Carg06309 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06309
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein RALF-like 24
Location	Carg_Chr06: 7892709 .. 7894718 (+)
RNA-Seq Expression	Carg06309
Synteny	Carg06309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAACCCACCACTCTGTTCTTCCTTCTTTCCATCCTCTTCCTTCCACTCACTTTCCTTCAAACCCTTTTCCCCTTGTGCGATGCTGTTCTTGATCTCAGATCTTCGAAGGGCAACCAAATCGATTTAATACCAAAAGGGGTTTGCAATCAGAAGATCGGAGAGTGCTTAACAGAGCCAGAAATGGAATCGGAGATCAGTAGGCGAGTGTTGATGATGCAGAAGAGGTATATAAGCTATGAAACTCTTAGAAGGGATATGGTTCCTTGTTCTAGACCAGGGGTTTCTTATTATGAATGCCATCCTGGTCCGGCGAATTCGTATGATCGTGGGTGCGAGGTTATTACTAGATGTGCTAGAGATGTGCACGATGTAAAGACTTGAAGGCGATGGCGAAGGTAATGAATGTGAGGAATGGTGTTTGATTTTGATTTTGATTTTGATTTGGTTTTTGAAATGTGGGTTTGATTTATTGTTGTTGATTTGGGTTTGGATTGGTGTGTTCATGTGCTGCATTGTTGATTTGTGATGAGATCTGGGGTTTCTATGGTTTTGTTTAGTGGGGTCTGTGATTTTGTGTGGTATCACTTGATTTAGCCATAAGGGGTTTGTGTATATGTGATGAGATATGGTCTGTTTTGGCTCGTTTACGTATAGTTGTTAGCCTCACCTTAGCTCTTGTAACATCCAAAGCCTATTGCTAGTAGATGTTGTCCGCTTTGGCCCGTTACGTGTCGCCGTCAGTCTCACCTAAGCTCTTGTAACAACCAAAGTCCACCGTTAGGAGATATTGTTTGTTTTGGCTCGTTACATATAGTTGTCAGCCTCACCTTATCTCTTGTAAGAGCCAAAGCCCACTGCTAGTAGATGTTGTCCGTTTTGGCCCGTTACGTATCGCCGTCAGTCTCACCTAAGCTCTTGTAACAACTAAAACCCACCGCTTGTAGATATTGTCTGCTTTTGCCTGTTATGTATCGCCGTCAGTCTCATGGTTTTAAAACGTGGTTTCCCAACCAATGTGAGATCTCACAATCCACCCCTTGGAAGCCTAGCATCCTCGCTGGCATACCACCCAATGACTGGCTCTGGCTTTGTAACAGCCCAAGCCCATCGCTAGTAGATACTGTCTGTTTTAGCCCGTTACATATCGTCATCGCTAGAACACTGCCTAGTGACGGGCTCTTATACCATTTGTAAGAGTCCAACTCCATTGCTAGTAGATATTGTCCAGCCCGTTACATATCGCCATCACTAGGGGCTAGCGTTATCGCTAGAACACTGCCTAGTGACGGACTCTTATACCATTTGTAAGAGTCCAAGTCCATTGCTAGTAGATATTGTCTGCTTTGGCCCGTTACACCTCTTGGTTGCTTTTCTGGTCCTTGCATATACTTGCTTCATATCTCTCAAGGAATTGCGAATTCTTTCGAGAAACGGCTACTATAGGAAAAAGTGTTGTAGAAGAGCTAGCCATTTTCATGAAACAGAACCTTCGAGTCGAGAAAGGTTGAGATATATATCTCCTAGGGCGTATTTCGTTAGTTAAACAGGACTAAGAACGGCCCATTCCAAAGGGAGCAGAGAGAGATTTATCTCCATTAGTTAAACGGGGTTGAGAATGGCCCATTCCCGCCCACCCGCTTTTTACAAATAGAGATGGCGGGACCGAGAGGTCAAAGCTCGGAGGTTTATCCAAGCTCCTCCAAACGTTGACTTCATTATGAAGTTCATTATTTGTGTGATGGGGATTGCTGTTTAGGAGTTTCAGACAAGACATTTTTTTCTTAAACTGTGATTGATTCTGAGAATGTGTAATTCTACAGCTAGCTGCTGGCAGCCGTTCGACTGTTGTACCGTAAGCAGGCATCGACAGGAGGTCGTTGTTCATCGAGATCAAGCAAGAAGAACATCCGTGTGCTTCTGTAAAAAGTATTGTTGAATATTATGAAAGCTTGAACAACTGATGTTATCATAGCTTCATTTTAAATGGAATTTCTATTGAACACC

mRNA sequence

ATGGCAAAACCCACCACTCTGTTCTTCCTTCTTTCCATCCTCTTCCTTCCACTCACTTTCCTTCAAACCCTTTTCCCCTTGTGCGATGCTGTTCTTGATCTCAGATCTTCGAAGGGCAACCAAATCGATTTAATACCAAAAGGGGTTTGCAATCAGAAGATCGGAGAGTGCTTAACAGAGCCAGAAATGGAATCGGAGATCAGTAGGCGAGTGTTGATGATGCAGAAGAGGTATATAAGCTATGAAACTCTTAGAAGGGATATGGTTCCTTGTTCTAGACCAGGGGTTTCTTATTATGAATGCCATCCTGGTCCGGCGAATTCGTATGATCGTGGGTGCGAGGTTATTACTAGATGTGCTAGAGATGTGCACGATCTAGCTGCTGGCAGCCGTTCGACTGTTGTACCGTAAGCAGGCATCGACAGGAGGTCGTTGTTCATCGAGATCAAGCAAGAAGAACATCCGTGTGCTTCTGTAAAAAGTATTGTTGAATATTATGAAAGCTTGAACAACTGATGTTATCATAGCTTCATTTTAAATGGAATTTCTATTGAACACC

Coding sequence (CDS)

ATGGCAAAACCCACCACTCTGTTCTTCCTTCTTTCCATCCTCTTCCTTCCACTCACTTTCCTTCAAACCCTTTTCCCCTTGTGCGATGCTGTTCTTGATCTCAGATCTTCGAAGGGCAACCAAATCGATTTAATACCAAAAGGGGTTTGCAATCAGAAGATCGGAGAGTGCTTAACAGAGCCAGAAATGGAATCGGAGATCAGTAGGCGAGTGTTGATGATGCAGAAGAGGTATATAAGCTATGAAACTCTTAGAAGGGATATGGTTCCTTGTTCTAGACCAGGGGTTTCTTATTATGAATGCCATCCTGGTCCGGCGAATTCGTATGATCGTGGGTGCGAGGTTATTACTAGATGTGCTAGAGATGTGCACGATCTAGCTGCTGGCAGCCGTTCGACTGTTGTACCGTAA

Protein sequence

MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTEPEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDLAAGSRSTVVP

Homology

[Close]

BLAST of Carg06309 vs. NCBI nr
Match: KAG7028741.1 (Protein RALF-like 31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277.3 bits (708), Expect = 6.9e-71
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE
Sbjct: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDLAAGSRSTVVP 137
           RDVHDLAAGSRSTVVP
Sbjct: 121 RDVHDLAAGSRSTVVP 136

[Close]

BLAST of Carg06309 vs. NCBI nr
Match: XP_022946007.1 (protein RALF-like 24 [Cucurbita moschata])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 123/126 (97.62%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKPTTLFFLLSIL LPLTFLQTLFPLCDAVLDLRS KGNQIDLIPKGVCNQKIGECLTE
Sbjct: 1   MAKPTTLFFLLSILLLPLTFLQTLFPLCDAVLDLRSLKGNQIDLIPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDV 126

[Close]

BLAST of Carg06309 vs. NCBI nr
Match: XP_023539976.1 (protein RALF-like 31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.1 bits (640), Expect = 5.3e-63
Identity = 121/126 (96.03%), Postives = 123/126 (97.62%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKPTTLFFLLSIL LPLTFLQTLFP CDAVLDLRS KGNQIDL+PKGVCNQKIGECLTE
Sbjct: 1   MAKPTTLFFLLSILLLPLTFLQTLFPFCDAVLDLRSLKGNQIDLMPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 126

[Close]

BLAST of Carg06309 vs. NCBI nr
Match: XP_022974738.1 (protein RALF-like 24 [Cucurbita maxima])

HSP 1 Score: 234.2 bits (596), Expect = 6.7e-58
Identity = 117/126 (92.86%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKP TLFFLLSIL LPL FLQT FP CDAVLDLRS KGNQIDL+PKGVCNQKIGECLTE
Sbjct: 1   MAKP-TLFFLLSIL-LPLIFLQTHFPFCDAVLDLRSLKGNQIDLMPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           P+MESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PQMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 124

[Close]

BLAST of Carg06309 vs. NCBI nr
Match: KAG6597269.1 (Protein RALF-like 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 217.2 bits (552), Expect = 8.5e-53
Identity = 114/136 (83.82%), Postives = 116/136 (85.29%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE
Sbjct: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHP                 
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPD---------------L 120

Query: 121 RDVHDLAAGSRSTVVP 137
           + +  LAAGSRSTVVP
Sbjct: 121 KAMAKLAAGSRSTVVP 121

[Close]

BLAST of Carg06309 vs. ExPASy Swiss-Prot
Match: Q9LK37 (Protein RALF-like 24 OS=Arabidopsis thaliana OX=3702 GN=RALFL24 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-23
Identity = 62/116 (53.45%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 11  LSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTEPEMESEISRR 70
           L++++L L  LQT   +    + + S  G +ID +     N  IGE   E  M SEISRR
Sbjct: 5   LALVYLSLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEEMMPSEISRR 64

Query: 71  VLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDL 127
           V+MM+K+YISYETLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCARD +D+
Sbjct: 65  VMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCARDTNDI 116

[Close]

BLAST of Carg06309 vs. ExPASy Swiss-Prot
Match: Q2HIM9 (Protein RALF-like 31 OS=Arabidopsis thaliana OX=3702 GN=RALFL31 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.8e-22
Identity = 62/122 (50.82%), Postives = 77/122 (63.11%), Query Frame = 0


Query: 5   TTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTEPEME 64
           +T   + +ILFL ++     FP+         S   +ID +   + N  IGE   E  M 
Sbjct: 4   STALVIFAILFLLIS--ADAFPI--------PSPNGEIDAML--IRNSIIGE--DEDLMP 63

Query: 65  SEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVH 124
           +EISRRVLM QKRYI YETLRRDMVPC +PG SYY+C  G ANSY RGC+ ITRCARD +
Sbjct: 64  TEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCRSGQANSYSRGCDTITRCARDTN 111

Query: 125 DL 127
           D+
Sbjct: 124 DI 111

[Close]

BLAST of Carg06309 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.5e-15
Identity = 45/93 (48.39%), Postives = 55/93 (59.14%), Query Frame = 0


Query: 39  GNQIDLIPKGV------CNQKIGECLTEP---EMESEISRRVLMMQKRYISYETLRRDMV 98
           G+ +D +  G       C   I EC+ E    E +S+ISRR+L  QK+YISY  +RR+ V
Sbjct: 28  GDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNSV 87

Query: 99  PCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 122
           PCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 88  PCSRRGASYYNCQRGAQANPYSRGCSTITRCRR 119

[Close]

BLAST of Carg06309 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.6e-15
Identity = 41/76 (53.95%), Postives = 50/76 (65.79%), Query Frame = 0


Query: 48  GVCNQKIGECLTEP---EMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPG 107
           G C   IGEC+ E    E++SE +RR+L   K+YISY  L+++ VPCSR G SYY C PG
Sbjct: 39  GGCKGSIGECIAEEEEFELDSESNRRIL-ATKKYISYGALQKNSVPCSRRGASYYNCKPG 98

Query: 108 -PANSYDRGCEVITRC 120
             AN Y RGC  ITRC
Sbjct: 99  AQANPYSRGCSAITRC 113

[Close]

BLAST of Carg06309 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-14
Identity = 46/86 (53.49%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 43  DLIP-KGVCNQKIGECL-----TEPEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGV 102
           D +P +  CN  I EC       E EM+SEI+RR+L   K YISY  LRR+ VPCSR G 
Sbjct: 32  DFVPIESKCNGTIAECSLSTAEEEFEMDSEINRRILATTK-YISYGALRRNTVPCSRRGA 91

Query: 103 SYYECHPG-PANSYDRGCEVITRCAR 122
           SYY C  G  AN Y RGC  ITRC R
Sbjct: 92  SYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Carg06309 vs. ExPASy TrEMBL
Match: A0A6J1G2H2 (protein RALF-like 24 OS=Cucurbita moschata OX=3662 GN=LOC111450186 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.2e-64
Identity = 123/126 (97.62%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKPTTLFFLLSIL LPLTFLQTLFPLCDAVLDLRS KGNQIDLIPKGVCNQKIGECLTE
Sbjct: 1   MAKPTTLFFLLSILLLPLTFLQTLFPLCDAVLDLRSLKGNQIDLIPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDV 126

[Close]

BLAST of Carg06309 vs. ExPASy TrEMBL
Match: A0A6J1IH73 (protein RALF-like 24 OS=Cucurbita maxima OX=3661 GN=LOC111473473 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.3e-58
Identity = 117/126 (92.86%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAKP TLFFLLSIL LPL FLQT FP CDAVLDLRS KGNQIDL+PKGVCNQKIGECLTE
Sbjct: 1   MAKP-TLFFLLSIL-LPLIFLQTHFPFCDAVLDLRSLKGNQIDLMPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           P+MESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PQMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 124

[Close]

BLAST of Carg06309 vs. ExPASy TrEMBL
Match: A0A0A0L7V8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G129640 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.2e-52
Identity = 103/126 (81.75%), Postives = 115/126 (91.27%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAK   LFFL SI+F PL FLQT F  CDA+LDLRS KG++++++ KGVCNQKIGECLT+
Sbjct: 1   MAKSLFLFFLFSIIF-PLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQK+YISY+TLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 125

[Close]

BLAST of Carg06309 vs. ExPASy TrEMBL
Match: A0A1S3AWQ1 (protein RALF-like 24 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.7e-52
Identity = 101/126 (80.16%), Postives = 112/126 (88.89%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAK   LFF L  +  PL FLQT F  CDA+LDLRS KG+++D++ KGVCNQKIGECLT+
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQK+YISY+TLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 126

[Close]

BLAST of Carg06309 vs. ExPASy TrEMBL
Match: A0A5D3CZU4 (Protein RALF-like 24 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002970 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.7e-52
Identity = 101/126 (80.16%), Postives = 112/126 (88.89%), Query Frame = 0


Query: 1   MAKPTTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTE 60
           MAK   LFF L  +  PL FLQT F  CDA+LDLRS KG+++D++ KGVCNQKIGECLT+
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQK+YISY+TLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDL 127
           RDVHD+
Sbjct: 121 RDVHDI 126

[Close]

BLAST of Carg06309 vs. TAIR 10
Match: AT3G23805.1 (ralf-like 24 )

HSP 1 Score: 110.9 bits (276), Expect = 8.0e-25
Identity = 62/116 (53.45%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 11  LSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTEPEMESEISRR 70
           L++++L L  LQT   +    + + S  G +ID +     N  IGE   E  M SEISRR
Sbjct: 5   LALVYLSLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEEMMPSEISRR 64

Query: 71  VLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDL 127
           V+MM+K+YISYETLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCARD +D+
Sbjct: 65  VMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCARDTNDI 116

[Close]

BLAST of Carg06309 vs. TAIR 10
Match: AT4G13950.1 (ralf-like 31 )

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-23
Identity = 62/122 (50.82%), Postives = 77/122 (63.11%), Query Frame = 0


Query: 5   TTLFFLLSILFLPLTFLQTLFPLCDAVLDLRSSKGNQIDLIPKGVCNQKIGECLTEPEME 64
           +T   + +ILFL ++     FP+         S   +ID +   + N  IGE   E  M 
Sbjct: 4   STALVIFAILFLLIS--ADAFPI--------PSPNGEIDAML--IRNSIIGE--DEDLMP 63

Query: 65  SEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVH 124
           +EISRRVLM QKRYI YETLRRDMVPC +PG SYY+C  G ANSY RGC+ ITRCARD +
Sbjct: 64  TEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCRSGQANSYSRGCDTITRCARDTN 111

Query: 125 DL 127
           D+
Sbjct: 124 DI 111

[Close]

BLAST of Carg06309 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 83.2 bits (204), Expect = 1.8e-16
Identity = 45/93 (48.39%), Postives = 55/93 (59.14%), Query Frame = 0


Query: 39  GNQIDLIPKGV------CNQKIGECLTEP---EMESEISRRVLMMQKRYISYETLRRDMV 98
           G+ +D +  G       C   I EC+ E    E +S+ISRR+L  QK+YISY  +RR+ V
Sbjct: 28  GDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNSV 87

Query: 99  PCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 122
           PCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 88  PCSRRGASYYNCQRGAQANPYSRGCSTITRCRR 119

[Close]

BLAST of Carg06309 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 80.5 bits (197), Expect = 1.2e-15
Identity = 46/86 (53.49%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 43  DLIP-KGVCNQKIGECL-----TEPEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGV 102
           D +P +  CN  I EC       E EM+SEI+RR+L   K YISY  LRR+ VPCSR G 
Sbjct: 32  DFVPIESKCNGTIAECSLSTAEEEFEMDSEINRRILATTK-YISYGALRRNTVPCSRRGA 91

Query: 103 SYYECHPG-PANSYDRGCEVITRCAR 122
           SYY C  G  AN Y RGC  ITRC R
Sbjct: 92  SYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Carg06309 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 78.2 bits (191), Expect = 5.7e-15
Identity = 40/73 (54.79%), Postives = 49/73 (67.12%), Query Frame = 0


Query: 50  CNQKIGECL--TEPEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPG-PA 109
           C+  I EC+   E EM+SEI+RR+L   K YISY++L+R+ VPCSR G SYY C  G  A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 110 NSYDRGCEVITRC 120
           N Y RGC  I RC
Sbjct: 107 NPYSRGCSKIARC 118

The following BLAST results are available for this feature:

BLAST of Carg06309 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028741.1	6.9e-71	100.00	Protein RALF-like 31, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022946007.1	1.1e-63	97.62	protein RALF-like 24 [Cucurbita moschata]	[more]
XP_023539976.1	5.3e-63	96.03	protein RALF-like 31 [Cucurbita pepo subsp. pepo]	[more]
XP_022974738.1	6.7e-58	92.86	protein RALF-like 24 [Cucurbita maxima]	[more]
KAG6597269.1	8.5e-53	83.82	Protein RALF-like 31, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg06309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LK37	1.1e-23	53.45	Protein RALF-like 24 OS=Arabidopsis thaliana OX=3702 GN=RALFL24 PE=3 SV=1	[more]
Q2HIM9	2.8e-22	50.82	Protein RALF-like 31 OS=Arabidopsis thaliana OX=3702 GN=RALFL31 PE=3 SV=1	[more]
Q9MA62	2.5e-15	48.39	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
Q945T0	9.6e-15	53.95	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q8L9P8	1.6e-14	53.49	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of Carg06309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G2H2	5.2e-64	97.62	protein RALF-like 24 OS=Cucurbita moschata OX=3662 GN=LOC111450186 PE=3 SV=1	[more]
A0A6J1IH73	3.3e-58	92.86	protein RALF-like 24 OS=Cucurbita maxima OX=3661 GN=LOC111473473 PE=3 SV=1	[more]
A0A0A0L7V8	1.2e-52	81.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G129640 PE=3 SV=1	[more]
A0A1S3AWQ1	2.7e-52	80.16	protein RALF-like 24 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=3 SV=...	[more]
A0A5D3CZU4	2.7e-52	80.16	Protein RALF-like 24 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of Carg06309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23805.1	8.0e-25	53.45	ralf-like 24	[more]
AT4G13950.1	2.0e-23	50.82	ralf-like 31	[more]
AT3G05490.1	1.8e-16	48.39	ralf-like 22	[more]
AT4G15800.1	1.2e-15	53.49	ralf-like 33	[more]
AT1G02900.1	5.7e-15	54.79	rapid alkalinization factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 60..121 e-value: 5.7E-28 score: 97.7
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 9..125
None	No IPR available	PANTHER	PTHR33136:SF36	PROTEIN RALF-LIKE 31	coord: 9..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06309-RA	Carg06309-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region