Carg07158 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07158
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectin lyase-like superfamily protein
LocationCarg_Chr17: 8068514 .. 8070196 (+)
RNA-Seq ExpressionCarg07158
SyntenyCarg07158
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGCCTTCATGAAGGCGTGGGTAGAAGCATGCCATTCGGGCGGTCCGGCGAAGGTTGTTTTCCCACCAGGGAAGTTTCTAACAGGGCCGTTGGTGTATGCCGGGCCATGCGATGCACCAATGACCGTTGAAATTCAAGGCACTGTCTTGGCAACCACTGATGTTACTGAATATTCATCTCCAGTATGGATTCTATTTGAATCCATCACTGGTTTGAATCTCATTGGTAATGGAACCTTTGACGGTCAAGGTCAAGAAACATGGATGTATAATGATTGTCGCGACAACCCTAGTTGCATTATGCCCCCAACCGTAAGCATTCAATTTTATGTTTAATAGATCAATTAACTCCGTTTAAAATTATTTAATTATTATGAATTTGATGCAGTCATTGAAGTTCAACAAGGTGACCAATGGATTGATGGAAGGGATTTCTTCACTCAACAGCAAAGGCTTCCATATATTCTTGGTTTTAAGCCACCATCTTAGGGTTAATAATCTGAACATAATAGCTCCGGATCAGAGTCCTAACACGGATGGAATTCACATCAGCCAAACGGATAATGTTACCATTACAAGTTCCATCATAGGCACCGGAGACGACTGTGTTTCAATTGGCCATGGCTCTGTCAATGTCACTGTTTCCAACATCAAGTGTGGCCCAGGACATGGCATCAGGTATATAATAGGAGGGTTCTCACTTGTGTAGTAGGTTTGAAGTTCTAGAAGTTTTAAAGGTCGGTTTGAAATGACTTTCTAGAAAACCTTAAACCCTAAATTTAGTTTACAAATTTGTGATAACAATATCAATATCAATATCACCGTTGCCAACATCCCGTGTGGTTGCCAACATCCCGTGTGGTTGCCAACATCCCGTGTGGTTGCCAACATCCCGTGTGGCCCAGGACATGACATCAGGTATATGATATGTGAGCGTCTTGGCTCGTTTTAGAAGTTTAAGGTAGGTTTGAAATGACTTTTCAGAGTTTCCAAAAAAAATACTAAATCCTAAATCAATATCACTGTTGGCAACGTCATGGGCGTTTTGGCTCGTGTCGGTTTGAAGTTCAAATGTCTATTTGAAATGACTCTCTACAAAAAAAAAAAAAACCCTAAACCCTAAATTTAAATTCGATTTTGCAGCGTGGGAAGTCTAGGAAAATATAAAGATGAGAAGGATGTAAGTGGAATTTTTGTATCAAATTGCACACTAAAGAACACCACATTTGGAGTAAGGATCAAGACATGGGCAGATTCTCCTCCAAGCCAAGCTACCGGAATCCATTTCCAAGACATTGTCTTAGATTCAGTCAAAAATCCAATCATTATAGATCAATTTTACGGTTCGAAGAAGAACAAAAAGGTAATAAGCAACTAAGACTATGTTCGAGAGCGATAACGATAACGATCATGACCGTGATAATATAGTTTATGCTTCTCATGCAGCCATCAAAGGTGAAGATCAGCGACGTTTCTTACAAGAACATTCGGGGCACGACTATATCCGTCGTCCCCGTTTCATTACAGTGCAGCTCAGCCATCCCGTGCGATGGCATCACATTGGAAGATATCGATTTGGCCTTTATTGGGTCGGATCGGAAAAAGGCGTTGATCGGGAATTCATGTTTGAATGCCAAATTTAAGGCCACAGGAAAACAGAACCCACCAGCTTGTGCATAG

mRNA sequence

ATGCAGGCCTTCATGAAGGCGTGGGTAGAAGCATGCCATTCGGGCGGTCCGGCGAAGGTTGTTTTCCCACCAGGGAAGTTTCTAACAGGGCCGTTGGTGTATGCCGGGCCATGCGATGCACCAATGACCGTTGAAATTCAAGGCACTGTCTTGGCAACCACTGATGTTACTGAATATTCATCTCCAGTATGGATTCTATTTGAATCCATCACTGGTTTGAATCTCATTGGTAATGGAACCTTTGACGGTCAAGGTCAAGAAACATGGATGTATAATGATTGTCGCGACAACCCTAGTTGCATTATGCCCCCAACCTCATTGAAGTTCAACAAGGTGACCAATGGATTGATGGAAGGGATTTCTTCACTCAACAGCAAAGGCTTCCATATATTCTTGGTTTTAAGCCACCATCTTAGGGTTAATAATCTGAACATAATAGCTCCGGATCAGAGTCCTAACACGGATGGAATTCACATCAGCCAAACGGATAATGTTACCATTACAAGTTCCATCATAGGCACCGGAGACGACTGTGTTTCAATTGGCCATGGCTCTGTCAATGTCACTGTTTCCAACATCAAGTGTGGCCCAGGACATGGCATCAGCGTGGGAAGTCTAGGAAAATATAAAGATGAGAAGGATGTAAGTGGAATTTTTGTATCAAATTGCACACTAAAGAACACCACATTTGGAGTAAGGATCAAGACATGGGCAGATTCTCCTCCAAGCCAAGCTACCGGAATCCATTTCCAAGACATTGTCTTAGATTCAGTCAAAAATCCAATCATTATAGATCAATTTTACGGTTCGAAGAAGAACAAAAAGCCATCAAAGGTGAAGATCAGCGACGTTTCTTACAAGAACATTCGGGGCACGACTATATCCGTCGTCCCCGTTTCATTACAGTGCAGCTCAGCCATCCCGTGCGATGGCATCACATTGGAAGATATCGATTTGGCCTTTATTGGGTCGGATCGGAAAAAGGCGTTGATCGGGAATTCATGTTTGAATGCCAAATTTAAGGCCACAGGAAAACAGAACCCACCAGCTTGTGCATAG

Coding sequence (CDS)

ATGCAGGCCTTCATGAAGGCGTGGGTAGAAGCATGCCATTCGGGCGGTCCGGCGAAGGTTGTTTTCCCACCAGGGAAGTTTCTAACAGGGCCGTTGGTGTATGCCGGGCCATGCGATGCACCAATGACCGTTGAAATTCAAGGCACTGTCTTGGCAACCACTGATGTTACTGAATATTCATCTCCAGTATGGATTCTATTTGAATCCATCACTGGTTTGAATCTCATTGGTAATGGAACCTTTGACGGTCAAGGTCAAGAAACATGGATGTATAATGATTGTCGCGACAACCCTAGTTGCATTATGCCCCCAACCTCATTGAAGTTCAACAAGGTGACCAATGGATTGATGGAAGGGATTTCTTCACTCAACAGCAAAGGCTTCCATATATTCTTGGTTTTAAGCCACCATCTTAGGGTTAATAATCTGAACATAATAGCTCCGGATCAGAGTCCTAACACGGATGGAATTCACATCAGCCAAACGGATAATGTTACCATTACAAGTTCCATCATAGGCACCGGAGACGACTGTGTTTCAATTGGCCATGGCTCTGTCAATGTCACTGTTTCCAACATCAAGTGTGGCCCAGGACATGGCATCAGCGTGGGAAGTCTAGGAAAATATAAAGATGAGAAGGATGTAAGTGGAATTTTTGTATCAAATTGCACACTAAAGAACACCACATTTGGAGTAAGGATCAAGACATGGGCAGATTCTCCTCCAAGCCAAGCTACCGGAATCCATTTCCAAGACATTGTCTTAGATTCAGTCAAAAATCCAATCATTATAGATCAATTTTACGGTTCGAAGAAGAACAAAAAGCCATCAAAGGTGAAGATCAGCGACGTTTCTTACAAGAACATTCGGGGCACGACTATATCCGTCGTCCCCGTTTCATTACAGTGCAGCTCAGCCATCCCGTGCGATGGCATCACATTGGAAGATATCGATTTGGCCTTTATTGGGTCGGATCGGAAAAAGGCGTTGATCGGGAATTCATGTTTGAATGCCAAATTTAAGGCCACAGGAAAACAGAACCCACCAGCTTGTGCATAG

Protein sequence

MQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSSPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGISSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSIGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSPPSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA
Homology
BLAST of Carg07158 vs. NCBI nr
Match: KAG7014349.1 (Exopolygalacturonase-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.9 bits (1857), Expect = 1.0e-203
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYS 60
           MQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYS
Sbjct: 1   MQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYS 60

Query: 61  SPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGI 120
           SPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGI
Sbjct: 61  SPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGI 120

Query: 121 SSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVS 180
           SSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVS
Sbjct: 121 SSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVS 180

Query: 181 IGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADS 240
           IGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADS
Sbjct: 181 IGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADS 240

Query: 241 PPSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVS 300
           PPSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVS
Sbjct: 241 PPSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVS 300

Query: 301 LQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
           LQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA
Sbjct: 301 LQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 352

BLAST of Carg07158 vs. NCBI nr
Match: XP_022953710.1 (exopolygalacturonase clone GBGE184 [Cucurbita moschata])

HSP 1 Score: 714.9 bits (1844), Expect = 3.4e-202
Identity = 347/351 (98.86%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTD+TEYSS
Sbjct: 88  QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDITEYSS 147

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS
Sbjct: 148 PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 207

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI
Sbjct: 208 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 267

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP
Sbjct: 268 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 327

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           PSQATGIHFQDIVLDSVK+PIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL
Sbjct: 328 PSQATGIHFQDIVLDSVKSPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 387

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
           QCSSA+PCDGITLEDIDLAFIGSDRKKAL+GNSCLNAKFKATGKQNPPACA
Sbjct: 388 QCSSAVPCDGITLEDIDLAFIGSDRKKALVGNSCLNAKFKATGKQNPPACA 438

BLAST of Carg07158 vs. NCBI nr
Match: XP_023549399.1 (exopolygalacturonase clone GBGE184-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.0 bits (1782), Expect = 5.2e-195
Identity = 337/351 (96.01%), Postives = 343/351 (97.72%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTD+TEYSS
Sbjct: 67  QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDITEYSS 126

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPP SLKFNKVTNGLMEGIS
Sbjct: 127 PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPASLKFNKVTNGLMEGIS 186

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           SLNSKGFH+FLVLSHHLRVNNLNIIAPD SPNTDGIHIS     +ITSSIIGTGDDCVSI
Sbjct: 187 SLNSKGFHVFLVLSHHLRVNNLNIIAPDHSPNTDGIHIS-----SITSSIIGTGDDCVSI 246

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS NVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP
Sbjct: 247 GHGSGNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 306

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNI+GTTISVVPVSL
Sbjct: 307 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIQGTTISVVPVSL 366

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
           QCSSA+PCDGITLEDIDLAFIGSDRKKAL+GNSCLNAKFKATGKQNPPACA
Sbjct: 367 QCSSAVPCDGITLEDIDLAFIGSDRKKALVGNSCLNAKFKATGKQNPPACA 412

BLAST of Carg07158 vs. NCBI nr
Match: XP_038899827.1 (exopolygalacturonase clone GBGE184 [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.8e-159
Identity = 275/350 (78.57%), Postives = 304/350 (86.86%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+ WV ACHSGGPAKVVFP G FLTGPLVYAGPCD PMTVEIQGTV ATTD+TEYSS
Sbjct: 82  QAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSS 141

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
             WILFESITGL+LIG+GTFDGQG ETWMYNDCR +  C++PPTS+KFNKVTNGLMEGIS
Sbjct: 142 AEWILFESITGLSLIGSGTFDGQGAETWMYNDCRKHSRCVLPPTSIKFNKVTNGLMEGIS 201

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           SLNSK FHIF+VLSH+++VNNL+I AP  SPNTDGIHISQTD V IT+SIIGTGDDCVSI
Sbjct: 202 SLNSKAFHIFVVLSHNIKVNNLHITAPGNSPNTDGIHISQTDVVNITNSIIGTGDDCVSI 261

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+TVSN+ CGPGHGISVGSLGKY DEK+V GIFVSNCT++NTT G+RIKTWA SP
Sbjct: 262 GHGSTNITVSNVTCGPGHGISVGSLGKYDDEKEVRGIFVSNCTIRNTTNGLRIKTWAASP 321

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
             QAT I FQDI+LDSV+NPIIIDQ YGSKK K PS+VKISDV +KNIRGTT+S V VSL
Sbjct: 322 AGQATRITFQDIILDSVRNPIIIDQTYGSKKKKAPSQVKISDVVFKNIRGTTVSDVGVSL 381

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCDGI LE+IDLAF G+  K     NSCLNAK  A GKQNPPAC
Sbjct: 382 QCSAAVPCDGIKLEEIDLAFTGTRLKHPTFANSCLNAKITAIGKQNPPAC 431

BLAST of Carg07158 vs. NCBI nr
Match: KAA0046656.1 (exopolygalacturonase clone [Cucumis melo var. makuwa])

HSP 1 Score: 560.8 bits (1444), Expect = 8.1e-156
Identity = 267/350 (76.29%), Postives = 299/350 (85.43%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVYAGPCD PMTVEIQGTV ATTD+TEYSS
Sbjct: 80  QAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSS 139

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P WILFES+T LNLIG GTFDGQG E W YNDC  +P C +PPTS+KFNKVT+G MEG +
Sbjct: 140 PEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFT 199

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+NSK FHIF+VLSH++++NN++I+AP  SPNTDG+HISQTD V +T+SIIGTGDDCVSI
Sbjct: 200 SVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSI 259

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+ V NI CGPGHGISVGSLGKYKDEK+V GIFVSNCT++NTT GVRIKTWA SP
Sbjct: 260 GHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASP 319

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           P QAT I FQDIVLD V+NPIIIDQ YGSKK + PS+VKISDV +KNIRGTTIS V VSL
Sbjct: 320 PGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSL 379

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCD I LE ID+AF GS R K   GNSCLNAK    GKQNPPAC
Sbjct: 380 QCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNSCLNAKITTIGKQNPPAC 428

BLAST of Carg07158 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 7.3e-99
Identity = 176/356 (49.44%), Postives = 244/356 (68.54%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           +AF+  W++ C S  PA ++ P G FL GP+++AGPC + +TV + GT++ATT  + Y++
Sbjct: 69  KAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVTVNVIGTIIATT--SGYAT 128

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P W LFE +  L L G GTF G+G+  W  + C     C +PPTSLKF  + N  + GIS
Sbjct: 129 PEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQCNLPPTSLKFRNMKNVEINGIS 188

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+N+K FH+FLV + ++ + N+ + AP +SPNTDGIH+S  DNV+I  S I TGDDCVS+
Sbjct: 189 SVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDSTIATGDDCVSV 248

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           G GS NVTV  + CGPGHG+SVGSLGKYK+E+DVSGI V+NCT+  T  G+RIKTW  S 
Sbjct: 249 GRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDNGLRIKTWGGSD 308

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           PS+A  I F++I++ SVKNPIIIDQ YGS+     S+V ISD+ +KNIRGTTI+   V +
Sbjct: 309 PSKAVDIKFENIIMQSVKNPIIIDQNYGSRGG--DSQVAISDILFKNIRGTTITKDVVQI 368

Query: 302 QCSSAIPCDGITLEDIDLAFI---GSDRKKA---LIGNSCLNAKFKATGKQNPPAC 352
            CS ++PC G+ + D++L ++   G ++K +   L+G  C NA     GK + P C
Sbjct: 369 MCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVGALCDNANVIFGGKLSFPMC 420

BLAST of Carg07158 vs. ExPASy Swiss-Prot
Match: W8P8Q3 (Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 PE=1 SV=3)

HSP 1 Score: 317.0 bits (811), Expect = 2.7e-85
Identity = 165/356 (46.35%), Postives = 220/356 (61.80%), Query Frame = 0

Query: 3   AFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEY-SS 62
           A + AW EAC +  PAK+V P G+FL   +   GPC AP+T+EI G   A  DV +    
Sbjct: 42  ALLAAWKEACAAAAPAKIVVPAGEFLLNAVKLQGPCKAPLTIEIAGNFKAPADVAQMKGE 101

Query: 63  PVWILFESITGLN---LIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLME 122
             W+  E++ GL    L   GTFDGQGQ  W  N C  +  C   P + +FN +TN  + 
Sbjct: 102 DTWVKIENVQGLTITCLPTGGTFDGQGQAAWKQNKCAQSGMCNSLPYNFRFNTLTNAQIS 161

Query: 123 GISSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDC 182
           GI SLNSK +H+ ++   ++ +  L I AP  S NTDG+HI +++ V  T+S IGTGDDC
Sbjct: 162 GIKSLNSKLYHMGVMGCKNITLTGLTIDAPKDSLNTDGMHIGRSNGVHATNSKIGTGDDC 221

Query: 183 VSIGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWA 242
           +S+G G+V+V V  I CGPGHGIS+GS+GK+ +E   +GIFV NC+  +T  GVRIK+W 
Sbjct: 222 ISMGDGAVDVHVEGITCGPGHGISIGSMGKFANEAPNTGIFVKNCSFTDTDNGVRIKSWM 281

Query: 243 DSPPSQATGIHFQDIVLDSVKNPIIIDQF---YGSKKNKKPSKVKISDVSYKNIRGTTIS 302
           +S  + A+ +HF+DI + +V NP+IIDQ    Y   K K PSKVK+S +S+KN+ G   S
Sbjct: 282 NSFEASASDLHFEDITVTNVLNPVIIDQEYCPYNHCKEKTPSKVKLSKISFKNVHGAAKS 341

Query: 303 VVPVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
              V L CSSA+PCDG+ L DIDL F G         + C N K   TGKQNP AC
Sbjct: 342 AEVVKLLCSSAVPCDGVELADIDLTFPG-----GAAVSQCKNVKPIVTGKQNPVAC 392

BLAST of Carg07158 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 150/351 (42.74%), Postives = 221/351 (62.96%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPC-DAPMTVEIQGTVLATTDVTEYS 61
           QA MKAW  AC S GP+ V+ P G +  G +   GPC  + +  +I G V A  D +++ 
Sbjct: 25  QAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKFK 84

Query: 62  SPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGI 121
           S  W+ F  I GL + G GT DGQGQ  W  N+C  NP+C     +L+F+ + + ++  I
Sbjct: 85  SDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRDI 144

Query: 122 SSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVS 181
           +SLNSK FHI ++    +   ++ + AP  S NTDGIH+  +  VTIT++ I TGDDC+S
Sbjct: 145 TSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCIS 204

Query: 182 IGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADS 241
           IG GS NVT++ + CGPGHGIS+GSLG+Y +EK+V GI V  CT   T  GVR+KTW +S
Sbjct: 205 IGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNS 264

Query: 242 PPSQATGIHFQDIVLDSVKNPIIIDQF---YGSKKNKKPSKVKISDVSYKNIRGTTISVV 301
           PP  AT + FQD+ +++V+NP+I+DQ    YG    + PS++K+S++++ NIRGT+   V
Sbjct: 265 PPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKV 324

Query: 302 PVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNP 349
            V + CS  +PC  + + +I+L++ G+        ++C N K   +GKQ P
Sbjct: 325 AVVIACSHGMPCSNMKIGEINLSYRGAGGPAT---STCSNVKPTFSGKQVP 372

BLAST of Carg07158 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 4.0e-81
Identity = 154/355 (43.38%), Postives = 216/355 (60.85%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QA   AW EAC S  P+ ++ P G F  G +   GPC + + +++QGT+ A  D ++   
Sbjct: 4   QALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKG 63

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDN-PSCIMPPTSLKFNKVTNGLMEGI 121
             WI    I  L + G G FDGQG+  W+ NDC  N P C     +L+   VTN ++  +
Sbjct: 64  LGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDV 123

Query: 122 SSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVS 181
           ++L+SK FH+ ++   +L      I A + S NTDGIHI ++D V I ++ I TGDDC+S
Sbjct: 124 TTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCIS 183

Query: 182 IGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADS 241
           +G GS N+ ++NI CGPGHGISVGSLG+YK+E+ V GI+V NCT+  +  GVRIKTW  S
Sbjct: 184 LGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKS 243

Query: 242 PPSQATGIHFQDIVLDSVKNPIIIDQF---YGSKKNKKPSKVKISDVSYKNIRGTTISVV 301
            P +A+ +HFQDI ++SV  PI+IDQ    Y     + PS VK+S +S+KNI+GT+ +  
Sbjct: 244 EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKE 303

Query: 302 PVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
            V L CS + PC+G+ L DIDL + G   K     + C N K    GKQ P  C+
Sbjct: 304 AVKLVCSKSFPCNGVELADIDLTYSG---KGGPATSVCENIKPTIKGKQIPAICS 355

BLAST of Carg07158 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 147/353 (41.64%), Postives = 213/353 (60.34%), Query Frame = 0

Query: 3   AFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSSP 62
           A +KA+  AC +  P++V+ P G F  G  V  GPC +P+   +QG V      T+    
Sbjct: 38  AVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQ-GKD 97

Query: 63  VWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGISS 122
            W++FE I G  L G GTFDG+G   W  N+C     C   P S++F+ V N  ++ ++S
Sbjct: 98  RWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTS 157

Query: 123 LNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSIG 182
           L++K FH  ++   ++  +N+ IIAP +SPNTDGIH+ + + V I ++ I TGDDC+S+G
Sbjct: 158 LDAKNFHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVG 217

Query: 183 HGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP- 242
            G  N+ +  + CGPGHGISVGSLG+Y  E+DV+ I V NCTL+ T+ G+RIKTW  +  
Sbjct: 218 DGMKNLLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAAC 277

Query: 243 PSQATGIHFQDIVLDSVKNPIIIDQFY---GSKKNKKPSKVKISDVSYKNIRGTTISVVP 302
            + A GIHF+DI+L+ V NPI+IDQ Y         KPS +K+ D++++NIRGT+ +   
Sbjct: 278 TTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDA 337

Query: 303 VSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           V L CS   PC+ + + DI++ + G D         C N   K  G QNP AC
Sbjct: 338 VKLLCSKGHPCENVEIGDINIEYTGPDGPPTF---ECTNVTPKLVGAQNPKAC 386

BLAST of Carg07158 vs. ExPASy TrEMBL
Match: A0A6J1GQF2 (exopolygalacturonase clone GBGE184 OS=Cucurbita moschata OX=3662 GN=LOC111456159 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.6e-202
Identity = 347/351 (98.86%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTD+TEYSS
Sbjct: 88  QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDITEYSS 147

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS
Sbjct: 148 PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 207

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI
Sbjct: 208 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 267

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP
Sbjct: 268 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 327

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           PSQATGIHFQDIVLDSVK+PIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL
Sbjct: 328 PSQATGIHFQDIVLDSVKSPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 387

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
           QCSSA+PCDGITLEDIDLAFIGSDRKKAL+GNSCLNAKFKATGKQNPPACA
Sbjct: 388 QCSSAVPCDGITLEDIDLAFIGSDRKKALVGNSCLNAKFKATGKQNPPACA 438

BLAST of Carg07158 vs. ExPASy TrEMBL
Match: A0A5D3D1R6 (Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001270 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 267/350 (76.29%), Postives = 299/350 (85.43%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVYAGPCD PMTVEIQGTV ATTD+TEYSS
Sbjct: 80  QAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSS 139

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P WILFES+T LNLIG GTFDGQG E W YNDC  +P C +PPTS+KFNKVT+G MEG +
Sbjct: 140 PEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFT 199

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+NSK FHIF+VLSH++++NN++I+AP  SPNTDG+HISQTD V +T+SIIGTGDDCVSI
Sbjct: 200 SVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSI 259

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+ V NI CGPGHGISVGSLGKYKDEK+V GIFVSNCT++NTT GVRIKTWA SP
Sbjct: 260 GHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASP 319

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           P QAT I FQDIVLD V+NPIIIDQ YGSKK + PS+VKISDV +KNIRGTTIS V VSL
Sbjct: 320 PGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSL 379

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCD I LE ID+AF GS R K   GNSCLNAK    GKQNPPAC
Sbjct: 380 QCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNSCLNAKITTIGKQNPPAC 428

BLAST of Carg07158 vs. ExPASy TrEMBL
Match: A0A5A7TXI9 (Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G00200 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 267/350 (76.29%), Postives = 299/350 (85.43%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVYAGPCD PMTVEIQGTV ATTD+TEYSS
Sbjct: 80  QAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSS 139

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P WILFES+T LNLIG GTFDGQG E W YNDC  +P C +PPTS+KFNKVT+G MEG +
Sbjct: 140 PEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFT 199

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+NSK FHIF+VLSH++++NN++I+AP  SPNTDG+HISQTD V +T+SIIGTGDDCVSI
Sbjct: 200 SVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSI 259

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+ V NI CGPGHGISVGSLGKYKDEK+V GIFVSNCT++NTT GVRIKTWA SP
Sbjct: 260 GHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASP 319

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           P QAT I FQDIVLD V+NPIIIDQ YGSKK + PS+VKISDV +KNIRGTTIS V VSL
Sbjct: 320 PGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSL 379

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCD I LE ID+AF GS R K   GNSCLNAK    GKQNPPAC
Sbjct: 380 QCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNSCLNAKITTIGKQNPPAC 428

BLAST of Carg07158 vs. ExPASy TrEMBL
Match: A0A1S3BRS3 (exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 267/350 (76.29%), Postives = 299/350 (85.43%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVYAGPCD PMTVEIQGTV ATTD+TEYSS
Sbjct: 80  QAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSS 139

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P WILFES+T LNLIG GTFDGQG E W YNDC  +P C +PPTS+KFNKVT+G MEG +
Sbjct: 140 PEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFT 199

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+NSK FHIF+VLSH++++NN++I+AP  SPNTDG+HISQTD V +T+SIIGTGDDCVSI
Sbjct: 200 SVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSI 259

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+ V NI CGPGHGISVGSLGKYKDEK+V GIFVSNCT++NTT GVRIKTWA SP
Sbjct: 260 GHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASP 319

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           P QAT I FQDIVLD V+NPIIIDQ YGSKK + PS+VKISDV +KNIRGTTIS V VSL
Sbjct: 320 PGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSL 379

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCD I LE ID+AF GS R K   GNSCLNAK    GKQNPPAC
Sbjct: 380 QCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNSCLNAKITTIGKQNPPAC 428

BLAST of Carg07158 vs. ExPASy TrEMBL
Match: A0A0A0K9C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.2e-154
Identity = 263/350 (75.14%), Postives = 301/350 (86.00%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QAFM+AWV+ACHS GPAKVVFPPG FLTGPLVYAGPCD PMTVEIQGTV ATTD+++YSS
Sbjct: 84  QAFMQAWVKACHSSGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDISQYSS 143

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
             WILFES+TGLNLIG GTFDGQG +TW YNDC  +P CI+PPTS+KFNKVT GLMEGI+
Sbjct: 144 AEWILFESVTGLNLIGRGTFDGQGADTWKYNDCSKHPQCIIPPTSIKFNKVTQGLMEGIT 203

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+NSK FHIF+VLSH++++NN++I+AP  SPNTDG+HISQTD V +T+SIIGTGDDCVSI
Sbjct: 204 SVNSKAFHIFVVLSHNIKINNVHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSI 263

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           GHGS N+ V NI CGPGHGISVGSLGKY+DEK+V GIFVSNCT++NTT GVRIKTWA SP
Sbjct: 264 GHGSTNINVLNITCGPGHGISVGSLGKYRDEKEVRGIFVSNCTIRNTTNGVRIKTWAASP 323

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           P QAT I FQ+IVLD V+NPIIIDQ YGSK  K PS+VK+SDV +KNIRGTTIS V VSL
Sbjct: 324 PGQATRITFQNIVLDKVRNPIIIDQNYGSKTKKSPSQVKVSDVQFKNIRGTTISPVAVSL 383

Query: 302 QCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
           QCS+A+PCDGI LE ID+AF G+  K+    NSCLNAK    GKQNPPAC
Sbjct: 384 QCSAALPCDGIKLEQIDVAFSGTHLKQP-FANSCLNAKITTIGKQNPPAC 432

BLAST of Carg07158 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 362.1 bits (928), Expect = 5.2e-100
Identity = 176/356 (49.44%), Postives = 244/356 (68.54%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           +AF+  W++ C S  PA ++ P G FL GP+++AGPC + +TV + GT++ATT  + Y++
Sbjct: 69  KAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVTVNVIGTIIATT--SGYAT 128

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
           P W LFE +  L L G GTF G+G+  W  + C     C +PPTSLKF  + N  + GIS
Sbjct: 129 PEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQCNLPPTSLKFRNMKNVEINGIS 188

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+N+K FH+FLV + ++ + N+ + AP +SPNTDGIH+S  DNV+I  S I TGDDCVS+
Sbjct: 189 SVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDSTIATGDDCVSV 248

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           G GS NVTV  + CGPGHG+SVGSLGKYK+E+DVSGI V+NCT+  T  G+RIKTW  S 
Sbjct: 249 GRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDNGLRIKTWGGSD 308

Query: 242 PSQATGIHFQDIVLDSVKNPIIIDQFYGSKKNKKPSKVKISDVSYKNIRGTTISVVPVSL 301
           PS+A  I F++I++ SVKNPIIIDQ YGS+     S+V ISD+ +KNIRGTTI+   V +
Sbjct: 309 PSKAVDIKFENIIMQSVKNPIIIDQNYGSRGG--DSQVAISDILFKNIRGTTITKDVVQI 368

Query: 302 QCSSAIPCDGITLEDIDLAFI---GSDRKKA---LIGNSCLNAKFKATGKQNPPAC 352
            CS ++PC G+ + D++L ++   G ++K +   L+G  C NA     GK + P C
Sbjct: 369 MCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVGALCDNANVIFGGKLSFPMC 420

BLAST of Carg07158 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.7e-85
Identity = 168/364 (46.15%), Postives = 232/364 (63.74%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPA-KVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEY- 61
           +AF+ AW +AC S   +  ++ P G+F  G L ++GPC     + ++  V A+TD+++Y 
Sbjct: 52  KAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSNLTVR--VKASTDLSKYR 111

Query: 62  SSPVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEG 121
           S   WI F  I GL L G GTFDGQG   W +N+C  + +C + PTSLKF  +   ++  
Sbjct: 112 SGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCKLLPTSLKFVGMNRTVVRR 171

Query: 122 ISSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCV 181
           ISS+NSK FHI LV     +   LNI AP  SPNTDGIHI ++ NV  + S I TGDDCV
Sbjct: 172 ISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIERSSNVYFSRSHIATGDDCV 231

Query: 182 SIGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWAD 241
           SIG G+  +T+++IKCGPGHGISVGSLG+Y +EKDV+G+ V +C +  TT G+RIKTWA+
Sbjct: 232 SIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVKDCKISGTTNGIRIKTWAN 291

Query: 242 SPP-SQATGIHFQDIVLDSVKNPIIIDQFY---GSKKNKKPSKVKISDVSYKNIRGTTIS 301
           SP  S AT + F++I++++V NPIIIDQ Y    S  +  PSKV++S++ +KNIRGT+ S
Sbjct: 292 SPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPSKVELSEIYFKNIRGTSSS 351

Query: 302 VVPVSLQCSSAIPCDGITLEDIDLAFIGSD--------RKKALIGNSCLNAKFKATGKQN 352
           +V V L CS  +PC  + LE++ L    SD        R    + +SC N +    G Q 
Sbjct: 352 LVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQSSNRGNEAVSSSCRNVRANYIGTQI 411

BLAST of Carg07158 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.0e-84
Identity = 157/355 (44.23%), Postives = 220/355 (61.97%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QA +KA+  AC S  P+KVV P G+F  G +   GPC AP+ V +QGTV A  +  +   
Sbjct: 37  QALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQ-GK 96

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
             W++F +I G  L G G FDG+G   W  N+C     C   P S++F+ + N  +  IS
Sbjct: 97  EKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDIS 156

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+++K FHI ++ + ++ +NN+ I+AP+ SPNTDGIH+ ++D V I +S I TGDDC+S+
Sbjct: 157 SIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISV 216

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           G G  N+ V  + CGPGHGISVGSLG+Y  E+DVSGI V NCTL+ T  G+RIKTW  + 
Sbjct: 217 GDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAA 276

Query: 242 PS-QATGIHFQDIVLDSVKNPIIIDQFY---GSKKNKKPSKVKISDVSYKNIRGTTISVV 301
            S  A+ IHF+DI+L  V NPI+IDQ Y        +K S +K+ ++S+KNIRGT+ +  
Sbjct: 277 CSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKD 336

Query: 302 PVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
            V L CS   PC  + + DID+ + G+D         C N   K  G Q+P AC+
Sbjct: 337 AVKLLCSKGYPCQNVEIGDIDIKYNGADGPATF---HCSNVSPKILGSQSPKACS 387

BLAST of Carg07158 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.8e-82
Identity = 153/354 (43.22%), Postives = 227/354 (64.12%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QA ++A+  AC S  P KVV P G+F  G ++ +GPC +P+ + + GTVLA  + + +  
Sbjct: 38  QALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGN-SIHGK 97

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
             W++F+ + G  L G GTFDG+G   W  N+C     C   P S++F+ VTN  +  IS
Sbjct: 98  EKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDIS 157

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+++K FHI ++ + ++  +N+ I+AP +SPNTDGIH+ ++  V+I +S I TGDDCVS+
Sbjct: 158 SIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSV 217

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           G G VN+ V N+ CGPGHGISVGSLG+Y  E+DVSGI V NCTL+ T  G+RIKTW  + 
Sbjct: 218 GDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAA 277

Query: 242 PS-QATGIHFQDIVLDSVKNPIIIDQFY---GSKKNKKPSKVKISDVSYKNIRGTTISVV 301
            S  A+ IHF++I+L +V NPI+IDQ Y        +K S +K++++S++ IRGT+ +  
Sbjct: 278 CSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKD 337

Query: 302 PVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPAC 352
            V L CS   PC+ + + DI++ + G+D     +   C N + K  G Q P AC
Sbjct: 338 AVKLLCSKGYPCENVQVGDINIQYTGADGPATFM---CSNVRPKLVGTQFPKAC 387

BLAST of Carg07158 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 3.1e-81
Identity = 153/355 (43.10%), Postives = 222/355 (62.54%), Query Frame = 0

Query: 2   QAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVYAGPCDAPMTVEIQGTVLATTDVTEYSS 61
           QA +KA+  AC +   +KVV   G+F  G +   GPC AP+ + +QGT+ A     +   
Sbjct: 38  QALLKAFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQ-GK 97

Query: 62  PVWILFESITGLNLIGNGTFDGQGQETWMYNDCRDNPSCIMPPTSLKFNKVTNGLMEGIS 121
             W++F  I G  L G G FDG+G   W  N+C     C   P S++F+ V N  +  IS
Sbjct: 98  ERWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDIS 157

Query: 122 SLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNTDGIHISQTDNVTITSSIIGTGDDCVSI 181
           S+++K FHI ++ + ++  +N+ +IAP +SPNTDGIH+ +++ V I +S I TGDDC+S+
Sbjct: 158 SIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISV 217

Query: 182 GHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKDVSGIFVSNCTLKNTTFGVRIKTWADSP 241
           G G  N+ V N+ CGPGHGISVGSLG+Y  E+DV+GI V NCTL+ T  G+RIKTW  + 
Sbjct: 218 GDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAA 277

Query: 242 -PSQATGIHFQDIVLDSVKNPIIIDQFY---GSKKNKKPSKVKISDVSYKNIRGTTISVV 301
             + A+GIHF++I+L++V NPI+IDQ Y        +KPS +K+ D+S+KNIRGT+ +  
Sbjct: 278 CATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKD 337

Query: 302 PVSLQCSSAIPCDGITLEDIDLAFIGSDRKKALIGNSCLNAKFKATGKQNPPACA 353
            V L CS A PC  + + +I+L + G+D     +   C N   K  G QNP AC+
Sbjct: 338 AVKLLCSKAHPCANVEIGNINLEYKGADGPPTFM---CSNVSPKLVGTQNPKACS 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014349.11.0e-203100.00Exopolygalacturonase-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022953710.13.4e-20298.86exopolygalacturonase clone GBGE184 [Cucurbita moschata][more]
XP_023549399.15.2e-19596.01exopolygalacturonase clone GBGE184-like [Cucurbita pepo subsp. pepo][more]
XP_038899827.17.8e-15978.57exopolygalacturonase clone GBGE184 [Benincasa hispida][more]
KAA0046656.18.1e-15676.29exopolygalacturonase clone [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P490627.3e-9949.44Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ... [more]
W8P8Q32.7e-8546.35Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 P... [more]
Q6H9K02.3e-8142.74Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
P245484.0e-8143.38Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
P353371.1e-7841.64Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GQF21.6e-20298.86exopolygalacturonase clone GBGE184 OS=Cucurbita moschata OX=3662 GN=LOC111456159... [more]
A0A5D3D1R63.9e-15676.29Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411... [more]
A0A5A7TXI93.9e-15676.29Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BRS33.9e-15676.29exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 ... [more]
A0A0A0K9C42.2e-15475.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02790.15.2e-10049.44polygalacturonase 4 [more]
AT4G18180.12.7e-8546.15Pectin lyase-like superfamily protein [more]
AT3G07820.13.0e-8444.23Pectin lyase-like superfamily protein [more]
AT3G07830.12.8e-8243.22Pectin lyase-like superfamily protein [more]
AT3G07840.13.1e-8143.10Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 135..161
e-value: 610.0
score: 8.8
coord: 185..205
e-value: 2100.0
score: 5.0
coord: 162..183
e-value: 590.0
score: 8.9
coord: 276..298
e-value: 9000.0
score: 0.4
coord: 215..236
e-value: 9.2
score: 15.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 12..324
e-value: 8.1E-91
score: 304.4
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 192..205
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..350
e-value: 1.3E-104
score: 352.4
NoneNo IPR availablePANTHERPTHR31375:SF158POLYGALACTURONASEcoord: 2..348
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..348
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 2..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07158-RACarg07158-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity