Carg07216 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07216
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NEDD8-specific protease 1-like
Location	Carg_Chr17: 8357690 .. 8358364 (+)
RNA-Seq Expression	Carg07216
Synteny	Carg07216

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

mRNA sequence

ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

Coding sequence (CDS)

ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

Protein sequence

MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQINPI

Homology

[Close]

BLAST of Carg07216 vs. NCBI nr
Match: KAG7014407.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 451.1 bits (1159), Expect = 5.8e-123
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG
Sbjct: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQINPI 225
           RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQINPI
Sbjct: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQINPI 224

[Close]

BLAST of Carg07216 vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 434.9 bits (1117), Expect = 4.3e-118
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVI+G DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVS+LLG
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNLHEKKLIFFV+NDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 221
           RSKDEISG INVMRSVKSKYVESNMRSEMMQLIRRLKQEQ
Sbjct: 181 RSKDEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]

BLAST of Carg07216 vs. NCBI nr
Match: XP_023549404.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.3 bits (1074), Expect = 4.1e-113
Identity = 208/222 (93.69%), Postives = 214/222 (96.40%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVI+GSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVS+LLG
Sbjct: 1   MAAKFLTYGDVVIQGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNL EKKLIFFV+NDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLPEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSE AFTLYESV+GYINY+PAEFMECR PRQNNGYDCGLYVMETARVIYEHY NVDQF
Sbjct: 121 TMNSESAFTLYESVNGYINYEPAEFMECRTPRQNNGYDCGLYVMETARVIYEHYCNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           RSKDEISG INVMRSVKSKYVE NMRSEM+QLI  LKQEQIN
Sbjct: 181 RSKDEISGPINVMRSVKSKYVEFNMRSEMLQLIHGLKQEQIN 222

[Close]

BLAST of Carg07216 vs. NCBI nr
Match: KAG6575873.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 401.4 bits (1030), Expect = 5.2e-108
Identity = 196/198 (98.99%), Postives = 197/198 (99.49%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG
Sbjct: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 RSKDEISGTINVMRSVKS 199
           RSKDEISG INVMRSVK+
Sbjct: 181 RSKDEISGPINVMRSVKT 198

[Close]

BLAST of Carg07216 vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 329.7 bits (844), Expect = 1.9e-86
Identity = 159/218 (72.94%), Postives = 182/218 (83.49%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R SD+ SLE  NYLND IIDFYFAYL  +YQS++IL +PTS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N  DWD FI+T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ NGYDCGLYVME ARVIYE YFN DQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQ 219
           +++DEI G+ NV++ V  K+VESNMRSEM+QLI  L Q
Sbjct: 181 QNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGLNQ 218

[Close]

BLAST of Carg07216 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.8e-37
Identity = 86/221 (38.91%), Postives = 139/221 (62.90%), Query Frame = 0


Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  +LND +I+FY ++LS  + S  I  IP S+++ + N  
Sbjct: 9   KILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
           D +     ++ LNL +K L+   +NDNSNV  + GG HWSLLVY ++ N F+H+DS   +
Sbjct: 69  DTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYMGV 128

Query: 124 NSEPAFTLYESVSGYINYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNVDQFR 183
           N   A  L+++VS +++   A + EC   P+Q NGYDCG++++ TARVI E +F+    +
Sbjct: 129 NRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGGMK 188

Query: 184 SKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           ++DE+    NV  +V    + +++R E++ LI++L  E ++
Sbjct: 189 NRDEL-WFANVKETVPD--LVNHLREEILALIKKLMSESVS 225

[Close]

BLAST of Carg07216 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 65/175 (37.14%), Postives = 99/175 (56.57%), Query Frame = 0


Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  N+LND II F F Y +++     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
                    +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + ++ F
Sbjct: 126 NSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQNLF 179

[Close]

BLAST of Carg07216 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.9e-23
Identity = 64/175 (36.57%), Postives = 100/175 (57.14%), Query Frame = 0


Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  ++LND II F F Y + +     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
            +       +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SNPAEIAMFLEPLDLPNKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + +++F
Sbjct: 126 NSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFF 179

[Close]

BLAST of Carg07216 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.6e-22
Identity = 64/175 (36.57%), Postives = 97/175 (55.43%), Query Frame = 0


Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  ++LND II F F Y + +     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
                    +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + +  F
Sbjct: 126 NSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQSLF 179

[Close]

BLAST of Carg07216 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.8e-18
Identity = 54/174 (31.03%), Postives = 94/174 (54.02%), Query Frame = 0


Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL-- 65
           ++Y D  I  SD++ L+   +LND+II FY  +L    + N+   IP  V  L  + +  
Sbjct: 7   VSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNK-NKIPNQVLLLSPSVVFC 66

Query: 66  ------DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHY 125
                 + +I +   + L+L  K++IFF + +N + +   GG+HWSLL++ +  N F++Y
Sbjct: 67  CSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKFIYY 126

Query: 126 DSLSTMNSEPAFTLYESVSGYINYDPAE------FMECRAPRQNNGYDCGLYVM 165
           DS+++ NS  A  +       ++  P +       +  + P+Q NGYDCGLYV+
Sbjct: 127 DSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVL 179

[Close]

BLAST of Carg07216 vs. ExPASy TrEMBL
Match: A0A6J1GPM3 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.1e-118
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVI+G DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVS+LLG
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNLHEKKLIFFV+NDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 221
           RSKDEISG INVMRSVKSKYVESNMRSEMMQLIRRLKQEQ
Sbjct: 181 RSKDEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]

BLAST of Carg07216 vs. ExPASy TrEMBL
Match: A0A6J1JKF6 (NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 9.4e-87
Identity = 159/218 (72.94%), Postives = 182/218 (83.49%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R SD+ SLE  NYLND IIDFYFAYL  +YQS++IL +PTS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N  DWD FI+T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ NGYDCGLYVME ARVIYE YFN DQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQ 219
           +++DEI G+ NV++ V  K+VESNMRSEM+QLI  L Q
Sbjct: 181 QNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGLNQ 218

[Close]

BLAST of Carg07216 vs. ExPASy TrEMBL
Match: A0A6J1FTN2 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.3e-84
Identity = 153/212 (72.17%), Postives = 177/212 (83.49%), Query Frame = 0


Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R SD+ SLE  NYLND IIDFYFAYL  +YQS++IL I TS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N  DWD F++T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ N YDCGLYVME ARVIYEHYFN DQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQL 213
           +++DEI G+ NV++SV  K+VESNMRSE + +
Sbjct: 181 QNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

[Close]

BLAST of Carg07216 vs. ExPASy TrEMBL
Match: A0A151TN41 (Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 4.7e-38
Identity = 97/219 (44.29%), Postives = 135/219 (61.64%), Query Frame = 0


Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  YLND II+FYF+YLS+ + S + L +P S+++ +    
Sbjct: 9   KILSYNDVVLRRSDLDILSGPYYLNDRIIEFYFSYLSSCHPSQDTLLVPPSIAFWILKCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             +     IE L+L +K L+ F INDN +V  + GGSHWSLL Y R  NLF+H+DS  +M
Sbjct: 69  VAEALSDFIEPLHLSDKTLVIFPINDNDDVERAEGGSHWSLLAYYRNANLFVHHDSCRSM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY++VSGY+    +   A FMEC  +P Q NGYDCGLYV   AR+I   + N 
Sbjct: 129 NAAPAKQLYKAVSGYMGLSSSASEASFMECTDSPLQENGYDCGLYVTAIARIICSWHVN- 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
              ++ D      +V++   S  V ++MR E++ LIR L
Sbjct: 189 --HKNTDTNDLWFSVVKEQVSPSVVASMRGEILALIRDL 224

[Close]

BLAST of Carg07216 vs. ExPASy TrEMBL
Match: A0A1S3THK8 (NEDD8-specific protease 1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106755582 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 8.1e-38
Identity = 97/219 (44.29%), Postives = 134/219 (61.19%), Query Frame = 0


Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  YLND II+FYF+YLS+++ S ++L +P S+++ +    
Sbjct: 9   KILSYNDVVLRQSDLEILSGPYYLNDRIIEFYFSYLSSSHSSQDMLLVPPSIAFWIMQCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             D     +E L+L +K+L+ F INDN +V  + GGSHWSL+ YCR  N+F+H+DS   M
Sbjct: 69  VVDSLGDFLEPLHLPDKELVIFPINDNDDVDRAEGGSHWSLVAYCRNANVFVHHDSCRGM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMEC-RAPRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY++V+GY+    +   A F EC  +P Q+NGYDCGLYV  TAR I   + N 
Sbjct: 129 NAAPAKKLYKAVAGYMGSSGSATEASFQECTNSPLQDNGYDCGLYVTATARTICNWHANH 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
           +     D       V   V S  V S MR E++ LIR L
Sbjct: 189 NNTHGNDMWFSA--VKEQVTSSAVAS-MRGEILALIRDL 224

[Close]

BLAST of Carg07216 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.7e-38
Identity = 86/221 (38.91%), Postives = 139/221 (62.90%), Query Frame = 0


Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  +LND +I+FY ++LS  + S  I  IP S+++ + N  
Sbjct: 9   KILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
           D +     ++ LNL +K L+   +NDNSNV  + GG HWSLLVY ++ N F+H+DS   +
Sbjct: 69  DTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYMGV 128

Query: 124 NSEPAFTLYESVSGYINYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNVDQFR 183
           N   A  L+++VS +++   A + EC   P+Q NGYDCG++++ TARVI E +F+    +
Sbjct: 129 NRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGGMK 188

Query: 184 SKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           ++DE+    NV  +V    + +++R E++ LI++L  E ++
Sbjct: 189 NRDEL-WFANVKETVPD--LVNHLREEILALIKKLMSESVS 225

[Close]

BLAST of Carg07216 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.8e-06
Identity = 46/203 (22.66%), Postives = 74/203 (36.45%), Query Frame = 0


Query: 11  VVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNE--------------ILSIPTSVS 70
           VV+R  D+  L+ R ++NDTIIDFY  YL       E              + ++     
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLANLDKGTP 364

Query: 71  YLLGNTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHY 130
              G    +       ++++L EK  IF  IN +         HWSL++ C    L   +
Sbjct: 365 STCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS--------FHWSLVIICHPGELVPSH 424

Query: 131 DSLSTMNSE-------PAFTLYESV-----SGYINYDPAEFME----------------- 165
            +  + + E       P     +S+      G IN  P+   E                 
Sbjct: 425 VNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAP 484

[Close]

BLAST of Carg07216 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-05
Identity = 46/191 (24.08%), Postives = 71/191 (37.17%), Query Frame = 0


Query: 17  DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTI---- 76
           D+  L+   ++NDTIIDFY  YL    Q+ E        S+      D D   S+I    
Sbjct: 432 DVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGK 491

Query: 77  ----------ESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYC--------------- 136
                       +++  K  IF  +N N         HWSL+V C               
Sbjct: 492 AAFLRVRKWTRKVDMFGKDYIFVPVNYN--------LHWSLIVICHPGEVANRTDLDLDD 551

Query: 137 -RKRNLFMHYDSL-----------STMNSEPAFTLYESVSGYIN--YDPAEFMECRAPRQ 165
            +K    +H DS+            T   E     ++  S  I+  +    F+    P+Q
Sbjct: 552 SKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQ 611

[Close]

BLAST of Carg07216 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-05
Identity = 46/191 (24.08%), Postives = 71/191 (37.17%), Query Frame = 0


Query: 17  DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTI---- 76
           D+  L+   ++NDTIIDFY  YL    Q+ E        S+      D D   S+I    
Sbjct: 400 DVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGK 459

Query: 77  ----------ESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYC--------------- 136
                       +++  K  IF  +N N         HWSL+V C               
Sbjct: 460 AAFLRVRKWTRKVDMFGKDYIFVPVNYN--------LHWSLIVICHPGEVANRTDLDLDD 519

Query: 137 -RKRNLFMHYDSL-----------STMNSEPAFTLYESVSGYIN--YDPAEFMECRAPRQ 165
            +K    +H DS+            T   E     ++  S  I+  +    F+    P+Q
Sbjct: 520 SKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQ 579

The following BLAST results are available for this feature:

BLAST of Carg07216 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014407.1	5.8e-123	100.00	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022953908.1	4.3e-118	97.73	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_023549404.1	4.1e-113	93.69	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6575873.1	5.2e-108	98.99	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022988745.1	1.9e-86	72.94	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]

BLAST of Carg07216 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	3.8e-37	38.91	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q5FVJ8	1.1e-23	37.14	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q96LD8	1.9e-23	36.57	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q9D2Z4	1.6e-22	36.57	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q54XR2	6.8e-18	31.03	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Carg07216 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPM3	2.1e-118	97.73	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=...	[more]
A0A6J1JKF6	9.4e-87	72.94	NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 ...	[more]
A0A6J1FTN2	3.3e-84	72.17	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=...	[more]
A0A151TN41	4.7e-38	44.29	Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=3 SV=1	[more]
A0A1S3THK8	8.1e-38	44.29	NEDD8-specific protease 1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106755582 ...	[more]

BLAST of Carg07216 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	2.7e-38	38.91	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	2.8e-06	22.66	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	8.9e-05	24.08	Cysteine proteinases superfamily protein	[more]
AT1G09730.2	8.9e-05	24.08	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 76..172 e-value: 8.7E-12 score: 45.3
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 11..170 score: 16.498325
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 1..219 e-value: 4.4E-56 score: 192.0
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 4..219
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 4..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07216-RA	Carg07216-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity