Carg06395 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06395
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr14: 9485745 .. 9487924 (-)
RNA-Seq Expression	Carg06395
Synteny	Carg06395

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTTCCTTTTCAGTTTCCAAATTCCAAACCCTAAACCTCCTTCTCTCTTCCTCTCTTTTCCATCCATTCCTCCATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACACCAAGCTTTCCGTCAGAAAACTCGCCGCCGACTCATTATACTACTTCTCTCTATTGTTCTTCTTATTGCTTTGGTCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGGAACTCTTCCTCTTCTTCGACCACGAGTTCGACTCCTCCCACTGAGCTCCCTCCGGCCGCTTCACTCAAAACTCTCTGTAGCGTCACTCAGTATCCGAGTTCCTGTCAATCCTCTCTCGAAAACTCCAACACCACCGATCCGATATTTCTCTTCAAGCTTTCTCTACGAGTCGCCACCGATTCGATCTCAAAGCTCTCTGATTATGCCTCTAACCTAAATTCCAGTACTAGTGATGTGAAGGTTAAAGCTGCGATTGGAATCTGCTCGTCTGTTTTTAAAGATGCCATTGACACACTAAACGACACCGTTTCATCCATGGAAGTGGATCGCCATGGCGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACAATCACAGACCAAGAAACCTGCCTCGATGCCATTCGAGATCTAAACCAGACCGCGGTTCTCGAAAATTTGCAAGCGGTAATGGCGAACTCGACCGAGTTCACTAGCAATAGTTTAGCGATTGTGACCAAAATCCTGGGGCTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGCGTTCCCGGAGTGGTTAAGCCCCGGCGATCGGAGGCTTTTGCAGGAGAACAATGTGACGGTGCATGCGACGGTGTCGAAGGATGGGACAGGGGATTTCACGACGATCAAAGAGGCCGTAGCAGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATCTATGTAAAAGAAGGATTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGCAAAACCAGAACCATTGTTTCAGGCCGCCTTAACTTCATCGACGGCACTCCCACCTACGCCACCGCCACTTTTGGTAATCCATTGAATGATTTTCATTGTTCTTAATTGCTTCATTTAATCTATCAGCAATTTGATGTGTTCATAGACTTAAATTAATTTTAGTTTAAAATAGGAACCTAAAAGTTTTTTCCGATAATCAGCGTATTGATTGGGTACATAGGCCACTACCCTTGTCGTCAGAAACCCACATGGTCGAGTTGTCAGCCATGTGAGGGCAACTTTCCACCACTTTCCACCACTTCCCCTTTTGACCTGTCGTTTCTTCTTCCCTGTTCTGTGAATTGGCTGTTGTGTTTGATTAATTTTGTTTGGTTCTGCAGCTGTTCAAGGCAAAGGATTCATCGGGAAGGATATGGGGTTCATCAACACAGCCGGGCCGGCCAAACACCAGGCCGTCGCCTTCCGGTCAGGCTCCGACCTATCTGTGTTGTACGGATGTTCATTTGATGGCTATCAAGACACTCTTTACGCCCATTCAAACCGTCAATTCTACAGGAACTGCGACATCACCGGCACAATCGACTTCATCTTCGGCAACGCCGCCGTGGTATTCCAGAACTGCAACATCCGGCCCAGGCAGCCACTTCCCAATCAGTTCAACACCATCACAGCCCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCTGCCTACAACGACAGTCTCACTGCCCCCACTTACTTGGGGCGGCCATGGAAGGAATTTTCCACCACCGTGATTATGCAGTCGGAGATTGGGGGCTTCCTGCAGCCCATCGGGTGGAAGGAATGGGTGAGCGGGCAGGACCCACCCAGCAGCATATTTTACGGAGAGTATCAGAATAGTGGGCCTGGATCCAACGTGGACAAGAGGGTCAAATGGACCGGTTACAGGCCCAGTCTTACCGACGCCGAGGCCGCAAAGTTCACCGTCCGGACGTTCCTCAACGGCGAGGAATGGTTGCCGGCCACCAGTGTAAAATTTGATACATCCTTGTGATAAACACATAGAAGAAGACAACTCACACGTTGAGCAGTTTAAATTAATTTATAATTTTTTTTTTCCTC

mRNA sequence

CATTTCCTTTTCAGTTTCCAAATTCCAAACCCTAAACCTCCTTCTCTCTTCCTCTCTTTTCCATCCATTCCTCCATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACACCAAGCTTTCCGTCAGAAAACTCGCCGCCGACTCATTATACTACTTCTCTCTATTGTTCTTCTTATTGCTTTGGTCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGGAACTCTTCCTCTTCTTCGACCACGAGTTCGACTCCTCCCACTGAGCTCCCTCCGGCCGCTTCACTCAAAACTCTCTGTAGCGTCACTCAGTATCCGAGTTCCTGTCAATCCTCTCTCGAAAACTCCAACACCACCGATCCGATATTTCTCTTCAAGCTTTCTCTACGAGTCGCCACCGATTCGATCTCAAAGCTCTCTGATTATGCCTCTAACCTAAATTCCAGTACTAGTGATGTGAAGGTTAAAGCTGCGATTGGAATCTGCTCGTCTGTTTTTAAAGATGCCATTGACACACTAAACGACACCGTTTCATCCATGGAAGTGGATCGCCATGGCGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACAATCACAGACCAAGAAACCTGCCTCGATGCCATTCGAGATCTAAACCAGACCGCGGTTCTCGAAAATTTGCAAGCGGTAATGGCGAACTCGACCGAGTTCACTAGCAATAGTTTAGCGATTGTGACCAAAATCCTGGGGCTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGCGTTCCCGGAGTGGTTAAGCCCCGGCGATCGGAGGCTTTTGCAGGAGAACAATGTGACGGTGCATGCGACGGTGTCGAAGGATGGGACAGGGGATTTCACGACGATCAAAGAGGCCGTAGCAGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATCTATGTAAAAGAAGGATTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGCAAAACCAGAACCATTGTTTCAGGCCGCCTTAACTTCATCGACGGCACTCCCACCTACGCCACCGCCACTTTTGCTGTTCAAGGCAAAGGATTCATCGGGAAGGATATGGGGTTCATCAACACAGCCGGGCCGGCCAAACACCAGGCCGTCGCCTTCCGGTCAGGCTCCGACCTATCTGTGTTGTACGGATGTTCATTTGATGGCTATCAAGACACTCTTTACGCCCATTCAAACCGTCAATTCTACAGGAACTGCGACATCACCGGCACAATCGACTTCATCTTCGGCAACGCCGCCGTGGTATTCCAGAACTGCAACATCCGGCCCAGGCAGCCACTTCCCAATCAGTTCAACACCATCACAGCCCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCTGCCTACAACGACAGTCTCACTGCCCCCACTTACTTGGGGCGGCCATGGAAGGAATTTTCCACCACCGTGATTATGCAGTCGGAGATTGGGGGCTTCCTGCAGCCCATCGGGTGGAAGGAATGGGTGAGCGGGCAGGACCCACCCAGCAGCATATTTTACGGAGAGTATCAGAATAGTGGGCCTGGATCCAACGTGGACAAGAGGGTCAAATGGACCGGTTACAGGCCCAGTCTTACCGACGCCGAGGCCGCAAAGTTCACCGTCCGGACGTTCCTCAACGGCGAGGAATGGTTGCCGGCCACCAGTGTAAAATTTGATACATCCTTGTGATAAACACATAGAAGAAGACAACTCACACGTTGAGCAGTTTAAATTAATTTATAATTTTTTTTTTCCTC

Coding sequence (CDS)

ATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACACCAAGCTTTCCGTCAGAAAACTCGCCGCCGACTCATTATACTACTTCTCTCTATTGTTCTTCTTATTGCTTTGGTCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGGAACTCTTCCTCTTCTTCGACCACGAGTTCGACTCCTCCCACTGAGCTCCCTCCGGCCGCTTCACTCAAAACTCTCTGTAGCGTCACTCAGTATCCGAGTTCCTGTCAATCCTCTCTCGAAAACTCCAACACCACCGATCCGATATTTCTCTTCAAGCTTTCTCTACGAGTCGCCACCGATTCGATCTCAAAGCTCTCTGATTATGCCTCTAACCTAAATTCCAGTACTAGTGATGTGAAGGTTAAAGCTGCGATTGGAATCTGCTCGTCTGTTTTTAAAGATGCCATTGACACACTAAACGACACCGTTTCATCCATGGAAGTGGATCGCCATGGCGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACAATCACAGACCAAGAAACCTGCCTCGATGCCATTCGAGATCTAAACCAGACCGCGGTTCTCGAAAATTTGCAAGCGGTAATGGCGAACTCGACCGAGTTCACTAGCAATAGTTTAGCGATTGTGACCAAAATCCTGGGGCTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGCGTTCCCGGAGTGGTTAAGCCCCGGCGATCGGAGGCTTTTGCAGGAGAACAATGTGACGGTGCATGCGACGGTGTCGAAGGATGGGACAGGGGATTTCACGACGATCAAAGAGGCCGTAGCAGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATCTATGTAAAAGAAGGATTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGCAAAACCAGAACCATTGTTTCAGGCCGCCTTAACTTCATCGACGGCACTCCCACCTACGCCACCGCCACTTTTGCTGTTCAAGGCAAAGGATTCATCGGGAAGGATATGGGGTTCATCAACACAGCCGGGCCGGCCAAACACCAGGCCGTCGCCTTCCGGTCAGGCTCCGACCTATCTGTGTTGTACGGATGTTCATTTGATGGCTATCAAGACACTCTTTACGCCCATTCAAACCGTCAATTCTACAGGAACTGCGACATCACCGGCACAATCGACTTCATCTTCGGCAACGCCGCCGTGGTATTCCAGAACTGCAACATCCGGCCCAGGCAGCCACTTCCCAATCAGTTCAACACCATCACAGCCCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCTGCCTACAACGACAGTCTCACTGCCCCCACTTACTTGGGGCGGCCATGGAAGGAATTTTCCACCACCGTGATTATGCAGTCGGAGATTGGGGGCTTCCTGCAGCCCATCGGGTGGAAGGAATGGGTGAGCGGGCAGGACCCACCCAGCAGCATATTTTACGGAGAGTATCAGAATAGTGGGCCTGGATCCAACGTGGACAAGAGGGTCAAATGGACCGGTTACAGGCCCAGTCTTACCGACGCCGAGGCCGCAAAGTTCACCGTCCGGACGTTCCTCAACGGCGAGGAATGGTTGCCGGCCACCAGTGTAAAATTTGATACATCCTTGTGA

Protein sequence

MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSSSTTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKTWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIPIHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL

Homology

[Close]

BLAST of Carg06395 vs. NCBI nr
Match: KAG7018244.1 (Pectinesterase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

[Close]

BLAST of Carg06395 vs. NCBI nr
Match: XP_022956093.1 (pectinesterase 3-like [Cucurbita moschata] >KAG6581793.1 Pectinesterase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

[Close]

BLAST of Carg06395 vs. NCBI nr
Match: XP_023528356.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 570/576 (98.96%), Postives = 574/576 (99.65%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLS+VLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSVVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEWLSPGDRRLLQENNVT HATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTAHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIG FLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNV+KRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGAFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGE+WLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDTSL 576

[Close]

BLAST of Carg06395 vs. NCBI nr
Match: XP_022980574.1 (pectinesterase 3 [Cucurbita maxima])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 561/576 (97.40%), Postives = 572/576 (99.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGIC+SVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLN TAVLENLQAVMANSTEFTSNSLAIVTKILGLLA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEW+SPGDRRLLQENNVT HATVSKDGT ++TTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDG+TRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAA VFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQP+GWKEWVSGQDPPSSIFYGEYQNSGPGSNV+KRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGE+WLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

[Close]

BLAST of Carg06395 vs. NCBI nr
Match: XP_038877946.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 1046.2 bits (2704), Expect = 1.0e-301
Identity = 523/576 (90.80%), Postives = 549/576 (95.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLIILL+SIVLLIALVI AVVGIVIHKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLVSIVLLIALVIAAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           S TSS PPTEL P ASLKTLCSVTQYPSSCQSSLENSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  SATSSPPPTELTPLASLKTLCSVTQYPSSCQSSLENSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDY SNLNS+T D K+KAAI IC SVF+DAIDTLNDTVSSMEVDRH EKFLSPSRI+DLK
Sbjct: 121 SDYTSNLNSNTEDPKLKAAIEICRSVFEDAIDTLNDTVSSMEVDRHNEKFLSPSRIDDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL+NLQ  MANSTEFTSNSLAIVTKIL LLADF+IP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILRLLADFDIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLM FP+W+S GDRRLLQENNVT H TVSKDG G++TTIK+AVAAVPKKSK+RFIIY
Sbjct: 241 IHRKLMGFPDWVSSGDRRLLQENNVTAHVTVSKDGKGNYTTIKDAVAAVPKKSKQRFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+ GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVISGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYND+L APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDNLAAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQP+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWAG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEA KFTV TFLNGE+WLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAGKFTVGTFLNGEDWLPATSVKFDTSL 576

[Close]

BLAST of Carg06395 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 2.0e-207
Identity = 364/586 (62.12%), Postives = 457/586 (77.99%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MD++KSFKGYGKV+ELE QA+ +KTR+RLII+ +S ++LIA++I AV G+VIH RNS SS
Sbjct: 1   MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSC---QSSLENSNTTDPIFLFKLSLRVATDSI 120
            ++ S P TEL PAASLK +C  T+YPSSC    SSL  SNTTDP  LFKLSLRVA D  
Sbjct: 61  PSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAID-- 120

Query: 121 SKLSDYASNLNSST-SDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRI 180
            +LS + S L ++   D +++ AI +CSSVF DA+D LND++S++       +  S + +
Sbjct: 121 -ELSSFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISAL--GTVAGRIASSASV 180

Query: 181 EDLKTWLSTTITDQETCLDAIRDLNQTA---VLENLQAVMANSTEFTSNSLAIVTKILGL 240
            +++TWLS  +TDQ+TCLDA+ +LN TA    L+ ++  M NSTEF SNSLAIVTKILGL
Sbjct: 181 SNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 240

Query: 241 LADFNIPI-HRKLMAFPEWLSPGDRRLLQE--NNVTVHATVSKDGTGDFTTIKEAVAAVP 300
           L+ F  PI HR+L+ FPEWL   +RRLL+E  N+ T  A V+KDG+G F TI EA+  V 
Sbjct: 241 LSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVK 300

Query: 301 KKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFA 360
           KKS+ERF +YVKEG Y EN+ LDK+ WNVM+YGDGK +T V G  NF+DGTPT+ TATFA
Sbjct: 301 KKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFA 360

Query: 361 VQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNC 420
           V+GKGFI KD+GF+N AG +KHQAVA RSGSD SV + CSFDG+QDTLYAHSNRQFYR+C
Sbjct: 361 VKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDC 420

Query: 421 DITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN 480
           DITGTIDFIFGNAAVVFQ+C I PRQPLPNQFNTITAQGKKD NQN+GI IQK T + + 
Sbjct: 421 DITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFG 480

Query: 481 DSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGS 540
           ++LTAPTYLGRPWK+FSTTVIMQS+IG  L P+GW  WV   +PP++IFY EYQNSGPG+
Sbjct: 481 NNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGA 540

Query: 541 NVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           +V +RVKW GY+P++TD  A +FTV++F+ G EWLP  +V+FD++L
Sbjct: 541 DVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

[Close]

BLAST of Carg06395 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 9.8e-202
Identity = 360/590 (61.02%), Postives = 449/590 (76.10%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIA++I AVV  V+HK  + S 
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNES- 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSS---LENSNTTDPIFLFKLSLRVATDSI 120
              + +PP EL P+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D +
Sbjct: 61  ---TPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIE 180
             +SD    L+  T D ++K+A+ +C  + +DA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDAIRDLNQ-------TAVLENLQAVMANSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D++ +L Q       + + +NL++ M+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLADFNIPIH--RKLMA--------FPEWLSPGDRRLLQENNVTVHATVSKDGTGDFT 300
           L  L+D  IPIH  R+LM+        F +W     RRLLQ   +    TV+ DGTGD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKW---ARRRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKEAVAAVPKKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDG 360
           T+ EAVA VPKKS + F+IYVK G Y ENV++DKSKWNVM+YGDGK +TI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTYATATFAVQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYA 420
           TPTY TATFA+QGKGFI KD+G INTAG AKHQAVAFRSGSD SV Y CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRNCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYR+CD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFSAYNDSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFY 540
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP+SI Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGSNVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSV 571
           GEY+N+GPGS+V +RVKW GY+P ++DAEAAKFTV T L+G +W+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

[Close]

BLAST of Carg06395 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.1e-160
Identity = 293/528 (55.49%), Postives = 378/528 (71.59%), Query Frame = 0


Query: 52  IHKRNSSSSSTTSSTP-PTELPPAASLKTLCSVTQYPSSCQSSLE----NSNTTDPIFLF 111
           IH+R+   S     +P PT   P++ L+T+C+VT YP+SC SS+     +  TTDP  LF
Sbjct: 436 IHERHGVQSPRLRKSPHPT---PSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLF 495

Query: 112 KLSLRVATDSISKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSM-EVDR 171
           +LSL+V  D ++ +      L   T+D  +K+A+ +C+ VF  A+D++NDT+SS+ EV  
Sbjct: 496 RLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVIS 555

Query: 172 HGEKFLSPSRIEDLKTWLSTTITDQETCLDAIRDLN-QTAVLENLQAVMANSTEFTSNSL 231
            G+K L+ S I DL TWLS+ +TD  TC D + + N  + + + L++ M NSTEFTSNSL
Sbjct: 556 GGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSL 615

Query: 232 AIVTKILGLLADFNIPIH-RKLM---AFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFT 291
           AIV ++L   +   IP+  R+L+   +FP W+ PG RRLLQ  N+T H TV+ DG+GD  
Sbjct: 616 AIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVR 675

Query: 292 TIKEAVAAVPKKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDG 351
           T+ EAV  VPKK K  F+IYVK G Y ENV++ K KWNV +YGDG+ +TI+SG  N +DG
Sbjct: 676 TVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDG 735

Query: 352 TPTYATATFAVQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYA 411
             T+ T+TFA +GKGF+ KDMG INTAGP KHQAVAFRS SD SV Y CSFDGYQDTLY 
Sbjct: 736 VRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYT 795

Query: 412 HSNRQFYRNCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 471
           HSNRQ+YRNCD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GIS
Sbjct: 796 HSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGIS 855

Query: 472 IQKCTFSAYNDSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQD-PPSSIF 531
           I +CT S  N ++TA TYLGRPWK FS TVIMQS IG F+ P GW  W S  D PP +IF
Sbjct: 856 IHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIF 915

Query: 532 YGEYQNSGPGSNVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEE-WLP 567
           Y EY+NSGPGS++ KRVKW GY+P  +D EAA+FTV+ FL G++ W+P
Sbjct: 916 YREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

[Close]

BLAST of Carg06395 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.6e-135
Identity = 275/582 (47.25%), Postives = 377/582 (64.78%), Query Frame = 0


Query: 10  YGKVDELEHQAFR--QKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSSSTTSSTP 69
           YG++DE E       +KT++R+ I+ +S ++L+ +V+GAVVG        +++   S  P
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVG--------TTARDNSKKP 63

Query: 70  PTE---LPPAASLKTLCSVTQYPSSCQSSL---ENSNTTDPIFLFKLSLRVATDSISKLS 129
           PTE    P + S+K LC VT +   C  +L    N++ + P  LFK +++V    +SK+ 
Sbjct: 64  PTENNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVL 123

Query: 130 DYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKT 189
           D  S  N    D    AA+G C  +   A+D LN+T++S           S    +DL+T
Sbjct: 124 DGFS--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRT 183

Query: 190 WLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIPI 249
           WLS+  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG +AD     
Sbjct: 184 WLSSVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFR 243

Query: 250 HRKLM-------AFPEWLSPGDRRLLQENNVTVHAT--VSKDGTGDFTTIKEAVAAVPKK 309
            R+L+          +      RRLL+  ++   AT  V+KDG+G + TI EA+A V +K
Sbjct: 244 RRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEK 303

Query: 310 SKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQ 369
           +++  IIYVK+G+Y ENV ++K+KWNV+M GDG+++TIVS  LNFIDGTPT+ TATFAV 
Sbjct: 304 NEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVF 363

Query: 370 GKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDI 429
           GKGF+ +DMGFINTAGPAKHQAVA    +DLSV Y C+ D +QDT+YAH+ RQFYR+C I
Sbjct: 364 GKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVI 423

Query: 430 TGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDS 489
            GT+DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++ 
Sbjct: 424 LGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNL 483

Query: 490 LTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNV 549
               T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P +IFY EY NSGPG++ 
Sbjct: 484 TDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGAST 543

Query: 550 DKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDT 575
             RVKW G + SLT  EA KFTV+ F++G  WLPAT V F++
Sbjct: 544 KNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Carg06395 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.9e-129
Identity = 262/577 (45.41%), Postives = 374/577 (64.82%), Query Frame = 0


Query: 10  YGKVDELEHQAF--RQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSSSTTSSTP 69
           YGKVDE EH     R+KTR+ + I+ +S+V+L  +VIGAV G + HK++  +  T ++  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELPPAASLKTLCSVTQYPSSCQSSL---ENSNTTDPIFLFKLSLRVATDSISKLSDYA 129
                 + S+K +C VT +   C  +L    N+++ +P  LF+ ++++   +I+++S   
Sbjct: 65  SI----SVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 130 SNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKTWLS 189
           +  +SS  D K    +  C+ +    ID LN+T++S     +G+  + P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTS---SSNGDVTV-PELVDDLRTWLS 184

Query: 190 TTITDQETCLDAIR-DLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIPIHR 249
           +  T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG +AD +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 250 KLM--AFPEWLSPGDRRLLQENNV--TVHATVSKDGTGDFTTIKEAVAAVPKKSKERFII 309
           +L+  A  E      RRLLQ  ++       V+KDG+G + TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 310 YVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGK 369
           YVK+G+Y ENV ++K  WNV++ GDG++++IVSGRLN IDGTPT+ TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 370 DMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFI 429
           DMGFINTAGP+KHQAVA    +DL+  Y C+ + YQDTLY H+ RQFYR C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 430 FGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYL 489
           FGN+A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  S   D     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 490 GRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWT 549
           GRPWK FSTTVIM S + GF+   GW  W +G   P +IFYGEY+N+GPG++   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 550 GYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           G R  L+  EA +FTV+ F++G  WLPAT V F + L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Carg06395 vs. ExPASy TrEMBL
Match: A0A6J1GVT9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

[Close]

BLAST of Carg06395 vs. ExPASy TrEMBL
Match: A0A6J1IZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 561/576 (97.40%), Postives = 572/576 (99.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDYASNLNSSTSDVKVKAAIGIC+SVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDAIRDLN TAVLENLQAVMANSTEFTSNSLAIVTKILGLLA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLMAFPEW+SPGDRRLLQENNVT HATVSKDGT ++TTIKEAVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEGLYEENVILDKSKWNVMMYGDG+TRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAA VFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFLQP+GWKEWVSGQDPPSSIFYGEYQNSGPGSNV+KRVKWTG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           YRPSLTDAEAAKFTVRTFLNGE+WLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

[Close]

BLAST of Carg06395 vs. ExPASy TrEMBL
Match: A0A1S3CBU9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.0e-298
Identity = 511/576 (88.72%), Postives = 547/576 (94.97%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDY SN NS+T D KVKAAI IC SVF+DAIDTLNDT+SSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL++LQ  MANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLM  PEW+S GDRRLLQENNVT H TVSKDG GD+TTIK+AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+ GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFL P+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           Y+PSLTD+EA KFTV TFLNGE+WLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Carg06395 vs. ExPASy TrEMBL
Match: A0A5A7UGG5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970 PE=3 SV=1)

HSP 1 Score: 1032.7 bits (2669), Expect = 5.8e-298
Identity = 510/576 (88.54%), Postives = 546/576 (94.79%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDY SN NS+T D KVKAAI IC SVF+DAIDTLNDT+SSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL+ LQ  MANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLM  PEW+S GDR+LLQENNVT H TVSKDG GD+TTIK+AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+ GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIMQSEIGGFL P+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           Y+PSLTD+EA KFTV TFLNGE+WLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Carg06395 vs. ExPASy TrEMBL
Match: A0A0A0L956 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.4e-296
Identity = 507/576 (88.02%), Postives = 545/576 (94.62%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180
           SDY SN NS+T D KV+AAI IC SVF+DAIDTLNDTVSSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL++LQ  MANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300
           IHRKLM  PEW+S GDRRLLQENNVT H TVSKDG G +TTI++AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+ GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540
           RPWKEFSTTVIM+SEIGGFL P+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           Y+PSLTD+EA +FTV TFLNGE+WLPAT+V FDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

[Close]

BLAST of Carg06395 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 704.5 bits (1817), Expect = 6.9e-203
Identity = 360/590 (61.02%), Postives = 449/590 (76.10%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIA++I AVV  V+HK  + S 
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNES- 60

Query: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSS---LENSNTTDPIFLFKLSLRVATDSI 120
              + +PP EL P+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D +
Sbjct: 61  ---TPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIE 180
             +SD    L+  T D ++K+A+ +C  + +DA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDAIRDLNQ-------TAVLENLQAVMANSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D++ +L Q       + + +NL++ M+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLADFNIPIH--RKLMA--------FPEWLSPGDRRLLQENNVTVHATVSKDGTGDFT 300
           L  L+D  IPIH  R+LM+        F +W     RRLLQ   +    TV+ DGTGD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKW---ARRRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKEAVAAVPKKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDG 360
           T+ EAVA VPKKS + F+IYVK G Y ENV++DKSKWNVM+YGDGK +TI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTYATATFAVQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYA 420
           TPTY TATFA+QGKGFI KD+G INTAG AKHQAVAFRSGSD SV Y CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRNCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYR+CD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFSAYNDSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFY 540
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP+SI Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGSNVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSV 571
           GEY+N+GPGS+V +RVKW GY+P ++DAEAAKFTV T L+G +W+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

[Close]

BLAST of Carg06395 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 565.5 bits (1456), Expect = 5.0e-161
Identity = 293/528 (55.49%), Postives = 378/528 (71.59%), Query Frame = 0


Query: 52  IHKRNSSSSSTTSSTP-PTELPPAASLKTLCSVTQYPSSCQSSLE----NSNTTDPIFLF 111
           IH+R+   S     +P PT   P++ L+T+C+VT YP+SC SS+     +  TTDP  LF
Sbjct: 436 IHERHGVQSPRLRKSPHPT---PSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLF 495

Query: 112 KLSLRVATDSISKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSM-EVDR 171
           +LSL+V  D ++ +      L   T+D  +K+A+ +C+ VF  A+D++NDT+SS+ EV  
Sbjct: 496 RLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVIS 555

Query: 172 HGEKFLSPSRIEDLKTWLSTTITDQETCLDAIRDLN-QTAVLENLQAVMANSTEFTSNSL 231
            G+K L+ S I DL TWLS+ +TD  TC D + + N  + + + L++ M NSTEFTSNSL
Sbjct: 556 GGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSL 615

Query: 232 AIVTKILGLLADFNIPIH-RKLM---AFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFT 291
           AIV ++L   +   IP+  R+L+   +FP W+ PG RRLLQ  N+T H TV+ DG+GD  
Sbjct: 616 AIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVR 675

Query: 292 TIKEAVAAVPKKSKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDG 351
           T+ EAV  VPKK K  F+IYVK G Y ENV++ K KWNV +YGDG+ +TI+SG  N +DG
Sbjct: 676 TVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDG 735

Query: 352 TPTYATATFAVQGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYA 411
             T+ T+TFA +GKGF+ KDMG INTAGP KHQAVAFRS SD SV Y CSFDGYQDTLY 
Sbjct: 736 VRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYT 795

Query: 412 HSNRQFYRNCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 471
           HSNRQ+YRNCD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GIS
Sbjct: 796 HSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGIS 855

Query: 472 IQKCTFSAYNDSLTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQD-PPSSIF 531
           I +CT S  N ++TA TYLGRPWK FS TVIMQS IG F+ P GW  W S  D PP +IF
Sbjct: 856 IHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIF 915

Query: 532 YGEYQNSGPGSNVDKRVKWTGYRPSLTDAEAAKFTVRTFLNGEE-WLP 567
           Y EY+NSGPGS++ KRVKW GY+P  +D EAA+FTV+ FL G++ W+P
Sbjct: 916 YREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

HSP 2 Score: 200.7 bits (509), Expect = 3.3e-51
Identity = 123/253 (48.62%), Postives = 171/253 (67.59%), Query Frame = 0


Query: 1   MDSVKSF-KGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSS 60
           MD+VKS  KGYGKVDE +  A ++KTR+RL  + +S+ +L+A++I + V I IH R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 61  SSTTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLEN---SNTTDPIFLFKLSLRVATDS 120
              T S+ P EL PAASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 121 ISKLSDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRI 180
           ++ + +    L   T D  +K+A+ +C  +   AID +N+TVS+MEV   G+K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 181 EDLKTWLSTTITDQETCLDAIRDLNQT--AVLENLQAVMANSTEFTSNSLAIVTKILGLL 240
           +DL TWLS  +T   TCLDA+ +++ T  A+   L++ M NSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 241 ADFNIPIH-RKLM 247
           +DF IPIH R+L+
Sbjct: 241 SDFGIPIHGRRLL 251

[Close]

BLAST of Carg06395 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 484.6 bits (1246), Expect = 1.1e-136
Identity = 275/582 (47.25%), Postives = 377/582 (64.78%), Query Frame = 0


Query: 10  YGKVDELEHQAFR--QKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSSSTTSSTP 69
           YG++DE E       +KT++R+ I+ +S ++L+ +V+GAVVG        +++   S  P
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVG--------TTARDNSKKP 63

Query: 70  PTE---LPPAASLKTLCSVTQYPSSCQSSL---ENSNTTDPIFLFKLSLRVATDSISKLS 129
           PTE    P + S+K LC VT +   C  +L    N++ + P  LFK +++V    +SK+ 
Sbjct: 64  PTENNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVL 123

Query: 130 DYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKT 189
           D  S  N    D    AA+G C  +   A+D LN+T++S           S    +DL+T
Sbjct: 124 DGFS--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRT 183

Query: 190 WLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIPI 249
           WLS+  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG +AD     
Sbjct: 184 WLSSVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFR 243

Query: 250 HRKLM-------AFPEWLSPGDRRLLQENNVTVHAT--VSKDGTGDFTTIKEAVAAVPKK 309
            R+L+          +      RRLL+  ++   AT  V+KDG+G + TI EA+A V +K
Sbjct: 244 RRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEK 303

Query: 310 SKERFIIYVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQ 369
           +++  IIYVK+G+Y ENV ++K+KWNV+M GDG+++TIVS  LNFIDGTPT+ TATFAV 
Sbjct: 304 NEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVF 363

Query: 370 GKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDI 429
           GKGF+ +DMGFINTAGPAKHQAVA    +DLSV Y C+ D +QDT+YAH+ RQFYR+C I
Sbjct: 364 GKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVI 423

Query: 430 TGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDS 489
            GT+DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++ 
Sbjct: 424 LGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNL 483

Query: 490 LTAPTYLGRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNV 549
               T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P +IFY EY NSGPG++ 
Sbjct: 484 TDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGAST 543

Query: 550 DKRVKWTGYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDT 575
             RVKW G + SLT  EA KFTV+ F++G  WLPAT V F++
Sbjct: 544 KNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Carg06395 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.5e-130
Identity = 262/577 (45.41%), Postives = 374/577 (64.82%), Query Frame = 0


Query: 10  YGKVDELEHQAF--RQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSSSTTSSTP 69
           YGKVDE EH     R+KTR+ + I+ +S+V+L  +VIGAV G + HK++  +  T ++  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELPPAASLKTLCSVTQYPSSCQSSL---ENSNTTDPIFLFKLSLRVATDSISKLSDYA 129
                 + S+K +C VT +   C  +L    N+++ +P  LF+ ++++   +I+++S   
Sbjct: 65  SI----SVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 130 SNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKTWLS 189
           +  +SS  D K    +  C+ +    ID LN+T++S     +G+  + P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTS---SSNGDVTV-PELVDDLRTWLS 184

Query: 190 TTITDQETCLDAIR-DLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIPIHR 249
           +  T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG +AD +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 250 KLM--AFPEWLSPGDRRLLQENNV--TVHATVSKDGTGDFTTIKEAVAAVPKKSKERFII 309
           +L+  A  E      RRLLQ  ++       V+KDG+G + TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 310 YVKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGK 369
           YVK+G+Y ENV ++K  WNV++ GDG++++IVSGRLN IDGTPT+ TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 370 DMGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFI 429
           DMGFINTAGP+KHQAVA    +DL+  Y C+ + YQDTLY H+ RQFYR C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 430 FGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYL 489
           FGN+A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  S   D     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 490 GRPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWT 549
           GRPWK FSTTVIM S + GF+   GW  W +G   P +IFYGEY+N+GPG++   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 550 GYRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
           G R  L+  EA +FTV+ F++G  WLPAT V F + L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Carg06395 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 401.7 bits (1031), Expect = 9.6e-112
Identity = 217/510 (42.55%), Postives = 303/510 (59.41%), Query Frame = 0


Query: 79  TLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKLSDYASNLNSSTSDVKVKA 138
           +LCS  +   S    ++      P   F  + +   D+I+K     S  +      +V  
Sbjct: 20  SLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSN 79

Query: 139 AIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLKTWLSTTITDQETCLDAIR 198
           AI  C  +   A + L+  +S+ +   +G+   +     DL+TW+S  +++Q+TCLD   
Sbjct: 80  AIVDCVDLLDSAAEELSWIISASQ-SPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 139

Query: 199 DLNQTAVLENLQAVMANSTEFTSNSLAIVT-------KILGLLADFNIPIHRKLMAFPEW 258
             N   +++ + A   +    T  +L  +        K   + A      H     FP W
Sbjct: 140 GTN--GIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSW 199

Query: 259 LSPGDRRLLQENNVTV-HATVSKDGTGDFTTIKEAVAAVPKKSKERFIIYVKEGLYEENV 318
           + PGDR+LLQ +N+TV  A V+ DGTG+FTTI +AV A P  S +R++I+VK G+Y ENV
Sbjct: 200 VKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENV 259

Query: 319 ILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKDMGFINTAGPA 378
            + K KWN+MM GDG   T+++G  +FIDG  T+ +ATFAV G+GFI +D+ F NTAGP 
Sbjct: 260 EIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPE 319

Query: 379 KHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIFGNAAVVFQNC 438
           KHQAVA RS +DL V Y C+  GYQDTLYAHS RQF+R C ITGT+DFIFG+A  VFQ+C
Sbjct: 320 KHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSC 379

Query: 439 NIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL----TAPTYLGRPWKEF 498
            I+ +Q LPNQ N+ITAQG+KD N+ +G +IQ    +A  D L    T  TYLGRPWK +
Sbjct: 380 QIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLY 439

Query: 499 STTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTGYRPSLT 558
           S TV MQ+ +   + P+GW EW +G     +++YGEY NSGPG+++D+RVKW GY    T
Sbjct: 440 SRTVFMQNYMSDAINPVGWLEW-NGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNT 499

Query: 559 DAEAAKFTVRTFLNGEEWLPATSVKFDTSL 577
            AEA  FTV   + G  WLP+T + F   L
Sbjct: 500 SAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:

BLAST of Carg06395 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018244.1	0.0e+00	100.00	Pectinesterase 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022956093.1	0.0e+00	99.83	pectinesterase 3-like [Cucurbita moschata] >KAG6581793.1 Pectinesterase 3, parti...	[more]
XP_023528356.1	0.0e+00	98.96	pectinesterase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980574.1	0.0e+00	97.40	pectinesterase 3 [Cucurbita maxima]	[more]
XP_038877946.1	1.0e-301	90.80	pectinesterase 3 [Benincasa hispida]	[more]

BLAST of Carg06395 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43111	2.0e-207	62.12	Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1	[more]
Q43867	9.8e-202	61.02	Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1	[more]
Q9LUL8	7.1e-160	55.49	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF78	1.6e-135	47.25	Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3...	[more]
Q9SG77	4.9e-129	45.41	Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg06395 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GVT9	0.0e+00	99.83	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1	[more]
A0A6J1IZN0	0.0e+00	97.40	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1	[more]
A0A1S3CBU9	2.0e-298	88.72	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1	[more]
A0A5A7UGG5	5.8e-298	88.54	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970...	[more]
A0A0A0L956	1.4e-296	88.02	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1	[more]

BLAST of Carg06395 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53840.1	6.9e-203	61.02	pectin methylesterase 1	[more]
AT3G14300.1	5.0e-161	55.49	pectinesterase family protein	[more]
AT5G04960.1	1.1e-136	47.25	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10710.1	3.5e-130	45.41	root hair specific 12	[more]
AT3G43270.1	9.6e-112	42.55	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 72..226 e-value: 2.4E-32 score: 123.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 77..226 e-value: 3.3E-24 score: 85.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 57..230 e-value: 1.0E-18 score: 65.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 75..231 e-value: 2.6E-30 score: 107.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 77..231
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 262..576 e-value: 2.9E-115 score: 387.0
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 269..561 e-value: 1.0E-138 score: 461.1
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 8..573
None	No IPR available	PANTHER	PTHR31707:SF219	PECTINESTERASE 1-RELATED	coord: 8..573
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 79..230 e-value: 2.17468E-41 score: 144.888
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 412..421
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 268..572

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06395-RA	Carg06395-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity