Carg06272 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg06272
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectate lyase
LocationCarg_Chr06: 8171712 .. 8175910 (+)
RNA-Seq ExpressionCarg06272
SyntenyCarg06272
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGCCATTTCGTTGGGAGCTTACTCTGACTCCATTACTGCCTTCTACATCTTCTTCTTCTTCTTCGTCATTTTCGTTCTCATTTCTGAACATTTGTGTCCACATTGAATTGAAGTTGAAGGAATGGAAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCGTCGAAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGTATGAATCTCTCATTCTCGCTCTAAAATTTTTGGAGTTGATGTTTTGTTCATCATAATTCTTATCTTTGTTCTCTGTCGGCGATTTCTTCTCCTTTTCTGTTATTTGCTCTGTATTTAGGGGATCCGAATTCTCTCTGTTCTTCTTCCTGGTTCGGTATTGATTTTAGCCACATTATGTTACTAGACGATCGATTTTGTTGAATTTTTAGAGTGTTGTTGTTTTCTGAATTCGAAGATTCTTCAATTTTTCGTGTGTGGTTATGTTTACTCAATCCCATTGCATTCACGCTGCTTCACTCGGAGATTTTCTTCTTTGAGCCGCTGAATTTATGTTAGGGCTCGTCCTCGGAATCATTTCAGAAGTGTGTTGTTGTCTTGATTTGTATATTTCGACCTAACGAATATTTCAGTTCGGTGATAGAGAAAATGCGTGGGAAAATGGAGATTGAATACTCCTTCTTCAGACTTTAATCTCTGTTTTTAACTTGTTATTTCAATTAATTTTGCAGGAATCGAGCAACCGAGATATTGCGGAGCTCAAGCAATGCGTCAATGGCAGAGAGGTGAATTATTTTTTTTCCTTGTTCGTCGTTTCTGTAAAAACTTGATAAATGGAGAAGATTCAGTACCTAACCAAACCCCTTCCGTCGTTAATACTTAATATCCTCCCACCTTCAATTTTCTCAACTCTATTGCCAATCTCCATCGTTTAGATCTAGATCCATGGATCTCAATTGTTTATTTCGTTGCCAGTTACTTACTCGGATTCCTCTACTCCATTAACAAATTTAAAACTCCACCTAGATTTGCAACTTTTTCCCCTTTTATGTTATTTGTAATGACAAAAAGATTTTGACATTTTCACTTTTGAAAGTGTGTGTGAAAACGGACTTCTTCCGCTCGCACTATATTCAATATTCGCTCTGAGAAAAGGCCCCTTGTAACGGTTTTTTTCGCAGCGAGCGAGCTGTTCGAGTAATCTTTTTACACTTTGAGCGACGCACAACTTTCAATAAATTGGACACTGTCGTACGTGATGTGAAAAGAAAAAACGCGTTGTATTATACATCATTCTTTCTTCTAGCACTCGCTTTGTGTGTTTGCCACGTTTTGCTTTATGATTTGCTGCCCACTGCTGTTTCCCGAAGTTGCATGTGCCCCTGTCCTGGTCACAATGCATTATTGTCCCTGCACAAAATCGATATCGTGCCTGTTAACTGTCGGTTCTAAATTATTATTCGTTTCTTCTTTTCTTTTGGGTCATTTTTTTTCTCCATTGGATCTGATACATGGGGATGGTAATTTCAGGTTGGAGGACGTCGAGGCTCTAGGTAATAAGCATGCCGTGGACAACCCTGAAGAGGTCGCTGCTGCGGTTGACATGTGGGTCCTCTTTTCCTCTTCATATAGATTATAAAATGTCAATTTGCACTATCTATGGTCTAGAATTTTGAGTTATCTATTGGGAAACTATCTGGGTGCGAATTAGGCTCAACTGCCCCGAAGCTGGTCTTCTGGGATCAAATTTTCAAGGGAAATTAAGTAAACCTATCCATTTGACCAAATTTTGCAAACCCACCTTAAAAAGATCACTAATAATTTGCCCATAACCACTTTTCTGTTCATCATCGTTAGGTTTTCGGCTTGATTCCTGAGTTGGAATCCCTAATTTCATCTTTTGTCTCTCCTTAGTCCTTAACGACGCCTTAGATTCCCAACTAGGGGTCCGTTCATTACTCTTATCTATTTCATTCTGCTTTTGCCTTTTGGTCTTATCTCGGAAAAGAACAGAAAACATCTAATGTGGATATATTTATTTCTAGGATCTTGTTATCCAGTCCATGTGGACTTGTAAAATGAGGCAAATGATCTTTTAAGTCGATTTGGTTGAGTTTATTGCAAGTGGCAACTGCCCTTGAGTGGCCCAATAGGCGACAACGACCCTAAGAAAATGGCCACCAAACTTTCATCCATCAAACTGCAGAGTCCTTCACAAATTGACAAATGGGATTACTTTATTCTATGGTTCCCATCAACCCGTTCTTGTTTTGGTCTAAGTTTAGAGATATAAGAAATTTACCCCACCGACGTTGCAAGCAAGAGTTTTGGTCTTGTAGTTAATTCTGTAAAACTTTTATAACATCGAAGGTTAACAGTTAAAAACAATACACTTGATCAGGAGCATACGCAATGCCACTGCAAGGAGGGAGCTTGGATTCTTCTCGTGTGGAACCGGCAATCCCATTGATGATTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTTGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGTGGTCGCGACGGTCGATACTATGTCGTCACTGATTCCGGCAACGACAACCCTGTCAGTCCAAGGCCTGGCACTTTACGACATGCCGTCATCCAAGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTGAAACAGGAACTGATCATGAACAGTTTCAAGACAATTGACGCTCGTGGAACCAATGTTCACATTGCTAATGGTGCTTGCATCACCATCCAGTTTGTGACTAATGTTATTGTCCATGGTCTAAATATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCACCCAATCACGTTGGGTGGAGAACAATGGCTGATGGAGATGCCATTTCCATTTTTGGGTCAAGTCATATTTGGATCGATCATAATTCACTCTCTAACTGTGCTGATGGCCTTGTTGATGCTGTCATGGGATCCACTGCAATTACAATCACCAACAATTACTTCACCCACCACAATGAGGTATCTTCACTTCATCCATCTACTCAGTTCATATTTTTGCCATTTTTCATAGACATTGCTTCACGTTGTGTGTTTAATGAACAGGTAATGTTATTGGGACATAGTGATTCATACGTTAGGGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTACAACATCTTATAATTGATCATGTTTAGCTATTTATGAGCAAGAAAACAATCCGTTTTCATCTTACAAAATAGGATCAATTTATGGATTATTTAGGTGTAGACATGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGGGGTAGTGCAAATCCTACTATCAATAGCCAGGGTAACAGATATGCTGCACCAACCAACCCTTTTGCAAAAGAGGTACTAGATACTAATAGTTTAACCTTTTCAAAATCGCTCGAGTTTAGTAGTAATTTAACCTGATATCAGAGCAAGCGGTTTAGAACTAACCTTCCTCGACATTCTTTAGGTTACCAAGAGGGTTGAGACAGCTGAATCTGAGTGGAAAAACTGGAATTGGAGATCAGAGGGAGACCTATTGATGAATGGTGCCTTTTTCACTCCGTCTGGTGCAGGGGCCTCAGCAAGCTATGCTAGAGCCTCTAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACAGCCAACGCCGGTGCACTTAACTGCCGACGAGGTCACGCCTGCTAGCATTCCGATCTCGTCGTCTATCACTTATATGTGAGTTTTTCAAGTATTTACTTACAAACAGCCTTACCCAATATCCATATTCTATCATTTCATATCTTCTTCCAACTCTCTCATATCTAAAAAACAAGTGTACATTTTCTCGTCTCGTTGCCGAGACGATTCCCTATTCGATGTGTCCCGGTTTGCTGTTTGTTGAGACACTCTGAAATCGCTCAACCTCGGCCTGCTTTAACTTGAAAAAGACATCATTTGGCATTCAGCAAGCAGGTGCTGAAGCGTTGTTCTTGTGTTCATTTATTCCCTTCTTTGGTTCCCCACTTTTGTTCTTCCCAACCCATTCCTCAAACTCTCATTGTTGCTGTAATGTTCTGTCTTCTTGTTCCTATAG

mRNA sequence

AAAAAGCCATTTCGTTGGGAGCTTACTCTGACTCCATTACTGCCTTCTACATCTTCTTCTTCTTCTTCGTCATTTTCGTTCTCATTTCTGAACATTTGTGTCCACATTGAATTGAAGTTGAAGGAATGGAAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCGTCGAAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGAATCGAGCAACCGAGATATTGCGGAGCTCAAGCAATGCGTCAATGGCAGAGAGGTTGGAGGACGTCGAGGCTCTAGGTAATAAGCATGCCGTGGACAACCCTGAAGAGGTCGCTGCTGCGGTTGACATGAGCATACGCAATGCCACTGCAAGGAGGGAGCTTGGATTCTTCTCGTGTGGAACCGGCAATCCCATTGATGATTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTTGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGTGGTCGCGACGGTCGATACTATGTCGTCACTGATTCCGGCAACGACAACCCTGTCAGTCCAAGGCCTGGCACTTTACGACATGCCGTCATCCAAGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTGAAACAGGAACTGATCATGAACAGTTTCAAGACAATTGACGCTCGTGGAACCAATGTTCACATTGCTAATGGTGCTTGCATCACCATCCAGTTTGTGACTAATGTTATTGTCCATGGTCTAAATATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCACCCAATCACGTTGGGTGGAGAACAATGGCTGATGGAGATGCCATTTCCATTTTTGGGTCAAGTCATATTTGGATCGATCATAATTCACTCTCTAACTGTGCTGATGGCCTTGTTGATGCTGTCATGGGATCCACTGCAATTACAATCACCAACAATTACTTCACCCACCACAATGAGGTAATGTTATTGGGACATAGTGATTCATACGTTAGGGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTGTAGACATGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGGGGTAGTGCAAATCCTACTATCAATAGCCAGGGTAACAGATATGCTGCACCAACCAACCCTTTTGCAAAAGAGGTTACCAAGAGGGTTGAGACAGCTGAATCTGAGTGGAAAAACTGGAATTGGAGATCAGAGGGAGACCTATTGATGAATGGTGCCTTTTTCACTCCGTCTGGTGCAGGGGCCTCAGCAAGCTATGCTAGAGCCTCTAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACAGCCAACGCCGGTGCACTTAACTGCCGACGAGGTCACGCCTGCTAGCATTCCGATCTCGTCGTCTATCACTTATATGTGAGTTTTTCAAGTATTTACTTACAAACAGCCTTACCCAATATCCATATTCTATCATTTCATATCTTCTTCCAACTCTCTCATATCTAAAAAACAAGTGTACATTTTCTCGTCTCGTTGCCGAGACGATTCCCTATTCGATGTGTCCCGGTTTGCTGTTTGTTGAGACACTCTGAAATCGCTCAACCTCGGCCTGCTTTAACTTGAAAAAGACATCATTTGGCATTCAGCAAGCAGGTGCTGAAGCGTTGTTCTTGTGTTCATTTATTCCCTTCTTTGGTTCCCCACTTTTGTTCTTCCCAACCCATTCCTCAAACTCTCATTGTTGCTGTAATGTTCTGTCTTCTTGTTCCTATAG

Coding sequence (CDS)

ATGGAAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCGTCGAAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGAATCGAGCAACCGAGATATTGCGGAGCTCAAGCAATGCGTCAATGGCAGAGAGGTTGGAGGACGTCGAGGCTCTAGGTAATAAGCATGCCGTGGACAACCCTGAAGAGGTCGCTGCTGCGGTTGACATGAGCATACGCAATGCCACTGCAAGGAGGGAGCTTGGATTCTTCTCGTGTGGAACCGGCAATCCCATTGATGATTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTTGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGTGGTCGCGACGGTCGATACTATGTCGTCACTGATTCCGGCAACGACAACCCTGTCAGTCCAAGGCCTGGCACTTTACGACATGCCGTCATCCAAGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTGAAACAGGAACTGATCATGAACAGTTTCAAGACAATTGACGCTCGTGGAACCAATGTTCACATTGCTAATGGTGCTTGCATCACCATCCAGTTTGTGACTAATGTTATTGTCCATGGTCTAAATATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCACCCAATCACGTTGGGTGGAGAACAATGGCTGATGGAGATGCCATTTCCATTTTTGGGTCAAGTCATATTTGGATCGATCATAATTCACTCTCTAACTGTGCTGATGGCCTTGTTGATGCTGTCATGGGATCCACTGCAATTACAATCACCAACAATTACTTCACCCACCACAATGAGGTAATGTTATTGGGACATAGTGATTCATACGTTAGGGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTGTAGACATGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGGGGTAGTGCAAATCCTACTATCAATAGCCAGGGTAACAGATATGCTGCACCAACCAACCCTTTTGCAAAAGAGGTTACCAAGAGGGTTGAGACAGCTGAATCTGAGTGGAAAAACTGGAATTGGAGATCAGAGGGAGACCTATTGATGAATGGTGCCTTTTTCACTCCGTCTGGTGCAGGGGCCTCAGCAAGCTATGCTAGAGCCTCTAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACAGCCAACGCCGGTGCACTTAACTGCCGACGAGGTCACGCCTGCTAG

Protein sequence

MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEALGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC
Homology
BLAST of Carg06272 vs. NCBI nr
Match: KAG7028778.1 (putative pectate lyase 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 912.5 bits (2357), Expect = 1.4e-261
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. NCBI nr
Match: KAG6597311.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.2 bits (2351), Expect = 6.9e-261
Identity = 446/447 (99.78%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           M MAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MAMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. NCBI nr
Match: XP_022974867.1 (probable pectate lyase 8 [Cucurbita maxima])

HSP 1 Score: 907.9 bits (2345), Expect = 3.4e-260
Identity = 444/447 (99.33%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           M MAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MAMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGND+P+SPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDDPISPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. NCBI nr
Match: XP_022945913.1 (probable pectate lyase 8 [Cucurbita moschata])

HSP 1 Score: 907.5 bits (2344), Expect = 4.5e-260
Identity = 445/447 (99.55%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           MEMAESGGWIRLFLLVVFMLFVETIAR PKEI EIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MEMAESGGWIRLFLLVVFMLFVETIARAPKEIHEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. NCBI nr
Match: XP_038895013.1 (probable pectate lyase 8 [Benincasa hispida])

HSP 1 Score: 861.3 bits (2224), Expect = 3.7e-246
Identity = 416/447 (93.06%), Postives = 435/447 (97.32%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           M MAESGGW RLFLLVVFMLFV TIARVPKEIPEI+N ATE +RSSSN+SMA RL++ EA
Sbjct: 1   MTMAESGGWTRLFLLVVFMLFVGTIARVPKEIPEIQNGATEKMRSSSNSSMAVRLDEAEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEE+AA VDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGR+Y+VTDSG+DNPV+P+PGTLRHAVIQDEPLWIVFKRDMVI LKQ
Sbjct: 121 CGIGFGRNAIGGRDGRFYMVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIHLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI+NNYFTHHNEVMLLGHS
Sbjct: 241 RTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAE+EWKNWNWRSEGDLL+NGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAETEWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMV +IT++AGAL+CRRGH C
Sbjct: 421 LGAKSSSMVGTITSDAGALSCRRGHRC 447

BLAST of Carg06272 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 689.9 bits (1779), Expect = 1.9e-197
Identity = 333/404 (82.43%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 48  NASMAERLEDVEALGNKHAVDNPEEVA----AAVDMSIRNATARRELGFFSCGTGNPIDD 107
           N S   R ++ E   N+HAV NP+EVA    A  +MS+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETE--WNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 108 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQD 167
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGR+YVVTD  +DNPV+PRPGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 168 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDC 227
            PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG CITIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 228 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 287
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 288 TNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTH 347
           +NN+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTH
Sbjct: 268 SNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTH 327

Query: 348 WEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAF 407
           WEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  S WK WNWRSEGDLL NGA+
Sbjct: 328 WEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAY 387

Query: 408 FTPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC 448
           FT SGA AS SYARASSL AKSSS+V  IT++AGAL CRRG  C
Sbjct: 388 FTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Carg06272 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 6.2e-196
Identity = 332/418 (79.43%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 35  IENRATEILRSSSNASMAERLEDVEALGNKHAV-DNP----EEVAAAVDMSIRNATARRE 94
           I    TE  +S ++++MA    D E    +HAV D+P    +EVA  V MS +N TARR+
Sbjct: 53  IRKLKTEEFQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRK 112

Query: 95  LGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPV 154
           LGFFSCGTGNPIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGR+Y+VTD  +++ V
Sbjct: 113 LGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVV 172

Query: 155 SPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQF 214
           +P+PGTLRHAVIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGACITIQF
Sbjct: 173 NPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQF 232

Query: 215 VTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCAD 274
           +TNVI+HGL+IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CAD
Sbjct: 233 ITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCAD 292

Query: 275 GLVDAVMGSTAITITNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRC 334
           GLVDAVMGSTAIT++NN+FTHHNEVMLLGHSDSY +DK MQVTIAYNHFGEGL+QRMPRC
Sbjct: 293 GLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRC 352

Query: 335 RHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNW 394
           RHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  SEWK W
Sbjct: 353 RHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKW 412

Query: 395 NWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC 448
           NWRSEGDLL+NGAFF PSGAGASASY RASSL AK SSMV +IT+ AGAL CR+G  C
Sbjct: 413 NWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of Carg06272 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 9.0e-195
Identity = 319/385 (82.86%), Postives = 352/385 (91.43%), Query Frame = 0

Query: 63  NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NP+EVAA VDMSIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 123 IGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGR+YVVTD G+D+PV+P PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGAC+TIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDS 302
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT++NN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 363 RYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLG 422
           R+ AP NPFAKEVTKR  T ES+WK+WNWRSEGDL +NGAFFT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 423 AKSSSMVASITANAGALNCRRGHAC 448
           AKSSS+V ++T+ +GALNCR G  C
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Carg06272 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.3e-193
Identity = 318/385 (82.60%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 63  NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NPEEVAA VDMSIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 123 IGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGRYYVV+D  +DNPV+P+PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGACITIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDS 302
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI+NNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 363 RYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLG 422
           RY AP N FAKEVTKR    + +W++WNWRSEGDL +NGAFFT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 423 AKSSSMVASITANAGALNCRRGHAC 448
           AKSSS+V  IT NAGALNCR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of Carg06272 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 668.7 bits (1724), Expect = 4.6e-191
Identity = 317/403 (78.66%), Postives = 352/403 (87.34%), Query Frame = 0

Query: 45  SSSNASMAERLEDVEALGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDC 104
           SSSN+S A +L + +   N+HAV NPEEVAA VDM I+N+T RR LGFFSC TGNPIDDC
Sbjct: 38  SSSNSSTANKLPNDDGAWNEHAVKNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDC 97

Query: 105 WRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDE 164
           WRCD NW   RKRLA+C IGFGRNAIGGRDGRYYVVTD  + + V+PRPGTLRHAVIQD 
Sbjct: 98  WRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDR 157

Query: 165 PLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCK 224
           PLWIVFKRDMVI L QELIMNSFKTID RG NV IA GACITIQ+VTN+I+HG+N+HDC+
Sbjct: 158 PLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCR 217

Query: 225 PTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITIT 284
            TGNAMVRSSP+H GWRTMADGDAISIFGSSHIWIDHNSLSNCADGL+DA+MGSTAITI+
Sbjct: 218 RTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITIS 277

Query: 285 NNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW 344
           NNY THHNEVML+GHSDSY RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW
Sbjct: 278 NNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW 337

Query: 345 EMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFF 404
            MYAIGGSANPTINSQGNR+ AP NPFAKEVTKRV + + EWK WNWRS+GDL++NGA+F
Sbjct: 338 VMYAIGGSANPTINSQGNRFLAPGNPFAKEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYF 397

Query: 405 TPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC 448
           T SGA A ASYARASSLGAK +S+V+ +T ++GAL CR G  C
Sbjct: 398 TKSGAAAPASYARASSLGAKPASVVSMLTYSSGALKCRIGMRC 440

BLAST of Carg06272 vs. ExPASy TrEMBL
Match: A0A6J1IBF4 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111473637 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.7e-260
Identity = 444/447 (99.33%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           M MAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MAMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGND+P+SPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDDPISPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. ExPASy TrEMBL
Match: A0A6J1G2B9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450013 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.2e-260
Identity = 445/447 (99.55%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           MEMAESGGWIRLFLLVVFMLFVETIAR PKEI EIENRATEILRSSSNASMAERLEDVEA
Sbjct: 1   MEMAESGGWIRLFLLVVFMLFVETIARAPKEIHEIENRATEILRSSSNASMAERLEDVEA 60

Query: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 421 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVASITANAGALNCRRGHAC
Sbjct: 421 LGAKSSSMVASITANAGALNCRRGHAC 447

BLAST of Carg06272 vs. ExPASy TrEMBL
Match: A0A6J1EAL4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111432214 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 420/449 (93.54%), Postives = 434/449 (96.66%), Query Frame = 0

Query: 1   MEMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEA 60
           M MAESGGWIRLFLLVVFMLFV  IARVPKEIPEI N AT  L+SSSN+SMA RLE+ EA
Sbjct: 1   MAMAESGGWIRLFLLVVFMLFVGAIARVPKEIPEIHNEATGKLQSSSNSSMAVRLEEPEA 60

Query: 61  --LGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL 120
              GNKHAVDNP+E+AA VDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL
Sbjct: 61  ETPGNKHAVDNPDEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL 120

Query: 121 ADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQL 180
           ADCGIGFGRNAIGGRDGR+YV+TDSG+D+PV+PRPGTLRHAVIQDEPLWIVFKRDMVIQL
Sbjct: 121 ADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQL 180

Query: 181 KQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV 240
           KQELIMNSFKTIDARG+NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV
Sbjct: 181 KQELIMNSFKTIDARGSNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLG 300
           GWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLG
Sbjct: 241 GWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLG 300

Query: 301 HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARA 420
           SQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARA 420

Query: 421 SSLGAKSSSMVASITANAGALNCRRGHAC 448
           SSLGAKSSSMVA+ITA AGAL+CRRGH C
Sbjct: 421 SSLGAKSSSMVATITATAGALSCRRGHPC 449

BLAST of Carg06272 vs. ExPASy TrEMBL
Match: A0A6J1IVV8 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479711 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.3e-244
Identity = 416/447 (93.06%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 3   MAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEALG 62
           MAESGGWIRLFLLVVF+LFV  IARVPK+IPEI+N AT  L+SSSN+SMA RLE+ EA  
Sbjct: 1   MAESGGWIRLFLLVVFLLFVGAIARVPKDIPEIDNEATGKLQSSSNSSMAVRLEEPEAET 60

Query: 63  --NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 122
             NKHAVDNP+E+AA VDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 61  PVNKHAVDNPDEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120

Query: 123 CGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 182
           CGIGFGRNAIGGRDGR+YV+TDSG+D+PV+PRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 121 CGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180

Query: 183 ELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 242
           ELIMNSFKTIDARG+NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 181 ELIMNSFKTIDARGSNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240

Query: 243 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 302
           RT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS
Sbjct: 241 RTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHS 300

Query: 303 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 362
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360

Query: 363 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 422
           GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS
Sbjct: 361 GNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASS 420

Query: 423 LGAKSSSMVASITANAGALNCRRGHAC 448
           LGAKSSSMVA+ITA AGAL+CRRGH C
Sbjct: 421 LGAKSSSMVATITATAGALSCRRGHPC 447

BLAST of Carg06272 vs. ExPASy TrEMBL
Match: A0A6J1CZC3 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111016150 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.4e-243
Identity = 414/445 (93.03%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 3   MAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEALG 62
           MA SG WIRL LLVVFMLFV TIARVPKEI + +N ATE+L+SSSN+SMA RL++VEAL 
Sbjct: 68  MAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEALD 127

Query: 63  NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           NKHAVDNPEE+AA VDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG
Sbjct: 128 NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 187

Query: 123 IGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGR+YVVTDSG+DNPV+P PGTLRHAVIQDEPLWIVFKRDMVIQLKQEL
Sbjct: 188 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 247

Query: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTIDARG NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT
Sbjct: 248 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 307

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDS 302
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITIT+NYFTHHNEVMLLGHSDS
Sbjct: 308 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 367

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN
Sbjct: 368 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 427

Query: 363 RYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLG 422
           RYAAPTNPFAKEVTKRVETAE+EWK+WNWRSEGDLL+NGAFFTPSGAGASASYARASSLG
Sbjct: 428 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 487

Query: 423 AKSSSMVASITANAGALNCRRGHAC 448
           AKSSSMVASIT++AGAL+CRRGH C
Sbjct: 488 AKSSSMVASITSSAGALSCRRGHPC 512

BLAST of Carg06272 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 689.9 bits (1779), Expect = 1.4e-198
Identity = 333/404 (82.43%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 48  NASMAERLEDVEALGNKHAVDNPEEVA----AAVDMSIRNATARRELGFFSCGTGNPIDD 107
           N S   R ++ E   N+HAV NP+EVA    A  +MS+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETE--WNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 108 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQD 167
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGR+YVVTD  +DNPV+PRPGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 168 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDC 227
            PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG CITIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 228 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 287
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 288 TNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTH 347
           +NN+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTH
Sbjct: 268 SNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTH 327

Query: 348 WEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAF 407
           WEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  S WK WNWRSEGDLL NGA+
Sbjct: 328 WEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAY 387

Query: 408 FTPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC 448
           FT SGA AS SYARASSL AKSSS+V  IT++AGAL CRRG  C
Sbjct: 388 FTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Carg06272 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 684.9 bits (1766), Expect = 4.4e-197
Identity = 332/418 (79.43%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 35  IENRATEILRSSSNASMAERLEDVEALGNKHAV-DNP----EEVAAAVDMSIRNATARRE 94
           I    TE  +S ++++MA    D E    +HAV D+P    +EVA  V MS +N TARR+
Sbjct: 53  IRKLKTEEFQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRK 112

Query: 95  LGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPV 154
           LGFFSCGTGNPIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGR+Y+VTD  +++ V
Sbjct: 113 LGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVV 172

Query: 155 SPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQF 214
           +P+PGTLRHAVIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGACITIQF
Sbjct: 173 NPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQF 232

Query: 215 VTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCAD 274
           +TNVI+HGL+IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CAD
Sbjct: 233 ITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCAD 292

Query: 275 GLVDAVMGSTAITITNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRC 334
           GLVDAVMGSTAIT++NN+FTHHNEVMLLGHSDSY +DK MQVTIAYNHFGEGL+QRMPRC
Sbjct: 293 GLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRC 352

Query: 335 RHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESEWKNW 394
           RHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  SEWK W
Sbjct: 353 RHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKW 412

Query: 395 NWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMVASITANAGALNCRRGHAC 448
           NWRSEGDLL+NGAFF PSGAGASASY RASSL AK SSMV +IT+ AGAL CR+G  C
Sbjct: 413 NWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of Carg06272 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.2e-196
Identity = 336/443 (75.85%), Postives = 379/443 (85.55%), Query Frame = 0

Query: 10  IRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEALGNKHAV-D 69
           I + + V+ +L +  +     EI       TE  +S ++++MA    D E    +HAV D
Sbjct: 9   ISVCVAVLVVLALTAMIFRNSEISLSRKLKTEEFQSLNSSTMAATRLDGEPQQQQHAVAD 68

Query: 70  NP----EEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIG 129
           +P    +EVA  V MS +N TARR+LGFFSCGTGNPIDDCWRCD NW +NRKRLADCGIG
Sbjct: 69  DPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADCGIG 128

Query: 130 FGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIM 189
           FGRNAIGGRDGR+Y+VTD  +++ V+P+PGTLRHAVIQ+EPLWIVFKRDMVI+LKQELIM
Sbjct: 129 FGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQELIM 188

Query: 190 NSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMA 249
           NSFKTIDARG+NVHIANGACITIQF+TNVI+HGL+IHDCKPTGNAMVRSSP+H GWRTMA
Sbjct: 189 NSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMA 248

Query: 250 DGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDSYV 309
           DGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT++NN+FTHHNEVMLLGHSDSY 
Sbjct: 249 DGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYT 308

Query: 310 RDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRY 369
           +DK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRY
Sbjct: 309 KDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRY 368

Query: 370 AAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAK 429
           AAP + FAKEVTKRVET  SEWK WNWRSEGDLL+NGAFF PSGAGASASY RASSL AK
Sbjct: 369 AAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAK 428

Query: 430 SSSMVASITANAGALNCRRGHAC 448
            SSMV +IT+ AGAL CR+G  C
Sbjct: 429 PSSMVDTITSTAGALGCRKGRPC 451

BLAST of Carg06272 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 681.0 bits (1756), Expect = 6.4e-196
Identity = 319/385 (82.86%), Postives = 352/385 (91.43%), Query Frame = 0

Query: 63  NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NP+EVAA VDMSIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 123 IGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGR+YVVTD G+D+PV+P PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGAC+TIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDS 302
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT++NN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 363 RYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLG 422
           R+ AP NPFAKEVTKR  T ES+WK+WNWRSEGDL +NGAFFT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 423 AKSSSMVASITANAGALNCRRGHAC 448
           AKSSS+V ++T+ +GALNCR G  C
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Carg06272 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 677.2 bits (1746), Expect = 9.2e-195
Identity = 318/385 (82.60%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 63  NKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NPEEVAA VDMSIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 123 IGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGRYYVV+D  +DNPV+P+PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGACITIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSDS 302
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI+NNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 363 RYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLG 422
           RY AP N FAKEVTKR    + +W++WNWRSEGDL +NGAFFT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 423 AKSSSMVASITANAGALNCRRGHAC 448
           AKSSS+V  IT NAGALNCR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028778.11.4e-261100.00putative pectate lyase 15 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6597311.16.9e-26199.78putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022974867.13.4e-26099.33probable pectate lyase 8 [Cucurbita maxima][more]
XP_022945913.14.5e-26099.55probable pectate lyase 8 [Cucurbita moschata][more]
XP_038895013.13.7e-24693.06probable pectate lyase 8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q940Q11.9e-19782.43Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q944R16.2e-19679.43Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1[more]
Q9M8Z89.0e-19582.86Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1[more]
Q93WF11.3e-19382.60Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Q9LJ424.6e-19178.66Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1IBF41.7e-26099.33Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111473637 PE=3 SV=1[more]
A0A6J1G2B92.2e-26099.55Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450013 PE=3 SV=1[more]
A0A6J1EAL41.2e-24593.54Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111432214 PE=3 SV=1[more]
A0A6J1IVV88.3e-24493.06Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479711 PE=3 SV=1[more]
A0A6J1CZC31.4e-24393.03Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111016150 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G04680.11.4e-19882.43Pectin lyase-like superfamily protein [more]
AT4G13710.14.4e-19779.43Pectin lyase-like superfamily protein [more]
AT4G13710.22.2e-19675.85Pectin lyase-like superfamily protein [more]
AT3G07010.16.4e-19682.86Pectin lyase-like superfamily protein [more]
AT5G48900.19.2e-19582.60Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 325..344
score: 78.1
coord: 389..413
score: 53.52
coord: 347..366
score: 75.0
coord: 145..170
score: 61.17
coord: 121..138
score: 54.23
coord: 418..441
score: 37.5
coord: 181..197
score: 63.87
coord: 245..266
score: 69.48
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 173..370
e-value: 3.4E-102
score: 355.5
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 184..362
e-value: 2.7E-19
score: 69.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 98..440
e-value: 5.7E-117
score: 392.5
NoneNo IPR availablePANTHERPTHR31683:SF81PECTATE LYASEcoord: 13..444
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 13..444
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 73..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06272-RACarg06272-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity