Carg06666 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06666
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tonoplast intrinsic protein 2
Location	Carg_Chr18: 544821 .. 546369 (+)
RNA-Seq Expression	Carg06666
Synteny	Carg06666

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTGGACGGAAAGGAGATGGGCCTCAGCGATATCGAAACAAGGTCTGTCAGTATCTTCGTCCAAAACAGAACACCTCCAAAAACTCTAAAACAAATCCAAAGCTATGGAGACAAGACACTGCTTGGAGTCGAAATTGAAGAGCTTACACATATCATAACGTGTCGCCCCAAAGCCACGTCATCTCAACACGTGTCCCACCCTCTTTCTCAAGCCCCATTTTCCCCATTTTAAATCCCTCGGTCCTCCGCTTCTCAGCCACCGTTAAAAGCATCAAATTTCAAACGCTTCTGGTTGGATCCTCTGAGCAAAAGGAAGTGTTGGTGGTTCGTTTTCAGCTGTTTTTGCCGCCGCCGCCGCCATGCCGCCGAGACGATATGCCGTTGGACGGGCCGATGAGGCCACCAACCCCGACTCCGTTAGAGCCACCTTGGCTGAGTTCCTTTCCACTTTCATCTTCGTCTTTGCCGGAGAAGGCTCCGTCCTCGCTCTCGGTACGATACCTTTTAAATTCCAAGTACATACATGCATGCTTTCATGACCCATCAGATCTGTTAAGAATCACGACCTTCTACAGTGCTATAATATTGTCCACTTTGATCCTAAGCTCTCATGGCTTTGTTTTGGTCTCATATCAATGAAGACAGTATTCTTGACTTATAAACTCATAATCATTGTCTAAATTCACTCCCAACCATTCTCAACAGATGGATCATTAGTTCTTTGTTTTGGTTTGCAGATAAAATTTTCAAGCCAGCAGACTATGGAAGCTACGGTCGTGGTGGTTATGGTTACAGCCATGGTCATGGTTACGGTCGAAGGGGAGGCGACACGGGCCGTGGGGCATCGGATTTAGTAGTAATAGCAATAGCGCATGCATTTGCGCTGTTCTCGGCCGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTTACATTTGGTGTACTTCTAGGAGGAAGGATATCTCTTATCCGTGCATTCTTCTATTGGGTAGCTCAGATTTTGGGCGCCATTGTGGCTTCCCTGATCTTGAGACTCGCAACGGGTGGCATGGTAAAAACACACAGGCACATGCACACATTCTTTTTTACCATAATGTGAATTTTAGCTATAATTTACTGTTTTACTTTGTGGGTAAATGGCAGAGGCCAATGGGGTTCTTCGTTTCATCGGGCATATCAGAACTGCACGGGTTTCTACTAGAGATATTGCTTACATTTGCTTTGGTGTACGTAGTCTACGCAACAGCAGTAGACCCGAAGAGGGGAAGTTTGGAAACAATAGCACCACTGGCAATCGGGTTAATAGTCGGGGCGAATATTCTAGTGGGTGGGGTATTTGATGGGGCCTGTATGAACCCGGCCCGGGCCTTTGGGCCGTCGTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCACTTGTCTATGAGTATTTGGTGATTCCAGTTGAACCTCCTGTACATGCCCCCCACCAGCCTTTAGCTCCGGAAGATTACTGA

mRNA sequence

ATGGCTTGGACGGAAAGGAGATGGGCCTCAGCGATATCGAAACAAGCTGTTTTTGCCGCCGCCGCCGCCATGCCGCCGAGACGATATGCCGTTGGACGGGCCGATGAGGCCACCAACCCCGACTCCGTTAGAGCCACCTTGGCTGAGTTCCTTTCCACTTTCATCTTCGTCTTTGCCGGAGAAGGCTCCGTCCTCGCTCTCGATAAAATTTTCAAGCCAGCAGACTATGGAAGCTACGGTCGTGGTGGTTATGGTTACAGCCATGGTCATGGTTACGGTCGAAGGGGAGGCGACACGGGCCGTGGGGCATCGGATTTAGTAGTAATAGCAATAGCGCATGCATTTGCGCTGTTCTCGGCCGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTTACATTTGGTGTACTTCTAGGAGGAAGGATATCTCTTATCCGTGCATTCTTCTATTGGGTAGCTCAGATTTTGGGCGCCATTGTGGCTTCCCTGATCTTGAGACTCGCAACGGGTGGCATGAGGCCAATGGGGTTCTTCGTTTCATCGGGCATATCAGAACTGCACGGGTTTCTACTAGAGATATTGCTTACATTTGCTTTGGTGTACGTAGTCTACGCAACAGCAGTAGACCCGAAGAGGGGAAGTTTGGAAACAATAGCACCACTGGCAATCGGGTTAATAGTCGGGGCGAATATTCTAGTGGGTGGGGTATTTGATGGGGCCTGTATGAACCCGGCCCGGGCCTTTGGGCCGTCGTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCACTTGTCTATGAGTATTTGGTGATTCCAGTTGAACCTCCTGTACATGCCCCCCACCAGCCTTTAGCTCCGGAAGATTACTGA

Coding sequence (CDS)

ATGGCTTGGACGGAAAGGAGATGGGCCTCAGCGATATCGAAACAAGCTGTTTTTGCCGCCGCCGCCGCCATGCCGCCGAGACGATATGCCGTTGGACGGGCCGATGAGGCCACCAACCCCGACTCCGTTAGAGCCACCTTGGCTGAGTTCCTTTCCACTTTCATCTTCGTCTTTGCCGGAGAAGGCTCCGTCCTCGCTCTCGATAAAATTTTCAAGCCAGCAGACTATGGAAGCTACGGTCGTGGTGGTTATGGTTACAGCCATGGTCATGGTTACGGTCGAAGGGGAGGCGACACGGGCCGTGGGGCATCGGATTTAGTAGTAATAGCAATAGCGCATGCATTTGCGCTGTTCTCGGCCGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTTACATTTGGTGTACTTCTAGGAGGAAGGATATCTCTTATCCGTGCATTCTTCTATTGGGTAGCTCAGATTTTGGGCGCCATTGTGGCTTCCCTGATCTTGAGACTCGCAACGGGTGGCATGAGGCCAATGGGGTTCTTCGTTTCATCGGGCATATCAGAACTGCACGGGTTTCTACTAGAGATATTGCTTACATTTGCTTTGGTGTACGTAGTCTACGCAACAGCAGTAGACCCGAAGAGGGGAAGTTTGGAAACAATAGCACCACTGGCAATCGGGTTAATAGTCGGGGCGAATATTCTAGTGGGTGGGGTATTTGATGGGGCCTGTATGAACCCGGCCCGGGCCTTTGGGCCGTCGTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCACTTGTCTATGAGTATTTGGTGATTCCAGTTGAACCTCCTGTACATGCCCCCCACCAGCCTTTAGCTCCGGAAGATTACTGA

Protein sequence

MAWTERRWASAISKQAVFAAAAAMPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGGYGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY

Homology

[Close]

BLAST of Carg06666 vs. NCBI nr
Match: KAG6573002.1 (putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012187.1 putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.4 bits (1539), Expect = 6.8e-167
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 1   MAWTERRWASAISKQAVFAAAAAMPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAG 60
           MAWTERRWASAISKQAVFAAAAAMPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAG
Sbjct: 1   MAWTERRWASAISKQAVFAAAAAMPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAG 60

Query: 61  EGSVLALDKIFKPADYGSYGRGGYGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSA 120
           EGSVLALDKIFKPADYGSYGRGGYGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSA
Sbjct: 61  EGSVLALDKIFKPADYGSYGRGGYGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSA 120

Query: 121 VAASINVSGGHVNPAVTFGVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMG 180
           VAASINVSGGHVNPAVTFGVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMG
Sbjct: 121 VAASINVSGGHVNPAVTFGVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMG 180

Query: 181 FFVSSGISELHGFLLEILLTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGV 240
           FFVSSGISELHGFLLEILLTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGV
Sbjct: 181 FFVSSGISELHGFLLEILLTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGV 240

Query: 241 FDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPL 300
           FDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPL
Sbjct: 241 FDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPL 300

Query: 301 APEDY 306
           APEDY
Sbjct: 301 APEDY 305

[Close]

BLAST of Carg06666 vs. NCBI nr
Match: XP_022955131.1 (probable aquaporin TIP3-2 [Cucurbita moschata])

HSP 1 Score: 555.8 bits (1431), Expect = 2.3e-154
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG
Sbjct: 1   MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
           YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 282

[Close]

BLAST of Carg06666 vs. NCBI nr
Match: XP_023542498.1 (probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 544.7 bits (1402), Expect = 5.2e-151
Identity = 276/282 (97.87%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEAT+PDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG
Sbjct: 1   MPPRRYAVGRADEATHPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
           YGYSH HGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  YGYSHDHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGF+LEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFVLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATA+DPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAIDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVH  HQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHTHHQPLAPEDY 282

[Close]

BLAST of Carg06666 vs. NCBI nr
Match: XP_022994656.1 (probable aquaporin TIP3-2 [Cucurbita maxima] >pir|T10253| membrane protein MP28 - cucurbit >BAA08108.1 MP28 [Cucurbita hybrid cultivar])

HSP 1 Score: 514.6 bits (1324), Expect = 5.8e-142
Identity = 267/282 (94.68%), Postives = 268/282 (95.04%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEAT+PDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS     
Sbjct: 1   MPPRRYAVGRADEATHPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS----- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                   YGRRGGDTGR ASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  --------YGRRGGDTGRTASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 269

[Close]

BLAST of Carg06666 vs. NCBI nr
Match: XP_038895592.1 (probable aquaporin TIP3-2 [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 3.7e-141
Identity = 257/285 (90.18%), Postives = 270/285 (94.74%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYA GRADEAT+PDS+RATLAEF+STFIFVFAGEGSVLALDKIF+PADYGS+G G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSHGHGS 60

Query: 84  Y---GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGV 143
           Y   GY +GHGYGRRG DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFG 
Sbjct: 61  YGRGGYGYGHGYGRRGADTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120

Query: 144 LLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLT 203
           LLGGRISLIRAFFYWVAQILGAI+ASL+LRLATGGMRPMGFFVSSGISELHGFLLEI+LT
Sbjct: 121 LLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLT 180

Query: 204 FALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWR 263
           FALVY VYATA+DPKRGSL T+APLAIGLIVGANILVGG FDGACMNPARAFGPSLVGWR
Sbjct: 181 FALVYTVYATAIDPKRGSLGTMAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240

Query: 264 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPP+H  HQPLAPEDY
Sbjct: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTGHQPLAPEDY 285

[Close]

BLAST of Carg06666 vs. ExPASy Swiss-Prot
Match: O22588 (Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 2.9e-104
Identity = 193/283 (68.20%), Postives = 229/283 (80.92%), Query Frame = 0


Query: 27  RRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIF--KPADYGSYGRGGY 86
           R Y  GRADEAT+PDS+RATLAEFLSTF+FVFAGEGS+LALDK++    A  G+   GG 
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKLYWDTAAHTGTNTPGG- 66

Query: 87  GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGG 146
                                LV++A+AHA ALF+AV+A+INVSGGHVNPAVTF  L+GG
Sbjct: 67  ---------------------LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGG 126

Query: 147 RISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALV 206
           RIS+IRA +YWVAQ++GAI+A L+LRLAT G+RP+GF V+SG+SELHG L+EI+LTFALV
Sbjct: 127 RISVIRAIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALV 186

Query: 207 YVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNH 266
           YVVY+TA+DPKRGS+  IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NH
Sbjct: 187 YVVYSTAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNH 246

Query: 267 WIYWIGPLLGGGLAALVYEYLVIPV--EPPVHAPHQPLAPEDY 306
           WIYW+GP +GG LAAL+YEY++IP   EPP H+ HQPLAPEDY
Sbjct: 247 WIYWVGPFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

[Close]

BLAST of Carg06666 vs. ExPASy Swiss-Prot
Match: P26587 (Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 5.3e-98
Identity = 182/282 (64.54%), Postives = 222/282 (78.72%), Query Frame = 0


Query: 27  RRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGGYGY 86
           R Y  GRADEAT+PDS+RATLAEFLSTF+FVFA EGS+L+LDK++              +
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLY--------------W 66

Query: 87  SHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGGRI 146
            H       G +T  G   L+++A+AHAFALF+AV+A+INVSGGHVNPAVTFG L+GGR+
Sbjct: 67  EHA---AHAGTNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRV 126

Query: 147 SLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALVYV 206
           + IRA +YW+AQ+LGAI+A L+LRL T GMRP+GF ++SG+  ++G +LEI+LTF LVYV
Sbjct: 127 TAIRAIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYV 186

Query: 207 VYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNHWI 266
           VY+T +DPKRGSL  IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWI
Sbjct: 187 VYSTLIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWI 246

Query: 267 YWIGPLLGGGLAALVYEYLVIPVEPP---VHAPHQPLAPEDY 306
           YW+GP +G  LAAL+YEY+VIP EPP    H  HQPLAPEDY
Sbjct: 247 YWVGPFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

[Close]

BLAST of Carg06666 vs. ExPASy Swiss-Prot
Match: P23958 (Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.2e-96
Identity = 180/282 (63.83%), Postives = 217/282 (76.95%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           M  RRY+ GR DEAT+PDS+RA+LAEF STFIFVFAGEGS LAL KI++           
Sbjct: 1   MATRRYSFGRTDEATHPDSMRASLAEFASTFIFVFAGEGSGLALVKIYQ----------- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                         D+   A +L+ +A+AHAFALF+AV+AS++VSGGHVNPAV+FG L+G
Sbjct: 61  --------------DSAFSAGELLALALAHAFALFAAVSASMHVSGGHVNPAVSFGALIG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRIS+IRA +YW+AQ+LG+IVA+L+LRL T  MRP GF VS G+   H F+LE+++TF L
Sbjct: 121 GRISVIRAVYYWIAQLLGSIVAALVLRLVTNNMRPSGFHVSPGVGVGHMFILEVVMTFGL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           +Y VY TA+DPKRG++  IAPLAIGLIVGANILVGG FDGACMNPA AFGPSLVGW+W  
Sbjct: 181 MYTVYGTAIDPKRGAVSYIAPLAIGLIVGANILVGGPFDGACMNPALAFGPSLVGWQWHQ 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWI+W+GPLLG  LAALVYEY VIP+EPP H  HQPLA EDY
Sbjct: 241 HWIFWVGPLLGAALAALVYEYAVIPIEPPPH-HHQPLATEDY 256

[Close]

BLAST of Carg06666 vs. ExPASy Swiss-Prot
Match: Q9ATL7 (Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.5e-88
Identity = 163/282 (57.80%), Postives = 207/282 (73.40%), Query Frame = 0


Query: 25  PPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGGY 84
           P RR+ VGR+++AT+PD++RA ++EF++T IFVFA EGSVL+L K++             
Sbjct: 7   PGRRFTVGRSEDATHPDTIRAAISEFIATAIFVFAAEGSVLSLGKMY------------- 66

Query: 85  GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGG 144
                H     GG        LV +A+AHA AL  AVA ++N+SGGHVNPAVTFG L+GG
Sbjct: 67  -----HDMSTAGG--------LVAVALAHALALAVAVAVAVNISGGHVNPAVTFGALVGG 126

Query: 145 RISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALV 204
           R+SL+RA  YWVAQ+LGA+ A+L+LRLATGGMRP GF ++SG+ + H  LLE ++TF L+
Sbjct: 127 RVSLVRAVLYWVAQLLGAVAATLLLRLATGGMRPPGFALASGVGDWHAVLLEAVMTFGLM 186

Query: 205 YVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNH 264
           Y  YAT +DPKRG + TIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVGWRW +H
Sbjct: 187 YAYYATVIDPKRGHVGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVFGPALVGWRWRHH 246

Query: 265 WIYWIGPLLGGGLAALVYEYLVIP-VEPPVHAPHQPLAPEDY 306
           W+YW+GP LG GLA LVYEYLVIP  +  V   HQPLAPEDY
Sbjct: 247 WVYWLGPFLGAGLAGLVYEYLVIPSADAAVPHAHQPLAPEDY 262

[Close]

BLAST of Carg06666 vs. ExPASy Swiss-Prot
Match: Q9FWV6 (Probable aquaporin TIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.1e-87
Identity = 163/289 (56.40%), Postives = 211/289 (73.01%), Query Frame = 0


Query: 19  AAAAAMPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS 78
           + AAA P RR+ VGR+++AT+PD++RA ++EFL+T IFVFA EGS+L+L K+++  D  +
Sbjct: 2   STAAARPGRRFTVGRSEDATHPDTIRAAISEFLATAIFVFAAEGSILSLGKLYQ--DMST 61

Query: 79  YGRGGYGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTF 138
            G                         LV +++AHA AL  AVA ++N+SGGHVNPA+TF
Sbjct: 62  PG------------------------GLVAVSLAHALALAVAVAVAVNISGGHVNPAITF 121

Query: 139 GVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEIL 198
           G LLGGR+SLIRA FYW+AQ+LGA+VA+L+LRL TGGMRP GF ++SG+ + H  LLE  
Sbjct: 122 GALLGGRLSLIRALFYWLAQLLGAVVATLLLRLTTGGMRPPGFALASGVGDWHAVLLEAT 181

Query: 199 LTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVG 258
           +TF L+Y  YAT +DPKRG + TIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVG
Sbjct: 182 MTFGLMYAYYATVIDPKRGHVGTIAPLAVGFLLGANMLAGGPFDGAGMNPARVFGPALVG 241

Query: 259 WRWDNHWIYWIGPLLGGGLAALVYEYLVIPV--EPPVHAPHQPLAPEDY 306
           WRW +HW+YW+GP +G GLA L+YEYLVIP     P    HQPLAPEDY
Sbjct: 242 WRWRHHWVYWLGPFVGAGLAGLLYEYLVIPSADAAPHGGAHQPLAPEDY 264

[Close]

BLAST of Carg06666 vs. ExPASy TrEMBL
Match: A0A6J1GSW1 (probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111457190 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG
Sbjct: 1   MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
           YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 282

[Close]

BLAST of Carg06666 vs. ExPASy TrEMBL
Match: A0A6J1JZS6 (probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111490303 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.8e-142
Identity = 267/282 (94.68%), Postives = 268/282 (95.04%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEAT+PDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS     
Sbjct: 1   MPPRRYAVGRADEATHPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS----- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                   YGRRGGDTGR ASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  --------YGRRGGDTGRTASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 269

[Close]

BLAST of Carg06666 vs. ExPASy TrEMBL
Match: Q39647 (MP28 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.8e-142
Identity = 267/282 (94.68%), Postives = 268/282 (95.04%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYAVGRADEAT+PDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS     
Sbjct: 1   MPPRRYAVGRADEATHPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGS----- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                   YGRRGGDTGR ASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG
Sbjct: 61  --------YGRRGGDTGRTASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL
Sbjct: 121 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN
Sbjct: 181 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY
Sbjct: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 269

[Close]

BLAST of Carg06666 vs. ExPASy TrEMBL
Match: A0A0A0LTX0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.4e-140
Identity = 256/290 (88.28%), Postives = 271/290 (93.45%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYA GRADEAT+PDS+RATLAEF+STFIFVFAGEGSVLALDKIF+PADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 84  Y--------GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPA 143
           Y        GYS+GHGYGR+G DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPA
Sbjct: 61  YGHGSYGRGGYSYGHGYGRKGTDTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPA 120

Query: 144 VTFGVLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLL 203
           VTFG L+GGRISLIRAFFYWVAQILGAI+ASL+LRLATGGMRPMGFFVSSG+SELHGFLL
Sbjct: 121 VTFGALIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLL 180

Query: 204 EILLTFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPS 263
           EI+LTFALVY VYATA+DPKRGSL TIAPLAIGLIVGANILVGG FDGACMNPARAFGPS
Sbjct: 181 EIILTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240

Query: 264 LVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           LVGWRWDNHWIYWIGPL+GGGLAALVYEYLVIPVEPP+H  HQPLAPEDY
Sbjct: 241 LVGWRWDNHWIYWIGPLIGGGLAALVYEYLVIPVEPPLHT-HQPLAPEDY 289

[Close]

BLAST of Carg06666 vs. ExPASy TrEMBL
Match: A0A1S3AU56 (probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.4e-140
Identity = 255/285 (89.47%), Postives = 270/285 (94.74%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MPPRRYA GRADEAT+PDS+RATLAEF+STFIFVFAGEGSVLALDKIF+PADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 84  Y---GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGV 143
           Y   G+ +GHGYGR+G DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFG 
Sbjct: 61  YGRGGHGYGHGYGRKGADTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120

Query: 144 LLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLT 203
           L+GGRISLIRAFFYWVAQILGAI+ASL+LRLATGGMRPMGFFVSSG+SELHGFLLEI+LT
Sbjct: 121 LIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLLEIILT 180

Query: 204 FALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWR 263
           FALVY VYATA+DPKRGSL TIAPLAIGLIVGANILVGG FDGACMNPARAFGPSLVGWR
Sbjct: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240

Query: 264 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPP+H  HQPLAPEDY
Sbjct: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHT-HQPLAPEDY 284

[Close]

BLAST of Carg06666 vs. TAIR 10
Match: AT1G17810.1 (beta-tonoplast intrinsic protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.1e-105
Identity = 193/283 (68.20%), Postives = 229/283 (80.92%), Query Frame = 0


Query: 27  RRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIF--KPADYGSYGRGGY 86
           R Y  GRADEAT+PDS+RATLAEFLSTF+FVFAGEGS+LALDK++    A  G+   GG 
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKLYWDTAAHTGTNTPGG- 66

Query: 87  GYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGG 146
                                LV++A+AHA ALF+AV+A+INVSGGHVNPAVTF  L+GG
Sbjct: 67  ---------------------LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGG 126

Query: 147 RISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALV 206
           RIS+IRA +YWVAQ++GAI+A L+LRLAT G+RP+GF V+SG+SELHG L+EI+LTFALV
Sbjct: 127 RISVIRAIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALV 186

Query: 207 YVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNH 266
           YVVY+TA+DPKRGS+  IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NH
Sbjct: 187 YVVYSTAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNH 246

Query: 267 WIYWIGPLLGGGLAALVYEYLVIPV--EPPVHAPHQPLAPEDY 306
           WIYW+GP +GG LAAL+YEY++IP   EPP H+ HQPLAPEDY
Sbjct: 247 WIYWVGPFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

[Close]

BLAST of Carg06666 vs. TAIR 10
Match: AT1G73190.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 3.8e-99
Identity = 182/282 (64.54%), Postives = 222/282 (78.72%), Query Frame = 0


Query: 27  RRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGGYGY 86
           R Y  GRADEAT+PDS+RATLAEFLSTF+FVFA EGS+L+LDK++              +
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLY--------------W 66

Query: 87  SHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLGGRI 146
            H       G +T  G   L+++A+AHAFALF+AV+A+INVSGGHVNPAVTFG L+GGR+
Sbjct: 67  EHA---AHAGTNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRV 126

Query: 147 SLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFALVYV 206
           + IRA +YW+AQ+LGAI+A L+LRL T GMRP+GF ++SG+  ++G +LEI+LTF LVYV
Sbjct: 127 TAIRAIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYV 186

Query: 207 VYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDNHWI 266
           VY+T +DPKRGSL  IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWI
Sbjct: 187 VYSTLIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWI 246

Query: 267 YWIGPLLGGGLAALVYEYLVIPVEPP---VHAPHQPLAPEDY 306
           YW+GP +G  LAAL+YEY+VIP EPP    H  HQPLAPEDY
Sbjct: 247 YWVGPFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

[Close]

BLAST of Carg06666 vs. TAIR 10
Match: AT2G36830.1 (gamma tonoplast intrinsic protein )

HSP 1 Score: 285.0 bits (728), Expect = 6.9e-77
Identity = 148/282 (52.48%), Postives = 186/282 (65.96%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MP R  A+GR DEAT PD+++A LAEF+ST IFV AG GS +A +K+ +           
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTE----------- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                       G  T  G   LV  A+AHAF LF AV+   N+SGGHVNPAVTFG  +G
Sbjct: 61  -----------NGATTPSG---LVAAAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           G I+L+R   YW+AQ+LG++VA LIL+ ATGG+    F +S+G+  L+ F+ EI++TF L
Sbjct: 121 GNITLLRGILYWIAQLLGSVVACLILKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VY VYATA+DPK GSL TIAP+AIG IVGANIL GG F GA MNPA AFGP++V W W N
Sbjct: 181 VYTVYATAIDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HW+YW GPL+GGG+A L+YE   I      +  H+ L   DY
Sbjct: 241 HWVYWAGPLVGGGIAGLIYEVFFI------NTTHEQLPTTDY 251

[Close]

BLAST of Carg06666 vs. TAIR 10
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3 )

HSP 1 Score: 273.1 bits (697), Expect = 2.7e-73
Identity = 142/282 (50.35%), Postives = 184/282 (65.25%), Query Frame = 0


Query: 24  MPPRRYAVGRADEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRGG 83
           MP  R A+G   EA+ PD++RA  AEF S  IFVFAG+GS                    
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGS-------------------- 60

Query: 84  YGYSHGHGYGRRGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGVLLG 143
                G  YG+  GD     + LV  +++HAFALF AV+   NVSGGHVNPAVTFG  +G
Sbjct: 61  -----GMAYGKLTGDGPATPAGLVAASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIG 120

Query: 144 GRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILLTFAL 203
           G I+L+RA  YW+AQ+LGA+VA L+L+++TGGM    F +S G++  +  + EI++TF L
Sbjct: 121 GNITLLRAILYWIAQLLGAVVACLLLKVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGL 180

Query: 204 VYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGWRWDN 263
           VY VYATAVDPK+G +  IAPLAIGLIVGANILVGG FDGA MNPA +FGP++V W W N
Sbjct: 181 VYTVYATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTN 240

Query: 264 HWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
           HW+YW+GP +G  +AA+VY+ + I         H+PL   D+
Sbjct: 241 HWVYWVGPFIGAAIAAIVYDTIFIGSN-----GHEPLPSNDF 252

[Close]

BLAST of Carg06666 vs. TAIR 10
Match: AT3G26520.1 (tonoplast intrinsic protein 2 )

HSP 1 Score: 271.6 bits (693), Expect = 7.9e-73
Identity = 146/286 (51.05%), Postives = 183/286 (63.99%), Query Frame = 0


Query: 24  MPPRRYAVGRA-DEATNPDSVRATLAEFLSTFIFVFAGEGSVLALDKIFKPADYGSYGRG 83
           MP R  A+G   +E  +P+++RA LAEF+ST IFVFAG GS +A +KI            
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKI------------ 60

Query: 84  GYGYSHGHGYGRRGGDTGRGA---SDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFG 143
                           T  GA   S LV  A+AHAF LF AV+   N+SGGHVNPAVTFG
Sbjct: 61  ----------------TDNGATTPSGLVAAALAHAFGLFVAVSVGANISGGHVNPAVTFG 120

Query: 144 VLLGGRISLIRAFFYWVAQILGAIVASLILRLATGGMRPMGFFVSSGISELHGFLLEILL 203
           VLLGG I+L+R   YW+AQ+LG++ A  +L  ATGG     F +S+G+  L+  + EI++
Sbjct: 121 VLLGGNITLLRGILYWIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVGSLNALVFEIVM 180

Query: 204 TFALVYVVYATAVDPKRGSLETIAPLAIGLIVGANILVGGVFDGACMNPARAFGPSLVGW 263
           TF LVY VYATAVDPK GSL TIAP+AIG IVGANIL GG F GA MNPA AFGP++V W
Sbjct: 181 TFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSW 240

Query: 264 RWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPVHAPHQPLAPEDY 306
            W NHW+YW GPL+GGGLA ++Y+++ I         H+ L   DY
Sbjct: 241 TWTNHWVYWAGPLIGGGLAGIIYDFVFID-----ENAHEQLPTTDY 253

The following BLAST results are available for this feature:

BLAST of Carg06666 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573002.1	6.8e-167	100.00	putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7...	[more]
XP_022955131.1	2.3e-154	100.00	probable aquaporin TIP3-2 [Cucurbita moschata]	[more]
XP_023542498.1	5.2e-151	97.87	probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo]	[more]
XP_022994656.1	5.8e-142	94.68	probable aquaporin TIP3-2 [Cucurbita maxima] >pir\|T10253\| membrane protein MP28 ...	[more]
XP_038895592.1	3.7e-141	90.18	probable aquaporin TIP3-2 [Benincasa hispida]	[more]

BLAST of Carg06666 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22588	2.9e-104	68.20	Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1	[more]
P26587	5.3e-98	64.54	Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1	[more]
P23958	2.2e-96	63.83	Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1	[more]
Q9ATL7	6.5e-88	57.80	Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1	[more]
Q9FWV6	1.1e-87	56.40	Probable aquaporin TIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=TIP3-1 PE=...	[more]

BLAST of Carg06666 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSW1	1.1e-154	100.00	probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111457190 PE=3 SV=...	[more]
A0A6J1JZS6	2.8e-142	94.68	probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111490303 PE=3 SV=1	[more]
Q39647	2.8e-142	94.68	MP28 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1	[more]
A0A0A0LTX0	3.4e-140	88.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1	[more]
A0A1S3AU56	3.4e-140	89.47	probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1	[more]

BLAST of Carg06666 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17810.1	2.1e-105	68.20	beta-tonoplast intrinsic protein	[more]
AT1G73190.1	3.8e-99	64.54	Aquaporin-like superfamily protein	[more]
AT2G36830.1	6.9e-77	52.48	gamma tonoplast intrinsic protein	[more]
AT4G01470.1	2.7e-73	50.35	tonoplast intrinsic protein 1;3	[more]
AT3G26520.1	7.9e-73	51.05	tonoplast intrinsic protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 150..169 score: 42.27 coord: 265..285 score: 44.9 coord: 113..137 score: 45.71 coord: 195..213 score: 38.88 coord: 228..250 score: 42.23 coord: 44..63 score: 44.65
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 38..282 e-value: 1.6E-72 score: 243.9
IPR000425	Major intrinsic protein	TIGRFAM	TIGR00861	TIGR00861	coord: 48..282 e-value: 3.5E-57 score: 191.7
IPR000425	Major intrinsic protein	CDD	cd00333	MIP	coord: 44..285 e-value: 2.77842E-66 score: 205.565
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 28..291 e-value: 1.4E-76 score: 258.9
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 37..293
IPR034294	Aquaporin transporter	PANTHER	PTHR45665	AQUAPORIN-8	coord: 97..305 coord: 25..72
None	No IPR available	PANTHER	PTHR45665:SF39	SUBFAMILY NOT NAMED	coord: 97..305 coord: 25..72
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 131..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06666-RA	Carg06666-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0006833	water transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015250	water channel activity
molecular_function	GO:0015267	channel activity