MASFLVGFLLPSLLLAAALINWSVISFLDLIAFLLVHYIAPEIGYRFQRRHWLLWPIFIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    V +LVIW+  G  W   +  W ++IGFM ++SW++P+V+YFL +QLL+  VA
Sbjct: 61   SFAVFLAQVVYLVIWAALGQDWDTPDTGWMRVIGFMILKSWRNPTVMYFLALQLLTSLVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADI+  R GF + RD+ W H     +HLGSHLRVASCLLLPA+Q+ VGI +PSWVSLPF
Sbjct: 121  LADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRVASCLLLPAVQLAVGICNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSC GLVDWSLTSN  GLFRWWR L +YAGF I L+Y+YQLP+ +  M++W+A FIGL
Sbjct: 181  FIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNIVLLYLYQLPINFSDMIRWIASFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            F++S   E P ICS + L+LFYIMLS V+ DLE+MDFIMS  E+NL E+LLP K+SFFIR
Sbjct: 241  FRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDMDFIMSTSENNLAERLLPPKYSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            E R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL  LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLFALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFIL WA+STYIFNVAF+FLN K+GK                 
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFSFLNTKVGK----------------- 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
                  F LG+LVAL NLVNNSVFL +S+E  RSSN+        ETKVL+VATIAWGLR
Sbjct: 421  ------FGLGMLVALGNLVNNSVFLYLSEESSRSSNERSYVEADEETKVLVVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            K SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLSH ++RK+R+SLILL E+HFALLY+L
Sbjct: 481  KCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLSHNINRKIRKSLILLCEVHFALLYIL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            +I+L+SN L +EGS  RE+L QLGLL  +S W+FLEIA+LA FC IHNHGFE+LFSFSAI
Sbjct: 541  EIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFLEIALLACFCAIHNHGFEVLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPP+GFSIL+AGLNKSVLLSVY+  +++Y  DN ++ER IASFLS+IG+KFLSMY
Sbjct: 601  VRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYSQDNTTYERHIASFLSAIGQKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAF+TIL++V+ VKPNY+SFGY+FLLL+W+ GRQL E  KRRLWFPLKAYA++V
Sbjct: 661  RSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLWITGRQLFEETKRRLWFPLKAYAVLV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF S ++WLS  IDLYF+LGYNS+A  L N W+SLAVLIVMQLYSYERRQS 
Sbjct: 721  FMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPLLDNVWESLAVLIVMQLYSYERRQSG 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            +    +   L  G  GF +RFL+WH  KILFA+LFYAS+SPIS FG +YLLGL +C+T P
Sbjct: 781  HYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALFYASLSPISVFGFVYLLGLVICTTFP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            K S IPSK FL YTG +++AEYLFQ+WG QA MFPGQK++ LS++LG RV+EPGFWG+E 
Sbjct: 841  KSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFPGQKYAELSFYLGLRVYEPGFWGIES 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDED-DYDISISHEKNN 960
            GLRGKVL++AACTLQYNVFRWLER  G  + KGK+++PCPLFVS ED    +S S+ +N 
Sbjct: 901  GLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKYEEPCPLFVSAEDTTASVSSSNGENP 960

Query: 961  PSLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKE 1020
             S D   + ++Q  +     P F    +Q       K   SE  S+ K+SFG  WGS KE
Sbjct: 961  SSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLHPKTGGSESGSSRKFSFGHFWGSIKE 1020

Query: 1021 CHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVS 1080
             H+W++ RI+  +KERFE QK + KIY+KFW+EN+F+L+GLEI MI+LLLASFA+LN++S
Sbjct: 1021 SHRWNRRRILALKKERFETQKNLLKIYLKFWIENMFNLYGLEINMIALLLASFALLNAIS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCH 1140
            + Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +EY+A W +   SD   PS+  VHCH
Sbjct: 1081 MVYIALLAACVLLRRRVIQKLWPVVVFLFASILAIEYVATWNSFLPSD-QAPSETSVHCH 1140

Query: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMV 1200
            DCW I+ LYF+FC  CWLG+ VDD R L SYFVVFML+  KLRADH+S FS SSTY +M 
Sbjct: 1141 DCWSIAALYFKFCRECWLGVRVDDPRTLISYFVVFMLACFKLRADHISSFSESSTYHQMK 1200

Query: 1201 SHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            S RKN+FVWRDLSFETKSMWT+LDYLRLYCY HLLD+VL LILITGTLEYD+LHLGYLAF
Sbjct: 1201 SQRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLLDVVLILILITGTLEYDILHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVF R+RLEILKKKNK+F+FLR YNF LII SLAYQSP VG  + GKCET+ YI+E+IG
Sbjct: 1261 ALVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLAYQSPFVGNFNDGKCETVDYIYEVIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEF+YV RYLEAEQIGAIV EQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVSRYLEAEQIGAIVREQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFL 1440
            A KTEQLQ IR++EE KR+RNLQVEKMKSEMLN ++QLH MNS  +   AS   P  E L
Sbjct: 1381 ARKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLRVQLHRMNSDSNFGVAS---PRTEGL 1440

Query: 1441 RKQSTSRM--DDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFS 1500
            R++ +  +  D  A + +I+G +  K EQ I  DS  +P          T   + S E+S
Sbjct: 1441 RRRKSPYLIPDSGAASPEIDGVVHRKEEQPIDEDSQ-YPFEAHEFPVSTTPEALDSPEYS 1500

Query: 1501 V-PEICEIDSQISDIS-LDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNL 1560
                 CEI     D+  + ++R+RK +S  K NPL+SAVQ IGDGVSQVQ IGNQAV+NL
Sbjct: 1501 FGASPCEITEVQQDLDVMSMERERKQKSEGKENPLISAVQLIGDGVSQVQFIGNQAVNNL 1560

Query: 1561 ASFLNVT-QDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIF 1620
             +FLN++ ++ D  EQ + +D VYD++ESQ+ ++T FERS+SLQSD+SSD  + Q+GRIF
Sbjct: 1561 VNFLNISPENSDTNEQSSVDDEVYDEMESQKRKHTPFERSTSLQSDRSSDGTSFQIGRIF 1620

Query: 1621 RHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIY 1680
            RHIWS+M+SNND+VCYCCFI+ FLWNFSLLSMVYLAALF+YALCV+TGP ++FWVIML+Y
Sbjct: 1621 RHIWSRMQSNNDIVCYCCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMY 1680

Query: 1681 TELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSS 1740
            TE+YILLQYLYQIIIQHCGL+I++ LL ELGFPT RI SSFV+SSLPLFL+Y+FTL+QSS
Sbjct: 1681 TEIYILLQYLYQIIIQHCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSS 1740

Query: 1741 ITAKDGEWTYSNAF--NKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKS 1800
            IT KDG+W  S  F   +N    +  L    L+++  ++          V+RS+ +YW S
Sbjct: 1741 ITVKDGDWVPSADFTSRRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWIS 1800

Query: 1801 LTKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSR 1860
            LT+GAESPPYF+QV++DV +WPE GIQPER+E  +NQ+L+++H+ RC++ NP  CP+SSR
Sbjct: 1801 LTRGAESPPYFVQVTMDVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSR 1860

Query: 1861 VHVQSIERSKENAKMALVVLEVVYSSPSTD-TCAEWYDSLTPAADVTNEIRLAQHKGLVE 1920
            VHVQSIERS E    ALVVLEV Y+SP+   + AEWY SLTPA+DV  EIR AQH GL E
Sbjct: 1861 VHVQSIERSTETPNEALVVLEVEYASPTNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGE 1920

Query: 1921 NTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQF 1980
             TGFPY ILSVIGGGKR+ DLYAY+FGADLIVFF VAIFYQSVIKN +EF+DVYQLEDQF
Sbjct: 1921 GTGFPYPILSVIGGGKRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQF 1980

Query: 1981 PKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAG 2040
            P +FV ILM+IFFLIV+DR IYLCSFA GKV++YLF+L+LFTYAVT+YAW + P+ QHA 
Sbjct: 1981 PFDFVIILMVIFFLIVVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAA 2040

Query: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100
             LALR IFLAKA+SLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY GYRLYRALPFLYE
Sbjct: 2041 GLALRIIFLAKAMSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYE 2100

Query: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGIC 2160
            LRCVLDWSCT TSLTMYDWLKLED+NASLYLVKCD VLNR+ HK G KQT MTKCCNGIC
Sbjct: 2101 LRCVLDWSCTATSLTMYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGIC 2160

Query: 2161 LFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKL 2220
            LFFIL+CVIWAPMLMYSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+I  + +
Sbjct: 2161 LFFILLCVIWAPMLMYSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNI 2220

Query: 2221 NANMVLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWL 2280
            +  + L    +L  YN++DIQLICCQADASVLWLVP  V +RFI SLDW+ D+ I FTW+
Sbjct: 2221 DLGLDLGSQSFLPTYNKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWV 2280

Query: 2281 LTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD--- 2340
            L RDRPKGKE V+Y+R V P DLP +SDIQ VLNGSM+ F++ N+Y ++FRVTGSGD   
Sbjct: 2281 LNRDRPKGKETVKYERSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRS 2340

Query: 2341 ----DNFVSADLILNRENYD-WWSFHDIQPI-NVSKCGHFTGPVAIVISEEIPPQGILGD 2400
                 + VSAD+++N  N+  WWSFH+++   N+S C    GPVAI++SEE PPQG LGD
Sbjct: 2341 FEDQTDEVSADILINHANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGD 2400

BLAST of Carg07225 vs. ExPASy Swiss-Prot
Match: Q54S52 (Protein PIEZO homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282801 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.8e-39
Identity = 143/560 (25.54%), Postives = 251/560 (44.82%), Query Frame = 0

Query: 1906 DLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILMIIFFLIVLDR 1965
            D Y  +   D    FF+ IF Q+     +  +  +  ++  P++++ IL+  F +I+LDR
Sbjct: 2456 DYYMPLLFTDFACLFFLVIFPQNFTGIPSSDIAEFLEQNVIPRQYIVILLAQFGVIILDR 2515

Query: 1966 CIYL-----CSFAIGKVIFYLFNLVLFTY---AVTK-----YAWQMEPSNQHAGELALRA 2025
             IYL       F +  V+  L+++ LF Y    + K     Y W             L  
Sbjct: 2516 IIYLYKSVKAKFVLQIVLTVLYHVFLFFYFPDLIVKPFSFGYTW------------PLVV 2575

Query: 2026 IFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLD 2085
             +L K + L   A+QI YG P  S    +FL    S  + +GY LY+A+PF+YELR +LD
Sbjct: 2576 FYLMKCIYLYYSALQICYGYPILS--QNRFLMDGYSDFHNIGYALYKAIPFVYELRTLLD 2635

Query: 2086 WSCTTTSLTMYDWLKLEDINASLYLVKCDAV-LNRSQHKQGAKQTVMTKCCNGICLFFIL 2145
            W  T T++  YDWLK ED+ ++++ VKC    + R   ++G KQ    K   G+  F  L
Sbjct: 2636 WIATDTTMLFYDWLKFEDLYSTIFSVKCRLEWIKRQGRQKGHKQPKFEKFVTGVTFFIGL 2695

Query: 2146 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEK----IKWEKLN 2205
            + ++W P+++ SSG P +   P+ +   +V +   +  L + Q    E        ++ +
Sbjct: 2696 VILLWFPLIILSSGLPGSNIEPVNNIQIEVSVVGWNPFLKINQDLSLESGDGDSTMDQNS 2755

Query: 2206 ANMVLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLL 2265
             N + +   +L+  +R  IQ I     +  +W +    +++ I+ L  N  + I  ++ L
Sbjct: 2756 FNNLKEDYSFLTTDDRQGIQNISINTFSEEIWNLSPPAKAQLINYLLTNTSLQIEVSYTL 2815

Query: 2266 TRDRPKGKEVVR--------------YDRLVHPR-------DLPNQS------DIQKVLN 2325
            TR       +V               Y+ L   +       + PN++      D     N
Sbjct: 2816 TRSGGVNSVIVGSNSVQLKPDQELNFYNILTRVQSNNSNNSNNPNENSSSGSDDNNNNSN 2875

Query: 2326 GSM--NSFQIKNVYERYFRVTG---------SGDDNFVSADLI--LNRENYD-------- 2385
             ++  NSF ++ ++ ++ ++ G           + N ++ D +  +N  N++        
Sbjct: 2876 NNIGSNSFIVEGLFYKFIKLPGVSGNPIYPTDNNGNVLALDALFTMNSTNFNNSNQPPQY 2935

Query: 2386 WWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRFI 2400
            +W  +                    IS ++ P GI   TL    I GLY++ VL+VGRF+
Sbjct: 2936 FWQVNAYDSTKTYNISTLESIQFYTISSKL-PNGIT-STLVSAGIIGLYVSVVLSVGRFL 2995

BLAST of Carg07225 vs. ExPASy Swiss-Prot
Match: Q9H5I5 (Piezo-type mechanosensitive ion channel component 2 OS=Homo sapiens OX=9606 GN=PIEZO2 PE=1 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 8.9e-39
Identity = 199/864 (23.03%), Postives = 365/864 (42.25%), Query Frame = 0

Query: 1588 IFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIML 1647
            +F  +++ + + +++VCY   IL  + + S+++++    +F++A+     P   FW++ +
Sbjct: 1910 LFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAI 1969

Query: 1648 IYTELYILLQYLYQ--IIIQHCGLTINSDLLQELGFPTH-------RITSSFVISSLPLF 1707
            +YTE+ I+++Y +Q      +  + +N D       P H            +V+  L   
Sbjct: 1970 VYTEVAIVVKYFFQFGFFPWNKNVEVNKDK------PYHPPNIIGVEKKEGYVLYDLIQL 2029

Query: 1708 LVYLFTLLQSSITAKDGEW----TYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMM 1767
            L   F     SI    G W       +   + +   + SLGH             G +  
Sbjct: 2030 LALFF---HRSILKCHGLWDEDDMTESGMAREESDDELSLGH-------------GRRDS 2089

Query: 1768 MFVLRSLCKYWKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRC 1827
               L+S+     +L    ES    + V+     +PE      R  SG             
Sbjct: 2090 SDSLKSI-----NLAASVES----VHVT-----FPEQQTAVRRKRSG------------S 2149

Query: 1828 KEQNPRPCPFSSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTN 1887
              +  +   FSS    +    ++ +++    VL +           +  + L  A   T 
Sbjct: 2150 SSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTI 2209

Query: 1888 EIRLAQHKGLVENTGFPYQILSVIGGGKREIDLYAYVFGAD-----LIVFFFVAIFYQSV 1947
            +  L  +  + +   F Y ++          D+Y  +F AD     +IVF F A    S 
Sbjct: 2210 KKTLEIYVPIKQ---FFYNLIH--PEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSA 2269

Query: 1948 IKNNTEFLDVYQLEDQFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTY 2007
              + T  L     EDQ P  F+ +++I F  +V+DR +YL    +GKVIF     V+  +
Sbjct: 2270 AADITSSLS----EDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQ----VILVF 2329

Query: 2008 AVTKYAWQMEP---SNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSE 2067
             +  + + + P     + +  L  +  +  K V   L A QIR G P  + +   FLT  
Sbjct: 2330 GIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYP--TRVLGNFLTKS 2389

Query: 2068 VSRINYLGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC-DAVLN 2127
             + +N   ++ +R +PFL ELR V+DW  T T+L++  W+ +EDI A ++++KC      
Sbjct: 2390 YNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEK 2449

Query: 2128 RSQHKQGAKQTVMTKCCNGICLFFILICVIWAPMLMYS-SGNPTNVANPIKDASCQVDIK 2187
            R    +G K+  + K   G  +  +LIC++W P+L  S   +   V N   D S  + + 
Sbjct: 2450 RYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLG 2509

Query: 2188 TSSGRLTL----YQTTLCEKIKWEKLNANMVLDPGG--YLSPYNRDDIQLICCQADASVL 2247
                  T+     Q  + ++  + K       D G   +L  Y ++DI +   + +++ L
Sbjct: 2510 GYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSL 2569

Query: 2248 WLVPGVVQSRFIHS-LDWNQDISIAFTWLLTRDRPKG-KEVVRYDRLVHPRDLPNQSDIQ 2307
            W +    + + IH  LD N   S+ F+W + R+   G K  +  D+L  P     + +I 
Sbjct: 2570 WTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIA 2629

Query: 2308 KVLNG-----SMNSFQIKNVYERYFRVTGS----------GDDNFVSADLILNRE----- 2367
            K++ G     S     I+ +Y  Y +               ++NF+   +IL+R+     
Sbjct: 2630 KMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKY 2689

Query: 2368 NYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLYITFVLAVG 2401
            N +WW  +     N     +      +V ++++ P  +    L+ + I GLY + VL +G
Sbjct: 2690 NSEWWVLN--LTGNRIYNPNSQALELVVFNDKVSPPSL--GFLAGYGIMGLYASVVLVIG 2706

BLAST of Carg07225 vs. ExPASy Swiss-Prot
Match: Q8CD54 (Piezo-type mechanosensitive ion channel component 2 OS=Mus musculus OX=10090 GN=Piezo2 PE=1 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 4.4e-38
Identity = 204/862 (23.67%), Postives = 372/862 (43.16%), Query Frame = 0

Query: 1588 IFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIML 1647
            +F  +++ + + +++VCY   IL  + + S+++++    +F++A+     P   FW++ +
Sbjct: 1980 LFYAMYNTLVARSEMVCYFVIILNHMTSASIITLLLPILIFLWAMLSVPRPSRRFWMMAI 2039

Query: 1648 IYTELYILLQYLYQIIIQHCGLTINSDL---LQELGFPTHRI----TSSFVISSLPLFLV 1707
            +YTE+ I+++Y +Q          N DL    +   FP + I       +V+  L   L 
Sbjct: 2040 VYTEVAIVVKYFFQFGF----FPWNKDLEIYKERPYFPPNIIGVEKKEGYVLYDLIQLLA 2099

Query: 1708 YLFTLLQSSITAKDGEWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRS 1767
              F     SI    G W   +  + N   ++ S     L +        G +     L+S
Sbjct: 2100 LFF---HRSILKCHGLWDEDDIVDSN-TDKEGSDDELSLDQ--------GRRGSSDSLKS 2159

Query: 1768 LCKYWKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPR 1827
            +     +L    ES    + V+     +PE      R  S  +  +           +PR
Sbjct: 2160 I-----NLAASVES----VHVT-----FPEQPAAIRRKRSCSSSQI-----------SPR 2219

Query: 1828 PCPFSSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQ 1887
               FSS    +    ++ +++    VL +   S       +  + L  A   T +  L  
Sbjct: 2220 -SSFSSNRSKRGSTSTRNSSQKGSSVLSLKQKSKRELYMEKLQEHLIKAKAFTIKKTLQI 2279

Query: 1888 HKGLVENTGFPYQILSVIGGGKREIDLYAYVFGAD-----LIVFFFVAIFYQSVIKNNTE 1947
            +   V    F Y ++          D+Y  +F AD     +IVF F A    S   + T 
Sbjct: 2280 Y---VPIRQFFYDLIH--PDYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITS 2339

Query: 1948 FLDVYQLEDQFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYA 2007
             L     EDQ P  F+ +++I F  +V+DR +YL    +GKVIF     V+  + +  + 
Sbjct: 2340 SLS----EDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQ----VILVFGIHFWM 2399

Query: 2008 WQMEP---SNQHAGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY 2067
            + + P     + +  L  +  +  K V   L A QIR G P  + +   FLT   + +N 
Sbjct: 2400 FFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYP--TRVLGNFLTKSYNYVNL 2459

Query: 2068 LGYRLYRALPFLYELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKC-DAVLNRSQHKQ 2127
              ++ +R +PFL ELR V+DW  T T+L++  W+ +EDI A ++++KC      R    +
Sbjct: 2460 FLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPR 2519

Query: 2128 GAKQTVMTKCCNGICLFFILICVIWAPMLMYS-SGNPTNVANPIKDASCQVDIKTSSGRL 2187
            G K+    K   G  +  +LIC++W P+L  S   +   V N   D S  + +       
Sbjct: 2520 GQKKKKAVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIF 2579

Query: 2188 TL----YQTTLCEKIKWEKLNANMVLDPGG--YLSPYNRDDIQLICCQADASVLWLVPGV 2247
            T+     Q  + +  K+ +   +   + G   +L  Y R+D+ +   + +++ LW +   
Sbjct: 2580 TMSAQQSQLKVMDNSKYNEFLKSFGPNSGAMQFLENYEREDVTVAELEGNSNSLWTISPP 2639

Query: 2248 VQSRFIHSL-DWNQDISIAFTWLLTRDRPKG-KEVVRYDRLVHPRDLPNQSDIQKVLNG- 2307
             + + I  L D N   S+ F+W + R+   G K  +  D+L  P  +  ++ I K++ G 
Sbjct: 2640 SKQKMIQELTDPNSCFSVVFSWSIQRNMTLGAKAEIATDKLSFPLAVATRNSIAKMIAGN 2699

Query: 2308 ----SMNSFQIKNVYERYFRVTGS----------GDDNFVSADLILNRENY-----DWWS 2367
                S     I+ +Y  Y +               ++NF++  +IL R+N      +WW 
Sbjct: 2700 DTESSNTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILFRDNVTKSNSEWW- 2759

Query: 2368 FHDIQPINVSKCGHFT-GPVA---IVISEEIPPQGILGDTLSKFSIWGLYITFVLAVGRF 2401
                  +N++    F  G  A   +V ++++ P  +    L+ + I GLY + VL +G+F
Sbjct: 2760 -----VLNLTGSRIFNQGSQALELVVFNDKVSPPSL--GFLAGYGIMGLYASVVLVIGKF 2776

BLAST of Carg07225 vs. ExPASy Swiss-Prot
Match: M9MSG8 (Piezo-type mechanosensitive ion channel component OS=Drosophila melanogaster OX=7227 GN=Piezo PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 6.4e-37
Identity = 142/576 (24.65%), Postives = 255/576 (44.27%), Query Frame = 0

Query: 1896 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEF---LDVYQLEDQFPKEFVF 1955
            S++   +   D+YA +F  D + FF +   + +     TE    +  Y  E++ P  F+ 
Sbjct: 1924 SLVHKSRLATDVYALMFLCDFVNFFVLLFGFTAFGTQQTESDEGVQTYLAENKVPIPFLI 1983

Query: 1956 ILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEP--SNQHAGELAL 2015
            +L++ F LIV+DR +YL    + K+IF+ F+++     +  + + + P  + +    LA 
Sbjct: 1984 MLLVQFLLIVIDRALYLRKALVNKIIFHFFSVI----GIHIWMFFVVPAVTERTFNSLAP 2043

Query: 2016 RAIF-LAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRC 2075
              IF + K   + L + QI+ G P +  +   F T   S +N + +++Y  +PFLYELR 
Sbjct: 2044 PIIFYVIKCFYMLLSSYQIKSGYPKR--ILGNFFTKGFSMVNMIAFKVYMQIPFLYELRT 2103

Query: 2076 VLDWSCTTTSLTMYDWLKLEDINASLYLVKC------DAVLNRSQHKQGAKQTVMTKCCN 2135
            +LDW C  +++T++DWLK+EDI +++YL++C      D    R+Q K    + +M     
Sbjct: 2104 ILDWVCIDSTMTIFDWLKMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIM----G 2163

Query: 2136 GICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKI-- 2195
            G  +  I+IC IW P+ +++ GN    +N     S  + I        +Y T   + I  
Sbjct: 2164 GTIVLLIVIC-IWGPLCLFALGNAVGTSNVPFHVSLSIRIGPYD---PIYTTNNYDSIFE 2223

Query: 2196 ----KWEKLNANMVLDPGG--YLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDW 2255
                 + ++    + +     +++ Y+  D+  +    ++  LW +    + R ++ L  
Sbjct: 2224 INPEMYSQMTNAYIKEKQALTFIAGYDATDVAAVRLAGNSPSLWNIAPPDRQRLLNDLRN 2283

Query: 2256 NQDISIAFTWLLTRDRP-KG-------KEVVRYDR-------LVHPRDLPNQSDIQKVL- 2315
            N  +   F++ LTR  P KG       +  +  D        L+H   L    D++ +  
Sbjct: 2284 NHTLKARFSYSLTRKAPAKGLKENVGDEHAISLDESFEGRAALIHM--LSETHDVEPIYS 2343

Query: 2316 NGSMNS--------FQIKNVYERYFRVTGSGDDNFVSADLILNRENYD------------ 2375
            NG+ N           I  +  ++ +V  SGD   VS   +L+ ++YD            
Sbjct: 2344 NGTTNGTTPEVEEVVVIPGMIPKFIKVLNSGDAAVVS---VLSPKHYDYRPLVIKMHRDN 2403

Query: 2376 -----WWSFHD----------IQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSI 2401
                 WW   D          +     S C   +G V    +++  P       L+   I
Sbjct: 2404 ETNGLWWEIRDYCNDTFYNETLSKFAYSNC--TSGIVMYTFNDKKFPSTF--SFLTAGGI 2463

BLAST of Carg07225 vs. ExPASy TrEMBL
Match: A0A6J1GNI2 (piezo-type mechanosensitive ion channel homolog OS=Cucurbita moschata OX=3662 GN=LOC111456038 PE=3 SV=1)

HSP 1 Score: 4725.2 bits (12255), Expect = 0.0e+00
Identity = 2393/2432 (98.40%), Postives = 2399/2432 (98.64%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDD+DISISHEKNNP
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDFDISISHEKNNP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSK+DNSECSST+KYSFGFTWGSTKEC
Sbjct: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKRDNSECSSTNKYSFGFTWGSTKEC 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL
Sbjct: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR
Sbjct: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500
            KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE
Sbjct: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500

Query: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560
            ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN
Sbjct: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560

Query: 1561 VTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620
            VTQDDDM+EQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRH+WSQ
Sbjct: 1561 VTQDDDMIEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHVWSQ 1620

Query: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680
            MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL
Sbjct: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680

Query: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740
            LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG
Sbjct: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740

Query: 1741 EWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800
            EWTYSNAFNKN LPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP
Sbjct: 1741 EWTYSNAFNKNALPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800

Query: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860
            PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER
Sbjct: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860

Query: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920
            SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL
Sbjct: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920

Query: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980
            SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM
Sbjct: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980

Query: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040
            IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL
Sbjct: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040

Query: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100
            AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC
Sbjct: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100

Query: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160
            TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI
Sbjct: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160

Query: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220
            WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG
Sbjct: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220

Query: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280
            GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK
Sbjct: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280

Query: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------DNFVS 2340
            EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD       DNFVS
Sbjct: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQEDNFVS 2340

Query: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400
            ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY
Sbjct: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400

BLAST of Carg07225 vs. ExPASy TrEMBL
Match: A0A6J1JXP1 (piezo-type mechanosensitive ion channel homolog OS=Cucurbita maxima OX=3661 GN=LOC111488810 PE=3 SV=1)

HSP 1 Score: 4682.9 bits (12145), Expect = 0.0e+00
Identity = 2373/2432 (97.57%), Postives = 2386/2432 (98.11%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKIGFIFGRRFLVFWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA
Sbjct: 61   SSLVILAQVTFLVIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADIFWYRIGFVQR DSCWVH FSFVDHLGSHLRVASCLLLPAIQIIVGIS+PSWVSLPF
Sbjct: 121  AADIFWYRIGFVQRGDSCWVHFFSFVDHLGSHLRVASCLLLPAIQIIVGISNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FKVSST EWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKVSSTSEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVGFIV
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGFIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLN+KIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNQKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEI MLAGFCTIHNHGFEMLFSFSAI
Sbjct: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEITMLAGFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHER+IASFLSSIGEKFLSMY
Sbjct: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERQIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVER KRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP
Sbjct: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLERVPGS LNKGKWDDPCPLFVSDEDDYDISISHEKNNP
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSALNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
            SLDAGRLFVQQEGSDHILRPSFVS+QSQVPHSASSK+DNS CSSTSKYSFGF WGSTKEC
Sbjct: 961  SLDAGRLFVQQEGSDHILRPSFVSNQSQVPHSASSKRDNSRCSSTSKYSFGFIWGSTKEC 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL
Sbjct: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
            HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNA SPIPGNEFLR
Sbjct: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNA-SPIPGNEFLR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500
            KQSTSR+DDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE
Sbjct: 1441 KQSTSRIDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFSVPE 1500

Query: 1501 ICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560
            ICEIDSQISDISLDLDRKRKH+SSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN
Sbjct: 1501 ICEIDSQISDISLDLDRKRKHKSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLASFLN 1560

Query: 1561 VTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRHIWSQ 1620
            VTQDDDM+EQ NTEDRVYDQIESQETRYTHFERSSSL SDKSSDPATMQLGRIFRHIWSQ
Sbjct: 1561 VTQDDDMIEQRNTEDRVYDQIESQETRYTHFERSSSLHSDKSSDPATMQLGRIFRHIWSQ 1620

Query: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680
            MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL
Sbjct: 1621 MRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTELYIL 1680

Query: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740
            LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG
Sbjct: 1681 LQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSITAKDG 1740

Query: 1741 EWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKGAESP 1800
            EWTYSNAFNK+DLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLT+GAESP
Sbjct: 1741 EWTYSNAFNKSDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTQGAESP 1800

Query: 1801 PYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860
            PYFIQVS+DVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER
Sbjct: 1801 PYFIQVSMDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQSIER 1860

Query: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920
            SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL
Sbjct: 1861 SKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFPYQIL 1920

Query: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980
            SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM
Sbjct: 1921 SVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFVFILM 1980

Query: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALRAIFL 2040
            IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTY VT+YAWQMEPSNQHAGELALRAIFL
Sbjct: 1981 IIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYVVTEYAWQMEPSNQHAGELALRAIFL 2040

Query: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100
            AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC
Sbjct: 2041 AKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVLDWSC 2100

Query: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFILICVI 2160
            TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLFFILICVI
Sbjct: 2101 TTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTVMTKCCNGICLFFILICVI 2160

Query: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220
            WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG
Sbjct: 2161 WAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMVLDPG 2220

Query: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280
            GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK
Sbjct: 2221 GYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDRPKGK 2280

Query: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD-------DNFVS 2340
            EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD       DNFVS
Sbjct: 2281 EVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGDVRPLEQEDNFVS 2340

Query: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400
            ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY
Sbjct: 2341 ADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSIWGLY 2400

BLAST of Carg07225 vs. ExPASy TrEMBL
Match: A0A1S3BRG7 (LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel homolog OS=Cucumis melo OX=3656 GN=LOC103492709 PE=3 SV=1)

HSP 1 Score: 4344.3 bits (11266), Expect = 0.0e+00
Identity = 2202/2436 (90.39%), Postives = 2294/2436 (94.17%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSL+SLTDLL+FLFIQFNA KI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVSLTDLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFL+IWSLEGY+WSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLL+IFVA
Sbjct: 61   SSLVILAQVTFLLIWSLEGYKWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADIFWYRIG V R DSCW+H FSFV+HLGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFWYRIGLVPRGDSCWIHFFSFVNHLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPVEYPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVEYPSMLKWVAEFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+SS  EWP ICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR
Sbjct: 241  FKISSNSEWPEICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC+S EDE SSNDN S  EAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCLSGEDEHSSNDNSSPGEAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSH+V+RKMRQSLILLN +HFALLYLL
Sbjct: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHDVTRKMRQSLILLNVVHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            QI LISNVLDREGSLCREILLQLGLLGRDSMWEFLEIA+LA FCTIHNHGFEMLFSFSAI
Sbjct: 541  QIGLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIALLACFCTIHNHGFEMLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPPVGFSILRAGLNKSVLLSVYT TTNNYCHDNPSHERKIASFLSSIGEKFLSMY
Sbjct: 601  VEHTPSPPVGFSILRAGLNKSVLLSVYTATTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAFLTILLTVFFVKPNY+SFGYLFLLLVWMIGRQLVER KRRLWFPLKAYAIIV
Sbjct: 661  RSCGTYIAFLTILLTVFFVKPNYLSFGYLFLLLVWMIGRQLVERTKRRLWFPLKAYAIIV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF SFRIWLSRSIDL F+LGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR
Sbjct: 721  FVFIYCLSSFSSFRIWLSRSIDLDFYLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            Y+SSDEPE LE+GRLGF+KRFL WHSDKILFA+LFYASISPISAFGLLYLLGL +C+TLP
Sbjct: 781  YSSSDEPELLEFGRLGFIKRFLIWHSDKILFAALFYASISPISAFGLLYLLGLVICATLP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHS LSYFLG R F+PGFWGLEL
Sbjct: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSYLSYFLGLREFQPGFWGLEL 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDYDISISHEKNNP 960
            GLRGKVLIIAACTLQYNVFRWLER+PGSVLNKGKWDDPCPLFV++EDDYDISIS+EKN  
Sbjct: 901  GLRGKVLIIAACTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVTEEDDYDISISNEKNKS 960

Query: 961  SLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKEC 1020
             LD+GRLF QQEG DHI RPSFVS+Q+QVPHSASSK+DNSECSS  KYSFGF WGSTKE 
Sbjct: 961  PLDSGRLFEQQEGLDHIRRPSFVSAQNQVPHSASSKRDNSECSSNRKYSFGFIWGSTKES 1020

Query: 1021 HKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVSL 1080
            HKWDK+RII  RKERFE+QKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA+LNSVSL
Sbjct: 1021 HKWDKIRIISLRKERFELQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFALLNSVSL 1080

Query: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCHD 1140
            FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W+MPSKAGVHCHD
Sbjct: 1081 FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWHMPSKAGVHCHD 1140

Query: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMVS 1200
            CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTY KM+S
Sbjct: 1141 CWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYHKMMS 1200

Query: 1201 HRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260
             RKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA
Sbjct: 1201 QRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAFA 1260

Query: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIGF 1320
            LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSP VGEVSAGKCETMHYIFEMIGF
Sbjct: 1261 LVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPFVGEVSAGKCETMHYIFEMIGF 1320

Query: 1321 YKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKAA 1380
            YKYDYGFRITARSALVEIIIFMLVS+QSYMFS QEFEYVCRYLEAEQIGAIV EQEKK A
Sbjct: 1321 YKYDYGFRITARSALVEIIIFMLVSVQSYMFSSQEFEYVCRYLEAEQIGAIVREQEKKXA 1380

Query: 1381 WKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFLR 1440
            WKTEQLQHIRDSEE KR+RNLQVEKMKSEMLN QIQLHNMNSS DGNN  SP PGNE  R
Sbjct: 1381 WKTEQLQHIRDSEERKRQRNLQVEKMKSEMLNLQIQLHNMNSSVDGNNV-SPSPGNESFR 1440

Query: 1441 KQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHSMEF 1500
             +STSR+ DDAMT+DIEGT LGKVEQ+IR DSSP PELQESLA+LR    TESRMHSME 
Sbjct: 1441 MRSTSRIHDDAMTTDIEGT-LGKVEQIIREDSSPHPELQESLASLRAGLTTESRMHSMES 1500

Query: 1501 SVPEICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560
             V EICE+DSQISD+SLDLDRKRKH+ SAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA
Sbjct: 1501 PVAEICEVDSQISDVSLDLDRKRKHKGSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNLA 1560

Query: 1561 SFLNVTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIFRH 1620
            SFLNVTQDDD+ EQ  TED VYDQIE QETRY H ERSSSLQSD+SSDPA+MQLGRIFRH
Sbjct: 1561 SFLNVTQDDDINEQSKTEDGVYDQIEIQETRYAHLERSSSLQSDRSSDPASMQLGRIFRH 1620

Query: 1621 IWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIYTE 1680
            IW+QMR+NNDVVCYCCFILVFLWNFSLLSM YLAALFVYALCVNTGPGYMFWV+MLIYTE
Sbjct: 1621 IWAQMRTNNDVVCYCCFILVFLWNFSLLSMFYLAALFVYALCVNTGPGYMFWVVMLIYTE 1680

Query: 1681 LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSSIT 1740
            LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFV+SSLPLFLVYLFTLLQSSIT
Sbjct: 1681 LYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVVSSLPLFLVYLFTLLQSSIT 1740

Query: 1741 AKDGEWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSLTKG 1800
            AKDGEW YS+AFNKN LPRK S GHYGLT++AYELL++G KMMMFVLRSLCKYWKSLT+G
Sbjct: 1741 AKDGEWAYSSAFNKNALPRKQSFGHYGLTDRAYELLYIGRKMMMFVLRSLCKYWKSLTQG 1800

Query: 1801 AESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRVHVQ 1860
            AESPPYFIQVS+DVQ+WPE GIQPERIESGIN MLQIIH  RCKEQNPR CPFSSRVHVQ
Sbjct: 1801 AESPPYFIQVSLDVQIWPEDGIQPERIESGINHMLQIIHVERCKEQNPRLCPFSSRVHVQ 1860

Query: 1861 SIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENTGFP 1920
            SIERSKEN K+ALVVLEVVYSSPST+TCAEW DSLTPAADV NEIRLAQ  G VE+TGFP
Sbjct: 1861 SIERSKENTKVALVVLEVVYSSPSTNTCAEWCDSLTPAADVANEIRLAQRNGFVESTGFP 1920

Query: 1921 YQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPKEFV 1980
            YQILSVIGGGKREIDLYAYVFGAD+IVFF VAIFYQS+IKNN+EFLDVYQLEDQFPKEFV
Sbjct: 1921 YQILSVIGGGKREIDLYAYVFGADMIVFFLVAIFYQSIIKNNSEFLDVYQLEDQFPKEFV 1980

Query: 1981 FILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGELALR 2040
            F+LMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVT+YAWQMEPSNQHAGELALR
Sbjct: 1981 FVLMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGELALR 2040

Query: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELRCVL 2100
            AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTS+VSRINYLGYRLYRALPFLYELRCVL
Sbjct: 2041 AIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSDVSRINYLGYRLYRALPFLYELRCVL 2100

Query: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLFFIL 2160
            DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQT+MTKCCNGICLFFIL
Sbjct: 2101 DWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGDKQTIMTKCCNGICLFFIL 2160

Query: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNANMV 2220
            ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKT+SGRLTLYQTTLCEKI W+KLN NMV
Sbjct: 2161 ICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTTSGRLTLYQTTLCEKISWDKLNTNMV 2220

Query: 2221 LDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLTRDR 2280
            LDPGGYLSPYN+DDIQLICCQADASVLWLVP VVQS FIHSLD  QDI I+FTW+LTRDR
Sbjct: 2221 LDPGGYLSPYNQDDIQLICCQADASVLWLVPDVVQSGFIHSLDRKQDIIISFTWILTRDR 2280

Query: 2281 PKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSG-------DD 2340
            PKGKEVV+YDR++ PRDLPNQSD+QKVLNGSMN F+IKNVY+RYFRVTGSG       ++
Sbjct: 2281 PKGKEVVKYDRVIEPRDLPNQSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGEVRPLEQEE 2340

Query: 2341 NFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSKFSI 2400
            +FVSADLILNR NY+WWSFHDIQPINVS+CG FTGPVA VISEEIPPQGILGDTLSKFSI
Sbjct: 2341 SFVSADLILNRNNYEWWSFHDIQPINVSECGRFTGPVAFVISEEIPPQGILGDTLSKFSI 2400

BLAST of Carg07225 vs. ExPASy TrEMBL
Match: A0A6J1D7Y8 (piezo-type mechanosensitive ion channel homolog OS=Momordica charantia OX=3673 GN=LOC111018458 PE=3 SV=1)

HSP 1 Score: 4234.1 bits (10980), Expect = 0.0e+00
Identity = 2164/2443 (88.58%), Postives = 2268/2443 (92.84%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALIN SL+SL DLL+FLFIQFNA KI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINCSLVSLIDLLVFLFIQFNAHKIGFIFGRRNLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                      FL++WSLEGY  S+ANAWWAK+IGFMTIQSWKSPSVIYFLVVQLL+IFVA
Sbjct: 61   SSLVILSQGIFLLVWSLEGYNISLANAWWAKVIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADIF  RIG V R DSCWVHL SF D LGSHLRVASCLLLPAIQIIVGIS PSWVSLPF
Sbjct: 121  TADIFRDRIGLVPRGDSCWVHLLSFFDQLGSHLRVASCLLLPAIQIIVGISRPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGF IFLVYVYQLPV+YPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFSIFLVYVYQLPVQYPSMLKWVAEFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            FK+ ST EWP ICSY+SL LFYIMLSCVKCDLEEMDFI+SMRE+NLVEQLLPSKHSFFIR
Sbjct: 241  FKILSTSEWPEICSYVSLTLFYIMLSCVKCDLEEMDFILSMRENNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLV LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLF LHRLNGLLLVFILFWAISTYIFNVAFTFLNRK+GKDMDVWEM+GLWHYPIPG
Sbjct: 361  YAFPSLFHLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKLGKDMDVWEMMGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSN-----DNCSAREAGETKVLIVATI 480
            FFLLAQFCLGILVALVNLVNNSVFLC+S+EDE SSN     DN SAREAGETKVLIVATI
Sbjct: 421  FFLLAQFCLGILVALVNLVNNSVFLCLSNEDECSSNDNSNADNSSAREAGETKVLIVATI 480

Query: 481  AWGLRKSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFA 540
            AWGLRKSSR IVLTLIFLVAMKPGFIHAVYVVFFL+YLLSH+VSRKMRQSLILLNE+HF+
Sbjct: 481  AWGLRKSSRPIVLTLIFLVAMKPGFIHAVYVVFFLVYLLSHDVSRKMRQSLILLNEVHFS 540

Query: 541  LLYLLQINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLF 600
            LLYLLQINLIS+VLDREGS CREILLQLGLLGRDSMW+FLEIA+LA FC+IHNHGFEMLF
Sbjct: 541  LLYLLQINLISDVLDREGSFCREILLQLGLLGRDSMWDFLEIALLACFCSIHNHGFEMLF 600

Query: 601  SFSAIVQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEK 660
            SFSAIVQHTP PPVGFSILRAGLNKSVLLSVYT T N+Y HDN SHERKIASFLSSIGEK
Sbjct: 601  SFSAIVQHTPCPPVGFSILRAGLNKSVLLSVYTSTKNHYGHDNSSHERKIASFLSSIGEK 660

Query: 661  FLSMYRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKA 720
            FLSMYRSCGTYIAFLTILLTVFFVKPNYISFG+LFLLLVWMIGRQLV R KR+LWFPLKA
Sbjct: 661  FLSMYRSCGTYIAFLTILLTVFFVKPNYISFGFLFLLLVWMIGRQLVGRTKRQLWFPLKA 720

Query: 721  YAIIVFIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYE 780
            YAI VF+FIYCLSSFPSFRIWLSRSIDLYF+LGYNSEASSL NCWQSLAVLIVMQLYSYE
Sbjct: 721  YAITVFVFIYCLSSFPSFRIWLSRSIDLYFYLGYNSEASSLHNCWQSLAVLIVMQLYSYE 780

Query: 781  RRQSRYNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGL-G 840
            RRQSRY+SSDEPE LEYGR GF+KRFL WH DKILFA+LFYASISPISAFGLLYLLGL  
Sbjct: 781  RRQSRYSSSDEPELLEYGRFGFIKRFLIWHCDKILFAALFYASISPISAFGLLYLLGLVV 840

Query: 841  VCSTLPKISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPG 900
            VC+TLPK+SHIPSKLFLAYTGV++TAEYLFQMWGKQAGMFPGQKH +LS FLG RVFEPG
Sbjct: 841  VCATLPKMSHIPSKLFLAYTGVVLTAEYLFQMWGKQAGMFPGQKHYHLSRFLGLRVFEPG 900

Query: 901  FWGLELGLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDD-YDISI 960
            FWGLELGLRGKVLIIAACTLQYNVFRWLER+P SVLNKGKWDDPCPLFVS+E+D YDISI
Sbjct: 901  FWGLELGLRGKVLIIAACTLQYNVFRWLERMPSSVLNKGKWDDPCPLFVSEENDYYDISI 960

Query: 961  SHEKNNPSLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFT 1020
            S+E+  PSL++GRLF+  EGSDHI R SF S +SQVPHSASSK+DNSECSS+ KYSFGF 
Sbjct: 961  SNER-RPSLNSGRLFLHPEGSDHISRSSFASGRSQVPHSASSKRDNSECSSSKKYSFGFI 1020

Query: 1021 WGSTKECHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFA 1080
            WGSTKE HKWDKMRI+  RKERFEMQKIIFKIYMKFWMENLF+LFGLEITMISLLLASFA
Sbjct: 1021 WGSTKESHKWDKMRIMSLRKERFEMQKIIFKIYMKFWMENLFNLFGLEITMISLLLASFA 1080

Query: 1081 VLNSVSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSK 1140
            +LNSVS+FYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NMWNS+W+M SK
Sbjct: 1081 LLNSVSIFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMWNSNWHMLSK 1140

Query: 1141 AGVHCHDCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSS 1200
            AGVHCHDCWR SNLYF+FCLNCWLGLTVDDSRMLFSYF+VFMLSSLKLRADHLSGFSLSS
Sbjct: 1141 AGVHCHDCWRFSNLYFRFCLNCWLGLTVDDSRMLFSYFMVFMLSSLKLRADHLSGFSLSS 1200

Query: 1201 TYRKMVSHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLH 1260
            TYRKM+S RKNTFVWRDLSFETKSMWTI+DYLRLYCYCHLLDLVLALILITGTLEYDVLH
Sbjct: 1201 TYRKMMSQRKNTFVWRDLSFETKSMWTIIDYLRLYCYCHLLDLVLALILITGTLEYDVLH 1260

Query: 1261 LGYLAFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY 1320
            LGYLAFALVFFRLRLEILKKKNK+FKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY
Sbjct: 1261 LGYLAFALVFFRLRLEILKKKNKLFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHY 1320

Query: 1321 IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVH 1380
            IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEFEYVCRYLEAEQIGAIVH
Sbjct: 1321 IFEMIGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVH 1380

Query: 1381 EQEKKAAWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPI 1440
            EQEKKAAWKT QLQHIRDSEESKR+RNLQVEKMKSEMLN QIQLHNMNS  D NNAS   
Sbjct: 1381 EQEKKAAWKTSQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSFVDANNAS--- 1440

Query: 1441 PGNEFLRKQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TES 1500
            PG+E  RK+ TSR  DD MT+DIEGT LGKVEQ++R DSSP PELQES ANLR    TES
Sbjct: 1441 PGDESRRKR-TSRTHDDTMTTDIEGT-LGKVEQIVRQDSSPHPELQESFANLRTEITTES 1500

Query: 1501 RMHSMEFSVPEICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGN 1560
            R HSME  VPEICE+DSQISD+SLDLDRKRKHR SAKGNPL SAVQFIGDGVSQVQSIGN
Sbjct: 1501 RTHSMELPVPEICEVDSQISDVSLDLDRKRKHRDSAKGNPLKSAVQFIGDGVSQVQSIGN 1560

Query: 1561 QAVSNLASFLNVTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQ 1620
            QAVSNLASFLNVT DDDM E  NTEDRVYDQIESQET+Y+  ERSSSLQSDKSSDPA+MQ
Sbjct: 1561 QAVSNLASFLNVTLDDDMNEHTNTEDRVYDQIESQETKYSDLERSSSLQSDKSSDPASMQ 1620

Query: 1621 LGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWV 1680
            LGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWV
Sbjct: 1621 LGRIFRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWV 1680

Query: 1681 IMLIYTELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFT 1740
            +MLIYTELYILLQYLYQIIIQHCGLTIN D+LQELGFPTHRITSSFV+SSLPLFLVYLFT
Sbjct: 1681 VMLIYTELYILLQYLYQIIIQHCGLTINLDVLQELGFPTHRITSSFVVSSLPLFLVYLFT 1740

Query: 1741 LLQSSITAKDGEWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKY 1800
            LLQSSITAKDGEWT SNA NK+ LPRKHS  HYGLTE+AYELL+LG  MMMF+LRSLCKY
Sbjct: 1741 LLQSSITAKDGEWTSSNALNKSALPRKHSPAHYGLTERAYELLYLGRNMMMFLLRSLCKY 1800

Query: 1801 WKSLTKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPF 1860
            WKSLT+GAESPPYFIQVS+DVQVWPE GIQPERIESGINQ+LQIIH  RCK QNPR CPF
Sbjct: 1801 WKSLTRGAESPPYFIQVSMDVQVWPEDGIQPERIESGINQLLQIIHKDRCKVQNPRLCPF 1860

Query: 1861 SSRVHVQSIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGL 1920
             SRVHVQSIERSKEN+++ALVVLEVVYSSPS+D+CAEW+DSLTPAADV NEIRLAQ  GL
Sbjct: 1861 PSRVHVQSIERSKENSQVALVVLEVVYSSPSSDSCAEWHDSLTPAADVANEIRLAQRNGL 1920

Query: 1921 VENTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLED 1980
            VENTGFPYQI SVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNN+EFLDVYQLED
Sbjct: 1921 VENTGFPYQIFSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLED 1980

Query: 1981 QFPKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQH 2040
            QFPKEFVF+LMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVTKYAWQMEPSNQ 
Sbjct: 1981 QFPKEFVFMLMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTKYAWQMEPSNQQ 2040

Query: 2041 AGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFL 2100
             GELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFL
Sbjct: 2041 TGELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFL 2100

Query: 2101 YELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNG 2160
            YELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNG
Sbjct: 2101 YELRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNG 2160

Query: 2161 ICLFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWE 2220
            ICLFFILICVIW PMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKI W+
Sbjct: 2161 ICLFFILICVIWTPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKISWD 2220

Query: 2221 KLNANMVLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFT 2280
            KLN+NMVLDPGGYLSPYN++DIQLICCQADASVLWLVPGVVQSRFIHSLDW QDISI FT
Sbjct: 2221 KLNSNMVLDPGGYLSPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDWKQDISIDFT 2280

Query: 2281 WLLTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD- 2340
            W+LTR+RPKGKEVVRYDR V   DLP QSD+QKVLNGS NSF+IKNVY+RYFRVTGSGD 
Sbjct: 2281 WVLTRERPKGKEVVRYDRTVEAPDLPEQSDVQKVLNGSRNSFRIKNVYQRYFRVTGSGDV 2340

Query: 2341 ------DNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGD 2400
                  D++V+ADLILNR NY WWSFHDIQPIN+S+CG FTGPVA VISEEIPPQGILGD
Sbjct: 2341 RPMEQEDSYVTADLILNRANYKWWSFHDIQPINISECGSFTGPVAFVISEEIPPQGILGD 2400

BLAST of Carg07225 vs. ExPASy TrEMBL
Match: A0A6J1KQD8 (piezo-type mechanosensitive ion channel homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497741 PE=3 SV=1)

HSP 1 Score: 4199.0 bits (10889), Expect = 0.0e+00
Identity = 2145/2439 (87.95%), Postives = 2258/2439 (92.58%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            MGSFLGGFLLPLLLLTAALINWSL++L DLL+FLFIQFNA KI                 
Sbjct: 1    MGSFLGGFLLPLLLLTAALINWSLVALADLLVFLFIQFNAHKIGFIFGRRFLVLWPIIIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    VTFLVIWSLEGY WS+ANAWWAKLIGFMTIQSWKSPSVIYFLVVQLL+IFVA
Sbjct: 61   SSLVILSQVTFLVIWSLEGYNWSLANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLAIFVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
            AADI+WYRIG V R DSCWV   SFVD LGSHLRVASCLLLPAIQIIVGISHPSWVSLPF
Sbjct: 121  AADIYWYRIGLVPRGDSCWVQFSSFVDRLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVY YQLPV YPSMLKWVA FIGL
Sbjct: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYAYQLPVGYPSMLKWVAKFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
             K+SST EWP ICSY+SLILFYIMLSCVKCDLEEMDFIMS RESNLVEQLLPSKHSFFIR
Sbjct: 241  SKISSTSEWPEICSYVSLILFYIMLSCVKCDLEEMDFIMSTRESNLVEQLLPSKHSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            ELRSGV+HTNVLLRR+VFRTFTINFFTYGFPV+LV LSFWSFHFASLCAFGLLAYVG+IV
Sbjct: 301  ELRSGVRHTNVLLRRDVFRTFTINFFTYGFPVALVALSFWSFHFASLCAFGLLAYVGYIV 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            Y FPSLF+LHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDM VWEMVGLWHYPIPG
Sbjct: 361  YVFPSLFRLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMHVWEMVGLWHYPIPG 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
            FFLLAQF LGILVALVNLVNNSVFLC SDEDERSSN+N S REAGETKVLIVATIAWGLR
Sbjct: 421  FFLLAQFGLGILVALVNLVNNSVFLCSSDEDERSSNENSSVREAGETKVLIVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            KSSRAI+LTLIFLVAMKPG IHAVYVVFFLLYLLSH+V RKMRQ++ILLNE+HFALLYLL
Sbjct: 481  KSSRAILLTLIFLVAMKPGLIHAVYVVFFLLYLLSHDVGRKMRQAIILLNEVHFALLYLL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLG-RDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSA 600
            QINL+S+VL REGSLCREILLQLGLLG RDS WEFLEIA+LA FC+IHNHGFEMLFSFSA
Sbjct: 541  QINLVSDVLGREGSLCREILLQLGLLGRRDSTWEFLEIALLACFCSIHNHGFEMLFSFSA 600

Query: 601  IVQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSM 660
            +VQHTPSPPVGFSILRAGLNKSVLLSVYT T+NNYCHDNPSHERKIASFLSSIGEKFLSM
Sbjct: 601  VVQHTPSPPVGFSILRAGLNKSVLLSVYTATSNNYCHDNPSHERKIASFLSSIGEKFLSM 660

Query: 661  YRSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAII 720
            YRSCGTYIAFLTILLTV FVKPNYISFGYLFLLLVW+IGRQLVER KRRLWFPLKAYAII
Sbjct: 661  YRSCGTYIAFLTILLTVCFVKPNYISFGYLFLLLVWIIGRQLVERTKRRLWFPLKAYAII 720

Query: 721  VFIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQS 780
            VFIFIYC+SSF SFRIWLSRSIDL F+LGY+SEASSLQNCWQSLAV+IVMQLYSYERRQS
Sbjct: 721  VFIFIYCMSSFSSFRIWLSRSIDLNFYLGYDSEASSLQNCWQSLAVMIVMQLYSYERRQS 780

Query: 781  RYNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTL 840
            RY SSDEP  LEYG  GF+KRFL WH DKILFA+LFYASISPISAFGLLYLLGL VC+TL
Sbjct: 781  RYLSSDEPGLLEYG--GFIKRFLIWHCDKILFAALFYASISPISAFGLLYLLGLVVCATL 840

Query: 841  PKISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLE 900
            PK SHIPSKLFLAYTGVIMTAEYLFQMWG+QA MFPGQKH++LSYFLGFRVF PGFWGLE
Sbjct: 841  PKASHIPSKLFLAYTGVIMTAEYLFQMWGEQAEMFPGQKHAHLSYFLGFRVFRPGFWGLE 900

Query: 901  LGLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDEDDY--DISISHEK 960
            LGLRGKVL+IAA TLQYNVFRWLER+PGSVLNKGKWDDPCPLFVS+EDDY  DIS S+EK
Sbjct: 901  LGLRGKVLVIAASTLQYNVFRWLERMPGSVLNKGKWDDPCPLFVSEEDDYYDDISSSNEK 960

Query: 961  NNPSLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGST 1020
            N PS D+GR FV QE SDHI   SF S ++Q+PH ASSK++NSECSS+ K  FGF WGS 
Sbjct: 961  NKPSFDSGRSFVPQEVSDHISSSSFASGRAQMPHDASSKRENSECSSSKKSPFGFIWGSI 1020

Query: 1021 KECHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNS 1080
            KE HKWDK RI   RKERFEMQKIIF+IYMKFWMENLF+LFGLEITMISLLL SFA+LNS
Sbjct: 1021 KESHKWDKTRITSLRKERFEMQKIIFEIYMKFWMENLFYLFGLEITMISLLLTSFALLNS 1080

Query: 1081 VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVH 1140
            VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFW NM NS+W +PSKA VH
Sbjct: 1081 VSLFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWKNMMNSNWLIPSKADVH 1140

Query: 1141 CHDCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRK 1200
            CHDCWRISNLY++FCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRAD LSGFSLSSTY K
Sbjct: 1141 CHDCWRISNLYYKFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADRLSGFSLSSTYHK 1200

Query: 1201 MVSHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL 1260
            M+S RKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL
Sbjct: 1201 MMSQRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYL 1260

Query: 1261 AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM 1320
            AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM
Sbjct: 1261 AFALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEM 1320

Query: 1321 IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEK 1380
            IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEFEYVCRYLEAEQIGAIV EQEK
Sbjct: 1321 IGFYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFEYVCRYLEAEQIGAIVREQEK 1380

Query: 1381 KAAWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNE 1440
            KAAWKTEQLQHIRDSEESKR+RNLQVEKMKSEMLN QIQLHNMNSS +GNNA SP PG+E
Sbjct: 1381 KAAWKTEQLQHIRDSEESKRQRNLQVEKMKSEMLNLQIQLHNMNSSVNGNNA-SPSPGDE 1440

Query: 1441 FLRKQSTSRMDDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLR----TESRMHS 1500
             LRK+STSR+ DDAMT+DIEGT  GKVEQ+IR DSS   ELQESLANLR    TESR  S
Sbjct: 1441 SLRKRSTSRIHDDAMTTDIEGT-HGKVEQIIRDDSSSQLELQESLANLRTEITTESRTQS 1500

Query: 1501 MEFSVPEICEIDSQISDISLDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVS 1560
            ME  V +ICEI+SQISD+SLD DRKRKH+ S KGNPLMSAVQFIGDGVSQVQSIGNQAVS
Sbjct: 1501 MELPVADICEINSQISDVSLDSDRKRKHKGSTKGNPLMSAVQFIGDGVSQVQSIGNQAVS 1560

Query: 1561 NLASFLNVTQDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRI 1620
            NLA+FLNVTQDDDM EQ NTEDRVYDQIESQ+ RY   ERSSSLQSDKSSDPA+MQLGRI
Sbjct: 1561 NLATFLNVTQDDDMNEQTNTEDRVYDQIESQQPRYADLERSSSLQSDKSSDPASMQLGRI 1620

Query: 1621 FRHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLI 1680
            F +IWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVN GPGYMFWV+MLI
Sbjct: 1621 FHYIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNAGPGYMFWVVMLI 1680

Query: 1681 YTELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQS 1740
            YTELYILLQYLYQIIIQHCGL INSDLLQELGFPT+RITSSFV+SSLPLFLVYLFTLLQ 
Sbjct: 1681 YTELYILLQYLYQIIIQHCGLAINSDLLQELGFPTNRITSSFVVSSLPLFLVYLFTLLQR 1740

Query: 1741 SITAKDGEWTYSNAFNKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKSL 1800
            SITAKDGEWTY NAFNK+ LPRKHSLG YGLTE+AYE L++G KM+M +LRS  KYWKSL
Sbjct: 1741 SITAKDGEWTYLNAFNKSALPRKHSLGRYGLTERAYEFLYVGRKMVMVMLRSSWKYWKSL 1800

Query: 1801 TKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSRV 1860
            T+GAESPPYFIQVS+DVQ+WPE GIQPERIESGINQ+L+IIH+ RCK+QN R CPF SRV
Sbjct: 1801 TQGAESPPYFIQVSMDVQIWPEDGIQPERIESGINQLLRIIHEERCKKQNARLCPFYSRV 1860

Query: 1861 HVQSIERSKENAKMALVVLEVVYSSPSTDTCAEWYDSLTPAADVTNEIRLAQHKGLVENT 1920
            HVQSIERSKEN ++ALVVLEV YSSPS+DTC+EW DSLTPAADV NEIRLAQ  G VENT
Sbjct: 1861 HVQSIERSKENTEVALVVLEVEYSSPSSDTCSEWCDSLTPAADVANEIRLAQRNGFVENT 1920

Query: 1921 GFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQFPK 1980
            GFPY+ILSVIGGGKREIDLYAYVFGADL VFFFVAIFYQSVIKNN+EFLDVYQLEDQFPK
Sbjct: 1921 GFPYRILSVIGGGKREIDLYAYVFGADLTVFFFVAIFYQSVIKNNSEFLDVYQLEDQFPK 1980

Query: 1981 EFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAGEL 2040
            EFVF+LMIIFFLIVLDRCIYLCSFA GKVIFYLFNLVLFTYAVT+YAWQMEPSNQHAGEL
Sbjct: 1981 EFVFVLMIIFFLIVLDRCIYLCSFATGKVIFYLFNLVLFTYAVTEYAWQMEPSNQHAGEL 2040

Query: 2041 ALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELR 2100
            ALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELR
Sbjct: 2041 ALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYELR 2100

Query: 2101 CVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGICLF 2160
            CVLDWSCT+TSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQG KQTVMTKCCNGICLF
Sbjct: 2101 CVLDWSCTSTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGEKQTVMTKCCNGICLF 2160

Query: 2161 FILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKLNA 2220
            FIL+ VIWAPMLMYSSGNPTNV NPIKDASCQVDIKT+SGRLTLYQTTLCEKI W  LNA
Sbjct: 2161 FILLFVIWAPMLMYSSGNPTNVENPIKDASCQVDIKTTSGRLTLYQTTLCEKISWNNLNA 2220

Query: 2221 NMVLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWLLT 2280
            N+VLDPGGYL PYN++DIQLICCQADASVLWLVPGVVQSRFIHSLD NQ+IS++FTW+LT
Sbjct: 2221 NLVLDPGGYLGPYNKNDIQLICCQADASVLWLVPGVVQSRFIHSLDRNQNISVSFTWILT 2280

Query: 2281 RDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD----- 2340
            RDRPKGKEVV+YDR+V PRDLPN SD+QKVLNGSMN F+IKNVY+RYFRVTGSGD     
Sbjct: 2281 RDRPKGKEVVQYDRVVDPRDLPNPSDVQKVLNGSMNGFRIKNVYQRYFRVTGSGDVRPLE 2340

Query: 2341 --DNFVSADLILNRENYDWWSFHDIQPINVSKCGHFTGPVAIVISEEIPPQGILGDTLSK 2400
              D+FV+ADLILNRENY+WWSFHDIQPINVS+CGHFTGPVA VISEEIPPQGILGDTLSK
Sbjct: 2341 QEDSFVNADLILNRENYEWWSFHDIQPINVSECGHFTGPVAFVISEEIPPQGILGDTLSK 2400

BLAST of Carg07225 vs. TAIR 10
Match: AT2G48060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: shoot, sperm cell; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3595 (InterPro:IPR021999); Has 533 Blast hits to 341 proteins in 95 species: Archae - 2; Bacteria - 15; Metazoa - 318; Fungi - 0; Plants - 93; Viruses - 0; Other Eukaryotes - 105 (source: NCBI BLink). )

HSP 1 Score: 3118.9 bits (8085), Expect = 0.0e+00
Identity = 1605/2443 (65.70%), Postives = 1927/2443 (78.88%), Query Frame = 0

Query: 1    MGSFLGGFLLPLLLLTAALINWSLISLTDLLLFLFIQFNADKI----------------- 60
            M SFL GFLLP LLL AALINWS+IS  DL+ FL + + A +I                 
Sbjct: 1    MASFLVGFLLPSLLLAAALINWSVISFLDLIAFLLVHYIAPEIGYRFQRRHWLLWPIFIF 60

Query: 61   --------VTFLVIWSLEGYQWSIANAWWAKLIGFMTIQSWKSPSVIYFLVVQLLSIFVA 120
                    V +LVIW+  G  W   +  W ++IGFM ++SW++P+V+YFL +QLL+  VA
Sbjct: 61   SFAVFLAQVVYLVIWAALGQDWDTPDTGWMRVIGFMILKSWRNPTVMYFLALQLLTSLVA 120

Query: 121  AADIFWYRIGFVQRRDSCWVHLFSFVDHLGSHLRVASCLLLPAIQIIVGISHPSWVSLPF 180
             ADI+  R GF + RD+ W H     +HLGSHLRVASCLLLPA+Q+ VGI +PSWVSLPF
Sbjct: 121  LADIYSSRFGFARWRDTWWSHFSGIFEHLGSHLRVASCLLLPAVQLAVGICNPSWVSLPF 180

Query: 181  FIGSCVGLVDWSLTSNFLGLFRWWRPLQLYAGFIIFLVYVYQLPVEYPSMLKWVAGFIGL 240
            FIGSC GLVDWSLTSN  GLFRWWR L +YAGF I L+Y+YQLP+ +  M++W+A FIGL
Sbjct: 181  FIGSCAGLVDWSLTSNVSGLFRWWRVLYIYAGFNIVLLYLYQLPINFSDMIRWIASFIGL 240

Query: 241  FKVSSTPEWPGICSYISLILFYIMLSCVKCDLEEMDFIMSMRESNLVEQLLPSKHSFFIR 300
            F++S   E P ICS + L+LFYIMLS V+ DLE+MDFIMS  E+NL E+LLP K+SFFIR
Sbjct: 241  FRISLETEGPDICSGLFLVLFYIMLSYVRSDLEDMDFIMSTSENNLAERLLPPKYSFFIR 300

Query: 301  ELRSGVKHTNVLLRREVFRTFTINFFTYGFPVSLVVLSFWSFHFASLCAFGLLAYVGFIV 360
            E R+GV+HTNVLLR  VF+TF+INFFTYGFPVSL  LSFWSFHFASLCAFGLLAYVG+I+
Sbjct: 301  ESRAGVRHTNVLLRGAVFKTFSINFFTYGFPVSLFALSFWSFHFASLCAFGLLAYVGYII 360

Query: 361  YAFPSLFQLHRLNGLLLVFILFWAISTYIFNVAFTFLNRKIGKDMDVWEMVGLWHYPIPG 420
            YAFPSLFQLHRLNGLLLVFIL WA+STYIFNVAF+FLN K+GK                 
Sbjct: 361  YAFPSLFQLHRLNGLLLVFILLWAVSTYIFNVAFSFLNTKVGK----------------- 420

Query: 421  FFLLAQFCLGILVALVNLVNNSVFLCVSDEDERSSNDNCSAREAGETKVLIVATIAWGLR 480
                  F LG+LVAL NLVNNSVFL +S+E  RSSN+        ETKVL+VATIAWGLR
Sbjct: 421  ------FGLGMLVALGNLVNNSVFLYLSEESSRSSNERSYVEADEETKVLVVATIAWGLR 480

Query: 481  KSSRAIVLTLIFLVAMKPGFIHAVYVVFFLLYLLSHEVSRKMRQSLILLNEIHFALLYLL 540
            K SRAI+L LIFL+AMKPGF HAVYV+FFL+YLLSH ++RK+R+SLILL E+HFALLY+L
Sbjct: 481  KCSRAIMLALIFLIAMKPGFFHAVYVIFFLMYLLSHNINRKIRKSLILLCEVHFALLYIL 540

Query: 541  QINLISNVLDREGSLCREILLQLGLLGRDSMWEFLEIAMLAGFCTIHNHGFEMLFSFSAI 600
            +I+L+SN L +EGS  RE+L QLGLL  +S W+FLEIA+LA FC IHNHGFE+LFSFSAI
Sbjct: 541  EIDLVSNSLKQEGSASREVLFQLGLLRSESSWDFLEIALLACFCAIHNHGFEVLFSFSAI 600

Query: 601  VQHTPSPPVGFSILRAGLNKSVLLSVYTGTTNNYCHDNPSHERKIASFLSSIGEKFLSMY 660
            V+HTPSPP+GFSIL+AGLNKSVLLSVY+  +++Y  DN ++ER IASFLS+IG+KFLSMY
Sbjct: 601  VRHTPSPPIGFSILKAGLNKSVLLSVYSSPSSSYSQDNTTYERHIASFLSAIGQKFLSMY 660

Query: 661  RSCGTYIAFLTILLTVFFVKPNYISFGYLFLLLVWMIGRQLVERAKRRLWFPLKAYAIIV 720
            RSCGTYIAF+TIL++V+ VKPNY+SFGY+FLLL+W+ GRQL E  KRRLWFPLKAYA++V
Sbjct: 661  RSCGTYIAFITILISVYLVKPNYVSFGYIFLLLLWITGRQLFEETKRRLWFPLKAYAVLV 720

Query: 721  FIFIYCLSSFPSFRIWLSRSIDLYFFLGYNSEASSLQNCWQSLAVLIVMQLYSYERRQSR 780
            F+FIYCLSSF S ++WLS  IDLYF+LGYNS+A  L N W+SLAVLIVMQLYSYERRQS 
Sbjct: 721  FMFIYCLSSFVSLQLWLSGFIDLYFYLGYNSKAPLLDNVWESLAVLIVMQLYSYERRQSG 780

Query: 781  YNSSDEPEPLEYGRLGFVKRFLSWHSDKILFASLFYASISPISAFGLLYLLGLGVCSTLP 840
            +    +   L  G  GF +RFL+WH  KILFA+LFYAS+SPIS FG +YLLGL +C+T P
Sbjct: 781  HYIPGQSSLLHPGVFGFFERFLAWHGQKILFAALFYASLSPISVFGFVYLLGLVICTTFP 840

Query: 841  KISHIPSKLFLAYTGVIMTAEYLFQMWGKQAGMFPGQKHSNLSYFLGFRVFEPGFWGLEL 900
            K S IPSK FL YTG +++AEYLFQ+WG QA MFPGQK++ LS++LG RV+EPGFWG+E 
Sbjct: 841  KSSSIPSKSFLIYTGFLVSAEYLFQLWGMQAQMFPGQKYAELSFYLGLRVYEPGFWGIES 900

Query: 901  GLRGKVLIIAACTLQYNVFRWLERVPGSVLNKGKWDDPCPLFVSDED-DYDISISHEKNN 960
            GLRGKVL++AACTLQYNVFRWLER  G  + KGK+++PCPLFVS ED    +S S+ +N 
Sbjct: 901  GLRGKVLVVAACTLQYNVFRWLERTSGLTVIKGKYEEPCPLFVSAEDTTASVSSSNGENP 960

Query: 961  PSLDAGRLFVQQEGSDHILRPSFVSSQSQVPHSASSKQDNSECSSTSKYSFGFTWGSTKE 1020
             S D   + ++Q  +     P F    +Q       K   SE  S+ K+SFG  WGS KE
Sbjct: 961  SSTDHASISMKQGEATSNSWPFFSPRGNQGAGFLHPKTGGSESGSSRKFSFGHFWGSIKE 1020

Query: 1021 CHKWDKMRIIYWRKERFEMQKIIFKIYMKFWMENLFHLFGLEITMISLLLASFAVLNSVS 1080
             H+W++ RI+  +KERFE QK + KIY+KFW+EN+F+L+GLEI MI+LLLASFA+LN++S
Sbjct: 1021 SHRWNRRRILALKKERFETQKNLLKIYLKFWIENMFNLYGLEINMIALLLASFALLNAIS 1080

Query: 1081 LFYVGLLAACILLDRGIIRKLWPIFVFLFASILILEYIAFWMNMWNSDWNMPSKAGVHCH 1140
            + Y+ LLAAC+LL R +I+KLWP+ VFLFASIL +EY+A W +   SD   PS+  VHCH
Sbjct: 1081 MVYIALLAACVLLRRRVIQKLWPVVVFLFASILAIEYVATWNSFLPSD-QAPSETSVHCH 1140

Query: 1141 DCWRISNLYFQFCLNCWLGLTVDDSRMLFSYFVVFMLSSLKLRADHLSGFSLSSTYRKMV 1200
            DCW I+ LYF+FC  CWLG+ VDD R L SYFVVFML+  KLRADH+S FS SSTY +M 
Sbjct: 1141 DCWSIAALYFKFCRECWLGVRVDDPRTLISYFVVFMLACFKLRADHISSFSESSTYHQMK 1200

Query: 1201 SHRKNTFVWRDLSFETKSMWTILDYLRLYCYCHLLDLVLALILITGTLEYDVLHLGYLAF 1260
            S RKN+FVWRDLSFETKSMWT+LDYLRLYCY HLLD+VL LILITGTLEYD+LHLGYLAF
Sbjct: 1201 SQRKNSFVWRDLSFETKSMWTVLDYLRLYCYVHLLDVVLILILITGTLEYDILHLGYLAF 1260

Query: 1261 ALVFFRLRLEILKKKNKVFKFLRAYNFALIILSLAYQSPLVGEVSAGKCETMHYIFEMIG 1320
            ALVF R+RLEILKKKNK+F+FLR YNF LII SLAYQSP VG  + GKCET+ YI+E+IG
Sbjct: 1261 ALVFARMRLEILKKKNKIFRFLRVYNFVLIIFSLAYQSPFVGNFNDGKCETVDYIYEVIG 1320

Query: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSLQEFEYVCRYLEAEQIGAIVHEQEKKA 1380
            FYKYDYGFRITARSALVEIIIFMLVSLQSYMFS QEF+YV RYLEAEQIGAIV EQEKKA
Sbjct: 1321 FYKYDYGFRITARSALVEIIIFMLVSLQSYMFSSQEFDYVSRYLEAEQIGAIVREQEKKA 1380

Query: 1381 AWKTEQLQHIRDSEESKRKRNLQVEKMKSEMLNFQIQLHNMNSSFDGNNASSPIPGNEFL 1440
            A KTEQLQ IR++EE KR+RNLQVEKMKSEMLN ++QLH MNS  +   AS   P  E L
Sbjct: 1381 ARKTEQLQQIREAEEKKRQRNLQVEKMKSEMLNLRVQLHRMNSDSNFGVAS---PRTEGL 1440

Query: 1441 RKQSTSRM--DDDAMTSDIEGTLLGKVEQMIRTDSSPFPELQESLANLRTESRMHSMEFS 1500
            R++ +  +  D  A + +I+G +  K EQ I  DS  +P          T   + S E+S
Sbjct: 1441 RRRKSPYLIPDSGAASPEIDGVVHRKEEQPIDEDSQ-YPFEAHEFPVSTTPEALDSPEYS 1500

Query: 1501 V-PEICEIDSQISDIS-LDLDRKRKHRSSAKGNPLMSAVQFIGDGVSQVQSIGNQAVSNL 1560
                 CEI     D+  + ++R+RK +S  K NPL+SAVQ IGDGVSQVQ IGNQAV+NL
Sbjct: 1501 FGASPCEITEVQQDLDVMSMERERKQKSEGKENPLISAVQLIGDGVSQVQFIGNQAVNNL 1560

Query: 1561 ASFLNVT-QDDDMMEQGNTEDRVYDQIESQETRYTHFERSSSLQSDKSSDPATMQLGRIF 1620
             +FLN++ ++ D  EQ + +D VYD++ESQ+ ++T FERS+SLQSD+SSD  + Q+GRIF
Sbjct: 1561 VNFLNISPENSDTNEQSSVDDEVYDEMESQKRKHTPFERSTSLQSDRSSDGTSFQIGRIF 1620

Query: 1621 RHIWSQMRSNNDVVCYCCFILVFLWNFSLLSMVYLAALFVYALCVNTGPGYMFWVIMLIY 1680
            RHIWS+M+SNND+VCYCCFI+ FLWNFSLLSMVYLAALF+YALCV+TGP ++FWVIML+Y
Sbjct: 1621 RHIWSRMQSNNDIVCYCCFIIAFLWNFSLLSMVYLAALFLYALCVHTGPTHIFWVIMLMY 1680

Query: 1681 TELYILLQYLYQIIIQHCGLTINSDLLQELGFPTHRITSSFVISSLPLFLVYLFTLLQSS 1740
            TE+YILLQYLYQIIIQHCGL+I++ LL ELGFPT RI SSFV+SSLPLFL+Y+FTL+QSS
Sbjct: 1681 TEIYILLQYLYQIIIQHCGLSIDAPLLHELGFPTQRIKSSFVVSSLPLFLIYIFTLIQSS 1740

Query: 1741 ITAKDGEWTYSNAF--NKNDLPRKHSLGHYGLTEKAYELLHLGEKMMMFVLRSLCKYWKS 1800
            IT KDG+W  S  F   +N    +  L    L+++  ++          V+RS+ +YW S
Sbjct: 1741 ITVKDGDWVPSADFTSRRNARGSQKDLTRIRLSQRILDVFKKLRDSAKLVIRSIYRYWIS 1800

Query: 1801 LTKGAESPPYFIQVSIDVQVWPEYGIQPERIESGINQMLQIIHDGRCKEQNPRPCPFSSR 1860
            LT+GAESPPYF+QV++DV +WPE GIQPER+E  +NQ+L+++H+ RC++ NP  CP+SSR
Sbjct: 1801 LTRGAESPPYFVQVTMDVHMWPEDGIQPERVECRMNQLLRLVHNERCEKGNPDLCPYSSR 1860

Query: 1861 VHVQSIERSKENAKMALVVLEVVYSSPSTD-TCAEWYDSLTPAADVTNEIRLAQHKGLVE 1920
            VHVQSIERS E    ALVVLEV Y+SP+   + AEWY SLTPA+DV  EIR AQH GL E
Sbjct: 1861 VHVQSIERSTETPNEALVVLEVEYASPTNGCSSAEWYKSLTPASDVAKEIRKAQHSGLGE 1920

Query: 1921 NTGFPYQILSVIGGGKREIDLYAYVFGADLIVFFFVAIFYQSVIKNNTEFLDVYQLEDQF 1980
             TGFPY ILSVIGGGKR+ DLYAY+FGADLIVFF VAIFYQSVIKN +EF+DVYQLEDQF
Sbjct: 1921 GTGFPYPILSVIGGGKRDTDLYAYIFGADLIVFFLVAIFYQSVIKNKSEFIDVYQLEDQF 1980

Query: 1981 PKEFVFILMIIFFLIVLDRCIYLCSFAIGKVIFYLFNLVLFTYAVTKYAWQMEPSNQHAG 2040
            P +FV ILM+IFFLIV+DR IYLCSFA GKV++YLF+L+LFTYAVT+YAW + P+ QHA 
Sbjct: 1981 PFDFVIILMVIFFLIVVDRVIYLCSFATGKVVYYLFSLILFTYAVTEYAWSIYPTQQHAA 2040

Query: 2041 ELALRAIFLAKAVSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYLGYRLYRALPFLYE 2100
             LALR IFLAKA+SLALQAIQIRYGLPHKSTLYRQFLTSEVSRINY GYRLYRALPFLYE
Sbjct: 2041 GLALRIIFLAKAMSLALQAIQIRYGLPHKSTLYRQFLTSEVSRINYYGYRLYRALPFLYE 2100

Query: 2101 LRCVLDWSCTTTSLTMYDWLKLEDINASLYLVKCDAVLNRSQHKQGAKQTVMTKCCNGIC 2160
            LRCVLDWSCT TSLTMYDWLKLED+NASLYLVKCD VLNR+ HK G KQT MTKCCNGIC
Sbjct: 2101 LRCVLDWSCTATSLTMYDWLKLEDVNASLYLVKCDTVLNRATHKHGEKQTKMTKCCNGIC 2160

Query: 2161 LFFILICVIWAPMLMYSSGNPTNVANPIKDASCQVDIKTSSGRLTLYQTTLCEKIKWEKL 2220
            LFFIL+CVIWAPMLMYSSGNPTN+ANPIKDAS Q+D+KT  G+LTLYQTTLCE+I  + +
Sbjct: 2161 LFFILLCVIWAPMLMYSSGNPTNIANPIKDASVQIDLKTVGGKLTLYQTTLCERISGDNI 2220

Query: 2221 NANMVLDPGGYLSPYNRDDIQLICCQADASVLWLVPGVVQSRFIHSLDWNQDISIAFTWL 2280
            +  + L    +L  YN++DIQLICCQADASVLWLVP  V +RFI SLDW+ D+ I FTW+
Sbjct: 2221 DLGLDLGSQSFLPTYNKNDIQLICCQADASVLWLVPDTVVTRFIQSLDWDTDMDITFTWV 2280

Query: 2281 LTRDRPKGKEVVRYDRLVHPRDLPNQSDIQKVLNGSMNSFQIKNVYERYFRVTGSGD--- 2340
            L RDRPKGKE V+Y+R V P DLP +SDIQ VLNGSM+ F++ N+Y ++FRVTGSGD   
Sbjct: 2281 LNRDRPKGKETVKYERSVDPLDLPKRSDIQMVLNGSMDGFRVHNLYPKFFRVTGSGDVRS 2340

Query: 2341 ----DNFVSADLILNRENYD-WWSFHDIQPI-NVSKCGHFTGPVAIVISEEIPPQGILGD 2400
                 + VSAD+++N  N+  WWSFH+++   N+S C    GPVAI++SEE PPQG LGD
Sbjct: 2341 FEDQTDEVSADILINHANFKWWWSFHNLKASENISACEGMDGPVAIIMSEETPPQGFLGD 2400